RESUMO
Helicobacter pylori, the etiologic agent of chronic-active gastritis and duodenal ulcers in humans, and Helicobacter mustelae, a gastric pathogen in ferrets, bind to phosphatidylethanolamine (PE), a constituent of host gastric mucosal cells, and to gangliotetraosylceramide (Gg4) and gangliotriaosylceramide (Gg3). The effect of a bovine colostrum concentrate (BCC) on the interaction of H. pylori and H. mustelae to their lipid receptors was examined. BCC blocked attachment of both species to Gg4, Gg3, and PE. Partial inhibition of binding was observed with native bovine and human colostra. BCC lacked detectable antibodies (by immunoblotting) to H. pylori surface proteins (adhesins). However, colostral lipid extracts contained PE and lyso-PE that bound H. pylori in vitro. These results indicate that colostrum can block the binding of Helicobacter species to select lipids and that binding inhibition is conferred, in part, by colostral PE or PE derivatives. Colostral lipids may modulate the interaction of H. pylori and other adhesin-expressing pathogens with their target tissues.
Assuntos
Colostro/imunologia , Gangliosídeo G(M2)/análogos & derivados , Glicoesfingolipídeos/metabolismo , Helicobacter pylori/metabolismo , Helicobacter/metabolismo , Fosfatidiletanolaminas/metabolismo , Adesinas Bacterianas/imunologia , Adesinas Bacterianas/metabolismo , Animais , Anticorpos Antibacterianos/análise , Anticorpos Antibacterianos/imunologia , Aderência Bacteriana/imunologia , Bovinos , Cromatografia em Camada Fina , Colostro/química , Feminino , Gangliosídeo G(M2)/imunologia , Gangliosídeo G(M2)/metabolismo , Gangliosídeos , Glicoesfingolipídeos/imunologia , Helicobacter/imunologia , Helicobacter pylori/imunologia , Humanos , Immunoblotting , Metabolismo dos Lipídeos , Lipídeos/análise , Fosfatidiletanolaminas/imunologia , Receptores de Superfície Celular/imunologia , Receptores de Superfície Celular/metabolismoRESUMO
The common mucosal immune system was stimulated by oral immunisation with jack bean urease and the adjuvant cholera toxin. A high level of local antibody and serum antibody was induced in mice following hyperimmunisation with this combination. No cross-reacting antibody was found against either Helicobacter pylori or Helicobacter felis. No protection was observed against oral challenge of immunised mice with living H. felis thus disproving the interesting hypothesis of Pallen and Clayton that plant urease might induce a protective immunity against helicobacter infection.