RESUMO
The present study aimed to investigate the effects of Solanum nigrum Linne (SNL) in a model of 1chloro2,4dinitrobenzene (DNCB)induced atopic dermatitis (AD) and in TNFα/IFNγstimulated HaCaT cells. AD is a chronic inflammatory skin disease and is characterized by erythema, edema, increased pruritus and eczema. Steroids are most commonly used for antiinflammatory therapy; however, their longterm use is limited due to sideeffects, such as osteoporosis, brittle skin, muscle weaknesses and diabetes. Therefore, patients with AD require alternative treatment strategies. In previous studies, SNL has been reported to be effective against oxidants and cancer. However, to the best of our knowledge, the effects of SNL on AD have not yet been investigated. The present study examined the effects of SNL ethanol extract on a model of DNCB induced AD and on TNFα/IFNγstimulated HaCaT cells. The skin tissue was sectioned to measure the thicknesses of the epidermis and dermis, as well as the numbers of eosinophils, mast cells and CD8 infiltration by H&E, toluidine blue, Masson's trichrome and IHC staining. ELISA was performed using serum to measure IgE levels. The present study also examined the expression of various inflammatory cytokines, MAPK and NFκB in TNFα/IFNγstimulated HaCaT cells. SNL significantly reduced the levels of cytokines released from HaCaT cells stimulated with TNFα/IFNγ. SNL also significantly reduced the levels of pp38 at 30 min and significantly reduced the activation of NFκB in a time course experiment. In addition, SNL significantly reduced the level of serum IgE and dermal thickness and the infiltration of mast cells and CD8 in the BALB/c mouse model of DNCBinduced AD. The results of the current study suggest that SNL exerts a suppressive effect on proinflammatory cytokines in vitro and in vivo through the regulation of the immune system.