RESUMO
Despite the versatility of quantum dots (QDs) in optoelectronics and biomedical field, their toxicity risks remain a considerable hindrance for clinical applications. Cytotoxicity of Cadmium containing QDs is well documented and reveals that they are toxic to cells. Reports suggest that the presence of toxic elements at the QD core (e.g., cadmium, selenium) is responsible for its toxicity in in vivo and in vitro levels. Hence, here the toxicity of heavy metal free ZnSe/ZnS QDs on two scenarios were assessed, (i) HEK cells as in vitro system and (ii) Swiss Albino mice as in vivo model. Before toxicity analysis, QDs subjected to various optical and physico-chemical characterization methods such as absorption and emission spectra analysis, observation under U.V light, TEM, DLS, Zeta potential, FTIR, Raman and XPS spectra, ICP-OES, TGA and DTG curve. It is very necessary to characterize the synthesized QDs because their toxicity greatly influenced by the physico-chemical properties. On checking the vulnerability of HEK cells on exposure to ZnSe/ZnS QDs, the obtained results disclose that ZnSe/ZnS QDs showed merest impact on cellular viability at a concentration less than 100 µg/ml. Acute toxicity of 10 mg/kg ZnSe/ZnS QDs was studied in mice and no clinical or behavioural changes were observed. It did not induce any changes in haematological parameters and any loss of body or organ weight. Moderate pathological changes were evident only in the liver, all others organs like kidney, spleen and brain did not show any manifestations of toxicity. Current work lays substantial bedrock for safe biomedical and environmental application of ZnSe/ZnS QDs in near future.
Assuntos
Pontos Quânticos/toxicidade , Selênio/toxicidade , Sulfetos/toxicidade , Compostos de Zinco/toxicidade , Zinco/toxicidade , Animais , Peso Corporal/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Endocitose/efeitos dos fármacos , Células HEK293 , Humanos , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Camundongos , Tamanho do Órgão/efeitos dos fármacos , Pontos Quânticos/análise , Selênio/análise , Baço/efeitos dos fármacos , Baço/patologia , Sulfetos/análise , Testes de Toxicidade , Zinco/análise , Compostos de Zinco/análiseRESUMO
In archaeology, the discovery of ancient medicines is very rare, as is knowledge of their chemical composition. In this paper we present results combining chemical, mineralogical, and botanical investigations on the well-preserved contents of a tin pyxis discovered onboard the Pozzino shipwreck (second century B.C.). The contents consist of six flat, gray, discoid tablets that represent direct evidence of an ancient medicinal preparation. The data revealed extraordinary information on the composition of the tablets and on their possible therapeutic use. Hydrozincite and smithsonite were by far the most abundant ingredients of the Pozzino tablets, along with starch, animal and plant lipids, and pine resin. The composition and the form of the Pozzino tablets seem to indicate that they were used for ophthalmic purposes: the Latin name collyrium (eyewash) comes from the Greek name κoλλυρα, which means "small round loaves." This study provided valuable information on ancient medical and pharmaceutical practices and on the development of pharmacology and medicine over the centuries. In addition, given the current focus on natural compounds, our data could lead to new investigations and research for therapeutic care.
Assuntos
Medicina Tradicional/história , Arqueologia , Etnobotânica , História Antiga , Humanos , Itália , Preparações de Plantas/química , Preparações de Plantas/história , Plantas Medicinais/química , Pólen , Navios/história , Espectroscopia de Infravermelho com Transformada de Fourier , Comprimidos/química , Comprimidos/história , Compostos de Zinco/análiseRESUMO
The present mechanistic in vitro study aimed to investigate dose-response effects of zinc and fluoride on caries lesion remineralization and subsequent protection from demineralization. Artificial caries lesions were created using a methylcellulose acid gel system. Lesions were remineralized for 2 weeks using citrate-containing artificial saliva which was supplemented with zinc (0-153 µmol/l) and fluoride (1.1 or 52.6 µmol/l) in a 7 × 2 factorial design. Lesions were also remineralized in the absence of zinc and citrate, but in the presence of fluoride. After remineralization, all lesions were demineralized for 1 day under identical conditions. Changes in mineral distribution characteristics of caries lesions after remineralization and secondary demineralization were studied using transverse microradiography. At 1.1 µmol/l fluoride, zinc exhibited detrimental effects on remineralization in a dose-response manner and mainly by preventing remineralization near the lesion surface. At 52.6 µmol/l fluoride, zinc retarded remineralization only at the highest concentration tested. Zinc enhanced overall remineralization at 3.8-15.3 µmol/l. At 76.5 and less so at 153 µmol/l, zinc showed extensive remineralization of deeper parts within the lesions at the expense of remineralization near the surface. Citrate did not interfere with remineralization at 1.1 µmol/l fluoride, but enhanced remineralization at 52.6 µmol/l fluoride. Lesions exhibiting preferential remineralization in deeper parts showed higher mineral loss after secondary demineralization, suggesting the formation of more soluble mineral phases during remineralization. In summary, zinc and fluoride showed synergistic effects in enhancing lesion remineralization, however only at elevated fluoride concentrations.
Assuntos
Cariostáticos/administração & dosagem , Cárie Dentária/fisiopatologia , Esmalte Dentário/efeitos dos fármacos , Fluoretos/administração & dosagem , Remineralização Dentária/métodos , Zinco/administração & dosagem , Animais , Apatitas/análise , Fluoreto de Cálcio/análise , Fosfatos de Cálcio/análise , Bovinos , Ácido Cítrico/farmacologia , Cárie Dentária/patologia , Esmalte Dentário/patologia , Solubilidade do Esmalte Dentário/efeitos dos fármacos , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Durapatita/análise , Concentração de Íons de Hidrogênio , Ácido Láctico/efeitos adversos , Metilcelulose/química , Microrradiografia , Minerais/análise , Fosfatos/análise , Saliva Artificial/química , Compostos de Zinco/análiseRESUMO
Based on the phase and composition analysis of 56 batches of samples, the present paper showed that hydrozincite, just as smithsonite, should be named as the mineralogical origin of medicinal galamina. Galamina was proved to be polymineral aggregation by electron probe micro-analysis, which was constituted by various mineral particulates, such as hydrozincite, smithsonite, zinc oxide, dolomite, etc. It is hydrozincite but not smithsonite that is the current mainstream mineral of commercial galamina. Both hydrozincite and smithsonite should be calcined to turn into zinc oxide when they were used as medicine. As a provider of medical galamina, essentially the zinc oxide, hydrozincite is appropriate.
Assuntos
Minerais/análise , Preparações Farmacêuticas/química , Compostos de Zinco/análise , Medicina Tradicional ChinesaRESUMO
Citrate complexes of Mn and Fe, and potentially those of Cu and Zn, are considered as important low molecular mass species in human serum and cerebrospinal fluid (CSF). For example, Mn is supposed to enter the brain under excess exposure as Mn-citrate leading to neurotoxic effects. Mn-citrate has been characterised in human CSF using chromatography and electrophoresis online with inductively coupled plasma mass spectrometry, but not yet with molecular mass spectrometry. Therefore, this study explores the potential of electrospray ionisation (ESI) with selected reaction monitoring (SRM) for the detection of metal-citrate complexes, in particular Mn-citrate. The collision-induced dissociation of precursor ions with various metal:citrate stoichiometries was studied for Mn-citrate, Fe-citrate, Cu-citrate and Zn-citrate. High selectivity was achieved for Mn(II)-citrate even in respect to Fe(III)-citrate which forms isobaric precursor ions. The limit of detection for Mn-citrate was estimated to be around 250 microg L(-1) (referring to the total Mn content in the standard) using flow injection. The sensitivity was sufficient for the determination of Mn-citrate in standard solutions and in an extract of an Mn-citrate-containing supplement. An improved ESI source design is expected to reduce the limits of detection significantly. The developed ESI-SRM method has the potential to provide complementary data for the quality control of current separation methods for metal citrates using element-selective detection, with application to biomedical samples and further matrices.
Assuntos
Citratos/análise , Cobre/análise , Compostos de Ferro/análise , Compostos de Manganês/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Compostos de Zinco/análise , Citratos/química , Cobre/química , Suplementos Nutricionais/análise , Humanos , Compostos de Ferro/química , Compostos de Manganês/química , Sensibilidade e Especificidade , Compostos de Zinco/químicaRESUMO
The applicability of spectrochemical analysis of minute amounts of powder samples was investigated using an ultraviolet Nd-YAG laser (355 nm) and low-pressure ambient air. A large variety of chemical powder samples of different composition were employed in the experiment. These included a mixture of copper(II) sulfate pentahydrate, zinc sulfide, and chromium(III) sulfate n-hydrate powders, baby powder, cosmetic powders, gold films, zinc supplement tablet, and muds and soils from different areas. The powder samples were prepared by pulverizing the original samples to an average size of around 30 microm in order to trap them in the tiny micro holes created on the surface of the quartz subtarget. It was demonstrated that in all cases studied, good quality spectra were obtained with low background, free from undesirable contamination by the subtarget elements and featuring ppm sensitivity. A further measurement revealed a linear calibration curve with zero intercept. These results clearly show the potential application of this technique for practical qualitative and quantitative spectrochemical analysis of powder samples in various fields of study and investigation.
Assuntos
Lasers de Estado Sólido , Pós/análise , Análise Espectral/métodos , Ar , Ouro/análise , Compostos Organometálicos/análise , Tamanho da Partícula , Pressão , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Solo/análise , Análise Espectral/instrumentação , Sulfetos/análise , Compostos de Zinco/análiseRESUMO
Seven zinc salts--acetate, chloride, lactate, sulfate, citrate, gluconate, and oxide--were added to milk--and soy-based infant formulas to estimate possible differences in zinc availability depending on the type of salt used. For this purpose, an in vitro method that estimates the dialyzability of the element (i.e., the fraction available for absorption) was applied. Zinc dialyzability is always higher in milk-based products than in soy products, even when the total zinc contents are higher in the latter. The salts can be classified according to the zinc dialyzability in the two types of formulas as follows: oxide > gluconate = chloride = lactate > citrate = acetate > sulfate. Therefore, according to the dialysis percentage, oxide and gluconate are the compounds of choice for zinc supplementation of infant formulas.
Assuntos
Alimentos Infantis/análise , Compostos de Zinco/análise , Zinco/química , Animais , Disponibilidade Biológica , Diálise , Humanos , Indicadores e Reagentes , Lactente , Leite/química , Glycine max/química , Espectrofotometria AtômicaRESUMO
The effect of zinc on protein synthesis in the femoral-metaphyseal tissues of normal and skeletally unloaded rats was investigated. Skeletal unloading was designed using the model of hindlimb suspension in rats. Animals were fed for 2 or 4 days during the unloading. [3H]Leucine was added to the reaction mixture containing the 5500 g supernatant fraction of the homogenate prepared from the femoral-metaphyseal tissues. In vitro protein synthesis was significantly decreased in the bone tissues from the rats which had undergone unloading for 2 or 4 days. When the metaphyseal tissues were cultured for 24 h in the presence of zinc sulfate (10(-5) M) or beta-alanyl-L-histidinato zinc (AHZ, 10(-5) M), zinc compounds clearly stimulated protein synthesis in the metaphyseal tissues from the 4-day unloaded rats. The zinc effect was also seen in the metaphyseal tissues from normal rats. The addition of zinc sulfate (10(-5) M) or AHZ (10(-7) to 10(-5) M) into the reaction mixture containing the 5500 g supernatant fraction of metaphyseal homogenate from normal or unloaded rats produced a significant increase in protein synthesis. This increase was clearly inhibited in the presence of cycloheximide (10(-7) M). The present result demonstrates that protein synthesis is impaired in the femoral-metaphyseal tissues of rats with skeletal unloading, and that this impairment is clearly restored by zinc supplementation.
Assuntos
Doenças Ósseas Metabólicas/metabolismo , Fêmur/metabolismo , Biossíntese de Proteínas , Compostos de Zinco/farmacologia , Zinco/metabolismo , Animais , Antiulcerosos/farmacologia , Desenvolvimento Ósseo , Doenças Ósseas Metabólicas/etiologia , Carnosina/análogos & derivados , Carnosina/farmacologia , Células Cultivadas , Técnicas de Cultura , Cicloeximida/farmacologia , Diáfises/metabolismo , Feminino , Fêmur/citologia , Fêmur/efeitos dos fármacos , Elevação dos Membros Posteriores/efeitos adversos , Compostos Organometálicos/farmacologia , Inibidores da Síntese de Proteínas/farmacologia , Ratos , Ratos Wistar , Compostos de Zinco/análiseRESUMO
A pot-culture experiment was conducted to assess the bioavailability of iron from spinach cultivated in soil fortified with graded levels of iron and zinc (FeSO4 x 7H2(0) and ZnSO4 x 7H2(0), respectively). Applications of varying levels of iron to soil increased the total iron and phosphorus contents and decreased the zinc content (P < 0.05). The effect of applying varying levels of zinc was the opposite of on the minerals in spinach. The ascorbic acid content was remarkably reduced with varying levels of iron and zinc. Higher levels of zinc and lower levels of iron in the soil increased the bioavailability of iron from spinach (P < 0.05). In conclusion, the interactions of 15 ppm zinc with 30 ppm iron significantly enhanced the bioavailability of iron, total iron and zinc contents.