An epigenomic shift in amygdala marks the transition to maternal behaviors in alloparenting virgin female mice.

Adults of many species will care for young offspring that are not their own, a phenomenon called alloparenting. However, in many cases, nonparental adults must be sensitized by repeated or extended exposures to newborns before they will robustly display parental-like behaviors. To capture neurogenomic events underlying the transition to active parental caring behaviors, we analyzed brain gene expression and chromatin profiles of virgin female mice co-housed with pregnant dams during pregnancy and after birth. After an initial display of antagonistic behaviors and a surge of defense-related gene expression, we observed a dramatic shift in the chromatin landscape specifically in amygdala of the pup-exposed virgin females compared to females co-housed with mother before birth, accompanied by a dampening of anxiety-related gene expression. This epigenetic shift coincided with hypothalamic expression of the oxytocin gene and the emergence of behaviors and gene expression patterns classically associated with maternal care. The results outline a neurogenomic program associated with dramatic behavioral changes and suggest molecular networks relevant to human postpartum mental health.

Three hour abstinence as a treatment for high sperm DNA fragmentation: a prospective cohort study.

PURPOSE: This study sought to compare sperm DNA fragmentation (SDF) in semen specimens after 3 days and then after 3 h of abstinence in men presenting for initial infertility evaluation. METHODS: A prospective cohort study of 112 men undergoing their first semen analysis as part of an infertility work-up was conducted. All participants presented with 3 days of abstinence for a semen analysis and DNA-fragmentation test. Both tests were repeated on a second sample collected 3 h after the first ejaculation. DNA-fragmentation was evaluated with the halo test by one of two technicians blinded to duration of abstinence. Variables analyzed include ejaculate volume, sperm concentration and motility, smoking status, cannabis use, initial specimen DNA fragmentation, and use of sperm-directed anti-oxidant formulations. RESULTS: Among all subjects, DNA fragmentation improved in the 3-h abstinence specimen (34.6 ± 19.4% vs. 23.7 ± 16.0%, p = 0.0001). Among subjects with high DNA fragmentation (> 35%) on the initial specimen, 55% improved into the normal range. Semen volume and sperm concentration decreased (3.1 ± 3.3 ml vs. 1.9 ± 0.8 ml, p < 0.01 and 41 ± 39 vs. 32 ± 31 (millions/ml), p = 0.01), while progressive motility tended to increase. Fifty-eight subjects demonstrated ≥ 30% improvement in SDF in the second specimen as compared to the first. Factors found to correlate with > 30% improvement in DNA fragmentation in the 3-h abstinence specimen compared to 3 days were younger age and use of anti-oxidants. CONCLUSION: High SDF can often be managed with a second ejaculation 3 h after the first in infertile couples, including in males with abnormal semen analyses per the 2010 WHO guide. Apart from SDF levels, changes in sperm quality were not clinically significant in the second specimen and did not increase rates of ICSI. However, a second ejaculation after 3 h probably may reduce the necessity of costly and/or invasive ART strategies.

African indigenous contraception: A review.

In Africa, indigenous methods of contraception continue to play a significant role in preventing unwanted pregnancies despite the introduction and popularity of modern contraceptives. The current review identified the common techniques and practices of African indigenous contraception, and examined their mechanisms and reasons for use. We searched data bases such as Google Scholar, Scopus, Web of Science, EBSCohost, African Journals, Science Direct, textbooks, thesis and dissertations for research articles on African indigenous contraception. The six common techniques of African indigenous contraception included periodic abstinence, withdrawal, breastfeeding, use of herbs, postpartum abstinence and waist bands, whilst practices relate to child (birth) spacing, postponement of first birth (virginity), stopping of reproduction and indigenous emergency contraception. Mother and infant health was stated as one of the reasons for using African indigenous contraception. African indigenous contraception continues to play a critical reproductive role in preventing unwanted pregnancies. However, there is lack of clarity regarding mechanisms, the safety, and efficacy of some techniques.

Religiosity and sexual abstinence among Nigerian youths: does parent religion matter?

BACKGROUND: Religion plays an important role in youth behaviours, making it a significant factor in the discourse on youth sexuality in sub-Saharan Africa. Several studies have found that religion and religiosity play an important role in the sexual behaviours of young people. However, little research in Nigeria has examined the mechanisms through which religiosity influences youth sexual behaviour and if parents' religion moderates this relationship. Guided by the social control theory, this paper contributes to the existing literature by examining the relationship between religiosity and youth sexual behaviour. METHODS: Data for the study came from 2399 male and female youth aged 16-24 years in four states purposively selected from four regions in Nigeria. Abstinence was the sexual behaviour of interest. Logistic regression was used to examine this relationship. RESULTS: Results showed that 68% of the youth had never had sex. Religiosity was a protective factor for youth sexual behaviour and this positive association was still evident even after controlling for other covariates. Youth who were highly religious (OR - 1.81, CI- 1.13-2.88) had significantly higher odds of abstaining compared to their counterparts who were not religious. CONCLUSION: Religiosity is a protective factor for sexual abstinence among youth in Nigeria. Policy makers can work around using religious institutions for behavioural change among youth in Nigeria.

The role of traditional contraceptive methods in family planning among women attending primary health care centers in Kano.

Background: Traditional contraceptive methods (TCMs) have been used by our ancestors for a long time in child spacing before the advent of the modern contraceptive methods but even with the introduction of the modern methods some women prefer and are still using TCMs. Aim: The aim of this study was to assess the utilization of traditional contraceptives in child spacing and its association with family size among women of child-bearing age attending primary healthcare centers in Kano. Materials and Methods: This was a cross-sectional study among 400 women attending primary healthcare centers in Kano. Their sociodemographic characteristics, number of children, knowledge, and use of traditional contraceptives were recorded on a pretested questionnaire. Results: The mean age ± standard deviation (SD) was 29.1 ± 6.22 years. The mean number of children (±SD) was 3.9 ± 2.27. A total number of 280 (70.0%) participants knew about TCMs, but only 147 (36.8%) used these methods and among those that used TCMs, herbal medicine was the most used method (n = 67, 45.6%). There was no statistically significant difference between the mean number of children of the respondents who used traditional contraceptives and those who did not (t = 0.382, df = 398, P = 0.703, 95% confidence interval:-0.374-0.555). Educational status was significantly associated with the use of traditional contraceptives (χ2 = 8.327, P = 0.005). Conclusion: There was more knowledge of traditional than modern contraceptive methods. Herbal medicine was the most commonly used method. There was poor utilization of the modern contraceptive methods and fair utilization of the TCMs. The study showed no clear benefit of traditional contraceptive usage over its nonuse in reducing family size.

RésuméContexte: Les méthodes contraceptives traditionnelles (MTC) ont longtemps été utilisées par nos ancêtres dans l'espacement des naissances avant l'avènement des méthodes contraceptives modernes, mais même avec l'introduction des méthodes modernes certaines femmes préfèrent et utilisent encore les MTC. But: Le but de cette étude est d'évaluer l'utilisation des contraceptifs traditionnels dans l'espacement des naissances et son association avec la taille de la famille parmi les femmes en âge de procréer fréquentant les centres de soins de santé primaires à Kano. Matériels et méthodes: Il s'agissait d'une étude transversale parmi 400 femmes fréquentant les centres de soins de santé primaires à Kano. Leurs caractéristiques sociodémographiques, le nombre d'enfants, la connaissance et l'utilisation de les contraceptifs traditionnels ont été enregistrés sur un questionnaire pré-testé. Résultats: L'âge moyen ± écart type (ET) était de 29,1 ± 6,22 ans. Le nombre moyen d'enfants (± écart-type) était de 3,9 ± 2,27. Un chiffre de 280 (70,0%) connaissait les MTC, mais seulement 147 (36,8%) utilisaient ces méthodes et parmi celles qui utilisaient des MTC, la phytothérapie était la méthode la plus utilisée (n = 67, 45,6%). Il n'y avait pas de différence statistiquement significative entre le nombre moyen d'enfants des répondants qui ont utilisé des contraceptifs traditionnels et ceux qui ne l'ont pas fait (t = 0,382, df = 398, P = 0,703, Intervalle de confiance de 95%: -0,374-0,555). Le statut éducatif était significativement associé à l'utilisation des contraceptifs traditionnels (χ2 = 8,327, P = 0,005). Conclusion: Il y avait plus de connaissance des méthodes contraceptives traditionnelles que modernes. La phytothérapie était la plus méthode couramment utilisée. Les méthodes modernes de contraception et l'utilisation équitable des MTC ont été mal utilisées. L'étude a montré pas de bénéfice clair de l'utilisation traditionnelle de la contraception par rapport à sa non-utilisation pour réduire la taille de la famille. Mots-clés: Planification familiale, rôle, méthodes contraceptives traditionnelles.

Celibate Gay Christians: Sexual Identity and Religious Beliefs and Practices.

The purpose of the study is to explore the experiences of gay Christians who choose celibacy. Chief milestone events, beliefs and attitudes toward God and faith, as well as participants' experiences of church climate toward gay Christians were assessed through a structured interview. Eight interviews were analyzed, using grounded theory methodology and consensual qualitative analysis to identify several salient themes.

How Religious Beliefs and Practices Influence the Psychological Health of Catholic Priests.

Roman Catholic diocesan priests are a subgroup of men with unique religious and spiritual roles, beliefs, and practices. This qualitative study of 15 priests from the mid-Atlantic area of the United States focused on how priests' relationship with God and promises of celibacy and obedience influenced their psychological health. Using a consensual qualitative research (CQR) design, the analysis revealed that participants described their relationship with God as central to their health and contributing to positive outcomes (e.g., sense of connection and support). The influence of their promises of celibacy and obedience were linked to both positive outcomes (e.g., decreased stress, improved relationships) and negative outcomes (e.g., internal conflict, depression/loneliness). This study highlighted the central role that priests' relationship with God has on positive psychological health. Future research is necessary to understand how to maximize the positive effects and minimize the negative effects of priests' promises of celibacy and obedience, which would benefit programs aimed at supporting priests' psychological health.

"By expresse experiment": the doubting midwife Salome in late medieval England.

This article examines late medieval English representations of the startling and apocryphal story of Salome, the skeptical midwife who dares to touch, or at least attempt to touch, the Virgin Mary "in sexu secreto" during a postpartum examination at the nativity. Salome's story originated in the second century, but its late medieval iterations are inflected by a culture interested in evaluating and examining sensory evidence, in both medicine and religion. The story appears in sermon collections, devotional texts, the cycle nativity plays, and John Lydgate's Life of Our Lady, and these variations demonstrate the intersection of gender and experience-based knowledge in medical and devotional contexts. Salome's story provides a unique opportunity to study late medieval interpretations of female medicine, materialism, and spirituality.

Promoting women's human rights: A qualitative analysis of midwives' perceptions about virginity control and hymen 'reconstruction'.

OBJECTIVES: To explore midwives' perceptions regarding virginity control and hymen 'reconstructions', and how these practices can be debated from a gender perspective. METHODS: An international group of 266 midwives answered an open-ended question in a Web survey. The great majority came from the Western world, among them, the majority were from Europe. Data were analysed using qualitative content analysis. RESULTS: Three themes emerged: misogynistic practices that cement the gender order, which revealed how the respondents viewed virginity control and hymen 'reconstructions'; raising public awareness and combatting practices that demean women, which were suggested as strategies by which to combat these practices; and promoting agency in women and providing culturally sensitive care, which were considered to improve health care encounters. CONCLUSIONS: Virginity control and hymen 'reconstructions' are elements of patriarchy, whereby violence and control are employed to subordinate women. To counter these practices, macro and micro-level activities are needed to expand women's human rights in the private and the public spheres. Political activism, international debates, collaboration between sectors such as health care and law-makers may lead to increased gender equality. A women-centred approach whereby women are empowered with agency will make women more capable of combatting virginity control and hymen 'reconstruction'.

Associations between sexual abstinence ideals, religiosity, and alcohol abstinence: a longitudinal study of Finnish twins.

We analyzed prevalence and stability of attitudes endorsing sexual abstinence ideals from late adolescence into early adulthood and studied associations of these attitudes with religiosity and alcohol abstinence in a sexually liberal Nordic society. Our population-based sample of Finnish twins permitted comparisons of co-twins concordant for religiosity but discordant for drinking to evaluate the association of sexual abstinence ideals with alcohol abstinence, controlling for household environment. From age 17 to 24, endorsement of sexual abstinence as a romantic ideal declined from 25% to 15%. Religiosity and alcohol abstinence correlated, both separately and together, with endorsing sexual abstinence. Abstinence ideals were associated with literal belief in fundamental tenets of the Bible. The association of sexual abstinence ideals with alcohol abstinence was confirmed in within-family comparisons of co-twins discordant for drinking but concordant for religiosity. Alcohol-abstinent twins were significantly more likely than their non-alcohol-abstinent twin siblings to endorse sexual abstinence ideals; that result suggests the association of sexual abstinence ideals with abstaining from alcohol is not explained by unmeasured confounds in familial background and structure. Our longitudinal results and analyses of discordant twins suggest that attitudes toward sexual abstinence ideals are embedded within other conservative attitudes and behaviors.

The combination matters--distinct impact of lifestyle factors on sperm quality: a study on semen analysis of 1683 patients according to MSOME criteria.

BACKGROUND: Poor sperm quality can negatively affect embryonic development and IVF outcome. This study is aimed at investigating the influence of various lifestyle factors on semen quality according to MSOME (motile sperm organelle morphology examination) criteria. METHODS: 1683 male patients undergoing assisted reproductive technologies (ART) in our clinic were surveyed about their age, BMI (body mass index), ejaculation frequency, nutrition, sports, sleeping habits and social behavior. Semen samples were collected and evaluation of semen parameters according to MSOME and WHO criteria was performed. Results were grouped and statistically analyzed. RESULTS: Although single parameters had minor effects on sperm parameter, the combination of age, BMI, coffee intake, ejaculatory frequency and duration of sexual abstinence were identified as factors having a negative effect on sperm motility. Additionally, we could demonstrate that MSOME quality was reduced. The negative impact of age, BMI and coffee intake on sperm quality could be compensated if patients had a high ejaculation frequency and shorter periods of sexual abstinence. CONCLUSIONS: Combinations of adverse lifestyle factors could have a detrimental impact on sperm, not only in terms of motility and sperm count but also in terms of sperm head vacuolization. This negative impact was shown to be compensated by higher ejaculation frequency and a shorter period of sexual abstinence. The compensation is most likely due to a shorter storage time in the male gonads, thus reducing the duration of sperms' exposure to reactive oxygen species (ROS).

Which women breastfeed for 2 years or more?

OBJECTIVE: To identify factors associated with continuation of breastfeeding for 2 years or more. METHODS: This was a cohort study that followed 151 children recruited at the Hospital de Clínicas in Porto Alegre, Brazil, from birth until ages ranging from 3 to 5 years. Mothers were interviewed in person in the maternity unit, at 7 and 30 days after delivery, and when their children were from 3 to 5 years old. Interviews were also conducted at 60, 120 and 180 days, by telephone when possible, or during a home visit otherwise. Associations between the outcome (breastfeeding for 2 years or more) and explanatory variables were investigated using Poisson regression within a hierarchical model. RESULTS: The following variables had positive associations with the outcome: mother staying at home with her child for the first 6 months [relative risk (RR) = 2.13; 95% confidence interval (95%CI) 1.12-4.05]; not using a pacifier (RR = 2.45; 95%CI 1.58-3.81); and later introduction of water and/or teas and of other milks. Each extra day that these liquids were not introduced was associated with 0.5% and 0.1% greater probability of the child being breastfed beyond 2 years, respectively. Cohabitation with the child's father had a negative association with the outcome (RR = 0.61; 95%CI 0.37-0.99). CONCLUSION: Mothers staying at home with their children for the first 6 months of their lives, not cohabiting with a partner, not giving their children pacifiers and delaying introduction of water and/or teas and of other milks are characteristics and behaviors associated with continuation of breastfeeding for 2 years or more.

Quem são as mulheres que amamentam por 2 anos ou mais? / Which women breastfeed for 2 years or more?

OBJETIVO: Identificar fatores associados à manutenção do aleitamento materno por 2 anos ou mais. MÉTODOS: Estudo de coorte que acompanhou 151 crianças selecionadas no Hospital de Clínicas de Porto Alegre, do nascimento até a idade de 3 a 5 anos. As mães foram entrevistadas pessoalmente na maternidade, aos 7 e 30 dias após o parto, e quando as crianças tinham entre 3 e 5 anos. As entrevistas aos 60, 120 e 180 dias de vida da criança foram feitas por contato telefônico, sempre que possível. Para testar as associações entre o desfecho (aleitamento materno por 2 anos ou mais) e as variáveis explicativas, utilizou-se regressão de Poisson seguindo modelo hierárquico. RESULTADOS: Mostraram-se associados de forma positiva, com o desfecho: permanência da mãe em casa com a criança nos primeiros 6 meses de vida [risco relativo (RR) = 2,13; intervalo de confiança de 95 por cento (IC95 por cento) 1,12-4,05]; não uso de chupeta (RR = 2,45; IC95 por cento 1,58-3,81); e introdução mais tardia de água e/ou chás e de outros leites na alimentação da criança. Para cada dia a mais sem a introdução desses líquidos, aumentava a probabilidade de a criança ser amamentada por 2 anos ou mais em 0,5 por cento e 0,1 por cento, respectivamente. Coabitação com o pai da criança mostrou associação negativa com o desfecho (RR = 0,61; IC95 por cento 0,37-0,99). CONCLUSÃO: Mãe permanecer em casa com a criança nos primeiros 6 meses de vida, não coabitar com companheiro, não oferecer chupeta e postergar a introdução de água e/ou chás e outros leites na alimentação das crianças são características e comportamentos associados com a manutenção da amamentação por 2 anos ou mais.

OBJECTIVE: To identify factors associated with continuation of breastfeeding for 2 years or more. METHODS: This was a cohort study that followed 151 children recruited at the Hospital de Clínicas in Porto Alegre, Brazil, from birth until ages ranging from 3 to 5 years. Mothers were interviewed in person in the maternity unit, at 7 and 30 days after delivery, and when their children were from 3 to 5 years old. Interviews were also conducted at 60, 120 and 180 days, by telephone when possible, or during a home visit otherwise. Associations between the outcome (breastfeeding for 2 years or more) and explanatory variables were investigated using Poisson regression within a hierarchical model. RESULTS: The following variables had positive associations with the outcome: mother staying at home with her child for the first 6 months [relative risk (RR) = 2.13; 95 percent confidence interval (95 percentCI) 1.12-4.05]; not using a pacifier (RR = 2.45; 95 percentCI 1.58-3.81); and later introduction of water and/or teas and of other milks. Each extra day that these liquids were not introduced was associated with 0.5 percent and 0.1 percent greater probability of the child being breastfed beyond 2 years, respectively. Cohabitation with the child’s father had a negative association with the outcome (RR = 0.61; 95 percentCI 0.37-0.99). CONCLUSION: Mothers staying at home with their children for the first 6 months of their lives, not cohabiting with a partner, not giving their children pacifiers and delaying introduction of water and/or teas and of other milks are characteristics and behaviors associated with continuation of breastfeeding for 2 years or more.

Exposures that may affect sperm DNA integrity: two decades of follow-up in a pregnancy cohort.

Prenatal lifestyle exposures are linked to alterations in conventional semen characteristics. Sperm DNA integrity is another marker of semen quality shown to be altered in mice prenatally exposed to chemicals. From a Danish pregnancy cohort established in 1984-1987, sons were selected for a follow-up study in 2005-2006. We examined associations between prenatal and current lifestyle exposures and DNA fragmentation index (DFI) among 337 men. Sons of overweight mothers had 22% (95% CI: -3; 52) higher DFI than sons of normal weight mothers and sons of parents with a TTP >12 months had 14% (95% CI: -4; 34) higher DFI than sons of parents with a TTP of 0-6 months. Abstinence time was positively associated with DFI (p<0.005). Overweight men had higher DFI compared to normal weight men, however, statistically insignificantly. In conclusion, results indicate that DFI is affected by prenatal exposures, but confidence limits are wide and results statistically insignificant.

ABC for people with HIV: responses to sexual behaviour recommendations among people receiving antiretroviral therapy in Jinja, Uganda.

People living with HIV who are taking antiretroviral therapy (ART) are increasingly involved in 'positive prevention' initiatives. These are generally oriented to promoting abstinence, 'being faithful' (partner reduction) and condom use (ABC). We conducted a longitudinal qualitative study with people living with HIV using ART, who were provided with adherence education and counselling support by a Ugandan non-governmental organisation, The AIDS Support Organisation (TASO). Forty people were selected sequentially as they started ART, stratified by sex, ART delivery mode (clinic- or home-based) and HIV progression stage (early or advanced) and interviewed at enrollment and at 3, 6, 18 and 30 months. At initiation of ART, participants agreed to follow TASO's positive-living recommendations. Initially poor health prevented sexual activity. As health improved, participants prioritised resuming economic production and support for their children. With further improvements, sexual desire resurfaced and people in relationships cemented these via sex. The findings highlight the limitations of HIV prevention based on medical care/personal counselling. As ART leads to health improvements, social norms, economic needs and sexual desires increasingly influence sexual behaviour. Positive prevention interventions need to seek to modify normative and economic influences on sexual behaviour, as well as to provide alternatives to condoms.

The relationship of character strengths to sexual behaviors and related risks among African American adolescents.

BACKGROUND: Relatively few studies have examined positive character traits that are associated with lower HIV/STD risks. PURPOSE: In the present study, the relationships of character strengths with sexual behaviors and attitudes were assessed among 383 African-American adolescents. METHOD: Character strengths were measured using the Values in Action Inventory of Strengths. Because the strengths were highly inter-correlated, stepwise discriminant function analyses were utilized to assess their independent associations with sexual behaviors and attitudes. RESULTS: Greater Love of Learning was related to self-reported abstinence from sexual intercourse for boys and self-reported abstinence from drug use for boys and girls. Greater Love of Learning and Curiosity were related to the belief in no premarital sex for boys, whereas only Curiosity was significant for girls. Prudence was related to reported abstinence from sexual intimacy (e.g., touching or kissing). Judgment was related to sexual initiation efficacy for girls and boys, whereas Leadership was only significant for girls. CONCLUSIONS: The findings suggest that character strengths may be associated with lower levels of sexual behaviors and sex-related beliefs among a sample of African-American adolescents.