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1.
J Asian Nat Prod Res ; 24(5): 468-482, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35118925

RESUMO

Six new secondary metabolites, including two new nor-triterpenes (1 and 2), one new sesquiterpene (4), two new α-pyrone derivatives (6 and 7), and one new natural product (5) along with two known compounds (3 and 8) were isolated from an endophytic fungus Colletotrichum gloeosporioides obtained from a toxic medicinal plant Tylophora ovata. Their structures were elucidated by spectroscopic data analyses, while their absolute configurations were determined by CD and X-ray diffraction analyses. The in vitro anti-inflammatory activities of these compounds were evaluated.


Assuntos
Colletotrichum , Plantas Medicinais , Colletotrichum/química , Colletotrichum/metabolismo , Endófitos/química , Estrutura Molecular , Tylophora
2.
Fitoterapia ; 141: 104474, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31927010

RESUMO

Two novel eremophylane acetophenone conjugates, colletotricholides A (1) and B (2), were isolated from the solid fermentation cultures of an endophytic fungus Colletotrichum gloeosporioides XL1200 isolated from the aerial parts of Salvia miltiorrhiza. The chemical structures of 1-2 were characterized by extensive spectroscopic methods and single-crystal X-ray crystallography. Structurally, compounds 1-2 are two unusual eremophylane acetophenone conjugates originating from the hybrid pathways of polyketide synthase and sesquiterpene synthase. In addition, compounds 1-2 were inactive against tested pathogens.


Assuntos
Acetofenonas/química , Colletotrichum/química , Salvia miltiorrhiza/microbiologia , Antibacterianos/química , Antibacterianos/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Bactérias/efeitos dos fármacos , Endófitos , Fungos/efeitos dos fármacos , Modelos Moleculares , Estrutura Molecular , Componentes Aéreos da Planta/microbiologia
3.
Fitoterapia ; 141: 104465, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31870947

RESUMO

Seven new dimeric tryptophol-related alkaloids (1-4, 5a, 5b, and 6) were isolated from solid cultures of the endophytic fungus Colletotrichum sp. SC1355. The structures and absolute configurations of these compounds were determined by NMR spectroscopic analyses in combination with quantum chemical calculations of NMR (GIAO) shifts and ECD spectra. This is the first report of fungus-derived tryptophol dimers. In addition, the isolated compounds were evaluated for acetylcholinesterase (AchE) inhibitory activity.


Assuntos
Colletotrichum/química , Indóis/química , Estrutura Molecular
4.
Zhongguo Zhong Yao Za Zhi ; 44(3): 460-464, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30989908

RESUMO

In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.


Assuntos
Alternaria/patogenicidade , Ascomicetos/patogenicidade , Chelidonium/microbiologia , Colletotrichum/isolamento & purificação , Fusarium/isolamento & purificação , Antibiose , Colletotrichum/química , Endófitos/química , Endófitos/isolamento & purificação , Fusarium/química
5.
Fitoterapia ; 123: 29-34, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28962943

RESUMO

Citrinal B, a tricyclic compound from endophytic fungus Colletotrichum capsici in our previous studies, exhibited significant inhibitory activity against 11ß-hydroxysteroid dehydrogenase type 1 (11 ß-HSD1) in vitro and showed strong binding affinity to 11ß-HSD1. Moreover, citrinal B treatments decreased the lipid droplet accumulation associate with the inhibition of 11ß-HSD1 expression in differentiate induced 3T3-L1 preadipocytes. Furthermore, the molecular docking demonstrated that citrinal B coordinated in the active site of 11ß-HSD1 is essential for the ability of diminishing the enzyme activity.


Assuntos
11-beta-Hidroxiesteroide Desidrogenase Tipo 1/antagonistas & inibidores , Colletotrichum/química , Inibidores Enzimáticos/química , Compostos Heterocíclicos com 3 Anéis/química , Células 3T3-L1 , Animais , Inibidores Enzimáticos/isolamento & purificação , Células Hep G2 , Compostos Heterocíclicos com 3 Anéis/isolamento & purificação , Humanos , Metabolismo dos Lipídeos/efeitos dos fármacos , Camundongos , Simulação de Acoplamento Molecular
6.
Microb Ecol ; 74(1): 54-61, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28058469

RESUMO

Fungal endophytes are microorganisms that are well-known for producing a diverse array of secondary metabolites. Recent studies have uncovered the bioprospecting potential of several plant endophytic fungi. Here, we demonstrate the presence of highly bioactive fungal endophytic species in Aquilaria subintegra, a fragrant wood plant collected from Thailand. Thirty-three fungal endophytic strains were isolated and further identified to genus level based on morphological characteristics. These genera included Colletotrichum, Pestalotiopsis, Fusarium, Russula, Arthrinium, Diaporthe and Cladosporium. All strains were cultured on potato dextrose broth for 30 days prior to partitioning with ethyl acetate. The volatile compounds of all extracts were investigated by gas chromatography-mass spectrometry (GC-MS). Four strains-Arthrinium sp. MFLUCC16-0042, Colletotrichum sp. MFLUCC16-0047, Colletotrichum sp. MFLUCC16-0048 and Diaporthe sp. MFLUCC16-0051-produced a broad spectrum of volatile compounds, including ß-agarofuran, α-agarofuran, δ-eudesmol, oxo-agarospirol, and ß-dihydro agarofuran. These compounds are especially important, because they greatly resemble those originating from the host-produced agarwood oil. Our findings demonstrate the potential of endophytic fungi to produce bioactive compounds with applications in perfumery and cosmetic industries. Antioxidant activity of all extracts was also evaluated by using 2,2-diphenyl-2-picrylhydrazyl radical scavenging assays. The ethyl acetate extract of Diaporthe sp. MFLUCC16-0051 demonstrated superior antioxidant capacity, which was comparable to that of the gallic acid standard. Our results indicate that the MFLUCC16-0051 strain is a resource of natural antioxidant with potential medicinal applications.


Assuntos
Ascomicetos/química , Colletotrichum/química , Endófitos/química , Óleos de Plantas/análise , Thymelaeaceae/microbiologia , Compostos Orgânicos Voláteis/análise , Cromatografia Gasosa-Espectrometria de Massas , Tailândia
7.
Fitoterapia ; 113: 158-63, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27520493

RESUMO

Four novel lactams, colletotrilactam A-D (1-4), along with six known compounds (5-10) were isolated from the culture broth of Colletotrichum gloeosporioides GT-7, a fungal endophyte of Uncaria rhynchophylla. The structures of these compounds were elucidated by comprehensive NMR spectroscopy. Isolates were tested for monoamine oxidase (MAO) inhibitory activity and compound 9 showed potent MAO inhibitory activity with IC50 value of 8.93±0.34µg/mL, when the IC50 value of iproniazid as a standard was 1.80±0.5µg/mL.


Assuntos
Colletotrichum/química , Lactamas/química , Inibidores da Monoaminoxidase/química , Uncaria/microbiologia , Endófitos/química , Lactamas/isolamento & purificação , Estrutura Molecular , Monoaminoxidase/metabolismo , Inibidores da Monoaminoxidase/isolamento & purificação
8.
Drug Res (Stuttg) ; 66(10): 527-531, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27463031

RESUMO

Background: Respiratory syncytial virus (RSV) is known to cause severe respiratory infections particularly in infants younger than 2 years of age. The only approved drug, ribavirin, is expensive and is not likely to improve therapeutic outcome, thereby necessitating the search for safer and more potent alternatives from natural sources such as endophytic fungi. The present study aimed to investigate the anti-RSV activity of compounds from endophytic fungi. Methods: Two endophytic fungi Colletotrichum gloeosporioides and Pestalotiopsis thea were isolated from the fresh leaves of the host Nigerian plants Anthocleista djalonensis and Fagara zanthoxyloides, respectively. After fermentation in solid rice media, C. gloeosporioides afforded 4 known compounds 4-hydroxybenzoic acid (1), vanillic acid (2), ferulic acid (3) and Nb-acetyltryptamine (4) while P. thea afforded 3 known compounds chloroisosulochrin (5), ficipyrone A (6) and pestheic acid (7). The compounds were investigated for their anti-RSV activity using the HEP-2 cell lines and ribavirin as the standard drug. Results: Compound 5 was found to show the strongest inhibition of the RSV with IC50 of 4.22±1.03 µM (ribavirin 4.91±1.85 µM). Other compounds showed moderate inhibition of the virus (IC50 ranging from 45.00±0.98 to 259.23±2.36 µM). Conclusion: The results of the present study have shown that chloroisosulochrin (5), isolated from an endophytic fungus P. thea, possesses strong activity against RSV.


Assuntos
Antivirais/isolamento & purificação , Antivirais/farmacologia , Colletotrichum/química , Testes de Sensibilidade Microbiana , Plantas Medicinais/química , Vírus Sinciciais Respiratórios/efeitos dos fármacos , Xylariales/química , Células Cultivadas , Humanos , Nigéria
9.
Acta amaz ; 46(1): 69-72, jan./mar. 2016. ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1455283

RESUMO

Endophytic fungi are fungi that colonize internal tissues of plants. There are few studies of compounds isolated from endophytic fungi of Amazon plants. Thus, the aim this study was the isolation and structural identification of sitosterol (1), stigmasterol (2), sitostenone (3), squalene (4), ergosterol (5) and ergosterol peroxide (6) from fungus Colletotrichum gloeosporioidesisolated as endophytic from Virola michelli, a typical Amazon plant, used in folk medicine against skin infection. Compounds were isolated by chromatography column on silica and identified by 1H and 13C NMR and MS. The presence of phytosterols in fungi is rare and this is the first report of the isolation of the phytosterols sitosterol, stigmasterol and sitostenone from the genus Colletotrichum.


Fungos endofíticos são fungos que colonizam os tecidos internos das plantas. Existem poucos estudos de compostos isolados de fungos endofíticos de plantas da Amazônia. Assim, o objetivo deste estudo foi o isolamento e identificação estrutural de sitosterol (1), estigmasterol (2), sitostenona (3), esqualeno (4), ergosterol (5) e peroxido de ergosterol (6) do fungo Colletotrichum gloeosporioidesisolado como endofítico de Virola michelli, uma planta típica da Amazônia, usada na medicina popular no combate a infecções de pele. Os compostos foram isolados por cromatografia em coluna de sílica e identificados por RMN 1H e 13C e EM. A presença de fitoesteróis em fungos é rara e este é o primeiro relato do isolamento dos fitoesteróides sitosterol, estigmasterol e sitostenona do gêreno Colletotrichum.


Assuntos
Animais , Colletotrichum/química , Endófitos , Fitosteróis/isolamento & purificação , Myristicaceae , Esteroides , Fungos
10.
J Nat Prod ; 79(4): 704-10, 2016 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-26905687

RESUMO

Three new compounds, colletotrichones A-C (1-3), and one known compound, chermesinone B (4a), were isolated from an endophytic fungus, Colletotrichum sp. BS4, harbored in the leaves of Buxus sinica, a well-known boxwood plant used in traditional Chinese medicine (TCM). Their structures were determined by extensive spectroscopic analyses including 1D and 2D NMR, HRMS, ECD spectra, UV, and IR, as well as single-crystal X-ray diffraction, and shown to be azaphilones sharing a 3,6a-dimethyl-9-(2-methylbutanoyl)-9H-furo[2,3-h]isochromene-6,8-dione scaffold. Owing to the remarkable antibacterial potency of known azaphilones coupled to the usage of the host plant in TCM, we evaluated the antibacterial efficacy of the isolated compounds against two commonly dispersed environmental strains of Escherichia coli and Bacillus subtilis, as well as against two human pathogenic clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa. Compound 1 exhibited marked antibacterial potencies against the environmental strains that were comparable to the standard antibiotics. Compound 3 was also active against E. coli. Finally, compound 2a exhibited the same efficacy as streptomycin against the clinically relevant bacterium S. aureus. The in vitro cytotoxicity of these compounds on a human acute monocytic leukemia cell line (THP-1) was also assessed. Our results provide a scientific rationale for further investigations into endophyte-mediated host chemical defense against specialist and generalist pathogens.


Assuntos
Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Benzopiranos/isolamento & purificação , Benzopiranos/farmacologia , Colletotrichum/química , Pigmentos Biológicos/isolamento & purificação , Pigmentos Biológicos/farmacologia , Antibacterianos/química , Bacillus subtilis/efeitos dos fármacos , Benzopiranos/química , Buxus/microbiologia , Medicamentos de Ervas Chinesas , Escherichia coli/efeitos dos fármacos , Humanos , Leucemia Monocítica Aguda/tratamento farmacológico , Testes de Sensibilidade Microbiana , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Pigmentos Biológicos/química , Folhas de Planta/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Estreptomicina/farmacologia
11.
Analyst ; 140(9): 3098-106, 2015 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-25790802

RESUMO

Colletotrichum coccodes (C. coccodes) is a pathogenic fungus that causes anthracnose on tomatoes and black dot disease in potatoes. It is considered as a seed tuber and soil-borne pathogen that is difficult to control. C. coccodes isolates are classified into Vegetative Compatibility Groups (VCGs). Early classification of isolates into VCGs is of great importance for a better understanding of the epidemiology of the disease and improving its control. Moreover, the differentiation among these isolates and the assignment of newly-discovered isolates enable control of the disease at its early stages. Distinguishing between isolates using microbiological or genetic methods is time-consuming and not readily available. Our results show that it is possible to assign the isolates into their VCGs and to classify them at the isolate level with a high success rate using Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA).


Assuntos
Colletotrichum/química , Doenças das Plantas/microbiologia , Solanum lycopersicum/microbiologia , Solanum tuberosum/microbiologia , Espectrofotometria Infravermelho , Colletotrichum/classificação , Colletotrichum/isolamento & purificação , Análise Discriminante , Análise de Componente Principal
12.
Molecules ; 19(11): 19243-52, 2014 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-25421415

RESUMO

In this study, eight endophytic fungi were isolated from the leaves, stems and roots of Michelia champaca. The isolates were screened and evaluated for their antifungal, anticancer and acetylcholinesterase (AChE) inhibitory activities. All of the extracts exhibited potent activity against two evaluated phytopathogenic fungi. Chemical investigation of EtOAc extracts of the endophytic fungus Colletotrichum gloeosporioides resulted in the isolation of one new compound, 2-phenylethyl 1H-indol-3-yl-acetate (1), and seven known compounds: uracil (2), cyclo-(S*-Pro-S*-Tyr) (3), cyclo-(S*-Pro-S*-Val) (4), 2(2-aminophenyl)acetic acid (5), 2(4-hydroxyphenyl)acetic acid (6), 4-hydroxy- benzamide (7) and 2(2-hydroxyphenyl)acetic acid (8). All of the compound structures were elucidated using 1D and 2D NMR and MS analyses. The antifungal and AChE inhibitory activities of compounds 1-8 were evaluated in vitro. Compound 1 exhibited promising activity against Cladosporium cladosporioides and C. sphaerospermum that was comparable to that of the positive control nystatin.


Assuntos
Antifúngicos/química , Antifúngicos/farmacologia , Colletotrichum/química , Fungos/efeitos dos fármacos , Magnoliaceae/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacologia , Cladosporium/efeitos dos fármacos , Testes de Sensibilidade Microbiana/métodos , Folhas de Planta/química , Raízes de Plantas/química , Caules de Planta/química
13.
Fitoterapia ; 83(4): 737-41, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22417867

RESUMO

Six aromatic compounds were isolated from the endophytic fungus Colletotrichum sp. of Cephalotaxus hainanensis Li [Cephalotaxus mannii Hook. f.]. Their structures were determined on the basis of chemical and spectroscopic methods. The compound 2 was the enantiomer of compound 1. The compound 4 was the epimeride of the compound 3. The compounds 1, 2, 4 and 5 were evaluated for cytostatic activity against HL-60 and PC-3 cells.


Assuntos
Produtos Biológicos/isolamento & purificação , Cephalotaxus/microbiologia , Colletotrichum/química , Produtos Biológicos/química , Produtos Biológicos/farmacologia , Linhagem Celular Tumoral , Endófitos/química , Células HL-60 , Humanos , Estrutura Molecular , Estereoisomerismo
14.
Nat Prod Commun ; 6(8): 1131-2, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21922917

RESUMO

Two compounds, apigenin-8-C-beta-D-glucopyranoside and 2-(hydroxymethylthio)ethanol, were extracted from the fermentation products of a strain of endophytic fungus, Colletotrichum sp. NTB-2, isolated from the leafstalk of Ginkgo biloba. The structures of the two compounds were determined on the basis of extensive spectroscopic analysis, including 1D and 2D NMR spectral data. The compounds wereobtained from microorganisms for the first time.


Assuntos
Álcoois/química , Apigenina/química , Colletotrichum/química , Ginkgo biloba/microbiologia , Glicosídeos/química , Piranos/química , Compostos de Sulfidrila/química , Estrutura Molecular
15.
Nat Prod Commun ; 4(11): 1449-54, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19967971

RESUMO

The new (22E,24R)-3-acetoxy-19(10-->6)-abeo-ergosta-5,7,9,22-tetraen-3beta-ol (1) and the known (22E,24R)-19(10-->6)-abeo-ergosta-5,7,9,22-tetraen-3beta-ol (2), two interesting ergosteroids with rare aromatized ring B, together with seven known derivatives, namely (22E,24R)-ergosta-5,7,22-trien-3beta-ol (3), (22E,24R)-ergosta-4,7,22-trien-3-one (4), (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one (5), (22E,24R)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol (6), (22E,24R)-ergosta-7,22-dien-3beta,5alpha,6beta-triol (7), (22E,24R)-6-acetoxy-ergosta-7,22-dien-3beta,5alpha,6beta-triol (8), and (22E,24R)-3,6-diacetoxy-ergosta-7,22-dien-3beta,5alpha,6beta-triol (9), were isolated from Colletotrichum sp., an endophytic fungus isolated from Ilex canariensis from Gomera. The structures of these compounds were elucidated by detailed spectroscopic analysis, comparison with reported data, and chemical interconversion. The isolation of these metabolites not only displays a beautiful array of chemical diversity, but also gives insight into the biosynthetic interconnections. Preliminary studies showed antimicrobial activity of these compounds against the fungus Microbotryum violaceum, the alga Chlorella fusca, and the bacteria Escherichia coli and Bacillus megaterium.


Assuntos
Colletotrichum/química , Esteroides/química , Acetilação , Antibacterianos/biossíntese , Antibacterianos/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Cromatografia em Camada Fina , Meios de Cultura , Eucariotos/efeitos dos fármacos , Fungos/efeitos dos fármacos , Ilex/microbiologia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Esteroides/biossíntese , Esteroides/farmacologia
16.
Nat Prod Commun ; 4(3): 395-8, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19413120

RESUMO

Three wild strains of C. acutatum have been investigated for their phytotoxic secondary metabolites involved in anthracnose disease. In addition to known compounds, a new macrolide 6 has been isolated and characterized by spectroscopic analysis.


Assuntos
Colletotrichum/química , Macrolídeos/análise , Macrolídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular
17.
Phytochemistry ; 67(2): 149-55, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16324728

RESUMO

Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC-DAD and HPLC-DAD-MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.


Assuntos
Colletotrichum/metabolismo , Hypericum/metabolismo , Fenóis/metabolismo , Acetatos/farmacologia , Células Cultivadas , Cromatografia Líquida de Alta Pressão , Colletotrichum/química , Ciclopentanos/farmacologia , Hypericum/química , Hypericum/efeitos dos fármacos , Oxilipinas , Fenóis/química , Ácido Salicílico/farmacologia , Especificidade da Espécie , Xantonas/química , Xantonas/metabolismo
18.
Plant Cell Physiol ; 46(6): 947-54, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15821288

RESUMO

Singlet oxygen is a high-energy molecular oxygen species. As one of the most active intermediates involved in chemical and biochemical reactions, singlet oxygen plays essential roles in plant responses to UV and strong light. Here, we report that Cle, an elicitor derived from fungal cell walls, induces the generation of singlet oxygen in cell cultures of ginseng, Panax ginseng. Cle treatment also triggers the activation of plasma membrane NADPH oxidase and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), subsequently leading to ethylene release and increased saponin synthesis, as shown by increased mRNA expression of squalene synthase (SQS) and squalene epoxidase (SQE), and accumulation of beta-amyrin synthase (beta-AS). Suppression of Cle-induced singlet oxygen generation or inhibition of ethylene production blocks saponin synthesis, whereas treatment of ginseng cells with ethylene or singlet oxygen induces the synthesis of saponin. Together, these results indicate that Cle-induced production of both singlet oxygen and ethylene is required for saponin synthesis, and that singlet oxygen may function upstream of ethylene during Cle-induced saponin synthesis.


Assuntos
Colletotrichum/química , Etilenos/metabolismo , Panax/metabolismo , Saponinas/biossíntese , Oxigênio Singlete/metabolismo , Colletotrichum/patogenicidade , Etilenos/farmacologia , Farnesil-Difosfato Farnesiltransferase/genética , Transferases Intramoleculares/genética , NADPH Oxidases/metabolismo , Oxigenases/genética , Panax/efeitos dos fármacos , Panax/genética , Panax/microbiologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA de Plantas/genética , RNA de Plantas/metabolismo , Oxigênio Singlete/farmacologia , Esqualeno Mono-Oxigenase
19.
Mol Microbiol ; 38(5): 940-54, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11123670

RESUMO

Colletotrichum lagenarium and Magnaporthe grisea are plant pathogenic fungi that produce melanin during the appressorial differentiation stage of conidial germination and during the late stationary phase of mycelial growth. Here, we report the identification of genes for two unique transcription factors, CMR1 (Colletotrichum melanin regulation) and PIG1 (pigment of Magnaporthe), that are involved in melanin biosynthesis. Both Cmr1p and Pig1p contain two distinct DNA-binding motifs, a Cys2His2 zinc finger motif and a Zn(II)2Cys6 binuclear cluster motif. The presence of both these motifs in a single transcriptional regulatory protein is unique among known eukaryotic transcription factors. Deletion of CMR1 in C. lagenarium caused a defect in mycelial melanization, but not in appressorial melanization. Also, cmr1Delta mutants do not express the melanin biosynthetic structural genes SCD1 and THR1 during mycelial melanization, although the expression of these two genes was not affected during appressorial melanization.


Assuntos
Colletotrichum/química , Proteínas de Ligação a DNA/química , DNA/metabolismo , Proteínas Fúngicas , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Magnaporthe/química , Melaninas/biossíntese , Transativadores/química , Transativadores/fisiologia , Fatores de Transcrição/química , Transcrição Gênica/fisiologia , Dedos de Zinco , Motivos de Aminoácidos , Sequência de Aminoácidos , Sequência de Bases , Sítios de Ligação , DNA Complementar , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/fisiologia , Regulação Fúngica da Expressão Gênica/fisiologia , Melaninas/genética , Dados de Sequência Molecular , Filogenia , Homologia de Sequência de Aminoácidos , Transativadores/genética , Fatores de Transcrição/genética , Fatores de Transcrição/fisiologia
20.
J Nat Prod ; 63(11): 1529-30, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11087599

RESUMO

A new antimicrobial metabolite, named colletotric acid (1), was isolated from a liquid culture of Colletotrichum gloeosporioides, an endophytic fungus colonized inside the stem of Artemisia mongolica. The structure was determined using spectroscopic methods (EIMS and FABMS,(1)H and (13)C NMR, (1)H-(1)H COSY, HMBC, and HMQC). Compound 1 inhibited the growth of Bacillus subtilis, Staphylococcusaureus, and Sarcina lutea with minimal inhibitory concentrations (MICs) of 25, 50, and 50 microg/mL, respectively, and the crop pathogenic fungus Helminthosporium sativum (MIC: 50 microg/mL).


Assuntos
Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Antifúngicos/isolamento & purificação , Artemisia/microbiologia , Colletotrichum/metabolismo , Plantas Medicinais , Antifúngicos/farmacologia , Artemisia/química , Bactérias/efeitos dos fármacos , Colletotrichum/química , Fermentação , Hidroxibenzoatos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Espectrometria de Massas de Bombardeamento Rápido de Átomos
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