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1.
PLoS One ; 14(5): e0217365, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31136609

RESUMO

Plastic polymers can be combined with additives that modify physical properties and stability of the material. However, the biocompatibility of those additives is not well known. The objective of the study was to characterize the impact of zinc stearate-a common additive-through the development of a novel three-dimensional (3-D) in vitro platform with endometrial cells from domestic cats. Epithelial and stromal cells from adult uteri were isolated and cultured in medium supplemented with 3% Matrigel for two weeks in plastic tissue culture dishes that had been identified as polystyrene with and without zinc stearate by Raman, FTIR, and X-ray fluorescence spectroscopies. Three-dimensional cell structures that were obtained were measured and categorized by shape. Cell viability, proliferation, differentiation, organization, and apoptosis then were assessed by immuno-staining. Results indicated that zinc stearate did not affect 3-D endometrial cell structure morphology, viability, or cellular composition. This first study of a new in vitro platform will be useful for studies testing the influence of other additives, drugs, or exogenous hormones.


Assuntos
Técnicas de Cultura de Células/métodos , Endométrio/citologia , Plásticos/toxicidade , Animais , Apoptose/efeitos dos fármacos , Gatos , Técnicas de Cultura de Células/instrumentação , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Feminino , Organoides/citologia , Organoides/efeitos dos fármacos , Poliestirenos/toxicidade , Ácidos Esteáricos/toxicidade , Células Estromais/citologia , Células Estromais/efeitos dos fármacos
2.
Int J Pharm ; 404(1-2): 211-9, 2011 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-21075187

RESUMO

Nanoparticle-mediated drug delivery represents the future in terms of treating inner ear diseases. Lipid core nanocapsules (LNCs), 50 nm in size, were shown to pass though the round window membrane (RWM) and reached the spiral ganglion cells and nerve fibers, among other cell types in the inner ear. The present study aimed to evaluate the toxicity of the LNCs in vitro and in vivo, utilizing intact round window membrane delivery in rats. The primary cochlear cells and mouse fibroblast cells treated with LNCs displayed dosage dependant toxicity. In vivo study showed that administration of LNCs did not cause hearing loss, nanoparticle application-related cell death, or morphological changes in the inner ear, at up to 28 days of observation. The cochlear neural elements, such as synaptophysin, ribbon synapses, and S-100, were not affected by the administration of LNCs. However, expression of neurofilament-200 decreased in SGCs and in cochlear nerve in osseous spiral lamina canal after LNC delivery, a phenomenon that requires further investigation. LNCs are potential vectors for the delivery of drugs to the inner ear.


Assuntos
Materiais Biocompatíveis , Portadores de Fármacos , Lipídeos/toxicidade , Nanocápsulas , Janela da Cóclea/efeitos dos fármacos , Estimulação Acústica , Animais , Animais Recém-Nascidos , Limiar Auditivo/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Química Farmacêutica , Relação Dose-Resposta a Droga , Composição de Medicamentos , Potenciais Evocados Auditivos do Tronco Encefálico/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Concentração Inibidora 50 , Lipídeos/química , Masculino , Camundongos , Nanotecnologia , Lectinas de Plantas/química , Lectinas de Plantas/toxicidade , Polietilenoglicóis/química , Polietilenoglicóis/toxicidade , Ratos , Ratos Sprague-Dawley , Janela da Cóclea/metabolismo , Janela da Cóclea/patologia , Proteínas de Soja/química , Proteínas de Soja/toxicidade , Estearatos/química , Estearatos/toxicidade , Ácidos Esteáricos/química , Ácidos Esteáricos/toxicidade , Tecnologia Farmacêutica/métodos , Triglicerídeos/química , Triglicerídeos/toxicidade
3.
J Pharm Sci ; 100(1): 242-51, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20575052

RESUMO

The potential use of nanostructured lipid carriers (NLC) composed of a fatty acid [stearic acid (SA)] or a triglyceride (glyceryl behenate) as solid lipids, and a mixture of medium chain triglycerides and castor oil as liquid lipids, for skin administration of flurbiprofen (FB), has been explored. Two different optimized NLC formulations (FB-SANLC based on SA vs. FB-C888NLC based on glyceryl behenate), with respect to the morphometrical properties (particle size and polydispersity index) and the entrapment efficiency, were used in this study. The ex vivo permeation profiles of FB-C888NLC, FB-SANLC and conventional FB solution were evaluated using human skin. An improved FB permeation was observed when the drug was delivered by skin application of FB-C888NLC, attributed to the particle size and matrix crystallinity. The differential scanning calorimetry and X-ray diffraction studies suggested major polymorphic transitions in the lipid matrix of FB-C888NLC. A good correlation between polymorphic transitions and increased drug permeation was observed. However, both NLC dispersions showed a penetration-enhancing ratio (ER) higher than conventional FB solution. The in vitro and in vivo irritancy and local tolerability were assessed by running, respectively, the SKINTEX™ and Draize test. Both FB-C888NLC and FB-SANLC were classified as nonirritant.


Assuntos
Anti-Inflamatórios não Esteroides/administração & dosagem , Portadores de Fármacos/administração & dosagem , Flurbiprofeno/administração & dosagem , Lipídeos/química , Nanoestruturas/química , Pele/metabolismo , Adulto , Animais , Anti-Inflamatórios não Esteroides/farmacocinética , Anti-Inflamatórios não Esteroides/toxicidade , Óleo de Rícino/química , Óleo de Rícino/toxicidade , Portadores de Fármacos/farmacocinética , Portadores de Fármacos/toxicidade , Composição de Medicamentos , Ácidos Graxos/química , Ácidos Graxos/toxicidade , Feminino , Flurbiprofeno/farmacocinética , Flurbiprofeno/toxicidade , Humanos , Técnicas In Vitro , Lipídeos/toxicidade , Masculino , Nanoestruturas/toxicidade , Tamanho da Partícula , Permeabilidade/efeitos dos fármacos , Inibidores da Agregação Plaquetária/administração & dosagem , Inibidores da Agregação Plaquetária/farmacocinética , Inibidores da Agregação Plaquetária/toxicidade , Coelhos , Pele/efeitos dos fármacos , Absorção Cutânea , Ácidos Esteáricos/química , Ácidos Esteáricos/toxicidade , Triglicerídeos/química , Triglicerídeos/toxicidade
4.
Vet Parasitol ; 169(3-4): 399-403, 2010 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-20304561

RESUMO

The preparation of neem oil microemulsion and its acaricidal activity in vitro was developed in this study. In these systems, the mixture of Tween-80 and the sodium dodecyl benzene sulfonate (SDBS) (4:1, by weight) was used as compound surfactant; the mixture of compound surfactant and hexyl alcohol (4:1, by weight) was used as emulsifier system; the mixture of neem oil, emulsifier system and water (1:3.5:5.5, by weight) was used as neem oil microemulsion. All the mixtures were stired in 800 rpm for 15 min at 40 degrees C. The acaricidal activity was measured by the speed of kill. The whole lethal time value of 10% neem oil microemulsion was 192.50 min against Sarcoptes scabiei var. cuniculi larvae in vitro. The median lethal time value was 81.7463 min with the toxicity regression equations of Y=-6.0269+3.1514X. These results demonstrated that neem oil microemulsion was effective against Sarcoptes scabie var. cuniculi larvae in vitro.


Assuntos
Acaricidas/toxicidade , Azadirachta , Glicerídeos/toxicidade , Preparações de Plantas/toxicidade , Sarcoptes scabiei/efeitos dos fármacos , Terpenos/toxicidade , Animais , Benzenossulfonatos/química , Relação Dose-Resposta a Droga , Emulsões , Glicerídeos/química , Preparações de Plantas/química , Polissorbatos/química , Ácidos Esteáricos/isolamento & purificação , Ácidos Esteáricos/toxicidade , Tensoativos/química , Terpenos/química
5.
Biol Pharm Bull ; 32(3): 500-3, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19252304

RESUMO

To determine whether or not a "bolus injection" of soybean-based fat emulsion (SFE), which contains oleic acid (OA), a potent lung-toxic unsaturated C-18 fatty acid, can induce pulmonary dysfunction, we examined the effect of SFE injection on the partial oxygen pressure of arterial blood (Pao2) and pulmonary vascular permeability. In addition, we compared the effect of an injection of SFE with that of OA, soybean oil (a source of SFE), emulsified OA and C-18 fatty acids. Bolus injection of SFE (0.3-4.8 ml/kg) had little effect on Pao2) and pulmonary vascular permeability. Injection of an equivalent amount of OA, on the other hand, significantly decreased Pao2 and increased pulmonary vascular hyper-permeability. This decrease in Pao2 was attenuated by emulsification. Unemulsified soybean oil also induced a decrease in Pao2, although the effect was weaker than that of OA. Other unsaturated C-18 fatty acids (linoleic and linolenic acid) induced a decrease in Pao2 as potent as OA while stearic acid, a C-18 saturated fatty acid, had little effect. Although we did not observe pulmonary toxicity as a result of "bolus injection" of SFE, the chemical form, for example, emulsification and the degree of saturability of the carbon chain, seems to influence the pulmonary toxicities of lipids and fatty acids. Furthermore, the potent pulmonary toxicity of OA seems to depend not only on pulmonary vascular embolization but also pharmacological and/or inflammation-inducing properties.


Assuntos
Emulsões Gordurosas Intravenosas/toxicidade , Ácidos Graxos/toxicidade , Glycine max , Pulmão/efeitos dos fármacos , Troca Gasosa Pulmonar/efeitos dos fármacos , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/fisiopatologia , Animais , Permeabilidade Capilar/efeitos dos fármacos , Emulsões , Cobaias , Ácido Linoleico/toxicidade , Pulmão/irrigação sanguínea , Pulmão/fisiopatologia , Ácido Oleico/toxicidade , Oxigênio/sangue , Pressão Parcial , Óleo de Soja/toxicidade , Ácidos Esteáricos/toxicidade , Ácido alfa-Linolênico/toxicidade
6.
Int J Pharm ; 337(1-2): 291-8, 2007 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-17300887

RESUMO

The cytotoxicity and physical properties of various submicron O/W emulsions and solid lipid nanoparticles for dermal applications were investigated. Droplet size and zetapotential of submicron emulsions depended on the composition of the cosurfactant blend used. The viability of J774 macrophages, mouse 3T3 fibroblasts and HaCaT keratinocytes was significantly reduced in the presence of stearylamine. Nanoparticles consisting of stearic acid or different kinds of adeps solidus could be manufactured when formulated with lecithin, sodium taurocholate, polysorbate 80 and stearylamine. Survival of macrophages was highly affected by stearic acid and stearylamine. In general a viability of more than 90% was observed when semi-synthetic glycerides or hard fat was employed to formulate nanoparticles.


Assuntos
Portadores de Fármacos , Emulsões , Lipídeos/toxicidade , Nanopartículas , Tensoativos/toxicidade , Administração Cutânea , Aminas/toxicidade , Animais , Células 3T3 BALB , Sobrevivência Celular/efeitos dos fármacos , Gorduras/toxicidade , Fibroblastos/efeitos dos fármacos , Humanos , Queratinócitos/efeitos dos fármacos , Lipídeos/administração & dosagem , Lipídeos/química , Macrófagos/efeitos dos fármacos , Camundongos , Tamanho da Partícula , Fosfatidilcolinas/toxicidade , Fosfatidilserinas/toxicidade , Polissorbatos/toxicidade , Óleo de Soja/toxicidade , Ácidos Esteáricos/toxicidade , Tensoativos/administração & dosagem , Tensoativos/química , Ácido Taurocólico/toxicidade , Água/química
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