RESUMO
Human sperm banking is an important procedure in the assisted reproductive technique centers. It entails sperm damage. The aim of this study was to investigate beneficial effect of Ceratonia siliqua (C. siliqua) supplement in freezing/thawing media on post thaw sperm parameters and sperm chromatin quality in normozoospermic samples. Forty normozoospermic specimens were included in this prospective study. Each sample was divided into ten groups. In groups one to five, 0 (as control group) 5, 10, 20 and 30 µg/ml C. siliqua were added to freezing medium and in groups six to ten, similar concentration of C. siliqua were added to thawing medium for 30 min incubation. Sperm concentration, progressive motility, normal morphology, viability, aniline blue (AB), toluidine blue (TB) and sperm chromatin dispersion (SCD) staining tests were evaluated before vitrification and after thawing. The results showed that 10 and 20 µg/ml supplementation of C. siliqua in freezing/thawing media significantly increased progressive motility, normal morphology and viability of sperm (p < 0.05) as well as decreased AB, TB and SCD (p < 0.05). Also, 20 µg/ml had significantly higher improvement compared to 10 µg/ml C. siliqua (p < 0.05). The present study showed that C. siliqua supplemented freezing/thawing media can improve sperm quality of normozoospermic samples after freezing/thawing.
Assuntos
Cromatina/metabolismo , Criopreservação/instrumentação , Fabaceae/química , Extratos Vegetais/farmacologia , Preservação do Sêmen/instrumentação , Espermatozoides/efeitos dos fármacos , Espermatozoides/patologia , Adulto , Antioxidantes/química , Criopreservação/métodos , Congelamento , Humanos , Masculino , Estudos Prospectivos , Análise do Sêmen , Preservação do Sêmen/métodos , Bancos de Esperma , Contagem de Espermatozoides , Motilidade dos Espermatozoides , Temperatura , VitrificaçãoAssuntos
Atenção à Saúde , Rádio , Televisão , Serviço Hospitalar de Emergência , Hospitais , Humanos , Bancos de Esperma , Fatores de Tempo , Reino Unido , YogaRESUMO
Sperm cryopreservation is the only method currently available that offers men with cancer insurance against sterilising iatrogenic treatments. We carried out two cohort and cross-sectional audits to identify trends with sperm cryopreservation referral rates and sample usage rates for men diagnosed with cancer and who banked sperm at The Andrology Laboratory, Hammersmith Hospital, Imperial College NHS Trust. These retrospective audits revealed that a total of 4362 men with cancer successfully banked sperm between 1976 and 2013. Truncating the dataset to 2009 to allow for lag times between storage and use, the overall sample usage rate for cancer patients was 6.0% with 75 live births. Increased median age at referral influences the cancer profile of men seen at the bank, which is highlighted by a disproportionate rise in the number of men with prostate cancer. Among men who use banked sperm, a large rise in the use of intracytoplasmic sperm injection has occurred over time. The number of patients requiring the service is sharply increasing year on year as are the number of patients who go on to use their sample in assisted conception. The historical use rates of frozen sperm are likely to be significant underestimations of future use.
Assuntos
Criopreservação/tendências , Preservação da Fertilidade/tendências , Neoplasias da Próstata/terapia , Preservação do Sêmen/tendências , Bancos de Esperma/tendências , Adolescente , Adulto , Idoso , Estudos de Coortes , Estudos Transversais , Criopreservação/métodos , Criopreservação/estatística & dados numéricos , Preservação da Fertilidade/métodos , Preservação da Fertilidade/estatística & dados numéricos , Humanos , Londres , Masculino , Pessoa de Meia-Idade , Programas Nacionais de Saúde , Técnicas de Reprodução Assistida , Estudos Retrospectivos , Preservação do Sêmen/métodos , Preservação do Sêmen/estatística & dados numéricos , Bancos de Esperma/métodos , Bancos de Esperma/estatística & dados numéricos , Estatísticas não Paramétricas , Adulto JovemRESUMO
Drug treatment for erectile dysfunction is sometimes effective.The efficacy of pelvic floor muscle training on post-surgical urinary incontinence is uncertain.