RESUMO
The identification of aroma composition and key odorants contributing to aroma characteristics of white tea is urgently needed, owing to white tea's charming flavors and significant health benefits. In this study, a total of 238 volatile components were identified in the three subtypes of white teas using headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS). The multivariate statistical analysis demonstrated that the contents of 103 volatile compounds showed extremely significant differences, of which 44 compounds presented higher contents in Baihaoyinzhen and Baimudan, while the other 59 compounds exhibited higher contents in Shoumei. The sensory evaluation experiment carried out by gas chromatography-olfactometry/mass spectrometry (GC-O/MS) revealed 44 aroma-active compounds, of which 25 compounds were identified, including 9 alcohols, 6 aldehydes, 5 ketones, and 5 other compounds. These odorants mostly presented green, fresh, floral, fruity, or sweet odors. Multivariate analyses of chemical characterization and sensory evaluation results showed that high proportions of alcohols and aldehydes form the basis of green and fresh aroma characteristic of white teas, and phenylethyl alcohol, γ-Nonalactone, trans-ß-ionone, trans-linalool oxide (furanoid), α-ionone, and cis-3-hexenyl butyrate were considered as the key odorants accounting for the different aroma characteristics of the three subtypes of white tea. The results will contribute to in-depth understand chemical and sensory markers associated with different subtypes of white tea, and provide a solid foundation for tea aroma quality control and improvement.
Assuntos
Aromatizantes/química , Odorantes/análise , Chá/química , Monoterpenos Acíclicos/química , Aldeídos/química , Cicloexanóis/química , Frutas/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Norisoprenoides/química , Microextração em Fase Sólida/métodos , Compostos de Tritil/química , Compostos Orgânicos Voláteis/químicaRESUMO
The unpleasant stale note is a negative factor hindering the consumption of instant ripened Pu-erh tea products. This study focused on investigating volatile chemicals in instant ripened Pu-erh tea that could mask the stale note via sensory evaluation, gas chromatography-mass spectrometry (GC-MS), and gas chromatography-olfactometry (GC-O) analyses. GC-MS and GC-O analyses showed that linalool, linalool oxides, trans-ß-ionone, benzeneacetaldehyde, and methoxybenzenes were the major aroma contributors to the simultaneous distillation and extraction (SDE) extract of instant ripened Pu-erh tea. Sensory evaluation showed that the SDE extract had a strong stale note, which was due to methoxybenzenes. By investigating suppressive interaction among flavour components, the stale note from methoxybenzenes was shown to have reciprocal masking interactions with sweet, floral, and green notes. Moreover, the validation experiment showed that the addition of 40 µg/mL of trans-ß-ionone in the instant ripened Pu-erh tea completely masked the stale note and improved the overall aromatic acceptance. These results elucidate the volatile chemicals that could mask the stale note of instant ripened Pu-erh tea products, which might help to develop high quality products made from instant ripened Pu-erh tea.
Assuntos
Extratos Vegetais/química , Chá/química , Monoterpenos Acíclicos/química , Anisóis/química , Cicloexanóis/química , Cromatografia Gasosa-Espectrometria de Massas , Compostos de Tritil/químicaRESUMO
Bacterial periplasmic-binding proteins have been acclaimed as general biosensing platform, but their range of natural ligands is too limited for optimal development of chemical compound detection. Computational redesign of the ligand-binding pocket of periplasmic-binding proteins may yield variants with new properties, but, despite earlier claims, genuine changes of specificity to non-natural ligands have so far not been achieved. In order to better understand the reasons of such limited success, we revisited here the Escherichia coli RbsB ribose-binding protein, aiming to achieve perceptible transition from ribose to structurally related chemical ligands 1,3-cyclohexanediol and cyclohexanol. Combinations of mutations were computationally predicted for nine residues in the RbsB binding pocket, then synthesized and tested in an E. coli reporter chassis. Two million variants were screened in a microcolony-in-bead fluorescence-assisted sorting procedure, which yielded six mutants no longer responsive to ribose but with 1.2-1.5 times induction in presence of 1 mM 1,3-cyclohexanediol, one of which responded to cyclohexanol as well. Isothermal microcalorimetry confirmed 1,3-cyclohexanediol binding, although only two mutant proteins were sufficiently stable upon purification. Circular dichroism spectroscopy indicated discernable structural differences between these two mutant proteins and wild-type RbsB. This and further quantification of periplasmic-space abundance suggested most mutants to be prone to misfolding and/or with defects in translocation compared to wild-type. Our results thus affirm that computational design and library screening can yield RbsB mutants with recognition of non-natural but structurally similar ligands. The inherent arisal of protein instability or misfolding concomitant with designed altered ligand-binding pockets should be overcome by new experimental strategies or by improved future protein design algorithms.
Assuntos
Sítios de Ligação , Cicloexanóis/química , Desenho de Fármacos , Proteínas de Escherichia coli/química , Escherichia coli/metabolismo , Modelos Moleculares , Proteínas Periplásmicas de Ligação/química , Aminoácidos , Avaliação Pré-Clínica de Medicamentos , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Proteínas de Escherichia coli/antagonistas & inibidores , Proteínas de Escherichia coli/genética , Biblioteca Gênica , Ligantes , Mutação , Proteínas Periplásmicas de Ligação/antagonistas & inibidores , Proteínas Periplásmicas de Ligação/genética , Relação Estrutura-AtividadeRESUMO
Antibacterial resistance is an increasingly serious threat to global public health. The search for new anti-infection agents from natural resources, with different mode of actions and competitive effects became a necessity. In this study, twenty height methicillin-resistant Staphylococcus aureus (MRSA) strains were investigated for their biofilm formation ability. Subsequently, the antibiofilm effects of Eucalyptus globulus essential oil and its main component 1,8-cineole, against MRSA, as well as their antiquorum sensing potential, were evaluated. Our results displayed the potent efficacy of both E. globulus essential oil and 1,8-cineole against the development of biofilms formed by the methicillin-resistant strains. Additionally, E. globulus essential oil showed more potent of anti-QS activity, even at a low concentration, when compared to 1,8-cineole. All these property of tested agents may pave the way to prevent the development of biofilm formation by MRSA and subsequently the spreading of nosocomial infection.
Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Cicloexanóis/farmacologia , Eucalyptus/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Monoterpenos/farmacologia , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Percepção de Quorum/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Cicloexanóis/química , Eucaliptol , Testes de Sensibilidade Microbiana , Monoterpenos/química , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Óleos de Plantas/isolamento & purificação , Óleos de Plantas/farmacologiaRESUMO
Epidermal ß-glucocerebrosidase (GBA1), an acid ß-glucosidase normally located in lysosomes, converts (glucosyl)ceramides into ceramides, which is crucial to generate an optimal barrier function of the outermost skin layer, the stratum corneum (SC). Here we report on two developed in situ methods to localize active GBA in human epidermis: i) an optimized zymography method that is less labor intensive and visualizes enzymatic activity with higher resolution than currently reported methods using either substrate 4-methylumbelliferyl-ß-D-glucopyranoside or resorufin-ß-D-glucopyranoside; and ii) a novel technique to visualize active GBA1 molecules by their specific labeling with a fluorescent activity-based probe (ABP), MDW941. The latter method pro-ved to be more robust and sensitive, provided higher resolution microscopic images, and was less prone to sample preparation effects. Moreover, in contrast to the zymography substrates that react with various ß-glucosidases, MDW941 specifically labeled GBA1. We demonstrate that active GBA1 in the epidermis is primarily located in the extracellular lipid matrix at the interface of the viable epidermis and the lower layers of the SC. With ABP-labeling, we observed reduced GBA1 activity in 3D-cultured skin models when supplemented with the reversible inhibitor, isofagomine, irrespective of GBA expression. This inhibition affected the SC ceramide composition: MS analysis revealed an inhibitor-dependent increase in the glucosylceramide:ceramide ratio.
Assuntos
Ensaios Enzimáticos , Corantes Fluorescentes/química , Glucosilceramidase/análise , Pele/enzimologia , Coloração e Rotulagem/métodos , Benzoxazinas/química , Compostos de Boro/química , Cicloexanóis/química , Compostos de Epóxi/química , Expressão Gênica , Glucosídeos/química , Glucosilceramidase/metabolismo , Humanos , Himecromona/análogos & derivados , Himecromona/química , Técnicas de Cultura de TecidosRESUMO
Monogenean parasites are important ectoparasites of fish, and are responsible for severe economic impacts in the aquaculture industry. They are usually treated with chemicals, but the chemicals can have harmful side effects in the fish and may pose threats to human health. Rosemary (Rosmarinus officinalis) is a common medicinal herb, with antimicrobial and antitumor properties. Here, we examined the anthelmintic activity of rosemary extract against the monogenean (Dactylogyrus minutus) in vitro and in vivo using bath treatment and oral administration. The in vitro experiments showed that parasite survival was affected by both rosemary extract concentration and the solvent (water and ethanol). Parasites were dead at 61.8±5.6 and 7.8±1.4min when exposed to 100 and 200g aqueous rosemary extract solution/L of water respectively. It took 166.7±48.2 and 5.4±1.01min to kill the parasites when exposed to 1 and 32g ethanol rosemary extract solution/L of water respectively. Moreover, pure component of rosemary extract obtained commercially used in in vitro experiments showed that 1,8-Cineole was the most toxic component of the main components tested. Parasite intensity and prevalence in fish exposed to 50 and 100g aqueous rosemary solution/L water for 30min were significantly lower than they were in controls (p<0.05). In oral treatment experiments, diets of Cyprinus carpio were supplemented with eight different concentrations of aqueous rosemary extract. The intensity of parasites was significantly less in fish fed for 30days with feed containing 60, 80 and 100ml aqueous extract/100g feed than in control (p<0.05). Together these results indicate that rosemary is a promising candidate for prevention and control of monogenean infection.
Assuntos
Anti-Helmínticos/farmacologia , Carpas/parasitologia , Doenças dos Peixes/tratamento farmacológico , Helmintíase Animal/tratamento farmacológico , Extratos Vegetais/farmacologia , Rosmarinus/química , Animais , Anti-Helmínticos/química , Anti-Helmínticos/isolamento & purificação , Aquicultura , Cicloexanóis/química , Cicloexanóis/isolamento & purificação , Cicloexanóis/farmacologia , Eucaliptol , Doenças dos Peixes/parasitologia , Doenças dos Peixes/prevenção & controle , Helmintíase Animal/parasitologia , Helmintíase Animal/prevenção & controle , Monoterpenos/química , Monoterpenos/isolamento & purificação , Monoterpenos/farmacologia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Plantas Medicinais , Platelmintos/efeitos dos fármacosRESUMO
The aim of the study was the determination of the chemical composition of Mentha pulegium L. and Rosmarinus officinalis L. essential oils and the evaluation of their antileishmanial, antibacterial and antioxidant activities. Essential oils (EOs) were isolated using steam distillation and the chemical composition was determined using GC-MS analysis. The antibacterial activity was tested against ten pathogenic strains using the diffusion method, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) by microtitration assay. The antioxidant activity was estimated by DPPH free radical scavenging ability and ferric-reducing power. The antileishmanial activity was tested against Leishmania major, Leishmania tropica and Leishmania infantum using MTT (3-(4.5-dimethylthiazol-2yl)-2.5-diphenyltetrazolium bromide) assay. The yield of essential oils (v/w %) M. puleguim and R. officinalis based on dry weight were 5.4 and 2.7% respectively. GC/MS analysis of R. officinalis essential oil (ROEO) revealed the presence of 29 components, mainly represented by oxygenated monoterpenes (63.743%) and hydrocarbons monoterpenes (21.231%). Mentha pulegium essential oil (MPEO) revealed 21 components, mainly represented by oxygenated monoterpenes (83.865%). The major components of ROEO were α-pinene (14.076), 1,8-Cineole (23.673) and camphor (18.743), while menthone (21.164) and pulegone (40.98) were the main major components of MPEO. M. pulegium and R. officinalis EOs showed a significant antioxidant activity compared with ascorbic acid and Trolox to the IC50 values of 58.27 ± 2.72 and 85.74 ± 7.57 µg/mL respectively revealed by reducing power assay. As for the antibacterial effect, the highest zone diameters were shown by the MPEO against Bacillus subtilis (30 ± 1.43 mm) and Proteus mirabilis (28 ± 1.32 mm). These values are significantly important compared with those of the commercialized antibiotic (Erythromycin and Chlorophenicol). The lowest MIC and MBC values were obtained with MPEO against S. aureus MBLA (MIC = MBC = 0.25% (v/v)). While, ROEO has exhibited a bactericidal effect against Listeria monocytogenes (MIC = MBC = 0.5% (v/v)), Bacillus subtilis (MIC = MBC = 1% (v/v)) and Escherichia coli (MIC = MBC = 1% (v/v)). For the antileishmanial effect, ROEO is the most active against L. major (IC50 = 1.2 ± 0.36 µg/mL. While, the MPEO has the most leishmanicidal effect against L. major (IC50 = 1.3 ± 0.45 µg/mL). These findings show that the EOs of M. pulegium and R. officinalis are good sources of bioactive molecules that could have potential applications in the food and pharmaceutical industries.
Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Mentha pulegium/química , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Rosmarinus/química , Antioxidantes/química , Bacillus subtilis/efeitos dos fármacos , Monoterpenos Bicíclicos , Cânfora/química , Monoterpenos Cicloexânicos , Cicloexanóis/química , Escherichia coli/efeitos dos fármacos , Eucaliptol , Sequestradores de Radicais Livres , Cromatografia Gasosa-Espectrometria de Massas , Leishmania/classificação , Leishmania/efeitos dos fármacos , Mentol/química , Testes de Sensibilidade Microbiana , Monoterpenos/química , Marrocos , Staphylococcus aureus/efeitos dos fármacosRESUMO
The poultry red mite, Dermanyssus gallinae, represents a key threat for the poultry industry worldwide. The control of D. gallinae is mainly achieved by continuous applications of acaricides. However, the fast-growing development of resistance, and the strict laws concerning chemicals admitted for treatments on food animals, highlighted the importance of alternative control tools. Here, we explored the potential of Artemisia sieberi essential oil against D. gallinae. In this study, the A. sieberi essential oil was analyzed using GC and GC-MS. The oil toxicity through contact and fumigant assays on adult mites was evaluated. The oil repellent activity was assessed on adult mites over different time intervals. Lastly, the residual toxicity of various doses of the oil was evaluated on D. gallinae until 14 days post treatment. GC and GC-MS showed that the oil was rich in α-thujone (31.5%), ß-thujone (11.92%), camphor (12.3%), and 1,8-cineole (10.09%). Contact toxicity on adult mites showed 50% lethal concentration (LC50), LC90, and LC99 of 15.85, 26.63, and 35.42 µg/cm3, respectively. In fumigant assays, the oil was toxic on D. gallinae, and mortality was significantly higher in open containers over closed ones, underlining the key role of highly volatile constituents. Repellent assays showed that after 24 h from the treatment, all doses of the A. sieberi essential oil led to significant repellent activity over the control, except for 2 µg/cm3. After 48 h, A. sieberi essential oil tested at all doses led to significant repellent activity, if compared to the control. Residual toxicity assays showed that time exposure and concentration tested had a significant impact on mite mortality after 1, 2, 5, and 7 days from the treatment. Notably, mortality remained significantly higher over the control for 7 days after spraying with oil at 2%. Further field assays with selected molecules from the A. sieberi essential oil are ongoing, testing them in synergistic blends, as well as in microencapsulated formulations.
Assuntos
Acaricidas/farmacologia , Artemisia/química , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Óleos Voláteis/análise , Óleos Voláteis/farmacologia , Trombiculidae/crescimento & desenvolvimento , Animais , Monoterpenos Bicíclicos , Cânfora/química , Cicloexanóis/química , Resistência a Medicamentos , Eucaliptol , Cromatografia Gasosa-Espectrometria de Massas , Repelentes de Insetos/química , Inseticidas/química , Monoterpenos/química , Óleos Voláteis/química , Aves Domésticas/parasitologia , Trombiculidae/efeitos dos fármacosRESUMO
The phytochemical profile of decoction and infusion, obtained from the dried leaves of M. nivellei, consumed as tea in Saharan region, was characterized by UHPLC-PDA-HRMS. Fourteen compounds were characterized and, to confirm the proposed structures a preparative procedure followed by NMR spectroscopy was applied. Compound 3 (2-hydroxy-1,8-cineole disaccharide) was a never reported whereas a bicyclic monoterpenoid glucoside (2), two ionol glucosides (1 and 12), a tri-galloylquinic acid (4), two flavonol glycosides (5 and 9), and a tetra-galloylglucose (7), were reported in Myrtus spp. for the first time. Five flavonol O-glycosides (6, 8, 10-11, and 14) togheter a flavonol (13) were also identified. Quantitative determination of phenolic constituents from decoction and infusion has been performed by HPLC-UV-PDA. The phenolic content was found to be 150.5 and 102.6mg/g in decoction and infusion corresponding to 73.8 and 23.6mg/100mL of a single tea cup, respectively. Myricetin 3-O-ß-d-(6â³-galloyl)glucopyranoside (5), isomyricitrin (6) and myricitrin (8) were the compounds present in the highest concentration. The free-radical scavenging activities of teas and isolated compounds was measured by the DPPH assay and compared with the values of other commonly used herbal teas (green and black teas). Decoction displayed higher potency in scavenging free-radicals than the infusion and green and black teas.
Assuntos
Antioxidantes/química , Cicloexanóis/química , Monoterpenos/química , Myrtus/química , Chás de Ervas , Antioxidantes/isolamento & purificação , Cicloexanóis/isolamento & purificação , Eucaliptol , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonóis/química , Flavonóis/isolamento & purificação , Glucosídeos/química , Glucosídeos/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Estrutura Molecular , Monoterpenos/isolamento & purificação , Polifenóis/química , Polifenóis/isolamento & purificação , Terpenos/química , Terpenos/isolamento & purificaçãoRESUMO
AIM AND OBJECTIVE: Plasmodium knowlesi has been recently recognized as a human malarial parasite, particularly in the region of south-east Asia. Unlike human host, P. knowlesi cannot salvage pyrimidine bases and relies solely on nucleotides synthesized from de novo pyrimidine pathway. The enzymes involved in this are also unique in terms of their structure and function to its human counterpart. Thus, targeting Dihydroorotase, an enzyme involved in the pyrimidine biosynthesis, provides a promising route for novel drug development. MATERIALS AND METHODS: The 3D structure of P. knowlesi Dihydroorotase was predicted, refined and validated. Multiple docking was performed and the resultant complex was used for 3D structurebased pharmacophore modelling. A combinatorial library of 2,664,779 molecules was generated and used for structure based virtual screening. The stability of resultant compounds was checked using simulation studies. RESULTS: The modelled 3D structure of P. knowlesi Dihydroorotase enzyme is relaxed by running an MD simulation of 20 ns, and structure is validated by using Ramachandran plot and G-factor analysis. A five point based pharmacophore model was created and used as a query for screening in house database. The stability of two negatively charged compounds was studied, and ZINC22066495-DHOase complex was more stable throughout the simulation. CONCLUSION: The present study shows that ZINC22066495 compound has a high potential for disrupting P. knowlesi DHOase enzyme and may be used as a potential lead molecule for effective pyrimidine biosynthesis inhibition in P. knowlesi.
Assuntos
Antimaláricos/farmacologia , Cicloexanóis/farmacologia , Di-Hidro-Orotase/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Organofosfatos/farmacologia , Plasmodium knowlesi/efeitos dos fármacos , Plasmodium knowlesi/metabolismo , Pirimidinas/biossíntese , Antimaláricos/química , Cicloexanóis/química , Di-Hidro-Orotase/metabolismo , Avaliação Pré-Clínica de Medicamentos , Inibidores Enzimáticos/química , Ensaios de Triagem em Larga Escala , Humanos , Modelos Moleculares , Organofosfatos/química , Testes de Sensibilidade Parasitária , Pirimidinas/químicaRESUMO
This study evaluated the removal of filling material with ProTaper Universal Rotary Retreatment system (PTR) combined with solvents and the influence of solvents on the bond strength (PBS) of sealer to intraradicular dentin after canal reobturation. Roots were endodontically treated and distributed to five groups (n = 12). The control group was not retreated. In the four experimental groups, canals were retreated with PTR alone or in combination with xylol, orange oil, and eucalyptol. After filling material removal, two specimens of each group were analysed by SEM and µCT to verify the presence of filling remnants on root canal walls. The other roots were reobturated and sectioned in 1-mm-thick dentin slices that were subjected to the push-out test. Data were analysed by two-way ANOVA and Tukey's test (α = 0.05). SEM and µCT analysis revealed that all retreatment techniques left filling remnants on canal walls. The control group (3.47 ± 1.21) presented significantly higher (p < 0.05) PBS than the experimental groups. The groups retreated with PTR alone (2.59 ± 0.99) or combined with xylol (2.54 ± 0.77) and orange oil (2.32 ± 0.93) presented similar bond strength (p > 0.05), and differed significantly from the group with eucalyptol (1.89 ± 0.63). The solvents reduced the PBS of the sealer to dentin and no retreatment technique promoted complete removal of filling material.
Assuntos
Cavidade Pulpar/efeitos dos fármacos , Dentina/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/química , Preparo de Canal Radicular/métodos , Solventes/química , Análise de Variância , Cicloexanóis/química , Colagem Dentária , Instrumentos Odontológicos , Resinas Epóxi/química , Eucaliptol , Guta-Percha , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Monoterpenos/química , Óleos de Plantas/química , Reprodutibilidade dos Testes , Retratamento/instrumentação , Preparo de Canal Radicular/instrumentaçãoRESUMO
OBJECTIVES: The aim of this study was to evaluate the effect of different endodontic solvents on the microtensile bond strength (µTBS) of various adhesives to pulp chamber dentin. MATERIAL AND METHODS: A total of 120 human third molars were selected. Canals were prepared with the ProTaper Universal system and obturated. The access cavities were then restored with resin composite. After 1 week, a retreatment procedure was applied as follows: control, no solvent was applied to the pulp chamber and experimental groups, three different solvents (chloroform, eucalyptol, and orange oil) were applied to the pulp chamber for 2 min. The canal filling was removed and calcium hydroxide (Ca[OH]2) was placed into the canals. After 7 days, the Ca(OH)2 was removed from the canals and the canals were re-obturated. Teeth were then divided into three subgroups according to the adhesive used. The samples were restored with a nanohybrid resin composite using three different adhesives: Clearfil SE Bond (CSE), Adper Easy One (AEO), and Single Bond 2 (SB2). The samples were aged with thermocycling. Teeth were sectioned, and a total of 20 dentin sticks were obtained for each subgroup. µTBS testing was then performed. The debonded surfaces were evaluated using scanning electron microscopy (SEM) analysis. Data were analyzed using two-way ANOVA and Tukey's post hoc tests. RESULTS: Chloroform showed statistically lower mean µTBS values (14 ± 7.2 MPa) than control group did (19.2 ± 6.1 MPa) (p < 0.05). Orange oil (18.1 ± 6.3 MPa) and eucalyptol (16.9 ± 6.8 MPa) did not reduce the mean µTBS statistically (p > 0.05). Chloroform showed significantly lower bond strength for all adhesives (p < 0.05). Whereas orange oil did not reduce the mean µTBS values of all adhesive systems significantly (p > 0.05), eucalyptol reduced the µTBS values of all the groups, but the results were only statistically significant for SB2 (p < 0.05). CSE showed statistically higher bond strength (20.4 ± 6.8 MPa) than AEO (14.6 ± 5.3 MPa) and SB2 (16.3 ± 7.2 MPa) did (p < 0.05). There were no statistical differences between AEO and SB2 (p > 0.05). According to the SEM analysis of the debonded surfaces, adhesive failures were the most common type in all the groups, followed by mixed failures. CONCLUSIONS: While chloroform reduced the mean bond strength of the adhesive resins, orange oil did not affect the bond strength of the adhesives. The effect of eucalyptol on bond strength depended on the type of adhesive system. CLINICAL RELEVANCE: This study shows that endodontic solvents could affect the microtensile bond strength of adhesives to pulp chamber dentin.
Assuntos
Cavidade Pulpar , Adesivos Dentinários/química , Guta-Percha/química , Obturação do Canal Radicular/métodos , Clorofórmio/química , Cicloexanóis/química , Análise do Estresse Dentário , Eucaliptol , Microscopia Eletrônica de Varredura , Dente Serotino , Monoterpenos/química , Óleos de Plantas/química , Solventes/química , Resistência à TraçãoRESUMO
Abstract This study evaluated the removal of filling material with ProTaper Universal Rotary Retreatment system (PTR) combined with solvents and the influence of solvents on the bond strength (PBS) of sealer to intraradicular dentin after canal reobturation. Roots were endodontically treated and distributed to five groups (n = 12). The control group was not retreated. In the four experimental groups, canals were retreated with PTR alone or in combination with xylol, orange oil, and eucalyptol. After filling material removal, two specimens of each group were analysed by SEM and µCT to verify the presence of filling remnants on root canal walls. The other roots were reobturated and sectioned in 1-mm-thick dentin slices that were subjected to the push-out test. Data were analysed by two-way ANOVA and Tukey’s test (α = 0.05). SEM and µCT analysis revealed that all retreatment techniques left filling remnants on canal walls. The control group (3.47 ± 1.21) presented significantly higher (p < 0.05) PBS than the experimental groups. The groups retreated with PTR alone (2.59 ± 0.99) or combined with xylol (2.54 ± 0.77) and orange oil (2.32 ± 0.93) presented similar bond strength (p > 0.05), and differed significantly from the group with eucalyptol (1.89 ± 0.63). The solvents reduced the PBS of the sealer to dentin and no retreatment technique promoted complete removal of filling material.
Assuntos
Humanos , Cavidade Pulpar/efeitos dos fármacos , Dentina/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/química , Preparo de Canal Radicular/métodos , Solventes/química , Análise de Variância , Cicloexanóis/química , Colagem Dentária , Instrumentos Odontológicos , Resinas Epóxi/química , Guta-Percha , Teste de Materiais , Microscopia Eletrônica de Varredura , Monoterpenos/química , Óleos de Plantas/química , Reprodutibilidade dos Testes , Retratamento/instrumentação , Preparo de Canal Radicular/instrumentaçãoRESUMO
Patients with chronic diseases such as cardiovascular diseases, chronic respiratory diseases, and neurological diseases have been shown to benefit from treatments such as aromatherapy in addition to medication. Most chronic diseases are caused by chronic inflammation and oxidative stress as well as harmful factors. Eucalyptol (1,8-cineole), a terpenoid oxide isolated from Eucalyptus species, is a promising compound for treating such conditions as it has been shown to have anti-inflammatory and antioxidant effects in various diseases, including respiratory disease, pancreatitis, colon damage, and cardiovascular and neurodegenerative diseases. Eucalyptol suppresses lipopolysaccharide (LPS)-induced proinflammatory cytokine production through the action of NF-κB, TNF-α, IL-1ß, and IL-6 and the extracellular signal-regulated kinase (ERK) pathway, and reduces oxidative stress through the regulation of signaling pathways and radical scavenging. The effects of eucalyptol have been studied in several cell and animal models as well as in patients with chronic diseases. Furthermore, eucalyptol can pass the blood-brain barrier and hence can be used as a carrier to deliver drugs to the brain via a microemulsion system. In summary, the various biological activities of eucalyptol such as its anti-inflammatory and antioxidant properties, as well as its physicochemical characteristics, make this compound a potentially important drug for the treatment of chronic diseases.
Assuntos
Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Doença Crônica/tratamento farmacológico , Cicloexanóis/uso terapêutico , Descoberta de Drogas/métodos , Eucalyptus/química , Monoterpenos/uso terapêutico , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Cicloexanóis/química , Cicloexanóis/isolamento & purificação , Modelos Animais de Doenças , Eucaliptol , Humanos , Monoterpenos/química , Monoterpenos/isolamento & purificação , Fitoterapia , Plantas Medicinais , Transdução de Sinais/efeitos dos fármacosRESUMO
The external mucus surface of reef fish contains ultraviolet absorbing compounds (UVAC), most prominently Mycosporine-like Amino Acids (MAAs). MAAs in the external mucus of reef fish are thought to act as sunscreens by preventing the damaging effects of ultraviolet radiation (UVR), however, direct evidence for their protective role has been missing. We tested the protective function of UVAC's by exposing fish with naturally low, Pomacentrus amboinensis, and high, Thalassoma lunare, mucus absorption properties to a high dose of UVR (UVB: 13.4W∗m(-2), UVA: 6.1W∗m(-2)) and measuring the resulting DNA damage in the form of cyclobutane pyrimidine dimers (CPDs). For both species, the amount of UV induced DNA damage sustained following the exposure to a 1h pulse of high UVR was negatively correlated with mucus absorbance, a proxy for MAA concentration. Furthermore, a rapid and significant increase in UVAC concentration was observed in P. amboinensis following UV exposure, directly after capture and after ten days in captivity. No such increase was observed in T. lunare, which maintained relatively high levels of UV absorbance at all times. P. amboinensis, in contrast to T. lunare, uses UV communication and thus must maintain UV transparent mucus to be able to display its UV patterns. The ability to rapidly alter the transparency of mucus could be an important adaptation in the trade off between protection from harmful UVR and UV communication.
Assuntos
Dano ao DNA/efeitos da radiação , Peixes/metabolismo , Protetores Solares/metabolismo , Raios Ultravioleta , Aminoácidos/química , Aminoácidos/metabolismo , Animais , Cicloexanóis/química , Cicloexanóis/metabolismo , Muco/química , Muco/metabolismo , Protetores Solares/químicaRESUMO
BACKGROUND: Herbal liqueurs are alcoholic beverages produced by the maceration or distillation of aromatic and medicinal plants in alcohol, and are also highly valued for their medicinal properties. The process conditions, as well as the number and quantity of the plants employed, will have a great influence on the quality of the liqueur obtained. The aim of this research was to optimize these important variables. RESULTS: A Box-Benhken experimental design was used to evaluate the independent variables: alcohol content, amount of plant and time during the experimental maceration of plants in grape marc distillate. Four plants were assessed, with the main compound of each plant representing the dependent variable evaluated with respect to following the evolution of the maceration process. Bisabolol oxide A in Matricaria recutita L., linalool in Coriander sativum L. and eucalyptol in Eucalyptus globulus Labill. were quantified using a gas chromatography-flame ionization detector. Glycyrrhizic acid in Glycyrrhiza glabra L was determined using a high-performance liquid chromatography-diode array detector. Other dependent variables were also evaluated: total phenolic content, color parameters and consumer preference (i.e. appearance). CONCLUSION: The experimental designs allowed the selection of the optimal maceration conditions for each parameter, including the preference score of consumers: 70% (v/v) of ethanol, 40 g L-1 plant concentration and a maceration process of 3 weeks. © 2016 Society of Chemical Industry.
Assuntos
Extratos Vegetais/química , Plantas Medicinais/química , Vitis/química , Monoterpenos Acíclicos , Camomila , Coriandrum , Cicloexanóis/química , Eucaliptol , Eucalyptus , Sesquiterpenos Monocíclicos , Monoterpenos/química , Sesquiterpenos/químicaRESUMO
The rhizome of Hedychium spicatum has been widely used in traditional medicines. The present study deals with the evaluation of the cytotoxic potential of rhizome essential oils from four different regions of the Western Himalaya (India) along with comparative correlation analysis to characterise the bioactive cytotoxic component. The essential oils were coded as MHS-1, MHS-2, MHS-3 and MHS-4, and characterised using GC-FID and GC-MS. The main volatile compounds identified were 1,8-cineol, eudesmol, cubenol, spathulenol and α-cadinol. In vitro cytotoxic activities were assessed against human cancer cell lines such as, the lung (A549), colon (DLD-1, SW 620), breast (MCF-7, MDA-MB-231), head and neck (FaDu), and cervix (HeLa). MHS-4 is significantly active in comparison to other samples against all cancer cell lines. Sample MHS-4 has major proportion of monoterpene alcohol mainly 1,8-cineol. Principal components analysis was performed for the experimental results and all four samples were clustered according to their percentage inhibition at different doses.
Assuntos
Óleos Voláteis/química , Óleos de Plantas/química , Terpenos/química , Zingiberaceae/química , Linhagem Celular Tumoral , Cicloexanóis/química , Cicloexanóis/isolamento & purificação , Eucaliptol , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Índia , Monoterpenos/química , Monoterpenos/isolamento & purificação , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Plantas Medicinais/química , Análise de Componente Principal , Rizoma/química , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação , Sesquiterpenos de Eudesmano/química , Sesquiterpenos de Eudesmano/isolamento & purificação , Terpenos/isolamento & purificaçãoRESUMO
In the presence of a supported gold-palladium alloy nanoparticle catalyst (Au-Pd/Al2O3), various kinds of N-substituted anilines can be synthesized from non-aromatic compounds. The observed catalysis is truly heterogeneous, and Au-Pd/Al2O3 can be reused without a significant loss of its catalytic performance.
Assuntos
Ligas/química , Compostos de Anilina/síntese química , Ouro/química , Nanopartículas Metálicas/química , Paládio/química , Óxido de Alumínio/química , Aminas/química , Compostos de Anilina/química , Catálise , Cicloexanóis/química , Cicloexanonas/química , Estrutura Molecular , OxirreduçãoRESUMO
The chemical composition and antibacterial activity of Nepeta nuda (Lamiaceae) essential oil were examined, as well as the association between it and standard antibiotics: tetracycline and streptomycin. The antibacterial activities of 1,8-cineole, the main constituent of N. nuda oil, individually and in combination with standard antibiotics were also determined. The interactions of the essential oil and 1,8-cineole with antibiotics toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemoinformatics methods. Oxygenated monoterpenes were the most abundant compound class in the oil (57.8%), with 1,8-cineole (46.0%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotics. The combinations N. nuda oil-antibiotic and 1,8-cineole-antibiotic produced a predominantly antagonistic interactions. Chemoinformatics survey confirms the antagonistic interactions as a consequence of membrane potential/proton motive force dissipation. These data indicate cytochrome c oxidase as a target for 1.8-cineole toxicity action mechanisms.
Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Nepeta/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Óleos de Plantas/química , Óleos de Plantas/farmacologia , Bactérias/efeitos dos fármacos , Cicloexanóis/química , Cicloexanóis/farmacologia , Eucaliptol , Testes de Sensibilidade Microbiana , Monoterpenos/química , Monoterpenos/farmacologiaRESUMO
Matrix metalloproteinases play an important role in extracellular matrix remodeling. Excessive activity of these enzymes can be induced by UV light and leads to skin damage, a process known as photoaging. In this study, we investigated the collagenase inhibition potential of mycosporine-like amino acids, compounds that have been isolated from marine organisms and are known photoprotectants against UV-A and UV-B. For this purpose, the commonly used collagenase assay was optimized and for the first time validated in terms of relationships between enzyme-substrate concentrations, temperature, incubation time, and enzyme stability. Three compounds were isolated from the marine red algae Porphyra sp. and Palmaria palmata, and evaluated for their inhibitory properties against Chlostridium histolyticum collagenase. A dose-dependent, but very moderate, inhibition was observed for all substances and IC50 values of 104.0 µM for shinorine, 105.9 µM for porphyra, and 158.9 µM for palythine were determined. Additionally, computer-aided docking models suggested that the mycosporine-like amino acids binding to the active site of the enzyme is a competitive inhibition.