Accurate, automatic determination of astigmatism and phase with Ctfplotter in IMOD.

Ctfplotter in the IMOD software package is a flexible program for determination of CTF parameters in tilt series images. It uses a novel approach to find astigmatism by measuring defocus in one-dimensional power spectra rotationally averaged over a series of restricted angular ranges. Comparisons with Ctffind, Gctf, and Warp show that Ctfplotter's estimated astigmatism is generally more reliable than that found by these programs that fit CTF parameters to two-dimensional power spectra, especially at higher tilt angles. In addition to that intrinsic advantage, Ctfplotter can reduce the variability in astigmatism estimates further by summing results over multiple tilt angles (typically 5), while still finding defocus for each individual image. Its fitting strategy also produces better phase estimates. The program now includes features for tuning the sampling of the power spectrum so that it is well-represented for analysis, and for determining an appropriate fitting range that can vary with tilt angle. It can thus be used automatically in a variety of situations, not just for fitting tilt series, and has been integrated into the SerialEM acquisition software for real-time determination of focus and astigmatism.

Minimum Corneal Diameter and Anterior Steep Axis Curvature Share the Same Meridian: A Novel Finding.

PURPOSE: To define the external scleral sulcus (ESS) on a Scheimpflug image and use it for a morphometric analysis of corneal diameter (CD). DESIGN: Retrospective, cross-sectional study of pediatric Asian-Indian eyes. METHODS: One random eye of 353 subjects between 5 and 18 years underwent 25-scan Pentacam HR imaging. For all scans, densitometry values along the anterior corneal edge were recorded and differentiated. The peaks on the differentiated curve were chosen as the ESS points, and this distance between them was called CD. Vertical (vCD), maximum (maxCD), minimum (minCD) CD and their meridians were defined. Multiple regression models (MRMs) with CD and other Pentacam parameters were built to predict astigmatism and its axis, mean keratometry (Kmean), and Belin/Ambrósio enhanced ectasia display deviation (BAD-D). MRMs were validated using intraclass correlation coefficient (ICC). Estimated horizontal CD (hCD) was validated against digital caliper measurement using ICC. RESULTS: The ICC (95% CI) between caliper and hCD was 0.96 (0.93, 0.97). MRM predictions (P < .001) used CD parameters, anterior chamber depth, corneal volume and distance from the corneal thinnest location to apex. These predictions achieved an ICC of 0.34 (0.18, 0.46), 0.82 (0.78, 0.86), 0.87 (0.84, 0.89), and 0.81 (0.76, 0.84), respectively. The astigmatism axis prediction depended on the minCD and maxCD meridians. Its within-subject SD (4.97°) was less than 2 consecutive Pentacam scan angles (7.2°). CONCLUSIONS: The CD metric strongly correlated with the astigmatism axis, keratometry, and BAD-D. Its spatial description may be significant in corneal treatment planning and disease diagnoses.

Short-term effects on ocular variables immediately after hyperbaric oxygen exposures.

PURPOSE: To determine ocular refraction, corneal thickness, corneal radius, corneal power, corneal astigmatism and intraocular pressure in patients before and immediately after repeated hyperbaric oxygen (HBO2) exposures twice a week during six weeks of HBO2 therapy. METHODS: 23 patients received HBO2 therapy at 2.4 ATA for 90 minutes daily in monoplace chambers for six weeks, five days a week. The Topcon TRK-1P instrument was installed next to the hyperbaric chambers to record the ocular measurements. RESULTS: A gross myopic shift developed at -0.95 ± 0.54 D (P ⟨ 0.001) in the right eye and -0.95 ± 0.53 D (P ⟨ 0.001) in the left eye during the six weeks of treatment. Myopic shift reversion, corneal thinning and reduced intraocular pressure appeared as immediate effects after a single HBO2 exposure, but resolved before the patients attended for the next measurement visit. CONCLUSIONS: Ocular variables were influenced by both cumulative and transient short-term effects during the HBO2 therapy. The short-term effects showed that the point of time for performing the ocular measurements after HBO2 exposure might influence the result and must be considered before making relevant comparisons among studies.

Refractive status of Chinese with laser-treated retinopathy of prematurity.

PURPOSE: To analyze changes in myopia, astigmatism, and anisometropia after laser treatment of retinopathy of prematurity (ROP), including aggressive posterior retinopathy of prematurity (APROP), in Mainland Chinese children. METHODS: This was a retrospective study of children who had laser treatment for threshold or type 1 prethreshold ROP between January 2004 and October 2012 and age-matched control subjects with spontaneously regressed type 2 prethreshold ROP. One hundred fifteen eyes of 60 patients were included as the laser-treated group, which were further subdivided into APROP and non-APROP groups. Thirty-seven eyes of 20 patients who were diagnosed during the same period were included as the control group. Between 12 and 36 months postnatal age (PA) (mean [±SD], 22.9 [±8.1] months), cycloplegic retinoscopy was performed to measure refractive outcomes. A general linear model was used to analyze refractive changes among different groups at each PA. RESULTS: After adjusting for PA and the correlation between right and left eyes, the magnitude and proportion of astigmatism (p = 0.04 and p = 0.004, respectively) and myopia (p < 0.0001 and p = 0.006, respectively) were greater in the laser-treated group than in the control group. The differences in myopia were even greater in children with APROP than those with non-APROP, whereas the differences in astigmatism were not. Eyes with APROP had higher prevalence of high myopia and spherical anisometropia than the control (p = 0.002 and p = 0.02, respectively) and the non-APROP groups (p < 0.0001 and p = 0.04, respectively). CONCLUSIONS: Children with laser treatment for ROP, including APROP, tended to have higher myopia, astigmatism, and anisometropia, which may progress to amblyopia. These findings highlight the need for regular refractive screening after laser treatment of ROP.

Combined corneal topography and corneal wavefront data in the treatment of corneal irregularity and refractive error in LASIK or PRK using the Carl Zeiss Meditec MEL 80 and CRS-Master.

PURPOSE: To assess the efficacy, safety, and accuracy of TOSCA II (Topography Supported Custom Ablation) treatments for the correction of high corneal aberrations and refractive error following previous ocular surgery. METHODS: Forty-eight eyes of 32 patients were included for study. Indications for TOSCA II treatment included small optical zone, decentered ablation, subjective visually significant higher order aberrations, and irregularly irregular astigmatism induced by LASIK, photorefractive keratectomy, radial keratotomy, conductive keratoplasty, cataract surgery, and deep lamellar keratoplasty. An ablation profile based on corneal topography data and corneal wavefront and manifest refraction was created using the CRS-Master software (Carl Zeiss Meditec) to treat corneal irregularity and refractive error simultaneously. Postoperative assessments were performed at 1 week and 1, 2, 3, 6, and 12 months. RESULTS: Mean attempted spherical equivalent refraction (SE) was -1.12 +/- 1.97 diopters (D) (range: +1.13 to -10.50 D). Mean attempted cylinder correction was 1.34 +/- 1.65 D (range: 0.00 to 7.75 D). Median age was 38 years (range: 19 to 68 years). Median follow-up was 9.4 months. Accuracy: mean deviation from the intended SE correction was -0.19 +/- 0.94 D, 60% of eyes were within +/- 0.50 D and 77% of eyes were within +/- 1.00 D of the intended SE. Cylinder: the correction ratio was 1.49 +/- 0.85 and the error ratio was 0.87 +/- 0.69. EFFICACY: 56% of eyes achieved uncorrected visual acuity of 20/20 or better and 88% achieved 20/40 or better. SAFETY: 34% of eyes gained one or more lines of best spectacle-corrected visual acuity, 15% lost one line, and no eye lost two or more lines. Contrast sensitivity: for the subset of eyes with below normal contrast sensitivity preoperatively, there was a statistically significant improvement in contrast sensitivity at 3 cycles per degree (cpd), 6 cpd, and 12 cpd (P < .05). Stability: the average change in refraction between 3 months and 1year was +0.38 +/- 0.30 D. Higher order aberrations: the average higher order root-mean-square decreased by 21% and the average spherical aberration decreased by 41%. CONCLUSIONS: The MEL 80 CRS-Master TOSCA II software appears to be an effective treatment for decentrations, optical zone enlargement, and reduction of higher order aberrations. The refractive outcome was satisfactory after one treatment.

Early clinical evaluation of AcrySof ReSTOR multifocal intraocular lens for treatment of cataract.

PURPOSE: To evaluate the efficacy, safety and early visual quality of the AcrySof ReSTOR multifocal intraocular lens (IOL) following cataract surgery. SETTING: Eye Center of Shandong University of Traditional Chinese Medicine, Jinan Shierming Eye Hospital, Jinan, Shandong, China. METHODS: After small-incision phacoemulsification, AcrySof ReSTOR IOLs were implanted in 20 subjects (40 eyes, ReSTOR group). Monofocal IOLs were implanted in 18 subjects (36 eyes, control group). Three to six months following bilateral implantation, visual acuity, depth of focus, corneal astigmatism, contrast sensitivity, glare sensitivity, visual field and spherical aberration were compared between the groups. A subjective outcome questionnaire was also completed by the subjects. RESULTS: Uncorrected near visual acuity 0.5 or better was achieved by 92.5% of the ReSTOR eyes. In the monofocal eyes, only 33.3% of the eyes achieved uncorrected near visual acuity of 0.5 or better. There was no statistical difference between corrected near, distance visual acuity, and uncorrected distance visual acuity in the 2 groups (p > 0.05). In patients with visual acuity 0.5 or better, the depth of focus was 4.87 +/- 1.09 and 2.08 +/- 0.69 dpt in the ReSTOR and control groups, respectively. The contrast sensitivity, glare sensitivity and visual field of both groups were similar (p > 0.05). The spherical aberration of the 2 groups was similar (t = -0.37, p = 0.71). Visual disturbances were minimal with only 1 patient in each group complaining of glare at night. CONCLUSION: The AcrySof ReSTOR multifocal IOL is a safe and effective treatment for cataracts.