Trace element levels in the muscles of three tern species (Aves: Laridae) from the western Arabian Gulf: environmental assessment and implications for conservation.

In the Arabian Gulf (called also Persian Gulf; hereafter 'the Gulf'), Jana and Karan Islands are recognized as one of the most Important Bird Areas in the region. Many migratory breeding seabirds, like the Greater Crested Tern Thalasseus bergii, White-cheeked Tern Sterna repressa and Bridled Tern Onychoprion anaethetus, depend on these islands during the breeding season. However, these aquatic wildlife species are suffering from intensified urban and industrial coastal development and various contamination events including wars and related oil spills. In this study, we used these three piscivorous top predator birds to analyse the levels of 19 trace elements (TEs; i.e. Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Na, Ni, Pb, Sr, V and Zn) in 15 muscular tissue samples from Jana and Karan Islands. PERMANOVA analysis showed no difference in contamination profile between sites nor between species probably due to their spatial and ecological proximity and therefore similar levels of exposure to TEs. Comparing these levels with existing literature, our results showed no particular concern for all elements, except for Al (maximum values recorded = 116.5 µg g-1 d.w.) and, in two samples, Ba (33.67 µg g-1 d.w.) and Pb (5.6 µg g-1 d.w.). The results can be considered as an initial step for supplementary evaluations with a larger number of samples and specified time intervals for the collection of specimens. This study provided baseline information on the pollution status of these two ecologically important sites which require a continuous biomonitoring programme.

Comparison of Cd, Cu, Se, and Zn Concentration in the Muscle and Hepatopancreas of Sepia pharaonis and Uroteuthis duvauceli in the North of Persian Gulf (Iran).

The objective of this research was to examine and contrast the levels of cadmium (Cd), copper (Cu), zinc (Zn), and selenium (Se) in the muscle and hepatopancreas tissues of two species, namely pharaoh cuttlefish (Sepia pharaonis) and Indian squid (Uroteuthis duvauceli), from the Persian Gulf. A total of thirty individuals of each species were gathered in January 2009 from the northern waters of the Persian Gulf. The metal concentrations were significantly higher in muscle tissue (p < 0.05) than in other tissues. S. pharaonis had higher metal concentrations than U. duvauceli. In the muscle and hepatopancreas samples of S. pharaonis, the highest mean concentrations were found to be for Zn (58.45 ± 0.96 µg/g dw) and Cu (1541.47 ± 192.15 µg/g dw), respectively. In U. duvauceli, the highest concentration of measured elements was seen for Zn in both muscle (36.52 ± 0.56 µg/g dw) and hepatopancreas (60.94 ± 2.65 µg/g dw). Se had the lowest concentration among the elements measured in both species. There was a negative and significant correlation between Cu and biometrical factors (total body length and weight) in both muscle and hepatopancreas samples of S. pharaonic and only in the muscle samples of U. duvauceli (p < 0.01, R2 = - 052; p < 0.01, R2 = - 0.055). However, there was a strong correlation between Zn and biometrical factors in hepatopancreas samples of both species. The comparison of metal concentrations with standards revealed that only Cd levels in S. pharaonis exceeded the ESFA and WHO standards, whereas other metals were below the standards.

Self-reported gastrointestinal disorders among veterans with gulf war illness with and without posttraumatic stress disorder.

BACKGROUND: Gulf War Illness (GWI) is a chronic, multi-symptom disorder affecting 25%-32% of Gulf War veterans. Veterans with GWI disproportionately suffer from gastrointestinal (GI) disorders. Given the increasing evidence supporting a gut-brain axis, we explore the relationship between post-traumatic stress disorder (PTSD), GWI, and self-reported GI disorders among GW veterans. METHODS: Veterans from the Gulf War Era Cohort and Biorepository responded to a mail-based survey (N = 1058). They were stratified by GWI (Centers for Disease Control definition) and PTSD status. This yielded three groups: GWI-, GWI+/PTSD-, and GWI+/PTSD+. Multivariable logistic regression adjusting for demographic and military characteristics examined associations between GWI/PTSD groups and GI disorders. Results were expressed as adjusted odds ratios (aOR) with 95% confidence intervals (95% CI). KEY RESULTS: The most frequently reported GI disorders were irritable bowel syndrome (IBS), gastroesophageal reflux disease (GERD), and colon polyps (CP). The GWI+/PTSD+ group had a higher odds of these disorders than the GWI+/PTSD- group (aORIBS  = 3.12, 95% CI: 1.93-5.05; aORGERD  = 2.04, 95% CI: 1.44-2.90; aORCP  = 1.85, 95% CI: 1.23-2.80), which had a higher odds of these disorders than the GWI- group (aORIBS  = 4.38, 95% CI: 1.55-12.36; aORGERD  = 2.51 95% CI: 1.63-3.87; aORCP  = 2.57, 95% CI: 1.53-4.32). CONCLUSIONS & INFERENCES: GW veterans with GWI and PTSD have significantly higher odds of specific self-reported GI disorders than the other groups. Given the known bidirectional influences of the gut and brain, these veterans may benefit from a holistic healthcare approach that considers biopsychosocial contributors to the assessment and management of disease.

Exploring in vitro effect of silver nanoparticles and Holothuria parva extracts on kinetics and stability of α-amylase.

Diabetes is a chronic metabolic disorder characterized by elevated blood glucose levels. Major limitations of synthetic drugs, including high cost, efficacy, and adverse side effects, have prompted researchers to seek more effective and low-cost alternative therapies with fewer adverse effects. Marine life forms are considered the most important sources of biologically active natural products due to their secondary metabolites. In this research, sea cucumber Holothuria parva was collected from coastal areas of Bandar Lengeh, Hormozgan, Iran, and was then subjected to extraction. The results showed that compounds extracted from Holothuria parva had a stimulatory effect on enzyme activity, and in the presence of these compounds, the Vmax value of the enzyme was increased about two times, while the Km value was reduced. The phosphate buffer form of extracts had the greatest impact on enzyme activity. Upon an increase in the concentration of silver nanoparticles (AgNPs), the α-amylase activity was inhibited in parallel. Silver nanoparticles exhibited the highest enzyme inhibition with an IC50 of 0.86 mg/ml. Silver nanoparticles showed anti-α-amylase activity and had the ability to decrease intestinal glucose uptake in diabetic individuals when prescribed as a novel supplementary medicine.

Persistent organic pollutants influence the marine benthic macroinvertebrate assemblages in surface sediments of Nayband National Park and Bay, Northern Persian Gulf, Iran.

Macroinvertebrate communities have been influenced by chemical substances, originated from petrochemical developments, that caused many problems in the marine biota. This study investigated the surface sediments of Nayband National Park and Bay (northern Persian Gulf) for polycyclic aromatic hydrocarbons (PAHs) and total petroleum hydrocarbons (TPHs) in terms of their distribution, source, and impacts on benthic macroinvertebrate assemblages. To this end, a total of 180 surface sediment samples from 20 stations were collected using Van-Veen grab sampler during winter 2018. The concentration of PAHs, TPHs, total organic carbon (TOC). and total organic matter (TOM) were evaluated, and grain size measurements were conducted on sediment samples in this study. Benthic macroinvertebrates were then identified in terms of presence and distribution. The results indicated that coarse granulometric fractions of sands were prevalence in all samples stations. The total concentration of PAHs ranged from 47.57 to 657.68 ng/g and TPHs 5.72 to 42.16 µg/g dw. The risk of ΣPAHs and TPHs in the sediments was relatively low to moderate according to the sediment quality guidelines. Analysis of the results revealed a significant negative correlation between ΣPAHs (R-value = - 0.917; P < 0.01), TPHs (R-value = - 0.849; P < 0.01) and macrofaunal abundance. Findings demonstrated that the species richness and abundance were at the lowest levels in stations where concentrations of PAHs, TPHs, TOC, and TOM were in the highest values, suggesting that these contaminants could negatively influence the benthic organisms in Nayband National Park and Bay. The results of correspondence analysis (CA) and principal component analysis (PCA) analysis showed that sedimentary habitats in Nayband National Park and Bay are being negatively affected by PAHs and TPHs, released from Pars Special Economic Energy Zone (PSEEZ). Moreover, the marine biotic index (AMBI) and Shannon-Weiner Diversity (H') results suggest that Nayband National Park and Bay can be classified as slightly to moderate polluted area. In conclusion, Northern Persian Gulf is significantly affected by oil industry developments and petrochemical activities. The unique ecosystem like Nayband National Park and Bay has been in a cautious status in terms of the PSEEZ pollutants and the levels of PAHs and TPHs concentration, warning that urgent environmental programs should be considered to protect the diversity and ecology of this valuable marine systems.

Molecular Networking-Guided Isolation of New Etzionin-Type Diketopiperazine Hydroxamates from the Persian Gulf Sponge Cliona celata.

The Persian Gulf is a unique and biologically diverse marine environment dominated by invertebrates. In continuation of our research interest in the chemistry and biological activity of marine sponges from the Persian Gulf, we selected the excavating sponge Cliona celata for detailed metabolome analyses, in vitro bioactivity screening, and chemical isolation studies. A UPLC-MS/MS (MS2) molecular-networking-based dereplication strategy allowed annotation and structural prediction of various diketopiperazines (DKPs) and etzionin-type diketopiperazine hydroxamates (DKPHs) in the crude sponge extract. The molecular-networking-guided isolation approach applied to the crude extract afforded the DKPH etzionin (1) and its two new derivatives, clioetzionin A (2) and clioetzionin B (3). Another new modified DKP (4) was identified by MS/MS analyses but could not be isolated in sufficient quantities to confirm its structure. The chemical characterization of the purified DKPHs 1-3 was performed by a combination of 1D and 2D NMR spectroscopy, HRMS, HRMS/MS, and [α]D analyses. Compounds 1 and 2 exhibited broad antibacterial, antifungal, and anticancer activities, with IC50 values ranging from 19.6 to 159.1 µM. This is the first study investigating the chemical constituents of a C. celata specimen from the Persian Gulf. It is also the first report of full spectroscopic data of etzionin based on extensive spectroscopic analyses.

Normal alkanes in sediments from the Persian Gulf: spatial pattern and implications for autochthonous, allochthonous, and petroleum-originated contaminants.

Surface sediments (n = 124) were gathered to evaluate spatial pattern and source apportionment of ∑24n-alkanes in the Persian Gulf. Moran's I indices revealed that ∑24n-alkanes were randomly distributed in sampling space in general. One noticeable exception was a significant spatial high-high cluster (Z-score = 8.6). Profile of n-alkanes observed for this cluster maximized at n-C20, n-C18, n-C16, and n-C22. Detection of very low carbon preference indices and strong even carbon-numbered predominance suggested that bacterial inputs were the process making this cluster. Based on diagnostic ratios and GIS techniques, in situ production of organic materials was widespread in the studied space. Allochthonous and petroleum-based inputs were not traceable for 73.8% and 24.7% of the area, respectively. Petrogenic source apportionment map proposed that petroleum-originated contaminants were probably limited to 6.32 km2 of the sampling matrix. The biogenic inputs (autochthonous and allochthonous inputs) and not the petroleum contaminants were the main source of n-alkanes deposited.

Evaluation of the Bioactive Potential of Secondary Metabolites Produced by a New Marine Micrococcus Species Isolated from the Persian Gulf.

BACKGROUND: In the present work, a newly isolated marine bacterium, Micrococcus sp. MP76, from coastal area of Persian Gulf around Bushehr province, Iran, was identified with the ability to produce bioactive compounds. METHODS: The pigment production was optimized by changing carbon and nitrogen sources in bacterial growth media at 28°C and 220 rpm for 5 days. Partial purification of the pigment was carried out using suitable solvents. RESULTS: Maximum pigment extract was achieved (1.4 g/l) when cultured in the medium containing 0.5% (v/v) molasses, 0.5% (w/v) peptone, 1% (w/v) sea salt, 0.01% (w/v) potassium phosphate, and 0.05% (w/v) yeast extract, pH=7.0. Antibacterial effect assessment of the extract against pathogenic bacteria revealed the MIC values in the range of 4.2-7.5 mg/ml depending on different pathogens. The pigment extracted from medium supplemented by molasses and ammonium sulfate had 81% radical scavenging activity, and its IC50 value was 0.28 mg/ml. CONCLUSION: The newly isolated strain of Micrococcus genus from the Persian Gulf revealed a valuable source to access worth medicinal ingredients when cultured under optimized conditions.

Phytochemical analysis of Holothuria leucospilota, a sea cucumber from Persian Gulf.

Sea cucumbers are widely consumed in traditional medicine and food. These animals have a considerable secondary metabolite and also several potential biological activities. This study investigated the phytochemical and cytotoxic evaluation of Holothuria leucospilota (H. leucospilota), a sea cucumber from Persian Gulf. The saponin composition of H. leucospilota was studied by different partitioning and chromatography methods such as thin layer chromatography (TLC), medium pressure liquid chromatography (MPLC) and high performance LC (HPLC). The marine sea cucumber Holostane-type triterpenoids (1-3) were characterized by physical and spectroscopic examination (1 and 2 dimensional neuclear magnetic resonance and mass experiments) with data analysis. The structure of compounds 1-3 identified as echinoside A, holothurine A, and 24-dehydroechinoside A, showed moderate cytotoxic activity with IC50 values of 1.9 ± 0.07, 6.8 ± 0.23, and 2.57 ± 0.18 µg/mL against HeLa and 10.4 ± 0.32, 8.9 ± 0.24, and 4.4 ± 0.13 on HUVEC cell line, respectively. In conclusion, the holostane-type triterpenoids showed moderate cytotoxic activity against HeLa cell line and have a prosperous future to be introduced as a lead structure.

The effect of different partitions of seaweed Sargassum plagyophylum on depression behavior in mice model of despair.

Background Seaweeds are a famous traditional food resource in some countries containing different types of secondary metabolites. These marine organisms have shown different biological activities. The aim of this study was to investigate the effects of hexane and methanol extracts of Sargassum plagyophylum on depression. Methods Sargassum plagyophylum was collected from Persian Gulf. The plant was extracted by maceration with methanol-ethyl acetate solvent. The extract was evaporated and partitioned by hexane and methanol solvents. The two partitions were administered i.p. to male mice either a single dose or for 7 days. Depression was evaluated by the forced swimming test (FST) which higher immobility time indicates depressive-like behavior. Results The immobility time during FST decreased significantly by all the doses of the hexane partitions (notably 40 mg/kg; 10 s ± 2 vs. 114 s ± 12 control group). However, only the lowest dose (20 mg/kg) of the methanol partition reduced immobility time during FST (23 s ± 8, p<0.001). Following the long term administration both of the partitions reduced the immobility time in FST (hexane 27 s ± 11, methanol 70 s ± 14, p<0.05 vs. control 140 s ± 14). Conclusion The hexane partition showed antidepressant effects not only by long-term administration but also by the single dose during FST. The 7 days therapy with methanol partition also induced antidepressant behavior, but only the lowest single dose reduced immobility in FST. The methanol partitions possibly have certain substance that interfered with behavior in the FST. Therefore, S. plagyophylum should be considered for further antidepressant studies.

Assessment of the toxicity, origin, biodegradation and weathering extent of petroleum hydrocarbons in surface sediments of Pars Special Economic Energy Zone, Persian Gulf.

During the last years, the oil and gas explorations, extractions and refineries have led to severe ecological damages into the sensitive environment of the Persian Gulf. In this study, the level of oil contamination, the source of hydrocarbons and the degree of weathering or degradation extent were investigated in the Surface Sediments of Pars Special Zone, Persian Gulf. Fifteen sediment samples were collected. The Gas Chromatography-Mass Spectrometry used to analyze Aliphatic Hydrocarbons (AHC) and EPA's sixteen compounds of Polycyclic Aromatic Hydrocarbons (PAHs). The total concentration of Aliphatic Hydrocarbons found to be in the range of 693 to 3752 µg/g and the 16 EPA PAHs compounds varied between 46.6 and 84.7 ng/g dry weight in the region. The concentration of hydrocarbons found to be lower than the level of threshold effects. The source of compounds was also identified by developing the multiple indices.

The comparison of selenium and lead accumulation between contaminated muddy and sandy sediments from four estuaries along the Persian Gulf: effect of grain size.

Persian Gulf is one of the most important water sources in the economically developed south part of Iran, and metal pollution is a major concern for the Gulf. The bioavailability and distribution of selenium (Se) and lead (Pb) between muddy and sandy sediments from four estuaries along the Persian Gulf were analyzed. The geoaccumulation index and enrichment factor for metals and correlation between particles size with metals concentration were studied. The average concentration of metals in sediment was ranged 0.08-1.14 µg/g for Se and 0.32-4.37 µg/g for Pb in all estuaries, with the highest concentrations in Musa estuary. The results showed there was positive correlation between particles size of sediment with metals concentration. The highest of metal concentration was absorbed in silt (< 63 µm) sediment, followed by extremely fine sand (63-125 µm), fine sand (125-250 µm), medium sand (250-500 µm) and coarse sand (500-1000 µm), respectively. The organic matter and carbonate in the muddy sediment are higher than sandy sediment, and they had high specific storage capacity for accumulation of heavy metals. The result of Pearson correlation (r) for organic matter and metal was 0.78 for Se and 0.67 for Pb, and for carbonates and metal was 0.54 for Se and 0.61 for Pb. The values of EF in all estuaries show that the enrichment of sediment by heavy metals was by anthropogenic activities such as discharge of petrochemical and oil industrial. Geoaccumulation index indicates that the sediment in the Ahmadi and Ghanam estuaries was unpolluted to moderately polluted, while in the Arvand river is moderately to strongly polluted, and in the Musa estuary is extremely polluted.

Depleted Uranium and Human Health.

Depleted uranium (DU) is generally considered an emerging pollutant, first extensively introduced into environment in the early nineties in Iraq, during the military operation called "Desert Storm". DU has been hypothesized to represent a hazardous element both for soldiers exposed as well as for the inhabitants of the polluted areas in the war zones. In this review, the possible consequences on human health of DU released in the environment are critically analyzed. In the first part, the chemical properties of DU and the principal civil and military uses are summarized. A concise analysis of the mechanisms underlying absorption, blood transport, tissue distribution and excretion of DU in the human body is the subject of the second part of this article. The following sections deal with pathological condition putatively associated with overexposure to DU. Developmental and birth defects, the Persian Gulf syndrome, and kidney diseases that have been associated to DU are the arguments treated in the third section. Finally, data regarding DU exposure and cancer insurgence will be critically analyzed, including leukemia/lymphoma, lung cancer, uterine cervix cancer, breast cancer, bladder cancer and testicular cancer. The aim of the authors is to give a contribution to the debate on DU and its effects on human health and disease.

Spatial distribution and composition of aliphatic hydrocarbons, polycyclic aromatic hydrocarbons and hopanes in superficial sediments of the coral reefs of the Persian Gulf, Iran.

This study is the first quantitative report on petroleum biomarkers from the coral reefs systems of the Persian Gulf. 120 reef surface sediment samples from ten fragile coral reef ecosystems were collected and analyzed for grain size, biogenic elements, elemental ratios, and petroleum biomarkers (n-alkanes, PAHs1 and Hopanes) to assess the sources and early diagenesis of sedimentary organic matter. The mean grain size of the reef sediments ranged from 13.56 to 37.11% (Clay), 26.92 to 51.73% (Sand) and 35.97 to 43.85% (Silt). TOC2 (3.35-9.72 mg.g-1) and TON3 (0.4-1.10 mg.g-1) were identified as influencing factors on the accumulation of petroleum hydrocarbons, whilst BC4 (1.08-3.28 mg.g-1) and TIN5 (0.13-0.86) did not exhibit any determining effect. Although BC and TIN demonstrated heterogeneous spatial distribution, TOC and TON indicated homogenous distribution with continually upward trend in concentration from the east to west ward of the Gulf. The mean calculated TOC/TN ratios vacillated according to the stations (p < 0.05) from 2.96 at Shidvar Island to 8.64 at Hengam Island. The high TOC/TN ratios were observed in the Hengam (8.64), Kharg (8.04) and Siri (6.29), respectively, suggesting a predominant marine origin. The mean concentrations of ∑C11-35n-alkanes, ∑30 PAHs and ∑9Hopanes were found in the ranges of 385-937 µg.g-1dw, (overall mean:590 µg.g-1dw), 326-793 ng.g-1dw (499 ng.g-1dw), 88 to 568 ng.g-1 d (258 ng.g-1dw), respectively. Higher concentrations of detected petroleum biomarkers in reef sediments were chiefly distributed near main industrial areas, Kharg, Lavan and Siri, whilst the lower concentrations were in Hormoz and Qeshm. In addition, one-way ANOVA6 analysis demonstrated considerably significant differences (p < 0.05) among concentration of detected total petroleum hydrocarbons between most sampling locations. Some sampling sites especially Kharg, Lavan, Siri and Lark indicated higher concentration of n-alkanes due to the higher maintenance of organic matter by high clay content in the sediments. Furthermore, most sediment samples, except for Hormoz, Qeshm and Hengam showed an even carbon preference for n-alkanes which could be correlated to bacterial input. NPMDS7 analysis also demonstrated that among the congeners of petroleum biomarkers, n-C12,n-C14, n-C16,n-C18 and n-C20 for n-alkanes, Phe8 and Naph9 along with their Alkyl homologues for PAHs (2-3 rings accounted for 60%) and C30αß and C29αß for Hopanes were discriminated from their other congeners in the whole study area. Our results based on the PCA10 analysis and diagnostic indices of AHs11 and PAHs along with ring classification of PAHs, in addition, the ubiquitous presence of UCM,12 and Hopanes revealed that the main sources of the pollution were petroleum and petroleum combustion mainly from offshore oil exploration and extraction, discharge of pollutants from shipping activities.

Study the symbiotic crude oil-degrading bacteria in the mussel Mactra stultorum collected from the Persian Gulf.

Symbiotic associations are complex partnerships that can lead to new metabolic capabilities and the establishment of novel organisms. The diversity of these associations is very broad and there are still many mysteries about the origin and the exact relationship between the organisms that are involved in a symbiosis. The aim of the present study is to find symbiotic crude-oil degrading bacteria in the mussels that collected from the Persian Gulf. Fifteen crude-oil degrading bacteria were isolated from Mactra stultorum mussel that collected from oil contaminated area at Persian Gulf. According to high growth rate on crude oil five strains were selected from 15 isolated strains for more study. Determination of the nucleotide sequence of the gene encoding 16S rRNA show that these isolated strains belong to: Alcanivorax dieselolei strain BHA25, Idiomarina baltica strain BHA28, A. dieselolei strain BHA30, Alcanivorax sp. strain BHA32 and Vibrio azureus strain BHA36. Analysis of remaining of crude oil by Gas Chromatography (GC) confirmed that these strains can degrade: 64%, 63%, 71%, 58% and 75% of crude oil respectively.

Enrichment and isolation of crude oil degrading bacteria from some mussels collected from the Persian Gulf.

To date, little is known about existing relationships between mussels and bacteria in hydrocarbon-contaminated marine environments. The aim of this study is to find crude oil degrading bacteria in some mussels at the Persian Gulf. Twenty eight crude oil degrading bacteria were isolated from three mussels species collected from oil contaminated area at Persian Gulf. According to high growth and degradation of crude oil four strains were selected between 28 isolated strains for more study. Determination the nucleotide sequence of the gene encoding for 16S rRNA show that these isolated strains belong to: Shewanella algae isolate BHA1, Micrococcus luteus isolate BHA7, Pseudoalteromonas sp. isolate BHA8 and Shewanella haliotis isolate BHA35. The residual crude oil in culture medium was analysis by Gas Chromatography (GC). The results confirmed that these strains can degrade: 47.24%, 66.08%, 27.13% and 69.17% of crude oil respectively. These strains had high emulsification activity and biosurfactant production. Also, the effects of some factors on crude oil degradation by isolated strains were studied. The results show that the optimum concentration of crude oil was 2.5% and the best degradation take place at 12% of salinity. This research is the first reports on characterization of crude oil degrading bacteria from mussels at Persian Gulf and by using of these bacteria in the field the effect of oil pollution can be reduce on this marine environment.

Concentrations of trace elements in a rare and threatened coastal shark from the Arabian Gulf (smoothtooth blacktip Carcharhinus leiodon).

Kuwait's waters are one of only two locations where the smoothtooth blacktip shark Carcharhinus leiodon is known to occur. Concentrations of 11 trace elements were analysed in five juvenile and two adult specimens of this coastal predator. Concentrations of lead in muscle increased with length, whilst manganese concentration decreased. Arsenic concentrations in muscle were among the highest reported in elasmobranchs, and the concentration in the liver increased significantly in relation to length. In comparison to published literature, concentrations of manganese (liver), lead (muscle) and iron (muscle and liver) were high. Mercury concentrations in the muscle exceeded European Food Safety Authority limits and were among the highest reported in any elasmobranch. Concentrations of selenium, which may inhibit mercury toxicity, were also high. These results and previous studies indicate that potentially hazardous levels of mercury and other contaminants may occur in sharks in this region, adding further stressors to these vulnerable populations.

Total petroleum hydrocarbons in sediments from the coastline and mangroves of the northern Persian Gulf.

To provide baseline information for the marine ecosystem of Hormozgan province, the distribution of petroleum hydrocarbons was evaluated in 52 stations involved in the mangrove and coastline ecosystem. Coastline sampling sites included areas facing harbor, river, domestic and industrial discharge. Sediment samples were analyzed based on ultraviolet fluorescence spectroscopy. Petroleum hydrocarbons showed narrow variations ranging from non-detectable (ND) to 1.71 and from 0.2 to 0.63µg/g dry weight for coastline and mangrove sediments, respectively. The detected concentrations for total petroleum hydrocarbons were lower than guideline values for ecological risk. Furthermore, the minimum environmental risk was confirmed by background levels for the Persian Gulf, the Sea of Oman, and detected values for reference areas. The results were regarded as background data in the studied area, and, considering the rapid expansion of activities related to the petroleum industry in Hormozgan province, the continuous monitoring of pollutants is recommended.

Isolation and characterization of biosurfactant producing bacteria from Persian Gulf (Bushehr provenance).

Biosurfactants are surface active materials that are produced by some microorganisms. These molecules increase biodegradation of insoluble pollutants. In this study sediments and seawater samples were collected from the coastline of Bushehr provenance in the Persian Gulf and their biosurfactant producing bacteria were isolated. Biosurfactant producing bacteria were isolated by using an enrichment method in Bushnell-Hass medium with diesel oil as the sole carbon source. Five screening tests were used for selection of Biosurfactant producing bacteria: hemolysis in blood agar, oil spreading, drop collapse, emulsification activity and Bacterial Adhesion to Hydrocarbon test (BATH). These bacteria were identified using biochemical and molecular methods. Eighty different colonies were isolated from the collected samples. The most biosurfactant producing isolates related to petrochemical plants of Khark Island. Fourteen biosurfactant producing bacteria were selected between these isolates and 7 isolates were screened as these were predominant producers that belong to Shewanella alga, Shewanella upenei, Vibrio furnissii, Gallaecimonas pentaromativorans, Brevibacterium epidermidis, Psychrobacter namhaensis and Pseudomonas fluorescens. The largest clear zone diameters in oil spreading were observed for G. pentaromativorans strain O15. Also, this strain has the best emulsification activity and reduction of surface tension, suggesting it is the best of thee isolated strains. The results of this study confirmed that there is high diversity of biosurfactant producing bacteria in marine ecosystem of Iran and by application of these bacteria in petrochemical waste water environmental problems can be assisted.

Evaluation of antibacterial, antifungal and cytotoxic effects of Holothuria scabra from the North Coast of the Persian Gulf.

Bioactive compounds of the gonad, respiration tree, and body wall of the sea cucumber Holothuria scabra collected from the North Coast of the Persian Gulf were extracted using ethyl acetate, methanol and water-methanol mixtures. Extracts were evaluated for their antibacterial and antifungal activities against Aspergillus niger, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The activity was determined using the disc diffusion test. Cytotoxic activities of the extracts were determined by brine shrimp lethality assay. Results showed the existence of an antifungal activity of all extracts against A. niger with MIC ranging from 3 to 9µg/mL. The highest antifungal activity was found in respiration tree (methanol) with an inhibition zone of about 50mm against A. niger at 18µg/mL extract concentration. Cytotoxic activity was obtained for methanolic extracts only. Methanol extract of the gonads showed the highest cytotoxic effect (LC50=50.5µg/mL) continuing with RT methanol extract (LC50=70µg/mL).