RESUMO
OBJECTIVE: To explore the Kidney-tonifying and abortion preventing effect of Shou Tai Wan (STW) by different extration methods on the SD Rats' abortion model. METHODS: Applied hydroxycarbamide and mifepristone (RU-486) to establish the abortion model of corpus luteum inhibition due to Kidney deficiency (disease-syndorme combination model) on SD, rats. Treated the model rats with STW formula. Observed the uterus condition and recorded the embryo number and the miscarriage rate of each rat. 4 kinds of extractions including water extract of STW (A liquid), alcohol extract of STW (B liquid), after the ethanol water extract residue of STW (C liquid) and B + C liquid. Visual observed the uterine lesions embryos and calculated obortion rate. Used chemluninescence methed to cheek the serum estradiol (E2) and progesterone (P) level. Used quantitative RT-PCR (qRT-PCR) to analyze the different of the PR mRNR between the model group and the treated group. RESULTS: Compared with the model group, the abortion rate of B + C liquid was greatly deduced and the embryo number of B + C liquid group, the E2 and P levels were obviously increased in the treated groups. CONCLUSION: STW (B + C) has the best effect of tonifying the kidney and preventing abortion.
Assuntos
Aborto Espontâneo/prevenção & controle , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Nefropatias/prevenção & controle , Aborto Espontâneo/sangue , Aborto Espontâneo/induzido quimicamente , Animais , Modelos Animais de Doenças , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/administração & dosagem , Estradiol/sangue , Feminino , Nefropatias/sangue , Nefropatias/induzido quimicamente , Masculino , Plantas Medicinais/química , Gravidez , Progesterona/sangue , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Fármacos Renais/administração & dosagem , Fármacos Renais/isolamento & purificação , Fármacos Renais/farmacologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Útero/efeitos dos fármacos , Útero/metabolismoRESUMO
A water-soluble polysaccharide (CPS-2), isolated from the cultured Cordyceps sinensis, was obtained by hot-water extraction, anion-exchange and gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, periodate oxidation, methylation analysis, FTIR and NMR spectroscopy. CPS-2 was found to be mostly of alpha-(1-->4)-D-glucose and alpha-(1-->3)-D-mannose, branched with alpha-(1-->4,6)-D-glucose every twelve residues on average. CPS-2 had a molecular weight of 4.39x10(4) Da. The protective effect of CPS-2 on the model of chronic renal failure was established by fulgerizing kidney. The changes in blood urea nitrogen and serum creatinine revealed that CPS-2 could significantly relieve renal failure caused by fulgerizing kidney.