RESUMO
Dioscorea bulbifera L. (DBL), a traditional Chinese medicine, is a well-known herb with hepatotoxicity, and the biochemical mechanisms of the toxic action remain unknown. Diosbulbin B (DSB), a major component of DBL, can induce severer liver injury which requires cytochrome P450-catalyzed oxidation of the furan ring. It is reported that a cis-enedial reactive intermediate resulting from metabolic activation of DSB can react with thiols and amines to form pyrrole or pyrroline derivatives. In this study, we investigated the interaction of the reactive intermediate with polyamines, biogenic amines, and amino acids involved in the polyamine metabolic pathway, including putrescine, spermidine, spermine, histamine, arginine, ornithine, lysine, glutamine, and asparagine. Seven DSB-derived amine adducts were detected in microsomal incubations supplemented with DSB and individual amines. Six adducts were observed in cultured rat primary hepatocytes after exposure to DSB. DSB was found to induce apoptosis and cell death in time- and concentration-dependent manners. Apparently, the observed apoptosis was associated with the detected amine adduction. The findings facilitate the understanding of the mechanisms of toxic action of DSB.
Assuntos
Aminoácidos/metabolismo , Aminas Biogênicas/metabolismo , Compostos Heterocíclicos de 4 ou mais Anéis/farmacologia , Poliaminas/metabolismo , Ativação Metabólica , Animais , Apoptose/efeitos dos fármacos , Células Cultivadas , Hepatócitos/metabolismo , Masculino , Ratos Sprague-DawleyRESUMO
A precise and unambiguous quantitative strategy for six biogenic amines in dry fermented mutton sausage with liquid chromatography tandem quadrupole Orbitrap mass spectrometry was developed to investigate the inhibitory effects of star anise, amomum tsao-ko, clove, cassia, fennel, bay leaf, and nutmeg on the accumulation of biogenic amines. Compared the data-dependent MS2 fragmentation (dd-MS2), variable data-independent acquisition (vDIA), and multiplex data-independent acquisition (mDIA), and the mDIA acquisition mode was selected for further analysis. The LODs and LOQs were 0.9-1.5 µg kg-1 and 2.9-5.0 µg kg-1. The maximum inhibition of spice on tryptamine, putrescine, spermidine, 2-phenylethylamine, tyrosamine, and histamine were 21.8%, 19.3%, 27.5%, 24.6%, 18.7% and 24.4%, which revealed that the accumulation of BAs was significantly inhibited by spice extracts, while cassia and fennel extracts showed the best effect. Thus, the established method can provide a better reference for dry fermented mutton sausage quality, safety and trade analysis.
Assuntos
Aminas Biogênicas/análise , Alimentos Fermentados/análise , Produtos da Carne , Especiarias , Aminas Biogênicas/metabolismo , Cassia/química , Cromatografia Líquida de Alta Pressão , Fermentação , Foeniculum , Cromatografia Gasosa-Espectrometria de Massas , Histamina/metabolismo , Produtos da Carne/análise , Extratos Vegetais/análise , Extratos Vegetais/química , Carne Vermelha/análise , Especiarias/análise , Espectrometria de Massas em Tandem , Compostos Orgânicos Voláteis/análiseRESUMO
The effects of onion or caraway on changes in the content of biogenic amines were examined in sauerkraut during a fermentation process at 18⯰C or 31⯰C for 14â¯days and subsequent storage at 4⯰C for 12â¯weeks. The amines were analysed by high-performance liquid chromatography with pre-column benzoylation. Total biogenic-amine concentration at the end of the fermentation was lower at 31⯰C than at 18⯰C. However, at this lower temperature, the presence of caraway or onion more significantly (than at 31⯰C) reduced the total biogenic-amine content as compared to the control sample without an additive. After 12â¯weeks of refrigerated storage, concentrations of phenethylamine, tryptamine, and tyramine in the sauerkraut fermented with caraway (and concentrations of putrescine and tryptamine in the sauerkraut fermented with onion) at 31⯰C increased as compared to the samples on the last day of fermentation, but did not pose a risk for consumer health.
Assuntos
Aminas Biogênicas/metabolismo , Carum , Alimentos Fermentados , Armazenamento de Alimentos/métodos , Cebolas , Aminas Biogênicas/análise , Brassica , Cromatografia Líquida de Alta Pressão , Temperatura Baixa , Fermentação , Alimentos Fermentados/análise , Humanos , Concentração de Íons de Hidrogênio , Putrescina/análise , Putrescina/metabolismo , Paladar , Tiramina/análise , Tiramina/metabolismoRESUMO
Food biogenic amines, in particular, histamine, are often responsible for various enteric and vascular dysfunctions. Several years ago, the oral administration of copper-containing diamine oxidase (DAO), also called histaminase, able to oxidatively deaminate biogenic amines, had been suggested as a food supplement to control food allergy and enteric dysfunctions. This report is aimed to generate a global image on the behavior of orally administrated DAO dosage forms in the intestinal tract. The catalytic stability of DAO from Lathyrus sativus seedlings in various simulated intestinal media with different pH and containing different association of cholic acids, pancreatic proteases, bicarbonate, lipids, or alcohol was investigated. Cholic acids and lipids protected the enzyme in the simulated intestinal fluids. However, they were not able to protect against the inhibitory effect of 24-36% (v/v) ethanol. These observations may be relevant for oral administration of enzymes as food supplements or therapeutic bioactive agents.
Assuntos
Amina Oxidase (contendo Cobre)/química , Ácidos Cólicos/metabolismo , Mucosa Intestinal/metabolismo , Lathyrus/enzimologia , Proteínas de Plantas/química , Amina Oxidase (contendo Cobre)/metabolismo , Aminas Biogênicas/metabolismo , Ácidos Cólicos/química , Estabilidade Enzimática , Humanos , Concentração de Íons de Hidrogênio , Intestinos/química , Lathyrus/química , Lathyrus/metabolismo , Modelos Biológicos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMO
Biogenic amines are direct mediators of interactions between immune and nervous systems implicated in the pathogenesis of multiple sclerosis (MS). Recently, great attention has been drawn to studying the effects of biogenic amines on Th17-cells, which play one of the central roles in the development of inflammatory lesions in MS. Results of these studies suggest that, depending on the activation of particular receptors, biogenic amines can both enhance and inhibit Th17-cell functions. Based on these data, targeting biogenic amines and their receptors could be explored as a new kind of additional disease-modifying treatment of MS.
Assuntos
Aminas Biogênicas/metabolismo , Esclerose Múltipla/imunologia , Células Th17/imunologia , Animais , HumanosRESUMO
Experimental studies have been carried out on rats to understand the influence of immobilization stress (IMS), a psychological stressor and forced swim stress (FSS), a physical stressor in the neurotoxicity of lambda-cyhalothrin (LCT), a new generation type II synthetic pyrethroid with extensive applications. No significant change in plasma corticosterone levels and blood brain barrier (BBB) permeability was observed in rats subjected to IMS (one session of 15min/day), FSS (one session of 3min/day) for 28days or LCT treatment (3.0mg/kg body weight, p.o. suspended in groundnut oil) for 3days (26th, 27th and 28th day) as compared to controls. Marginal changes in the levels of biogenic amines and their metabolites (NE, EPN, DA, HVA, DOPAC, 5-HT) in hypothalamus, frontal cortex, hippocampus, and corpus striatum were observed in rats subjected to IMS or FSS or LCT alone as compared to controls. It was interesting to note that pre-exposure to IMS or FSS followed by LCT treatment for 3days caused a marked increase in plasma corticosterone levels associated with disruption in the BBB permeability as compared to rats exposed to IMS or FSS or LCT alone. Pre-exposure to IMS or FSS followed by LCT treatment for 3days resulted to alter the levels of biogenic amines and their metabolites in hypothalamus, frontal cortex, hippocampus, and corpus striatum as compared to rats exposed to IMS or FSS or LCT alone. Although neurochemical changes were more intense in rats pre-exposed to IMS as compared to those subjected to FSS on LCT treatment, the results indicate that both psychological and physical stress could be important influencing factors in the neurotoxicity of LCT.
Assuntos
Aminas Biogênicas/metabolismo , Barreira Hematoencefálica/efeitos dos fármacos , Encéfalo/efeitos dos fármacos , Inseticidas/toxicidade , Nitrilas/toxicidade , Piretrinas/toxicidade , Estresse Psicológico/metabolismo , Animais , Barreira Hematoencefálica/metabolismo , Encéfalo/metabolismo , Permeabilidade Capilar/efeitos dos fármacos , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Corticosterona/sangue , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Hipotálamo/efeitos dos fármacos , Hipotálamo/metabolismo , Masculino , Ratos Wistar , Restrição Física , Estresse FisiológicoRESUMO
BACKGROUND: Diets containing high levels of carbohydrates provoke a rapid decrease of rumen pH and high levels of biogenic amines and lipopolysaccharides (LPS), which severely impair the health and performance of ruminants. The goal of this study was to evaluate the effects of sodium bicarbonate (BC) buffer on rumen fermentation, levels of LPS and biogenic amine, and composition of rumen microbiota using in vitro rumen cultures. RESULTS: Sodium bicarbonate supplementation increased (P < 0.05) the final pH levels and concentrations of total volatile fatty acids and LPS, as well as the proportions of acetate, propionate, isobutyrate, isovalerate and valerate, and it decreased (P < 0.05) the proportion of butyrate and the levels of lactic acid, methylamine, tryptamine, tyramine, histamine and putrescine compared with the control. Pyrosequencing of the 16S rRNA gene showed that BC inclusion increased (P < 0.05) the bacterial diversity index compared with the control. Adding BC also decreased (P < 0.05) the relative abundance of Streptococcus and Butyrivibrio and increased (P < 0.05) the proportions of Ruminococcus, Succinivibrio and Prevotella. CONCLUSION: Sodium bicarbonate supplementation has beneficial effects in the reduction of bioamine levels and the increase in ruminal pH, and in modifying the microbial ecology of the rumen; however, it results in an accumulation of LPS under high-grain diet conditions. © 2016 Society of Chemical Industry.
Assuntos
Bactérias/efeitos dos fármacos , Aminas Biogênicas/metabolismo , Ácidos Graxos Voláteis/metabolismo , Microbioma Gastrointestinal/efeitos dos fármacos , Lipopolissacarídeos/metabolismo , Rúmen , Bicarbonato de Sódio/farmacologia , Ração Animal , Animais , Bactérias/crescimento & desenvolvimento , Bactérias/metabolismo , Biodiversidade , Soluções Tampão , Bovinos , Dieta , Suplementos Nutricionais , Feminino , Fermentação , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Microbiota , Rúmen/metabolismo , Rúmen/microbiologiaRESUMO
The presence of high levels of biogenic amines is detrimental to the quality and safety of fish sauce. This study investigated the effects of ethanol extracts of spices, including garlic, ginger, cinnamon, and star anise extracts, in reducing the accumulation of biogenic amines during fish sauce fermentation. The concentrations of biogenic amines, which include histamine, putrescine, tyramine, and spermidine, all increased during fish sauce fermentation. When compared with the samples without spices, the garlic and star anise extracts significantly reduced these increases. The greatest inhibitory effect was observed for the garlic ethanolic extracts. When compared with controls, the histamine, putrescine, tyramine, and spermidine contents and the overall biogenic amine levels of the garlic extract-treated samples were reduced by 30.49%, 17.65%, 26.03%, 37.20%, and 27.17%, respectively. The garlic, cinnamon, and star anise extracts showed significant inhibitory effects on aerobic bacteria counts. Furthermore, the garlic and star anise extracts showed antimicrobial activity against amine producers. These findings may be helpful for enhancing the safety of fish sauce.
Assuntos
Bactérias , Aminas Biogênicas/metabolismo , Fermentação , Produtos Pesqueiros/análise , Magnoliopsida , Extratos Vegetais/farmacologia , Especiarias , Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Bactérias/metabolismo , Cinnamomum zeylanicum , Produtos Pesqueiros/microbiologia , Microbiologia de Alimentos , Alho , Zingiber officinale , Histamina/metabolismo , Humanos , Illicium , Putrescina/metabolismo , Espermidina/metabolismo , Tiramina/metabolismoRESUMO
The article presents a detailed analysis of correlations between the content of a variety of biogenic amines in the hypothalamic structures responsible for the luteinizing hormone releasing hormone synthesis and secretion (the medial preoptic area and median eminence) and such independent factors as total L-homocysteine plasma level elevation induced by L-methionine loading and aging. Both a nature and a pattern of changes in oxidative stress profile were evaluated. It was shown that ageing, when compared to hyperhomocysteinemia, is a determining factor influencing biogenic amine content in the studied hypothalamic structures. Unlike antioxidant defense system profile, considerable changes in macromolecule oxidative modification were not found, which evidences a balanced activity of pro- and antioxidant systems in the hypothalamus.
Assuntos
Envelhecimento , Aminas Biogênicas/metabolismo , Hormônio Liberador de Gonadotropina , Homocisteína , Hiper-Homocisteinemia/metabolismo , Hipotálamo , Envelhecimento/sangue , Envelhecimento/metabolismo , Animais , Feminino , Hormônio Liberador de Gonadotropina/biossíntese , Hormônio Liberador de Gonadotropina/metabolismo , Homocisteína/sangue , Homocisteína/metabolismo , Hipotálamo/metabolismo , Hipotálamo/patologia , Estresse Oxidativo/fisiologia , Ratos , Ratos WistarRESUMO
AIMS: D-Galactose (D-gal) induced accelerated senescence has been used to develop an aging model for brain. Previously, long term administration of a wide range of doses has been used for this purpose. In the present study we investigate whether short term administration of a high dose of D-gal in rats induces significant signs and symptoms similar to natural aging. MAIN METHODS: Young rats were injected intraperitoneally with D-gal at a dose of 300 mg/ml/kg for one week. Behavioral analysis for depression and anxiety like symptoms were monitored by forced swim test (FST) and light/dark transition (LDT) test. Assessment of memory was done using the Morris water maze (MWM), passive avoidance test (PAT) and elevated plus maze (EPM) test. Biochemical analysis was done for estimation of antioxidant enzymes and acetylcholinesterase. Determination of brain biogenic amines was performed by HPLC-EC. KEY FINDINGS: Short term administration of D-gal significantly altered behavioral, biochemical and neurochemical responses in rats. D-Gal injected rats exhibited depressogenic and anxiogenic behaviors while memory was also significantly impaired in these rats. Brain lipid peroxidation and superoxide dismutase activity were significantly increased while catalase and glutathione peroxidase decreased. Increased activity of acetylcholinesterase was also exhibited by D-gal injected rats while brain biogenic amines were significantly decreased. Food intake and growth rate were however comparable in both groups. SIGNIFICANCE: Together the behavioral, biochemical and neurochemical impairments following the high dose of D-gal suggest that symptoms similar to natural aging may be developed in rats in as early as one week.
Assuntos
Envelhecimento/fisiologia , Comportamento Animal/fisiologia , Encéfalo/fisiologia , Galactose/farmacologia , Memória/fisiologia , Acetilcolinesterase/metabolismo , Animais , Antioxidantes/metabolismo , Aminas Biogênicas/metabolismo , Catalase/metabolismo , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Galactose/administração & dosagem , Glutationa Peroxidase/metabolismo , Peroxidação de Lipídeos/fisiologia , Masculino , Aprendizagem em Labirinto , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Mikania scandens, a twining herb that grows as a weed in India and Bangladesh is used as vegetables and is a good source of vitamin A, C, B complex, mikanin, sesquiterpenes, betasitosterin, stigmasterol and friedelin. The present communication reports CNS depressant activities with special emphasis to brain biogenic amines in mice. Ethanol extract of leaves of M. scandens (EEMS) was prepared by Soxhalation and analyzed chemically. EEMS potentiated sleeping time induced by pentobarbitone, diazepam and meprobamate and showed significant reduction in the number of writhes and stretches. EEMS caused significant protection against pentylene tetrazole-induced convulsion and increased catecholamines and brain amino acids level significantly. Results showed that EEMS produced good CNS depressant effects in mice.
Assuntos
Aminas Biogênicas/metabolismo , Encéfalo/efeitos dos fármacos , Depressores do Sistema Nervoso Central/farmacologia , Mikania/química , Extratos Vegetais/farmacologia , Analgésicos/isolamento & purificação , Analgésicos/farmacologia , Animais , Anticonvulsivantes/isolamento & purificação , Anticonvulsivantes/farmacologia , Encéfalo/metabolismo , Depressores do Sistema Nervoso Central/isolamento & purificação , Relação Dose-Resposta a Droga , Etanol/química , Feminino , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Reflexo/efeitos dos fármacos , Convulsões/induzido quimicamente , Convulsões/prevenção & controle , Tetrazóis , Testes de Toxicidade AgudaRESUMO
This paper evaluates the nutritional values and stability during refrigerated storage of fresh beef merguez sausage as affected by a reformulation process which modified the fat content both by reducing fat (replacing beef fat with konjac gel) and incorporating olive oil (replacing beef fat with olive oil stabilized in a konjac matrix) and by reducing sodium content, replacing sodium chloride with a salt mixture (containing potassium chloride, calcium chloride and magnesium chloride). A preservative (sodium metabisulphite) was also used to extend the shelf-life of the product. The fat was reduced by 32 to 80% and sodium by over 36%. The reformulation did not negatively affect the sensory evaluation. Low microbiota growth rate and biogenic amines were attributed mainly to the presence of sodium metabisulphite. This preservative could be used in the reformulation to enhance safety and/or extend the shelf-life of this type of product.
Assuntos
Amorphophallus , Gorduras na Dieta , Conservação de Alimentos/métodos , Produtos da Carne/análise , Óleos de Plantas , Cloreto de Sódio na Dieta , Sódio , Animais , Aminas Biogênicas/metabolismo , Bovinos , Cloretos , Comportamento do Consumidor , Dieta com Restrição de Gorduras , Dieta Hipossódica , Microbiologia de Alimentos , Conservantes de Alimentos , Armazenamento de Alimentos/métodos , Humanos , Produtos da Carne/microbiologia , Produtos da Carne/normas , Azeite de Oliva , Preparações de Plantas , Refrigeração , Sais , SulfitosRESUMO
ADAR2 transgenic mice misexpressing the RNA editing enzyme ADAR2 (Adenosine Deaminase that act on RNA) show characteristics of overeating and experience adult onset obesity. Behavioral patterns and brain changes related to a possible addictive overeating in these transgenic mice were explored as transgenic mice display chronic hyperphagia. ADAR2 transgenic mice were assessed in their food preference and motivation to overeat in a competing reward environment with ad lib access to a running wheel and food. Metabolic activity of brain and peripheral tissue were assessed with [(18) F] fluorodeoxyglucose positron emission tomography (FDG-PET) and RNA expression of feeding related genes, ADAR2, dopamine and opiate receptors from the hypothalamus and striatum were examined. The results indicate that ADAR2 transgenic mice exhibit, (1) a food preference for diets with higher fat content, (2) significantly increased food intake that is non-distractible in a competing reward environment, (3) significantly increased messenger RNA (mRNA) expressions of ADAR2, serotonin 2C receptor (5HT2C R), D1, D2 and mu opioid receptors and no change in corticotropin-releasing hormone mRNAs and significantly reduced ADAR2 protein expression in the hypothalamus, (4) significantly increased D1 receptor and altered bioamines with no change in ADAR2, mu opioid and D2 receptor mRNA expression in the striatum and (5) significantly greater glucose metabolism in the hypothalamus, brain stem, right hippocampus, left and right mid brain regions and suprascapular peripheral tissue than controls. These results suggest that highly motivated and goal-oriented overeating behaviors of ADAR2 transgenic mice are associated with altered feeding, reward-related mRNAs and hyperactive brain mesolimbic region.
Assuntos
Adenosina Desaminase/genética , Hiperfagia/fisiopatologia , Hipotálamo/fisiopatologia , Adenosina Desaminase/metabolismo , Animais , Aminas Biogênicas/metabolismo , Hormônio Liberador da Corticotropina/genética , Hormônio Liberador da Corticotropina/metabolismo , Dieta Hiperlipídica , Ingestão de Alimentos , Comportamento Alimentar , Fluordesoxiglucose F18 , Glucose/metabolismo , Objetivos , Hiperfagia/genética , Hiperfagia/metabolismo , Hipotálamo/metabolismo , Camundongos , Camundongos Transgênicos , Tomografia por Emissão de Pósitrons , Proteínas de Ligação a RNA , Receptor 5-HT2C de Serotonina/genética , Receptor 5-HT2C de Serotonina/metabolismo , Receptores Dopaminérgicos/genética , Receptores Dopaminérgicos/metabolismo , Receptores Opioides mu/genética , Receptores Opioides mu/metabolismo , Recompensa , Transcrição GênicaRESUMO
Low-fat pork liver pâtés enriched with n-3 PUFA/konjac gel were formulated by replacing (totally or partially) pork backfat by a combination of healthier oils (olive, linseed and fish oils) and konjac gel. Lipid oxidation, microbiological changes and biogenic amine (BA) formation were studied in healthier-lipid pâtés during chill storage (85 days, 2 °C). Increasing unsaturated fatty acid levels favoured lipid oxidation, although the levels reached were low throughout the storage period, ranging from 0.113 to 0.343 mg malonaldehyde/kg sample. Neither the formulation nor the time in storage affected the microbial load. Biogenic amine contents of products (the sum of initial concentrations and amines formed during storage) varied according to the type of BA but were far below levels that could constitute a consumer health hazard.
Assuntos
Amorphophallus/química , Aminas Biogênicas/análise , Substitutos da Gordura/química , Ácidos Graxos Ômega-3/análise , Peroxidação de Lipídeos , Fígado/química , Produtos da Carne/análise , Animais , Aminas Biogênicas/metabolismo , Dieta/etnologia , Dieta com Restrição de Gorduras , Substitutos da Gordura/efeitos adversos , Óleos de Peixe/química , Qualidade dos Alimentos , Armazenamento de Alimentos , Géis , Óleo de Semente do Linho/química , Fígado/metabolismo , Produtos da Carne/microbiologia , Azeite de Oliva , Óleos de Plantas/química , Tubérculos/química , Refrigeração , Espanha , Sus scrofaRESUMO
Water-soluble nanoparticles of curcumin were synthesized, characterized and applied as a stable detoxifying agent for arsenic poisoning. Chitosan nanoparticles of less than 50 nm in diameter containing curcumin were prepared. The particles were characterized by TEM, DLS and FT-IR. The therapeutic efficacy of the encapsulated curcumin nanoparticles (ECNPs) against arsenic-induced toxicity in rats was investigated. Sodium arsenite (2mg/kg) and ECNPs (1.5 or 15 mg/kg) were orally administered to male Wistar rats for 4 weeks to evaluate the therapeutic potential of ECNPs in blood and soft tissues. Arsenic significantly decreased blood δ-aminolevulinic acid dehydratase (δ-ALAD) activity, reduced glutathione (GSH) and increased blood reactive oxygen species (ROS). These changes were accompanied by increases in hepatic total ROS, oxidized glutathione, and thiobarbituric acid-reactive substance levels. By contrast, hepatic GSH, superoxide dismutase and catalase activities significantly decreased on arsenic exposure, indicative of oxidative stress. Brain biogenic amines (dopamine, norepinephrine and 5-hydroxytryptamine) levels also showed significant changes on arsenic exposure. Co-administration of ECNPs provided pronounced beneficial effects on the adverse changes in oxidative stress parameters induced by arsenic. The results indicate that ECNPs have better antioxidant and chelating potential (even at the lower dose of 1.5 mg/kg) compared to free curcumin at 15 mg/kg. The significant neurochemical and immunohistochemical protection afforded by ECNPs indicates their neuroprotective efficacy. The formulation provides a novel therapeutic regime for preventing arsenic toxicity.
Assuntos
Intoxicação por Arsênico/tratamento farmacológico , Arsênio/toxicidade , Curcumina/administração & dosagem , Nanopartículas/administração & dosagem , Nanopartículas/química , Animais , Arsênio/farmacocinética , Intoxicação por Arsênico/sangue , Aminas Biogênicas/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Curcumina/farmacologia , Glutationa/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Distribuição TecidualRESUMO
BACKGROUND: Curcumin is the active principle of Curcuma longa, one of the widely used components in the traditional system of medicine in India. Despite its efficacy in experimental studies aiming at neuronal disorders like depression, curcu-min's poor water solubility challenges the production of therapeutic formulations. This study investigates the antidepressant-like activity of novel water-soluble curcumin formulations, dispensed in three different concentrations. Further, the study comparatively evaluates St. John's wort (SJW), another herbal preparation. METHODS: These compounds were evaluated in the forced swimming test in mice, and the corresponding changes in the neurotransmitter levels were measured. RESULTS: Three water-soluble curcumin formulations, C-5, C-20 and C-50 (50-200 mg/kg p.o.) decreased the immobility period, and increased serotonin and dopamine levels in the brain tissues. A subeffective dose (50 mg/kg) of these formulations enhanced the antidepressant-like effect of classical antidepressants with varied mechanisms of action. In addition, an SJW dose of 25 mg/kg showed a significant antidepressant-like effect in all the behavioral studies and also significantly increased brain neurotransmitter levels, especially that of serotonin. The effects produced by C-5 were comparable with those of SJW and fluoxetine, respectively. CONCLUSION: In all these observations, the water-soluble formulations showed a significant antidepressant-like effect, including enhancement of neurotransmitter levels as compared to the similar dose of a conventional curcumin preparation. Thus, these formulations may be used as a novel treatment option in the management of mental depression.
Assuntos
Antidepressivos/uso terapêutico , Química Farmacêutica/métodos , Curcumina/uso terapêutico , Depressão/tratamento farmacológico , Hypericum/química , Fitoterapia/psicologia , Extratos Vegetais/uso terapêutico , Animais , Antidepressivos/química , Antidepressivos/farmacologia , Aminas Biogênicas/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Curcumina/química , Curcumina/farmacologia , Depressão/metabolismo , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos/métodos , Avaliação Pré-Clínica de Medicamentos/psicologia , Quimioterapia Combinada/psicologia , Resposta de Imobilidade Tônica/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos , Atividade Motora/efeitos dos fármacos , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Solubilidade , Fatores de TempoRESUMO
AIMS: Lactobacillus brevis IOEB 9809 is able to produce both tyramine and putrescine via tyrosine decarboxylase and agmatine deiminase enzymes, respectively, when cultured on synthetic media. The aims of this study were to assess the expression of L. brevis IOEB 9809 tdc and aguA1 genes, during wine fermentation and to evaluate the effect of substrate availability and pH on tdc and aguA1 expression, as well as on biogenic amine production and L. brevis viability. METHODS AND RESULTS: The relative expression of L. brevis IOEB 9809 tdc and aguA1 genes was analysed in wine by quantitative real-time RT-PCR (qRT-PCR) during a period of incubation of 30 days. Cell viability, pH values, putrescine and tyramine concentration were monitored throughout the experiments. CONCLUSIONS: The wine trials indicated that L. brevis IOEB 9809 is able to produce both tyramine and putrescine during wine fermentation. Increased cell viability was also observed in wine supplemented with tyrosine or agmatine. qRT-PCR analysis suggests a strong influence of substrate availability on the expression of genes coding for tyrosine decarboxylase and agmatine deiminase in L. brevis IOEB 9809. Less evident is the relationship between putrescine and tyramine production and tolerance to wine pH. SIGNIFICANCE AND IMPACT OF STUDY: To our knowledge, this study represents the first assessment of relative expression of L. brevis IOEB 9809 genes involved in biogenic amine production in wine. Furthermore, an effect of biogenic amine production on viability of L. brevis during wine fermentation was established.
Assuntos
Hidrolases/metabolismo , Levilactobacillus brevis/enzimologia , Tirosina Descarboxilase/metabolismo , Vinho/microbiologia , Agmatina/metabolismo , Aminas Biogênicas/análise , Aminas Biogênicas/metabolismo , Fermentação , Humanos , Hidrolases/genética , Levilactobacillus brevis/genética , Putrescina/biossíntese , Putrescina/metabolismo , Tiramina/biossíntese , Tiramina/metabolismo , Tirosina/metabolismo , Tirosina Descarboxilase/genéticaRESUMO
The present study was taken up to see the effect of chicoric acid (CA) on behavioral and biochemical alterations induced by chronic restraint stress in experimental Swiss albino mice. CA at 1mg/kg dose level exhibited considerable antidepressant activity as shown by significant decrease in immobility period in the Porsolt's swim stress-induced behavioral despair test and escape failures in Learned "helplessness test". The antidepressant activity shown by CA can be attributed to its modulating effect on nor-adrenaline (NA), dopamine (DA) and 5- hydroxy tryptamine (5-HT) as shown by their quantification in CA treated chronically stressed mice. Further, a significant antioxidant effect was exhibited by CA as shown by estimation of lipid peroxidation, glutathione (GSH) and glycogen in liver of chronically stressed mice. It also normalized altered values of serum glucose, triglycerides, aspartate aminotransferase (AST) alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in a dose dependent manner. The stress busting potential of CA was further confirmed by its regulating effect on raised plasma corticosterone levels and significant attenuation of the depleted ascorbic acid, cholesterol and corticosterone levels in adrenal glands. Thus, our results suggest that CA possesses considerable stress busting potential, and that anti-oxidation may be one of the mechanisms underlying its antistress action.
Assuntos
Aprendizagem da Esquiva/efeitos dos fármacos , Ácidos Cafeicos/farmacologia , Desamparo Aprendido , Estresse Psicológico/metabolismo , Succinatos/farmacologia , Animais , Aprendizagem da Esquiva/fisiologia , Aminas Biogênicas/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Ácidos Cafeicos/isolamento & purificação , Ácidos Cafeicos/uso terapêutico , Doença Crônica , Relação Dose-Resposta a Droga , Masculino , Camundongos , Componentes Aéreos da Planta , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/psicologia , Succinatos/isolamento & purificação , Succinatos/uso terapêuticoRESUMO
Oil (healthier lipid combination of olive, linseed and fish oils)-in-water emulsions stabilized with different protein systems (prepared with sodium caseinate (SC), soy protein isolate (SPI), and microbial transglutaminase (MTG)) were used as pork backfat replacers in low-fat frankfurters. Microstructure, lipid oxidation, nitrite content, microbiological changes and biogenic amine formation of frankfurters were analyzed and found to be affected by the type of oil-in-water emulsion and by chilling storage (2° C, 41 days). Although the lipid oxidation levels attained were low, replacement of animal fat by healthier oil combinations in frankfurter formulation did promote a slight increase in lipid oxidation. Residual nitrite was affected (P < 0.05) by formulation and storage. Only 51-61% of the added nitrite was detectable in the product after processing and 17-46% at the end of storage. The microbial population was low in all formulations during chilling storage. Spermine was the most abundant amine (19-20 mg/kg), but similar in level to all samples.
Assuntos
Aminas Biogênicas/metabolismo , Manipulação de Alimentos/métodos , Metabolismo dos Lipídeos , Produtos da Carne/análise , Nitritos/análise , Animais , Caseínas/química , Fenômenos Químicos , Cromatografia por Troca Iônica , Emulsões/química , Óleos de Peixe/química , Óleo de Semente do Linho/química , Produtos da Carne/microbiologia , Azeite de Oliva , Oxirredução , Óleos de Plantas/química , Proteínas de Soja/química , Suínos , Transglutaminases/químicaRESUMO
This study was undertaken to examine whether cadmium oral exposure modifies biogenic amine concentration at hypothalamic level in adult male rats, and to investigate the possible modulatory effects of melatonin against cadmium-induced changes on these neurotransmitters. For this purpose, rats were exposed to cadmium (25 mg/l of CdCl(2) in the drinking water) with or without melatonin (30 µg/rat/day intraperitoneally) for 30 days. Norepinephrine (NE), dopamine (DA), serotonin (5-HT), 3,4-dihydroxyphenyl acetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA) were quantified by high performance liquid chromatography (HPLC). Oral cadmium administration led to decrease of NE, DA, and 5-HT content and DA turnover within the three hypothalamic regions examined, and therefore an inhibition of 5-HT turnover at posterior hypothalamus. Sensitivity to melatonin was specific to the hypothalamic region evaluated. Thus, the anterior hypothalamus was not nearly sensitive to exogenously administered melatonin, whereas the neurohormone decreased the content of these amines in the mediobasal hypothalamus, and melatonin increased it in the posterior hypothalamic region. Melatonin effectively prevented some cadmium-induced alterations on hypothalamic amine concentration. This is the case of DA in the anterior and posterior hypothalamus, and 5-HT metabolism in the posterior hypothalamic region. In conclusion, the obtained results indicate that melatonin treatment may be effective modulating some neurotoxic effects induced by cadmium exposure, and, more to the point, a possible role of this indolamine as a preventive agent for environmental or occupational cadmium contamination.