RESUMO
With an aim of producing commercially important tannase enzyme using cheap and readily available agro-residues, leaves of Indian Gooseberry (Phyllanthus emblica) and Jamun (Syzygium cumini), peels of Lemon (Citrus limon), and Pomegranate (Punica granatum) were screened. Newly isolated Geotrichum cucujoidarum was utilized for the study. Preliminary studies indicated that tannase titer obtained is not proportional to the tannin content of the agro-residues and solid state fermentation superior compared to submerged fermentation. Jamun mixed with lemon peel in equal proportion supplemented with minerals under solid-state fermentation gave a tannase titer of 15.46 U/g dry solids. Through successful implantation of Plackett-Burman design, yeast extract concentration, inoculum volume, and amount of substrate were found to be the most significant factors. Further optimization of these three factors through Response Surface Methodology resulted in the 1.7-fold increase in tannase titer. Validation experiments using 3.97 g of Jamun leaves + lemon peel powder mixed with a nutrient solution having (w/v) yeast extract - 1.1%, dextrose - 3%, Urea - 1.125%, potassium chloride - 0.1%, magnesium sulfate heptahydrate - 0.1% with the initial pH of 5, inoculated with 2.48 ml of inoculum gave a tannase titer of 26.43 U/g dry solids after 6 days of solid-state fermentation.
Assuntos
Dipodascus , Geotrichum , Taninos , Fermentação , Hidrolases de Éster Carboxílico/químicaRESUMO
BACKGROUND: Mold-ripened cheeses have low levels of unsaturated fatty acids (UFAs). Geotrichum candidum is an adjunct culture for the development of Geotrichum-ripened cheese but has a low ability to produce high levels of UFAs. Δ12 fatty acid desaturase (FADS12) is a pivotal enzyme that converts oleic acid (OA) to linoleic acid (LA) and plays a vital role in UFA biosynthesis. By investigating FADS12 catalytic activity from various species with OA substrates, we found that FADS12 from Mucor circinelloides (McFADS12) had the highest catalytic activity for OA. RESULTS: In the current study, a plasmid harboring McFADS12 was constructed and overexpressed in G. candidum. Our results showed that LA production increased to 31.1 ± 1.4% in engineered G. candidum - three times higher than that in wild-type G. candidum. To enhance LA production, an exogenous substrate (OA) was supplemented, and the yield of LA was increased to 154 ± 6 mg L-1 in engineered G. candidum. Engineered G. candidum was used as an adjunct culture for Geotrichum-ripened cheese production. The LA level reached 74.3 ± 5.4 g kg-1 cheese, whereas the level of saturated fatty acids (SFAs) decreased by 9.9 ± 0.5%. In addition, the soybean byproduct (okara) was introduced into the engineered G. candidum growth and the level of LA increased to 126 ± 4 g kg-1 cheese and the percentage of UFAs:SFAs increased from 0.8:1 to 1.3:1. CONCLUSION: This study offers a suitable technology for converting SFAs to UFAs in Geotrichum-ripened cheeses and provides a novel trend for converting soybean waste into a value-added product. © 2022 Society of Chemical Industry.
Assuntos
Queijo , Ácidos Graxos Dessaturases , Geotrichum , Farinha , Ácido LinoleicoRESUMO
Trichosporon fermentans can be used to treat refined soybean oil wastewater (RSOW) and produce microbial lipids. Bioflocculation is an effective method to recover Trichosporon fermentans which accumulates intracellular oils from wastewater. During the flocculation, the hydrodynamic distribution and parameters in the reactor are important limiting factors of yeast flocculation performance. In a 0.25 L flocculation device, it was found that the appropriate range of turbulence kinetic energy was within 0.00065-0.00073 m2/s2, the dissipation rate was within 0.119-0.317 m2/s3, and the shear force was less than 0.433 Pa by computational fluid dynamics. In this case, the flocculation rate (Fr) of Trichosporon fermentans could reach more than 90%. The empirical formula associated Fr of Trichosporon fermentans with hydrodynamic parameters was obtained by Matlab, and improved in the enlargement of flocculation device, displaying an error of less than 3.03%. A conical draft tube airlift circulating reactor for flocculation was designed based on the empirical formula, and the Fr reached 91.3%. The study shows that it is feasible to predict Fr of Trichosporon fermentans according to hydrodynamic parameters by numerical simulation, and design the industrial reactor for flocculation harvesting yeasts. It is also helpful for large-scale treatment of RSOW in a safe environment.
Assuntos
Trichosporon , Águas Residuárias , Floculação , Geotrichum , Hidrodinâmica , Óleo de SojaRESUMO
BACKGROUND: Two edible coating (EC) emulsions based on potato starch (F6 and F10) alone or formulated with sodium benzoate (SB, 2% w/w) (F6/SB and F10/SB) were evaluated to maintain postharvest quality of cold-stored 'Fino' lemons and control sour rot on lemons artificially inoculated with Geotrichum citri-aurantii. Previous research showed the potential of these ECs to improve the storability of 'Orri' mandarins and reduce citrus green and blue molds caused by Penicillum digitatum and Penicillium italicum, respectively. RESULTS: The coatings F6/SB and F10/SB significantly reduced sour rot incidence and severity compared to uncoated control samples on lemons incubated at 28 °C for 4 and 7 days. The F6/SB coating reduced weight loss and gas exchange compared to uncoated fruit after 2 and 4 weeks of storage at 12 °C plus a shelf life of 1 week at 20 °C, without adversely affecting the lemon physicochemical quality. CONCLUSION: Overall, the F6/SB coating formulation, composed of pregelatinized potato starch, glyceryl monostearate, glycerol, emulsifiers and SB, with a total solid content of 5.5%, showed the best results in reducing citrus sour rot and maintaining the postharvest quality of cold-stored 'Fino' lemons. Therefore, it showed potential as a new cost-effective postharvest treatment suitable to be included in integrated disease management programs for citrus international markets with zero tolerance to chemical residues. © 2021 Society of Chemical Industry.
Assuntos
Citrus/microbiologia , Conservação de Alimentos/métodos , Fungicidas Industriais/química , Fungicidas Industriais/farmacologia , Geotrichum/efeitos dos fármacos , Doenças das Plantas/microbiologia , Amido/química , Amido/farmacologia , Citrus/química , Composição de Medicamentos , Armazenamento de Alimentos , Frutas/química , Frutas/microbiologia , Geotrichum/crescimento & desenvolvimento , Doenças das Plantas/prevenção & controle , Benzoato de Sódio/química , Solanum tuberosum/químicaRESUMO
Single cell oils (SCO) are oils derived from microorganisms which have potential to hyperaccumulate intracellular lipids (called oleaginous) under some essential nutrient (nitrogen, phosphorous or sometimes sulphur) starvation and an excess of carbon. The present work investigates the influence of these key parameters (for triggering oleaginicity), i.e. carbon (C) and nitrogen (N) on oleaginous behaviour of an oleaginous isolate, with the objective of improving the lipid content and obtaining oils of applicative interest. Eleven yeasts were isolated from rotten fruits and a unique yeast from rotten apple was screened on the basis of its ~ 20% (of dry mass) lipid content (LC), trademark of oleaginicity under nitrogen-stressed culture conditions. Subsequent investigation on influence of C, N and w/w ratio of carbon source concentration (Cs) to nitrogen source concentration (Ns) was conducted on this isolate. The isolate was identified as a Deuteromycete-Geotrichum candidum. 4.8 g/l was found to be minimum N concentration and glucose as suitable C source for optimum balance between biomass and lipid content. The highest LC of 73.6% (172.5% higher compared to 27% LC at Cs/Ns 80/4.8) was obtained at Cs/Ns 150/4.8 with a lipid coefficient of 8.7 (g lipid/100 g substrate). While remarkably higher production economy (lipid coefficient of 28.45) was noted at Cs/Ns 100/4.8 with significant LC of 54.4% (~ 100% higher than at Cs/Ns 80/4.8). The derived oils were predominantly rich in medium-chain fatty acids (MCFA)-caprylic acid, rare in plant oils. G. candidum is a previously referred oleaginous species; however, for the first time this study illustrates its detailed oleaginous behaviour and lipid compositional characteristics with varying nutritional parameters. The work is a progressive contribution towards current and upcoming researches in field of SCOs. Compositional characteristics of derived oils, make it an important candidate for potential medical and nutritional applications in future.
Assuntos
Ácidos Graxos , Geotrichum , Lipídeos , Óleos de PlantasRESUMO
Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L-1 min-1 in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L-1 min-1, using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min-1 at 45 °C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC-MS as γ-octalactone and γ-nonalactone.
Assuntos
Enzimas Imobilizadas/química , Geotrichum/crescimento & desenvolvimento , Lipase/química , Óleos de Plantas/metabolismo , Sementes/química , Vitis/química , Compostos Orgânicos Voláteis/metabolismo , Hidrólise , Óleos de Plantas/químicaRESUMO
Geotrichum candidum is a filamentous fungus mostly used in the dairy industry for cheese ripening and flavoring. The current study was designed to evaluate the potential probiotics effect of dietary supplementation of G. candidum on growth, digestive enzymes activities, gut microbiota and immune-related gene expression and disease resistance in Gibel carp CAS â ¢ (Carassius auratus gibelio) against A. hydrophila infections. For this purpose, 420 healthy fish (initial body weight: 7.09 ± 0.02g) were randomly divided into 7 feeding groups in triplicates of 20 fish in each tank. Seven isocaloric and isonitrogenous diets were prepared, supplemented with 6 different doses of G. candidum viz; C: 0, T1:106, T2:107, T3:108, T4:109, T5:1010, T6:1011 CFU/kg diet and fed to fish twice a day for 60 days. Results showed significantly higher weight gain (WG) and feed efficiency (FE) in groups fed 106 and 108 CFU/kg diet compared to the control (P < 0.05). All G. candidum fed groups showed higher intestinal α-amylase activity (P < 0.05) while no difference in lipase and trypsin activity compared to the control group. Before challenge test, gut microbial diversity analysis revealed relatively more abundance of bacteria belonging to phylum Proteobacteria, Actinobacteria, Firmicutes and Bacteriodetes in group fed G. candidum supplemented diet and higher percentage of pathogenic bacteria belonging to order Aeromonadales and Vibrionales in control post-challenge. After challenge test immune response expressed numerically higher survival rate (P > 0.05) and significantly higher (P < 0.05) Respiratory Burst Activity(RBA), Immunoglobulin M level (IgM), Aspartate Transaminase activity (AST) and improved (P > 0.05) lysozyme activity, and Alanine Transaminase activity (ALT) in groups fed 106 and 108 CFU/kg G. candidum supplemented diet. The expression of immune related gene (IL-I ß, TNF- α, HSP70 and TLR-2) in liver of the fish were significantly affected (P < 0.05) by supplementation of G. candidum post bacterial challenge. The results showed that G. candidum is a potential probiotic as it could improve feed utilization, immunity and cause no harmful effects on growth of gibel carp at higher supplementation levels. The productive results can be achieved by using the lower dose as 106-108 CFU/kg diet.
Assuntos
Suplementos Nutricionais , Resistência à Doença , Microbioma Gastrointestinal , Geotrichum/fisiologia , Carpa Dourada/imunologia , Infecções por Bactérias Gram-Negativas/veterinária , Probióticos/administração & dosagem , Aeromonas hydrophila , Ração Animal , Animais , Citocinas/imunologia , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Carpa Dourada/genética , Carpa Dourada/crescimento & desenvolvimento , Infecções por Bactérias Gram-Negativas/prevenção & controle , Intestinos/microbiologiaRESUMO
Encapsulated probiotic administration can be a nutritional strategy to improve the growth performance and immune status of fish. Here commercial application of encapsulated G. candidum was evaluated as a feed supplement to fingerlings of L. rohita reared in earthen ponds under semi-intensive culture conditions. Fingerlings with an average body weight of 20⯱â¯2.34â¯g were distributed randomly in three groups and experiment was conducted in triplicate. The control group (P0) was fed 35% protein basal diet while the two treated groups, P1 and P2 were fed basal diet supplemented with 109â¯CFUâ¯g-1 un-encapsulated (free) and encapsulated G. candidum, respectively, for eleven weeks. Results indicated significantly (Pâ¯<â¯0.05) improved growth rate, intestinal enzyme activities (protease, amylase and cellulase) and hemato-immunological indices (RBCs, Hb, HCT, WBCs, MCHC, respiratory bursts and phagocytic activity, total protein, lysozyme, IgM), upregulation of heat shock protein HSP 70 gene in muscle, intestine and liver tissues and reduction of serum AST and ALT activities, total cholesterol and triglyceride in fish fed G. candidum supplemented diets (P1 and P2 groups) as compared to basal diet (P0 group). However, diet supplemented with encapsulated G. candidum showed the most significant (pâ¯<â¯0.001) positive effect in comparison to un-encapsulated probiotic. In conclusion, a pronounced effect of G. candidum especially in the encapsulated form on the growth, health status and immunity of L. rohita reared in semi intensive culture system, suggesting its application as a feed additive in practical/commercial semi-intensive earthen pond culture system.
Assuntos
Ração Animal , Cyprinidae/crescimento & desenvolvimento , Cyprinidae/imunologia , Suplementos Nutricionais , Geotrichum , Probióticos/administração & dosagem , Animais , Aquicultura/métodos , Imunidade InataRESUMO
Sour rot is a leading disease of citrus fruit caused by the postharvest pathogen Geotrichum citri-aurantii. It has been reported that essential oils can be used as substitutes for synthetic fungicides to control the pathogen. In this study, changes in metabolites and antifungal effects of G. citri-aurantii treated with peppermint oil (PO) were investigated. The inhibition rate of the mycelial growth increased as the PO concentration increased, and 6 µl PO/disk resulted in a radial growth inhibition of 79.2%. The electrical conductivity of G. citri-aurantii treated with PO increased compared to the control. By comparing the metabolic profiles of treated and untreated G. citri-aurantii cells, a total of 53 distinct metabolites 9 were up-regulated and 44 were down-regulated were found, including 16 lipid metabolites, 6 carbohydrate metabolites, 2 amino acid metabolites, 5 alcohols, 2 glycoside metabolites, and 3 ketone metabolites, etc, and these metabolites are involved in 25 major metabolic pathways. PRACTICAL APPLICATIONS: Chemical fungicides can effectively control G. citri-aurantii during fruit postharvest period. However, synthetic chemical fungicides have gradually led to buildup of resistance of fungil, which seriously causes the frequent of food-borne diseases. PO extracted from natural plants can be used as natural additive in many foods due to their antioxidant, antibacterial, and antifungal properties. Therefore, PO can be considered as a promising bacteriostatic agent for the defense of G. citri-aurantii during fruit postharvest period.
Assuntos
Proteínas Fúngicas/genética , Fungicidas Industriais/farmacologia , Geotrichum/química , Geotrichum/efeitos dos fármacos , Óleos de Plantas/farmacologia , Cromatografia Líquida de Alta Pressão , Citrus/microbiologia , Proteínas Fúngicas/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Regulação Fúngica da Expressão Gênica/efeitos dos fármacos , Geotrichum/genética , Geotrichum/metabolismo , Mentha piperita , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controleRESUMO
This study aimed to optimize free fatty acid production by enzymatic hydrolysis of cottonseed, olive and palm kernel oils in stirred-tank reactors using a lipase from Geotrichum candidum (GCL-I). The effect of pH, temperature and substrate concentration on the hydrolytic activity of GCL-I using these vegetable oils was investigated. Thermal stability tests and thermodynamic studies were also performed. A complete hydrolysis of cottonseed oil was obtained after 120 min of reaction, while for olive and palm kernel maximum hydrolysis percentage was 96.4% and 60.1%, respectively. GCL-I exhibited the highest activity in the hydrolysis of vegetable oils that are rich in unsaturated-fatty acids (cottonseed and olive oils). Under optimal conditions (46.8% m/m of oil, 6.6 U/g of the reaction mixture at 40 °C), complete cottonseed oil hydrolysis was observed at 60 min of reaction performed in an emulsifier-free system with no buffer.
Assuntos
Ácidos Graxos/química , Proteínas Fúngicas/química , Geotrichum/enzimologia , Lipase/química , Óleo de Palmeira/química , HidróliseRESUMO
BACKGROUND: Fungal infection with opportunistic fungi can cause a serious problem for immunocompromised persons such as organ-transplant recipients, cancer, and HIV/AIDS patients. Control of these organisms using natural products is an interesting strategy to avoid the use of heavy chemotherapy in patients. OBJECTIVE: This study aimed to use the extract of Forsskaolea tenacissima L. and Xanthium spinosum L. to suppress the growth of Candida albicans and Geotrichum candidum and to investigate their potential mode of action. MATERIALS AND METHODS: Different plant extracts were tested for their antifungal activity using disc diffusion method and their mode of action was explored using the scanning electron microscopy (SEM) and gas chromatography-mass spectrometry (GC-MS). RESULTS: The results showed that chloroform extract of X. spinosum was the most effective against G. candidum, inhibiting its growth at very low concentration (38µg/mL). Chloroform extract of F. tenacissima was the most effective against C. albicans, with a minimum inhibitory concentration of 39µg/mL. SEM demonstrated the fungitoxicity of the plant extracts against both pathogens. C. albicans treated with plant extract were invaginated and ruptured and the treated mycelia of G. candidum were distorted and squashed. GC-MS analysis showed that the chloroform extract of both plants had 13 different compounds. CONCLUSION: Due to these promising results, these extracts should be further investigated and tested on different strains of C. albicans and G. candidum towards validation of their efficacy as a natural drug.
Assuntos
Antifúngicos/farmacologia , Candida albicans/efeitos dos fármacos , Geotrichum/efeitos dos fármacos , Extratos Vegetais/farmacologia , Antifúngicos/química , Candida albicans/ultraestrutura , Candidíase/microbiologia , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Micélio/efeitos dos fármacos , Infecções Oportunistas/microbiologia , Extratos Vegetais/química , Folhas de Planta/química , Caules de Planta/química , Xanthium/químicaRESUMO
We experienced a breakthrough fungal infection caused by Geotrichum capitatum during empirical therapy with caspofungin. A 68-year-old male patient with refractory acute lymphoblastic leukemia had received umbilical cord blood transplantation after two courses of induction therapy. Empirical therapy with caspofungin was initiated 5 days before transplantation. Tacrolimus was continuously infused to prevent graft-versus-host disease. A minidose of methotrexate was intravenously administered on days 1 and 3 post-transplantation, which was changed to prednisolone from day 7 due to severe mucositis. During a recurrence of fever on day 11, blood cultures were found to be positive for a yeast-like organism, which was later confirmed by mass spectrometry to be G. capitatum. The serum levels of beta-D-glucan were elevated to 747 pg/mL. Caspofungin was switched to liposomal amphotericin B; however, radiological findings revealed pulmonary, splenic, and central nervous system involvement. Progressive renal and hepatic dysfunction subsequently developed. The patient died on day 25 post-transplantation secondary to the development of hemophagocytic syndrome and respiratory failure. We emphasize that recurrent febrile episodes, prolonged neutropenia, and underlying gastrointestinal mucosal damage require extreme caution due to the possibility of breakthrough infection caused by new fungal pathogens during empirical therapy with caspofungin.
Assuntos
Antifúngicos/uso terapêutico , Caspofungina/uso terapêutico , Transplante de Células-Tronco de Sangue do Cordão Umbilical/efeitos adversos , Geotrichum , Micoses/tratamento farmacológico , Micoses/etiologia , Idoso , Hemocultura , Evolução Fatal , Violeta Genciana , Geotrichum/efeitos dos fármacos , Humanos , Masculino , Testes de Sensibilidade Microbiana , Microscopia , Micoses/diagnóstico , Fenazinas , Leucemia-Linfoma Linfoblástico de Células Precursoras/complicações , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapiaRESUMO
In this work, we investigated the in vitro and in vivo antifungal activity of aqueous extracts obtained from eight Cistus plant species against the development of Geotrichum citri-aurantii, the causal agent of citrus sour rot. The results demonstrate the in vitro effectiveness of all tested Cistus species aqueous extracts against G. citri-aurantii, the inhibition of mycelial growth ranged between 80 and 100%. Furthermore, Cistus aqueous extracts totally inhibited germination of G. citri-aurantii arthrospores at a concentration of 5 mg/mL. Among the plant species tested, C. laurifolius, C. salviifolius, C. monspeliensis, C. ladanifer and C. populifolius displayed the best fungistatic activity since the minimum inhibitory concentration (MIC) was <0.625 mg/mL. Under in vivo conditions, incidence of sour rot was lowered to 8.33% when fruits were treated with aqueous extracts of C. populifolius and C. creticus compared to 100% in the control. Disease severity was lowered to 3.74, 4.47 and 5.13% when fruits were treated with C. creticus, C. ladanifer and C. populifolius aqueous extracts, respectively. Using such these biopesticides in a replacement for synthetic fungicides or in combination with other established disease management practices could help control citrus postharvest decay in a more sustainable and eco-friendly way.
Assuntos
Cistus/química , Citrus/microbiologia , Fungicidas Industriais/farmacologia , Geotrichum/efeitos dos fármacos , Doenças das Plantas/microbiologia , Extratos Vegetais/farmacologia , Fungicidas Industriais/isolamento & purificação , Geotrichum/crescimento & desenvolvimento , Hifas/efeitos dos fármacos , Hifas/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Doenças das Plantas/prevenção & controle , Extratos Vegetais/isolamento & purificação , Esporos Fúngicos/efeitos dos fármacos , Esporos Fúngicos/crescimento & desenvolvimentoAssuntos
Antifúngicos/uso terapêutico , Celulite (Flegmão)/microbiologia , Dermatomicoses/tratamento farmacológico , Dermatomicoses/microbiologia , Gastrostomia/efeitos adversos , Geotricose/tratamento farmacológico , Geotricose/microbiologia , Geotrichum , Infecção da Ferida Cirúrgica/tratamento farmacológico , Idoso de 80 Anos ou mais , Anfotericina B/uso terapêutico , Antifúngicos/farmacologia , Aspirina , Combinação de Medicamentos , Feminino , Flucitosina/uso terapêutico , Geotrichum/efeitos dos fármacos , Humanos , Óxido de Magnésio , Testes de Sensibilidade Microbiana , Infecção da Ferida Cirúrgica/microbiologiaRESUMO
The paper presents the chemical characteristics of ellagitannins isolated from raspberry (Rubus idaeus L.) fruit and their in vitro and in situ antifungal activity against Geotrichum candidum LOCK 0511. The study investigated a complex preparation containing various raspberry ellagitannins at a concentration of 86% w/w, as well as pure lambertianin C and sanguiin H-6. The ellagitannin preparation was obtained by extracting raspberry press cake and purifying the extract using Amberlite XAD resin, while individual compounds were isolated by means of preparative HPLC. The complex preparation was analyzed for the content of ellagitannins, anthocyanins, and flavan-3-ols using HPLC and LC-MS. The antifungal activity of the complex ellagitannin preparation and the isolated ellagitannins was determined for the strain Geotrichum candidum. The MIC and MFC values (10.0 mg/mL and 30.0 mg/mL, respectively) were found to be the same for lambertianin C, sanguiin H-6, and the complex ellagitannin preparation. The fungistatic activity of the studied ellagitannin preparation at a concentration of 10 mg/mL, as determined by the poisoned medium method, was 65.2% following 6 day incubation of Geotrichum candidum, with the linear growth rate of only 16.2 mm/day. The corresponding parameters for the control sample were 0% and 56 mm/day, respectively. The study demonstrated both in vitro and in situ antifungal activity of raspberry ellagitannins against Geotrichum candidum.
Assuntos
Antifúngicos/farmacologia , Produtos Biológicos/farmacologia , Frutas/química , Geotrichum/efeitos dos fármacos , Taninos Hidrolisáveis/farmacologia , Extratos Vegetais/farmacologia , Rubus/química , Antifúngicos/química , Produtos Biológicos/química , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Taninos Hidrolisáveis/química , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Extratos Vegetais/químicaAssuntos
Antifúngicos/uso terapêutico , Claritromicina/uso terapêutico , Fungemia/tratamento farmacológico , Geotricose/tratamento farmacológico , Geotrichum , Antibacterianos/uso terapêutico , Fungemia/microbiologia , Geotricose/microbiologia , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Infecções por Pseudomonas/complicações , Infecções por Pseudomonas/tratamento farmacológico , Infecções por Pseudomonas/microbiologia , Superinfecção/tratamento farmacológico , Superinfecção/microbiologiaRESUMO
UNLABELLED: The effectiveness of methanol and chloroform extracts of eight Cistaceae species to control citrus sour rot decay, caused by Geotrichum citri-aurantii, was investigated in both in vitro and in vivo conditions. Methanol extracts of these plant species exhibited more interesting activity against G. citri-aurantii, in both in vitro and in vivo conditions, compared with chloroforme extracts. Under in vitro trials, obtained results showed that methanol extracts of all tested plants revealed a highest significant antifungal activity with inhibition zones that ranged between 12·33 and 16·33 mm in diameter. All tested methanol extracts totally inhibited spore germination when tested at 10 mg ml(-1) . Incidence of sour rot was significantly lowered to 11·11% when fruits were treated with Cistus populifolius and Cistus ladanifer methanol extracts compared with 100% in the control. The disease severity was lowered to 5·19% and 6·04% when fruits were treated with the same methanol extracts respectively. SIGNIFICANCE AND IMPACT OF THE STUDY: The methanol Cistus extracts had sufficient antifungal activities in vitro and in vivo against G. citri-aurantii to consider its use in the citrus industry after it has been tested under production and natural infection conditions. Such natural products therefore represent a viable alternative approaches for sour rot postharvest management of citrus.
Assuntos
Antifúngicos/farmacologia , Cistus/metabolismo , Citrus/microbiologia , Geotrichum/efeitos dos fármacos , Doenças das Plantas/terapia , Extratos Vegetais/farmacologia , Clorofórmio/química , Frutas/microbiologia , Metanol/química , Testes de Sensibilidade Microbiana , Doenças das Plantas/microbiologiaRESUMO
Background. Saprochaete capitata (formerly known as Geotrichum capitatum and Blastoschizomyces capitatus) is a ubiquitous fungus found in soil, water, air, plants and dairy products. It colonizes the skin, and bronchial and intestinal tract of healthy people producing serious opportunistic infections in patients with haematological malignancies, especially in those with acute leukaemia. Since 1960s its presence is being increasingly recognized in this group of patients. The clinical spectrum of S. capitata disseminated infections is very similar to that produced by Candida, being easily misinterpreted. The associated high mortality and low susceptibility to fluconazole and echinocandins of S. capitata require the acknowledgement of this emergent infection so that it can be properly treated. Case report. We report 5 new cases of S. capitata disseminated infection in patients with advanced haematological malignancies observed in the haematology unit between the years 2004 and 2010, and review the state-of-the-art for diagnosis and treatment of this infection. Conclusions. Based on our experience, the prophylactic use of or the empirical antifungal treatment with fluconazole and/or echinocandins would not be adequate for oncohaematological patients in those hospitals where S. capitata infection may be highly prevalent (AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Geotrichum , Geotrichum/isolamento & purificação , Patologia Molecular/instrumentação , Patologia Molecular/métodos , Patologia Molecular/normas , Neoplasias Hematológicas/complicações , Neoplasias Hematológicas/diagnóstico , Neoplasias Hematológicas/microbiologia , Anticorpos Antifúngicos , Antifúngicos , Testes de Sensibilidade Microbiana/métodos , Testes de Sensibilidade Microbiana , Sensibilidade e Especificidade , Leucemia Mieloide Aguda/complicações , Leucemia Mieloide Aguda/microbiologia , Azóis/uso terapêuticoRESUMO
Coconut oil is a rich source of beneficial medium chain fatty acids (MCFAs) particularly lauric acid. In this study, the oil was modified into a value-added product using direct modification of substrate through fermentation (DIMOSFER) method. A coconut-based and coconut-oil-added solid-state cultivation using a Malaysian lipolytic Geotrichum candidum was used to convert the coconut oil into MCFAs-rich oil. Chemical characteristics of the modified coconut oils (MCOs) considering total medium chain glyceride esters were compared to those of the normal coconut oil using ELSD-RP-HPLC. Optimum amount of coconut oil hydrolysis was achieved at 29% moisture content and 10.14% oil content after 9 days of incubation, where the quantitative amounts of the modified coconut oil and MCFA were 0.330 mL/g of solid media (76.5% bioconversion) and 0.175 mL/g of solid media (53% of the MCO), respectively. MCOs demonstrated improved antibacterial activity mostly due to the presence of free lauric acid. The highest MCFAs-rich coconut oil revealed as much as 90% and 80% antibacterial activities against Staphylococcus aureus and Escherichia coli, respectively. The results of the study showed that DIMOSFER by a local lipolytic G. candidum can be used to produce MCFAs as natural, effective, and safe antimicrobial agent. The produced MCOs and MCFAs could be further applied in food and pharmaceutical industries.
Assuntos
Fenômenos Fisiológicos Bacterianos/efeitos dos fármacos , Ácidos Graxos/química , Ácidos Graxos/farmacologia , Geotrichum/metabolismo , Óleos de Plantas/metabolismo , Óleos de Plantas/farmacologia , Antibacterianos/química , Antibacterianos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Óleo de Coco , Ácidos Graxos/análise , Peso Molecular , Óleos de Plantas/químicaRESUMO
A new phytotoxic unrearranged ent-pimaradiene diterpene, named chenopodolin, was isolated from the liquid culture of Phoma chenopodicola, a fungal pathogen proposed for the biological control of Chenopodium album, a common worldwide weed of arable crops such as sugar beet and maize. The structure of chenopodolin was established by spectroscopic, X-ray, and chemical methods as (1S,2S,3S,4S,5S,9R,10S,12S,13S)-1,12-acetoxy-2,3-hydroxy-6-oxopimara-7(8),15-dien-18-oic acid 2,18-lactone. At a concentration of 2 mg/mL, the toxin caused necrotic lesions on Mercurialis annua, Cirsium arvense, and Setaria viride. Five derivatives were prepared by chemical modification of chenopodolin functionalities, and some structure-activity relationships are discussed.