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1.
Cell Mol Biol (Noisy-le-grand) ; 62(3): 72-7, 2016 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-27064876

RESUMO

The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Neoplasias do Colo/tratamento farmacológico , Doxorrubicina/farmacologia , Flavonoides/farmacologia , Morus/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Apoptose/efeitos dos fármacos , Colo/efeitos dos fármacos , Colo/metabolismo , Colo/patologia , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Doxorrubicina/química , Flavonoides/química , Flavonoides/isolamento & purificação , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Genes APC/efeitos dos fármacos , Células HT29 , Humanos , Extratos Vegetais , Folhas de Planta/química , Poli(ADP-Ribose) Polimerases/análise
2.
Biochem Biophys Res Commun ; 430(2): 623-8, 2013 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-23219837

RESUMO

Folate, one of the most studied dietary compounds, has recently become the main topic of debates on food fortification. Although low folate levels may be associated with increased risk of cancer development, simultaneously several reports indicate a detrimental effects mediated by high folate concentrations. Using the methylation sensitive restriction analysis (MSRA) and real-time RT-PCR we tested the effect of folic acid on DNA promoter methylation and expression of PTEN, APC and RARbeta2 tumour suppressor genes in MCF-7 and MDA-MB-231 breast cancer cell lines with different invasive capacity. The tested genes encode proteins involved in regulation of oncogenic intracellular signaling pathways. The results show that the increasing concentrations of folic acid lead to a dose-dependent down-regulation of tumour suppressor genes which may be linked to the increased DNA methylation detected within their promoter regions. The effects were more remarkable in non-invasive MCF-7 cells where we also observed 30% up-regulation of DNMT1 expression at the highest folate concentration used. Our findings show that caution need to be used when introducing folic acid supplementation since it may lead to cancer progression.


Assuntos
Neoplasias da Mama/patologia , Metilação de DNA/efeitos dos fármacos , Ácido Fólico/farmacologia , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Inativação Gênica , Genes APC/efeitos dos fármacos , Genes Supressores de Tumor/efeitos dos fármacos , PTEN Fosfo-Hidrolase/genética , Receptores do Ácido Retinoico/genética , Linhagem Celular Tumoral , DNA (Citosina-5-)-Metiltransferase 1 , DNA (Citosina-5-)-Metiltransferases/biossíntese , Progressão da Doença , Feminino , Humanos , Mapeamento por Restrição
3.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 18(4): 866-72, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-20723290

RESUMO

This study was aimed to investigate the effect of traditional Chinese medicine, Triptolide (TPL) on reversing hypermethylation of antioncogene (apc gene) in acute lymphoblastic leukemia cell line Jurkat in vitro and to explore its mechanisms. The effects of TPL on cell growth, proliferation and cell cycle were detected by growth curve, MTT assay, colony formation test and flow cytometry, respectively. The effect of TPL on apc gene methylation of Jurkat cells was analyzed by nested methylation specific PCR; the expressions of apc gene, dnnt3a, dnmt3b mRNA were measured by RT-PCR; the protein expression of apc gene was detected by Western blot. The results showed that as compared with untreated control cells, the TPL of different concentrations could significantly inhibit growth and proliferation of Jurkat cells in dose-and time-dependent manners with IC50 19.7 ng/ml at 48 hours. All cytosines in CpG dinucleotides in untreated Jurkat cells had no changed, while all cytosines in Jurkat cells treated with TPL had been converted to thymidine suggesting the methylation of apc gene in Jurkat cells. The TPL could reverse hypermethylation of apc gene and induced the mRNA and protein expression of apc gene in dose-dependent manner. It is concluded that the small dose of TPL can obviously suppress the proliferation of Jurkat cells, activate and up-regulate the expression of apc gene through demethylation of apc gene resulting from DNMT and/or direct action, thereby inhibit the proliferation rate of Jurkat cells.


Assuntos
Antineoplásicos Alquilantes/farmacologia , Metilação de DNA/efeitos dos fármacos , Diterpenos/farmacologia , Genes APC/efeitos dos fármacos , Fenantrenos/farmacologia , Proliferação de Células/efeitos dos fármacos , DNA (Citosina-5-)-Metiltransferases/metabolismo , DNA Metiltransferase 3A , Compostos de Epóxi/farmacologia , Regulação Leucêmica da Expressão Gênica , Humanos , Células Jurkat , DNA Metiltransferase 3B
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