RESUMO
Biochar could reshape microbial communities, thereby altering methylmercury (MeHg) concentrations in rice rhizosphere and seeds. However, it remains unclear whether and how biochar amendment perturbs microbe-mediated MeHg production in mercury (Hg) contaminated paddy soil. Here, we used pinecone-derived biochar and its six modified biochars to reveal the disturbance. Results showed that selenium- and chitosan-modified biochar significantly reduced MeHg concentrations in the rhizosphere by 85.83% and 63.90%, thereby decreasing MeHg contents in seeds by 86.37% and 75.50%. The two modified bicohars increased the abundance of putative Hg-resistant microorganisms Bacillus, the dominant microbe in rhizosphere. These reductions about MeHg could be facilitated by biochar sensitive microbes such as Oxalobacteraceae and Subgroup_7. Pinecone-derived biochar increased MeHg concentration in rhizosphere but unimpacted MeHg content in seeds was observed. This biochar decreased the abundance in Bacillus but enhanced in putative Hg methylator Desulfovibrio. The increasing MeHg concentration in rhizosphere could be improved by biochar sensitive microbes such as Saccharimonadales and Clostridia. Network analysis showed that Saccharimonadales and Clostridia were the most prominent keystone taxa in rhizosphere, and the three biochars manipulated abundances of the microbes related to MeHg production in rhizosphere by those biochar sensitive microbes. Therefore, selenium- and chitosan-modified biochar could reduce soil MeHg production by these microorganisms, and is helpful in controlling MeHg contamination in rice.
Assuntos
Carvão Vegetal , Quitosana , Mercúrio , Compostos de Metilmercúrio , Oryza , Selênio , Poluentes do Solo , Compostos de Metilmercúrio/análise , Poluentes do Solo/análise , Mercúrio/análise , SoloRESUMO
Cinnabaris is a traditional Chinese medicine(TCM) commonly used for sedation and tranquilization in clinics, and its safety has always been a concern. This study intends to investigate the species and tissue distribution of mercury in rats after continuous administration of Cinnabaris. In the experiment, 30 rats were randomly divided into the control group(equivalent to 0.5% carboxy-methyl cellulose sodium), low-dose Cinnabaris group(0.2 g·kg~(-1)), high-dose Cinnabaris group(2 g·kg~(-1)), pseudogerm-free control group(equivalent to 0.5% sodium carboxymethyl cellulose), and pseudogerm-free Cinnabaris group(2 g·kg~(-1)). They were orally administered for 30 consecutive days. Ultrasound-assisted acid extraction method combined with high performance liquid chromatography and inductively coupled plasma-mass spectrometry(HPLC-ICP-MS) was adopted to determine inorganic mercury [Hg(â ¡)], methylmercury(MeHg), and ethylmercury(EtHg) in different tissue, plasma, urine, and feces of rats. The optimal detection conditions and extraction methods were optimized, and the linearity(R~2>0.999 3), precision(RSD<7.0%), and accuracy(spike recoveries ranged from 73.05% to 109.5%) of all the mercury species were satisfied, meeting the requirements of analysis. The results of mercury species detection showed that Hg(â ¡) was detected in all the tissue of the five experimental groups, and the main accumulating organs were the intestinal tract, stomach, and kidney. MeHg existed at a low concentration in most tissue, and EtHg was not detected in all groups. In addition, pathological examination results showed that hepatocyte vacuolar degeneration, loose cytoplasm, light staining, and mononuclear cell infiltration were observed in the high-dose Cinnabaris group, low-dose Cinnabaris group, and pseudogerm-free Cinnabaris group, with slightly milder lesions in the low-dose Cinnabaris group. Hydrous degeneration of renal tubular epithelium could be seen in the high-dose Cinnabaris group and pseudogerm-free Cinnabaris group, but there was no significant difference between the other groups and the control group. No abnormal changes were found in the brain tissue of rats in each group. This paper studied the different mercury species and tissue distribution in normal and pseudogerm-free rats after continuous administration of Cinnabaris for 30 days and clarified its effects on the tissue structure of the liver, kidney, and brain, which provided supporting evidence for the safety evaluation of Cinnabaris.
Assuntos
Mercúrio , Compostos de Metilmercúrio , Ratos , Animais , Mercúrio/análise , Distribuição Tecidual , Compostos de Metilmercúrio/toxicidade , Compostos de Metilmercúrio/análise , Cromatografia Líquida de Alta Pressão/métodos , SódioRESUMO
In 2001 the U.S. Food and Drug Administration (FDA) issued precautionary advice to pregnant women to limit fish consumption over concern that the methylmercury content might harm their children's neurodevelopment. This concern was based largely on results from an epidemiological study of mothers primarily exposed to methylmercury from consuming pilot whale. Subsequently, FDA and the World Health Organization/Food and Agriculture Organization (WHO/FAO) undertook independent assessments of fish consumption that considered net effects from both fish nutrients, primarily omega-3 fatty acids, as beneficial and methylmercury as harmful. Both assessments estimated that when mothers regularly consume fish during pregnancy, their children are likely to have improved neurodevelopment compared to children of non-fish eaters despite their exposure to methylmercury. These estimated improvements included gains of two to over five full scale IQ points from levels of maternal consumption that are achievable in most of the world. Consistent with those estimates, human research on fish consumption and child neurodevelopment from more than 200,000 mother-child pairs now collectively reports 51 beneficial associations with neurodevelopmental outcomes and three adverse associations, the latter with no discernable pattern. These associations include full scale IQ gains similar to, or somewhat higher than, those estimated by FDA and FAO/WHO. Also consistent with the FDA and FAO/WHO estimates, research has reported beneficial associations with fish consumption when pregnant women are exposed to methylmercury from fish in excess of the U.S. Environmental Protection Agency's (EPA) Reference Dose (RfD). Our analysis evaluates how the net effects approach as utilized by FDA and FAO/WHO provides a holistic explanation for these results with implications for public health policy. This concordance of net effects modeling and empirical scientific evidence supports a clarification of current public health recommendations to focus on greater fish consumption by pregnant women for their children's neurodevelopment.
Assuntos
Ácidos Graxos Ômega-3 , Compostos de Metilmercúrio , Animais , Humanos , Feminino , Gravidez , Compostos de Metilmercúrio/toxicidade , Compostos de Metilmercúrio/análise , Alimentos Marinhos/efeitos adversos , Alimentos Marinhos/análise , Peixes , Mães , Contaminação de Alimentos/análiseRESUMO
We characterized mercury and selenium in the fish consumed in the Seychelles Islands to determine if their levels are similar to fish consumed in the US. A secondary aim was to examine whether fish weight and species predict mercury and selenium in fish consumed in the Seychelles. We measured total mercury (THg) and selenium (Se) content of 10 samples from each of the 19 most frequently consumed fish species in Seychelles and for each calculated the Se:Hg molar ratios and the Selenium Health Benefit Value Index (HBV Se). Linear regression models examined associations with weight and species. Average MeHg levels in fish ranged from less than 0.01 ppm (streamlined spinefoot) to 0.7 ppm (bludger trevally) with an overall mean of 0.21 ± 0.23 ppm. Average Se levels ranged from 0.34 ppm (blue-barred parrot fish) to 0.93 ppm (blue-lined large-eye bream) with a mean of 0.54 ± 0.23 ppm. All fish species had a mean Se:Hg molar ratio > 1 and positive mean HBV Se index values. Weight was strongly predictive of MeHg and Se:Hg molar ratio, both across and within most species, but was less predictive of Se and HBV Se. Our study demonstrated that fish consumed in Seychelles have mercury and selenium content similar to that of fish consumed in the US. Fish in both countries have favorable positive values for Se:Hg molar ratios and HBV Se indexes. Because mercury and selenium concentrations in fish are similar to those in the US but fish consumption is substantially higher in Seychelles, the Seychellois make an ideal population in which to determine if there are adverse effects of prenatal, postnatal, and lifetime low dose MeHg exposure from fish consumption.
Assuntos
Mercúrio , Compostos de Metilmercúrio , Selênio , Poluentes Químicos da Água , Animais , Mercúrio/análise , Selênio/análise , Seicheles , Poluentes Químicos da Água/análise , Peixes , Oceanos e Mares , Compostos de Metilmercúrio/análiseRESUMO
Jurujuba Cove is located in Guanabara Bay (adjacent to highly populated city of Rio de Janeiro, Brazil), which receives diffuse sources of contaminants along with two main freshwater inputs (the Cachoeira and Icaraí rivers), and hosts mussel farms. The main goal of this work was to evaluate the total mercury (THg) and methylmercury (MeHg) concentrations distributions in the sediments of the cove and their associations with physical and chemical parameters, thereby assessing their geochemical behavior. Twenty samples of surface sediments were collected and characterized for grain size, pH, redox potential, organic carbon, total phosphorus, THg and MeHg. Spatial distribution maps were produced for each parameter and a principal components analysis was carried out, to assess THg and MeHg behavior and their relationships with other parameters. The principal components analysis showed that grain size functions as the main diluting agent. The highest THg concentrations were observed in the mussel-farm area (656.1 ng g-1), and were related to fine grain size and elevated organic carbon values. High MeHg concentrations also occurred in the center of the cove, probably favored by high organic carbon content (low-energy environment). Total phosphorus concentrations indicate that Cachoeira River is a possible source of sewage, but little mercury seems to come from it. The results showed that although total mercury concentrations are elevated, with exception of a few locations, small methylmercury convertion rates were recorded in the sediments.
Assuntos
Bivalves , Mercúrio , Compostos de Metilmercúrio , Poluentes Químicos da Água , Animais , Compostos de Metilmercúrio/análise , Mercúrio/análise , Poluentes Químicos da Água/análise , Brasil , Fósforo/análise , Carbono/análise , Monitoramento Ambiental/métodos , Sedimentos Geológicos/químicaRESUMO
Methylmercury (MeHg) is a well-known environmental contaminant, particularly harmful to the developing brain. The main human dietary exposure to MeHg occurs through seafood consumption. However, seafood also contains several nutrients, including selenium, which has been shown to interact with MeHg and potentially ameliorate its toxicity. The aim of this study was to investigate the combined effects of selenium (as selenomethionine; SeMet) and MeHg on mercury accumulation in tissues and the effects concomitant dietary exposure of these compounds exert on the hippocampal proteome and transcriptome in mice. Adolescent male BALB/c mice were exposed to SeMet and two different doses of MeHg through their diet for 11 weeks. Organs, including the brain, were sampled for mercury analyses. Hippocampi were collected and analyzed using proteomics and transcriptomics followed by multi-omics bioinformatics data analysis. The dietary presence of SeMet reduced the amount of mercury in several organs, including the brain. Proteomic and RNA-seq analyses showed that both protein and RNA expression patterns were inversely regulated in mice receiving SeMet together with MeHg compared to MeHg alone. Several pathways, proteins and RNA transcripts involved in conditions such as immune responses and inflammation, oxidative stress, cell plasticity and Alzheimer's disease were affected inversely by SeMet and MeHg, indicating that SeMet can ameliorate several toxic effects of MeHg in mice.
Assuntos
Mercúrio , Compostos de Metilmercúrio , Selênio , Masculino , Adolescente , Animais , Humanos , Camundongos , Compostos de Metilmercúrio/toxicidade , Compostos de Metilmercúrio/análise , Selenometionina/farmacologia , Transcriptoma , Selênio/metabolismo , Proteoma/metabolismo , Proteômica , Camundongos Endogâmicos BALB C , Dieta , Antioxidantes , Hipocampo/metabolismo , RNARESUMO
This study measures total mercury (THg), methylmercury (MeHg) and selenium (Se) concentrations in elasmobranch fish from an Italian market with the aim of evaluating the risk-benefit associated with their consumption, using estimated weekly intake (EWI), permissible safety level (MeHgPSL), selenium health benefit value (HBVSe) and monthly consumption rate limit (CRmm) for each species. THg and Se were analysed by atomic absorption spectrometry, while MeHg was determined by HrGc/Ms. THg and MeHg concentrations ranged from 0.61 to 1.25 µg g-1 w.w. and from 0.57 to 0.97 µg g-1 w.w., respectively, whereas Se levels were 0.49-0.65 µg g-1 w.w. In most samples European Community limits for THg were surpassed, while for MeHg none of the fish had levels above the limit adopted by FAO/WHO. EWIs for THg and MeHg in many cases were above the provisional tolerable weekly intakes (PTWIs). MeHgPSL estimate showed that fish should contain approximately 50% of the concentration measured to avoid exceeding the PTWI. Nevertheless, the HBVSe index indicated that solely skates were safe for human consumption (HBVSe = 3.57-6.22). Our results highlight the importance of a constant monitoring of THg and MeHg level in fish, especially in apex predators, to avoid the risk of overexposure for consumers.
Assuntos
Elasmobrânquios , Mercúrio , Compostos de Metilmercúrio , Selênio , Poluentes Químicos da Água , Animais , Monitoramento Ambiental , Peixes , Humanos , Mercúrio/análise , Compostos de Metilmercúrio/análise , Alimentos Marinhos/análise , Selênio/análise , Poluentes Químicos da Água/análiseRESUMO
Methylmercury presents potent neurotoxicity to humans. Fish consumption is the leading source of human exposure to methylmercury worldwide. However, the exposure source in Tibet remains poorly understood because of the scarcity of observational data on most Tibetan foods, although high mercury levels were recently detected in some traditional Tibetan medicines. Here, the results of field investigations show that the joint consumption of traditional Tibetan medicines (TTMs), fish, and rice constitutes a primary exposure pathway to methylmercury in Tibetans and that the probable daily intake of methylmercury is close to that for many coastal regions. People who are young and high-income may have higher methylmercury exposure levels mainly because of economic development and cultural exchanges among regions. Our analysis indicates that a large proportion of the Tibetan population are likely to face a high methylmercury exposure risk and that mercury-susceptible populations in Tibet should be attentive to consuming TTMs with fish.
Assuntos
Mercúrio , Compostos de Metilmercúrio , Animais , Peixes , Contaminação de Alimentos/análise , Humanos , Medicina Tradicional Tibetana , Mercúrio/análise , Mercúrio/toxicidade , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/toxicidade , TibetRESUMO
An unusual mortality event (UME) attributed to morbillivirus infection was identified in two Guiana dolphin populations from the Southeastern Brazilian coast. The aim of this study was to characterize total mercury (THg), methylmercury (MeHg) and selenium (Se) bioaccumulation and body burden in Guiana dolphins from Sepetiba Bay (RJ) collected before (n = 61) and during the UME (n = 20). Significantly lower Se concentrations were found in the livers of individuals collected during the UME (Mann-Whitney test; p = 0.03), probably due to impairment of the detoxification process in the liver. There were differences in THg and Se concentrations in the organs and tissues of individuals (Kruskal-Wallis test, p < 0.05), but not MeHg (Kruskal-Wallis test, p = 0.07). For THg, the liver showed the higher concentrations and differed among organs and tissues analyzed such as blubber (Tukey's test for unequal N; p = 0.003). For Se concentrations, the skin and kidney presented the higher concentrations and varied among other tissues/organs, like muscle (Tukey's test for unequal N; p = 0.02). Differences in body burdens were observed among specimens collected previously and during the UME probably due to the remobilization and transport of the muscle-stored MeHg to other tissues/organs. This abrupt input of MeHg into the bloodstream may cause serious health damage. Indeed, evidences of methylmercury intoxication was observed in Guiana dolphins in Sepetiba Bay. In conclusion, bioaccumulation patterns, the detoxification process and body burden were affected by morbillivirus.
Assuntos
Golfinhos , Mercúrio , Compostos de Metilmercúrio , Morbillivirus , Selênio , Poluentes Químicos da Água , Animais , Carga Corporal (Radioterapia) , Brasil , Monitoramento Ambiental , Humanos , Mercúrio/análise , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/toxicidade , Selênio/análise , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Human exposure to mercury (Hg) and methylmercury (MeHg) through the ingestion of seafood raises human health-related concerns. In contrast, green tea has health benefits and its consumption potentially reduces bioaccessibility of dietary Hg. The present study aimed to assess the effect of green tea in total mercury (THg) and MeHg bioaccessibility in raw and cooked marine fish species commonly having high Hg levels. Preliminary results demonstrated that significantly higher reductions of bioaccessible THg were attained after the co-ingestion of green tea infusion (1 cup or more) in the oral and intestinal phases. Overall, the present findings clearly show that the co-ingestion of green tea along with seafood grilling strongly reduces THg and MeHg bioaccessibility in all fish species and consequently diminishes the probability of exceeding MeHg provisional tolerable weekly intakes through the consumption of these species with high Hg levels. Such results point out the need to better understand the beneficial/preventive role of green tea infusions and other food processing techniques in bioaccessibility reduction of other chemical contaminants present in food products. Such information is certainly useful to help consumers to wisely select their food, and to enable food safety authorities to integrate such information in risk assessment.
Assuntos
Camellia sinensis/química , Exposição Dietética/efeitos adversos , Peixes/metabolismo , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Chá/metabolismo , Animais , Disponibilidade Biológica , Culinária , Exposição Dietética/análise , Digestão , Peixes/classificação , Contaminação de Alimentos/análise , Humanos , Carne/análise , Mercúrio/análise , Compostos de Metilmercúrio/análise , Medição de RiscoRESUMO
BACKGROUND: We investigated Se levels along the Tapajós River basin - which is an important tributary of the Amazon River - and the possible antagonistic effect of Se in Hg availability. This is the first study to investigate Se transfer from abiotic to biotic compartments and along the food chain in aquatic ecosystems of the Amazon basin. METHODS: Se concentrations were measured in superficial sediment (nâ¯=â¯29), plankton (nâ¯=â¯28) and fishes (nâ¯=â¯121) along two stretches of the Tapajós River basin (Tapup/mi and Taplow), comprising approximately 500â¯km with different hydrological characteristics. RESULTS: Se concentrations in sediment were significantly higher in the Taplow (345-664⯵g kg-1) than in the Tapup/mi (60-424⯵g kg-1). The seasonal flooding of the Amazon River probably helps to carry selenium-rich sediment to the Tapajós mouth (Taplow stretch). We suggest that Se in sediment could decrease the bioavailability of Hg resulting in lower MeHg concentrations in fish, as observed in the Taplow (45-934⯵g kg-1). Sediment and plankton were positively correlated in relation to their Se concentrations (râ¯=â¯0.62; pâ¯=â¯0.001) suggesting that sediment can possibly be the main source of Se to plankton. Our data indicate Se uptake by primary consumers, as noted in phytoplankton levels. The decrease of Se concentrations along the food chain was also noteworthy. CONCLUSION: This work elucidates some aspects of Se biogeochemistry in the Amazon basin and shows its importance regarding Hg cycles in aquatic ecosystems.
Assuntos
Mercúrio/análise , Compostos de Metilmercúrio/análise , Selênio/análise , Animais , Ecossistema , Monitoramento Ambiental , Peixes , Rios/químicaRESUMO
Traditional foods provide nutritional, social, and economic benefits for Indigenous communities; however, anthropogenic activities have raised concerns about mercury (Hg), especially methylmercury (MeHg), in these foods. This issue may be of particular concern for communities near large industrial activities, including the Bigstone Cree Nation adjacent to the Athabasca oil sands region, Canada. This community-led study sought to assess variation in THg and MeHg concentrations among traditional food types (plants or animals), species, and tissues (muscles, organs), and variation in concentrations of the micronutrient selenium (Se)- thought to protect against Hg toxicity-and Se:THg ratios. Thirteen plant and animal species were collected in 2015 by Bigstone Cree community members. We quantified THg, Se, and Se:THg ratios in 65 plant and 111 animal samples and MeHg in 106 animal samples. For plants, the lichen, old man's beard (Usnea spp.), showed the highest concentrations of THg and Se (0.11 ± 0.02 and 0.08 ± 0.01 µg g-1 w. w., respectively) and also had a low Se:THg molar ratio. Concentrations of THg, MeHg, and Se differed among animal samples (P < 0.010), showing variation among species and among tissues/organs. Generally, concentrations of THg and MeHg were highest in aquatic animals, which also had relatively low Se:THg molar ratios. Overall results revealed substantial variation in the patterns of THg, MeHg, Se and Se:THg ratios across this comprehensive basket of traditional foods. Thus, measuring concentrations of THg alone, without considering MeHg and potential associations with Se, may not adequately convey the exposure to Hg in traditional foods.
Assuntos
Monitoramento Ambiental/métodos , Análise de Alimentos/métodos , Mercúrio/análise , Compostos de Metilmercúrio/análise , Selênio/análise , Alberta , Animais , Cadeia Alimentar , Humanos , Poluentes Químicos da Água/análiseRESUMO
BACKGROUND: Health risks linked with dioxin in fish remain a complex policy issue. Fatty Baltic fish contain persistent pollutants, but they are otherwise healthy food. We studied the health benefits and risks associated with Baltic herring and salmon in four countries to identify critical uncertainties and to facilitate an evidence-based discussion. METHODS: We performed an online survey investigating consumers' fish consumption and its motivation in Denmark, Estonia, Finland, and Sweden. Dioxin and methylmercury concentrations were estimated based on Finnish studies. Exposure-response functions for several health endpoints were evaluated and quantified based on the scientific literature. We also quantified the infertility risk of men based on a recent European risk assessment estimating childhood dioxin exposure and its effect on sperm concentration later in life. RESULTS: Baltic herring and salmon contain omega-3 fatty acids and vitamin D, and the beneficial impact of these fishes on cardiovascular diseases, mortality, and the risk of depression and cancer clearly outweighs risks of dioxins and methylmercury in people older than 45 years of age and in young men. Young women may expose their children to pollutants during pregnancy and breast feeding. This study suggests that even in this critical subgroup, the risks are small and the health benefits are greater than or at least similar to the health risks. Value of information analysis demonstrated that the remaining scientific uncertainties are not large. In contrast, there are several critical uncertainties that are inherently value judgements, such as whether exceeding the tolerable weekly intake is an adverse outcome as such; and whether or not subgroup-specific restrictions are problematic. CONCLUSIONS: The potential health risks attributable to dioxins in Baltic fish have more than halved in the past 10 years. The new risk assessment issued by the European Food Safety Authority clearly increases the fraction of the population exceeding the tolerable dioxin intake, but nonetheless, quantitative estimates of net health impacts change only marginally. Increased use of small herring (which have less pollutants) is a no-regret option. A more relevant value-based policy discussion rather than research is needed to clarify official recommendations related to dioxins in fish.
Assuntos
Poluentes Ambientais/efeitos adversos , Poluentes Ambientais/análise , Peixes , Contaminação de Alimentos/análise , Alimentos Marinhos/análise , Adulto , Animais , Criança , Dioxinas/efeitos adversos , Dioxinas/análise , Estudos de Avaliação como Assunto , Feminino , Humanos , Recém-Nascido , Infertilidade Masculina/induzido quimicamente , Masculino , Compostos de Metilmercúrio/efeitos adversos , Compostos de Metilmercúrio/análise , Valor Nutritivo , Gravidez , Medição de Risco , Salmão , Países Escandinavos e Nórdicos , Doenças Dentárias/induzido quimicamenteRESUMO
Water-level-fluctuating zone (WLFZ) is a prevalent water-land ecotone favorable for mercury (Hg) methylation. The succession of flooding tolerance plants in WLFZ gradually changes the landscape, and also brings a new question worth understanding whether these plants would enhance methylmercury (MeHg) production in WLFZ and increase risks to the aquatic environment. Given bermudagrass (Cynodon dactylon (L). Pers) as the dominant perennial herb with high flooding-tolerance in WLFZ of the Three Gorges Reservoir (TGR), we conducted a comprehensive study to investigate its roles in the production and migration of MeHg in WLFZ by field observations and stable isotope tracer experiments. Results showed that both elevated MeHg levels and Hg methylation rates appeared in soil/sediment in bermudagrass growing area, implying that the growth of bermudagrass could significantly enhance MeHg production. However, MeHg migration from sediment to water was restricted during the flooding period of the TGR, as obviously higher partitioning coefficients of MeHg between the sediment and porewater (pâ¯<⯠0.05) and lower MeHg release fluxes were observed in vegetated area, indicating that the presence of bermudagrass instead probably decreased the water MeHg level. Whereas, it is noteworthy that elevated MeHg in soil/sediment induced by the bermudagrass could pose potential risks to the benthos and further to the TGR food chain.
Assuntos
Cynodon/metabolismo , Mercúrio/metabolismo , Compostos de Metilmercúrio , Poluentes Químicos da Água , China , Sedimentos Geológicos/análise , Metilação , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/metabolismo , Solo/química , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo , Abastecimento de ÁguaRESUMO
Methylmercury (MeHg) in rice grains grown in Hg-contaminated areas has raised environmental health concerns. Pot experiments found that selenium (Se) could reduce MeHg levels in rice grains. However, relatively high levels of Se (up to 6â¯mg/kg) were applied in these pot experiments, which may have adverse effects on the soil ecology due to the toxicity of Se. The aims of this work were thus to study 1) the effect of low levels of Se on the accumulation and distribution of Hg, especially MeHg, in rice plants grown in a real Hg-contaminated paddy field and 2) the effect of Se treatment on Se and other nutritional elements (e.g., Cu, Fe, Zn) in grains. A field study amended with different levels of Se was carried out in Hg-contaminated paddy soil in Qingzhen, Guizhou, China. The levels of MeHg and total Hg were studied using cold vapor atomic fluorescence spectrometry (CVAFS) and inductively coupled plasma mass spectrometry (ICP-MS). The distribution and relative quantification of elements in grains were examined by synchrotron radiation X-ray fluorescence analysis (SR-XRF). This field study showed that low levels of Se (0.5⯵g/mL, corresponding to 0.15â¯mg Se/kg soils) could significantly reduce total Hg and MeHg in rice tissues. Se treatment also reduced Hg distribution in the embryo and endosperm and increased the levels of Fe, Cu, Zn and Se in grains and especially embryos. This field study implied that treatment with an appropriate level of Se is an effective approach to not only decrease the level of MeHg but to also increase the levels of nutritional elements such as Fe, Cu, Zn and Se in rice grains, which could bring beneficial effects for rice-dependent residents living in Hg-contaminated areas.
Assuntos
Mercúrio/análise , Compostos de Metilmercúrio/análise , Oryza/efeitos dos fármacos , Selênio/farmacologia , Poluentes do Solo/análise , China , Fazendas , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Oryza/crescimento & desenvolvimento , Oryza/metabolismo , Solo/química , Poluentes do Solo/metabolismoRESUMO
Mercury still represents one of the most hazardous threats for the aquatic ecosystem due to its high toxicity, and the fact that it can be easily incorporated into the food chain by accumulation in fish as MeHg. On the other hand, selenium is a micronutrient that is part of different antioxidant enzymes that regulate the cellular redox state, and whose complex interaction with Hg has been extensively studied from a toxicological point of view. In order to evaluate the protective effect of Se(IV) co-administration against MeHg accumulation and toxicity, we have selected an in-vivo model at two developmental stages: zebrafish eleutheroembryos and adult fish. Embryos were exposed during 48â¯h to MeHg (5 or 25⯵g/l) and a concentration of Se (IV) representing a molar ratio close to one (2.5 or 12.5⯵g/l), while adult zebrafish were exposed during 72â¯h to either 25⯵g/l of MeHg alone or co-exposed with 12.5⯵g/l of Se (IV). A significant decrease in MeHg bioaccumulation factor was observed in eleutheroembryos co-exposed to Se(IV). A time-dependent accumulation of MeHg was observed in all the analyzed organs and tissues of adult fish, which was significantly reduced in the muscular tissue and the intestine by Se(IV) co-administration. However, such protection against MeHg bioaccumulation was not maintained in the brain and liver. The data derived from the gene expression analysis also demonstrated the protective effect of Se(IV) against MeHg-induced oxidative stress and the activation of different defense mechanisms by Se(IV) co-administration.
Assuntos
Mercúrio/análise , Compostos de Metilmercúrio/análise , Selênio/farmacologia , Poluentes Químicos da Água/análise , Peixe-Zebra/metabolismo , Animais , Antioxidantes/metabolismo , Química Encefálica/efeitos dos fármacos , Ecossistema , Cadeia Alimentar , Expressão Gênica/efeitos dos fármacos , Fígado/química , Fígado/metabolismo , Músculos/química , Peixe-Zebra/embriologiaRESUMO
OBJECTIVE: Long-chain omega-3 polyunsaturated fatty acids (PUFA) from fish have been inversely associated with coronary heart disease (CHD) risk. Fish may also contain methylmercury, which has been associated with higher CHD risk and may diminish the cardioprotective effect of long-chain omega-3 PUFA. We investigated the associations of serum long-chain omega-3 PUFA and hair mercury with the odds for myocardial ischaemia during exercise. METHODS: A total of 2199 men from the Kuopio Ischaemic Heart Disease Risk Factor Study, aged 42-60 years were studied in 1984-89. Of the 2199 men, 342 had history of CHD. The men performed a maximal symptom-limited exercise stress test using an electrically braked bicycle ergometer. ORs for exercise-induced myocardial ischaemia were estimated with logistic regression. RESULTS: In the multivariable analysis, those in the highest versus lowest serum long-chain omega-3 PUFA quartile had 33% lower odds of myocardial ischaemia (OR 0.67, 95% CI 0.51 to 0.87, p-trend=0.006). The association was stronger among those with CHD history (OR 0.10, 95% CI 0.03 to 0.39, p-trend <0.001), than among those without (OR 0.80, 95% CI 0.57 to 1.12, p-trend=0.17) (p-interaction=0.01). Higher hair mercury concentration was associated with increased odds for myocardial ischaemia in the entire population (OR 1.62, 95% CI 1.22 to 2.14, p-trend=0.002). CONCLUSION: Higher circulating concentrations of the long-chain omega-3 PUFAs, a marker for fish consumption, were associated with lower occurrence of exercise-induced myocardial ischaemia, but only among men with CHD history. Hair mercury concentration was directly associated with the occurrence of exercise-induced myocardial ischaemia in the entire study population.
Assuntos
Dieta Saudável , Exercício Físico , Ácidos Graxos Ômega-3/sangue , Cabelo/química , Compostos de Metilmercúrio/análise , Isquemia Miocárdica/metabolismo , Alimentos Marinhos , Adulto , Fatores Etários , Biomarcadores/sangue , Carga Corporal (Radioterapia) , Estudos Transversais , Finlândia , Humanos , Masculino , Compostos de Metilmercúrio/efeitos adversos , Pessoa de Meia-Idade , Isquemia Miocárdica/sangue , Isquemia Miocárdica/diagnóstico , Isquemia Miocárdica/epidemiologia , Medição de Risco , Fatores de Risco , Fatores SexuaisRESUMO
Rice grain is known to accumulate methylmercury (MeHg) and has been confirmed to be the major pathway of MeHg exposure to residents in mercury (Hg) mining areas in China. Selenium (Se) supplementation has been proven to be effective in mitigating the toxicity of Hg. To understand how Se supplementation influences soil Hg speciation, a wide range of Se (0-500â¯mg/kg) was applied to Hg polluted paddy soils in this study, which decreased MeHg concentration in soil from 2.95⯱â¯0.36 to 0.69⯱â¯0.16⯵g/kg (or 77%). After Se addition, humic acid state Hg (F4) was transformed into strong-complexed state Hg (F5), indicating that Hg bound up to the non-sulfur functional groups of humic acid (non-RSH) was released and reabsorbed by strong binding Se functional group (F5). As a result, inorganic Hg (IHg) was reduced by >48%, 18%, and 80% in root, stem, and grain, respectively, however, the reduction was not apparent in leaf. Substantial reductions were also found for MeHg in grain and root, but not in stem and leaf. Soil is suggested to be the main source of both MeHg and IHg in rice grain. Such a finding may provide an idea for improving Hg-polluted paddies through controlling soil IHg and MeHg. Further research on the molecular structure of the strong-complexed Hg in F5 should be conducted to elucidate the mechanism of Hg-Se antagonism.
Assuntos
Compostos de Mercúrio/análise , Mercúrio/análise , Compostos de Metilmercúrio/análise , Oryza/efeitos dos fármacos , Selênio/química , Poluentes do Solo/análise , Transporte Biológico , China , Grão Comestível/química , Substâncias Húmicas/análise , Compostos de Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Mineração , Oryza/metabolismo , Estruturas Vegetais/metabolismo , Selênio/farmacologia , Solo/químicaRESUMO
Evaluating potential ecological and human health risks of exposure to bioaccumulative trace elements is typically implemented using analysis of tissue samples. Increasingly, the microchemistry of fish calcified structures is used to elucidate the lifetime exposure to trace elements. In the present study, we measured total mercury (THg), methylmercury (MeHg), and selenium (Se) in muscle tissue and otolith samples from 12 species of fish collected at reference sites and locations influenced by power plant wastewater. Muscle tissue concentrations of Se were sensitive to recent wastewater exposure magnitude, stream type, trophic level, and species (p < 0.001). For Hg, concentrations in muscle tissue and otoliths were affected only by trophic level and species. Levels of THg and Se in muscle tissue and otolith samples were positively correlated for those species with a robust sample size. Some individual fish from 3 species (channel catfish, hybrid striped bass, and freshwater drum) showed significantly increasing or decreasing lifetime concentrations of either THg or Se in otolith samples. Multiple regression analysis indicated that for bluegill muscle tissue Se concentrations could be best explained utilizing water concentrations of selenium, sulfate, and molybdenum (r2 = 0.87; p < 0.001). Because of the increased cost and specialized sample processing requirements of analyzing trace elements in otolith structures, it may be prudent to limit these analyses to those species where insights into temporal trends are sought or where evidence indicates that fish move into or out of contaminated water bodies. Environ Toxicol Chem 2019;38:1467-1475. © 2019 SETAC.
Assuntos
Mercúrio/análise , Músculos/química , Membrana dos Otólitos/química , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Monitoramento Ambiental , Peixes , Água Doce/análise , Humanos , Modelos Lineares , Espectrometria de Massas , Compostos de Metilmercúrio/análise , Músculos/metabolismo , Membrana dos Otólitos/metabolismo , Poluentes Químicos da Água/químicaRESUMO
Methylmercury (MeHg) bioaccumulation in freshwater aquatic systems is impacted by anthropogenic stressors, including climate change and nutrient enrichment. The goal of this study was to determine how warmer water temperatures and excess nutrients would alter zooplankton communities and phytoplankton concentrations, and whether those changes would in turn increase or decrease MeHg concentrations in freshwater zooplankton. To test this, we employed a 2â¯×â¯2 factorial experimental design with nutrient and temperature treatments. Mesocosms were filled with ambient water and plankton from Cottage Grove Reservoir, Oregon, U.S.A., a waterbody that has experienced decades of elevated MeHg concentrations and corresponding fish consumption advisories due to run-off from Black Butte Mine tailings, located within the watershed. Treatment combinations of warmer temperature (increased by 0.7⯰C), nutrient addition (a single pulse of 10× ambient concentrations of nitrogen and phosphorous), control, and a combination of temperature and nutrients were applied to mesocosms. The individual treatments altered phytoplankton densities and community structure, but alone the effects on MeHg concentrations were muted. Importantly, we found a significant interactive effect of nutrients and temperature: the nutrient addition appeared to buffer against increased MeHg concentrations associated with elevated temperature. However, there was variability in this response, which seems to be related to the abundance of Daphnia and edible phytoplankton. Nutrients at low temperature were associated with marginal increases (1.1×) in zooplankton MeHg. Our findings suggest that global change drivers that influence community composition and ecosystem energetics of both zooplankton and phytoplankton can alter MeHg pathways through food webs.