Synergy of surface adsorption and intracellular accumulation for removal of uranium with Stenotrophomonas sp: Performance and mechanisms.

Uranium is well-known to have serious adverse effects on the ecological environment and human health. Bioremediation stands out among many remediation methods owing to its being economically feasible and environmentally friendly. This study reported a great promising strategy for eliminating uranium by Stenotrophomonas sp. CICC 23833 in the aquatic environment. The bacterium demonstrated excellent uranium adsorption capacity (qmax = 392.9 mg/g) because of the synergistic effect of surface adsorption and intracellular accumulation. Further analysis revealed that hydroxyl, carboxyl, phosphate groups and proteins of microorganisms were essential in uranium adsorption. Intracellular accumulation was closely related to cellular activity, and the efficiency of uranium processing by the permeabilized bacterial cells was significantly improved. In response to uranium stress, the bacterium was found to release multiple ions in conjunction with uranium adsorption, which facilitates the maintenance of bacterial life activities and the conversion of uranyl to precipitates. These above results indicated that Stenotrophomonas sp. Had great potential application value for the remediation of uranium.

Potato peel waste for fermentative biohydrogen production using different pretreated culture.

How to manage potato peel waste sustainably has been an issue faced by the potato industry. This work explored the feasibility of potato peel waste for biohydrogen production via dark fermentation, and investigated the effects of various inoculum enrichment methods (acid, aeration, heat-shock and base) on the process efficiency. It was observed that the hydrogen production showed a great variation when using various inoculum enrichment methods, and the aeration enriched inoculum obtained the maximum hydrogen yield of 71.0 mL/g-VSadded and VS removal of 28.9 %. Different enriched cultures also exhibited huge variations in the bacterial community structure and metabolic pathway. The highest abundance of Clostridium sensu stricto fundamentally contributed to the highest process efficiency for the fermenter inoculated with aeration treated culture. This work puts forward a promising strategy for recycling potato peel waste, and fills a gap in the optimal inoculum preparation method for biohydrogen fermentation of potato peel waste.

Milk vetch returning reduces rice grain Cd concentration in paddy fields: Roles of iron plaque and soil reducing-bacteria.

Milk vetch (MV, Astragalus sinicus L.) is used in agricultural production as a green manure; however, its impact on accumulation levels of heavy metals (e.g., Cd) in rice remains poorly understood. This study investigated the effects of MV on Cd accumulation in rice, iron plaque formation, soil properties, and the soil microbial community structure through field experiments. The results showed that MV reduced Cd concentration in the roots, stem, leaves, and grains by 33%, 60%, 71%, and 49%, respectively. Chemical fertilizer and MV treatment promoted iron plaque formation, and MV considerably increased the Fe/Mn ratio in the iron plaque. More importantly, MV inhibited Cd transportation from the root iron plaque to the root by 74%. The concentrations of CaCl2-extractable Cd, available phosphorus, and available potassium, as well as the cation exchange capacity and urease activity, were significantly reduced in the MV treatment. Furthermore, 16 S rDNA high-throughput sequencing results of the soil microbial community structure showed that compared with the control, MV increased the soil microbial richness, increased the relative abundance of anaerobic microorganisms, and significantly increased the relative abundance of Thermodesulfovibrio and Geobacter at the genus level. The increase in anaerobic microbial abundance was closely related to the decrease in CaCl2-extractable Cd concentration. The application of MV promoted the formation of iron plaque, inhibited the transport of Cd, increased the abundance of anaerobic microorganisms, decreased the CaCl2-extractable Cd concentration, and reduced the Cd concentration in rice grain.

Metagenomic insights into improving mechanisms of Fe0 nanoparticles on volatile fatty acids production from potato peel waste anaerobic fermentation.

The management of potato peel waste (PPW) has been a challenge faced by the potato industry. This investigation assessed the feasibility of PPW for volatile fatty acids (VFAs) production via anaerobic fermentation, and investigated the impact of Fe0 nanoparticles (Fe0 NPs) supplementation on the VFAs production. It is found that PPW is a potential feedstock for producing VFAs, achieving a yield of 480.4 mg COD/g-vS Meanwhile, the supplementation of Fe0 NPs significantly promoted the VFAs productivity and quality. The higher enrichment of VFAs-producing bacteria, including Clostridium, Proteiniphilum, Fonticella and Pygmaiobacter, contributed to the promotion of the VFAs yield. Furthermore, metagenomic analysis revealed that the encoding genes responsible for carbohydrate metabolism (especially starch), membrane transport, glycolysis and the formation of acetic and butyric acids were remarkably up-regulated,which could be the essential reason for the enhanced metabolic activity and VFAs productivity. This work provides a promising strategy for recycling PPW.

Silicon fertilizers, humic acid and their impact on physicochemical properties, availability and distribution of heavy metals in soil and soil aggregates.

It has been confirmed that silicon (Si) fertilizer and humic acid (HA) could effectively decrease the heavy metals in soil. Nonetheless, the impact of these additives on soil aggregate characteristics was ignored. Therefore, the effects of Si fertilizer, HA, and their combinations on the physicochemical characteristics, availability of heavy metals (Cu, Cd, Pb, Zn), and fraction changes in soils and soil aggregates were investigated in this research. The results showed that Si fertilizer and HA significantly modified soil properties such as soil pH, electrical conductivity total organic carbon, water-soluble organic carbon, and nitrate­nitrogen. HA and Si-HA (SHA) supplementation significantly decreased the availability of Cu, Cd, Pd, and Zn. Besides, there was no significant difference in physicochemical properties between soil and soil aggregates. The availability of Cu, Cd, Pd, and Zn in soil aggregates could be significantly inhibited by the addition of HA and SHA, and the content in microaggregates was greater than that in macroaggregates. After the addition of the three additives, the main fractions of heavy metals in different particle sizes were changed and eventually transformed to the residue state. These results indicated that Si fertilizer, HA, and SHA were influential in physicochemical properties and metal availability in soil aggregates. Therefore, it is of great scientific significance to study the impact of heavy metal pollution on the ecological environment in different aggregates, which will provide reference data for future sustainable management of heavy-metal polluted soils.

Monitoring the nitrous oxide emissions and biological nutrient removal from wastewater treatment: Impact of perfluorooctanoic acid.

The impacts of perfluorooctanoic acid (PFOA) on biological nutrient removal and nitrous oxide (N2O) emissions have been specifically studied. The experimental results show that PFOA inhibited nitrification, but promoted denitrification and reduced N2O emissions without significantly affecting phosphorus removal. The existence of 20 mg/L of PFOA increased total nitrogen removal efficiency from 78.7 ± 6.89 % to 86.8 ± 6.39 % and reduced N2O emission factor from 6.02 ± 0.24 % to 4.43 ± 0.10 %. The mechanism studies reveal that microorganisms released extracellular polymeric substances (EPS) under PFOA exposure to protect sludge cells against PFOA toxicity. The generated PFOA-EPS conjugates reduced the nitrification rate, but increased the denitrification rate by regulating the activity of oxidoreductases. In addition, PFOA reduced the activity of polyphosphate accumulating organisms and glycogen accumulating organisms to save carbon source for denitrification, which reduced the electronic competition between reductases, thereby achieving complete denitrification and N2O mitigation. The promotion of PFOA for denitrification and N2O mitigation can gain a more comprehensive cognition of the role of PFOA in wastewater treatment. The release mechanism of EPS can afford new insights for the development of effective methods to enhance nitrogen removal and reduce N2O emissions.

Self-powered photoelectrochemical aptasensor based on phosphorus doped porous ultrathin g-C3N4 nanosheets enhanced by surface plasmon resonance effect.

This work reports a facile and sensitive self-powered cathodic photoelectrochemical (PEC) aptasensor for the detection of oxytetracycline (OTC) based on Au nanoparticles-decorated phosphorus-doped porous ultrathin g-C3N4 nanosheets (Au/PCN-S). The prepared PCN-S possesses large specific surface area with abundant in-plane pores on its surface, ideal biocompatibility, and excellent visible light response. The in situ photo-reduced Au nanoparticles further enhanced the PEC performance owing to its unique surface plasmon resonance (SPR) effect. Under visible light irradiation, the photocurrent of Au/PCN-S composites was significantly enhanced, which was about 22 times higher than that of pure g-C3N4. In the self-powered PEC biosensing of OTC, the device exhibited high sensitivity toward the presence of dissolved oxygen in the electrolyte and presented a wide detection range from 0.5 to 200 nM and a detection limit of 0.34 nM, as well as certain selectivity, reproducibility and stability. The proposed Au/PCN-S nanocomposites would be considered as a promising visible light-responsive photoactive material for fabrication of PEC biosensors with high performance.

Selenium contamination, consequences and remediation techniques in water and soils: A review.

Selenium (Se) contamination in surface and ground water in numerous river basins has become a critical problem worldwide in recent years. The exposure to Se, either direct consumption of Se or indirectly may be fatal to the human health because of its toxicity. The review begins with an introduction of Se chemistry, distribution and health threats, which are essential to the remediation techniques. Then, the review provides the recent and common removal techniques for Se, including reduction techniques, phytoremediation, bioremediation, coagulation-flocculation, electrocoagulation (EC), electrochemical methods, adsorption, coprecipitation, electrokinetics, membrance technology, and chemical precipitation. Removal techniques concentrate on the advantages, drawbacks and the recent achievements of each technique. The review also takes an overall consideration of experimental conditions, comparison criteria and economic aspects.

Construction of plasmonic Ag modified phosphorous-doped ultrathin g-C3N4 nanosheets/BiVO4 photocatalyst with enhanced visible-near-infrared response ability for ciprofloxacin degradation.

To realize the full utilization of solar energy, the design of highly efficient photocatalyst with improved visible-near-infrared photocatalysis performance has attracted great attentions for environment pollutant removal. In this work, we rationally employed the surface plasmon resonance effect of metallic Ag in the phosphorus doped ultrathin g-C3N4 nanosheets (PCNS) and BiVO4 composites to construct a ternary Ag@PCNS/BiVO4 photocatalyst. It was applied for the photodegradation of ciprofloxacin (CIP), exhibiting 92.6% removal efficiency under visible light irradiation (λ>420nm) for 10mg/L CIP, and presenting enhanced photocatalytic ability than that of single component or binary nanocomposites under near-infrared light irradiation (λ>760nm). The improved photocatalytic activity of the prepared Ag@PCNS/BiVO4 nanocomposite can be attributed to the synergistic effect among the PCNS, BiVO4 and Ag, which not only improves the visible light response ability and hinders the recombination efficiency of the photogenerated electrons and holes, but also retains the strong the redox ability of the photogenerated charges. According to the trapping experiment and ESR measurements results, OH, h+ and O2- all participated in the photocatalytic degradation process. Considering the SPR effect of metallic Ag and the established local electric field around the interfaces, a dual Z-scheme electrons transfer mechanism was proposed.