Assuntos
Anticorpos , Linfócitos B , Terapia Biológica , Engenharia Celular , Engenharia de Proteínas , Anticorpos/genética , Anticorpos/imunologia , Anticorpos/metabolismo , Linfócitos B/imunologia , Linfócitos B/metabolismo , COVID-19/terapia , Controle de Doenças Transmissíveis , Doenças Transmissíveis/imunologia , Humanos , Imunização Passiva , Soroterapia para COVID-19RESUMO
Programmed cell death ligand 1 (PD-L1) blockade has achieved great success in cancer immunotherapy; however, the response of triple-negative breast cancer (TNBC) to PD-L1 antibodies is limited. To address this challenge, we use the bromodomain and extra-terminal inhibitor JQ1 to down-regulate the expression of PD-L1 and thus elicit the immune response to TNBC instead of using antibodies to block PD-L1. JQ1 also inhibits the growth of TNBC as a targeted therapeutic agent by inhibiting the BRD4-c-MYC axis. The polydopamine nanoparticles (PDMNs) are introduced as a biodegradable and adaptable platform to load JQ1 and induce photothermal therapy (PTT) as another synergistic therapeutic modality. Because the JQ1-loaded PDMNs (PDMN-JQ1) are self-degradable and release JQ1 continuously, this synergistic treatment can lead to remarkable activation of cytotoxic T lymphocytes and induce a strong immune-memory effect to protect mice from tumor re-challenge. Taken together, our study demonstrates a compact and simple nanoplatform for triple therapy, including targeted therapy, PTT, and immunotherapy, for TNBC treatment.
Assuntos
Antígeno B7-H1/genética , Nanopartículas/química , Fototerapia , Proteínas Proto-Oncogênicas c-myc/genética , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Animais , Anticorpos/genética , Apoptose/efeitos dos fármacos , Azepinas/química , Azepinas/farmacologia , Antígeno B7-H1/antagonistas & inibidores , Proteínas de Ciclo Celular/antagonistas & inibidores , Proteínas de Ciclo Celular/genética , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Xenoenxertos , Humanos , Indóis/química , Indóis/farmacologia , Terapia com Luz de Baixa Intensidade , Camundongos , Polímeros/química , Polímeros/farmacologia , Proteínas Proto-Oncogênicas c-myc/antagonistas & inibidores , Fatores de Transcrição/antagonistas & inibidores , Fatores de Transcrição/genética , Triazóis/química , Triazóis/farmacologia , Neoplasias de Mama Triplo Negativas/genética , Neoplasias de Mama Triplo Negativas/patologiaRESUMO
B-1 cells are a unique subset of B cells that are positively selected for expressing autoreactive BCRs. We isolated RNA from peritoneal (B-1a, B-1b, B-2) and splenic (B-1a, marginal zone, follicular) B cells from C57BL/6 mice and used 5'-RACE to amplify the IgH V region using massively parallel sequencing. By analyzing 379,000 functional transcripts, we demonstrate that B-1a cells use a distinct and restricted repertoire. All B-1 cell subsets, especially peritoneal B-1a cells, had a high proportion of sequences without N additions, suggesting predominantly prenatal development. Their transcripts differed markedly and uniquely contained VH11 and VH12 genes, which were rearranged only with a restricted selection of D and J genes, unlike other V genes. Compared to peritoneal B-1a, the peritoneal B-1b repertoire was larger, had little overlap with B-1a, and most sequences contained N additions. Similarly, the splenic B-1a repertoire differed from peritoneal B-1a sequences, having more unique sequences and more frequent N additions, suggesting influx of B-1a cells into the spleen from nonperitoneal sites. Two CDR3s, previously described as Abs to bromelain-treated RBCs, comprised 43% of peritoneal B-1a sequences. We show that a single-chain variable fragment designed after the most prevalent B-1a sequence bound oxidation-specific epitopes such as the phosphocholine of oxidized phospholipids. In summary, we provide the IgH V region library of six murine B cell subsets, including, to our knowledge for the first time, a comparison between B-1a and B-1b cells, and we highlight qualities of B-1 cell Abs that indicate unique selection processes.
Assuntos
Anticorpos/genética , Anticorpos/imunologia , Subpopulações de Linfócitos B/imunologia , Baço/imunologia , Sequência de Aminoácidos , Animais , Diversidade de Anticorpos/genética , Diversidade de Anticorpos/imunologia , Sequência de Bases , Feminino , Genes de Imunoglobulinas/genética , Genes de Imunoglobulinas/imunologia , Cadeias Pesadas de Imunoglobulinas/genética , Cadeias Pesadas de Imunoglobulinas/imunologia , Região Variável de Imunoglobulina/genética , Região Variável de Imunoglobulina/imunologia , Camundongos , Camundongos Endogâmicos C57BLRESUMO
The production of therapeutic antibodies using mammalian cells remains a high-priority in the biopharmaceutical manufacturing industry. Bioengineers have targeted different cellular processes, including transcription, translation, secretion and post-translational modifications, to overcome the metabolic bottlenecks limiting production capacity and create high-producing mammalian cell lines. The polycomb group (PcG) proteins belong to a family of chromatin regulators with important roles in multicellular development. By overexpressing and screening genes from the PcG family, we have identified an epigenetic key player for biopharmaceutical manufacturing enhancement: the transcription factor Yin Yang 1 (YY1). The overexpression of YY1 led to an increase in the production of several product genes (SEAP, VEGF165, IgG including Rituximab), provided that human YY1 (hYY1) was expressed in human cells (HeLa, HT-1080, HEK-293T, FreeStyle™ 293-F) and Chinese hamster ovary cell-derived YY1 (cYY1) was expressed in CHO cells (CHO-K1, CHO-easyC, FreeStyle™ CHO-S, CHO-B13-24, CHO-IgG1). Ectopic expression of cYY1 in the stable CHO-derived IgG producer cell lines CHO-B13-24 and CHO-IgG1 increased the antibody titer up to 6-fold, suggesting that epigenetic engineering of mammalian production cell lines could become a new strategy to improve the manufacturing of complex protein pharmaceuticals.
Assuntos
Anticorpos/metabolismo , Expressão Gênica , Engenharia de Proteínas/métodos , Fator de Transcrição YY1/genética , Animais , Anticorpos/genética , Células CHO , Cricetulus , Epigênese Genética , Células HeLa , HumanosRESUMO
Hapten antibodies are active components of traditional Chinese medicines, have been widely applied in all of study fields of traditional Chinese medicine. First, hapten monoclonal antibodies could be designed into ELISA kits for quantitative analysis on the content of effective components in plant crude extracts or biological samples, which be applied for quality control and studies on pharmacokenetics of traditional Chinese medicines. Second, hapten monoclonal antibodies could be coupled with solid-phase carriers to generate immunoaffinity chromatography column, which could be used for knock-out extract preparation or pre-treatment of complicated sampless. Finally, a single-chain variable fragment antibody (scFV) gene segment of effective components of hapten monoclonal antibodies could be transformed into relative plant cells to gain new varieties with high-enrichment effective components, and thus achieve the molecular breeding of medicinal plants.
Assuntos
Anticorpos , Haptenos , Medicina Tradicional Chinesa/métodos , Animais , Anticorpos/genética , Anticorpos/imunologia , Cromatografia de Afinidade , Ensaio de Imunoadsorção Enzimática , Técnicas de Inativação de Genes , Haptenos/imunologia , Haptenos/metabolismo , HumanosRESUMO
Hypericum perforatum is well known for its antidepressant and anti-inflammatory activities, for which hypericin and its derivatives are indicated to be the most active compounds. Hypericin synthase (Hyp-1) is the only protein proven to catalyze the synthesis of hypericin. In this study, the full-length cDNA of Hyp-1 was chemically synthesized according to the Hyp-1 sequence in GenBank (accession no. AY148090) and then cloned into the plasmid pET22b. Hyp-1 was expressed in Escherichia coli BL21 (DE3) and purified with a Ni-NTA column. The purified protein was used to immunize New Zealand white rabbits, from which an antiserum was purified by protein G affinity chromatography. The polyclonal antibody against Hyp-1 provides a valuable tool for the study of hypericin biosynthesis in H. perforatum. Expression of Hyp-1 and the cellular distribution of hypericin were analyzed in different organs of red-pigmented H. perforatum plantlets. The black glands were not the only site of hypericin accumulation and the results indicated that hypericin might be synthesized in mesophyll cells or in tissues of the root and/or stem and then transported to the glands. This work provides a foundation for further investigation of the regulatory mechanism of hypericin synthesis during the development of H. perforatum.
Assuntos
Anticorpos , Peptídeo Sintases , Perileno/análogos & derivados , Extratos Vegetais , Animais , Antracenos , Anticorpos/genética , Anticorpos/imunologia , Anticorpos/metabolismo , Antidepressivos/imunologia , Antidepressivos/metabolismo , Antidepressivos/farmacologia , Hypericum/química , Hypericum/metabolismo , Peptídeo Sintases/imunologia , Peptídeo Sintases/isolamento & purificação , Perileno/imunologia , Perileno/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Caules de Planta/enzimologia , Caules de Planta/imunologia , CoelhosRESUMO
The degradation of proteins by asparagine deamidation and aspartate isomerization is one of several chemical degradation pathways for recombinant antibodies. In this study, we have identified two solvent accessible degradation sites (light chain aspartate-56 and heavy chain aspartate-99/101) in the complementary-determining regions of a recombinant IgG1 antibody susceptible to isomerization under elevated temperature conditions. For both hot-spots, the degree of isomerization was found to be significantly higher than the deamidation of asparagine-(387, 392, 393) in the conserved CH3 region, which has been identified as being solvent accessible and sensitive to chemical degradation in previous studies. In order to reduce the time for simultaneous identification and functional evaluation of potential asparagine deamidation and aspartate isomerization sites, a test system employing accelerated temperature conditions and proteolytic peptide mapping combined with quantitative UPLC-MS was developed. This method occupies the formulation buffer system histidine/HCl (20 mM; pH 6.0) for denaturation/reduction/digestion and eliminates the alkylation step. The achieved degree of asparagine deamidation and aspartate isomerization was adequate to identify the functional consequence by binding studies. In summary, the here presented approach greatly facilitates the evaluation of fermentation, purification, formulation, and storage conditions on antibody asparagine deamidation and aspartate isomerization by monitoring susceptible marker peptides located in the complementary-determining regions of recombinant antibodies.
Assuntos
Anticorpos/metabolismo , Asparagina/metabolismo , Ácido Aspártico/metabolismo , Cromatografia Líquida/métodos , Espectrometria de Massas/métodos , Amidas/química , Amidas/metabolismo , Sequência de Aminoácidos , Animais , Anticorpos/química , Anticorpos/genética , Asparagina/química , Ácido Aspártico/química , Células CHO , Regiões Determinantes de Complementaridade/química , Regiões Determinantes de Complementaridade/genética , Regiões Determinantes de Complementaridade/metabolismo , Cricetinae , Cricetulus , Concentração de Íons de Hidrogênio , Imunoglobulina G/química , Imunoglobulina G/genética , Imunoglobulina G/metabolismo , Isomerismo , Ligação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Ressonância de Plasmônio de Superfície , TemperaturaRESUMO
The large-scale production of plant-derived recombinant proteins requires the breeding of lines homozygous for the transgene(s). These can be selected by progeny testing over multiple sexual generations, but a more efficient means is to fix homozygosity in a single generation using doubled haploid technology. In this study, transgenic tobacco plants, hemizygous for both of the independently inherited genes encoding the light and heavy chains of the anti-human immunodeficiency virus monoclonal antibody 2F5, were used to establish embryogenic pollen cultures. The improved protocol employed in this study guaranteed a very high regeneration efficiency, with more than 50% of the regenerants being spontaneously doubled haploids. Hence, there was no requirement to chemically induce chromosome doubling to recover sufficient entirely homozygous recombinants. As expected, approximately 25% of the regenerants were homozygous for both transgenes. Thus, the employment of haploid technology allowed for the efficient and rapid generation of true-breeding tobacco lines accumulating functional immunoglobulins.
Assuntos
Anticorpos/metabolismo , Haploidia , Nicotiana/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Anticorpos/genética , Western Blotting , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Regulação da Expressão Gênica de Plantas , Plantas Geneticamente Modificadas/genética , Pólen/citologia , Pólen/embriologia , Reação em Cadeia da Polimerase , Nicotiana/genéticaRESUMO
A diarréia é um importante problema de saúde pública no mundo inteiro e a Escherichía coli é um dos mais freqüentes microorganismos causadores desta doença. A Escherichia coli enteropatogênica (EPEC), um dos principais agentes etiológicos das diarréias infantis no nosso país, é genética e fenotipicamente relacionada com a E. colí enterohemorrágica (EHEC) que além de provocar diarréia é responsável por complicações como síndrome hemolítica urêmica (HUS) e colite hemorrágica (HC). Embora a EHEC seja considerada emergente pela OMS, no Brasil poucos casos de complicações como HUS e HC foram reportados. O mecanismo de patogenicidade comum entre EPEC e EHEC é conhecido como a lesão "attaching and effacing" nos microvilos do enterócito. Esta lesão é mediada por um conjunto de fatores de virulência, dentre eles a intimina. A intimina é uma proteína de membrana externa, responsável pelo íntimo contato da bactéria com o enterócito, possui uma região N-terminal que é altamente conservada e uma região C-terminal que é variável. De acordo com a região variável, existem vários subtipos de intimina, dentre eles as intiminas , α, β e γ...
Assuntos
Humanos , Masculino , Feminino , Adulto , Anticorpos/genética , Anticorpos/imunologia , Diarreia/genética , Diarreia/imunologia , Escherichia coli Enteropatogênica/fisiologia , Escherichia coli Enteropatogênica/imunologia , Fatores de Virulência/genética , Fatores de Virulência/imunologia , Infecções por Escherichia coli/imunologia , Colostro , Ensaio de Imunoadsorção Enzimática , Métodos Analíticos de Preparação de Amostras , Soro , Interpretação Estatística de DadosRESUMO
Profilins are highly cross-reactive allergens in pollens and plant food. In a paradigmatic approach, the cDNA coding for timothy grass pollen profilin, Phl p 12, was used as a template to develop a new strategy for engineering an allergy vaccine with low IgE reactivity. Non-IgE-reactive fragments of Phl p 12 were identified by synthetic peptide chemistry and restructured (rs) as a new molecule, Phl p 12-rs. It comprised the C terminus of Phl p 12 at its N terminus and the Phl p 12 N terminus at its C terminus. Phl p 12-rs was expressed in Escherichia coli and purified to homogeneity. Determination of secondary structure by circular dichroism indicated that the restructuring process had reduced the IgE-reactive alpha-helical contents of the protein but retained its beta-sheet conformation. Phl p 12-rs exhibited reduced IgE binding capacity and allergenic activity but preserved T cell reactivity in allergic patients. IgG Abs induced by immunization of mice and rabbits with Phl p 12-rs cross-reacted with pollen and food-derived profilins. Recombinant Phl p 12-rs, rPhl p 12-rs, induced less reaginic IgE to the wild-type allergen than rPhl p 12. However, the rPhl p 12-rs-induced IgGs inhibited allergic patients' IgE Ab binding to profilins to a similar degree as those induced by immunization with the wild type. Phl p 12-rs specific IgG inhibited profilin-induced basophil degranulation. In conclusion, a restructured recombinant vaccine was developed for the treatment of profilin-allergic patients. The strategy of tail-to-head reassembly of hypoallergenic allergen fragments within one molecule represents a generally applicable strategy for the generation of allergy vaccines.
Assuntos
Alérgenos/imunologia , Antialérgicos/imunologia , Antígenos de Plantas/imunologia , Pólen/imunologia , Profilinas/imunologia , Proteínas Recombinantes de Fusão/imunologia , Vacinas/imunologia , Alérgenos/química , Alérgenos/genética , Antialérgicos/química , Anticorpos/química , Anticorpos/genética , Anticorpos/imunologia , Especificidade de Anticorpos/genética , Especificidade de Anticorpos/imunologia , Reações Antígeno-Anticorpo , Antígenos de Plantas/química , Antígenos de Plantas/genética , Sítios de Ligação , Dicroísmo Circular , Epitopos/imunologia , Engenharia Genética/métodos , Histamina/imunologia , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Modelos Moleculares , Pólen/química , Pólen/genética , Reação em Cadeia da Polimerase , Profilinas/química , Profilinas/genética , Estrutura Secundária de Proteína , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Sensibilidade e Especificidade , Linfócitos T/imunologia , Vacinas/química , Vacinas/genéticaRESUMO
Proteomics is hungry for well-validated antibodies. Nathan Blow looks at the options and sees how researchers are redefining the way to generate an antibody.
Assuntos
Anticorpos/imunologia , Anticorpos/isolamento & purificação , Avaliação Pré-Clínica de Medicamentos/métodos , Imunoensaio/normas , Proteômica/métodos , Animais , Anticorpos/genética , Afinidade de Anticorpos , Citometria de Fluxo , Humanos , Imunoensaio/métodos , Indicadores e Reagentes/isolamento & purificação , Indicadores e Reagentes/normas , Proteoma , Proteômica/normas , Controle de Qualidade , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/normas , Reprodutibilidade dos TestesAssuntos
Anticorpos/imunologia , Anticorpos/isolamento & purificação , Avaliação Pré-Clínica de Medicamentos/métodos , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/normas , Animais , Anticorpos/genética , Afinidade de Anticorpos , Especificidade de Anticorpos , Citometria de Fluxo , Fosforilação , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genéticaRESUMO
To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a full-length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The alphaSIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the alpha-CH3 domain from human IgA. To express the full-length IgA, the individual light and heavy chains from the TGEV-specific mAb 6A.C3 were inserted into separate PVX constructs and plants were co-infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plant-expressed alphaSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibody-expressing plant tissue to 2-day-old piglets showed that both the alphaSIP and full-length IgA molecules can provide in vivo protection against TGEV.
Assuntos
Anticorpos/imunologia , Comovirus/genética , Coronavirus/imunologia , Imunoglobulina A/imunologia , Imunoglobulina A/metabolismo , Proteínas de Plantas/imunologia , Potexvirus/genética , Animais , Anticorpos/genética , Anticorpos/metabolismo , Vetores Genéticos/genética , Imunoglobulina A/genética , Região Variável de Imunoglobulina/imunologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Engenharia de Proteínas/métodos , Proteínas Recombinantes/metabolismo , Suínos , Transfecção/métodosRESUMO
We previously reported that during mouse embryogenesis, plexin D1 (plxnD1) is expressed on neuronal and endothelial cells. Endothelial cells gradually loose plxnD1 expression during development. Here we describe, using in situ hybridization, that endothelial plxnD1 expression is regained during tumor angiogenesis in a mouse model of brain metastasis. Importantly, we found PLXND1 expression also in a number of human brain tumors, both of primary and metastatic origin. Apart from the tumor vasculature, abundant expression was also found on tumor cells. Via panning of a phage display library, we isolated two phages that carry single-domain antibodies with specific affinity towards a PLXND1-specific peptide. Immunohistochemistry with these single-domain antibodies on the same tumors that were used for in situ hybridization confirmed PLXND1 expression on the protein level. Furthermore, both these phages and the derived antibodies specifically homed to vessels in brain lesions of angiogenic melanoma in mice after i.v. injection. These results show that PLXND1 is a clinically relevant marker of tumor vasculature that can be targeted via i.v. injections.
Assuntos
Neoplasias Encefálicas/irrigação sanguínea , Neoplasias Encefálicas/genética , Moléculas de Adesão Celular Neuronais/biossíntese , Glicoproteínas de Membrana/biossíntese , Proteínas do Tecido Nervoso/biossíntese , Sequência de Aminoácidos , Animais , Anticorpos/genética , Anticorpos/imunologia , Anticorpos/isolamento & purificação , Especificidade de Anticorpos , Bacteriófagos/genética , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/terapia , Camelídeos Americanos , Moléculas de Adesão Celular Neuronais/genética , Humanos , Imuno-Histoquímica , Hibridização In Situ , Peptídeos e Proteínas de Sinalização Intracelular , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , Neovascularização Patológica/genética , Neovascularização Patológica/metabolismo , Neovascularização Patológica/terapia , Proteínas do Tecido Nervoso/genética , RNA/biossíntese , RNA/genéticaRESUMO
Within the biomedical and pharmaceutical communities there is an ongoing need to find new technologies that can be used to elucidate disease mechanisms and provide novel therapeutics. Antibodies are arguably the most powerful tools in biomedical research, and antibodies specific for extracellular or cell-surface targets are currently the fastest growing class of new therapeutic molecules. However, the majority of potential therapeutic targets are intracellular, and antibodies cannot readily be leveraged against such molecules, in the context of a viable cell or organism, because of the inability of most antibodies to form stable structures in an intracellular environment. Advances in recent years, in particular the development of intracellular screening protocols and the definition of antibody structures that retain their antigen-binding function in an intracellular context, have allowed the robust isolation of a subset of antibodies that can function in an intracellular environment. These antibodies, generally referred to as intrabodies, have immense potential in the process of drug development and may ultimately become therapeutic entities in their own right.
Assuntos
Anticorpos/imunologia , Anticorpos/uso terapêutico , Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos/métodos , Espaço Intracelular/imunologia , Animais , Anticorpos/genética , Humanos , Engenharia de ProteínasRESUMO
We describe a novel approach for high-throughput screening of recombinant antibodies, based on their immobilization on solid cellulose-based supports. We constructed a large human synthetic single-chain Fv antibody library where in vivo formed complementarity determining regions were shuffled combinatorially onto germline-derived human variable-region frameworks. The arraying of library-derived scFvs was facilitated by our unique display/expression system, where scFvs are expressed as fusion proteins with a cellulose-binding domain (CBD). Escherichia coli cells expressing library-derived scFv-CBDs are grown on a porous master filter on top of a second cellulose-based filter that captures the antibodies secreted by the bacteria. The cellulose filter is probed with labeled antigen allowing the identification of specific binders and the recovery of the original bacterial clones from the master filter. These filters may be simultaneously probed with a number of antigens allowing the isolation of a number of binding specificities and the validation of specificity of binders. We screened the library against a number of cancer-related peptides, proteins, and peptide-protein complexes and yielded antibody fragments exhibiting dissociation constants in the low nanomolar range. We expect our new antibody phage library to become a valuable source of antibodies to many different targets, and to play a vital role in facilitating high-throughput target discovery and validation in the area of functional cancer genomics.
Assuntos
Anticorpos/imunologia , Regiões Determinantes de Complementaridade/imunologia , Biblioteca Gênica , Fragmentos de Imunoglobulinas/imunologia , Região Variável de Imunoglobulina/imunologia , Anticorpos/genética , Antígenos de Neoplasias/imunologia , Celulose/química , Técnicas de Química Combinatória , Regiões Determinantes de Complementaridade/genética , Avaliação Pré-Clínica de Medicamentos/métodos , Escherichia coli/genética , Humanos , Immunoblotting , Fragmentos de Imunoglobulinas/genética , Região Variável de Imunoglobulina/genética , Ligação Proteica , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/imunologiaAssuntos
Anticorpos/análise , Anticorpos/metabolismo , Desenho de Fármacos , Biblioteca de Peptídeos , Engenharia de Proteínas/métodos , Proteômica/métodos , Robótica/métodos , Anticorpos/genética , Clonagem Molecular/métodos , Avaliação Pré-Clínica de Medicamentos/métodos , Engenharia de Proteínas/tendências , Mapeamento de Interação de Proteínas/métodos , Proteômica/tendências , Proteínas Recombinantes/análise , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Robótica/tendências , Integração de SistemasRESUMO
We constructed a recombinant antibody fragment--single chain fragment-variable (scFv) antibody--derived from hybridoma cell lines to control the concentration of solasodine glycosides in hairy root cultures of Solanum khasianum transformed by the anti-solamargine (As)-scFv gene. The properties of the As-scFv protein expressed in Escherichia coli were almost identical to those of the parent monoclonal antibody (MAb). Up to 220 ng recombinant As-scFv was expressed per milligram of soluble protein in transgenic hairy root cultures of S. khasianum. The concentration of solasodine glycosides was 2.3-fold higher in the transgenic than in the wild-type hairy root, as reflected by the soluble As-scFv level and antigen binding activities. These results suggested that the scFv antibody expressed in transgenic hairy roots controlled the antigen level, thus representing a novel plant breeding methodology that can produce secondary metabolites.