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1.
Nanotechnology ; 33(20)2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35100566

RESUMO

Short time treatment with reduced dosages of selol-loaded PLGA nanocapsules (NcSel) combined with magnetic hyperthermia (MHT) is evaluated in aged Erhlich tumor-bearing mice. Clinical, hematological, biochemical, genotoxic and histopathological parameters are assessed during 7 d treatment with NcSel and MHT, separately or combined. The time evolution of the tumor volume is successfully modeled using the logistic mathematical model. The combined therapy comprising NcSel and MHT is able to hinder primary tumor growth and a case of complete tumor remission is recorded. Moreover, no metastasis was diagnosed and the adverse effects are negligible. NcSel plus MHT may represent an effective and safe alternative to cancer control in aged patients. Future clinical trials are encouraged.


Assuntos
Neoplasias da Mama/terapia , Hipertermia Induzida , Nanopartículas de Magnetita/uso terapêutico , Nanocápsulas/uso terapêutico , Compostos de Selênio/uso terapêutico , Animais , Neoplasias da Mama/patologia , Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Ciclo Celular/efeitos dos fármacos , Terapia Combinada , Fragmentação do DNA/efeitos dos fármacos , Feminino , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/ultraestrutura , Camundongos , Nanocápsulas/química , Nanocápsulas/ultraestrutura , Compostos de Selênio/química , Fatores de Tempo , Resultado do Tratamento , Carga Tumoral/efeitos dos fármacos
2.
Life Sci ; 257: 118108, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32682920

RESUMO

AIM: Preparation of pegylated gold nanorods (PEG-AuNRs) that are capable of converting near infrared (NIR) light into heat. Evaluation of cancer therapeutic efficacy and long-term toxicity of the proposed photothermal therapy in comparison with other conventional modalities. MATERIALS AND METHODS: Prepared PEG-AuNRs were characterized by measuring their absorption spectra, zeta potential, and transmission electron microscope (TEM). Cancer therapeutic efficacy was assessed by monitoring tumor growth, measuring DNA damage and superoxide dismutase (SOD) and malondialdehyde (MDA) levels in addition to examining tumor histopathology. Further analysis concerning the toxicity of all the proposed treatment modalities was also assessed by evaluating the cytotoxicity and genotoxicity in liver and kidney tissues. KEY FINDINGS: The results demonstrated that both photothermal therapy (PEG-AuNRs + NIR laser) and chemotherapy (cisplatin) have higher efficacy in diminishing Ehrlich tumor growth with significance DNA damage over the other treatment modalities. Concerning the biosafety issue, mice treated photothermally exhibited lower MDA level and higher SOD activity in liver and kidney tissues compared with other treated groups. DNA damage represented by tail moment and olive moment of kidney tissues exhibited lower values for photothermal treated group and higher values for cisplatin treated group. SIGNIFICANCE: Photothermal therapy (PEG-AuNRs + NIR laser) potentiates higher efficacy in treating Ehrlich tumor with minimum toxicity in comparison with other conventional treatment modalities.


Assuntos
Carcinoma de Ehrlich/terapia , Ouro/administração & dosagem , Nanotubos/toxicidade , Fototerapia/métodos , Animais , Carcinoma de Ehrlich/patologia , Ensaio Cometa , Dano ao DNA/efeitos dos fármacos , Feminino , Ouro/uso terapêutico , Ouro/toxicidade , Malondialdeído/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica de Transmissão , Nanotubos/ultraestrutura , Transplante de Neoplasias , Estresse Oxidativo , Superóxido Dismutase/metabolismo
3.
Naunyn Schmiedebergs Arch Pharmacol ; 393(8): 1475-1485, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32200461

RESUMO

The present work was to examine a combination of therapy for a low dose of cisplatin and a magnetic field (MF) on Ehrlich carcinoma-bearing mice. In this study, a total of 50 BALB/C female mice were equally distributed into five groups. Mice from the control group did not receive MF or cisplatin. The low and high dose cisplatin groups were injected intraperitoneal (i.p.) with 3 and 6 mg/kg cisplatin, respectively, on the experimental days (1, 4, and 8). Mice group of cisplatin + MF was injected with a low dose of cisplatin followed by MF exposure (50 Hz, 50 mT), and the MF group was exposed to MF only. The impact of MF and cisplatin on the tumor and kidney were evaluated by measuring superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) levels, DNA injury (comet assay), histopathological investigation of tissues, and tumor progress. The results suggested that the combination of a low dose of cisplatin with MF was significantly elevated in MDA levels, reduced SOD activity, and GSH levels. Furthermore, it caused a rise in comet parameters and inhibition in tumor growth. These results showed that MF enhances the therapeutic efficacy of low cisplatin doses and reduces nephrotoxicity.


Assuntos
Antineoplásicos/farmacologia , Carcinoma de Ehrlich/terapia , Cisplatino/farmacologia , Nefropatias/prevenção & controle , Magnetoterapia , Animais , Antineoplásicos/toxicidade , Carcinoma de Ehrlich/metabolismo , Carcinoma de Ehrlich/patologia , Cisplatino/toxicidade , Terapia Combinada , Dano ao DNA , Feminino , Glutationa/metabolismo , Nefropatias/induzido quimicamente , Malondialdeído/metabolismo , Camundongos Endogâmicos BALB C , Estresse Oxidativo/efeitos dos fármacos , Superóxido Dismutase/metabolismo
4.
Nutr Cancer ; 71(8): 1325-1334, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30990087

RESUMO

The topical problem is to find new, more effective and safe treatments for cancer. The purpose of the present work was to study the combined effects of low-intensity extremely high-frequency electromagnetic radiation (EHF EMR) and consumption of n-3 polyunsaturated fatty acids (PUFAs) on tumor growth and the content of FAs in the thymus and tumor tissue in mice. Fatty acid composition was determined using gas chromatography. Exposure of tumor-bearing mice with solid Ehrlich carcinoma to EHF EMR with effective parameters (42.2 GHz, 0.1 mW/cm2, 20 min daily for 5 consecutive days beginning on the first day after the tumor inoculation) led to delaying the tumor growth and restored the content of almost all FAs in thymic tissue to the level of intact animals. Animal intake of the preparation enriched with n-3 PUFAs increased the content of n-3 PUFAs in thymic tissue significantly, but did not affect the tumor growth, even in combination with EHF EMR exposure. Combined action of EHF EMR exposure and n-3 preparation promoted recovery of thymus weight in tumor-bearing animals. The data obtained assume a complex interaction between the immune system and the tumor, and the important role of FAs in the regulation of this interaction.


Assuntos
Carcinoma de Ehrlich/terapia , Radiação Eletromagnética , Ácidos Graxos Ômega-3/metabolismo , Timo/metabolismo , Animais , Carcinoma de Ehrlich/metabolismo , Carcinoma de Ehrlich/patologia , Ácidos Graxos Ômega-3/administração & dosagem , Ácidos Graxos Ômega-3/análise , Sistema Imunitário/efeitos da radiação , Masculino , Camundongos , Timo/efeitos da radiação
5.
Gen Physiol Biophys ; 36(3): 259-271, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28471345

RESUMO

Referable to the limited response of the current available cancer treatment modalities, new effective cancer fighting treatments are needed. This work investigates the efficiency of intratumoural injection of Pseudomonas aeruginosa bacteria combined with a local tumour exposure to extremely low frequency square pulsed magnetic field (ELF SPMF) in the mouse Ehrlich tumour. 64 Ehrlich ascites tumour-implanted female albino BALB/C mice were equally split up into 4 groups. Group 1 (GP1) was the positive control group. Group 2 (GP2) received a single intratumoural injection of P. aeruginosa bacteria. Group 3 (GP3) was exposed to ELF SPMF for tumour local exposure. Group 4 (GP4) was treated with P. aeruginosa intratumoural injection followed by local exposure of the tumour to ELF SPMF. Treatment monitoring was evaluated using ultrastructural examination, flow cytometry analysis in addition to the measurement of tumour dielectric properties. Tumour cell apoptosis was obvious in GP2 and GP4, but, with higher severity and percentages in GP2. Tumour biophysical properties revealed a significant increase in static conductivity σS of GP2, and decreases in dielectric increment Δɛ´of both GP2 and GP4 compared to the GP1. Unfortunately, GP2 mice showed severe signs of toxicity. We advocate the utilization of the combination of P. aeruginosa and SPMF to yield the most effective antitumour agent with less bacteria-related toxicity.


Assuntos
Apoptose/efeitos da radiação , Produtos Biológicos/uso terapêutico , Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Magnetoterapia/métodos , Pseudomonas aeruginosa , Animais , Bacteriorodopsinas , Carcinoma de Ehrlich/microbiologia , Linhagem Celular Tumoral , Terapia Combinada/métodos , Feminino , Injeções Intralesionais , Campos Magnéticos , Camundongos , Camundongos Endogâmicos BALB C , Resultado do Tratamento
6.
Nucleic Acid Ther ; 27(2): 105-114, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-27923103

RESUMO

Magnetomechanical cell disruption using nano- and microsized structures is a promising biomedical technology used for noninvasive elimination of diseased cells. It applies alternating magnetic field (AMF) for ferromagnetic microdisks making them oscillate and causing cell membrane disruption with cell death followed by apoptosis. In this study, we functionalized the magnetic microdisks with cell-binding DNA aptamers and guided the microdisks to recognize cancerous cells in a mouse tumor in vivo. Only 10 min of the treatment with a 100 Hz AMF was enough to eliminate cancer cells from a malignant tumor. Our results demonstrate a good perspective of using aptamer-modified magnetic microdisks for noninvasive microsurgery for tumors.


Assuntos
Aptâmeros de Nucleotídeos/metabolismo , Carcinoma de Ehrlich/terapia , Magnetoterapia/métodos , Campos Magnéticos , Microcirurgia/métodos , Animais , Aptâmeros de Nucleotídeos/síntese química , Carcinoma de Ehrlich/metabolismo , Carcinoma de Ehrlich/patologia , Fibronectinas/metabolismo , Filaminas/metabolismo , Injeções Intralesionais , Magnetoterapia/instrumentação , Imãs , Masculino , Camundongos , Camundongos Endogâmicos ICR , Transplante de Neoplasias , Ligação Proteica , Compostos de Sulfidrila/química
7.
Gen Physiol Biophys ; 35(3): 311-22, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27174899

RESUMO

Nanoscales thermosensitive liposomes (TSL) composed of synthetic lipids (dipalmitoylphosphatidylcholine, and distearoylphosphatidylcholine), were used for doxorubicin encapsulation with 70% encapsulated efficiency. The liposomes were characterized by dynamic light scattering, transmission electron microscopy and turbidity method. Additionally, the liposomes exhibited a significant release of doxorubicin (Dox) by 60% within 5 min at 42°C. To assess the therapeutic efficacy of Dox in combination with hyperthermia, Dox free and encapsulated TSL were administered directly to Ehrlich tumor bearing mice at 1 mg/kg dose. Immediately after the drug administration, hyperthermia was applied to mention the temperature inside the tumor site at 42°C either for 5 min and 30 min. The results indicate a significant increase in the percent of apoptotic and necrotic cells in the treated group. Moreover, disrupts the integrity and the amount of intact DNA in tumor cells. In conclusion, Dox and hyperthermia may serve as a useful targeted drug delivery system for management of Ehrlich carcinoma.


Assuntos
Carcinoma de Ehrlich/terapia , Dano ao DNA , Preparações de Ação Retardada/administração & dosagem , Doxorrubicina/administração & dosagem , Hipertermia Induzida/métodos , Lipossomos/química , Animais , Antibióticos Antineoplásicos/administração & dosagem , Antibióticos Antineoplásicos/química , Carcinoma de Ehrlich/genética , Carcinoma de Ehrlich/patologia , Linhagem Celular Tumoral , Terapia Combinada/métodos , Preparações de Ação Retardada/química , Difusão , Doxorrubicina/química , Temperatura Alta , Camundongos , Nanocápsulas/administração & dosagem , Nanocápsulas/química , Nanocápsulas/ultraestrutura , Tamanho da Partícula , Resultado do Tratamento
8.
Exp Oncol ; 37(2): 94-9, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26112934

RESUMO

AIM: In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells. MATERIALS AND METHODS: EAT cells were exposed to various AsA (0-10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry. RESULTS: Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10-24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6-24 h after treatment. CONCLUSION: These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H2O2 generation at lower AsA concentrations; this carcinostatic effect cannot be exerted by AsA alone.


Assuntos
Antineoplásicos/farmacologia , Ácido Ascórbico/farmacologia , Carcinoma de Ehrlich/terapia , Animais , Apoptose , Pontos de Checagem do Ciclo Celular , Linhagem Celular Tumoral , Proliferação de Células , Sobrevivência Celular , Terapia Combinada , Ensaios de Seleção de Medicamentos Antitumorais , Hipertermia Induzida , Espécies Reativas de Oxigênio/metabolismo
9.
Arq. bras. med. vet. zootec ; Arq. bras. med. vet. zootec. (Online);67(3): 679-688, May-Jun/2015. tab
Artigo em Inglês | LILACS | ID: lil-753939

RESUMO

The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage of CD4+ and CD8+ lymphocytes in Ehrlich tumor-bearing mice. The treatment with the ABM extract in water bath and ultrasound at pH 4 resulted in lower lymphocyte counts, regardless of tumor presence, and greater granulocyte values in mice with Ehrlich tumor than in controls. We concluded that different fractions and methods of extraction of A. blazei produced differing blood profiles in mice inoculated with the Ehrlich tumor.


O objetivo deste estudo foi investigar o efeito de diferentes extratos do cogumelo Agaricus blazeii Murril (ABM) sobre o perfil hematológico de camundongos Swiss portadores de tumor de Ehrlich sólido. Três frações (extrato total, polissacarídeos e sobrenadante) dos extratos de ABM foram obtidas por quatro métodos (sonificador, banho-maria, em pH 4 ou pH 7) e administradas para camundongos durante 21 dias. Soluções de polissacarídeos foram analisadas por cromatografia gasosa e líquida, que mostraram concentrações de glucose e manose. O método de extração influenciou o grau de polimerização da glicose e a relação manose/glucose. O tratamento com o sobrenadante de ABM (em pH 7 e banho-maria) estava associado com reduzidas concentrações de leucócitos e linfócitos, além de alterar a porcentagem de linfócitos CD4+ e CD8+ em camundongos portadores de tumor sólido de Ehrlich. O tratamento com extratos de ABM, obtidos tanto em banho-maria como no sonificador em pH 4, resultou nas mais baixas contagens de linfócitos, independentemente da presença do tumor, e nos maiores valores de granulócitos em camundongos com tumor de Ehrlich. Conclui-se que os diferentes métodos de extração com as respectivas frações de A. blazei são capazes de intereferir no perfil hematológico de camundongos com tumor sólido de Ehrlich.


Assuntos
Animais , Feminino , Camundongos , Agaricus , Carcinoma de Ehrlich/terapia , Extratos Vegetais/uso terapêutico , Glucose/química , Manose/química , Métodos Analíticos de Preparação de Amostras/veterinária , Polimerização , Polissacarídeos/administração & dosagem , Testes Sorológicos/veterinária
10.
Tumour Biol ; 35(4): 3391-403, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24292954

RESUMO

Dextran-functionalized maghemite fluid (DexMF) has been tested to treat Ehrlich-solid-tumor-bearing mice, evidencing its potential use in mediating magnetohyperthermia in breast cancer treatment. However, although magnetic nanoparticles tend to accumulate in tumor tissues, part of the nanomaterial can reach the blood stream, and then the organism. The aim of this study was to investigate the acute systemic effects of the intratumoral injection of DexMF mediating magnetohyperthermia in the treatment of an advanced clinical Ehrlich-solid-tumor, assessed through histopathological analyses of liver, kidneys, heart and spleen, comet assay, micronucleus test, hemogram, and serum levels of bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, creatinine, and urea. The tumor's histopathology and morphometry were used to assess its aggressiveness and regression. DexMF mediating hyperthermia was effective in containing tumor aggressiveness and in inducing tumor regression, besides showing no toxic effects. Its physical characteristics also suggest that it is safe to use in other biomedical applications.


Assuntos
Carcinoma de Ehrlich/terapia , Dextranos/administração & dosagem , Hipertermia Induzida/métodos , Magnetoterapia/métodos , Animais , Carcinoma de Ehrlich/genética , Carcinoma de Ehrlich/patologia , Ensaio Cometa , Feminino , Hipertermia Induzida/efeitos adversos , Magnetoterapia/efeitos adversos , Imageamento por Ressonância Magnética , Camundongos , Micronúcleos com Defeito Cromossômico , gama-Glutamiltransferase/sangue
11.
J Pharm Sci ; 102(12): 4395-405, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24136132

RESUMO

We investigated antitumor, genotoxic, chemopreventive, and immunostimulative effects of local chemoimmunotherapy and hyperthermal intraperitoneal chemotherapy (HIPEC) in a mouse-bearing Ehrlich ascites tumor (EAT). Mice were treated with water-soluble derivative of propolis (WSDP) at a dose of 50 mg kg(-1) , 7 and 3 days before implantation of EAT cells, whereas cisplatin (5 or 10 mg kg(-1) ) was injected 3 days after implantation of EAT cells at 37°C and 43°C. The following variables were analyzed: the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, comet assay, and micronucleus assay. The combination of WSDP + CIS 5 mg kg(-1) at 37°C resulted in tumor growth inhibition and increased the survival of mice by additional 115.25%. WSDP with HIPEC increased the survival of mice by additional 160.3% as compared with HIPEC. WSDP reduced cisplatin toxic and genotoxic effect to normal cells without affecting cisplatin cytotoxicity on EAT cells. In addition, WSDP with HIPEC increased the cytotoxic actions of macrophages to tumor cells. Water-soluble derivative of propolis increases macrophage activity and sensitivity of tumor cells to HIPEC and reduces cisplatin toxicity to normal cells.


Assuntos
Adjuvantes Imunológicos/uso terapêutico , Antineoplásicos/uso terapêutico , Carcinoma de Ehrlich/terapia , Cisplatino/uso terapêutico , Própole/uso terapêutico , Adjuvantes Imunológicos/administração & dosagem , Adjuvantes Imunológicos/farmacologia , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/farmacologia , Carcinoma de Ehrlich/imunologia , Carcinoma de Ehrlich/patologia , Cisplatino/administração & dosagem , Cisplatino/farmacologia , Sinergismo Farmacológico , Hipertermia Induzida , Imunoterapia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/patologia , Masculino , Camundongos , Própole/administração & dosagem , Própole/farmacologia
12.
PLoS One ; 8(10): e76207, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24098446

RESUMO

Gold nanorods (GNR) within tumor microregions are characterized by their ability to absorb near IR light and emit heat in what is called photoplasmonic effect. Yet, the efficacy of nanoparticles is limited due to intratumoral tissue distribution reasons. In addition, distribution of GNRs to normal tissue might result in non specific toxicity. In the current study, we are assessing the intratumoral and tissue distribution of PEGylated GNRs on the top of its antitumor characteristics when given intravenously or intratumoral to solid tumor bearing mice and coupled with laser photoplasmonic sessions. PEGylated GNRs with a longitudinal size of less than 100 nm were prepared with aspect ratio of 4.6 showing strong surface plasmon absorption at wavelength 800 nm. Pharmacokinetics of GNR after single I.V. administration (0.1 mg/kg) showed very short systemic circulating time (less than 3 h). On the other hand, tissue distribution of I.V. GNR (0.1 mg/kg) to normal animals showed preferential deposition in spleen tissue. Repeated administration of I.V. GNR resulted in preferential accumulation in both liver and spleen tissues. In addition, I.V. administration of GNR to Ehrlich carcinoma tumor bearing mice resulted in similar tissue distribution; tumor accumulation and anti-tumor effect compared to intratumoral administration. In conclusion, the concentration of GNR achieved within tumors microregions after I.V. administration was comparable to I.T. administration and sufficient to elicit tumoral growth arrest when coupled with laser-aided photoplasmonic treatment.


Assuntos
Carcinoma de Ehrlich/metabolismo , Ouro , Nanotubos , Administração Intravenosa , Animais , Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Modelos Animais de Doenças , Feminino , Ouro/química , Hipertermia Induzida , Terapia com Luz de Baixa Intensidade , Masculino , Camundongos , Nanotubos/química , Nanotubos/ultraestrutura , Polietilenoglicóis/química , Distribuição Tecidual , Carga Tumoral
13.
J Biomed Nanotechnol ; 9(7): 1261-71, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23909142

RESUMO

This work aimed to test a dextran-functionalized magnetic fluid (DexMF) sample in mediating magnetohyperthermia to treat an advanced clinical Ehrlich-solid-tumor, to verify the effects of oral antioxidant administration of pequi-oil on this treatment and to investigate the potential of these treatments for future use as an adjuvant in cancer therapy. Animals received the treatments: (a) filtered water (control); (b) tumor implantation and no treatment (tumor group); (c) tumor implantation followed by intratumoral injection of DexMF and alternating current magnetic field exposure (MHT group) for three consecutive days; (d) oral pequi-oil supplementation followed by tumor implantation and the same treatment as group MHT (PMHT group). Analyses took place 1 and 2 weeks after tumor implantation. Both treatments were effective in increasing the tumor necrosis process and controlling tumor growth, besides keeping lymphocyte-dependent immunity. Although the MHT treatment was more efficient after the first week in reducing DNA damage to blood peripheral leucocytes, PMHT therapy appeared to be more effective with the advance of the carcinogenesis process after the second week. Our findings evidence the potential use of DexMF mediating magnetohyperthermia in cancer treatment and also suggest that the preventive pequi oil administration could increase the efficiency of this process.


Assuntos
Carcinoma de Ehrlich/terapia , Dextranos/química , Hipertermia Induzida/métodos , Magnetoterapia/métodos , Óleos de Plantas/uso terapêutico , Animais , Antioxidantes/administração & dosagem , Carcinoma de Ehrlich/patologia , Linhagem Celular Tumoral , Terapia Combinada/métodos , Estudos de Viabilidade , Resultado do Tratamento
14.
Rev. cuba. plantas med ; 17(2)abr.-jun. 2012.
Artigo em Espanhol | CUMED | ID: cum-50972

RESUMO

Introducción: hoy día los científicos recurren con mayor frecuencia a la naturaleza para desarrollar fármacos contra enfermedades como el cáncer. Los productos naturales son diversos en cuanto a su origen y estructuralmente complejos; pueden ser capaces a partir de un efecto indirecto de inhibir el desarrollo del tumor por medio de la estimulación de los mecanismos de defensa del organismo. Objetivo: evaluar la actividad antitumoral indirecta de extractos de plantas en el tumor ascítico de Erlich. Métodos: se seleccionaron 50 ratones de la línea NMRI, a los cuales se les hizo un pretratamiento por vía intraperitoneal con los extractos obtenidos de plantas durante 5 d; a las 48 h después del tratamiento se trasplantaron las células del tumor ascítico de Erlich. A otro grupo de animales utilizados como control se les realizó el pretratamiento con solución salina fisiológica 0,9 por cientoy a las 48 h se efectuó el trasplante con las mismas células tumorales. La actividad antitumoral se determinó comparando el grupo control con el tratado mediante los extractos, por rechazo al implante del tumor. Se consideró que el producto era activo si provocaba un valor igual o mayor que 60 % de inhibición del crecimiento tumoral de los animales tratados. Resultados: de los extractos evaluados, 5 mostraron en una de las dosis ensayadas un valor mayor que 60 por ciento de animales vivos, sin signos de presencia del tumor a los 30 d de observación. Esto demuestra que esos extractos son activos. Conclusiones: los 5 extractos podrían ser probables productos estimuladores de la respuesta inmune, lo que los hace candidatos a continuar las investigaciones(AU)


Introduction: at present, scientists are resorting more frequently to nature in order to develop drugs against various illnesses such as cancer. The origin of natural products is diverse and their structure is very complex with the capacity of inhibiting through indirect effect the development of tumors by stimulating the defense mechanisms of the body. Objective: to evaluate the indirect antitumor action of plant extracts in Erlich ascitic tumor cells. Methods: a group of 50 NMRI-line mice was interperitoneally pretreated with plant extracts during 5 days. After 48 hours, Erlich ascitic tumor cells were transplanted into the mice. Another control group of mice was pretreated with 0,9 percent physiological saline solution; at 48 hours they were also implanted the same tumor cells. The antitumor activity was determined by comparing the control group with the group of mice treated with extracts in terms of tumor implant rejection. The product was considered to be active if it caused 60 percent tumor growth inhibition or more. Results: of the evaluated extracts, 5 obtained more than 60 percent of survival in animals at one of the tested doses, with no signs of tumor after observation during 30 days. It proved that these extracts were active. Conclusions: it was proved that these 5 extracts could be potential products in stimulating immune response, so they should be further studied in the future(AU)


Assuntos
Animais , Camundongos , Ensaios de Seleção de Medicamentos Antitumorais , Extratos Vegetais/uso terapêutico , Carcinoma de Ehrlich/terapia
15.
Rev. cuba. plantas med ; 17(2): 115-124, abr.-jun. 2012.
Artigo em Espanhol | LILACS | ID: lil-629694

RESUMO

Introducción: hoy día los científicos recurren con mayor frecuencia a la naturaleza para desarrollar fármacos contra enfermedades como el cáncer. Los productos naturales son diversos en cuanto a su origen y estructuralmente complejos; pueden ser capaces a partir de un efecto indirecto de inhibir el desarrollo del tumor por medio de la estimulación de los mecanismos de defensa del organismo. Objetivo: evaluar la actividad antitumoral indirecta de extractos de plantas en el tumor ascítico de Erlich. Métodos: se seleccionaron 50 ratones de la línea NMRI, a los cuales se les hizo un pretratamiento por vía intraperitoneal con los extractos obtenidos de plantas durante 5 d; a las 48 h después del tratamiento se trasplantaron las células del tumor ascítico de Erlich. A otro grupo de animales utilizados como control se les realizó el pretratamiento con solución salina fisiológica 0,9 por cientoy a las 48 h se efectuó el trasplante con las mismas células tumorales. La actividad antitumoral se determinó comparando el grupo control con el tratado mediante los extractos, por rechazo al implante del tumor. Se consideró que el producto era activo si provocaba un valor igual o mayor que 60 % de inhibición del crecimiento tumoral de los animales tratados. Resultados: de los extractos evaluados, 5 mostraron en una de las dosis ensayadas un valor mayor que 60 por ciento de animales vivos, sin signos de presencia del tumor a los 30 d de observación. Esto demuestra que esos extractos son activos. Conclusiones: los 5 extractos podrían ser probables productos estimuladores de la respuesta inmune, lo que los hace candidatos a continuar las investigaciones


Introduction: at present, scientists are resorting more frequently to nature in order to develop drugs against various illnesses such as cancer. The origin of natural products is diverse and their structure is very complex with the capacity of inhibiting through indirect effect the development of tumors by stimulating the defense mechanisms of the body. Objective: to evaluate the indirect antitumor action of plant extracts in Erlich ascitic tumor cells. Methods: a group of 50 NMRI-line mice was interperitoneally pretreated with plant extracts during 5 days. After 48 hours, Erlich ascitic tumor cells were transplanted into the mice. Another control group of mice was pretreated with 0,9 percent physiological saline solution; at 48 hours they were also implanted the same tumor cells. The antitumor activity was determined by comparing the control group with the group of mice treated with extracts in terms of tumor implant rejection. The product was considered to be active if it caused 60 percent tumor growth inhibition or more. Results: of the evaluated extracts, 5 obtained more than 60 percent of survival in animals at one of the tested doses, with no signs of tumor after observation during 30 days. It proved that these extracts were active. Conclusions: it was proved that these 5 extracts could be potential products in stimulating immune response, so they should be further studied in the future


Assuntos
Animais , Camundongos , Carcinoma de Ehrlich/terapia , Ensaios de Seleção de Medicamentos Antitumorais , Extratos Vegetais/uso terapêutico
16.
Langmuir ; 28(24): 8994-9002, 2012 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-22404289

RESUMO

We describe a novel strategy for the fabrication of plasmonic nanopowders (dried gold nanoparticles) by using wet chemical nanoparticle synthesis, PEG-SH functionalization, and a standard freeze-drying technique. Our strategy is illustrated by successful fabrication of different plasmonic nanopowders, including gold nanorods, gold-silver nanocages, and gold nanospheres. Importantly, the dried nanoparticles can be stored for a long time under usual conditions and then can easily be dissolved in water at a desired concentration without such hard manipulations as sonication or heating. Redispersed samples maintain the plasmonic properties of parent colloids and do not form aggregates. These properties make pegylated freeze-dried gold nanoparticles attractive candidates for plasmonic photothermal therapy in clinical settings. In this work, redispersed gold nanorods were intravenously administered to mice bearing Ehrlich carcinoma tumors at doses of 2 and 8 mg (Au)/kg (animal). Particle biodistribution was measured by atomic absorption spectroscopy, and tumor hyperthermia effects were studied under laser NIR irradiation. Significant tumor damage was observed only at the higher dose of the nanorods.


Assuntos
Carcinoma de Ehrlich/terapia , Ouro/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Ressonância de Plasmônio de Superfície , Animais , Carcinoma de Ehrlich/patologia , Feminino , Ouro/química , Lasers , Nanopartículas Metálicas/química , Camundongos , Camundongos Endogâmicos BALB C , Fototerapia , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Magn Reson Imaging ; 29(2): 272-80, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21145190

RESUMO

Super paramagnetic iron oxide Fe(3)O(4) nanoparticles prepared via photochemical reaction in pure form were used for inducing hyperthermia to treat subcutaneous Ehrlich carcinoma implanted in female mice. Our results indicate that the mean temperature profiles at the rectum, periphery of the tumor surface and at the center of the tumor during hyperthermia treatment increased gradually. The maximum temperature achieved in the tumor center was 47±1°C after 20 min with radiofrequency exposures at 25 kW. The acquired magnetic resonance images identified apoptotic cells in the center of the tumor which were exposed to magnetic resonance hyperthermia (MRH). Apoptotic cells presented as dark signal intensity in the T(1)-weighted images which were further confirmed by pathological examinations. Also, the results revealed that the tumor size in the all mice exposed to MRH is still as the same as before the treatment, but the rate of tumor growth was very slow by comparing with the growth rate of the control group.


Assuntos
Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Hipertermia Induzida/métodos , Imageamento por Ressonância Magnética/métodos , Nanopartículas Metálicas/uso terapêutico , Animais , Linhagem Celular Tumoral , Feminino , Magnetismo/métodos , Camundongos , Resultado do Tratamento
18.
Exp Oncol ; 32(1): 48-50, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20332757

RESUMO

UNLABELLED: It is proposed to use the novel paradigm of treating cancer with hyperthermic therapy using multi-walled carbon nanotubes (MWCNT) stimulated with near infrared (NIR) irradiation. AIM: To establish the capacity of MWCNT stimulated with NIR irradiation to destroy Erlich ascitic carcinoma (EAC) cells. MATERIALS AND METHODS: EAC cells suspension was irradiated with NIR heating lamp with a wavelength of 0.78-1.40 mm and power density of 3.5 W/cm2 over 1.5 min in the presence of MWCNT (0.1 mg/ml). The changes in the temperature of suspension with the NIR exposure time was measured using the differential cooper-constantan thermocouple. The viability of EAC cells was evaluated by trypan blue staining. RESULTS: The death of 95.2% of EAC cells in the presence of MWCNT was observed after 1.5 min of NIR light irradiation: thermal ablation temperature was approximately 50 degrees C. CONCLUSIONS: It was demonstrated that addition of MWCNT to EAC cell suspension results in the photo-ablative destruction of cells exposed to short time NIR irradiation.


Assuntos
Hipertermia Induzida/métodos , Raios Infravermelhos/uso terapêutico , Nanotubos de Carbono/efeitos da radiação , Neoplasias/terapia , Animais , Animais não Endogâmicos , Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Morte Celular , Camundongos , Nanotubos de Carbono/química , Transplante de Neoplasias , Neoplasias/patologia , Temperatura , Fatores de Tempo , Células Tumorais Cultivadas
19.
J Biomed Nanotechnol ; 6(6): 687-93, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21361134

RESUMO

The present study examines the feasibility of a low power argon laser-induced thermal therapy to Ehrlich carcinoma, employing a direct administration of spherical gold nanoparticles (GNPs). This modality utilizes the advantage of strong surface plasmon resonance exhibited by spherical GNPs in the visible range. Ehrlich tumors were grown in female balb mice by subcutaneous injection of Ehrlich ascites carcinoma cells. GNPs with an average diameter 13 +/- 1.2 nm and optical density (ODlambda:518 nm = 3) were directly injected within the tumor interstitium. Tumors were then illuminated with a continuous-wave (CW) argon ion laser with irradiance 55 mW cm-2 for 45 min. All laser-GNPs treated tumors exhibited a significant suppression in tumor growth throughout 15 days. On the contrary, sham-treated group (laser treatment without GNPs injection) and control group (neither laser nor GNPs treatment) showed a progressive increase in tumor growth during the same period. Histopathological examination demonstrated extensive necrotic percentage in laser-GNPs treated group (90%) in comparison with sham (35%) or control group (3-7%). A wide-angle X-ray scattering also revealed detectable changes in tumor protein structure exposed to both laser and GNPs. It can be concluded from this study that the intense surface plasmon resonance exhibited by spherical GNPs in the visible range could be very useful as a noninvasive technique for photothermal therapy of skin or near-surface type tumors that need much less laser energy and lower concentrations of GNPs.


Assuntos
Carcinoma de Ehrlich/terapia , Ouro/uso terapêutico , Lasers de Gás/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Animais , Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/radioterapia , Feminino , Ouro/farmacologia , Terapia com Luz de Baixa Intensidade , Camundongos , Camundongos Endogâmicos BALB C , Tela Subcutânea/efeitos dos fármacos , Difração de Raios X
20.
Bioelectromagnetics ; 30(5): 343-51, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19267367

RESUMO

It has been shown that the ultralow-frequency extremely weak alternating component of combined magnetic fields (MFs) exhibits a marked antitumor activity. The parameters of this component have been found (frequency 1, 4.4, 16.5 Hz or the sum of these frequencies; intensity 300, 100, 150-300 nT, respectively) at which this MF in combination with a collinear static MF of 42 microT inhibits or suppresses the growth of Ehrlich ascites carcinoma (EAC) in mice. It was shown that the exposure of mice with EAC to combined MFs causes structural changes in some organs (liver, adrenal glands), which are probably due to the total degradation of the tumor tissue. In mice with transplanted EAC, the tumor tissue after exposure to weak MFs was practically absent, as distinct from control animals in which the invasion of the tumor into the adipose tissue surrounding the kidneys, mesenteric lymph nodes, and spermatic appendages was observed. In animals without tumors, no pathological deviations from the norm in the structure of organs and tissues occurred after exposure to weak MF, indicating that this factor per se is not toxic to the organism.


Assuntos
Carcinoma de Ehrlich/patologia , Carcinoma de Ehrlich/terapia , Magnetoterapia/métodos , Magnetismo , Animais , Magnetoterapia/efeitos adversos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Fatores de Tempo
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