RESUMO
The relationship between virulence and encapsulation of Aerococcus viridans var. homari was evaluated by growing virulent (Rabin's) and avirulent (ATCC 10400) strains under varying culture conditions, and during challenge trials. Changes in capsule thickness were monitored using a modified lysine-ruthenium red (LRR) fixation method and transmission electron microscopy. The virulent Rabin's strain possessed a prominent capsule of 0.252 µm±0.061 µm that was diminished by in vitro growth conditions to 0.206 µm±0.076 µm. The ATCC 10400 strain capsule thickness decreased from 0.157 µm±0.043 µm to 0.117 µm±0.043 µm after 10 in vitro passages. The virulent Rabin's strain capsule was significantly thicker than the avirulent ATCC 10400 strain under all growth conditions. Rabin's strain, regardless of pre-challenge growth conditions or dose (high dose 10(7) or low dose 10(2)), was able to kill lobsters in 7 days at 15°C. ATCC 10400 strain, regardless of pre-challenge growth conditions, killed lobster only at high doses (10(7)) with varying median time to death of â¼15 days, while at low doses (10(2)) all lobsters survived and no bacteria were present after 42 days. This work demonstrates the importance of the thickness of the A. viridans capsule to virulence in the American lobster.
Assuntos
Aerococcus/patogenicidade , Cápsulas Bacterianas/fisiologia , Nephropidae/microbiologia , Aerococcus/ultraestrutura , Animais , Resistência à Doença , Interações Hospedeiro-Patógeno , Nephropidae/imunologia , VirulênciaRESUMO
Virulent and avirulent strains of Aerococcus viridans (var.) homari were used to extend previous studies to determine and confirm differences between the 2 types. Virulent strains possessed polysaccharide capsules and were not agglutinated by lobster hemolymph serum; avirulent strains did not have capsules, were agglutinated by the lobster hemolymph serum, and most did not grow well in lobster hemolymph serum. Growth of the avirulent strains in sterile lobster hemolymph serum induced the production of capsules (which reached a maximum after 5 to 7 d incubation), eliminated susceptibility of the strains to the lobster serum agglutinin, and restored their virulence against lobsters. The factor(s) in lobster hemolymph serum inducing the long-lasting phenotypic response of virulence was (were) heat labile.