Assuntos
Anemia Falciforme/tratamento farmacológico , Benzaldeídos/uso terapêutico , Fármacos Hematológicos/uso terapêutico , Oxigênio/sangue , Pirazinas/uso terapêutico , Pirazóis/uso terapêutico , Adulto , Anemia Falciforme/sangue , Anemia Falciforme/complicações , Animais , Benzaldeídos/efeitos adversos , Benzaldeídos/farmacologia , Circulação Cerebrovascular/efeitos dos fármacos , Criança , Ensaios Clínicos como Assunto , Método Duplo-Cego , Avaliação Pré-Clínica de Medicamentos , Eritropoetina/biossíntese , Eritropoetina/sangue , Técnicas de Introdução de Genes , Fármacos Hematológicos/efeitos adversos , Fármacos Hematológicos/farmacologia , Hemoglobina Falciforme/química , Hemoglobina Falciforme/efeitos dos fármacos , Humanos , Hipóxia/sangue , Hipóxia/etiologia , Camundongos , Camundongos Transgênicos , Polimerização/efeitos dos fármacos , Pirazinas/efeitos adversos , Pirazinas/farmacologia , Pirazóis/efeitos adversos , Pirazóis/farmacologia , Reticulócitos/metabolismoRESUMO
Effects of 3,5-dimethoxy-4-hydroxybenzoic acid and 2,3,4-trihydroxyacetophenone were studied on haemoglobin S (Hb S) polymerisation, analgesia and inflammation using Hb S solution, rats and mice. UV spectrophotometric procedure was used to monitor the polymerization of the Hb S. Acetic acid induced writhing in mice and egg albumin induced rat paw edema procedures were used to evaluate analgesic and anti-inflammatory activities of the compounds respectively. The results indicate that both drugs inhibit the process of polymerization significantly, possibly by direct action on the Hb S molecules. The drugs inhibited acetic acid induced pain and decreased egg albumin induced oedema. It is concluded that 3,5-dimethoxy-4-hydroxybenzoic acid and 2,3,4-trihydroxyacetophenone may have some value in the management of sickle cell disease.