RESUMO
New sipholane type triterpenes, sipholenols N and O (1 and 2) and neviotine D (3), were isolated from the Red Sea marine sponge Siphonochalina siphonella along with four known triterpenes, sipholenone A (4), sipholenol A (5), siphonellinol D (6) and neviotine A (7). Structure elucidation of 1-3 was achieved by extensive 1D and 2D NMR analyses. The isolated compounds were examined for the inhibition of RANKL induced osteoclastogenesis in RAW264 macrophages. Neviotine D (3) and neviotine A (4) showed potent inhibition with IC50 values of 12.8 and 32.8⯵M, respectively.
Assuntos
Callyspongia/química , Osteogênese/efeitos dos fármacos , Triterpenos/farmacologia , Animais , Oceano Índico , Camundongos , Estrutura Molecular , Ligante RANK , Células RAW 264.7RESUMO
Three new diketopiperazines (1-3), cyclo(l-Pro-d-trans-Hyp) (1), cyclo(l-Pro-d-Glu) (2), and cyclo(d-Pro-d-Glu) (3) and five known diketopiperazines (4-8) were isolated from the endolichenic fungus Colpoma sp. CR1465A identified from the Costa Rican plant Henriettea tuberculosa (Melatomataceae). The structures of the new compounds 1-3 were elucidated using a combination of extensive spectroscopic analyses, including 2D NMR and HR-MS, and their absolute configurations were determined by a combination of NOESY analysis and Marfey's method. Cyclo(l-Pro-d-allo-Thr) (4) was recently isolated from a South China Sea marine sponge Callyspongia sp., but its NMR spectroscopic data were not reported, and cyclo(l-Pro-l-Asp) (5) was previously reported but only as a synthetic product. The NMR data assignments of compounds 4 and 5 are reported for the first time. All of the isolated compounds were tested for antifungal and antimicrobial properties.
Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Callyspongia/química , Dicetopiperazinas/química , Dicetopiperazinas/farmacologia , Peptídeos Cíclicos/química , Peptídeos Cíclicos/farmacologia , Animais , Antibacterianos/química , Antifúngicos/química , Costa Rica , Avaliação Pré-Clínica de Medicamentos/métodos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade MicrobianaRESUMO
Marine sponges are rich sources of natural products exhibiting diverse biological activities. Bioactivity-guided fractionation of the Red Sea sponge Callyspongia aff. implexa led to the isolation of two new compounds, 26,27-bisnorcholest-5,16-dien-23-yn-3ß,7α-diol, gelliusterol E (1) and C27-polyacetylene, callimplexen A (2), in addition to the known compound ß-sitosterol (3). The structures of the isolated compounds were determined by 1D- and 2D-NMR techniques as well as high-resolution tandem mass spectrometry and by comparison to the literature. The three compounds (1-3) were tested against Chlamydia trachomatis, an obligate intracellular gram-negative bacterium, which is the leading cause of ocular and genital infections worldwide. Only gelliusterol E (1) inhibited the formation and growth of chlamydial inclusions in a dose-dependent manner with an IC50 value of 2.3 µM.
Assuntos
Antibacterianos/farmacologia , Produtos Biológicos/farmacologia , Callyspongia/química , Chlamydia trachomatis/efeitos dos fármacos , Poli-Inos/isolamento & purificação , Poli-Inos/farmacologia , Poríferos/química , Esteróis/farmacologia , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Chlamydia trachomatis/crescimento & desenvolvimento , Estrutura Molecular , Poli-Inos/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Sitosteroides/farmacologia , Esteróis/química , Esteróis/isolamento & purificaçãoRESUMO
Bioassay-guided fractionation of the anti-inflammation fractions of the Red Sea sponges Scalarispongia aqabaensis and Callyspongia siphonella yielded two new sterols from chloroform fractions of methanol extracts, namely scalaristerol (5alpha,8alpha-dihydroxycholest-6-en-3beta-ol) (1) from Scalarispongia aqabaensis, and callysterol (ergosta-5,11-dien-3beta-ol) (2) from Callyspongia siphonella. Structure determination was based on extensive NMR studies and mass spectrometry. The antiinflammatory activity of compounds 1 and 2 was assessed using the rat-hind paw edema method and by study of their effect on the release of O2(-) and TXB2 from LPS-activated rat neonatal microglia.
Assuntos
Anti-Inflamatórios/análise , Callyspongia/química , Fitosteróis/isolamento & purificação , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Edema/tratamento farmacológico , Microglia/efeitos dos fármacos , Microglia/metabolismo , Estrutura Molecular , Ratos , Superóxidos/metabolismo , Tromboxano B2/metabolismoRESUMO
A screening of 30 crude extracts of marine sponges against human promyelocytic leukemia cells (HL-60) yielded an EtOAc extract of the sponge Callyspongia sp. (Callyspongiidae) with significant activity. Further bioassay-guided fractionation of the EtOAc extract led to the isolation of three polyacetylene metabolites: a new polyacetylene diol, callyspongidiol (1), along with two known compounds, siphonodiol (2) and 14,15-dihydrosiphonodiol (3). Their structures were determined by a combination of spectroscopic analyses. Compounds 1-3 exhibited antiproliferative activity against HL-60 with IC(50) values of 6.5, 2.8, and 6.5 microg/ml, respectively. These metabolites induce apoptosis in HL-60 cells. Dendritic cells (DC) differentiated with 1-3 enhance the differentiation of naïve T cells towards the Th1 type.