Life cycle assessment for evaluation of novel solvents and technologies: A case study of flavonoids extraction from Ginkgo biloba leaves.

Innovative solvents such as deep eutectic solvents (DESs) and process intensification technologies assisted by ultrasound have been demonstrated to be promising pathways for enhancing solid-liquid extraction. Nevertheless, quantitative and systematic knowledge of their environmental impact is still limited. In this work, a case study of flavonoids extraction from Ginkgo biloba leaves was evaluated by using life cycle assessment (LCA) for comparison of three extraction scenarios. The first used DES as extractant (DESE), and the other two adopted ethanol, including heat reflux extraction (HRE), and ultrasound-assisted extraction (UAE). Among eight key midpoints investigated, all these from UAE were 10.0 %-80.0 % lower than from DESE and HRE except water consumption. The UAE was the eco-friendliest option due to its higher extraction yield, shorter duration and lower solvent consumption. The DESE exhibited the lowest water consumption, the highest freshwater ecotoxicity and human carcinogenic toxicity, while HRE had the highest impacts for the other 6 midpoints. Moreover, solvent production was the key contributor for all the categories. The standardized sensitivity analysis showed that the overall environmental footprint can be further decreased by 15.4 % for DESE pathways via substituting choline chloride/glycerine with choline chloride/ethylene glycol. Furthermore, all pathways using DESs had higher standardized impacts than those employing ethanol from sugarcane or wood. Replacing ethanol from maize with other feedstocks can significantly lessen the overall impacts, among which the UAE using ethanol from sugarcane demonstrated the least environmental impacts. The promotion of DESs as "green and sustainable" alternative to traditional solvents requires careful consideration.

Sustainable valorization of macroalgae residual biomass, optimization of pyrolysis parameters and life cycle assessment.

The major challenges for the current climate change issue are an increase in global energy demand, a limited supply of fossil fuels, and increasing carbon footprints from fossil fuels, which have necessitated the exploration of sustainable alternatives to fossil fuels. Biorefineries offer a promising path to sustainable fuel production, converting biomass into biofuels using diverse technologies. Aquatic biomass, such as macroalgae in this context, represents an abundant and renewable biomass resource that can be cultivated from water bodies without competing with traditional agricultural land. Despite this, the potential of macroalgae for biofuel production remains largely untapped, with very limited studies addressing their viability and efficiency. This study investigates the efficient conversion of unexplored macroalgae biomass through a biorefinery process that involves lipid extraction to produce biodiesel, along with the production of biochar and bio-oil from the pyrolysis of residual biomass. To improve the effectiveness and overall performance of the pyrolysis system, Response Surface Methodology (RSM) was utilized through a Box-Behnken design to systematically investigate how alterations in temperature, reaction time, and catalyst concentration influence the production of bio-oil and biochar to maximize their yields. The results showed the highest bio-oil yield achieved to be 36 %, while the highest biochar yield reached 45 %. The integration of Life Cycle Assessment (LCA) in the study helps to assess carbon emission and environmental burdens and identify potential areas for optimization, such as resource efficiency, waste management, and energy utilization. The LCA results contribute to the identification of potential environmental hotspots and guide the development of strategies to optimize the overall sustainability of the biofuel production process. The LCA results indicate that the solvent (chloroform) used in transesterification contributes significantly to greenhouse gas emissions and climate change impacts. Therefore, it is crucial to explore alternative, safe solvents that can mitigate the environmental impacts of transesterification.

Life cycle assessment perspective on waste resource utilization and sustainable development: A case of glyphosate production.

The growing demand for pesticide manufacturing and increasing public awareness of sustainable development, have let to urgent requirements for a refined environmental management framework. It is imperative to conduct process-based life cycle assessments (LCAs) to promote clean and environment-friendly technologies. Herein, the cradle-to-gate LCA of glyphosate production was executed as an example to investigate crucial production factors (materials or energy) and multiple environmental impacts during the production processes. Results showed that methanol caused the highest environmental damage in terms of toxicity, with a normalized value of 85.7 × 10-8, followed by coal-fired electricity in 6.00 × 10-8. Furthermore, optimized schemes were proposed, including energy improvement (electricity generated by switching from coal-fired power to solar power) and wastewater targeted conversion. Regarding the normalization results before and after optimization, the latter showed more significant results with the normalized value decreasing by 21.10 × 10-8, while that of the former only decreased by 6.50 × 10-8. This study provides an integrated LCA framework for organophosphorus pesticides (OPs) from upstream control and offers an important supplement to managing the key pollution factors and control links of the OP industry. Moreover, it reveals the positive influence of optimized schemes in facilitating cleaner production technologies, thus ultimately promoting new methodologies for resource recycling.

Biochar-integrated reactive filtration of wastewater for P removal and recovery, micropollutant catalytic oxidation, and negative CO2 e: Life cycle assessment and techno-economic analysis.

Life cycle assessment (LCA) and techno-economic analysis (TEA) models are developed for a tertiary wastewater treatment system that employs a biochar-integrated reactive filtration (RF) approach. This innovative system incorporates the utilization of biochar (BC) either in conjunction with or independently of iron-ozone catalytic oxidation (CatOx)-resulting in two configurations: Fe-CatOx-BC-RF and BC-RF. The technology demonstrates 90%-99% total phosphorus removals, adsorption of phosphorus to biochar for recovery, and >90% destructive removal of observed micropollutants. In this work, we conduct an ISO-compliant LCA of a 49.2 m3 /day (9 gpm) field pilot-scale Fe-CatOx-BC-RF system and a 1130 m3 /day (0.3 MGD) water resource recovery facility (WRRF)-installed RF system, modeled with BC addition at the same rate of 0.45 g/L to quantify their environmental impacts. LCA results indicated that the Fe-CatOx-BC-RF pilot system is a BC dose-dependent carbon-negative technology at -1.21 kg CO2 e/m3 , where biochar addition constitutes a -1.53 kg/m3 CO2 e beneficial impact to the process. For the WRRF-installed RF system, modeled with the same rate of BC addition, the overall process changed from 0.02 kg CO2 e/m3 to a carbon negative -1.41 kg CO2 e/m3 , demonstrating potential as a biochar dose-dependent negative emissions technology. Using the C100 100-year carbon accounting approach rather than Cnet reduces these CO2 e metrics for the process by about 25%. A stochastic TEA for the cost of water treatment using this combinatorial P removal/recovery, micropollutant destructive removal, and disinfection advanced technology shows that at scale, the mean cost for treating 1130 m3 /day (0.3 MGD) WRRF secondary influent water with Fe-CatOx-BC-RF using the C100 metric is US$0.18 ± US$0.01/m3 to achieve overall process carbon neutrality. Using the same BC dose in an estimation of a 3780 m3 /day (1 MGD) Fe-CatOx-BC-RF facility, the carbon neutral cost of treatment is reduced further to US$0.08 ± $0.01 with added BC accounting for US$0.03/m3 . Overall, the results demonstrate the potential of carbon negativity to become a water treatment performance standard as important and attainable as pollutant and pathogen removal. PRACTITIONER POINTS: Life cycle assessment (LCA) of a pilot scale tertiary biochar water treatment process with or without catalytic ozonation at a WRRF shows a carbon negative global warming potential of -1.21-kg CO2e/m3 while removing 90%-99% TP and >90% of detected micropollutants. Biochar-integrated reactive filtration use can aid in long-term carbon sequestration by reducing the carbon footprint of advanced water treatment in a dose-dependent manner, allowing an overall carbon-neutral or carbon-negative process. A companion paper to this work (Yu et al., 2023) presents the details related to the process operation and mechanism and evaluates the pollutant removal performance of this Fe-CatOx-BC-RF process in engineering laboratory pilot research and field WRRF pilot-scale water resource recovery trials. Techno-economic analysis (TEA) of this biochar catalytic oxidation reactive filtration process using Monte Carlo stochastic modeling shows a forecasted carbon-neutral process cost with low P and micropollutant removal as US$0.11/m3 ± 0.01 for a 3780-m3/day (1 MGD) scale installation with BC cost at US$0.03/m3 of that total. The results demonstrate the potential of carbon negativity to become a water treatmentperformance standard as important and attainable as pollutant and pathogen removal.

Crude Oil and Its Burnt Residues Induce Metamorphosis in Marine Invertebrates.

Metamorphosis is a critical process in the life cycle of most marine benthic invertebrates, determining their transition from plankton to benthos. It affects dispersal and settlement and therefore decisively influences the dynamics of marine invertebrate populations. An extended period of metamorphic competence is an adaptive feature of numerous invertebrate species that increases the likelihood of finding a habitat suitable for settlement and survival. We found that crude oil and residues of burnt oil rapidly induce metamorphosis in two different marine invertebrate larvae, a previously unknown sublethal effect of oil pollution. When exposed to environmentally realistic oil concentrations, up to 84% of tested echinoderm larvae responded by undergoing metamorphosis. Similarly, up to 87% of gastropod larvae metamorphosed in response to burnt oil residues. This study demonstrates that crude oil and its burned residues can act as metamorphic inducers in marine planktonic larvae, short-circuiting adaptive metamorphic delay. Future studies on molecular pathways and oil-bacteria-metamorphosis interactions are needed to fully understand the direct or indirect mechanisms of oil-induced metamorphosis in marine invertebrates. With 90% of chronic oiling occurring in coastal areas, this previously undescribed impact of crude oil on planktonic larvae may have global implications for marine invertebrate populations and biodiversity.

Possibilidades de integração entre a fonoaudiologia e a nutrição com base em produções nacionais / Possibilities of integration between speech therapy and nutrition based on national productions / Posibilidades de integración entre la terapia del lenguaje y la nutrición a partir de las producciones nacionales

Introdução: A atuação interdisciplinar entre a fonoaudiologia e a nutrição colabora para um atendimento mais integral entre os usuários da saúde, fato que demonstra a importância de pesquisar suas correlações. Objetivo: O objetivo deste estudo foi verificar se existe integração entre a atuação do fonoaudiólogo e do nutricionista, identificando as publicações existentes, as especialidades contempladas, bem como a atuação dos profissionais nos diferentes ciclos de vida e níveis de atenção à saúde. Metodologia: Foram analisados 3.551 artigos eletrônicos disponíveis eletronicamente dos periódicos CODAS, CEFAC, Distúrbios da Comunicação e Audiology Communication Research, entre os anos de 2013 e 2022, por meio de um Protocolo de Classificação de Artigos. Resultados: Dentre as publicações analisadas, 274 (7,7%) integram a Fonoaudiologia e a Nutrição, especialmente na Especialidade da Motricidade Orofacial (n=124; 26,9%), no Ciclo Vital Infantil (n=132; 43%) e no Nível de Atenção Secundário (n=97; 35,4%). Conclusão: Concluiu-se que existe interface entre as duas profissões, porém, ainda é necessário fortalecer a atuação nos diferentes ciclos de vida. Além disso, incentivar a prática profissional em outros níveis de atenção, especialmente na primária, para prevenção dos distúrbios alimentares e promoção da alimentação saudável entre usuários do sistema de saúde.

Introduction: Interdisciplinary performance between speech therapy and nutrition contributes to a more integral care among health users, a fact that demonstrates the importance of researching their correlations. Objective: The purpose of this study was to verify if there is integration between the performance of the speech therapist and nutritionist, identifying the existing publications, the specialities contemplated, as well the performance of professionals in different life cycles and levels of health care. Methods: 3.551 electronic articles were analyzed, in the electronic journals CODAS, CEFAC, Distúrbios da Comunicação and Audiology Communication Research, between the years 2013 and 2022, through an Article Classification Protocol. Results: Among the analyzed publications, 274 (7,7%) integrate Speech Language and Hearing Sciences and Nutrition, especially in orofacial motricity (n=124; 26,9%), Children's Life Cycle (n=132; 43%), and secondary care level (n=97; 35,4%). Conclusion: it was concluded that there is an interface between the two professions, although it is still necessary to strengthen the performance in the different life cycles. Furthermore, to encourage professional practice at other levels of care, especially in the primary, to prevent nourishment disorders and promote healthy eating among users of the health system

Introducción: El rendimiento interdisciplinario entre la terapia del habla y la nutrición contribuye a una atención más integral entre los usuarios de la salud, un hecho que demuestra la importancia de investigar sus correlaciones. Objetivo: El objetivo de este estudio fue verificar si existe integración entre la terapia del habla y la nutricionista, identificando las publicaciones existentes, las especialidades contempladas, así como la actuación de los profesionales en diferentes ciclos de vida y niveles de atención a la salud. Métodos: Fueron analizados 3.551 artículos electrónicos, en las revistas electrónicas CODAS, CEFAC, Distúrbios da Comunicação y Audiology Communication Research, entre los años 2013 a 2022, a través de un Protocolo de Clasificación de Artículos. Resultados: Entre las publicaciones analizadas, 274 (7,7%) integran terapia del habla y Nutrición, especialmente en motricidad orofacial (n=124; 26,9%), Ciclo de Vida Infantil (n=132; 43%), y secundaria nivel asistencial (n=97; 35,4%). Conclusións: Se concluyó que existe una interfaz entre las dos profesiones, aunque aún es necesario fortalecer el desempeño en los diferentes ciclos de vida. Además, incentivar la práctica profesional en otros niveles de atención, especialmente en el primario, para prevenir trastornos de la alimentación y promover la alimentación saludable entre los usuarios del sistema de salud.

Life-cycle selenium accumulation and its correlations with the rhizobacteria and endophytes in the hyperaccumulating plant Cardamine hupingshanensis.

Cardamine hupingshanensis (C. hupingshanensis) is known for its ability to hyperaccumulate selenium (Se). However, the roles of the rhizobacteria or endophytes in Se hyperaccumulation have not been explored in C. hupingshanensis. Here, in-situ-like pot experiments were conducted to investigate the characteristics of Se accumulation throughout C. hupingshanensis growth stages and its correlations with rhizobacteria and endophytes under varying soil Se levels. Results showed that Se levels in roots, stems and leaves increased from the seedling to bolting stage, but remained relatively stable during the flowering and maturity. Leaves exhibited the highest Se levels (736.48 ± 6.51 mg/kg DW), followed by stems (575.39 ± 27.05 mg/kg DW), and lowest in roots (306.62 ± 65.45 mg/kg DW) under high-Se stress. The Se translocation factors from soils to C. hupingshanensis roots was significantly higher (p < 0.05) in low-Se soils compared to medium- and high-Se soils. Rhizobacterial diversity showed significant positive correlations (p < 0.05) with both total and bioavailable soil Se contents. The levels of soil Se and growth stages of C. hupingshanensis were found to have significant effects (p < 0.03) on the compositions of rhizosphere bacteria and C. hupingshanensis endophytes. Low-abundance bacteria (< 5%), including Gemmatimonadetes, Latescibacteria and Nitrospirae, were identified to potentially increase the bioavailable Se levels in the rhizosphere. The Se accumulation significantly decreased (p < 0.05) in C. hupingshanensis grown in sterilized low- (32.4%), medium- (17%) and high-Se (42%) soils. Endophytes in C. hupingshanensis, such as Firmicutes and Proteobacteria, were likely recruited from the rhizobacteria, as evidenced by the isolated bacterial strains, and played an important role in Se hyperaccumulation, particularly during the flowering stage. This study provides new insights into potential mechanism underlying Se hyperaccumulation in C. hupingshanensis.

A life cycle assessment of coal-fired thermal power plants with post-combustion control techniques: an India scenario.

In recent years, there has been a focus on clean power generation, and it is critical to assess the environmental impact of novel technologies used in pollution control in power generation. The study uses life cycle assessment (LCA) to assess the environmental impacts of coal-fired thermal power plants with different emission control techniques in an Indian scenario. As there are no such studies available in the Indian context, this work might provide a holistic view of the impacts of energy generation. A supercritical coal-fired plant with a capacity of 660 MW is considered in this study. The system boundary included coal extraction, transportation, power plant operation, and transmission losses of electricity with a functional unit of 1 kWh. It was observed that there was an energy penalty due to the power consumed in emission control devices, but the maximum energy penalty was due to the power used in the carbon capture system. The LCA is done from "cradle to gate", with impact indicators at the mid-point evaluated using the RECIPE (H) 2016 LCIA method. LCA results showed that power plant operation is the most significant contributor to environmental impact. Initially, in cases 1 and 2, climate change (CC) potential was a major impact category, but CC potential was reduced with carbon capture and storage, 0.27 kg CO2 eq. in case 3 with ESP, FGD, SCR, and carbon capture and storage (CCS) and 0.263 kg CO2 eq. in case 4 with ESP and CCS. But there was a considerable increase in the majority of the impact categories in case 4. Freshwater consumption potential increased from 3.98 E-03 m3 in base case 1 to 4.98 E-03 m3 in case 3 due to the amount of water used in chemical production during CCS, as CC potential is a major concern in power generation, However, compared to case 1, the potential for climate change increased in case 2, whilst in case 4, the potential for climate change is lower but has resulted in an increase in the majority of impact categories. Case 3 shows an optimal approach to reducing CO2 emissions compared to other cases. The combination of ESP, FGD, SCR, and CCS is favourable for cleaner energy generation.

Agriculture and biodiversity damage: A prospective evaluation of the impact of Brazilian agriculture on its ecoregions through life cycle assessment methodology.

The natural ecosystems' replacement by farmland and the consequent biodiversity damage (BD) for agriculture are one of the principal concerns worldwide. The development of the life cycle assessment (LCA) methodology involves enormous efforts to include BD parameters and develop a prospective LCA approach for future evaluations of production and technologies use. Thus, this work aims to determine the current impacts and estimate the future impacts in terms of damage to biodiversity caused by land occupation by agricultural commodities produced in Brazil, such as coffee, corn, oranges, and sugar cane, for the six ecoregions present in the country-Amazon, Atlantic Forest, Caatinga, Cerrado, Pampas, and Pantanal-in the 20-year period from 2015 to 2035. For this and to search for hotpots, we applied the indicators proposed by Chaudhary and Books (2018), for inventories whose functional unit is production per m2 of 1 kg of crop. Although the Cerrado is one of the ecoregions in which deforestation has advanced the most, it has the area/production ratio that has evolved the most. In contrast, Pampas and Caatinga, which are not seen as agricultural frontiers, increased their impacts. The most optimistic scenarios for the future have been those in regions considered agricultural frontiers; however, these are the regions where agriculture is more technologically developed, for example, coffee production in the Atlantic Forest and in the Cerrado. The results indicate that the technological development of agriculture can contribute to mitigating the impacts of damage to biodiversity in the future, and that the implementation of legislative and inspection measures is fundamental to supporting the correct use of the soil and preventing illegal soil change. Otherwise, in the future, we will see the increasing disappearance of species. Thus, we need researchers, farmers, and policy makers to move from development to conservation.

Benthic ecosystem determines jellyfish blooms by controlling the polyp colony development.

The harmful irregular jellyfish blooms in recent years are difficult to be deciphered by macro hydrographic condition changes. To fundamentally explain the dynamic of jellyfish populations, we shifted the focus to the polyp stage of jellyfish life cycle and local benthic ecosystems. We monitored the population dynamics of Aurelia coerulea polyps in Jiaozhou Bay and other benthic biofouling species in situ to explore the adaptive mechanism of polyps and interspecific interactions in the benthic microhabitat. Our results showed that as temperature increased, the polyps multiplied on the bare substrate, however, other benthic fouling organisms simultaneously invaded the polyp colony according to their different colonisation methods and physiological characteristics. In addition, the polyps were extremely tolerant to food scarcity in the natural environment at low temperatures. Our study indicated that it is necessary to consider the local benthic ecosystem and implement ecosystem-based management strategies to predict and manage problematic jellyfish blooms.

Environmental life cycle assessment of nutraceuticals: A case study on methylcobalamin in different packaging types.

The growing production of pharmaceuticals and nutraceuticals, e.g., methylcobalamin supplements, improves the health of people. This study assesses the environmental footprint of chewable methylcobalamin supplements in four packaging types: blister packs or bottles made of HDPE, PET, or glass. A cradle-to-grave life cycle assessment is conducted to evaluate the supply to Belgian consumers of the recommended daily dose of methylcobalamin supplementation (1.2 mg) in case of deficiency. The impact of methylcobalamin manufacturing in major producing countries (China as baseline and France) is analyzed based on detailed synthesis modeling of data points coming from patents. The overall carbon footprint (CF) is dominated by the transport of consumers to the pharmacy and methylcobalamin powder manufacturing in China (while its mass share per supplement is only 1 %). The impact is the lowest for supplements in HDPE bottles (6.3 g CO2 eq) and 1 %, 8 %, and 35 % higher for those in PET bottles, glass bottles, and blister packs, respectively. Tablets in blister packs have for other investigated impact categories (fossil resource footprint (FRF); acidification; eutrophication: freshwater, marine, and terrestrial; freshwater ecotoxicity; land use; and water use) the highest footprint and those in HDPE and PET bottles for most the lowest. The CF of methylcobalamin powder manufacturing in France is 22 % lower than in China (2.7 g CO2 eq), while the FRF is similar in both locations (26-27 kJ). The FRF and the difference in the CF are chiefly due to energy use and solvent production emissions. Similar trends as the CF are found for other investigated impact categories. Valuable conclusions are drawn for environmental studies on pharmaceuticals and nutraceuticals: (i) including accurate data on consumer transport, (ii) using more environmentally-friendly active ingredients, (iii) choosing appropriate packaging types considering multiple aspects: convenience, environmental footprint, etc., and (iv) providing a holistic picture through assessing various impact categories.

The Life Cycle of Aurelia aurita Depends on the Presence of a Microbiome in Polyps Prior to Onset of Strobilation.

Aurelia aurita's intricate life cycle alternates between benthic polyp and pelagic medusa stages. The strobilation process, a critical asexual reproduction mechanism in this jellyfish, is severely compromised in the absence of the natural polyp microbiome, with limited production and release of ephyrae. Yet, the recolonization of sterile polyps with a native polyp microbiome can correct this defect. Here, we investigated the precise timing necessary for recolonization as well as the host-associated molecular processes involved. We deciphered that a natural microbiota had to be present in polyps prior to the onset of strobilation to ensure normal asexual reproduction and a successful polyp-to-medusa transition. Providing the native microbiota to sterile polyps after the onset of strobilation failed to restore the normal strobilation process. The absence of a microbiome was associated with decreased transcription of developmental and strobilation genes as monitored by reverse transcription-quantitative PCR. Transcription of these genes was exclusively observed for native polyps and sterile polyps that were recolonized before the initiation of strobilation. We further propose that direct cell contact between the host and its associated bacteria is required for the normal production of offspring. Overall, our findings indicate that the presence of a native microbiome at the polyp stage prior to the onset of strobilation is essential to ensure a normal polyp-to-medusa transition. IMPORTANCE All multicellular organisms are associated with microorganisms that play fundamental roles in the health and fitness of the host. Notably, the native microbiome of the Cnidarian Aurelia aurita is crucial for the asexual reproduction by strobilation. Sterile polyps display malformed strobilae and a halt of ephyrae release, which is restored by recolonizing sterile polyps with a native microbiota. Despite that, little is known about the microbial impact on the strobilation process's timing and molecular consequences. The present study shows that A. aurita's life cycle depends on the presence of the native microbiome at the polyp stage prior to the onset of strobilation to ensure the polyp-to-medusa transition. Moreover, sterile individuals correlate with reduced transcription levels of developmental and strobilation genes, evidencing the microbiome's impact on strobilation on the molecular level. Transcription of strobilation genes was exclusively detected in native polyps and those recolonized before initiating strobilation, suggesting microbiota-dependent gene regulation.

Carbon accounting of negative emissions technologies integrated in the life cycle of spirulina supplements.

Carbon dioxide removal (CDR) technologies are considered essential to accomplish the Paris Agreement targets. Given the important contribution of the food sector to climate change, this study aims to investigate the role of two carbon capture and utilization (CCU) technologies in decarbonizing the production of spirulina, an algae product commonly consumed for its nutritional characteristics. The proposed scenarios considered the replacement of synthetic food-grade CO2 in Arthrospira platensis cultivation (BAU scenario) with CO2 from beer fermentation (BRW) and CO2 from DACC (direct air carbon capture) (SDACC), representing two alternatives with great potential in the short and medium-long term, respectively. The methodology follows the Life Cycle Assessment guidelines, considering a cradle-to-gate scope and a functional unit equivalent to the annual production of spirulina in a Spanish artisanal plant. Results showed a better environmental performance of both CCU scenarios as compared to BAU, reaching a reduction of greenhouse gas (GHG) emissions of 52 % in BRW and of 46 % in SDACC. Although the brewery CCU offers a deeper carbon mitigation of spirulina production, the process cannot reach net zero GHG emissions due to residual burdens across the supply chain. In comparison, the DACC unit could potentially supply both the CO2 needed in spirulina production and work as a CDR to compensate residual emissions, which opens the door for further research on its technical and economic feasibility in the food sector.

Review: The challenge to integrate animal welfare indicators into the Life Cycle Assessment.

The transition to a more sustainable livestock sector represents one of the major challenges of our time. Life Cycle Assessment (LCA) is recognised as the gold standard methodology for assessing the environmental impact of farming systems. Simultaneously, animal welfare is a key component of livestock production and is intrinsically related to human and environmental well-being. To perform an overall on-farm sustainability assessment, it would be desirable to consider both the environmental impact and the welfare of the animals. The present work aimed to summarise and describe the methodologies adopted in peer-reviewed papers published to date, that combine animal welfare evaluation with LCA. Citations, retrieved from four bibliographical databases, were systematically evaluated in a multi-stage approach following the JBI and PRISMA scoping review guidelines. The searches identified 1 460 studies, of which only 24 were compliant with the inclusion criteria. The results highlighted how the environmental LCA was undertaken with a much more homogenous and standardised method than animal welfare assessment. When studies were grouped based on the type of animal welfare assessment performed: 16.7% used single welfare indicators, 45.8% multiple indicators, 8.3% applied existing validated protocols (i.e., TGI-200 and TGI-35L), 16.7% used non-validated protocols and 12.5% employed other methods. The papers were further classified with respect to the "5 Animal Welfare Domains Model": the most assessed domain was "environment" (90.5% of the papers%), followed by "health" (52.4%), "nutrition" (33.3%), "behavioural interactions" (28.6%) and "mental state" (9.5%). None of the studies assessed all the domains simultaneously. In addition, 66.7% of papers (n = 16) aggregated the animal welfare indicators into a final score. Within these, only four papers proposed to associate the animal welfare scores with the LCA functional unit. An overall sustainability score, calculated with several different approaches to summarise the information, was provided by 46% of the papers. In summary, despite the topic's relevance, to date, there is neither a consensus on the animal welfare assessment approach to be carried out (indicators selection and their aggregation) nor on the standardisation of an integrated animal welfare-LCA evaluation. The present review provides a basis for the development of common future guidelines to carry out a comprehensive, true-to-life and robust farm sustainability assessment.

Identifying the environmental hotspots of sulfur-free odorant for LPG storage and filling by life cycle approach.

The main objective of this study is to determine the potential environmental impact of storage and filling the liquefied petroleum gas (LPG) with sulfur-containing (ethyl mercaptan) and sulfur-free (Greenodor) odorants by comparative life cycle assessment (LCA). The LCA was carried out within the scope of ISO 14040 and 14044 Standards in a facility that stores and fills LPG and potential environmental impact was calculated for eleven different impact categories. According to the characterization results, it was determined that the overseas transportation process had the highest impact among all impact categories. Because environmental impact was suppressed by other processes in characterization results due to the very low inclusion of the odorants in LPG, the percentage contribution of consumption of both odorants was compared and it was revealed that Greenodor had a lower environmental impact in all mid-point impact categories. For both tanker and cylinder filling, the impact category with the highest difference was photochemical oxidation with a rate of 79 %. The lowest difference was found in the global warming impact category with 18 % for tanker filling and 19 % for cylinder filling. Considering uncertainty analysis results for LPG tanker filling, Greenodor preceded ethyl mercaptan for all mid-point categories. However, in terms of LPG cylinder filling, there was no significant difference between two odorants.

Application of life cycle assessment and machine learning for the production and environmental sustainability assessment of hydrothermal bio-oil.

The hydrothermal bio-oil (HBO) production from biomass conversion can achieve sustainable and low-carbon development. It is always time-consuming and labor-intensive to quantitative relationship between influential variables and bio-oil yield and environmental sustainability impact in the hydrothermal conditions. Machine learning was used to predict bio-oil yield. Life cycle assessment (LCA) is further conducted to assess its environmental sustainability effect. The results demonstrated that gradient boosting decision tree regression (GBDT) have the most optimal prediction performance for the HBO yield (Training R2 = 0.97, Testing R2 = 0.92, RMSE = 0.05, MAE = 0.03). Lipid content is the most significant influential factor for HBO yield. LCA result further suggested that 1 kg of bio-oil production can cause 0.02 kg ep of SO2, 2.05 kg ep of CO2, and 0.01 kg ep of NOx emission, and environmental sustainability assessment of HBO is exhibited. This study provides meaningful insights to ML model prediction performance improvement and carbon footprint of HBO.

Data-driven decarbonisation pathways for reducing life cycle GHG emissions from food waste in the hospitality and food service sectors.

The Hospitality and Food Service (HaFS) sectors are notoriously known for their contribution to the food waste problem. Hence, there is an urgent need to devise strategies to reduce food waste in the HaFS sectors and to decarbonise their operation to help fight hunger, achieve food security, improve nutrition and mitigate climate change. This study proposes three streams to decarbonise the staff cafeteria operation in an integrated resort in Macau. These include upstream optimisation to reduce unserved food waste, midstream education to raise awareness amongst staff about the impact of food choices on the climate and health, and finally downstream recognition to reduce edible plate waste using a state-of-the-art computer vision system. Technology can be an effective medium to facilitate desired behavioural change through nudging, much like how speed cameras can cause people to slow down and help save lives. The holistic and data-driven approach taken revealed great potential for organisations or institutions that offer catering services to reduce their food waste and associated carbon footprint whilst educating individuals about the intricate link between food, climate and well-being.

Integrating life cycle assessment with quantitative microbial risk assessment for a holistic evaluation of sewage treatment plant.

An integrated approach was employed in the present study to combine life cycle assessment (LCA) with quantitative microbial risk assessment (QMRA) to assess an existing sewage treatment plant (STP) at Roorkee, India. The midpoint LCA modeling revealed that high electricity consumption (≈ 576 kWh.day-1) contributed to the maximum environmental burdens. The LCA endpoint result of 0.01 disability-adjusted life years per person per year (DALYs pppy) was obtained in terms of the impacts on human health. Further, a QMRA model was developed based on representative sewage pathogens, including E. coli O157:H7, Giardia sp., adenovirus, norovirus, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The public health risk associated with intake of pathogen-laden aerosols during treated water reuse in sprinkler irrigation was determined. A cumulative health risk of 0.07 DALYs pppy was obtained, where QMRA risks contributed 86 % of the total health impacts. The annual probability of illness per person was highest for adenovirus and norovirus, followed by SARS-CoV-2, E. coli O157:H7 and Giardia sp. Overall, the study provides a methodological framework for an integrated LCA-QMRA assessment which can be applied across any treatment process to identify the hotspots contributing maximum environmental burdens and microbial health risks. Furthermore, the integrated LCA-QMRA approach could support stakeholders in the water industry to select the most suitable wastewater treatment system and establish regulations regarding the safe reuse of treated water.

Multiple reproduction forms in the polyps of the cannonball jellyfish Stomolophus sp. 2: Probable life-cycle reversal.

The jellyfish genera Stomolophus spp. is one of the most abundant in the Pacific Ocean, yet it has not been thoroughly studied. Until recently, research has been developed and directed to its knowledge because of the economic interest in its exploitation. The genus Stomolophus in the Pacific Ocean is composed of five species (S. agaricus, S. chunii, S. collaris, S. fritillaria, and S. meleagris), and Stomolophus sp. 2 has been recently reported in the central part of the Gulf of California. Therefore, this study aimed to describe in vivo the different developmental stages of Stomolophus sp. 2 life cycle. As a result, multiple polyp reproduction forms were described, such as polyp-stolon formation, polydisc strobilation with more than 20 ephyrae formed by each strobila, and polyp formation directly from juvenile ephyra. In the degenerating phase, the polyps turned into cysts induced by stress conditions, such as changes in temperature, oxygen, and food availability. The life cycle of Stomolophus sp. 2 can be distinguished from that of S. meleagris by showing various asexual reproduction mechanisms and polydisc-like strobilation. The formation of polyps directly from the ectoderm of degenerating juvenile medusae suggests the possibility of a reversion cycle. Because of the different life cycles between S. meleagris and S. sp. 2, in addition to their morphological and genetic differences, this study proposes that Stomolophus sp. 2 should be considered a new species and suggests the name Stomolophus yaquilli, in reference to the indigenous community that lives in the species distribution area.

Multi-season environmental life cycle assessment of lemons: A case study in south Uruguay.

Lemons are a relevant agricultural commodity in Uruguay, mainly exported for fresh consumption. Food eco-labels are on the rise worldwide as consumers and authorities are increasingly demanding them. However, there is a lack of scientific studies estimating the environmental impacts of Uruguayan citrus production. This study aims to assess the environmental performance of lemon production in Uruguay taking into account inter-seasonal variability by applying the Life Cycle Assessment (LCA) methodology and following the Environmental Product Declarations (EPDs) guidelines. A cradle-to-farm gate assessment was carried out based on both mass and spatial functional units. Primary data was gathered from a representative orchard of the region for four harvest seasons (2016-2020). Environmental impact categories recommended by EN 15804 + A2 standard were assessed. Specifically, blue water scarcity was assessed using the AWARE method. In addition, human and freshwater ecotoxicity were assessed using USEtox. Results show that on-field emissions and input production are critical for most of the categories assessed (on average, 84% CC, 88% Ac, 98% MEu, and 85% TEu), whereas blue water consumed for irrigation is the main hotspot in blue water scarcity (86%, on average). As expected, inter-seasonal impacts present higher variability when expressing results per tonne vs. per hectare because, although agricultural inputs applied are the same, climatic variability influences water requirements and also affects yield. Blue water scarcity exhibits the highest variability because water consumption depends strongly on agroclimatic conditions, mainly on rain and irrigated water and on water dynamics in soil. Nitrate leaching is a key emission for freshwater eutrophication and, to a minor degree, for climate change, which also depends on the water dose and timing, either from rain or irrigation. Optimising the N application is crucial to minimise on-field emissions, a hotspot in the present study. Along these lines, improved agricultural practices are suggested to enhance the environmental profile of Uruguayan lemons. Replacement or minimisation of the dose of certain inputs (e.g., copper oxide) through the implementation of complementary agricultural practices is suggested. Finally, up-to-date techniques to decrease blue water scarcity are proposed. Methodological recommendations for future studies include modelling N emissions using mechanistic models, incorporating potential reductions in N emissions due to certain agricultural practices, and harmonizing the methodology to quantify water consumption. This study sets a baseline LCA for Uruguayan citrus fruit production. It highlights inter-seasonal variability as an issue to be considered, even when agricultural practices do not change, and especially relevant in countries with high climatic variability like Uruguay. The study also provides scientific and quantitative evidence to support the environmental decisions of both citrus producers and consumers.