Biomolecular analyses enable new insights into ancient Egyptian embalming.

The ability of the ancient Egyptians to preserve the human body through embalming has not only fascinated people since antiquity, but also has always raised the question of how this outstanding chemical and ritual process was practically achieved. Here we integrate archaeological, philological and organic residue analyses, shedding new light on the practice and economy of embalming in ancient Egypt. We analysed the organic contents of 31 ceramic vessels recovered from a 26th Dynasty embalming workshop at Saqqara1,2. These vessels were labelled according to their content and/or use, enabling us to correlate organic substances with their Egyptian names and specific embalming practices. We identified specific mixtures of fragrant or antiseptic oils, tars and resins that were used to embalm the head and treat the wrappings using gas chromatography-mass spectrometry analyses. Our study of the Saqqara workshop extends interpretations from a micro-level analysis highlighting the socio-economic status of a tomb owner3-7 to macro-level interpretations of the society. The identification of non-local organic substances enables the reconstruction of trade networks that provided ancient Egyptian embalmers with the substances required for mummification. This extensive demand for foreign products promoted trade both within the Mediterranean8-10 (for example, Pistacia and conifer by-products) and with tropical forest regions (for example, dammar and elemi). Additionally, we show that at Saqqara, antiu and sefet-well known from ancient texts and usually translated as 'myrrh' or 'incense'11-13 and 'a sacred oil'13,14-refer to a coniferous oils-or-tars-based mixture and an unguent with plant additives, respectively.

Non-Invasive Paleo-Metabolomics and Paleo-Proteomics Analyses Reveal the Complex Funerary Treatment of the Early 18th Dynasty Dignitary NEBIRI (QV30).

Biochemical investigations were carried out on the embalmed head of Nebiri (Museo Egizio, Turin; S-5109)-an 18th Dynasty Ancient Egyptian dignitary-and on the canopic jar containing his lungs (Museo Egizio, Turin; S. 5111/02) with the aim of characterizing the organ's (lung) specific paleo-proteins and of identifying the compounds used in his embalming "recipe". The application of a functionalized film method allowed us to perform a non-invasive sampling. Paleo-proteomics confirmed the presence of lung tissue-specific proteins (organ specific) as well as the presence of proteins linked to severe inflammation. Paleoproteomics and paleometabolomics further allowed the identification of the main components of Nebiri's embalming recipe: animal fats and glue, balms, essential oils, aromatic plants, heated Pistacia, and coniferous resins. Both the use of Pistacia and coniferous resins in an early 18th Dynasty individual confirm Nebiri's high social status. The technique applied offers a targeted approach to the chemical characterization of human tissues, embalming compounds, and organic materials layering in pottery. The ability of the functionalized film method to harvest all types of compounds, from macromolecules (i.e., proteins) to small molecules (i.e., organic acids) opens a new path in the study of ancient material culture; furthermore, it allows to perform untargeted analysis, which is necessary when no a priori information is available.

Rethinking the Process of Animal Mummification in Ancient Egypt: Molecular Characterization of Embalming Material and the Use of Brassicaceae Seed Oil in the Mummification of Gazelle Mummies from Kom Mereh, Egypt.

The study of animal mummification in ancient Egypt has recently received increasing attention from a number of modern scholars given the fact that this part of ancient Egyptian funerary and religious history is a practice yet to be fully understood. In this study, nine samples of embalming matter were extracted from six gazelle mummies from the archaeological site of Kom Mereh (modern village of Komir), dated to the Roman period of dominance in ancient Egypt. All samples were analyzed for the presence of inorganic and organic matter applying a multi-analytical approach based on Fourier transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS). Furthermore, in order to identify more specific compounds such as bitumen and beeswax in studied balms, each sample was subjected to a solid phase extraction (SPE) and saponification separation process, respectively. The results of this study revealed that the majority of the analyzed embalming substances sampled from six gazelle mummies from Kom Mereh were complex mixtures of plant oils, animal fats, conifer resin, and beeswax. In this regard, this study was able to report a practice until now unmentioned in the scientific literature, namely, the use of cruciferous oil, derived from seeds of Brassicaceae plants, in animal mummification.

Comprehensive two-dimensional gas chromatography-high resolution mass spectrometry with complementary ionization methods in the study of 5000-year-old mummy.

RATIONALE: Mummification is one of the defining customs of ancient Egypt. The nuances of the embalming procedure and the composition of the embalming mixtures have attracted the attention of scientists and laypeople for a long time. Modern analytical tools make mummy studies more efficient. METHODS: Comprehensive two-dimensional gas chromatography-high resolution mass spectrometry (GCxGC/HRMS) with complementary ionization methods (electron ionization, positive chemical ionization, and electron capture negative ionization [ECNI]) with a Pegasus GC-HRT+4D instrument was used to identify embalming components in the mummy from the Pushkin Museum of Fine Arts acquired in 1913 in London at the de Rustafjaell sale. The mummy dates back to the late Predynastic period (direct accelerator mass spectrometry-dating 3356-3098 bc), being one of the oldest in the world. RESULTS: The results showed the complexity of the embalming mixtures that were already in use 5000 years ago. Several hundred organic compounds were identified in the mummy samples. Various types of hydrocarbons (triterpanes, steranes, isoprenoid, and polycyclic aromatic hydrocarbons) prove the presence of petroleum products. Iodinated compounds detected using ECNI define oils of marine origin, whereas esters of palmitic acid indicate the use of beeswax. The nature of the discovered components of conifer tar proves that the preliminary processing of conifer resins involved heating. GCxGC/HRMS also allowed a number of modern contaminants (phthalates, organophosphates, and even DDT) to be identified. CONCLUSIONS: Application of a powerful GCxGC/HRMS technique with complementary ionization methods allowed significant widening of the range of organic compounds used for mummification that could be identified. The complexity of the embalming mixtures supports the hypothesis of the high social status of the child made on the basis of the preliminary study of the mummy.

Assessment of Thiel-Embalmed Cadavers as a Teaching Tool for Oral Anatomy and Local Anesthesia.

The aim of this study was to determine whether Thiel-embalmed cadavers would provide a useful anatomy teaching tool for topics that cannot be approached using formalin-fixed cadavers such as oral cavity examination and maxillary anesthesia. The suitability of Thiel-embalmed bodies for performing oral examinations was assessed by asking first-year dental and dental hygiene students at a dental school in Ireland to identify oral structures on a classmate and on a Thiel-embalmed body. The study was conducted in 2016. The ease of location was compared in the two settings, and their quality was assessed on the cadavers. The suitability of Thiel-embalmed cadavers to teach maxillary anesthesia was assessed by students' performing mock injections at five adjacent sites daily for five consecutive days, followed by inspection of the gingival surface by experienced anatomists and dentists. Data were obtained from 57 students, but only the 54 forms that were fully completed were analyzed, for an overall response rate of 85.7%. The results showed that most oral structures were more difficult to locate on cadavers. The texture and appearance of features in the cadavers were rated at a midpoint between realistic and unrealistic. The relative inexperience of the participants, the accumulation of fixative in the oral cavity, and discoloration were mentioned as potential confounding factors. Visual analysis of images obtained following repeated injections revealed no deterioration of the tissue. Importantly, the puncture marks appeared to reduce over time, suggesting that the gingival tissue maintains some elasticity following Thiel fixation. These findings suggest that Thiel-embalmed cadavers may be a useful tool to provide students more time to localize and study aspects of the oral cavity. Likewise, the recoiling capacity of gingival tissue suggests that Thiel-embalmed cadavers may provide an ideal tool for teaching injection technique of local anesthetics.

The heart of Blessed Anne-Madeleine Remuzat: a biomedical approach of "miraculous" heart conservation.

We present here the results of our inter-disciplinary examination of the mummified heart of Blessed Anne-Madeleine Remuzat (1696-1730). This organ has been examined in the context of a canonization process. This analysis is related to important aspects of the early history of anatomy in Europe, that of "Holy autopsies", and to the relationship between anatomical investigations, Catholic theology, and religious/medical customs. According to anatomical, genetic, toxicological, and palynological analyses, it has been shown that this organ has not been naturally ("miraculously") conserved but embalmed using myrtle, honey, and lime. Moreover, a right ventricle dilatation has been diagnosed, that may represent a post-tuberculosis condition and may have played a role in the cause of death of this religious figure.

Evidence for prehistoric origins of Egyptian mummification in late Neolithic burials.

Traditional theories on ancient Egyptian mummification postulate that in the prehistoric period (i.e. the Neolithic and Chalcolithic periods, 5th and 4th millennia B.C.) bodies were naturally desiccated through the action of the hot, dry desert sand. Although molding of the body with resin-impregnated linen is believed to be an early Pharaonic forerunner to more complex processes, scientific evidence for the early use of resins in artificial mummification has until now been limited to isolated occurrences during the late Old Kingdom (c. 2200 B.C.), their use becoming more apparent during the Middle Kingdom (c. 2000-1600 BC). We examined linen wrappings from bodies in securely provenanced tombs (pit graves) in the earliest recorded ancient Egyptian cemeteries at Mostagedda in the Badari region (Upper Egypt). Our investigations of these prehistoric funerary wrappings using a combination of gas chromatography-mass spectrometry (GC-MS) and thermal desorption/pyrolysis (TD/Py)-GC-MS have identified a pine resin, an aromatic plant extract, a plant gum/sugar, a natural petroleum source, and a plant oil/animal fat in directly AMS-dated funerary wrappings. Predating the earliest scientific evidence by more than a millennium, these embalming agents constitute complex, processed recipes of the same natural products, in similar proportions, as those utilized at the zenith of Pharaonic mummification some 3,000 years later. The antibacterial properties of some of these ingredients and the localized soft-tissue preservation that they would have afforded lead us to conclude that these represent the very beginnings of experimentation that would evolve into the famous mummification practice of the Pharaonic period.

Radiological evaluation of the evisceration tradition in ancient Egyptian mummies.

Descriptions of the preparation of ancient Egyptian mummies that appear in both scientific and popular literature are derived largely from accounts by the Greek historians Herodotus and Diodorus Siculus. Our reliance on these normative descriptions obscures the wide range of techniques practised, and so stifles the study of geographic, chronological, and social variations in the practice. Using published descriptions in the literature for 150 mummies and 3D reconstructions from computed tomography data for 7 mummies, this study compares empirical data with classical descriptions of evisceration, organ treatment, and body cavity treatment. Techniques for accessing the body cavity, removal and treatment of the organs, and treatment of the eviscerated body cavity vary with time period, sex, and status, and are discussed in relation to their treatment in the literature and their radiological appearance. The Herodotean and Diodorean stereotypes, including the restriction of transabdominal evisceration to the elite and cedar oil enema evisceration to commoners, are falsified by the data. The transperineal forms are present only in elites, and chemical evisceration is not apparent at all. Additionally, the dogmatic contention that the heart was universally retained in situ, or replaced if accidentally removed, is also greatly exaggerated.

Laser Doppler vibrometric assessment of middle ear motion in Thiel-embalmed heads.

HYPOTHESIS: Thiel-embalmed human whole head specimens represent an alternative model in middle ear research. BACKGROUND: Research into middle ear mechanics and the evaluation of active middle ear implants are generally performed on fresh human temporal bone specimens. A drawback of this method is the limited period during which the specimen can be used before tissue decay begins. Tissue conservation that preserves mechanical properties is desirable. METHODS: Using laser Doppler vibrometry, the movement of the tympanic membrane, stapes, and round window were measured in 23 ears from 15 human whole head specimens embalmed according to Thiel. The ears were acoustically stimulated through the intact external auditory canal. The measurement results were compared with data from the literature. RESULTS: The results were similar to those measured in living subjects and from fresh temporal bones, after excluding measurements with nontypical shapes or low amplitudes, that is, 9% for tympanic membrane, 38% for stapes, and 67% for round window. The best agreement was found for the tympanic membrane (mean difference between 0 and 5.2 dB) and for the stapes measurements (mean difference between 0 and 7.4 dB). Larger differences were found for the round window measurements (mean difference between 3 and 19 dB). For the stimulation levels used, the Thiel specimens behaved linearly in amplitude. The results remained reproducible for more than 20 hours in ambient air, and when the specimen was put back into Thiel solution, the measurements were stable for up to several months between measurements. CONCLUSION: Our results showed that the middle ears of Thiel-embalmed human whole head specimens can be used to study human middle ear mechanics; however, significant differences in some frequencies, particularly at the round window, have to be considered.

The microscopic (optical and SEM) examination of putrefaction fluid deposits (PFD). Potential interest in forensic anthropology.

This article describes the potential interest in physical and forensic anthropology of the microscopic analysis of residues of putrefaction fluid, a calcified deposit frequently found associated with bone rests. Its sampling and analysis seem straightforward and relatively reproducible. Samples came from archeological material (Monterenzio Vecchia, an Etruscan necropolis from the north of Italy dated between the fifth and third century B.C.; body rests of Agnès Sorel, royal mistress died in 1450 A.D.; skull and grave of French King Louis the XI and Charlotte of Savoy dated from 1483 A.D.). All samples were studied by direct optical microscope and scanning electron microscopy. Many cytological, histological, and elemental analysis were possible, producing precious data for the identification of these remains and, in some cases, the cause of death.

Artificial mummies from the Andes.

In 1997 agricultural workers, turned into looters, found an intact funerary site in the cloud forest in northeastern Peru. A prompt archaeological rescue project permitted the recovery of an important collection of mummies and artifacts that are providing important insights about the archaeology of the Chachapoya people that established in this area around 900 AD up to the Inca conquest of this territory around the year 1475. The mummies recovered showed evidence of cultural practices devised and used to assure the preservation of the human bodies. Such practices are also reported for among Chinchorro and Chiribaya mummies in the Andes. A cultural interpretation of these funerary activities is discussed connecting the practice of the cult to the ancestors to the access and management of resources and territory.

Characterisation of embalming materials of a mummy of the Ptolemaic era. Comparison with balms from mummies of different eras.

Gas chromatography-mass spectrometry has been used to determine the nature of organic materials used in mummification balms. A comparative analysis of samples taken from Egyptian mummies is developed. The results are given in two parts. First, it is shown that the chemical composition of the balm is practically independent of the part of the mummy from which it is taken. This study was done on a Ptolemaic mummy (circa 100 BC from the Guimet Museum in Lyon). Fats, beeswax, and diterpenic resins were the main components: they were found everywhere. Castor oil was also very often detected (in half of the samples). This particular fat is present in the balm inside the thorax but not in the skull. Moreover it is shown that a vegetable tannin was employed. Components indicative of vegetable tannin input (gallic acid and inositols) were found in seven samples out of eighteen, particularly close to the body and on the canopic pack of the heart. Secondly, some conclusions from a comparative study of the composition of balms from mummies of various social levels as well as of different Egyptian periods are reported. It is shown that beeswax was used as from very early times (XVIIIth dynasty). The mixture of beeswax, fats, and diterpenoid resins would appear to be more recent. The balms of three mummies dating from more recent Egyptian periods (XIXth to XXVth dynasty) were analysed. No evidence of a resin, gum-resin, or plant gum could be found. Some mummies would appear to have been embalmed with fats or beeswax. Finally, the entrails canopic pack said to belong to Ramses II undoubtedly shows an embalming process with a triterpenic resin of the mastic type. The adopted analytical methodology enabled us to achieve simultaneous detection of four components of the balm of the Ptolemaic mummy. Analysis of the other five mummies revealed far less complex chemical compositions for the balms. This may be an indication of different embalming processes, although we should bear in mind the question of organic matter preservation through the ages.

[A sketch of history of the European tanatopraxis]. / Zarys dziejów tanatopraksji europejskiej.

Tanatopraxis is an intentional action aiming to stop all the processes, which lead to a complete decomposition of a body after death. The words embalmment or mummification makes us think about cultures of the ancient Egypt or pre-Columbian America and not about burials of Indo-European nomads or Chinese aristocrats. Meanwhile practices aiming to protect mortal remains against a destructive force of death were known in Europe very long ago. We can see it in paludal burials of the Iron Age and in some testimonies of the ancient epochs. Interventions, which could materially retard decomposition of a body, had been rather generally practiced from the Middle Ages. More practical than religious reasons justified it, but the latter cannot be overlooked. The religious background of such actions can be seen in the custom of embalming mortal remains of popes or kings of France. It was in Europe where the preservation of crops had started to be treated as a scientific problem, and we can see it for the first time in the Renaissance. Together with a development of medical knowledge and especially of anatomy and preparations, a great progress both in methods and substances used for embalming, took place. Ruysh and Holmes in the 17th century, the Hunter brothers in the 18th century and Gannal and Holmes in the 19th century are the milestones in the history of tanatopraxis in Europe. Foundation of the French Institute of Tanatopraxis (IFT) has crowned the many hundred years old experience in the field.

Neurociencia en el Egipto faraónico y en la escuela de Alejandría / Neuroscience in ancient Egypt and in the school of Alexandria

Introducción. A orillas del Nilo floreció hace cinco milenios una de las civilizaciones más antiguas, importantes y duraderas de la historia de la humanidad. En Egipto se alcanzó un alto grado de desarrollo en todas las ramas del saber humano y hoy se considera la civilización madre de la medicina. A pesar del marcado componente religioso que afectaba a todas sus actividades, la medicina faraónica se practicó de una forma racional y deductiva, y los egipcios fueron los creadores de la observación clínica. Desarrollo. El clima desértico del valle del Nilo ha preservado monumentos, momias y papiros cuyo estudio permite hacerse cierta idea del grado de desarrollo alcanzado en los saberes médicos. Su análisis desde el punto de vista de los conocimientos neurocientíficos constituye el objetivo de este trabajo. Los hallazgos anatómicos de los egipcios derivaron de la inspección de heridas y de la práctica del embalsamamiento. Llegaron a conocer un gran número de diagnósticos y a prescribir muchos tratamientos, y alcanzaron cierto desarrollo en el manejo del paciente neurotraumatológico. Practicaron la anamnesis, el pronóstico y una cirugía reglada que incluía infrecuentemente la trepanación. Su conservadurismo hizo que, tras la dominación macedónica, la medicina griega suplantara a la tradicional egipcia, que alcanzó su máximo esplendor con la escuela de Alejandría, donde destacaron Herófilo y Erasístrato, pioneros de los estudios de anatomía y circulación cerebrales. Conclusiones. Desde el punto de vista de la neurociencia, los egipcios describieron por primera vez el cerebro, la jaqueca, la epilepsía, los ictus, el tétanos, la parálisis de Bell y las secuelas de los traumatismos craneales y de la sección medular. En sus manifestaciones artísticas se aprecian pacientes neurológicos y, según refiere Heródoto, había medicos especializados en las `enfermedades de la cabeza' que podrían considerarse precursores de los actuales neurólogos (AU)

Mummification to plastination. Revisited.

A brief summary of the history of anatomy is presented with particular focus on important scientists who contributed significantly to the development of anatomical education and recognition of anatomy as a discipline of science. An attempt has been made to review the history of preservation of human cadavers from earliest mummification methodology used by ancient Egyptians to the most modern technique of embalming and plastination of human cadavers, developed by Von Hagens during the period from 1979 to 1987. The need for use of modern teaching aids for instruction of topographic anatomy is also highlighted.

Movements of vertebrae during manipulative thrusts to unembalmed human cadavers.

OBJECTIVE: The purpose of this study was to measure the relative movements of vertebrae during manipulative thrusts to unembalmed post-rigor mortis human cadavers. SETTING: The investigation was conducted in the gross anatomy laboratory at the University of Calgary. SUBJECTS: Two 77-yr-old, unembalmed, post-rigor mortis, male cadavers were used. INTERVENTIONS: The movements of vertebrae were investigated by using high-speed cinematography to record the movements of bone pins threaded into T10, T11 and T12 during spinal manipulative therapy to unembalmed post-rigor human cadavers. A single clinician delivered a series of posterior-to-anterior (p-to-a) thrusts to the right transverse process of either T10, T11 or T12, using a reinforced hypothenar contact. Relative p-to-a and lateral translations, as well as axial and sagittal rotations, in T10-T11 and T11-T12 were calculated. Corresponding p-to-a forces exerted by the clinician onto the cadaver were recorded using a pressure pad. MAIN RESULTS: Significant relative movements were measured primarily between the targeted and immediately adjacent vertebrae during the thrusts. Vertebral pairs remained slightly 'hyper-extended' after the rapid thrusts to T11, when the p-to-a forces returned to preload levels. CONCLUSIONS: These findings may be useful for the understanding of the deformation behavior of the vertebral column during therapeutic manipulation. A fully three-dimensional analysis of all six degrees of freedom, using a larger number of unembalmed cadavers, would be useful in clarifying the relationship between the externally applied forces and the observed relative movement patterns of the vertebrae.