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1.
Biomed Chromatogr ; 36(8): e5410, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35577531

RESUMO

The aim of this study was to screen out the active ingredients of Yuanhu Zhitong prescription (YZP) before and after vinegar processing to play an anti-alcoholic gastric ulcer role through spectrum-effect relationship. First, the fingerprint of 16 batches of YZP was studied using the ultra-high-performance liquid chromatography-quadrupole mass spectrometry detector analysis (UPLC-QDA) method. Second, gastric lesion was induced by anhydrous ethanol. The degree of gastric mucosa injury was evaluated by hematoxylin and eosin staining, and the contents of malondialdehyde and tumor necrosis factor α and superoxide dismutase were detected using the enzyme-linked immunosorbent assay kit. Sixteen batches of YZP were analyzed using the spectrum-effect relationship method. Finally, absorption, distribution, metabolism, and excretion (ADME) was used to evaluate the bioavailability of potential compounds. The results showed that the UPLC-QDA method could successfully establish the fingerprint of YZP. Hematoxylin and eosin staining and biochemical indicators showed that YZP had obvious anti-alcoholic gastric ulcer action. Coptisine chloride, corydaline, berberine chloride, palmatine, imperatorin, and phellopterin were screened out using the spectrum-effect method, and all of them possessed good bioavailability. The results of this study suggest that YZP plays an anti-ulcer role by exerting antioxidant and anti-inflammatory effects through six main active components.


Assuntos
Medicamentos de Ervas Chinesas , Úlcera Gástrica , Ácido Acético/química , Cromatografia Líquida/métodos , Medicamentos de Ervas Chinesas/química , Amarelo de Eosina-(YS) , Hematoxilina/análise , Humanos , Prescrições , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/tratamento farmacológico
2.
J Histotechnol ; 43(2): 83-89, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31665982

RESUMO

Biological staining of tissue is a crucial procedure in histotechnology. Rudimentary methods for section preparation have often used stains from natural products, although use of synthetic dyes has become the contemporary standard. Artificial dyes increase the operating costs of a laboratory as well as increase the environmental and personnel risks during manufacturing and usage. These considerations have stimulated research to find alternative natural stains from the wide diversity of plant species. The present study investigated the effect of Eucalyptus sp. (Myrtaceae) wood waste extract on histological staining of animal tissues, using a series of pigment concentrations, pH conditions, and temperatures. Eucalyptus wooden slivers were dried, milled, and 1 g, 2 g, and 4 g of the fine powder was subjected to 50% ethanol extraction for 2 days. Staining tests were then performed on formalin fixed paraffin embedded (FFPE) sections. Increasing acetic acid concentrations (1%, 2% and 4%) were added to the extracts and compared to an acid-free extract. Staining was performed at both ambient room temperature (RT) and 60°C. Connective tissue acidophilic components were well-contrasted and a hematoxylin counterstain demonstrated distinct structural differences between matrix and cell nuclei. Therefore, the present findings demonstrate the potential utility of the eucalyptus wood extracts application as a natural stain alternative for routine histology.


Assuntos
Tecido Conjuntivo/patologia , Técnicas Histológicas , Extratos Vegetais/análise , Coloração e Rotulagem , Madeira , Animais , Corantes/análise , Etanol/análise , Eucalyptus/química , Hematoxilina/análise , Madeira/química
3.
Tech Coloproctol ; 21(9): 693-699, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28887714

RESUMO

BACKGROUND: Lymph node metastasis (LNM) is prognostic in colorectal cancer (CRC). However, evaluation by routine haematoxylin and eosin histology (HE) limits nodal examination and is subjective. Missed LNMs from tissue allocation bias (TAB) might under-stage disease, leading to under-treatment. One-step nucleic acid amplification (OSNA) for CK19 messenger ribonucleic acid (mRNA), a marker of LNM, analyses the whole node. The aim of the present systematic review and meta-analysis was to assess recent studies on OSNA versus HE and its implications for CRC staging and treatment. METHODS: Databases including OVID, Medline and Google Scholar were searched for OSNA, LNM and CRC. Study results were pooled using a random-effects model. Summary receiver operator curves (SROC) assessed OSNA's performance in detecting LNM when compared to routine HE histology. RESULTS: Five case-control studies analysing 4080 nodes from 622 patients were included. The summary estimates of pooled results for OSNA were sensitivity 0.90 [95% confidence interval (CI) 0.86-0.93], specificity 0.94 (95% CI 0.93-0.95) and diagnostic odds ratio 179.5 (CI 58.35-552.2, p < 0.0001). The SROC curve indicated a maximum joint sensitivity and specificity of 0.88 and area under the curve of 0.94, p < 0.0001. On average, 5.4% HE-negative nodes were upstaged by OSNA. CONCLUSIONS: OSNA is as good as routine HE. It may avoid TAB and offer a more objective and standardised assay of LNM. However, for upstaging, its usefulness as an adjunct to HE or superiority to HE requires further assessment of the benefits, if any, of adjuvant therapy in patients upstaged by OSNA.


Assuntos
Neoplasias Colorretais/diagnóstico , Linfonodos/patologia , Técnicas de Amplificação de Ácido Nucleico/estatística & dados numéricos , Adulto , Idoso , Estudos de Casos e Controles , Neoplasias Colorretais/genética , Neoplasias Colorretais/patologia , Amarelo de Eosina-(YS)/análise , Feminino , Hematoxilina/análise , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Técnicas de Amplificação de Ácido Nucleico/métodos , Razão de Chances , Prognóstico , Curva ROC , Sensibilidade e Especificidade
4.
J Biophotonics ; 7(7): 483-91, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23281280

RESUMO

The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivity to fluorophore concentration, excitation power and spectral similarity. This study investigates the diagnosis of early cervical cancer using FLIM and a neural network extreme learning machine classifier. A concurrently high sensitivity and specificity of 92.8% and 80.2%, respectively, were achieved. The results suggest that the proposed technique can be used to supplement the traditional histopathological examination of early cervical cancer.


Assuntos
Inteligência Artificial , Interpretação de Imagem Assistida por Computador/métodos , Microscopia de Fluorescência/métodos , Reconhecimento Automatizado de Padrão/métodos , Neoplasias do Colo do Útero/patologia , Algoritmos , Meios de Contraste/análise , Amarelo de Eosina-(YS)/análise , Estudos de Avaliação como Assunto , Feminino , Corantes Fluorescentes/análise , Hematoxilina/análise , Humanos , Aumento da Imagem/métodos , Invasividade Neoplásica , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Coloração e Rotulagem/métodos , Neoplasias do Colo do Útero/química
5.
Parasitology ; 138(10): 1285-95, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21756425

RESUMO

Ectoparasitic copepods have been reported in a wide range of aquatic animals, including crustacean shellfish. However, with the exception of the salmon louse, Lepeophtheirus salmonis, our knowledge of such parasites in commercial species is rudimentary. The current study examines the morphology and pathology of the parasitic copepod, Nicothoë astaci (the 'lobster louse') in its host, the European lobster, Homarus gammarus. Lobsters were sampled from waters surrounding Lundy Island (Bristol Channel, UK) and all individuals collected were found to harbour female adult N. astaci in their gills, with a mean of 47·3 parasites/lobster. The majority of N. astaci were found in the basal region of pleurobranch gills. The parasite was found to attach to gill filaments via its oral sucker, maxillae and maxillipeds, and to feed on host haemolymph (blood) through a funnel-like feeding channel. It caused varying degrees of damage to the host gill, including occlusion of gill filaments and disruption to the vascular system in the central axis. Although there was evidence of extensive host response (haemocytic infiltration) to the parasite, it was displaced from the parasite attachment site and thus was observed in the central gill axis below. The region of gill filament immediately underlying the parasite feeding channel was devoid of such activity suggesting that the parasite interferes with the cellular defence and haemostatic mechanisms of the lobster in order to maintain invasion of the host.


Assuntos
Copépodes/fisiologia , Ectoparasitoses/parasitologia , Brânquias/parasitologia , Interações Hospedeiro-Parasita , Nephropidae/parasitologia , Frutos do Mar/parasitologia , Animais , Copépodes/ultraestrutura , Ectoparasitoses/imunologia , Ectoparasitoses/patologia , Amarelo de Eosina-(YS)/análise , Feminino , Brânquias/imunologia , Brânquias/ultraestrutura , Hematoxilina/análise , Hemócitos/citologia , Hemócitos/imunologia , Hemolinfa/citologia , Interações Hospedeiro-Parasita/imunologia , Masculino , Microscopia Confocal , Nephropidae/anatomia & histologia , Nephropidae/imunologia , Reino Unido
6.
Eksp Klin Farmakol ; 74(8): 13-6, 2011.
Artigo em Russo | MEDLINE | ID: mdl-22232908

RESUMO

Experiment carried out on laboratory animals (rats) were aimed at comparative evaluation of the effect of several neuroprotective drugs under the conditions of model brain ischemia-reperfusion. The experimental methods included staining of brain tissue sections by hematoxiline-eosine, Nissl staining, and expression of NOS1, NOS3, TRAIL by imunnohistological means. The intensity of damage in various parts of brain and the nature of apoptosis without neuroprotection and with popular neuroprotectors (cytoflavin, actovegin, mexidol) and a test drug at the stage ofpreclinical trial (AKF-90-7) were evaluated. Characteristic cytotoxic (coagulative pycnomorphic and colliquative necrosis of neurons) and vascular (hemostasia, erythropedesis) changes were revealed. The neuroprotective effectof drugs decreases in the following order: AKF-90-7 > cytoflavin > actovegin > mexidol.


Assuntos
Encéfalo/efeitos dos fármacos , Glicina/análogos & derivados , Hemostasia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/uso terapêutico , Picolinas/uso terapêutico , Traumatismo por Reperfusão/tratamento farmacológico , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Combinação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Amarelo de Eosina-(YS)/análise , Mononucleotídeo de Flavina/administração & dosagem , Mononucleotídeo de Flavina/uso terapêutico , Glicina/administração & dosagem , Glicina/uso terapêutico , Hematoxilina/análise , Heme/administração & dosagem , Heme/análogos & derivados , Heme/uso terapêutico , Imuno-Histoquímica , Inosina Difosfato/administração & dosagem , Inosina Difosfato/uso terapêutico , Masculino , Necrose/prevenção & controle , Neurônios/metabolismo , Neurônios/patologia , Fármacos Neuroprotetores/administração & dosagem , Niacinamida/administração & dosagem , Niacinamida/uso terapêutico , Óxido Nítrico Sintase Tipo I/análise , Óxido Nítrico Sintase Tipo I/biossíntese , Óxido Nítrico Sintase Tipo III/análise , Óxido Nítrico Sintase Tipo III/biossíntese , Picolinas/administração & dosagem , Ratos , Ratos Endogâmicos , Traumatismo por Reperfusão/sangue , Succinatos/administração & dosagem , Succinatos/uso terapêutico , Ligante Indutor de Apoptose Relacionado a TNF/análise , Ligante Indutor de Apoptose Relacionado a TNF/biossíntese
7.
Breast Cancer Res Treat ; 105(1): 1-5, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17221160

RESUMO

BACKGROUND: The objective was to determine the additional value of pathologic examination using three-level sectioning and immunocytokeratin (ICK) staining of sentinel lymph node (SN) biopsies in cT1-2N0M0 breast carcinoma patients regarding lymph node staging and eligibility of systemic therapy taking primary tumor characteristics in account. METHODS: SN slides of 277 patients out of a total group of 961 patients known to have tumor-positive SNs detected by three-level sectioning and ICK staining were re-examined. Haematoxylin-eosin (HE) slide level three was scanned for tumor deposits, and when present, extra capsular extension, maximum tumor diameter and number of positive SNs was noted. In addition, slides of the axillary dissection of non-SNs were reviewed, with determination of metastasis size and number of positive non-SNs. Primary tumor characteristics (grade, diameter, estrogen receptor) were recorded. RESULTS: In the single-HE examination, 26 cases SN micrometastasis and 6 macrometastasis were missed, 3 cases of micrometastasis were incorrectly classified as isolated tumor cells, and 9 patients with macrometastasis were misclassified as micrometastasis. In addition, in the tumor-negative single-HE examination, additional axillary lymph node dissection (ALND) revealed 6 cases of non-SN metastasis. Taking primary tumor factors into account for adjuvant systemic therapy, 21 patients would have been denied the choice for systemic therapy if single-HE examination was carried out only. CONCLUSIONS: Single-HE examination of SN may result in a reduction of locoregional and systemic treatment according to treatment guidelines then current in the Netherlands.


Assuntos
Neoplasias da Mama/diagnóstico , Neoplasias da Mama/patologia , Queratinas/química , Biópsia de Linfonodo Sentinela/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma/diagnóstico , Carcinoma/patologia , Amarelo de Eosina-(YS)/análise , Feminino , Hematoxilina/análise , Humanos , Imuno-Histoquímica/métodos , Linfonodos/patologia , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica
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