RESUMO
Polycyclic aromatic hydrocarbons (PAHs) are food-processing contaminants considered to be carcinogenic and genotoxic. Due to its drying process stage, teas may be contaminated with PAHs. The aim of the study was to validate an analytical method involving QuEChERS and HPLC-FLD for the determination of PAH4 in teas and evaluate the contamination levels in 10 different types of teas from Brazil. Recoveries varied from 54% to 99% and relative standard deviations from 1% to 21%. Limits of detection and quantification were from 0.03 to 0.3 µg/kg and 0.1 to 0.5 µg/kg, respectively. Mate tea presented the highest PAH levels, with PAH4 varying from 194 to 1795 µg/kg; followed by black (1.8-186 µg/kg), white (24-119 µg/kg), and green teas (3.1-92 µg/kg). Teas with lowest PAH4 were strawberry, lemongrass, peppermint, and boldo. Only trace levels of PAHs were detected in tea infusions, so apparently it would not affect PAH intake by Brazilian population.
Assuntos
Carcinógenos Ambientais/análise , Contaminação de Alimentos , Hidrocarbonetos Policíclicos Aromáticos/análise , Chá/química , Chás de Ervas/análise , Métodos Analíticos de Preparação de Amostras , Benzo(a)Antracenos/análise , Benzo(a)Antracenos/isolamento & purificação , Benzo(a)pireno/análise , Benzo(a)pireno/isolamento & purificação , Brasil , Carcinógenos Ambientais/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Crisenos/análise , Crisenos/isolamento & purificação , Fluorenos/análise , Fluorenos/isolamento & purificação , Manipulação de Alimentos , Inspeção de Alimentos/métodos , Ilex paraguariensis/química , Limite de Detecção , Oxirredução , Folhas de Planta/química , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Reprodutibilidade dos Testes , Espectrometria de Fluorescência , Chá/economia , Chás de Ervas/economiaRESUMO
Bio-guided fractionation of Aspergillus terreus extract leads to isolation of a novel terpenoidal secondary metabolite. The isolated compound and the total alcoholic extract of Aspergillus terreus showed a remarkable activity against microbial mouth infections; namely, Candida albicans, Lactobacillus acidophilus, Streptococcus gordonii, and S. mutan. Moreover, the Minimum Inhibitory Concentration of the isolated compound was determined and showed low values. The combination of each of the alcoholic extract of A. terreus and the isolated compound Coe-Comfort tissue conditioner inhibited the growth of Candida albicans at concentrations of 500 and 7.81 µg/mL, respectively, Lactobacillus acidophilus at concentrations of 250 and 7.81 µg/mL, respectively, Streptococcus gordonii at concentrations of 1000 and 62.50 µg/mL, respectively, and S. mutans at concentrations of 1000 and 125 µg/mL, respectively. The oral dosing of the extract and the isolated compound did not show any significant effect on the activity of alanine aminotransferase, aspirate aminotransferase, and the levels of blood urea and serum creatinine. Copyright © 2017 John Wiley & Sons, Ltd.