The effects of Cynodon dactylon (Poaceae family) and Dolichos biflorus (Fabaceae family) extracts on decreasing size and excretion of kidney and urinary tract stones: a randomized, double-blind controlled trial.

OBJECTIVES: Given high and growing prevalence rate of urolithiasis in most societies as well as the problems caused by this issue, it is necessary to apply more cost-effective and safer therapeutic methods, which are accessible for all the individuals worldwide. Therefore, this study aimed to investigate efficacy of herbal medicines named Cynodon dactylon and Dolichos biflorus on solving and excretion of renal and urinary tract stones in patients with urolithiasis. METHODS: This study included 96 patients with urolithiasis who were randomly allocated into three groups. The first group received the extract of D. biflorus seeds (1,600 mg), the second group received extract of C. dactylon rhizome (1,600 mg) and the third group received placebo for 21 days. In this study, we used an hydroalcoholic extract of D. biflorus and C. dactylon prepared by Soxhlet method. For each patient, the size of the stones, the amount of calcium in the urine, the number of stones excreted and their chemical substance type were measured. RESULTS: In this study, changes were observed at the significance level in the interventions groups of 1 and 2, and the placebo group in the left kidney, so that changes in size of the stone in left kidney as intergroup were significantly different in these three groups (p=0.02). The mean of changes in stone size in left kidney in the group C. dactylon was 3.78 ± 7.1 and in the group D. biflorus, it was 0.27 ± 0.6. CONCLUSIONS: A significant difference in the results of this study show that C. dactylon rhizome and D. biflorus seed extracts are able to decrease the size of the stone and can be effective on kidney stones excretion.

Pharmacological properties of durva swaras (Cynodon dactylon L. Pers.) in an ovariectomised rat model mimicking chronic menopausal syndrome.

Hormonal replacement therapy (HRT), as the first-line management of chronic menopausal syndrome (CMS) in women, has limited application due to adverse effects. We aimed to evaluate the therapeutic potential of a herbal alternative (HALT), durva swaras (DS) of Cynodon dactylon L. Pers., in a CMS rat model. Female Sprague-Dawley rats were subjected to Sham and ovariectomy (OVX) surgery. OVX rats received either 0.11 mg/kg oestrogen as a positive treatment control or 1 (DS1), 2 (DS2), and 4 (DS3) g/kg DS for 160 days. Vaginal smear tests indicated the menopausal status. Routine clinical examinations, weekly body weights (BW), serum calcium, proinflammatory cytokines, and reproductive hormones levels were monitored. Clinical chemistry, body composition, bone mineral density (BMD), uterotrophic response, bone morphometry, and histopathology of major organs were evaluated. BW of OVX rats increased by 18-25% compared to Sham. Total fat and fat percentage were significantly elevated in the oestrogen group compared to DS2, DS3, and OVX group. DS treatment groups showed the levels of TNF- α was slightly reduced, while IL-1ß and IL-6 levels were significantly reduced (P < 0.05) compared to the oestrogen treated group. DS treatment restored serum calcium levels, while BMD, bone quality, osteoblast/osteoclast ratio, and collagen levels improved in both DS and oestrogen treatment groups. The uterotrophic assay demonstrated non-oestrogenic activity of DS. Endometrial hyperplastic change was observed in oestrogen-treated rats. The preclinical non-oestrogenic activity of DS has therapeutic potential in CMS through anti-inflammatory and osteo-protective effects. Further clinical research into DS, as a viable HALT to HRT, is required.

Bioengineered gold nanoparticles using Cynodon dactylon extract and its cytotoxicity and antibacterial activities.

In this study, simple and green route approach was applied for the synthesis gold nanoparticles (AuNPs) containing an aqueous extract of Cynodon dactylon L. Pers., (C. dactylon). The synthesized AuNPs were characterized using spectral and microscopic analysis. The changes in the color pattern were observed upon synthesis by UV-vis spectrophotometer with a peak of 530 nm. The FT-IR, XRD, SEM, and TEM were used to analyze the crystal nature and morphology of the green synthesized AuNPs. The C. dactylon-loaded AuNPs in different concentrations (0.625-100 µg/ml) were used to assess cytotoxicity activity against MCF-7 cell line and where the IC50 was found to be 31.34 µg/ml by MTT assay. The C. dactylon-AuNPs were significantly increased reactive oxygen species (ROS) generation, DNA fragmentation, and mitochondrial membrane changes observed by dichlorodihydroflurescenin diacetate (DCFH-DA), 4',6-diamidino-2-phenylindole (DAPI), Rhodamine-123, and acridine orange (AO)/ethidium bromide (EtBr) staining assay. Besides the microbial study revealed that C. dactylon-AuNPs exhibited significant antibacterial activity against clinically isolated pathogenic bacteria such as Enterobacter cloacae, Staphylococus Haemolytics, Staphylococcus petrasii subsp. Pragensis and Bacillus cereus with a zone of inhibition 13, 12, 13 and 12 mm, respectively. It could be concluded that C. dactylon has the ability to be involved in the biosynthesis of AuNPs, and the pharmacological studies proved the promising cytotoxic effect on MCF-7 cell line and pathogenic bacterial species.

Effective methane production from the Japanese weed Gyougi-shiba (Cynodon dactylon) is accomplished by colocalization of microbial communities that assimilate water-soluble and -insoluble fractions.

Weed, an abundant biomass, is considered unsuitable as a raw material for methane production. There are few reports on the anaerobic digestion of weeds without the addition of other organic wastes. To solve this problem, a methane-producing microbial community with weed as a sole feedstock was established. This study mainly focused on the degree of contribution between water-soluble and -insoluble fractions of the weed to methane production; thus, methane production from both fractions was tested separately. Methane production after 80-day batch cultures with whole weed, water-soluble and water-insoluble fractions was 184.5, 96.8 and 26.5 NmL g-1 dry matter (DM), respectively. The results of 16S rRNA gene amplicon sequence analysis revealed that Proteiniphilum saccharofermentans and several Methanobacterium species commonly dominated all cultures, whereas the population dynamics of minor species differed in every culture. Moreover, the remixed culture of microbial communities adapted to water-soluble and -insoluble fractions recovered methane production (252.4 NmL g-1 DM). Based on these results, it can be strongly inferred that colocalizing the minor species in water-soluble and -insoluble fractions is important for effective methane production.

Cysestermerol A, a rare stilbene sestermer with significant hypoglycemic activities from Cynodon dactylon.

Cysestermerol A (1), a rare and new stilbene sestermer, was isolated from the whole herb of Cynodon dactylon. The planar and relative structures of 1 were elucidated based on HRESIMS, one- and two-dimensional NMR analyses, and its absolute configuration was further established by electronic circular dichroism calculations. Compound 1 obviously increased the glucose consumption in HepG2 cells equivalent to the positive control rosiglitazone and markedly inhibited the activity of α-glucosidase in vitro.

Integration of Cynodon dactylon and Muraya koenigii plant extracts in amino-functionalised silica-coated magnetic nanoparticle as an effective sorbent for the removal of chromium(VI) metal pollutants.

Immobilised magnetic nanoparticles are extensively used owing to their superparamagnetic nature, surface interaction, and binding specificity with the appropriate portentous substances. The present research focuses on the development of a portentous, robust carrier, which integrates the silica-coated amino-functionalised magnetic nanoparticle (AF-MnP) with the plant extracts of Cynodon dactylon (L1) and Muraya koenigii (L2) for the stable and enhanced removal of hazardous hexavalent chromium pollutant in the wastewater. Vibrating sample magnetometer (Ms - 45 emu/g) determines the superparamagnetic properties; Fourier-transform infrared spectroscopy determines the presence of functional groups such as NH2, Si-O-Si, C=C; high-resolution transmission electron microscopy, field emission scanning electron microscope and energy-dispersive X-ray spectroscopy determine the size of the green adsorbents in the range of 20 nm and the presence of elements such as Fe, N, and Si determines the efficacy of the synthesised silica-coated AF-MnP. The AF-MnP-L1 shows the maximum adsorption capacity of 34.7 mg/g of sorbent calculated from the Langmuir isotherm model and the process follows pseudo-second-order kinetics. After treatment, the adsorbents can be easily separated from the solution in the presence of an external magnetic field and are reused for nine cycles after acid treatment with the minimal loss of adsorption efficiency.

The importance of nutrient hotspots for grazing ungulates in a Miombo ecosystem, Tanzania.

While movement patterns of grazing ungulates are strongly dependent on forage quality their use of nutrient hotspots such as termite mounds or grazing lawns has rarely been quantified, especially in savanna ecosystems where soil-nutrient quality is low. Additionally, few experiments have been conducted to determine the role of termite mound- and grazing lawn-derived soils in improving forage quality in the field. We studied wild ungulate grazing activities around ten termite mounds, six grazing lawns and their respective control sites in a Miombo system of Issa Valley, western Tanzania, in the same system. We used indirect observations (i.e., dung, tracks) to identify seasonal and spatial variations in habitat use of various wild mammalian grazers. Grazer visitation rates were nine and three times higher on termite mounds and grazing lawns, respectively, compared to control sites. During the rainy season, termite mounds were more frequently used than grazing lawns while the latter were used more often during the dry season. In an additional pot experiment with soils derived from different areas, we found that Cynodon dactylon in termite mound-derived soils had twice as high Nitrogen and Phosphorous contents and biomass compared to grasses planted in grazing lawn soils and control site soils. We highlight that both termite mounds and grazing lawns play a significant role in influencing seasonal nutrient dynamics, forage nutrient quality, habitat selectivity, and, hence, grazing activities and movement patterns of wild ungulate grazers in savannas. We conclude that termite mounds and grazing lawns are important for habitat heterogeneity in otherwise nutrient-poor savanna systems.

Efficient removal of aqueous hexavalent chromium by activated carbon derived from Bermuda grass.

In this study, a novel raw material, Bermuda grass had been devised for the synthesis of activated carbon (BGAC) and enhanced the pore volume by potassium hydroxide. The effects of different factors on activated carbon products by orthogonal experiment was optimized. The synthesized BGAC was characterized by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, energy dispersive X-ray (EDX), X-ray photoelectron spectroscopy (XPS) and then, Cr(VI) removal batch experiments were conducted to investigate the Cr(VI) removal performance. Kinetic model and Weber-Morris diffusion model were fitted to the Cr(VI) removal process indicated that the chemisorption was the predominant removal mechanism and intraparticle diffusion was the sole rate-controlling mechanism. Langmuir isotherms could fit the experimental date well, which revealed that the adsorption of Cr(VI) ions was monolayer adsorption and the maximum adsorption capacity could be reached at 403.23 mg g-1. The results also promulgated that BGAC had an excellent potential on Cr(VI) removal. The removal processes were considered to comprise adsorption, reduction, precipitation and other ways through the study of the removal mechanism.

137Cs and 40K concentration ratios (CRs) in annual and perennial plants in the Caspian coast.

This research attempted to investigate the plant/soil concentration ratios (CRs) of 137Cs and 40K in plants that grow on the Anzali Lagoon of the Caspian coast, Iran. The activity concentrations of 137Cs and 40K were measured in soil samples, annual plants (Echinochloa crus-galli, Digitaria sanguinalis, and Trifolium repens), and perennial plants (Phragmites australis, and Cynodon dactylon). The relationship of activity concentration with soil particle density, and pH of soil samples, CR, and 137Cs/40K discrimination factor (DF) were determined. The activity concentration of 137Cs in the soil, annual plants, and perennial plants ranged between 12- and 124 (Bq/kg dry weight), 2- and 17 (Bq/kg dw), and 2 - and 14 (Bq/kg dw), respectively. The geometric mean (GM) values of DF in annual and perennial plants were 0.57 and 0.55, respectively. The CR for 137Cs and 40K varied as a non-linear relation in annual plants and perennial plants. This study showed that the CRs for 137Cs in annual plants were higher than the CRs for perennial plants.

Exogenous fibrolytic enzymes and recombinant bacterial expansins synergistically improve hydrolysis and in vitro digestibility of bermudagrass haylage.

Four experiments were conducted to examine the effects of a recombinant bacterial expansin-like protein (BsEXLX1) from Bacillus subtilis and a commercial exogenous fibrolytic enzyme (EFE) preparation for ruminants on hydrolysis of pure substrates (cellulose and xylan) and in vitro digestibility of bermudagrass haylage (BMH). Recombinant Escherichia coli BL21 strain was used to express BsEXLX1; the protein was purified using an affinity column. In experiment 1, carboxymethylcellulose, Whatman #1 filter paper (General Electric, Boston, MA) and oat-spelt xylan substrates were subjected to 4 treatments (1) sodium citrate buffer (control), (2) BsEXLX1 (162 µg/g of substrate), (3) EFE (2.3 mg/g of substrate), and (4) EFE + BsELX1 in 3 independent runs. Samples were incubated at optimal conditions for both additives (pH 5 and 50°C) or at ruminal (pH 6 and 39°C) or ambient (pH 6 and 25°C) conditions for 24 h and sugar release was measured. In experiment 2, digestibility in vitro of BMH was examined after treatment with the following: (1) control (buffer only), (2) BsEXLX1 (162 µg/g of dry matter), (3) EFE (2.2 mg/g of dry matter), and (4) EFE + BsEXLX1 in 3 independent runs at 39°C for 24 h. Experiment 3 examined effects of EFE and BsEXLX1 on simulated preingestive hydrolysis and profile of released sugars from BMH after samples were suspended in deionized water with sodium azide at 25°C for 24 h in 2 independent runs. In experiment 4, the sequence of the BsEXLX1 purified protein was compared with 447 ruminal bacterial genomes to identify similar proteins from the rumen. In experiment 1, compared with EFE alone, EFE and BsEXLX1 synergistically increased sugar release from carboxymethylcellulose and Whatman #1 filter paper under all simulated conditions; however, hydrolysis of xylan was not improved. In experiment 2, compared with EFE alone, treatment with EFE and BsEXLX1 increased neutral detergent fiber and acid detergent fiber digestibility of bermudagrass haylage (by 5.5 and 15%, respectively) and total volatile fatty acid concentrations, and decreased acetate-propionate ratio. In experiment 3, compared with EFE alone. The EFE and BsEXLX1 synergistically reduced concentrations of neutral detergent fiber and acid detergent fiber and increased release of sugars by 9.3%, particularly cellobiose (72.5%). In experiment 4, a similar sequence to that of BsEXLX1 was identified in Bacillus licheniformis, and similar hypothetical protein sequences were identified in Ruminococcus flavefaciens strains along with different protein structures in E. xylanophilum and Lachnospiraceae. This study showed that an expansin-like protein synergistically increased the hydrolysis of pure cellulose substrates and the hydrolysis and digestibility in vitro of BMH.

Effect of dietary supplementation with phytogenic blend on growth performance, apparent ileal digestibility of nutrients, intestinal morphology, and cecal microflora of broiler chickens.

The effects of dietary supplementation with phytogenic blend (PB) of Aerva lanata, Piper betle, Cynodon dactylon, and Piper nigrum on growth performance, ileal nutrient digestibility, intestinal morphology, and cecal microflora were determined in a 42-day broiler feeding trial. A total of 192 broilers were assigned to 4 dietary treatments (6 replicates and 8 birds/replicate): basal diet, basal diet supplemented with antibiotic (chlortetracycline), 1% and 2% PB, respectively. The body weight gain (BWG) of starter chicks increased linearly (P = 0.023) as dietary supplementation levels of PB increased. At grower phase, broilers fed diet supplemented with 1% PB had similar BWG with the antibiotic group, but other treatments had reduced (P = 0.0001) BWG. Dietary supplementation with 1% PB resulted in the highest (P < 0.0001) BWG during the study. Feed intake was not affected by the treatments during the starter, finisher, and overall rearing periods. Broilers fed diet supplemented with 1% PB had the best (P < 0.0001) feed conversion ratio during the study. Overall, broilers fed only basal diet had the highest (P = 0.0450) mortality. Ileal organic matter (OM) digestibility increased linearly (P = 0.044) with broilers fed diet supplemented with PB, but reduced with antibiotic group. Dietary supplementation with 1% PB had the highest (P = 0.0402) ileal digestibility of tryptophan. In the duodenum, broilers fed diet supplemented with PB had longer (P = 0.0006) villi heights than the birds fed only basal diet, but similar with antibiotic group. Broilers fed diet supplemented with PB had longer (P = 0.0064) villi height in the jejunum than the antibiotic group. Bifidobacterium concentration of the cecum content showed a slight increase (P = 0.053) with increasing supplementation levels of PB. In conclusion, the current study shows that dietary supplementation with PB improves growth performance, intestinal morphology, and apparent ileal digestibility of OM and tryptophan in a dose-dependent manner with the best response at 1% inclusion level.

Collagen-silica bio-composite enriched with Cynodon dactylon extract for tissue repair and regeneration.

Development of biomaterials for tissue engineering applications is of great interest to meet the demand of different clinical requirements. The wound heal dressing biomaterials should necessarily contain well-defined therapeutic components and desirable physical, chemical and biological properties to support optimal delivery of therapeutics at the site of the wound. In this study, we developed collagen-silica wound heal scaffold incorporated with the extract of Cynodon dactylon, characterized and evaluated for its wound heal potential in vitro and in vivo against collagen (Col) and Collagen-silica (CS) scaffolds that served as controls. The prepared Collagen-Silica-Cynodon extract (CSCE) scaffold exhibits porous morphology with preferable biophysical, chemical, mechanical and mass transfer properties besides its controlled biodegradation at the wound site. Stability of CSCE was found to be better than that of native collagen due to intermolecular interactions between collagen and constituents of C. dactylon as confirmed by FTIR analysis. Notably, in vitro biocompatibility assay using DAPI and Rhodamine 123 staining demonstrated that the proliferation of NIH3T3 fibroblast cells was better for CSCE when compared to the Col and CS scaffolds. In vivo wound healing experiments with full-thickness excision wounds in wistar rat model demonstrated that the wounds treated with CSCE showed accelerated healing with enhanced collagen deposition when compared to wounds treated with Col and CS scaffolds, and these studies substantiated the efficacy of CSCE scaffold for treating wounds.

Replacement of Tifton hay by spineless cactus in Girolando post-weaned heifers´ diets.

This study aims to evaluate the effect of the Tifton 85 hay (Cynodon dactylon L. Pers) replacement by Orelha de Elefante Mexicana spineless cactus (Opuntia stricta [Haw.] Haw) on the nutrient intake and digestibility, growth performance, microbial protein synthesis, and efficiency of dietary nitrogen compound utilization in the diets of Girolando heifers. Twenty-four (3.5 months and 100 kg ± 3.5 kg) Girolando heifers (5/8 Holstein x Gyr) were used and arranged in a completely randomized design. Dry matter, organic matter, crude protein, and neutral detergent fiber decreased linearly as a function of the levels of Tifton hay (TH) replacement by Orelha de Elefante Mexicana spineless cactus (OEM). The non-fiber carbohydrate intake and coefficient of dry matter digestibility increased, while the neutral detergent fiber (NDF) digestibility decreased linearly with the OEM inclusion. After evaluating the growth performance by observing weight gain and morphometric measurements, there were no significant alterations. The urinary volume, nitrogen balance as well as the efficiency of nitrogen retention had increased, while the urinary and plasma urea concentrations had decreased linearly with the OEM inclusion. The results obtained in this study show that Orelha de Elefante Mexicana spineless cactus should replace Tifton hay in post-weaned heifer diets.

Role of sodium ion transporters and osmotic adjustments in stress alleviation of Cynodon dactylon under NaCl treatment: a parallel investigation with rice.

Comparative analyses of the responses to NaCl in Cynodon dactylon and a sensitive crop species like rice could effectively unravel the salt tolerance mechanism in the former. C. dactylon, a wild perennial chloridoid grass having a wide range of ecological distribution is generally adaptable to varying degrees of salinity stress. The role of salt exclusion mechanism present exclusively in the wild grass was one of the major factors contributing to its tolerance. Salt exclusion was found to be induced at 4 days when the plants were treated with a minimum conc. of 200 mM NaCl. The structural peculiarities of the salt exuding glands were elucidated by the SEM and TEM studies, which clearly revealed the presence of a bicellular salt gland actively functioning under NaCl stress to remove the excess amount of Na+ ion from the mesophyll tissues. Moreover, the intracellular effect of NaCl on the photosynthetic apparatus was found to be lower in C. dactylon in comparison to rice; at the same time, the vacuolization process increased in the former. Accumulation of osmolytes like proline and glycine betaine also increased significantly in C. dactylon with a concurrent check on the H2O2 levels, electrolyte leakage and membrane lipid peroxidation. This accounted for the proper functioning of the Na+ ion transporters in the salt glands and also in the vacuoles for the exudation and loading of excess salts, respectively, to maintain the osmotic balance of the protoplasm. In real-time PCR analyses, CdSOS1 expression was found to increase by 2.5- and 5-fold, respectively, and CdNHX expression increased by 1.5- and 2-fold, respectively, in plants subjected to 100 and 200 mM NaCl treatment for 72 h. Thus, the comparative analyses of the expression pattern of the plasma membrane and tonoplast Na+ ion transporters, SOS1 and NHX in both the plants revealed the significant role of these two ion transporters in conferring salinity tolerance in Cynodon.

Valor nutritivo de gramíneas do gênero Cynodon consorciadas com amendoim forrageiro / Nutritive value of grasses of genus Cynodon mixed with forage peanut

O objetivo do presente estudo foi avaliar o valor nutritivo do consórcio forrageiro entre gramíneas do gênero Cynodon (cv. tifton 85 ou coastcross) com Arachis pintoi (amendoim forrageiro). O experimento foi conduzido na Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, em área de aproximadamente 3000m2. O delineamento experimental foi de blocos ao acaso, com quatro tratamentos e três repetições. Os tratamentos foram: tifton 85 e coastcross em cultivo estreme, tifton 85 + AF e coastcross + AF. Foram avaliados os teores de PB, FDN, FDA, DIVMS, MM e MS das lâminas foliares, colmo+bainha, amendoim forrageiro e da MF disponível da simulação de pastejo. Foi observado que a coastcross tem menor teor de fibra, independentemente do tratamento. O avanço das estações do ano proporcionou menores valores de PB e DIVMS em todos os tratamentos. A simulação de pastejo demonstrou que a tifton 85 tem maiores teores de MS, assim como valores inferiores são encontrados para os consórcios. Quando consorciado com as gramíneas, o amendoim forrageiro reduziu os compostos fibrosos na pastagem, acrescentando maiores teores de PB em pastagem de gramíneas no final do ciclo produtivo.(AU)

The goal of this study was to evaluate the fodder performance and production of Cynodon (Cv. Tifton 85 or Coastcross) mixed with Arachis pintoi (Forage peanut). The experiment was conducted at the Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, at an area of approximately 3000m2. A completely randomized block design was applied, with four treatments and three replicates. The treatments were: Tifton 85 and Coastcross in pure crop, Tifton 85 + AF and Coastcross + AF with rotated and successive implantation of one line to the grass and three consecutive lines to the legume. The content of CP, NDF, ADF, IVDMD ashe and DM of the leaf blades, stem of grasses forage peanut and total forage mass available of grazing simulation was evaluated. Coastcross has lower fibrous contents, regardless of treatment. The changing of the seasons during the year provided lower values of CP and IVDMD for all treatments. The evaluation of the grazing simulation showed that Tifton 85 has higher DM values just as the lower ones are found for consortia. When mixed with grasses, forage peanuts reduce the fibrous compounds on pasture, increasing the CP levels in grass pasture at the end of productive cycle.(AU)

Tensile strength of warm and cool season forage grasses in Florida.

The tensile strength (TS) of four warm-season and three cool-season forage grasses was measured with an Instron Universal machine, along with cell-wall analysis and determination of in vitro organic matter digestibility. The mean TS of the warm-season grasses was significantly higher than that of the cool season grasses (22 vs. 9 kg, respectively, p < .05). The concentrations of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) were significantly greater in the warm- than in the cool-season grasses (p < .05), whereas ash concentration was greater in the cool-season grasses. Among the warm-season grasses that were studied, elephant grass had the highest and bermuda grass had the lowest TS (34.4 and 14.9 kg, respectively, p < .05); Among the cool-season grasses triticale had greater TS than wheat and oats (12.6 vs. 6.8 and 7.5 kg, respectively, p < .05). TS was significantly correlated with NDF, ADF, and ADL, and negatively correlated with in vitro organic matter digestibility (correlation coefficients were 0.64, 0.73. 0.41, and -0.64, respectively). PRACTICAL APPLICATIONS: Grass tensile strength may have implications on animal preference and on the energy that animals must spend during grazing, and consequently on animal performance (feed intake, daily weight gain and milk, and meat production). Information on grass TS would help to select and screen improved forage cultivars and enable to improve grassland management with better animal performance.

Two new stilbene trimers from Cynodon dactylon.

Many naturally occurring oligostilbenes have drawn considerable attention because of their intricate structures and diverse bioactivities. Two new stilbene trimers, cystibenetrimerol A (1) and cystibenetrimerol B (2) were isolated from the dried grass of Cynodon dactylon (L.) Pers. The planar structures and stereo configurations of them were elucidated by spectroscopic and spectrometric methods. The isolation and structures elucidation of two new stilbene trimers suggested the ordinary grass belonging to the family Poaceae may be a rich source of stilbene oligomers.

Effects of selenium biofortification of hayfields on measures of selenium status in cows and calves consuming these forages.

Two experiments were conducted to evaluate the Se status of weaned calves (Exp. 1) and pregnant cows and newborn calves (Exp. 2) consuming bermudagrass [ (L.) Pers.] fertilized with Se. Sodium selenate was dissolved into water (8.8 g/L) and sprayed onto hayfields. Selenium-fertilized forage had greater ( ≤ 0.001) Se concentration compared with the control forage without Se fertilization (7.7 ± 1.81 vs. 0.1 ± 0.04 mg Se/kg DM and 10.8 vs. 0.1 mg Se/kg DM for Exp. 1 and Exp. 2, respectively). In Exp. 1, beef calves ( = 32; 176 ± 8.7 kg initial BW) were stratified by BW and randomly assigned to pens (16 pens; 2 calves/pen). Treatments were randomly assigned to pens, including control (no supplemental Se), Se hay, or sodium selenite ( = 2, 7, and 7 pens, respectively). A 42-d pair-feeding design was used, wherein each pen receiving Se hay was paired to a pen receiving sodium selenite. Blood and liver samples were collected on d 0, 21, and 42. Liver Se concentrations were greatest ( ≤ 0.005) on d 42 for calves provided Se hay compared with calves provided sodium selenite or control. This difference was attributed only to paired-feeding groups consuming <3 mg Se daily. In Exp. 2, mature, late-pregnancy cows were randomly assigned to 3 treatments: Se hay ( = 12), sodium selenite ( = 9), or control (no supplemental Se; = 6). Cows assigned to the sodium selenite and Se hay treatments were provided 2.5 mg of supplemental Se daily. Upon enrollment, cows were moved into individual feeding areas at an estimated 30 d prior to calving. Following calving, cotyledon and colostrum samples were collected from cows. Four days later, liver and blood samples were collected from both cows and calves. Selenium-supplemented cows had greater ( ≤ 0.001) liver, cotyledon, plasma, and whole blood Se concentrations compared with cows not receiving supplemental Se. Furthermore, cows provided Se hay tended ( = 0.11) to have greater liver Se concentrations compared with cows provided sodium selenite. Calves born to Se-supplemented cows had greater ( = 0.001) plasma Se concentrations than calves born to cows receiving no supplemental Se. Furthermore, calves born to cows provided Se hay tended ( = 0.06) to have greater plasma Se concentrations compared with calves born to cows provided sodium selenite. These data imply that Se biofortification of hayfields is an effective method to increase Se concentration of forage. Consumption of these forages result in increased Se status of weaned calves, periparturient cows, and their calves.

Toxicity of iron oxide nanoparticles to grass litter decomposition in a sandy soil.

We examined time-dependent effect of iron oxide nanoparticles (IONPs) at a rate of 2000 mg kg-1 soil on Cynodon dactylon litter (3 g kg-1) decomposition in an arid sandy soil. Overall, heterotrophic cultivable bacterial and fungal colonies, and microbial biomass carbon were significantly decreased in litter-amended soil by the application of nanoparticles after 90 and 180 days of incubation. Time dependent effect of nanoparticles was significant for microbial biomass in litter-amended soil where nanoparticles decreased this variable from 27% after 90 days to 49% after 180 days. IONPs decreased CO2 emission by 28 and 30% from litter-amended soil after 90 and 180 days, respectively. These observations indicated that time-dependent effect was not significant on grass-litter carbon mineralization efficiency. Alternatively, nanoparticles application significantly reduced mineral nitrogen content in litter-amended soil in both time intervals. Therefore, nitrogen mineralization efficiency was decreased to 60% after 180 days compared to that after 90 days in nanoparticles grass-litter amended soil. These effects can be explained by the presence of labile Fe in microbial biomass after 180 days in nanoparticles amendment. Hence, our results suggest that toxicity of IONPs to soil functioning should consider before recommending their use in agro-ecosystems.

Evaluation of Cynodon dactylon for wound healing activity.

BACKGROUND AND AIMS: Research in the field of wound healing is very recent. The concept of wound healing is changing from day to day. Ayurveda is the richest source of plant drugs for management of wounds and Cynodon dactylon L. is one such. The plant is used as hemostatic and wound healing agent from ethnopharmacological point of view. Aim of the present study is scientific validation of the plant for wound healing activity in detail. MATERIALS AND METHODS: Aqueous extract of the plant was prepared and phytochemical constituents were detected by HPLC analysis. Acute and dermatological toxicity study of the extract was performed. Pharmacological testing of 15% ointment (w/w) of the extract with respect to placebo control and standard comparator framycetin were done on full thickness punch wound in Wister rats and effects were evaluated based on parameters like wound contraction size (mm2), tensile strength (g); tissue DNA, RNA, protein, hydroxyproline and histological examination. The ointment was applied on selected clinical cases of chronic and complicated wounds and efficacy was evaluated on basis of scoring on granulation, epithelialization, vascularity as well as routine hematological investigations. RESULTS: Significant results (p<0.05) were observed both in pharmacological and clinical studies. CONCLUSION: The present research with aqueous extract of Cynodon dactylon explores its potential wound healing activity in animal model and subsequent feasibility in human subjects. Phenolic acids and flavonoids present in c. dactylon supports its wound healing property for its anti-oxidative activity that are responsible for collagenesis.