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1.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2397-2401, Nov.-Dec. 2020. graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1142296

RESUMO

O presente estudo avaliou o efeito da suplementação com OmniGen-AF® na proliferação de linfócitos e títulos de anticorpos após vacinação em bovinos leiteiros. Amostras de sangue periférico foram coletadas de 32 vacas leiteiras para quantificação dos títulos de anticorpos anti-Leptospira, e amostras de sangue periférico de 16 vacas leiteiras foram também coletadas para avaliação da proliferação de linfócitos. Observou-se que a suplementação com OmniGen-AF® aumentou a proliferação basal de linfócitos (sem estímulos) 21 dias após a vacinação (P=0,03), apesar de reduzir a proliferação de linfócitos B quando estimulada com Leptospira borgpetersenii serovar Hardjo inativada pelo calor (P=0,03). Ademais, nenhum efeito da suplementação sobre a proliferação de linfócitos no momento imediatamente anterior à vacinação e nos títulos de anticorpos anti-Leptospira foi encontrado. Além disso, a proliferação de linfócitos estimulada com lipopolissacarídeos foi maior em vacas multíparas que em primíparas 21 dias após a vacinação (P=0,03). Desse modo, o presente estudo demonstra que a suplementação com OmniGen-AF® não afetou de forma robusta a proliferação de linfócitos e os títulos de anticorpos anti-Leptospira após vacinação em vacas leiteiras sadias.(AU)


Assuntos
Animais , Feminino , Bovinos , Vacinas Combinadas/análise , Suplementos Nutricionais/análise , Fatores Imunológicos/administração & dosagem , Linfocitose/veterinária , Lipopolissacarídeos , Leptospira/imunologia
2.
J Anim Sci ; 98(3)2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32144425

RESUMO

The ability to positively alter immune and stress response with nutritional compounds is of great interest and importance to the beef industry. There is a proprietary product (OmniGen-AF [OG]; Phibro Animal Health, Quincy, IL) reported to have performance-enhancing benefits by altering animal response to stress and immune challenges. The objective of this 2-yr research project was to study the effect of supplementing OG to beef cows and their calves on breeding and growth performance. One hundred and twelve multiparous beef cows and 48 primiparous cows were randomly assigned to treatment in year 1; control (CON, no OG; n = 56 multiparous and 24 primiparous) or treatment (OG fed at 8.8 g/100 kg body weight [BW]; n = 56 multiparous and 24 primiparous). Multiparous cows (mean ± SD = 6.4 ± 0.4 yr; BW = 589 ± 9.2 kg; body condition score [BCS] 6.2 ± 0.07) were used in both years of the experiment and primiparous cows (mean ± SD = 2.1 ± 0.04 yr of age, weighed 400 ± 7.5 kg, and BSC of 5.6 ± 0.06) were only used in the first year of the experiment. CON and OG supplements were offered over two production cycles beginning in December approximately 60 d prior to projected calving through pre-breeding in May of each year. Calves from treatment cows were offered treatments in a creep supplement limited to a daily rate of 1% as-fed of BW prorated for 3-d/wk feeding from mid-July through weaning with OG offered at 8.8 g/100 kg BW. Primiparous cow's BW, BCS, and calf performance were not affected by treatment (P ≥ 0.15) in year 1. BW of multiparous OG cows tended (P = 0.10) to be heavier at weaning in year 1 and was greater (P = 0.05) at the onset of the experiment in year 2. Body condition of OG cows was greater (P ≤ 0.02) at weaning in both years 1 and 2, as well as at the onset of the experiment in year 2. Calves fed OG from the mature cows gained more (P = 0.05) BW during the creep feeding period than CON. Core body temperatures of OG heifers measured during the late summer with intravaginal temperature data loggers tended (P ≤ 0.10) to be less at 1400 and 1700 hours and were less (P = 0.05) at 1800 hours than CON heifers. Feeding OG did not result in changes (P = 0.25) in serum titer response to the BVD virus of calves during year 2. The results of the current experiment indicate feeding OG to beef cows and calves can result in improvement in BCS of cows, enhance weight gain of calves preweaning, and reduce heat loads in heifer calves during the late summer.


Assuntos
Bovinos/fisiologia , Suplementos Nutricionais/análise , Imunidade/efeitos dos fármacos , Fatores Imunológicos/análise , Ração Animal/análise , Animais , Temperatura Corporal , Cruzamento , Bovinos/crescimento & desenvolvimento , Bovinos/imunologia , Dieta/veterinária , Feminino , Masculino , Paridade , Estações do Ano , Estresse Fisiológico/efeitos dos fármacos , Desmame , Aumento de Peso/efeitos dos fármacos
3.
Res Vet Sci ; 127: 11-17, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31670050

RESUMO

Enhancing immunological responses to vaccination is an important goal in many herd health management systems. OmniGen-AF®(OG) is an immunomodulatory feed additive that has been shown to enhance innate immune function in ruminants and its effects on adaptive immunity require additional study. The objective of this study was to evaluate post-vaccine antibody titers and circulating cellular memory development in heifers fed OG and administered a commercially available modified-live bovine respiratory disease (BRD) vaccine. Twenty-four Holstein heifers were assigned to one of two diets for 170 days: Control TMR (CON; n = 11), or TMR plus OG (TRT; 9 g/100 kg BW/day; n = 13). Samples for hematology, serology, and cellular assays were collected on D-110, 0, 21, 42, and 60 of the trial. Heifers were administered two priming doses of a modified-live BRD vaccine, with a third dose given on D0. There were no significant differences in total WBC and absolute number or the percentage of circulating lymphocytes, monocytes, neutrophils, RBC, or platelets on D-110 through D21. On D42 and D60, CON had significantly higher numbers of lymphocytes. On D0, mean serum neutralizing (SN) titer to BHV-1 was significantly higher for CON compared to TRT. SN titers were not significantly different between CON and TRT at any other time point for BHV-1, BVDV type 1, or BVDV type 2. TRT mounted a significantly stronger recall proliferative response to 0.5 multiplicity of infection (MOI) of BHV-1, BVDV type 1 and BVDV type 2 on D42 and D60; 0.25 MOI of BVDV type 1 on D21 and D42; and 0.25 MOI BVDV type 2 on D42 compared to CON. IL-4 production induced by 0.5 and 1.0 MOI BHV-1 (D42 and D60); 0.25 MOI of BVDV type 1 (D21); and 0.25 and 0.5 MOI of BVDV type 2 (D60) were significantly higher for TRT than CON. IL-17 production induced by 0.25 MOI of BVDV type 1 was significantly higher on D60 for TRT compared to CON. IFN-gamma and IL-10 were not significantly different between treatments. These data indicate feeding OG has a beneficial effect on responses to vaccine antigens in Holstein dairy heifers.


Assuntos
Antígenos Virais/imunologia , Vírus da Diarreia Viral Bovina Tipo 1/imunologia , Vírus da Diarreia Viral Bovina Tipo 2/imunologia , Herpesvirus Bovino 1/imunologia , Fatores Imunológicos/imunologia , Vacinas Virais/imunologia , Ração Animal/análise , Animais , Complexo Respiratório Bovino/imunologia , Bovinos , Dieta/veterinária , Suplementos Nutricionais/análise , Feminino , Fatores Imunológicos/administração & dosagem
4.
Res Vet Sci ; 124: 186-190, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30909121

RESUMO

A trial was conducted to determine if feeding OmniGen-AF® (OG) to 22 late lactation cows 60 days prior to and during the early dry period, a time of increased susceptibility to mastitis, could reduce disease incidence in a dairy herd experiencing major health issues. Treated cows (n = 11) consumed a ration containing OG [9 g/100 kg of body weight/day] beginning 60 days before dry-off, during the dry period, and through 30 days in milk (DIM). Control cows received the same ration during the dry period through 30 DIM only. Body weights, body condition scores (BCS), intramammary infection (IMI) prevalence, new IMI rates, somatic cell counts (SCC), milk yield, and adverse health events were measured. No differences were found between treatments for body weight or BCS. Adverse health event data at calving showed no differences between treatments except for percentage of cows with hyperketonemia, which was lower among treated cows (63.6% vs 100%). Prevalence of IMI from calving through 30 DIM for treated cows (6.1%) was lower than controls (11.05%); likewise, new IMI rate during this time for treated cows (0.61%) was lower than controls (5.81%). The SCC from calving through 30 DIM for treated cows (215,000/ml) was lower than controls (493,000/ml). Average production/day at the first DHIA test (~33 DIM) showed that treated cows produced more milk (39.9 kg) than controls (35.34 kg). In conclusion, feeding OG 60 days prior to dry-off reduced hyperketonemia and mastitis, lowered SCC, and numerically increased milk yield in a dairy herd experiencing major health issues.


Assuntos
Suplementos Nutricionais/análise , Glândulas Mamárias Animais/imunologia , Mastite Bovina/prevenção & controle , Leite/metabolismo , Ração Animal/análise , Animais , Bovinos , Contagem de Células/veterinária , Dieta/veterinária , Feminino , Georgia/epidemiologia , Mastite Bovina/epidemiologia , Prevalência
5.
Anim Sci J ; 90(2): 167-177, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30575222

RESUMO

The study investigated the impact of feeding OmniGen-AF® (OG; Phibro Animal Health, Quincy, IL) from dry-off to week 4 of lactation at two doses on production performance and metabolic adaptation of multiparous Holstein cows. Forty-eight cows were blocked and assigned randomly to three treatments: OG was fed at 0 g/head/day (CON), 60 g/head/day (OG60), or 90 g/head/day (OG90). No difference was observed in dry matter intake (DMI) throughout the experiment, whereas feeding OG tended to decrease the percentage body weight change (PWC) on week 2. Although colostrum yield was not affected by treatment, colostrum IgG production of OG90 tended to be higher than that of CON. OG supplementation did not affect overall milking performance but decreased milk SCC during the first 4 weeks of lactation. In prepartum, OG supplementation decreased the concentrations of serum albumin and calcium, and increased serum globulin. OG supplementation tended to increase serum total protein, globulin, and calcium contents postpartum. Furthermore, reduced incidence of mastitis and udder edema were observed. In conclusion, supplementing cows with OG from dry-off period reduces or modulates the inflammation responses associated with parturition, potentially resulting in improved postpartum health, while feeding OG more than 60 g/head/day did not warrant further benefits.


Assuntos
Ração Animal , Peso Corporal , Bovinos/metabolismo , Bovinos/fisiologia , Dieta/veterinária , Aditivos Alimentares , Saúde , Fatores Imunológicos/administração & dosagem , Paridade , Animais , Proteínas Sanguíneas/metabolismo , Cálcio/sangue , Colostro/imunologia , Colostro/metabolismo , Feminino , Imunoglobulina G/metabolismo , Lactação , Parto , Gravidez , Albumina Sérica/metabolismo , Fatores de Tempo
6.
J Dairy Sci ; 100(6): 4829-4838, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28434746

RESUMO

This study compared vaginal temperature, physiologic, and productive parameters in lactating dairy cows supplemented or not with Omnigen-AF (Phibro Animal Health, Teaneck, NJ) during the summer months in a tropical environment. Thirty-two lactating, primiparous (n = 16) and multiparous (n = 16) pregnant Holstein × Gir cows were ranked by parity, days in milk, body weight, and body condition score (BCS), and assigned to receive (SUPP; n = 16) or not (CON; n = 16) Omnigen-AF (Phibro Animal Health, Teaneck, NJ) at 56 g/cow daily (as-fed basis). During the experimental period (d -6 to 56), cows were maintained in a single drylot pen with ad libitum access to water and a total mixed ration, and milked twice daily. Cows received Omnigen-AF mixed with 200 g of corn (as-fed basis) after the daily morning milking through self-locking head gates, whereas CON cows concurrently received 56 g of kaolin mixed with 200 g of corn. For feed intake evaluation, cows from both treatments were randomly divided in 4 groups of 8 cows each, and allocated to 8 individual feeding stations for 3 d. Intake was evaluated 4 times per group from d 1 to 56. From d -6 to 0, d 15 to 28, and d 43 to 56, cow vaginal temperature was recorded hourly. Environmental temperature-humidity index (THI) was also recorded hourly from d 15 to 28 and d 43 to 56. Cows were evaluated for body weight and BCS on d -6 and 56, individual milk production was recorded daily from d -6 to 56, and milk samples were collected on d -6, 0, 7, 14, 21, 28, 35, 42, 49, and 56 for analyses of somatic cell count and milk components. Blood samples were collected on d -6, -3, 0, 9, 15, 18, 21, 24, 27, 36, 45, 48, 51, 54, and 56. Results from samples or observations collected from d -6 to 0 were included as an independent covariate in each respective analysis. Environmental THI was 74.2 ± 0.5 and cows were exposed to THI >68 for 633 h within a total of 672 h of evaluation. Cows assigned to CON had greater vaginal temperature on d 28, 43, 45, and from d 48 to 55 (by 0.38 to 0.52%), as well as greater mean somatic cell count (by 97%) and serum haptoglobin concentrations (by 89%) compared with SUPP cows. Cows assigned to SUPP had greater mean dry matter intake (by 7%), BCS on d 56 (by 11%), and mean serum insulin concentrations (by 35%) compared with CON cows. Hence, SUPP ameliorated hyperthermia, improved nutritional status, and modulated systemic and mammary gland immune parameters in lactating dairy cows exposed to heat stress conditions.


Assuntos
Ração Animal , Regulação da Temperatura Corporal/fisiologia , Doenças dos Bovinos/fisiopatologia , Transtornos de Estresse por Calor/veterinária , Imunomodulação , Lactação/fisiologia , Animais , Peso Corporal , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/imunologia , Dieta , Suplementos Nutricionais , Feminino , Transtornos de Estresse por Calor/sangue , Transtornos de Estresse por Calor/imunologia , Transtornos de Estresse por Calor/fisiopatologia , Temperatura Alta/efeitos adversos , Umidade , Lactação/imunologia , Leite/metabolismo , Paridade , Gravidez , Vagina/fisiopatologia
7.
J Dairy Sci ; 99(7): 5562-5572, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27085398

RESUMO

This study compared physiological, health, and productive parameters in dairy cows supplemented or not with Omnigen-AF (OMN; Phibro Animal Health, Teaneck, NJ) during the transition period. Thirty-eight nonlactating, multiparous, pregnant Holstein × Gir cows were ranked by body weight (BW) and body condition score (BCS), and assigned to receive (n=19) or not (CON; n=19) OMN at 56 g/cow daily (as-fed basis) beginning 35 d before expected date of calving. Before calving, cows were maintained in single drylot pen with ad libitum access to corn silage, and received (as-fed basis) 3kg/cow daily of a concentrate. After calving, cows were moved to an adjacent drylot pen, milked twice daily, offered (as-fed basis) 35kg/cow daily of corn silage, and individually received a concentrate formulated to meet their nutritional requirements after both milkings. Cows received OMN individually as top-dressing in the morning concentrate feeding. Before calving, cow BW and BCS were recorded weekly and blood samples were collected every 5 d beginning on d -35 relative to expected calving date. After calving and until 46 d in milk, BW and BCS were recorded weekly, individual milk production was recorded, and milk samples were collected daily for total solids and somatic cell count analyses. Blood was sampled daily from 0 to 7 d in milk, every other day from 9 to 21 d in milk, and every 5 d from 26 to 46 d in milk. On 30 and 46 d in milk, cows were evaluated for endometritis via cytobrush technique, based on % of polymorphonuclear (PMN) cells in 100 total cell count (PMN + endometrial cells). On 48.7±1.6 d in milk, 9 cows/treatment received a lipopolysaccharide (LPS) injection (0.25µg/kg of BW), and blood was sampled hourly from -2 to 8 h, at 12-h intervals from 12 to 72 h, and at 24-h intervals form 96 to 120 h relative to LPS administration. No treatment differences were detected on BW, BCS, serum concentrations of cortisol, fatty acids, insulin, glucose, haptoglobin, cortisol, and insulin-like growth factor-I. Cows receiving OMN had greater milk yield (30.3 vs. 27.1kg/d) and percentage of PMN cells in endometrial cell population (12.2 vs. 3.9%) compared with CON cows. After LPS administration, cows receiving OMN had greater mean serum haptoglobin (212 vs. 94 µg/mL), as well as greater serum concentration of tumor necrosis factor α at 1, 2, and 3 h relative to LPS injection compared with CON cows. In conclusion, OMN supplementation during the transition period enhanced innate immunity parameters and increased milk production in dairy cows.


Assuntos
Suplementos Nutricionais , Lactação , Ração Animal , Animais , Bovinos , Dieta/veterinária , Feminino , Leite , Paridade , Silagem
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