RESUMO
In this study, a highly efficient phosphate-solubilizing bacteria (PSB) (Pantoea sp. grinm-12) was screened out from uranium (U) tailings, and the carbon and nitrogen sources of mixed culture with sulfate-reducing bacteria (SRB) were optimized. Results showed that the functional expression of SRB-PSB could be promoted effectively when glucoseâ¯+â¯sodium lactate was used as carbon source and ammonium nitrateâ¯+â¯ammonium sulfate as nitrogen source. The concentration of PO43- in the culture system could reach 107.27â¯mg·L-1, and the sulfate reduction rate was 81.72%. In the process of biological stabilization of U tailings by mixed SRB-PSB culture system, the chemical form of U in the remediation group was found to transfer to stable state with the extension of remediation time, which revealed the effectiveness of bioremediation on the harmless treatment of U tailings. XRD, FT-IR, SEM-EDS, high-throughput sequencing, and metagenomics were also used to assist in revealing the microstructure and composition changes during the biological stabilization process, and explore the microbial community/functional gene response. Finally, the stabilization mechanism of U was proposed. In conclusion, the stabilization of U in U tailings was realized through the synergistic effect of bio-reduction, bio-precipitation, and bio-adsorption.