RESUMO
BACKGROUND: The incidence of allergy has been increasing at an alarming rate over the last few decades. OBJECTIVE: Our present study aims to find out the structurally homologous motifs present in different proteinaceous allergens. METHODS: Significant number of protein sequences and their corresponding structures of various pollen, fungal, bacterial, and food allergens were retrieved and the sequence and structural identity were analyzed. RESULTS: Intra- and inter-sequence along with their structural analysis of the proteinaceous allergens revealed that no significant relationships exist among them. A few, but not the negligible number of high structural similarities, were observed within different groups of allergens from fungus, angiosperms, and animals (Aves and Mammalia). CONCLUSION: Our in silico study on thirty-six different allergens showed a significant level of structural similarities among themselves, regardless of their sequences.
Assuntos
Alérgenos/química , Fluprednisolona/análogos & derivados , Proteínas de Plantas/química , Sequência de Aminoácidos , Animais , Bactérias/química , Simulação por Computador , Bases de Dados Factuais , Fluprednisolona/química , Hipersensibilidade Alimentar/etiologia , Fungos/química , Humanos , Imunoglobulina E/metabolismo , Filogenia , Pólen/química , Conformação ProteicaRESUMO
Preparation of oil-in-water (o/w) type lipid emulsion is one of the approaches to formulate drugs that are poorly water-soluble but can be dissolved in the oil phase of the emulsions. A synthetic glucocorticoid medicine, difluprednate (DFBA), is a water-insoluble compound. We formulated DFBA (0.05%, w/v) ophthalmic lipid emulsion containing 5.0% (w/v) caster oil and 4.0% (w/v) polysorbate 80. The appearance of the emulsion was blue and translucent lipid emulsion, and the median particle size of the lipid emulsion was 104.4 nm. Neither separation nor change in particle size was observed after 6 months at 40 degrees C. Furthermore, when compared with DFBA (0.05%, w/v) ophthalmic suspension, the lipid emulsion showed 5.7-fold higher concentration of DFB that was an active metabolite of DFBA in aqueous humor at 1h after instillation. Ophthalmic lipid emulsion enhances the intraocular penetration of drugs, and it is useful as a delivery system for the ophthalmic preparations of lipophilic drugs.