RESUMO
Vanadium (V) is an ultratrace metal with the insulin-tropic properties and is often researched as the diabetes drug. However, in animals, V has been reported to have toxic effects on the development, immunity, oxidation-reduction equilibrium, gastrointestinal function, and so forth. Especially in poultry, supplementation of more than 10 mg of V/kg in the layer diets has been shown to adversely affect the egg production and egg quality. In this study, we supplemented 0 mg of V/kg, 5 mg of V/kg, and 10 mg of V/kg in the layer diets for 35 D and examined the quantitative proteomics of albumen for finding the possible target signaling pathway and mechanism of V action and made the preliminary verification. In contrast to the control group, V resulted in a significant drop in the albumen height, and in oviduct ampulla, the activity of total antioxidant capacity and glutathione peroxidase significantly decreased (P = 0.01, P = 0.02), the content of malonic dialdehyde significantly increased (P = 0.01), and the apoptosis rate significantly increased in the 5-mg V/kg and 10-mg V/kg treatment groups (P ï¼ 0.01). V affected 36 differentially accumulated proteins in albumen, with 23 proteins upregulated and 13 proteins downregulated. The expressions of innate protein albumen lysozyme (Q6LEL2), vitellogenin-2 (P02845), and the F1NWD0 protein in albumen belonged to the P53 family were significantly reduced, in contrast to the control (P < 0.05), and the expression of riboflavin-binding protein (P02752) was significantly improved (P < 0.05). The Hippo signaling pathway-fly, which is suitable for the key protein P53 as the most significantly affected network, might be important for discriminating V.
Assuntos
Ração Animal/análise , Clara de Ovo/análise , Proteoma , Vanádio/efeitos adversos , Animais , Galinhas , Dieta/veterinária , Proteínas do Ovo/química , Feminino , Oviductos , Transdução de SinaisRESUMO
BACKGROUND: Strontium is currently prescribed for patients with osteoporosis to increase bone density and reduce bone fractures but its relevance in animal nutrition is obscure. In order to investigate the effect of supplemental strontium and vitamin D3 on performance, egg quality and skeletal integrity in poultry a total of 108 laying hens, 99 weeks of age, were fed three levels of strontium (0, 500, 1000 mg kg(-1) ) and two levels of vitamin D3 (2500, 5,000 iu kg(-1)) over a 12-week period. RESULTS: There was an improvement (P < 0.05) in egg production and feed conversion efficiency with strontium at 500 mg kg(-1) and a significant increase in egg weight in those hens fed additional vitamin D3 . Supplemental strontium increased phosphorus, sodium and strontium retention in birds fed 2500 iu D3 kg(-1) but reduced phosphorus, sodium and strontium retention in birds fed 5000 iu D3 kg(-1), resulting in an interaction (P < 0.01) between strontium and vitamin D3 . Addition of 5000 iu D3 kg(-1) increased egg weight (P < 0.05); predominantly by increased albumen content (P < 0.05), whereas strontium supplementation reduced egg weight (P < 0.001). Similarly, 5000 iu kg(-1) D3 increased apparent metabolizable energy (P < 0.05); in contrast, strontium supplementation reduced (P < 0.05) apparent metabolizable energy. CONCLUSION: The addition of 500 mg kg(-1) strontium significantly improved egg production and feed efficiency; however, further investigation needs to be undertaken to refine the optimum level of strontium required to maximize hen performance. The interrelationship between strontium and vitamin D3 requires further exploratory study.
Assuntos
Galinhas/crescimento & desenvolvimento , Colecalciferol/administração & dosagem , Dieta/veterinária , Ovos , Qualidade dos Alimentos , Minerais/metabolismo , Estrôncio/administração & dosagem , Animais , Animais Endogâmicos , Densidade Óssea , Desenvolvimento Ósseo , Osso e Ossos/química , Osso e Ossos/metabolismo , Colecalciferol/efeitos adversos , Dieta/efeitos adversos , Casca de Ovo/química , Casca de Ovo/crescimento & desenvolvimento , Casca de Ovo/metabolismo , Clara de Ovo/análise , Gema de Ovo/química , Gema de Ovo/metabolismo , Ovos/análise , Ingestão de Energia , Metabolismo Energético , Feminino , Minerais/administração & dosagem , Minerais/análise , New South Wales , Ovalbumina/análise , Ovalbumina/metabolismo , Oviposição , Estrôncio/efeitos adversos , TíbiaRESUMO
Soy isoflavones have been associated with several beneficial effects of soy in human diets. However, most soy is consumed by livestock in the Western countries. It is possible that isoflavones could be transferred and/or accumulated into animal products, which could become additional sources of dietary isoflavones for humans. Our objectives were to determine whether dietary isoflavone genistein could be transferred and/or accumulated into the eggs of Japanese quail (Coturnix japonica) and how the supplementation dosage and glycosylation of the isoflavone would affect this transfer. Adult reproductive female Japanese quail were randomly assigned to treatment groups that received encapsulated 50 or 100 mg genistein or 80 mg genistin per day (four quail per treatment) for 5 days. A control group (two quail) received placebo capsules. Eggs were collected prior to treatment and then daily for 15 days. The egg, separated into yolk and white, and pulverized quail diet were extracted in 80% methanol for 2 h and either centrifuged or filtered before evaporation of the solvent. The extracts were redissolved in 16% acetonitrile for high-performance liquid chromatography (HPLC) analyses. Genistein and genistein metabolites were detected in the egg yolks of treated quail. Trace concentrations of genistein were detected in the control group, due to the presence of genistein derivatives in the diet. Neither genistein nor its metabolites were found in egg white. Levels of genistein in the eggs increased significantly from the 3rd day of supplementation and reached the maximum about 2 days after the supplementation stopped. The higher dose of genistein supplementation resulted in higher genistein concentrations in egg yolks. Glycosylation decreased the transfer and accumulation of genistein into the egg yolks.
Assuntos
Coturnix/metabolismo , Ovos/análise , Genisteína/administração & dosagem , Animais , Cromatografia Líquida de Alta Pressão , Suplementos Nutricionais , Clara de Ovo/análise , Gema de Ovo/química , Feminino , Genisteína/análise , Genisteína/química , Glicosilação , PlacebosRESUMO
Effects of dietary conjugated linoleic acids (CLAs) and docosahexaenoic acid (DHA) on the fatty acid composition of different egg compartments after storage were studied. Four dietary treatments [supplemented with safflower oil (SAFF, control group), DHA, CLAs plus DHA (CAD), and CLAs alone] were administered to Single Comb White Leghorn (SCWL) laying hens. Eggs from the different treatment groups were collected and stored for 10 weeks at 4 degrees C before analysis. Fatty acids from the yolk (yolk granules and plasma), egg albumen, and vitelline membrane were analyzed by gas chromatography. The yolk of eggs from hens given CLAs had significantly higher amounts of saturated fatty acids, typically 16:0 and 18:0, but lower amounts of polyunsaturated fatty acids (PUFAs) compared to eggs from the control group (SAFF). CLA content was highest in the yolk and present in both neutral and polar lipids, with the greatest concentrations in neutral lipids. DHA was incorporated mainly into yolk polar lipids. Lipids in yolk plasma and granules contained similar amounts of CLAs. The fatty acid compositions of vitelline membrane and egg albumen mirrored that of the egg yolk. CLA supplementation resulted in hard and rubbery yolks when compared to hard-cooked eggs from the control group. This study showed that feeding CLAs to hens led to accumulation of the isomers in polar and neutral lipids of the egg yolk and that these isomers migrated into egg albumen. Because the sensory properties of hard-cooked eggs were negatively affected by the enrichment of a mixture of CLA isomers in this study, further research should be conducted to evaluate how the different isomers alter the properties of egg yolk and albumen so that the quality of designed eggs containing CLAs and DHA can be improved.
Assuntos
Galinhas/fisiologia , Gorduras na Dieta/administração & dosagem , Gema de Ovo/química , Ácidos Graxos/análise , Ácidos Linoleicos Conjugados/administração & dosagem , Ovalbumina/química , Animais , Cromatografia Gasosa , Ácidos Docosa-Hexaenoicos/administração & dosagem , Clara de Ovo/análise , Feminino , Ácidos Linoleicos Conjugados/análise , Lipídeos/análise , Óleo de Cártamo/administração & dosagem , Membrana Vitelina/químicaRESUMO
We studied the effect of dietary source (organic or inorganic) and level of Se on the Se uptake of chick embryos. After receiving a low-Se diet for 16 wk, 126 Leghorn laying hens were randomly assigned to one of seven dietary treatments. Treatments consisted of feeding a low-Se basal diet alone or with one of three levels of added Se (0.1,0.2, or 0.3 mg/kg Se) supplied by sodium selenite or Se-enriched yeast. Fertile eggs were collected after 33 d of feeding the experimental diets. Eggs were subjected to no incubation or incubation for 5, 10, 15, or 20 d. Non-incubated eggs were separated, and the yolk and albumen were assayed separately for Se. Incubated eggs were separated into the embryo and extra-embryonic portions, which were assayed separately for Se. Se concentrations of the yolk and albumen were significantly different among dietary treatments. Compared with eggs from hens fed sodium selenite, yolk and albumen Se concentrations were higher in eggs from hens fed Se yeast. Embryonic and extra-embryonic Se concentrations were higher in eggs from hens fed Se yeast than eggs from hens fed sodium selenite. The largest increase in embryonic Se concentration was observed during Days 10 to 15 of incubation. It was concluded that Se source and dietary inclusion level influenced the Se concentration of portions of developing embryonated eggs and that embryonic Se concentration changed during incubation.
Assuntos
Embrião de Galinha/metabolismo , Galinhas/metabolismo , Dieta , Selênio/administração & dosagem , Selênio/farmacocinética , Animais , Embrião de Galinha/química , Clara de Ovo/análise , Gema de Ovo/química , Feminino , Selênio/análise , Selenito de Sódio/administração & dosagem , Fatores de Tempo , Fermento SecoRESUMO
The effect of phenyl mercury with and without selenium on the egg production of laying hens and on the fertility, hatchability and properties of eggs was studied. Mercury was administered via the feed at dosages of 5 ppm, 30 ppm, and 30 ppm Hg + 4 ppm Se, for 56 days. After two months, egg production decreased by 8.18% and 7.74% in hens fed 30 ppm Hg, and 30 ppm Hg + 4 ppm Se, respectively. Egg weight decreased in all experimental groups. In comparison to the controls, these results were highly significant (P < 0.01) in hens fed 30 ppm Hg and 30 ppm Hg + 4 ppm Se and significant (P < 0.05) between hens fed 5 ppm Hg and 30 ppm Hg. Fertility rate and hatchability were not affected. Mercury exposure did not affect egg shape, egg-white height, egg-shell hardness or yolk colour. Both egg-shell thickness and weight decreased in all experimental groups. In the group supplemented with selenium there was a nonsignificant improvement in egg production, hatchability and all qualitative properties of eggs in comparison with the group without selenium supplementation. Residual mercury levels in egg yolk greatly surpassed the level found in the egg white: the highest values were measured in the group fed 30 ppm Hg. The addition of selenium had a protective effect upon residual Hg deposits in the yolk, but not in the egg-white.
Assuntos
Galinhas/fisiologia , Fungicidas Industriais/farmacologia , Compostos de Fenilmercúrio/farmacologia , Reprodução/efeitos dos fármacos , Administração Oral , Animais , Combinação de Medicamentos , Casca de Ovo/química , Casca de Ovo/fisiologia , Clara de Ovo/análise , Gema de Ovo/química , Gema de Ovo/fisiologia , Ovos/análise , Feminino , Fertilização/efeitos dos fármacos , Fertilização/fisiologia , Fungicidas Industriais/administração & dosagem , Fungicidas Industriais/análise , Oviposição/efeitos dos fármacos , Oviposição/fisiologia , Compostos de Fenilmercúrio/administração & dosagem , Compostos de Fenilmercúrio/análise , Reprodução/fisiologia , Selênio/farmacologiaRESUMO
Regional distribution of selenium (Se) in Chile was investigated by using the concentration of Se in hens' eggs as a monitor. Forty-one locations along the entire length of the country were sampled. Average (+/- SD) egg-white Se content (mg/kg dry) was 0.79 +/- 0.41, range 0.22-2.23. Corresponding yolk Se values were, mean 0.81 +/- 0.43, (mg/kg dry) range 0.26-2.23. Locations grouped in five main areas, according to their geographic-climatic characteristics, showed significant differences regarding both egg-white Se and yolk Se. Analyzed dietary Se intake from two distinct areas reflected the trends observed in the Se content of egg fractions from such regions. These data support the utilization of the concentration of Se in hens' eggs as a useful monitor of dietary selenium consumed by selected populations.
Assuntos
Clara de Ovo/análise , Gema de Ovo/química , Ovos/análise , Selênio/análise , Animais , Galinhas , Chile , Gema de Ovo/metabolismo , Monitoramento Ambiental , FemininoRESUMO
The relationship between selenium and mercury in marine waders from the Wadden Sea (known to have high tissue selenium concentrations), was investigated in the framework of the possibility that high mercury concentrations may have induced parallel selenium accumulation to detoxify the mercury. The selenium and mercury concentrations are shown to be negatively correlated in both the liver and kidneys of these birds. In the tissues and red blood cells of oystercatchers, positive correlations between the two elements are found. The atom increment Se/Hg ratio in the pooled oystercatcher tissue and red cell data is 32:1. This ratio greatly exceeds the 1:1 ratio found when selenium is accumulated to detoxify mercury. Furthermore, breeding females are able to excrete mercury independently of selenium from the red blood cells, probably into the eggs; the whole egg mercury concentrations of the local breeding populations are low. From these results it is concluded that the high selenium concentrations in waders do not have their origin in elevated levels of mercury.
Assuntos
Aves , Mercúrio/análise , Selênio/análise , Animais , Clara de Ovo/análise , Eritrócitos/metabolismo , Feminino , Rim/metabolismo , Fígado/metabolismo , Masculino , Mercúrio/farmacocinética , Água do Mar , Selênio/farmacocinética , Distribuição TecidualRESUMO
Two experiments were conducted with Large White Turkey hens in individual cages to determine the value of supplemental biotin for increasing hatchability. No differences were observed for 7-day embryo deaths between treatments in both experiments. In the last two-thirds of the production cycle in both experiments, eggs from hens fed 520 micrograms and 623 micrograms/kg had the fewest embryonic deaths during Days 7 to 28 of incubation. Concentrations of biotin in egg albumen increased with incremental dietary biotin levels, but egg yolk concentrations were stable. About 38 micrograms of biotin per egg (82 g) produced highest embryo survival. Regression analysis, based on average percentage hatchability at the treatment levels for both experiments, revealed no hatchability response for Period 1 (first third of production cycle) from biotin. However, the dietary biotin level for hatchability increased with maternal age, which ranged from 500 to 800 micrograms/kg for Periods 2 (second third of production cycle) and 3 (last third of production cycle), respectively.
Assuntos
Biotina/farmacologia , Perus/embriologia , Fatores Etários , Ração Animal , Animais , Biotina/administração & dosagem , Biotina/análise , Clara de Ovo/análise , Gema de Ovo/química , Feminino , Alimentos Fortificados , Oviposição/efeitos dos fármacos , Distribuição Aleatória , Análise de Regressão , Perus/fisiologiaRESUMO
Chicken tissues were intrinsically labeled with a stable isotope of selenium (76Se) and were evaluated for use in a human feeding study. Laying hens were fed a low Se (0.06 ppm) basal diet for 39 days and then fed the basal diet supplemented with 0.3 ppm enriched 76Se (as selenite) for 35 days. Incorporation of 76Se into samples was determined by use of a double isotope dilution technique and a combined gas chromatography/mass spectrometry analysis. The 76Se content of the basal diet was increased by a factor of 9.7 with the addition of the enriched stable isotope. This maximal level of enrichment was approached in egg yolk (9.5-fold) and liver (9.0-fold). Enrichment was lower in egg white (7.2-fold) and breast meat (5.0-fold). Level of enrichment in a given tissue reflected both the turnover rate of the tissue and its natural selenium content. Selenium-depleted laying hens continuously fed 76Se at the 0.3 ppm level produced egg yolks and livers that were enriched sufficiently with the stable isotope for use in a human metabolic study.
Assuntos
Galinhas/metabolismo , Ovos/análise , Selênio/metabolismo , Animais , Clara de Ovo/análise , Gema de Ovo/análise , Feminino , Isótopos , Fígado/metabolismo , Músculos/metabolismo , OviposiçãoRESUMO
Female chickens were fed graded levels of sodium selenite to determine at what level a selenium toxicity occurred. In the first experiment a basal diet was supplemented with 0, 0.1, 1.0, 3.0, and 5.0 ppm of selenium. These levels had no effect on egg production, egg weight or fertility of the eggs. Hatchability of fertile eggs was significantly decreased by 5 ppm of dietary selenium. In the second experiment a basal diet was supplemented with 0, 5.0, 7.0, and 9.0 ppm of selenium. Egg weight and hatchability were significantly decreased by 7 and 9 ppm, and egg production was decreased by 9 ppm of selenium in the diet. When selenite was added to the diet, there was a lag of 2 to 3 weeks before the selenium content of the egg reflected the content of the diet. As long as the selenium content of the diet remained the same, egg selenium remained the same. When selenium was no longer supplemented, egg selenium content decreased. Two weeks after cessation of selenite supplementation, egg selenium was markedly reduced. Four weeks after cessation of selenite supplementation, egg selenium levels approached those of birds fed a basal diet continuously.
Assuntos
Galinhas , Selênio/toxicidade , Animais , Embrião de Galinha/efeitos dos fármacos , Clara de Ovo/análise , Gema de Ovo/análise , Feminino , Oviposição/efeitos dos fármacos , Selênio/análise , Distribuição TecidualAssuntos
Galinhas/metabolismo , Coturnix/metabolismo , Ovos/análise , Compostos de Metilmercúrio/metabolismo , Codorniz/metabolismo , Selênio/metabolismo , Animais , Encéfalo/metabolismo , Interações Medicamentosas , Clara de Ovo/análise , Gema de Ovo/análise , Feminino , Hematócrito , Fígado/metabolismo , Compostos de Metilmercúrio/administração & dosagem , Selênio/administração & dosagemRESUMO
Practical diets containing various selenium levels, with and without selenite supplementation, were fed to hens. Eggs were then collected over a 14-day period to determine how quickly changes in dietary selenium affected egg white and yolk selenium. Changes in egg white selenium content were rapid and essentially completed seven days after changing the selenium content of a practical diet. Changes in egg yolk were not yet completed by 14 days. When selenium from practical feedstuffs was fed, the selenium content of dried egg white was about equal to or greater than the selenium content of dried egg yolk. When selenite was fed, the selenium content of dried yolk was higher. Feeding selenomethionine resulted in more selenium in egg white than in egg yolk. Feeding selenocystine resulted in more selenium in egg yolk than egg white, a pattern similar to that from feeding selenite. The data suggest that selenocystine is not incorporated into protein but is metabolized to an inorganic selenium compound.