RESUMO
In the face of starvation, animals will engage in high-risk behaviors that would normally be considered maladaptive. Starving rodents, for example, will forage in areas that are more susceptible to predators and will also modulate aggressive behavior within a territory of limited or depleted nutrients. The neural basis of these adaptive behaviors likely involves circuits that link innate feeding, aggression and fear. Hypothalamic agouti-related peptide (AgRP)-expressing neurons are critically important for driving feeding and project axons to brain regions implicated in aggression and fear. Using circuit-mapping techniques in mice, we define a disynaptic network originating from a subset of AgRP neurons that project to the medial nucleus of the amygdala and then to the principal bed nucleus of the stria terminalis, which suppresses territorial aggression and reduces contextual fear. We propose that AgRP neurons serve as a master switch capable of coordinating behavioral decisions relative to internal state and environmental cues.
Assuntos
Agressão/fisiologia , Proteína Relacionada com Agouti/fisiologia , Tonsila do Cerebelo/fisiologia , Medo/fisiologia , Hipotálamo/fisiologia , Fragmentos de Peptídeos/fisiologia , Núcleos Septais/fisiologia , Inanição/fisiopatologia , Proteína Relacionada com Agouti/metabolismo , Tonsila do Cerebelo/metabolismo , Animais , Técnicas de Introdução de Genes , Hipotálamo/metabolismo , Masculino , Camundongos , Vias Neurais/metabolismo , Vias Neurais/fisiologia , Neurônios/fisiologia , Fragmentos de Peptídeos/metabolismo , Núcleos Septais/metabolismoRESUMO
Generalized anxiety is thought to result, in part, from impairments in contingency awareness during conditioning to cues that predict aversive or fearful outcomes. Dopamine neurons of the ventral midbrain exhibit heterogeneous responses to aversive stimuli that are thought to provide a critical modulatory signal to facilitate orientation to environmental changes and assignment of motivational value to unexpected events. Here we describe a mouse model in which activation of dopamine neurons in response to an aversive stimulus is attenuated by conditional genetic inactivation of functional NMDA receptors on dopamine neurons. We discovered that altering the magnitude of excitatory responses by dopamine neurons in response to an aversive stimulus was associated with impaired conditioning to a cue that predicts an aversive outcome. Impaired conditioning by these mice was associated with the development of a persistent, generalized anxiety-like phenotype. These data are consistent with a role for dopamine in facilitating contingency awareness that is critical for the prevention of generalized anxiety.