RESUMO
BACKGROUND: Traditional Chinese medicine Taraxacum officinale has been widely used to treat various inflammatory diseases. Taraxasterol is one of the main active components isolated from Taraxacum officinale. Recently, we have demonstrated that taraxasterol has the in vitro anti-inflammatory effects. This study aims to determine the in vivo anti-inflammatory effects of taraxasterol against animal models. MATERIALS AND METHODS: Anti-inflammatory effects were assessed in four animal models by using dimethylbenzene-induced mouse ear edema, carrageenan-induced rat paw edema, acetic acid-induced mouse vascular permeability and cotton pellet-induced rat granuloma tests. RESULTS: Our results demonstrated that taraxasterol dose-dependently attenuated dimethylbenzene-induced mouse ear edema and carrageenan-induced rat paw edema, decreased acetic acid-induced mouse vascular permeability and inhibited cotton pellet-induced rat granuloma formation. CONCLUSION: Our finding indicates that taraxasterol has obvious in vivo anti-inflammatory effects against animal models. It will provide experimental evidences for the traditional use of Taraxacum officinale and taraxasterol in inflammatory diseases.
Assuntos
Anti-Inflamatórios/administração & dosagem , Medicamentos de Ervas Chinesas/administração & dosagem , Edema/tratamento farmacológico , Granuloma/tratamento farmacológico , Esteróis/administração & dosagem , Taraxacum/química , Triterpenos/administração & dosagem , Animais , Permeabilidade Capilar/efeitos dos fármacos , Modelos Animais de Doenças , Edema/fisiopatologia , Granuloma/fisiopatologia , Humanos , Masculino , Camundongos , Ratos , Ratos WistarRESUMO
A novel, sensitive and rapid ultra-performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method for simultaneous quantification of four furostanol glycosides in rat plasma was established and validated. Ginsenoside Rb1 was used as an internal standard. Plasma samples were pretreated by liquid-liquid extraction with n-butanol and chromatographed on a C18 column (2.1×50 mm i.d., 2.6 µm) using a gradient elution program consisting of acetonitrile and water (containing 0.03% formic acid and 0.1 mM lithium acetate) at a flow rate of 0.4 mL/min. Lithium adduct ions were employed to enhance the response of the analytes in electrospray positive ionization mode and multiple reaction monitoring transitions were performed for detection. All calibration curves exhibited good linearity (r>0.999) over the range of 10-20,000 ng/mL for protodioscin and 2-4000 ng/mL for protogracillin, pseudoprotodioscin and pseudoprotogracillin. The recoveries of the whole analytes were more than 80.3% and exhibited no severe matrix effect. Meanwhile, the intra- and inter-day precisions were all less than 10.7% and accuracies were within the range of -8.1-12.9%. The four saponins showed rapid excretion and relative high plasma concentrations when the validated method was applied to the PK study of Dioscorea nipponica extracts by intragastric administration at low, medium and high dose to rats. Moreover, the T(1/2) and AUC(0-t) of each compound turned out to behave in a dose-dependent pattern by comparing them at different dose levels.
Assuntos
Dioscorea , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/farmacocinética , Glicosídeos/sangue , Glicosídeos/farmacocinética , Esteróis/sangue , Esteróis/farmacocinética , Espectrometria de Massas em Tandem/métodos , Administração Oral , Animais , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/administração & dosagem , Glicosídeos/administração & dosagem , Masculino , Ratos , Ratos Wistar , Esteróis/administração & dosagemRESUMO
This paper reflects the position of the CKD-MBD workgroup, an official working group of ERA-EDTA and of the ERBP advisory board, the official guideline-producing body of ERA-EDTA, on the topic of the use of calcimimetics in patients with CKD stage 5D, as based on two recent meta-analysis.
Assuntos
Hiperparatireoidismo Secundário/tratamento farmacológico , Nefrologia/normas , Insuficiência Renal Crônica/complicações , Insuficiência Renal Crônica/tratamento farmacológico , Cinacalcete , Ensaios Clínicos como Assunto , Europa (Continente) , Humanos , Metanálise como Assunto , Naftalenos/administração & dosagem , Fosfatos/química , Sociedades Médicas , Esteróis/administração & dosagem , Resultado do Tratamento , Vitamina D/administração & dosagemRESUMO
The acute and chronic respiratory tract inflammation models were made to investigate the effect and mechanism of sterol extracts from Begonia Sinensis Rhizome (BSR). The first model of acute lung injury was made with Kunming mice by inhaling cigarette smoke, then the mice were treated with different concentrations of BSR sterol extracts. Lung tissue morphology was detected by HE staining, TNF-alpha/MPO were detected by Elisa, and cPLA2 protein were, detected by Western blotting respectively. Results showed that in model group, lung sheet became real, alveolar space shrank or disappeared, alveolar septum was thickened, plenty of inflammatory cells were infiltrated, capillary blood vessels were congestive and the expression of TNF-α, MPO, cPLA2 increased; after administration, a small amount of inflammatory cells were infiltrated, alveolar septum became obvious, capillary congestion status was significantly relieved and the expression of TNF-α, MPO, cPLA2 decreased (P < 0.05). The second model of chronic respiratory tract inflammation in BALB/c mice with bronchial asthma was induced by OVA, then the mice were treated with different concentrations of BSR sterol extracts. Lung tissue morphology was detected by HE staining, indexes such as IL-4, IL-5, IL-13 were detected by Elisa, and the cPLA2 protein expression was detected by Western blotting respectively. Results showed that in model group, a lot of inflammatory cells around lung vessels and bronchi exuded, bronchial goblet cells proliferated and the expression of IL-4, IL-5, IL-13, cPLA2 increased; after administration, inflammatory and goblet cell hyperplasia reduced, the expression of IL-4, IL-5, IL-13, cPLA2 also decreased (P < 0.05). The above results showed BSR sterol extracts could resist against respiratory inflammation by inhibiting cPLA2 in a dose-dependent manner.
Assuntos
Asma/tratamento farmacológico , Begoniaceae/química , Medicamentos de Ervas Chinesas/administração & dosagem , Esteróis/administração & dosagem , Animais , Asma/genética , Asma/imunologia , Citocinas/genética , Citocinas/imunologia , Modelos Animais de Doenças , Feminino , Humanos , Interleucina-13/genética , Interleucina-13/imunologia , Interleucina-4/genética , Interleucina-4/imunologia , Interleucina-5/genética , Interleucina-5/imunologia , Pulmão/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Rizoma/químicaRESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Taraxasterol was isolated from the Chinese medicinal herb Taraxacum officinale which has been frequently used as a remedy for inflammatory diseases. Our previous study has shown that taraxasterol inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 macrophages. To elucidate the underlying mechanism responsible for these effects, in the present study, we investigated the effects of taraxasterol on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, and mitogen-activated protein kinases (MAPKs) signaling pathway in LPS-induced RAW 264.7 macrophages. MATERIALS AND METHODS: RAW 264.7 cells were pretreated with 2.5, 5 and 12.5 µg/ml of taraxasterol 1 h prior to treatment with 1 µg/ml of LPS. The mRNA expression levels of iNOS and COX-2 were examined by RT-PCR. The protein expression levels of iNOS and COX-2, and the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), p38 and c-Jun N-terminal kinase (JNK) MAPKs were measured by Western blot. RESULTS: The mRNA and protein expression levels of iNOS and COX-2 were inhibited by taraxasterol in a concentration-dependent manner. Further studies revealed that taraxasterol suppressed the phosphorylation of ERK1/2 and p38 in LPS-induced RAW 264.7 macrophages. CONCLUSIONS: These results indicate that taraxasterol inhibits iNOS and COX-2 expression by blocking ERK1/2 and p38 MAPKs signaling pathway.
Assuntos
Ciclo-Oxigenase 2/genética , Macrófagos/efeitos dos fármacos , Óxido Nítrico Sintase Tipo II/genética , Esteróis/farmacologia , Triterpenos/farmacologia , Animais , Linhagem Celular , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Macrófagos/metabolismo , Medicina Tradicional Chinesa/métodos , Camundongos , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Óxido Nítrico/metabolismo , Fosforilação/efeitos dos fármacos , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/efeitos dos fármacos , Esteróis/administração & dosagem , Esteróis/isolamento & purificação , Taraxacum/química , Triterpenos/administração & dosagem , Triterpenos/isolamento & purificação , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismoRESUMO
Management of calcium (Ca) and phosphorus (P) metabolism is crucial in chronic hemodialysis (HD) patients. Cinacalcet is usually used for chronic kidney disease-mineral and bone disorders (CKD-MBD) patients with elevated intact parathyroid hormone (iPTH) levels. However, a certain number of CKD-MBD patients have normal iPTH levels and are not subjected to cinacalcet therapy. Here, we evaluated the efficacy of a new treatment algorithm of early initiation of cinacalcet therapy in this subgroup of patients, mainly for correcting Ca and P metabolism. Seventy-one HD patients, including 44 patients without marked elevation of iPTH (102 < iPTH ≤ 300 pg/mL), who received cinacalcet therapy, were enrolled in this study. Serum parameters relating to CKD-MBD patient metabolism, doses of phosphate binders, and type of vitamin D sterols were compared between pre- and post-cinacalcet administration retrospectively. Sixty-four of 71 patients did not require discontinuation of cinacalcet. In these 64 patients, serum Ca (P = 0.0003), P (P = 0.0153), and iPTH (P < 0.0001) levels were significantly reduced after cinacalcet administration, even in those without marked elevation of iPTH (Ca; P < 0.0001, P; P = 0.0422, and iPTH; P = 0.0018). The proportion of patients who received vitamin D sterols was unchanged (P = 0.5930) but the proportion of patients who received maxacalcitol was significantly reduced after cinacalcet administration (P = 0.0108). The new treatment algorithm of early initiation of cinacalcet is considered to be well tolerated and effective for controlling hypercalcemia, and/or hyperphosphatemia and/or increased iPTH of CKD-MBD patients.
Assuntos
Calcimiméticos/uso terapêutico , Naftalenos/uso terapêutico , Hormônio Paratireóideo/sangue , Diálise Renal/métodos , Adulto , Idoso , Algoritmos , Doenças Ósseas Metabólicas/terapia , Calcimiméticos/efeitos adversos , Cálcio/sangue , Cálcio/metabolismo , Cinacalcete , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Naftalenos/efeitos adversos , Fósforo/sangue , Fósforo/metabolismo , Insuficiência Renal Crônica/terapia , Estudos Retrospectivos , Esteróis/administração & dosagem , Fatores de Tempo , Vitamina D/administração & dosagemRESUMO
Temperature-mediated plasticity in life history traits strongly affects the capability of ectotherms to cope with changing environmental temperatures. We hypothesised that temperature-mediated reaction norms of ectotherms are constrained by the availability of essential dietary lipids, i.e. polyunsaturated fatty acids (PUFA) and sterols, as these lipids are involved in the homeoviscous adaptation of biological membranes to changing temperatures. A life history experiment was conducted in which the freshwater herbivore Daphnia magna was raised at four different temperatures (10, 15, 20, 25°C) with food sources differing in their PUFA and sterol composition. Somatic growth rates increased significantly with increasing temperature, but differences among food sources were obtained only at 10°C at which animals grew better on PUFA-rich diets than on PUFA-deficient diets. PUFA-rich food sources resulted in significantly higher population growth rates at 10°C than PUFA-deficient food, and the optimum temperature for offspring production was clearly shifted towards colder temperatures with an increased availability of dietary PUFA. Supplementation of PUFA-deficient food with single PUFA enabled the production of viable offspring and significantly increased population growth rates at 10°C, indicating that dietary PUFA are crucial for the acclimation to cold temperatures. In contrast, cumulative numbers of viable offspring increased significantly upon cholesterol supplementation at 25°C and the optimum temperature for offspring production was shifted towards warmer temperatures, implying that sterol requirements increase with temperature. In conclusion, essential dietary lipids significantly affect temperature-mediated reaction norms of ectotherms and thus temperature-mediated plasticity in life history traits is subject to strong food quality constraints.
Assuntos
Aclimatação/fisiologia , Daphnia/crescimento & desenvolvimento , Gorduras na Dieta/metabolismo , Fenótipo , Temperatura , Animais , Cromatografia Gasosa , Criptófitas , Daphnia/metabolismo , Ácidos Graxos Insaturados/administração & dosagem , Modelos Lineares , Lipossomos , Scenedesmus , Esteróis/administração & dosagemRESUMO
Pluchea sagittalis whole plant dichloromethane extract showed inhibitory activity in several inflammatory models: rat hind paw-edema, mice ear edema, and air-pouch rat granuloma. The extract inhibited the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in stimulated human neutrophils. It also showed inhibitory effect on heat shock protein 72 (hsp72) synthesis in stimulated neutrophils, while it had opposite effects on unstimulated cells. The triterpene taraxasteryl acetate was obtained from the dichloromethane extract by bioassay directed isolation, being active against induced ROS and RNS production in human neutrophils. In mice ear edema (induced by phorbol-12-mirystate-13-acetate, croton oil and arachidonic acid), taraxasteryl acetate showed a topical anti-inflammatory activity similar to the extract, but at 1/20 of the dose. The same ratio was observed for the inhibition of hsp72 production in stimulated human neutrophils. In unstimulated monocytes and neutrophils, taraxasteryl acetate showed a higher stimulating activity of hsp72 production than the extract, involving different mechanisms in each cell type. To our knowledge, taraxasteryl acetate is the first natural product for which a dual effect on the hsp response is reported.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Asteraceae , Edema/prevenção & controle , Granuloma de Corpo Estranho/prevenção & controle , Neutrófilos/efeitos dos fármacos , Fitoterapia , Esteróis/farmacologia , Triterpenos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/uso terapêutico , Ácido Araquidônico , Óleo de Cróton , Relação Dose-Resposta a Droga , Edema/induzido quimicamente , Feminino , Citometria de Fluxo , Proteínas de Choque Térmico HSP72 , Proteínas de Choque Térmico/biossíntese , Proteínas de Choque Térmico/efeitos dos fármacos , Humanos , Masculino , Camundongos , Camundongos Endogâmicos , Neutrófilos/metabolismo , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Estruturas Vegetais , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Espécies Reativas de Nitrogênio/biossíntese , Espécies Reativas de Oxigênio , Esteróis/administração & dosagem , Esteróis/uso terapêutico , Acetato de Tetradecanoilforbol , Triterpenos/administração & dosagem , Triterpenos/uso terapêuticoRESUMO
Three sterols, 5alpha-ergost-7-en-3beta-ol, 5alpha-ergosta-7,22-dien-3beta-ol and 5,8-epidioxy-5alpha,8alpha-ergosta-6,22-dien-3beta-ol and five triterpenes, applanoxidic acids A, C, F, G and H, have been isolated from Ganoderma annulare. The applanoxidic acids A, C and F were found to inhibit the growth of the fungi Microsporum cannis and Trichophyton mentagrophytes at concentrations of 500 to 1000 microg/ml.
Assuntos
Antifúngicos/farmacologia , Ganoderma , Microsporum/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Trichophyton/efeitos dos fármacos , Antifúngicos/administração & dosagem , Antifúngicos/uso terapêutico , Relação Dose-Resposta a Droga , Humanos , Testes de Sensibilidade Microbiana , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Esteróis/administração & dosagem , Esteróis/farmacologia , Esteróis/uso terapêutico , Triterpenos/administração & dosagem , Triterpenos/farmacologia , Triterpenos/uso terapêutico , MadeiraRESUMO
OBJECTIVES: To review use of alternative pharmacotherapy (AP) in patients with cardiovascular disease (CVD) and significant drug interactions between AP and traditional CVD medications. STUDY DESIGN: A literature search of MEDLINE and the National Complementary and Alternative Medicine database was done using these search terms: supplements, vitamins, garlic, fish oil, L-arginine, soy, coenzyme Q10, herbs, phytosterols, chelation therapy, alternative medicine, and CVD. PATIENTS AND METHODS: English human clinical trials measuring surrogate and clinical end points. RESULTS: Antioxidants have not been consistently proven beneficial in reducing cardiovascular mortality. Fish oils may be beneficial in patients with hypertension and hypercholesterolemia, but therapeutic doses need to be defined. Use of coenzyme Q10 in patients with heart failure has not demonstrated consistent benefits. Garlic may lower blood pressure and cholesterol levels, but also may increase bleeding, so its use in CVD patients should be monitored. Clinical studies with small sample sizes have demonstrated that L-arginine may be useful to prevent and treat CVD. The Food and Drug Administration recommends 25 g/day of soy protein as part of a diet low in saturated fats for cholesterol reduction. Plant sterols are recommended by the American Heart Association and the National Cholesterol Education Program Expert Panel as adjunct therapy to reduce low-density lipoprotein. No data support use of chelation therapy. Some APs interact with common prescription CVD medications (eg, gingko and ginseng with warfarin, St. John's Wort with digoxin). CONCLUSIONS: The benefits of APs as part of the treatment for CVD are controversial. Routine use is not recommended.
Assuntos
Fármacos Cardiovasculares/uso terapêutico , Doenças Cardiovasculares/tratamento farmacológico , Suplementos Nutricionais , Preparações de Plantas/uso terapêutico , Ubiquinona/análogos & derivados , Antioxidantes/administração & dosagem , Antioxidantes/uso terapêutico , Arginina/administração & dosagem , Arginina/uso terapêutico , Fármacos Cardiovasculares/efeitos adversos , Doenças Cardiovasculares/prevenção & controle , Ensaios Clínicos como Assunto , Coenzimas , Terapias Complementares , Interações Medicamentosas , Educação Continuada em Farmácia , Óleos de Peixe/administração & dosagem , Óleos de Peixe/uso terapêutico , Alho/efeitos adversos , Humanos , Preparações de Plantas/administração & dosagem , Preparações de Plantas/efeitos adversos , Proteínas de Soja/administração & dosagem , Proteínas de Soja/uso terapêutico , Esteróis/administração & dosagem , Esteróis/uso terapêutico , Ubiquinona/administração & dosagem , Ubiquinona/uso terapêuticoRESUMO
OBJECTIVE: The purpose of this study was to examine effects of a modified soft-ripened cheese containing vegetable oils on cholesterol status, using the rat as the experimental model and the traditional soft-ripened cheese as the control. METHODS: Adult male Wistar rats (approximately 370 g) were divided into two dietary groups (20 rats/group) and fed either the standard diet (STD, containing traditional cheeses made from whole milk) or the experimental diet (EXP, containing modified cheeses made from the combination of skim milk with the following fat mixture: milk fat/oleic acid-enriched sunflower oil/soybean oil mixture). Lipids of the diets came solely from cheeses (14 g/100 g diet); the EXP diet contained (3-fold) less saturated fat, (2-fold) less cholesterol, and (15-fold) more phytosterols than the STD diet. RESULTS: Although serum triglyceride and total cholesterol concentrations were not affected by the type of diet, the EXP diet resulted in a significant reduction of LDL-cholesterol (31%, p < 0.001) and a significant increase of HDL-cholesterol (11%, p < 0.05), compared to the STD diet. Thus, a marked reduction (39%) of serum LDL/HDL cholesterol ratio was observed in the EXP group (p < 0.001). In addition, the two quantitative balances (excreted/ingested) of cholesterol and total neutral sterols (for which phytosterols were excluded) were significantly higher by 183% and 174%, respectively for the EXP group, compared to the STD group (p < 0.05). On another hand, rats fed the EXP diet excreted more cholesterol than they ingested dietary cholesterol (cholesterol balance > 1), indicating that those animals eliminated some endogenous cholesterol in their feces, while the opposite was true for rats fed the STD diet (cholesterol balance < 1). Finally, fecal bile salt concentration was not significantly different between the two dietary groups. CONCLUSIONS: The partial substitution of milk fat by vegetable oils in soft-ripened cheese resulted in a decreased blood LDL/HDL cholesterol ratio and an increased fecal excretion of endogenous cholesterol and neutral sterols and, thus, markedly improved its nutritional qualities. Therefore, the consumption of the described modified cheese may meet the demand of subjects who wish to lower their risk for atherosclerosis and cardiovascular disease.
Assuntos
Queijo/análise , LDL-Colesterol/sangue , Colesterol/sangue , Gorduras na Dieta/administração & dosagem , Óleos de Plantas/farmacologia , Animais , Arteriosclerose/prevenção & controle , Doenças Cardiovasculares/prevenção & controle , LDL-Colesterol/efeitos dos fármacos , Gorduras na Dieta/análise , Ácidos Graxos/administração & dosagem , Ácidos Graxos/análise , Fezes/química , Lipídeos/análise , Lipídeos/sangue , Masculino , Microssomos Hepáticos/química , Modelos Animais , Óleos de Plantas/administração & dosagem , Ratos , Ratos Wistar , Esteróis/administração & dosagem , Esteróis/análiseRESUMO
Through the internal and external experiments of platelet aggregation on rats and rabbits, we study the effects of starfish sterol (No. A1998) on the formation of experimental thrombus in vitro and the effects of platelet aggregation induced by arachidonic acid (AA), ADP, CaCl2. Starfish sterol A 1998 can inhibit the formation of experimental thrombus in vitro of rats on the rates of 10.0%, 20.1%, 25.9% (P < 0.01), p.o. starfish sterol 4.5, 13.5, 40.5 mg/kg. A 1998 can inhibit platelet aggregation induced by AA, ADP, CaCl2 in the same time. It can be concluded that starfish sterol has inhibitory effects on platelet aggregation.
Assuntos
Materia Medica/farmacologia , Inibidores da Agregação Plaquetária/farmacologia , Agregação Plaquetária/efeitos dos fármacos , Estrelas-do-Mar/química , Esteróis/farmacologia , Difosfato de Adenosina , Animais , Ácido Araquidônico , Masculino , Materia Medica/administração & dosagem , Inibidores da Agregação Plaquetária/administração & dosagem , Coelhos , Ratos , Ratos Sprague-Dawley , Esteróis/administração & dosagem , Trombose/induzido quimicamente , Trombose/patologiaRESUMO
A recent study in The Netherlands compared the effects of margarine enriched with different vegetable oil sterols with margarine containing sitostanol-ester on plasma total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol concentrations. Margarine with sterolesters from soybean oil (mainly esters from sitosterol, campesterol, and stigmasterol) was as effective as a margarine with sitostanol-ester in lowering blood total and LDL cholesterol levels without affecting HDL cholesterol levels.
Assuntos
HDL-Colesterol/efeitos dos fármacos , LDL-Colesterol/efeitos dos fármacos , Óleos de Plantas/farmacologia , Esteróis/farmacologia , Adulto , Idoso , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Método Duplo-Cego , Feminino , Humanos , Hidrogenação , Masculino , Margarina , Pessoa de Meia-Idade , Óleos de Plantas/administração & dosagem , Ensaios Clínicos Controlados Aleatórios como Assunto , Esteróis/administração & dosagemAssuntos
Anticolesterolemiantes/administração & dosagem , Anticolesterolemiantes/farmacocinética , Esteróis/farmacocinética , Administração Oral , Animais , Anticolesterolemiantes/sangue , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão , Estudos Cross-Over , Cães , Meia-Vida , Injeções Intra-Arteriais , Masculino , Polietilenoglicóis , Óleo de Cártamo , Esteróis/administração & dosagem , Esteróis/metabolismoRESUMO
The daily intakes of various lipids by 72 Japanese women (40-59 years of age) were measured directly from mock samples of food actually consumed (this method is similar to the duplicate portion method). One sample was collected from each of 12 subjects every 2-months for a period of 1 year. The daily intakes of total fatty acid (FA), cholesterol, plant sterol, phospholipid (PL), and phosphatidylcholine (PC) were 37.9 g, 300 mg, 152 mg, 3.1 g, and 1.7 g, respectively. No effect of the sampling period was found for any lipid measured. The combined eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids showed marked individual variation, and ranged from 0 to 4.3 g, and the average was 0.8 g. The n6/n3 polyunsaturated fatty acids (PUFA) ratio ranged from 0.9 to 19.1 (average, 4.2). There was a strong correlation only between cholesterol and PL intakes (r = 0.796, p < 0.05). The mean serum cholesterol, which was known in 42 subjects was 190 mg/dl, and showed no relation to their daily intakes of any of the measured lipids.
Assuntos
Colesterol/sangue , Gorduras na Dieta/administração & dosagem , Ácidos Graxos/administração & dosagem , Fosfolipídeos/administração & dosagem , Esteróis/administração & dosagem , Adulto , Colesterol na Dieta/administração & dosagem , Ácidos Docosa-Hexaenoicos/administração & dosagem , Ácido Eicosapentaenoico/administração & dosagem , Ácidos Graxos Insaturados/administração & dosagem , Feminino , Humanos , Japão , Pessoa de Meia-Idade , Fosfatidilcolinas/administração & dosagemRESUMO
Twenty-five young couples consumed either a saturated or polyunsaturated fat diet for a 20-week period. The polyunsaturated beef, lamb and dairy products, high in linoleic acid, were produced by feeding ruminant animals "protected lipid" feeds which prevent hydrogenation of fats in the rumen. The experimental design provided for four dietary groups: A) saturated diet for 20 weeks; B) polyunsaturated diet for 20 weeks; C) saturated diet for 10 weeks then polyunsaturated diet for 10 weeks; and D) polyunsaturated for 10 weeks then saturated diet for 10 weeks. Polyunsaturated-to-saturated ratios (linoleic: total saturated fatty acids) for the diets were: prestudy, 0.43-0.56; saturated 0.11-0.14; polyunsaturated, 0.56-0.62. Factors other than diet that appeared to affect cholesterol responses were carefully considered. During the initial 3 weeks (regimentation period) plasma cholesterol concentration decreased in all groups irrespective of diet. This was followed by two experimental periods in which the effects of the prescribed diets were readily apparent (response periods I and II). The final 4 weeks of the study were characterized by diminishing compliance with the dietary discipline (abatement period). Subjects in each group (A,B,C,D) were subdivided into high and low on a basis of each group's initial median cholesterol level. Those in the high subgroups were very responsive to dietary changes whereas those in the low subgroups were not. Combined responses of high subjects were: polyunsaturated diet, -10.7 mg/dl (P less than 0.025), saturated diet, d8 mg/dl (P less than 0.05). Combined difference between saturated and polyunsaturated diets was 18.5 mg/dl or approximately a 10% cholesterol difference between the experimental polyunsaturated and saturated diets.