RESUMO
The present study was undertaken to isolate some compounds from methanol extract of Polygala tenuifolia and evaluate their immunostimulatory properties and antiviral activity using grass carp Ctenopharyngodon idella kidney (CIK) cells and GCRV. By applying insecticidal bioassay-guided, chromatography techniques and successive recrystallization, two purified compounds were obtained. The changes of expression of selected immune genes (Mx1, IL-1ß, TNFα, MyD88 and IgM) in C. idella kidney cell lines were evaluated after exposure to these isolated compounds. The results showed that compound 1 and 2 up-regulated to varying degrees of Mx1, IL-1ß, TNFα, and MyD88 in C. idella kidney cells. WST-8 kit assay verified the two compounds has no toxic effects on CIK cell, and furthermore, have in vitro antivirus activity. Especially, that there is keeping 79% cell viability when exposure to compound 2 (100 mg L(-1)). According to in vivo insecticidal assays against Dactylogyrus intermedius, compound 2 exhibited higher efficacy than compound 1, which was found to be 87.2% effective at the concentrations of 5 mg L(-1) and safe to goldfish (Carassius auratus). Besides, the purified compounds were identified by spectral data as: (1) 1,5-Anhydro-D-glucitol and (2) 3,4,5-trimethoxy cinnamic acid. Overall, the results indicate that bath administration of these compounds modulates the immune related genes in C. idella kidney cells and to some extent, eliminate the virus and parasitic infections.
Assuntos
Cinamatos/imunologia , Desoxiglucose/imunologia , Regulação da Expressão Gênica/efeitos dos fármacos , Extratos Vegetais/imunologia , Platelmintos/imunologia , Polygala/química , Reoviridae/imunologia , Animais , Carpas , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Cinamatos/isolamento & purificação , Cinamatos/farmacologia , Desoxiglucose/isolamento & purificação , Desoxiglucose/farmacologia , Regulação da Expressão Gênica/imunologia , Técnicas In Vitro , Metanol , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Platelmintos/efeitos dos fármacos , Reoviridae/efeitos dos fármacosRESUMO
Chemical and dermatotoxicological investigations of the natural and processed resin of the Mexican rubber plant, guayule (Parthenium argentatum), has established the presence of a sesquiterpene cinnamic acid ester (guayulin A) that is a potent elicitor of allergic contact dermatitis in experimental animals. The guayule contact allergen is comparable to the poison ivy skin allergens as an elicitor of dermatitis in sensitized guinea pigs.