RESUMO
This study investigates the protective effect of Rosa damascena essential oil on diabetes-induced testicular damage in rats. Thirty-six male Wistar rats were randomly divided into 6 equal groups: Group I: negative control (no treatment); Group II: positive control (diabetic by alloxan injection); Groups III-VI that rendered diabetic and received, respectively, 50, 100, 200, and 400 µg/kg/day rose oil, orally for 28 days. Rose oil did not significantly change body weight and blood glucose level as compared to positive control. Serum testosterone level of rose oil-treated rats remained statistically the same with both negative and positive control groups (Groups I and II). Rats treated with rose oil especially at 2 higher dosages (Groups V and VI) had higher sperm count and increased diameters of seminiferous tubules as compared to Group II. Rose oil even at the lowest dosage significantly increased cell count of spermatogonia, primary spermatocytes, Sertoli cells, and Leydig cells, with better outcomes for higher dosages. It appears that short-term repeated dose administration of rose oil can dose-dependently improve structural deteriorations of testes and epididymal sperm count in diabetic rats.
Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Diabetes Mellitus Experimental/complicações , Suplementos Nutricionais , Óleos Voláteis/uso terapêutico , Orquite/prevenção & controle , Estresse Oxidativo , Rosa/química , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/efeitos adversos , Antioxidantes/administração & dosagem , Antioxidantes/efeitos adversos , Antioxidantes/uso terapêutico , Glicemia/análise , Diabetes Mellitus Experimental/sangue , Suplementos Nutricionais/efeitos adversos , Epididimo/imunologia , Epididimo/metabolismo , Epididimo/patologia , Epididimite/complicações , Epididimite/metabolismo , Epididimite/patologia , Epididimite/prevenção & controle , Masculino , Óleos Voláteis/administração & dosagem , Óleos Voláteis/efeitos adversos , Orquite/complicações , Orquite/metabolismo , Orquite/patologia , Distribuição Aleatória , Ratos Wistar , Túbulos Seminíferos/imunologia , Túbulos Seminíferos/metabolismo , Túbulos Seminíferos/patologia , Contagem de Espermatozoides , Espermatogênese , Testículo/imunologia , Testículo/metabolismo , Testículo/patologia , Testosterona/sangue , Testosterona/metabolismoRESUMO
Rams which were vaccinated at 6-8 months of age with a water-in-oil Brucella ovis-vitamin E adjuvant vaccine had significantly higher serum antibody levels than rams vaccinated with a commercial B. ovis bacterin or B. melitensis Rev 1. The adjuvant vaccine did not cause abscesses at the site of injection as some water-in-oil emulsions do. Two years after vaccination, the vitamin E adjuvant-vaccinated rams had higher antibody level than the other groups. This was most likely due to a secondary response to naturally occurring infection with B. ovis.
Assuntos
Adjuvantes Imunológicos/farmacologia , Vacina contra Brucelose/imunologia , Ovinos/imunologia , Vitamina E/farmacologia , Animais , Anticorpos Antibacterianos/biossíntese , Brucella/imunologia , Brucelose/prevenção & controle , Brucelose/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Epididimite/prevenção & controle , Epididimite/veterinária , Masculino , Doenças dos Ovinos/prevenção & controleRESUMO
Rams vaccinated at 7 and 8 months of age with a B. ovis-vitamin E adjuvant vaccine had increased antibody titers compared with Freund's incomplete adjuvant or commercial bacterin vaccinated rams. The percent overall infectivity in an experimental infection of B. ovis-vitamin E adjuvant vaccinated rams was 22% compared to 44% for B. ovis-Freund's incomplete adjuvant or bacterin vaccinated rams and 67% for control.