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1.
Food Chem ; 362: 130224, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34098439

RESUMO

This study evaluated the feasibility of curcumin based photodynamic sterilization technology (PDT) applied to fresh-cut potato slices. Potato samples with 30 µmol L-1 curcumin solution were exposed to 420 nm light emitting diodes (LED) at a total dose of 0.7 kJ cm-2. Results showed that PDT inactivated 2.43 log CFU mL-1 of Escherichia coli (BL 21) and 3.18 log CFU mL-1 of Staphylococcus aureus and maintained the color, texture, weight as well as total solid content of treated potatoes. Additionally, loss of phenols and flavonoids was significantly prevented, increasing the total antioxidant capacity. This was attributed to changes in enzyme activity that PDT decreased the activity of polyphenol oxidase (PPO) and peroxidase (POD) by 59.7% and 47.8% and increased the activity of phenylalanine ammonia-lyase (PAL). Therefore, curcumin-based PDT has the potential to maintain the commercial quality of producing and achieving microbiological safety.


Assuntos
Antioxidantes/química , Curcumina/farmacologia , Conservação de Alimentos/métodos , Solanum tuberosum/química , Solanum tuberosum/microbiologia , Antibacterianos/farmacologia , Catecol Oxidase/metabolismo , Cor , Escherichia coli , Flavonoides/análise , Flavonoides/química , Conservação de Alimentos/instrumentação , Qualidade dos Alimentos , Peroxidase/química , Peroxidase/metabolismo , Fenóis/análise , Fenóis/química , Fenilalanina Amônia-Liase/metabolismo , Solanum tuberosum/efeitos dos fármacos , Solanum tuberosum/metabolismo , Staphylococcus aureus
2.
Food Chem ; 361: 129832, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34023688

RESUMO

Resveratrol-loaded fish gelatin (FG)-low methoxyl pectin (LMP) composite films with different FG:LMP mass ratios were prepared and evaluated as food packaging materials. With increasing FG contents, the water solubility of the films decreased. Moreover, the UV (315-400 nm) blocking efficiency and opacity increased with increasing LMP contents. The elongation of the films at breaking and tensile strengths were adjusted using the ratio of FG and LMP. The lowest water vapour permeability was observed at an FG:LMP mass ratio of 2:1. All films exhibited good antioxidant properties and significantly delayed oil deterioration when used for beef tallow preservation. The release behaviour of resveratrol in 95% ethanol as a food simulant was determined by film composition. The fabricated films exhibit significant potential for beef tallow preservation applications. Furthermore, LMP can improve the stability of resveratrol-FG complexes and compete with resveratrol for binding FG to accelerate resveratrol release.


Assuntos
Embalagem de Alimentos , Gelatina/química , Pectinas/química , Resveratrol/química , Animais , Antioxidantes/química , Produtos Pesqueiros , Conservação de Alimentos/instrumentação , Armazenamento de Alimentos , Concentração de Íons de Hidrogênio , Permeabilidade , Carne Vermelha , Resveratrol/farmacocinética , Solubilidade , Vapor , Resistência à Tração
3.
J Sci Food Agric ; 101(14): 5775-5783, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33788966

RESUMO

BACKGROUND: This study reports the use of low glycemic sunflower pectin gel, elaborated with calcium and without or with sweeteners (sucrose, stevia and saccharin) as an edible coating and its possible combination with two modified atmosphere packaging (MAP), in order to extend shelf life, maintaining the quality, of strawberries during the storage at 4 °C. RESULTS: This pectin coating, formed with only calcium and/or stevia or saccharin, extended the shelf life of strawberries with respect to uncoating fruits, up to 12 days, keeping the microbial load constant, the firmness and less weight loss. With the same edible coatings, the shelf life of strawberries was extended up to 23 days when they were combined with MAP [10% carbon dioxide (CO2 ), 85% nitrogen (N2 ) and 5% oxygen (O2 )], maintaining the quality of strawberries, while the other MAP, with a higher CO2 concentration (20% CO2 , 75% N2 and 5% O2 ), had no effect. CONCLUSIONS: These results highlight the suitability of the combination of edible pectin coating combined with MAP to obtain an important shelf life extension, maintaining the good quality of the fruit. © 2021 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Fragaria/química , Frutas/química , Helianthus/química , Pectinas/química , Extratos Vegetais/química , Filmes Comestíveis , Conservação de Alimentos/instrumentação , Armazenamento de Alimentos , Índice Glicêmico
4.
J Sci Food Agric ; 101(10): 4229-4240, 2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-33426638

RESUMO

BACKGROUND: In the postharvest handling of horticultural commodities, plant extracts with fungicidal activity are a valid alternative to synthetic fungicides. The fungicidal activity of myrtle leaf extracts from eight cultivars was studied in vitro against Penicillium digitatum, Penicillium italicum, and Penicillium expansum and on artificially inoculated mandarins with green and blue molds during storage for 12 days at 20 °C and 90% RH. RESULTS: Hydroxybenzoic acids, hydrolysable tannins, and flavonols were identified by high-performance liquid chromatography (HPLC). Despite sharing the same phenolic profile, extracts of eight myrtle cultivars significantly differed in the concentration of phenolics. Hydrolysable tannins are the principal subclass representing nearly 44.9% of the total polyphenols, whereas myricitrin was the most abundant flavonol in all cultivars. Myrtle extracts strongly inhibited conidial germination of the pathogens tested, although the greatest efficacy was observed against P. digitatum. At a concentration of 20 g L-1 , all the extracts completely inhibited fungi growth; only 'Angela', 'Tonina' and 'Grazia' extracts were effective at lower concentrations (15 g L-1 ). On inoculated fruit, myrtle extracts significantly controlled rot development. As a preventive treatment, 'Ilaria' and 'Maria Rita' extracts significantly reduced the rate of fruit with green mold decay lesions. When applied as a curative treatment, all the exacts decreased the incidence of decay. Against P. italicum, all the extracts applied as preventive treatments controlled decay effectively, while as curative treatment some of the extracts were not effective. All the extracts reduced the size of the infected areas. CONCLUSION: The results propose myrtle extracts as a possible natural alternative to synthetic fungicides. © 2021 Society of Chemical Industry.


Assuntos
Citrus/microbiologia , Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Fungicidas Industriais/farmacologia , Myrtus/química , Penicillium/efeitos dos fármacos , Doenças das Plantas/prevenção & controle , Extratos Vegetais/farmacologia , Conservação de Alimentos/instrumentação , Conservantes de Alimentos/química , Armazenamento de Alimentos , Frutas/microbiologia , Penicillium/classificação , Penicillium/crescimento & desenvolvimento , Doenças das Plantas/microbiologia , Extratos Vegetais/química , Folhas de Planta/química
5.
Acta Sci Pol Technol Aliment ; 20(1): 25-35, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33449517

RESUMO

BACKGROUND: Sugar refineries cannot modernize the current processing technology and increase their capacity in proportion to the increasing harvest of raw beets. This entails an increase in the processing time. Sugar beets are not subject to long-term storage, and when they are stored in inappropriate conditions, root crops rot, resulting in sugar loss. The aim of this study is to increase the safety of beets during long-term storage before processing and to develop a device for its implementation which will lead to an improvement in the biological value of sugar beet root crops and an increase in the efficiency of technological processes. METHODS: The experiment used sugar beets from the Koksu sugar plant and was carried out by treating sugar beets with an ion-ozone mixture to increase their shelf life. The treatment was carried out in an ion-ozone installation. Physicochemical and microbiological analyses were carried out using several methods: chemical extraction, potentiometry and photocolorimetry. RESULTS: The results of the study showed that when sugar beets were treated with ozone at a concentration of 0.5 g/m3 and 2 g/m3, the acidity decreased to 0.6 degrees, and the sugar content increased by 2.3% and 3.3%, respectively. When sugar beets were processed with an ozone concentration of 2 g/m3 and a molecular ion concentration of 1,000,000 units/cm3, a decrease in moisture was observed to 69%, the acidity decreased 2 times and the sugar content increased by 3%. When the beets were processed with an ozone concentration of 2 g/m3 and a molecular ion concentration of 1,000,000 units/cm3, a decrease in acidity was observed to 0.65-0.67 degrees, and the sugar content increased by 2-2.5%. Also, in all the above optimal processing conditions, a decrease in yeast growth was observed. CONCLUSIONS: As a result of the study, the following three optimal conditions were established for the processing of sugar beet root crops before storage: an ozone concentration of 0.5 g/m3 and 2 g/m3; an ozone concentration of 5 mg/m3 and molecular ions of 500,000 units/cm3; an ozone concentration of 2 g/m3 and molecular ions of 1,000,000 units/cm3.


Assuntos
Beta vulgaris , Conservantes de Alimentos/farmacologia , Armazenamento de Alimentos , Ozônio/farmacologia , Análise de Alimentos , Conservação de Alimentos/instrumentação , Conservação de Alimentos/métodos , Conservantes de Alimentos/química , Ozônio/química
6.
J Sci Food Agric ; 101(11): 4473-4480, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33432579

RESUMO

BACKGROUND: The greatest hurdle to commercial marketing of fresh-cut fruits and vegetables is limited shelf life due to microbial hazards and quality deterioration. Atmospheric cold plasma (ACP) is an emerging non-thermal technology with significant potential to improve the safety and storability of fresh products. The objective of this study was to evaluate the effects of ACP, generated in sealed packaging, on the qualitative, metabolic and microbial stability of fresh-cut pears during simulated cold storage. RESULTS: ACP treatments were effective in inhibiting the growth of mesophilic aerobic bacteria, yeast and mold, particularly CP3 (65 kV, 1 min), which could prolong shelf life to the greatest extent. While decontamination was not always associated with an increase in plasma intensity. Moreover, at 65 kV for 1 min, ACP treatment had the potential to retard respiration, and maintain organoleptic properties and other quality attributes. Additionally, peroxidase and pectin methylesterase (PME) activities were reduced immediately after treatments. These effects were dependent on treatment voltage and time, while a subsequent recovery in activity was only observed for PME. CONCLUSION: The results obtained from this study will contribute to an understanding of the effects of in-package ACP treatments on the storability and microbial safety of fresh-cut pears. This knowledge could be beneficial in reducing quality losses for fresh-cut pears and the preservation of other products. © 2021 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Frutas/química , Gases em Plasma/farmacologia , Pyrus/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Hidrolases de Éster Carboxílico/metabolismo , Embalagem de Alimentos , Conservação de Alimentos/instrumentação , Frutas/metabolismo , Frutas/microbiologia , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Peroxidase/metabolismo , Proteínas de Plantas/metabolismo , Pyrus/química , Pyrus/microbiologia , Controle de Qualidade
7.
J Sci Food Agric ; 101(3): 853-862, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32726459

RESUMO

BACKGROUND: 'Patharnakh' pear, a dominant cultivar in the Punjab province of India, has a shorter storage life as it matures during the hot and humid weather. Studies have reported that postharvest chemical treatments have a major role in improving the storage life of the fruit. In this study, the efficacy of different concentrations (1, 2, and 3 mmol L-1 ) of salicylic acid (SA), a well-known signaling molecule, was explored to overcome browning and maintain the postharvest quality of the Patharnakh pear during cold storage. RESULTS: SA treated pears were better than the untreated fruits in all of the studied parameters. SA application alleviated the rate of weight loss and respiration, and lowered the decay percentage. The efficacy of SA in the reduction of polyphenol oxidase (PPO) activity, which correlates negatively with the total phenolic content, was quite noticeable. The SA treated fruits exhibited a slower oxidation of the total phenol content by inhibiting the action of PPO and retaining the total phenolic content, leading to lower incidence of browning. SA effectively maintained the ascorbic acid content and superoxide dismutase activity. Total soluble solids, titratable acidity, ascorbic acid, and pH of the pears were highest with the SA treatment. CONCLUSION: The 2 mmol L-1 SA treatment exhibited the best result of reducing fruit decay and tissue browning, and maintaining the postharvest quality parameters of pear up to 60 days of cold storage. © 2020 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Frutas/efeitos dos fármacos , Pyrus/química , Ácido Salicílico/farmacologia , Catecol Oxidase/metabolismo , Temperatura Baixa , Cor , Conservação de Alimentos/instrumentação , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Índia , Oxirredução , Fenóis/química , Fenóis/metabolismo , Proteínas de Plantas/metabolismo , Pyrus/efeitos dos fármacos , Pyrus/metabolismo
8.
J Sci Food Agric ; 101(9): 3778-3786, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33300626

RESUMO

BACKGROUND: This study was conducted in two parts to improve the antimicrobial activity and stability of Satureja hortensis essential oil (SEO) and its impacts on the quality of pomegranate arils. In the first part, SEO was encapsulated by an ionic gelation technique into 142.2-267.7 nm chitosan nanoparticles (CSNPs). In the second part of the experiment, the CSNPs and CSNPs-SEO were applied to improve storability of pomegranate arils. Arils were dipped in water (control), CSNPs and CSNPs-SEO for 5 min. After superficial water removal, arils were packed into polystyrene boxes and stored at 5 °C for 18 days. RESULTS: Based on spectrophotometry analysis, the encapsulation efficiency (EE) of SEO-loaded CSNPs (CSNPs-SEO) decreased from 26.57% to 7.41% and their loading capacity (LC) increased from 4.72% to 6.17%, respectively, upon increasing the initial SEO content from 0.125 to 0.5 g g-1 of chitosan. Phytochemicals and water content were maintained, and microbial counts were reduced in the coated arils during storage. Total phenol and antioxidant activity decreased during storage. At the end of storage, the highest total phenol content (2980.0 mg gallic acid equivalents L-1 ) was found in arils treated with CSNPs-SEO, whereas ascorbic acid content was maximal (6.32 mg L-1 ) in arils treated with CSNPs. The encapsulation of savory essential oil in chitosan nanoparticles did not have undesirable effects in pomegranate arils. CONCLUSION: Pre-storage treatment of pomegranate arils with CSNPs-SEO could be considered a beneficial treatment to better maintain the biochemical and sensorial quality during storage. © 2020 Society of Chemical Industry.


Assuntos
Quitosana/química , Conservação de Alimentos/métodos , Conservantes de Alimentos/química , Frutas/química , Óleos Voláteis/química , Óleos de Plantas/química , Punica granatum/química , Satureja/química , Antocianinas/análise , Antioxidantes/análise , Ácido Ascórbico/análise , Quitosana/farmacologia , Conservação de Alimentos/instrumentação , Conservantes de Alimentos/farmacologia , Armazenamento de Alimentos , Nanopartículas/química , Óleos Voláteis/farmacologia , Fenóis/análise , Óleos de Plantas/farmacologia
9.
J Sci Food Agric ; 101(9): 3564-3574, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33280107

RESUMO

BACKGROUND: Granny Smith (GS) apple has low protein content and poor antimicrobial properties; hence it has been blended with Aloe vera (AV; high ascorbic acid, antimicrobial and antioxidant properties) and soybean flour (SF; rich in phenols, flavonoids, ascorbic acid, total antioxidant and protein) in different proportions to obtain fortified GS, i.e. GSAVSF. Moreover, GS being a perishable fruit, its moisture content should be reduced to enhance shelf life. Accordingly, this GSAVSF was osmotically pre-dehydrated and finally dried through energy-efficient quartz-halogen radiation (QHR) assisted vacuum-drying (QHRVD) to produce dried GSAVSF i.e. (DGSAVSF) under optimized conditions. RESULTS: The optimally dehydrated DGSAVSF product resulted in minimum moisture (4.85% w/w) and maximum protein (6.24 g kg-1 ) content. The application of osmotic dehydration and QHRVD afforded acceptable colour of DGSAVSF compared to GSAVSF (ΔEI * = 10.07 ± 0.21). A parametric drying model was formulated that corroborated well with Fick's equation. QHRVD rendered high moisture diffusivity (1.49 × 10-8 m2 s-1 ) and low activation energy (27.64 kJ mol-1  K-1 ). Appreciable quality improvements with respect to fresh GS concerning ascorbic acid (176.05%), total phenolic (579.07%), total flavonoid (333.33%) contents and 2,2'-diphenyl-1-picrylhydrazyl radical scavenging activity (446.71%) could be achieved. The product demonstrated satisfactory shelf life (1 × 104 CFU g-1 : aerobic mesophilic; 1 × 104 CFU g-1 : mould and yeast) and high rehydration ratio (4.25 ± 0.1). CONCLUSION: The enrichment of GS with AV and SF along with optimal drying protocols could provide a quality fortified DGSAVSF through an energy-proficient sustainable process. The highly nutritious product with suitable colour, microbial stability and rehydration ratio also satisfied a 9-point hedonic scale, thus confirming consumer acceptability. © 2020 Society of Chemical Industry.


Assuntos
Aloe/química , Dessecação/métodos , Farinha/análise , Conservação de Alimentos/métodos , Glycine max/química , Halogênios/química , Malus/química , Quartzo/química , Dessecação/instrumentação , Conservação de Alimentos/instrumentação , Alimentos Fortificados/análise , Frutas/química , Cinética , Vácuo
10.
J Sci Food Agric ; 101(2): 703-717, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32710440

RESUMO

BACKGROUND: Ziziphus jujuba Miller cv. Dongzao is extremely susceptible to reddening, browning, nutritional loss, and perishability after harvest. In this study, we evaluated the mechanisms of calcium chloride and chitosan/nano-silica composite film treatments on the quality, especially in reddening, by physiological and metabolomic assays. RESULTS: The treatment delayed the decline of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and chalcone isomerase (CHI) activities. Meanwhile, the treated groups retarded the increases in anthocyanin and quercetin contents by inhibiting the gene expressions of flavonol synthase (ZjFLS), dihydroflavonol 4-reductase (ZjDFR), and anthocyanidin synthase (ZjANS), while promoting leucoanthocyanidin reductase (ZjLAR) expression, which leads to retardation of fruit reddening. Anthocyanins were found to be responsible for post-harvest winter jujube reddening through principal component analysis. Results from the technique for order preference by similarity to an ideal solution indicated that the treated group delayed the decline of the quality of 'Dongzao' and extended its shelf life. CONCLUSION: The treatment induced the heightening of flavonoids metabolism. They enhanced the nutritional value and the ability to resist stress by delaying the decline of PAL, CHS, and CHI activities. Meanwhile, the treated groups retarded the increase in anthocyanin and quercetin contents by inhibiting the gene expressions of ZjFLS, ZjDFR, and ZjANS and promoting ZjLAR expression, which leads to retardation of fruit reddening. Anthocyanins are responsible for post-harvest winter jujube reddening. Coating treatment effectively delayed the decline of winter jujube quality. © 2020 Society of Chemical Industry.


Assuntos
Cloreto de Cálcio/farmacologia , Conservação de Alimentos/métodos , Frutas/química , Ziziphus/efeitos dos fármacos , Antocianinas/análise , Antocianinas/metabolismo , Conservação de Alimentos/instrumentação , Frutas/efeitos dos fármacos , Frutas/enzimologia , Frutas/genética , Regulação da Expressão Gênica de Plantas , Oxirredutases/genética , Oxirredutases/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Fenilalanina Amônia-Liase/genética , Fenilalanina Amônia-Liase/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Quercetina/análise , Quercetina/metabolismo , Ziziphus/química , Ziziphus/enzimologia , Ziziphus/genética
11.
J Sci Food Agric ; 101(7): 2809-2817, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33140406

RESUMO

BACKGROUND: Having short drying time and attractive product quality are important in fruit and vegetable dehydration processing. In this work, tri-frequency (20, 40 and 60 kHz) ultrasound-ethanol pretreatment, ultrasound-water pretreatment and ethanol pretreatment were employed before infrared convection drying (ICD) of scallion stalks, which was aimed at improving the drying process and quality of the end products. The mass transfer, drying characteristics (moisture ratio and drying rate and quality properties of scallion (rehydration, color, flavor, optical microscope image, moisture distribution and microbiological quality) were analyzed. RESULTS: All pretreatments have decreased the drying time by 33.34-83.34% compared to the control, while ultrasound-ethanol pretreatment provided the highest time reduction (83.34%). The reason is that the volatility of ethanol have replaced air in the tissue, which produced a better osmotic dehydration effect and the cavitation effect of ultrasound changed the cell function of the material, so that the food tissue was rapidly compressed and expanded, resulting in damage to the cell structure. Ultrasonic-ethanol pretreatment has greatly reduced the water loss and dry matter of fresh scallion, improved the rehydration effect of dried scallion, better retained the color and flavor of scallion and effectively reduced the microbiological quality of the scallion. CONCLUSION: The tri-frequency ultrasound-ethanol pretreatment has effectively improved the drying process and quality characteristics of the dried scallion. Therefore, this research has a great contribution to the drying technology, as evident in the remarkable reduction in drying time and the improvement in the quality of the end product. © 2020 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Cebolas/química , Cebolas/efeitos da radiação , Dessecação/instrumentação , Etanol/química , Conservação de Alimentos/instrumentação , Caules de Planta/química , Caules de Planta/efeitos da radiação , Ondas Ultrassônicas
12.
Food Microbiol ; 92: 103590, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32950134

RESUMO

Peroxyacetic acid (PAA) is a commonly used antimicrobial in apple spray bar interventions during post-harvest packing. However, limited information is available about its efficacy against foodborne pathogens on fresh apples under commercial packing conditions. In this study, the practical efficacies of PAA against Listeria monocytogenes on fresh apples during spray bar operation at ambient and elevated temperature were validated in three commercial packing facilities using Enterococcus faecium NRRL B-2354 as a surrogate strain. Apples were inoculated with E. faecium at ~6.5 Log10 CFU/apple and subjected to PAA spray bar interventions per commercial packing line practice. At each temperature and contact time intervention combination, 20-24 inoculated apples were processed together with 72-80 non-inoculated apples. Applying 80 ppm PAA at ambient temperature (17-21 °C) achieved a similar log reduction (P > 0.05) of E. faecium on Granny Smith apples (GSA) in three apple packing facilities, which caused 1.12-1.23 and 1.18-1.32 Log10 CFU/apple reductions of E. faecium on GSA for 30-sec and 60-sec intervention, respectively. Increasing the temperature of the PAA solution to 43-45 °C enhanced its bactericidal effect against E. faecium, causing 1.45, 1.86 and 2.19 Log10 CFU/apple reductions in three packing facilities for a 30-sec contact, and 1.50, 2.24, and 2.29 Log10 CFU/apple reductions for a 60-sec contact, respectively. Similar efficacies (P > 0.05) of PAA at both ambient and elevated temperature were also observed on Fuji apples. Spraying PAA on apples at ambient or elevated temperature reduced the level of E. faecium cross-contamination from inoculated apples to non-inoculated apples but could not eliminate cross-contamination. Data from this study provides valuable technical information and a reference point for the apple industry in controlling L. monocytogenes and verifying the effectiveness of their practices.


Assuntos
Enterococcus faecium/efeitos dos fármacos , Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Listeria monocytogenes/efeitos dos fármacos , Ácido Peracético/farmacologia , Enterococcus faecium/crescimento & desenvolvimento , Microbiologia de Alimentos , Conservação de Alimentos/instrumentação , Frutas/microbiologia , Listeria monocytogenes/crescimento & desenvolvimento , Malus/microbiologia
13.
Food Microbiol ; 92: 103583, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32950167

RESUMO

Combined use of biocontrol agents and plant extracts can be considered a viable and promising strategy for protecting plant tissues with different synergistic mechanisms of action that improve the antimicrobial activity of the mixtures. Treatments of citrus fruits with Wickerhamomyces anomalus BS91 have been previously reported as effective measures to reduce the incidence of green mold caused by Penicillium digitatum. On the opposite, the knowledge of the antifungal activity of cultivated cardoon (Cynara cardunculus L. var. altilis DC.) leaf extract, vegetable widespread in some Mediterranean areas, is still very limited. In this study, experimental trials were conducted to evaluate the effectiveness in vitro of leaf aqueous, methanolic and ethanolic extracts of C. cardunculus against seven fungal pathogens responsible for considerable food losses in the postharvest stage. In addition, biocontrol yeast W. anomalus BS91 and the three C. cardunculus extracts were tested in vivo both as a single treatment and in mixture, against Penicillium digitatum on 'Tarocco' oranges and 'Femminello' lemons. The combination of W. anomalus BS91 and leaf ethanolic extract reduced with the highest efficacy the incidence and severity of green mold on orange and lemon fruits with respect to the control, and was more effective than treatment with antagonistic yeast or leaf extracts applied alone. Incidence and severity of citrus decay were more consistently reduced when mixtures were applied 24 h before the inoculation of the pathogen, thus suggesting the relevance of preventive treatments. The mixtures of antagonistic W. anomalus BS91 and ethanolic leaf extract were more effective in controlling green mold decay on oranges than on lemons. These results indicate that biocontrol agents and leaf extracts, used in appropriate combination, can provide a stronger protection than when used singularly. However, compatibility between microbial antagonist and antimicrobial extract should be preliminary verified.


Assuntos
Antibiose , Citrus/microbiologia , Conservação de Alimentos/métodos , Extratos Vegetais/farmacologia , Saccharomycetales/fisiologia , Agentes de Controle Biológico/farmacologia , Citrus/química , Cynara/crescimento & desenvolvimento , Cynara/fisiologia , Conservação de Alimentos/instrumentação , Frutas/microbiologia , Penicillium/efeitos dos fármacos , Penicillium/crescimento & desenvolvimento , Penicillium/fisiologia , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle
14.
J Sci Food Agric ; 100(15): 5586-5595, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32608515

RESUMO

BACKGROUND: Blueberry is universally acknowledged as a kind of berry rich in antioxidants. Cold plasma, an emerging non-thermal treatment technology, has been proved to be able to maintain or improve the antioxidant level while inactivating the microorganisms on the surface of fruits and vegetables. Postharvest blueberries were treated with atmospheric cold plasma (ACP; 12 kV, 5 kHz) for 0 s (Control), 30 s (ACP-30), 60 s (ACP-60), and 90 s (ACP-90) in this study, and the effects of ACP on the antimicrobial properties, antioxidant activities, and reactive oxygen species (ROS) production were investigated during storage at 4 ± 1 °C for 40 days. RESULTS: Total aerobic bacteria and mold populations on ACP-treated blueberries decreased significantly in a time-dependent manner (P < 0.05), and decreased by 0.34-1.24 and 0.57-0.87 log10 CFU g-1 respectively on ACP-60-treated blueberries during storage. The decay rate of blueberries was decreased by 5.8-11.7% and the decrease of blueberry firmness was slowed down by ACP-60. But the total phenol, anthocyanin, and ascorbic acid contents increased, and superoxide dismutase, catalase, and peroxidase activities were enhanced in ACP-treated blueberries. The free radical scavenging activity and total antioxidant capacity (T-AOC) were enhanced. Hydrogen peroxide (H2 O2 ) and superoxide anion (O2 - ) production rates declined by 27.3% and 41.3% at day 40 of storage, respectively. CONCLUSION: It is suggested that ACP may be a promising non-thermal treatment technology for postharvest sterilization and preservation of blueberry under suitable conditions. © 2020 Society of Chemical Industry.


Assuntos
Antioxidantes/análise , Mirtilos Azuis (Planta)/química , Conservação de Alimentos/métodos , Frutas/efeitos dos fármacos , Gases em Plasma/farmacologia , Antocianinas/análise , Antocianinas/metabolismo , Ácido Ascórbico/análise , Ácido Ascórbico/metabolismo , Bactérias/crescimento & desenvolvimento , Mirtilos Azuis (Planta)/efeitos dos fármacos , Mirtilos Azuis (Planta)/metabolismo , Mirtilos Azuis (Planta)/microbiologia , Catalase/metabolismo , Conservação de Alimentos/instrumentação , Armazenamento de Alimentos , Frutas/química , Frutas/metabolismo , Frutas/microbiologia , Fungos/crescimento & desenvolvimento , Peróxido de Hidrogênio/análise , Peróxido de Hidrogênio/metabolismo , Fenóis/análise , Fenóis/metabolismo , Proteínas de Plantas/metabolismo , Espécies Reativas de Oxigênio/análise , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
15.
Poult Sci ; 99(1): 536-545, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32416840

RESUMO

The objective of the present study was to assess the potential synergistic effect between supercritical carbon dioxide (SC-CO2) and fresh culinary herbs (Coriandrum sativum and Rosmarinus officinalis) on the microbial inactivation of raw chicken meat. The microbiological inactivation was performed on Escherichia coli and natural flora (total mesophilic bacteria, yeasts, and molds). High pressure treatments were carried out at 40°C, 80 or 140 bar from 15 to 45 min. Microbial inactivation had a strong dependence on treatment time, achieving 1.4 log CFU/g reduction of E. coli after 15 min, and up to 5 log after 45 min, while a pressure increase from 80 up to 140 bar was not significant on the microbial inactivation. Mesophilic microorganisms were strongly reduced (>2.6 log CFU/g) after 45 min, and yeasts and molds were below the detection limits of the technique (<100 CFU/g) in most cases. The combination of fresh herbs together with SC-CO2 treatment did not significantly increase the inactivation of either E. coli or natural flora, which was similar to the SC-CO2 alone. The synergistic effect was obtained on the inactivation of E. coli using a proper concentration of coriander essential oil (EO) (0.5% v/w), while rosemary EO did not show a significant effect. Color analysis after the treatment showed an increment of lightness (L*), and a decrease of redness (a*) on the surface of the sample, making the product visually similar to cooked meat. Texture analysis demonstrated the modification of the texture parameters as a function of the process pressure making the meat more similar to the cooked one.


Assuntos
Dióxido de Carbono/análise , Microbiologia de Alimentos/métodos , Conservação de Alimentos/métodos , Carne/microbiologia , Viabilidade Microbiana , Óleos Voláteis/administração & dosagem , Animais , Galinhas , Escherichia coli/fisiologia , Microbiologia de Alimentos/instrumentação , Conservação de Alimentos/instrumentação , Fungos/fisiologia , Óleos de Plantas/administração & dosagem , Pressão , Leveduras/fisiologia
16.
J Food Sci ; 85(2): 432-441, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31968399

RESUMO

Infrared radiation (IR)-assisted peeling is one of the effulent free, environtment friendly emerging technique for tender fruit and vegetables. In this study standerdization and optimization of the infrared assisted dry-peeling method was carried out for ginger rhizome. During the investigation, the effects of selected independent parameters like infrared temperature (300-400 °C), heater to product surface spacing (10-30 mm), and treatment time (120-300 s) were studied on the peeling feasibility and quality as dependent variables. The experimental conditions were designed through CCRD statistical method. Multiple response optimization was done through RSM. The optimum conditions of selected independent variables were 300 °C IR temperature, 21 mm heater to product spacing, and 120 seconds treatment time resulted in the 90.40% of peeling efficiency, 35 °C of rhizome surface temperature, 8.67% of color change, 0.56% volatile oil loss, and 11.53 kg of firmness. The comparison of optimized infrared assisted peeling was carried out with conventional abrasion and lye peeling methods based on their quality attributes. IR assisted dry-peeling results in minimum quality losses, higher peeling efficiency and feasible over conventional peeling methods of ginger rhizome. There was zero effluent generated during infrared assisted peeling of ginger rhizome. PRACTICAL APPLICATION: Peeling is one of the foremost mandatory unit operation for processing of all fruits, vegetables, and rhizomes like ginger. Presently, lye peeling is most widely used peeling method in industries followed by mechanical peeling. Both of these methods have serious issues like huge effluent generation (BOD) and water consumption which leads to the concern about environmental issues. Infrared dry peeling is the most prominent alternative for industries having the potential to deals with environmental issues. IR peeling method can be easily designed and scaled-up as per the specific requirment of industries. Therefore, understanding its feasibility for peeling of a most difficult commodity like ginger and understanding its insight into the quality of peeled product is need of the hour.


Assuntos
Conservação de Alimentos/métodos , Rizoma/efeitos da radiação , Zingiber officinale/química , Estudos de Viabilidade , Conservação de Alimentos/instrumentação , Zingiber officinale/efeitos da radiação , Raios Infravermelhos , Óleos Voláteis/química , Extratos Vegetais/química , Rizoma/química
17.
J Sci Food Agric ; 100(5): 1897-1903, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31825530

RESUMO

BACKGROUND: Sapota is a popular tropical fruit characterized by a very short postharvest life. Low-temperature storage prolongs postharvest life of sapota fruit, but chilling injury symptoms can develop if the storage temperature is less than 14 °C. There have been no reports on the effects of postharvest melatonin application on the development of chilling injury in sapota fruit during cold storage. The objective of this study was to investigate the effects of different concentrations of postharvest melatonin application (0, 30, 60 and 90 µmol L-1 ) during cold storage (8 °C) for up to 30 days with an additional 1-day shelf life at ambient temperature. RESULTS: All melatonin treatments reduced chilling injury symptoms, reduced electrolyte leakage, malondialdehyde (MDA) content, H2 O2 and superoxide anion (O2 - ), and increased proline content and the activity of superoxide dismutase (SOD), catalase (CAT) and γ-aminobutyric acid (GABA), and reduced the activities of phospholipase D (PLD) and lipoxygenase (LOX) compared to the control. CONCLUSION: Postharvest melatonin treatment could be a useful strategy for reducing chilling injury during cold storage and transport of sapota fruit. The results indicate that melatonin reduces chilling injury of sapota fruit through maintaining membrane integrity, SOD and CAT activities, and reducing PLD and LOX activities. © 2019 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Melatonina/farmacologia , Catalase/metabolismo , Temperatura Baixa , Conservação de Alimentos/instrumentação , Armazenamento de Alimentos , Frutas/química , Frutas/efeitos dos fármacos , Frutas/metabolismo , Malondialdeído/metabolismo , Manilkara/química , Manilkara/efeitos dos fármacos , Manilkara/metabolismo , Ácido gama-Aminobutírico/metabolismo
18.
J Sci Food Agric ; 100(3): 961-968, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31591725

RESUMO

BACKGROUND: Among the challenges for postharvest researchers is that of understanding the physiological and biochemical pathways associated with postharvest fruit decay. Fruit senescence directly affects sensorial and nutritional quality during postharvest life. It has been clarified that reactive oxygen species and oxidative damage are responsible for fruit senescence. Some cultivars of yellow-fleshed kiwifruit can be stored for a short period compared with green-fleshed kiwifruit. Postharvest performance is affected by the physiological state of the fruit at harvest, associated with its postharvest management. Among several postharvest applications, ozone treatment is considered as a cost-effective and eco-friendly food-processing technology to preserve the fruits' quality during cold storage. In this study, we investigated the influence of ozone, after gradual cooling treatment, on the antioxidant defense system in Actinidia chinensis, 'Soreli'. RESULTS: Bioactive compound content decreased during cold storage, and ozone treatment enhanced the activities of superoxide dismutase and catalase during cold storage. This treatment preserved membrane integrity by inhibiting lipoxygenase activity and malondialdehyde accumulation. A multivariate statistical approach, using principal component analysis, provided the global response to the effect of ozone postharvest treatment during cold storage in kiwifruit 'Soreli'. CONCLUSION: Ozone treatment improves the efficiency of antioxidative system and storability of 'Soreli' kiwifruits. © 2019 Society of Chemical Industry.


Assuntos
Actinidia/química , Antioxidantes/análise , Conservação de Alimentos/métodos , Frutas/efeitos dos fármacos , Ozônio/farmacologia , Actinidia/efeitos dos fármacos , Actinidia/crescimento & desenvolvimento , Actinidia/metabolismo , Antioxidantes/metabolismo , Cor , Conservação de Alimentos/instrumentação , Frutas/química , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Malondialdeído/metabolismo , Proteínas de Plantas/metabolismo , Superóxido Dismutase/metabolismo
19.
J Food Sci ; 84(12): 3700-3706, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31721204

RESUMO

The antibacterial effects of ultrasound (US) and cinnamon essential oil (CEO), individually and combined, were investigated against Listeria monocytogenes and Salmonella Typhimurium in low- and high-fat milk during 6-day storage. At the end of storage, CEO alone decreased 2 and 2.2 log cycles of Salmonella Typhimurium and 2.5 and 3 log cycles of L. monocytogenes populations in low- and high-fat milk, respectively. US alone reduced 1.6 log cycle of Salmonella Typhimurium and 0.7 log cycle of L. monocytogenes in both milk type. The combined treatment could reduce 2.7 log cycle of Salmonella Typhimurium in low-fat milk and 3.8 log cycle in high-fat milk. The combined treatment also achieved 4.3 and 4.5 log cycle reductions of L. monocytogenes in low- and high-fat milk, respectively. The results of this study showed that the combination of CEO and US could be used as an effective antibacterial treatment in milk. PRACTICAL APPLICATIONS: Due to adverse effects of thermal processing on the sensory and nutritional properties of food and the potentially harmful effects of chemical preservatives, nonthermal preservation methods and natural antimicrobials have been gained much attention. In this study, the antibacterial effects of ultrasound (US) and cinnamon essential oil (CEO), individually and combined, were investigated against Listeria monocytogenes and Salmonella Typhimurium in low- and high-fat milk. The results indicated that combination of US and CEO could significantly decrease L. monocytogenes and Salmonella Typhimurium populations in milk. Then, this combined treatment may be used as an effective alternative method to microbial inactivation in milk.


Assuntos
Antibacterianos/farmacologia , Cinnamomum zeylanicum/química , Conservação de Alimentos/métodos , Leite/microbiologia , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Ultrassom/métodos , Animais , Antibacterianos/isolamento & purificação , Bovinos , Contagem de Colônia Microbiana , Conservação de Alimentos/instrumentação , Listeria monocytogenes/efeitos dos fármacos , Listeria monocytogenes/crescimento & desenvolvimento , Viabilidade Microbiana/efeitos dos fármacos , Leite/química , Óleos Voláteis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/crescimento & desenvolvimento
20.
J Food Sci ; 84(11): 3194-3203, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31589346

RESUMO

A multilayer film containing star anise essential oil and thymol coating layers (SAEO and TH, respectively), with insect repellent and antimicrobial properties, has been developed using bar coating and adhesive lamination processes. Our previous study reported the in vitro activities of this polypropylene film (PP)/SAEO/polyethylene terephthalate film (PET)/TH/low-density polyethylene film (LDPE) multilayer film. The current study focused on demonstrating the morphological, optical, and mechanical properties of the film, and evaluating its in vivo activities when used as a bread packaging material. The developed film was 15.03% thicker and 1.86% less transparent than the control film (without active agent coating layers: PP/PET/LDPE). While the color values of the developed film were slightly different from the control film, both films appeared similar to the naked eye. The tensile strength in the developed film was somewhat lower than that of the control film, while both films had statistically comparable values for elongation at break. During storage of sliced bread packaged in the developed film, the film both deterred insects from approaching toward and impeded the growth of microorganisms in the bread. These results suggest the potential applicability of the developed film as an active food packaging material with insect repellent and antimicrobial activities. PRACTICAL APPLICATION: A multilayer film incorporated with insect repellent and antimicrobial coating layers was applied in sliced wheat bread packaging. The developed film effectively inhibited approaches of stored-product insects to packaged bread and growth of microorganisms on the bread surface. It can be used as an active food packaging material that improves the safety and shelf-life of foods.


Assuntos
Antibacterianos/química , Pão/análise , Conservação de Alimentos/métodos , Illicium/química , Repelentes de Insetos/química , Óleos Voláteis/química , Timol/química , Animais , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Pão/microbiologia , Embalagem de Alimentos/instrumentação , Conservação de Alimentos/instrumentação , Repelentes de Insetos/farmacologia , Insetos/efeitos dos fármacos , Insetos/crescimento & desenvolvimento , Óleos Voláteis/farmacologia , Óleos de Plantas/química , Óleos de Plantas/farmacologia , Polietileno/química , Polipropilenos/química , Timol/farmacologia , Triticum/química , Triticum/microbiologia
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