RESUMO
The bioactive chemicals in L. cuneata were investigated by repeated column chromatography and their effect on aldose reductase (AR), obtained from rat lenses, was examined. Results showed that the ethyl acetate and n-butanol fractions of L. cuneata exhibited potential inhibitory effect against AR with IC50 values of 0.57 and 0.49 µg/mL, respectively. Phytochemical analysis of these two fractions resulted in the isolation of five flavonoids namely, acacetin (1), afzelin (2), astragalin (3), kaempferol (4) and scutellarein 7-O-glucoside (5). The AR inhibitory effect of compounds 1-5 was explored; compounds 2, 3 and 5 showed potential AR-inhibitory effects with IC50 values of 2.20, 1.91 and 12.87 µM, respectively. Quantitative analysis of afzelin (2) and astragalin (3) in L. cuneata by high performance liquid chromatography with ultraviolet detection revealed its content to be 0.722-11.828 and 2.054-7.006 mg/g, respectively. Overall, this study showed that L. cuneata is rich in flavonoids with promising AR-inhibitory activities, which can be utilized for the development of natural therapies for treating and managing diabetic complications.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Flavonoides , Quempferóis , Lespedeza/química , Manosídeos , Proantocianidinas , Aldeído Redutase/metabolismo , Animais , Flavonoides/análise , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Quempferóis/análise , Quempferóis/isolamento & purificação , Quempferóis/farmacologia , Cristalino/enzimologia , Manosídeos/análise , Manosídeos/isolamento & purificação , Manosídeos/farmacologia , Extratos Vegetais/química , Proantocianidinas/análise , Proantocianidinas/isolamento & purificação , Proantocianidinas/farmacologia , Ratos , Ratos Sprague-DawleyRESUMO
BACKGROUND: Increased activity of aldose reductase (AR) is one of the mechanisms involved in the development of diabetic complications. Inhibiting AR can be a target to prevent diabetes complications. This study is aimed at evaluating the effect of cyclohexane (CH) and ethanol extracts (ET) of walnut leaves on AR activity in the lens and testis of diabetic rats. METHODS: Fifty-six male rats classified into seven groups as control and treatment groups and treated for 30 days. The treatment groups were treated with different concentrations of ET and CH. The diabetic control (DC) group was exposed to streptozotocin. AR activity was measured in the lens and testis. The expression of AR in the testis was evaluated by the immunohistochemistry method. RESULTS: Both extracts significantly reduced the AR activity (ng/mg of tissue protein) in the testis (0.034 ± 0.004, 0.038 ± 0.010, and 0.040 ± 0.007 in the treatment groups vs. 0.075 ± 0.007 in the DC group) and lens (1.66 ± 0.09, 2.70 ± 0.47, and 1.77 ± 0.20 in the treatment groups vs. 6.29 ± 0.48 in the DC group) of the treatment group compared to those of the DC group (P < 0.05). AR expression in the testes of the treatment groups was decreased compared with that of the DC group (P < 0.0001). CONCLUSION: Walnut leaf extracts can reduce the activity and localization of AR in the testes and its activity in the lens of diabetic rats.
Assuntos
Aldeído Redutase/metabolismo , Complicações do Diabetes/prevenção & controle , Diabetes Mellitus Experimental/enzimologia , Hipoglicemiantes/farmacologia , Cristalino/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Complicações do Diabetes/enzimologia , Hipoglicemiantes/uso terapêutico , Juglans , Cristalino/enzimologia , Masculino , Extratos Vegetais/uso terapêutico , Folhas de Planta , Ratos , Ratos Sprague-Dawley , Testículo/efeitos dos fármacos , Testículo/enzimologiaRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Lycium barbarum polysaccharide (LBP) extracted from the Lycium barbarum L. has been widely used to improve diabetes and its relative complications. However, the mechanisms have not fully understood. A recent study has demonstrated that LBP upregulates suituin 1 (SIRT1). OBJECTIVE: This study was to define the role of Sirt1 and its downstream signaling pathways in diabetic cataract using in vitro and in vivo models. MATERIALS AND METHODS: Human lens epithelial cell line SRA01/04 cells were cultured under high glucose (HG) medium with treatment of LBP or vehicle. Cell viability, apoptosis, protein and/or mRNA levels of Sirt1, BAX, Bcl-2, active-caspase-3, FOXO1, p27 and acetylated p53 were measured. SIRT1 upregulated- and knocked-down cells were generated and tested in high glucose culture. Diabetes mellitus was induced in rats by streptozotocin injection. Body weight, blood glucose levels, lens transparency and retinal function were assessed and SIRT1, as well as the aforementioned biomarkers were measured using Western blotting and qPCR in the animal lens samples. RESULTS: The results showed that HG decreased cell viability and LBP prevented the decrease. The reduced viability in HG cultured SRA01/04 cells was associated with increased levels of BAX, active caspase 3, FOXO1, p27, and p53 and decreased levels of SIRT1 and Bcl-2. Further experiments using sirt1 gene modulated cells showed that upregulation of Sirt1 improved viability, increase cell division as reflected by an increased proportion of S phase in the cell cycle, reduced the number of apoptotic cell death and suppressed p53 acetylation and caspase 3 activation. Opposite results were observed in SIRT1 knock-down cells. Treating diabetic animals with LBP reduced body weight loss and blood glucose content in diabetic animals. Similarly, LBP hindered the development of cataract in lenses and improved retinal function. The beneficial effect of LBP on diabetic cataract was associated with the supression of p53, caspase 3, FOXO1, BAX, p27 and elevation of SIRT1 and Bcl-2, which were consistent with the in vitro findings. CONCLUSION: Our findings showed that diabetes caused cataract is associated with suppression of SIRT1 and Bcl-2 and activation of other cell death related genes. LBP prevented diabetic cataract in animals by upregulating Sirt1 and Bcl-2 and suppressing cell death related genes.
Assuntos
Catarata/prevenção & controle , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Tipo 1/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Células Epiteliais/efeitos dos fármacos , Cristalino/efeitos dos fármacos , Lycium , Sirtuína 1/metabolismo , Animais , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/genética , Proteínas Reguladoras de Apoptose/metabolismo , Catarata/enzimologia , Catarata/etiologia , Catarata/patologia , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Tipo 1/complicações , Medicamentos de Ervas Chinesas/isolamento & purificação , Células Epiteliais/enzimologia , Células Epiteliais/patologia , Humanos , Cristalino/enzimologia , Cristalino/patologia , Lycium/química , Masculino , Ratos Sprague-Dawley , Transdução de Sinais , Sirtuína 1/genéticaRESUMO
Skin lesions, cataracts, and congenital anomalies have been frequently associated with inherited deficiencies in enzymes that synthesize cholesterol. Lanosterol synthase (LSS) converts (S)-2,3-epoxysqualene to lanosterol in the cholesterol biosynthesis pathway. Biallelic mutations in LSS have been reported in families with congenital cataracts and, very recently, have been reported in cases of hypotrichosis. However, it remains to be clarified whether these phenotypes are caused by LSS enzymatic deficiencies in each tissue, and disruption of LSS enzymatic activity in vivo has not yet been validated. We identified two patients with novel biallelic LSS mutations who exhibited congenital hypotrichosis and midline anomalies but did not have cataracts. We showed that the blockade of the LSS enzyme reaction occurred in the patients by measuring the (S)-2,3-epoxysqualene/lanosterol ratio in the forehead sebum, which would be a good biomarker for the diagnosis of LSS deficiency. Epidermis-specific Lss knockout mice showed neonatal lethality due to dehydration, indicating that LSS could be involved in skin barrier integrity. Tamoxifen-induced knockout of Lss in the epidermis caused hypotrichosis in adult mice. Lens-specific Lss knockout mice had cataracts. These results confirmed that LSS deficiency causes hypotrichosis and cataracts due to loss-of-function mutations in LSS in each tissue. These mouse models will lead to the elucidation of the pathophysiological mechanisms associated with disrupted LSS and to the development of therapeutic treatments for LSS deficiency.
Assuntos
Catarata/genética , Epiderme/patologia , Hipotricose/genética , Transferases Intramoleculares/genética , Cristalino/patologia , Adolescente , Animais , Catarata/congênito , Catarata/patologia , Colesterol/metabolismo , Análise Mutacional de DNA , Modelos Animais de Doenças , Epiderme/enzimologia , Saúde Holística , Humanos , Hipotricose/congênito , Hipotricose/patologia , Transferases Intramoleculares/metabolismo , Lanosterol/análise , Lanosterol/metabolismo , Cristalino/enzimologia , Masculino , Camundongos , Camundongos Knockout , Mutação , Linhagem , Sebo/química , Sequenciamento do ExomaRESUMO
Diabetes mellitus is a multisystemic metabolic disorder that may affect the eyes, kidneys, vessels, and heart. Chronic hyperglycemia causes non-enzymatic glycation of proteins and elevation of the polyol pathway resulting in oxidative stress that damages organs. The current study aimed to investigate the dose-dependent effects of orally consumed Rosa damascena Mill. hydrosol on hematology, clinical biochemistry, lens enzymatic activity, and lens pathology in streptozotocin (STZ)-induced diabetic rats. Diabetes was induced into male Sprague-Dawley rats by intraperitoneal administration of STZ (40 mg/kg body weight). Rose hydrosols containing 1515 mg/L and 500 mg/L total volatiles (expressed as citronellol) were introduced to rats orally for 45 days. Consumption of 1515 mg/L volatile containing rose hydrosol successfully ameliorated hematologic, hepatic, and renal functions. Hydrosols also attenuated hyperglycemia and decreased the advanced glycation end-product formation in a dose-dependent manner. Rose hydrosol components significantly increased the lens enzymatic activities of glutathione peroxidase and decreased the activity of aldose reductase to prevent cataractogenesis. Histopathological examinations of rat lenses also indicated that increasing the dose of rose hydrosol had a protective effect on lenses in diabetic conditions. Additionally, in silico modeling of aldose reductase inhibition with rose hydrosol volatiles was carried out for extrapolating the current study to humans. The present results suggest that rose hydrosol exerts significant protective properties in diabetes mellitus and has no toxic effect on all studied systems in healthy test groups.
Assuntos
Hematopoese/efeitos dos fármacos , Doenças do Cristalino/etiologia , Doenças do Cristalino/metabolismo , Cristalino/efeitos dos fármacos , Cristalino/enzimologia , Extratos Vegetais/farmacologia , Rosa/química , Animais , Sítios de Ligação , Biomarcadores , Testes de Química Clínica , Diabetes Mellitus Experimental , Modelos Animais de Doenças , Ativação Enzimática , Doenças do Cristalino/tratamento farmacológico , Cristalino/química , Masculino , Modelos Moleculares , Extratos Vegetais/química , Ligação Proteica , Conformação Proteica , RatosRESUMO
Aldose reductase (AR) is a drug target for therapies to treat complications caused by diabetes mellitus, and the development of effective AR inhibitors (ARIs) of natural origin is considered to be an attractive option for reducing these complications. In this research, the rat lens AR (RLAR) inhibitory activity of evening primrose (Oenothera biennis) seeds was investigated for the first time. In our results, the 50% (v/v) methanol extract of evening primrose seeds exhibits excellent RLAR inhibitory activity (IC50 value of 7.53 µg/mL). Moreover, after enrichment of its bioactive components, the ARIs are more likely to be present in the ethyl acetate fraction of 50% (v/v) methanol extract (EME) of evening primrose seeds, which exhibits superior RLAR inhibitory activity (IC50 value of 3.08 µg/mL). Finally, gallic acid (1), procyanidin B3 (2), catechin (3), and methyl gallate (4) were identified as the major ARIs from the EME by affinity-based ultrafiltration-high-performance liquid chromatography and were isolated by high speed countercurrent chromatography, with gallic acid (11.46 µmol/L) and catechin (14.78 µmol/L) being the more potent inhibitors of the four ARIs identified. The results demonstrated that evening primrose seeds may be a potent ingredient of ARIs.
Assuntos
Aldeído Redutase/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Oenothera biennis/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Sementes/química , Animais , Fracionamento Químico , Cromatografia Líquida de Alta Pressão , Avaliação Pré-Clínica de Medicamentos , Ativação Enzimática , Inibidores Enzimáticos/isolamento & purificação , Cristalino/enzimologia , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/isolamento & purificação , RatosRESUMO
The search for natural inhibitors with anti-diabetes properties has gained increasing attention. Among four selected Smilacaceae family plants, Smilax china L. stems (SCS) showed significant in vitro anti-glycation and rat lens aldose reductase inhibitory activities. Bioactivity-guided isolation was performed with SCS and four solvent fractions were obtained, which in turn yielded 10 compounds, including one phenolic acid, three chlorogenic acids, four flavonoids, one stilbene, and one phenylpropanoid glycoside; their structures were elucidated using nuclear magnetic resonance and mass spectrometry. All solvent fractions, isolated compounds, and stem extracts from plants sourced from six different provinces of South Korea were next tested for their inhibitory effects against advanced glycation end products, as well as aldose reductase. α-Glucosidase, and lipase assays were also performed on the fractions and compounds. Since compounds 3, 4, 6, and 8 appeared to be the superior inhibitors among the tested compounds, a comparative study was performed via high-performance liquid chromatography with photodiode array detection using a self-developed analysis method to confirm the relationship between the quantity and bioactivity of the compounds in each extract. The findings of this study demonstrate the potent therapeutic efficacy of SCS and its potential use as a cost-effective natural alternative medicine against type 2 diabetes and its complications.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Ácido Clorogênico/química , Produtos Finais de Glicação Avançada/antagonistas & inibidores , Glicosídeos/química , Hidroxibenzoatos/química , Smilax/química , Estilbenos/química , Aldeído Redutase/metabolismo , Animais , Ácido Clorogênico/isolamento & purificação , Ácido Clorogênico/farmacologia , Etanol/química , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Produtos Finais de Glicação Avançada/metabolismo , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Glicosilação/efeitos dos fármacos , Hidroxibenzoatos/isolamento & purificação , Hidroxibenzoatos/farmacologia , Cristalino/química , Cristalino/enzimologia , Lipase/antagonistas & inibidores , Lipase/metabolismo , Metanol/química , Estrutura Molecular , Extratos Vegetais/química , Caules de Planta/química , Ratos , Ratos Sprague-Dawley , Solventes/química , Estilbenos/isolamento & purificação , Estilbenos/farmacologia , alfa-Glucosidases/metabolismoRESUMO
Stauntonia hexaphylla (Thunb.) Decne. (Lardizabalaceae) leaves (SHL) have been used traditionally as analgesics, sedatives, diuretics, and so on, in China. To date, no data have been reported on the inhibitory effect of SHL and its constituents on rat lens aldose reductase (RLAR) and advanced glycation end products (AGEs). Therefore, the inhibitory effect of compounds isolated from SHL extract on RLAR and AGEs was investigated to evaluate potential treatments of diabetic complications. The ethyl acetate (EtOAC) fraction of SHL extract showed strong inhibitory activity on RLAR and AGEs; therefore, EtOAc fraction (3.0 g) was subjected to Sephadex LH-20 column chromatography, for further fractionation, with 100% MeOH solvent system to investigate its effect on RLAR and AGEs. Phytochemical investigation of SHL led to the isolation of seven compounds. Among the isolated compounds, chlorogenic acid, calceolarioside B, luteolin-3'-O-ß-D-glucopyranoside, quercetin-3-O-ß-D-glucopyranoside, and luteolin-7-O-ß-D-glucopyranoside exhibited significant inhibitory activity against RLAR with IC50 in the range of 7.34-23.99 µM. In addition, 3-(3,4-dihydroxyphenyl) propionic acid, neochlorogenic acid, and luteolin-3'-O-ß-D-glucopyranoside exhibited the most potent inhibitory activity against formation of AGEs, with an IC50 value of 115.07-184.06 µM, compared to the positive control aminoguanidine (820.44 µM). Based on these findings, SHL dietary supplements could be considered for the prevention and/or treatment of diabetes complication.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Antioxidantes/administração & dosagem , Extratos Vegetais/administração & dosagem , Traqueófitas/química , Aldeído Redutase/metabolismo , Animais , Antioxidantes/química , China , Produtos Finais de Glicação Avançada/metabolismo , Humanos , Cristalino/efeitos dos fármacos , Cristalino/enzimologia , Extratos Vegetais/química , Folhas de Planta/química , RatosRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as "Shihu" or "Huangcao". D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties. STUDY AIM: To characterize the interaction between gigantol extracted from D. chrysotoxum Lindl and the AR gene, and determine gigantol's efficacy against cataractogenesis. MATERIALS AND METHODS: Human lens epithelial cells (HLECs) were induced by glucose as the model group. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess AR gene expression. Then, the mode of interaction of gigantol with the AR gene was evaluated by UV-visible spectroscopy, atomic force microscope (AFM) and surface-enhanced Raman spectroscopy (SERS). The binding constant was determined by UV-visible. RESULTS: Gigantol depressed AR gene expression in HLECs. UV-visible spectra preliminarily indicated that interaction between the AR gene and gigantol may follow the groove mode, with a binding constant of 1.85×103L/mol. Atomic force microscope (AFM) data indicated that gigantol possibly bound to insert AR gene base pairs of the double helix. Surface-enhanced Raman spectroscopy (SERS) studies further supported these observations. CONCLUSION: Gigantol extracted from D. chrysotoxum Lindl not only has inhibitory effects on aldose reductase, but also inhibits AR gene expression. These findings provide a more comprehensive theoretical basis for the use of Dendrobium for the treatment of diabetic cataract.
Assuntos
Aldeído Redutase/genética , Bibenzilas/farmacologia , Catarata/prevenção & controle , Dendrobium/química , Guaiacol/análogos & derivados , Bibenzilas/isolamento & purificação , Catarata/etiologia , Células Cultivadas , Complicações do Diabetes/prevenção & controle , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/enzimologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glucose/metabolismo , Guaiacol/isolamento & purificação , Guaiacol/farmacologia , Humanos , Cristalino/citologia , Cristalino/efeitos dos fármacos , Cristalino/enzimologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise Espectral RamanRESUMO
In our ongoing efforts to identify effective naturally sourced agents for the treating of diabetic complications, two new (1 and 2) and 11 known phenolic compounds (3-13) were isolated from an 80â% ethanol extract of Litsea japonica leaves. The structures of the new compounds were established by spectroscopic and chemical studies. These isolates (1-13) were subjected to an in vitro bioassay evaluating their inhibitory activity on advanced glycation end products formation and rat lens aldose reductase activity. Of the compounds evaluated, the flavonoids (3, 4, 6-8, 11, and 12) markedly inhibited advanced glycation end products formation, with IC50 values of 7.4-72.0 µM, compared with the positive control, aminoguanidine (IC50 = 975.9 µM). In the rat lens aldose reductase assay, consistent with the inhibition of advanced glycation end products formation, the flavonoids (3, 4, 6-8, 11, and 12) exhibited considerable inhibition of rat lens aldose reductase activity, with IC50 values of 1.1-12.5 µM. In addition, the effects of kaempferol (4) and tiliroside (7) on the dilation of hyaloid-retinal vessels induced by high glucose in larval zebrafish were investigated. Only kaempferol significantly reduced the diameters of high glucose-induced hyaloid-retinal vessels, by 52.2â% at 10 µM, compared with those in the high glucose-treated control group.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Flavonoides/farmacologia , Produtos Finais de Glicação Avançada/antagonistas & inibidores , Cristalino/enzimologia , Litsea/química , Aldeído Redutase/metabolismo , Animais , Angiopatias Diabéticas/induzido quimicamente , Modelos Animais de Doenças , Flavonoides/química , Flavonoides/isolamento & purificação , Produtos Finais de Glicação Avançada/metabolismo , Guanidinas/farmacologia , Técnicas In Vitro , Concentração Inibidora 50 , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Folhas de Planta/química , Ratos , Ratos Sprague-Dawley , Vasos Retinianos/efeitos dos fármacos , Vasos Retinianos/enzimologia , Vasos Retinianos/metabolismo , Peixe-ZebraRESUMO
Previously we found that hypertension potentiates the risk the cataractogenesis. In the present study, we investigated the protective effects of magnesium taurate (MgT) on hypertension and associated lenticular damages against cadmium chloride (CdCl2)-induced hypertensive animals. Male Sprague-Dawley albino rats (150-180g) were assigned to five experimental groups (n=6). Among the five groups, normal group received 0.3% carboxymethyl cellulose (10ml/kg/day, p.o.). Hypertension control group received CdCl2 (0.5mg/kg/day, i.p.). Tests and standard groups received MgT (3 and 6mg/kg/day, p.o.) and amlodipine (3mg/kg/day, p.o.) concurrently with CdCl2 respectively, for six consecutive weeks. Blood pressure, heart rate, and eyes were examined biweekly, and pathophysiological parameters in serum and eye lenses were evaluated after six weeks of the experimental protocol. The chronic administration of MgT concurrently with CdCl2 significantly restored the blood pressure, serum and lens antioxidants (CAT, SOD, GPx, and GSH), MDA level, and ions (Na+, K+, and Ca2+). Additionally, MgT treatment led to significant increase in the lens proteins (total and soluble), Ca2+ ATPase, and Na+K+ ATPase activity as compared to hypertension control group. Ophthalmoscope observations indicated that MgT treatments delayed the progression of cataract against the hypertensive state. The study shows that MgT prevents the progression of cataractogenesis via restoration of blood pressure, lenticular oxidative damages, and lens ATPase functions in the hypertensive state. The results suggest that MgT supplement may play a beneficial role to manage hypertension and associated cataractogenesis.
Assuntos
Adenosina Trifosfatases/metabolismo , Catarata/tratamento farmacológico , Catarata/prevenção & controle , Hipertensão/patologia , Cristalino/enzimologia , Magnésio/uso terapêutico , Estresse Oxidativo , Taurina/uso terapêutico , Animais , Biomarcadores/sangue , Pressão Sanguínea/efeitos dos fármacos , Cloreto de Cádmio , ATPases Transportadoras de Cálcio/metabolismo , Catarata/sangue , Catarata/patologia , Progressão da Doença , Frequência Cardíaca/efeitos dos fármacos , Hipertensão/sangue , Hipertensão/fisiopatologia , Íons , Cristalino/efeitos dos fármacos , Cristalino/patologia , Magnésio/farmacologia , Masculino , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , ATPase Trocadora de Sódio-Potássio/metabolismo , Sístole/efeitos dos fármacos , Taurina/farmacologiaRESUMO
Caffeoylquinic acids, flavonoids, and coumarins isolated from Artemisia capillaris have recently emerged as therapeutic candidates for diabetes and diabetic complications; however, there have been very few studies of the anti-diabetic potential of polyacetylenes. In the present study, we investigated the anti-diabetic potential of two polyacetylenes isolated from A. capillaris, namely capillin and capillinol by investigating their ability to inhibit α-glucosidase, protein tyrosine phosphatase 1B (PTP1B), and rat lens aldose reductase (RLAR). Capillin displayed potent inhibitory activity against α-glucosidase, PTP1B, and RLAR, while capillinol showed moderate inhibitory activity against α-glucosidase and PTP1B at the concentrations tested. In addition, a kinetic study revealed that capillin inhibited α-glucosidase and RLAR in a noncompetitive manner, while inhibited PTP1B in a mixed-type manner. Capillinol inhibited α-glucosidase and PTP1B in a mixed-type manner. Docking simulations of these compounds demonstrated negative binding energies and close proximity to residues in the binding pocket of PTP1B, indicating that these polyacetylenes have a high affinity and tight binding capacity for the active site of the enzyme. Furthermore, capillin dose-dependently inhibited peroxynitrite (ONOO(-))-mediated tyrosine nitration. The results clearly demonstrate the promising potential of capillin and capillinol as therapeutic interventions for the management of diabetes as well as diabetes-associated complications.
Assuntos
Alcinos/farmacologia , Artemisia/química , Di-Inos/farmacologia , Hexanóis/farmacologia , Aldeído Redutase/antagonistas & inibidores , Alcinos/isolamento & purificação , Animais , Di-Inos/isolamento & purificação , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Inibidores de Glicosídeo Hidrolases/farmacologia , Hexanóis/isolamento & purificação , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/farmacologia , Cristalino/enzimologia , Simulação de Acoplamento Molecular , Ácido Peroxinitroso/antagonistas & inibidores , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , RatosRESUMO
Three new phenolic biphenyl derivatives (1-3) and one new lignan glycoside (4) were isolated from the leaves and twigs of Osteomeles schwerinae. The structures of the new compounds were established by spectroscopic data interpretation. The inhibitory effects of 1-4 on rat lens aldose reductase in vitro were examined, and compounds 1-3 markedly inhibited the enzyme with IC50 values of 3.8 to 13.8 µM. In addition, the effects of these isolates on the dilation of hyaloid-retinal vessels induced by high glucose (HG) in zebrafish larvae were investigated. Compound 1 was the most effective in reducing HG-induced dilation of hyaloid-retinal vessels.
Assuntos
Compostos de Bifenilo/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Glicosídeos/isolamento & purificação , Rosaceae/química , Aldeído Redutase/antagonistas & inibidores , Animais , Compostos de Bifenilo/química , Compostos de Bifenilo/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Glucose/análise , Glucose/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Larva/efeitos dos fármacos , Cristalino/enzimologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Folhas de Planta/química , Caules de Planta/química , Ratos , Vasos Retinianos/efeitos dos fármacos , Peixe-Zebra/crescimento & desenvolvimentoRESUMO
This study investigated the urinary and biliary excretion of tectoridin, a major active isoflavonoid found in the flowers of Pueraria thomsonii Benth. and the rhizomes of Belamcanda chinensis (L.) DC. Using UHPLC/Q-TOFMS, seven glucuronides and/or sulfated metabolites and four Phase I metabolites were simultaneously quantified in rat urine after oral administration of tectoridin at 100 and 200 mg/kg. Over a 72-h period, 14.2% and 14.7% of the tectoridin were excreted as eleven metabolites in urine, among which, two major metabolites tectorigenin-7-O-ß-D-glucuronide (Te-7G) and tectorigenin accounted for 5.5-5.5% and 4.3-4.4%. Furthermore, the cumulative excretion of four glucuronides and sulfated metabolites in bile accounted for 7.3% and 3.9% of the dose within 60 h, among which, Te-7G and tectorigenin-7-O-glucuronide-4'-O-sulfate (Te-7G-4'S) accounted for 2.3-3.0% and 1.4-3.9%, respectively. The results indicate that the urine was the primary elimination route, and glucuronidation after deglycosylation at C-7 position was the major metabolic pathway of tectoridin in vivo. Moreover, the inhibitory activities of tectoridin and its five metabolites on rat lens aldose reductase were confirmed (IC50: 1.4-15.5 µM), whereas irisolidone-7-O-glucuronide (Ir-7G) and irisolidone showed little activity.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Isoflavonas/urina , Administração Oral , Animais , Bile/química , Flores/química , Iridaceae/química , Isoflavonas/metabolismo , Isoflavonas/farmacocinética , Cristalino/enzimologia , Masculino , Pueraria/química , Ratos Sprague-Dawley , Rizoma/químicaRESUMO
OBJECTIVE: To study possible inhibition of oxidative stress and cataract formation by single combined formulation of grape seed extract and Zincovit tablets against sodium selenite-induced age-related cataract in Wistar rat pups. METHODS: Oxidative stress and consequent cataract formation was induced by subcutaneous administration of a single dose of sodium selenite (10 µmoles/kg) to Wistar rat pups on day 7 post-natally. In experiments designed to inhibit such cataract formation, the pups were pretreated subcutaneously with combined formulation of grape seed extract and Zincovit tablets (40, 80 and 160 mg/kg), one day prior to the administration of selenite and continuing such treatment till day 20, when the experiments were terminated. The extent of tissue damage caused by the selenite was assessed biochemically by measurements of the levels of reduced glutathione, glutathione peroxidase, glucose-6-phosphate dehydrogenase, protein thiol, catalase, superoxide dismutase, malondialdehyde, aldose reductase, sorbitol dehydrogenase and adenosine triphosphate in the isolated lenses. Cataract formation and its prevention were monitored by examining the eye with pen light illumination and subsequent photography of the isolated lenses. RESULTS: Injection of selenite led to a significant loss of lens clarity due to cataract formation. In the group treated with combined formulation of grape seed extract and Zincovit tablets, the formation of cataract was significantly prevented. In the normal and selenite induced senile cataract control group, the levels of lens oxidative stress markers, G6PD and ATP were substantially lower than in the grape seed extract with Zincovit tablets treated group (p < 0.05). CONCLUSION: Over all, the results suggest that single combined formulation of grape seed extract and Zincovit tablets may offer a prophylactic measure against onset and progression of age- related cataract of human subjects as nutritional food supplement.
Assuntos
Catarata/dietoterapia , Catarata/prevenção & controle , Suplementos Nutricionais , Progressão da Doença , Extrato de Sementes de Uva/farmacologia , Extrato de Sementes de Uva/uso terapêutico , Zinco/farmacologia , Zinco/uso terapêutico , Trifosfato de Adenosina/metabolismo , Animais , Catalase/metabolismo , Catarata/induzido quimicamente , Feminino , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Cristalino/efeitos dos fármacos , Cristalino/enzimologia , Cristalino/metabolismo , Masculino , Malondialdeído/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Selenito de Sódio/administração & dosagem , Superóxido Dismutase/metabolismoRESUMO
DW1029M is a botanical extract consisting of Morus bark and Puerariae radix, produced by Dong-Wha Pharmaceutical, for nephroprotective drug development; it has been in phase II clinical trials in Korea. In our mechanistic investigations, we found that DW1029M inhibits advanced glycation end products (AGEs), rat lens aldose reductase (RLAR), and transforming growth factor (TGF)-ß1 signaling, all of which are implicated in diabetic complications such as diabetic nephropathy and diabetic retinopathy. DW1029M inhibits AGE formation via Fe(2+) chelation. The extract contains 13 active constituents that inhibit AGE formation, 8 active constituents that inhibit RLAR activity, and 1 inhibitor of TGF-ß1 signaling. Our results suggest DW1029M protects against diabetic nephropathy via blockade of AGE formation, RLAR activity, and TGF-ß1 signaling.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Produtos Finais de Glicação Avançada/antagonistas & inibidores , Morus , Extratos Vegetais/farmacologia , Pueraria , Transdução de Sinais/efeitos dos fármacos , Fator de Crescimento Transformador beta1/antagonistas & inibidores , Aldeído Redutase/efeitos dos fármacos , Aldeído Redutase/metabolismo , Animais , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Experimental/metabolismo , Nefropatias Diabéticas/prevenção & controle , Retinopatia Diabética/prevenção & controle , Modelos Animais de Doenças , Feminino , Produtos Finais de Glicação Avançada/efeitos dos fármacos , Produtos Finais de Glicação Avançada/metabolismo , Técnicas In Vitro , Cristalino/enzimologia , Extratos Vegetais/uso terapêutico , Ratos , Ratos Sprague-Dawley , Estreptozocina/efeitos adversos , Fator de Crescimento Transformador beta1/efeitos dos fármacos , Fator de Crescimento Transformador beta1/metabolismoRESUMO
Vicenin 2, isolated from a traditionally used medicinal plant Artemisia capillaris, is a 6,8-di-C-glucoside of apigenin which has been previously reported to possess a wide variety of pharmacological activities including antioxidant, anti-inflammatory, anti-cancer, and hepatoprotective. However, there have not been any reports concerning its anti-diabetic potential until now. Therefore, in the present study, we evaluated the anti-diabetic potential of vicenin 2 via α-glucosidase, protein tyrosine phosphatase 1B (PTP1B), rat lens aldose reductase (RLAR), and advanced glycation end products (AGE) formation inhibitory assays. Vicenin 2 strongly inhibited α-glucosidase, PTP1B, and RLAR in the corresponding assays. In addition, vicenin 2 inhibited the formation of both fluorescent AGE and nonfluorescent AGE, e.g., CML, as well as the level of fructosamine in glucose-fructose-induced bovine serum albumin (BSA) glycation. In the test system, vicenin 2 suppressed glycation-induced protein oxidation by attenuating the formation of protein carbonyl groups as well as by inhibiting the modification of protein thiol groups. Moreover, vicenin 2 was found to be a potent inhibitor of glycation-induced formation of amyloid cross-ß structures in BSA. Taken together, vicenin 2 might be a useful lead for the development of multiple target-oriented therapeutic modalities for the treatment of diabetes and diabetes-associated complications.
Assuntos
Apigenina/isolamento & purificação , Apigenina/farmacologia , Artemisia/química , Inibidores Enzimáticos/farmacologia , Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Produtos Finais de Glicação Avançada/metabolismo , Aldeído Redutase/antagonistas & inibidores , Aldeído Redutase/metabolismo , Amiloide/química , Amiloide/efeitos dos fármacos , Animais , Apigenina/química , Frutosamina/metabolismo , Glucosídeos/química , Produtos Finais de Glicação Avançada/efeitos dos fármacos , Inibidores de Glicosídeo Hidrolases/farmacologia , Cristalino/enzimologia , Lisina/análogos & derivados , Lisina/metabolismo , Oxirredução , Plantas Medicinais/química , Carbonilação Proteica/efeitos dos fármacos , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo , Ratos Sprague-Dawley , Soroalbumina Bovina/química , Soroalbumina Bovina/metabolismo , alfa-Glucosidases/metabolismoRESUMO
Seven new acylated sucroses, mumeoses P-V, were isolated from the flower buds of Prunus mume, cultivated in Zhejiang province, China. Their chemical structures were elucidated on the basis of chemical and physicochemical evidence. Moreover, mumeoses C, D, and R, were shown to substantially inhibit aldose reductase.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Inibidores Enzimáticos/química , Prunus/química , Sacarose/análogos & derivados , Animais , China , Inibidores Enzimáticos/farmacologia , Flores/química , Cristalino/enzimologia , Ratos , Sacarose/química , Sacarose/farmacologiaRESUMO
Diabetic complications are attributed to hyperglycaemic condition which is in turn associated with the polyol pathway and advanced glycation end products. Aldose reductase (AR) is the principal enzyme of polyol pathway which plays a vital role in the development of diabetic complications. AR inhibitory activity can be screened by both in vitro and in vivo methods. In vitro assays for AR enzyme are further classified on the basis of the source of enzyme such as rat lens, rat kidney, cataracted human eye lens, bovine eyes and human recombinant AR enzymes, whereas the in vivo model is based on the determination of lens galactitol levels. A number of synthetic AR inhibitors (ARIs) including tolrestat and sorbinil have been developed, but all of these suffer from drawbacks such as poor permeation and safety issues. Therefore, pharmaceutical companies and many researchers have been carrying out research to find new, potent and safe ARIs from natural sources. Thus, many naturally occurring compounds have been reported to have AR inhibitory activity. The present review attempts to highlight phytochemicals and plant extracts with potential AR inhibitory activity. It also summarizes the classes of compounds which have proven AR inhibitory activity. Phytochemicals such as quercetin, kaempferol and ellagic acid are found to be the most promising ARIs. The exhaustive literature presented in this article clearly indicates the role of plant extracts and phytochemicals as potential ARIs.
Assuntos
Aldeído Redutase/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Animais , Bovinos , Ácido Elágico/farmacologia , Inibidores Enzimáticos/química , Galactitol/metabolismo , Humanos , Hiperglicemia/tratamento farmacológico , Quempferóis/farmacologia , Cristalino/enzimologia , Compostos Fitoquímicos/química , Extratos Vegetais/química , Quercetina/farmacologia , RatosRESUMO
PURPOSE: Accumulating evidence suggests that inhibitors of aldose reductase (AR) may prevent hyperglycemia-induced long-term complications in diabetes mellitus. In the present study, we evaluated the AR inhibitory potential of ethanolic (EtOH) extracts from 22 seaweed species. METHODS: AR inhibitory activities of the selected seaweed species were evaluated using the rat lens aldose reductase (RLAR) inhibitory assay. RESULTS: All extracts exhibited RLAR inhibitory activity, which ranged from 5.87 to 92.71 % at a concentration of 50 µg/mL. Since Capsosiphon fulvescens exhibited significant inhibitory potential and is a frequently used foodstuff, it was selected for a detailed investigation using RLAR and advanced glycation end products (AGE) formation inhibitory assays. Among the different solvent-soluble fractions, the CH2Cl2, EtOAc, and n-BuOH fractions showed promising RLAR and AGE formation inhibitory activities. Considering the AR inhibitory potential, CH2Cl2 and EtOAc fractions were selected for chromatographic separation and yielded 11 compounds in which capsofulvesin A, capsofulvesin B, and chalinasterol showed potential RLAR inhibitory activity with the respective IC50 values of 52.53, 101.92, and 345.27 µM. Kinetic studies revealed that capsofulvesin A and chalinasterol exhibited mixed type inhibition, while capsofulvesin B exhibited noncompetitive inhibition. To our knowledge, this is the first report of AR inhibitory activity of the glycolipids capsofulvesin A and capsofulvesin B. CONCLUSIONS: Our results clearly indicate the potential RLAR and AGE formation inhibitory activities of C. fulvescens as well as its isolated constituents, which could be further explored to develop therapeutic modalities for the treatment of diabetes and related complications.