A influência do laser de baixa intensidade na osseointegração de implantes de titânio ­ revisão sistemática / The influence of low-level laser therapy (LLLT) on the osseointegration of titanium implants ­ a systematic review

Objetivo: fazer uma revisão da literatura sobre o laser de baixa intensidade (LLLT) e seus efeitos na osseointegração dos implantes de titânio. Material e métodos: uma busca eletrônica foi realizada nos bancos de dados PubMed/Medline e Cochrane Library, entre 1995 e 2015, limitando-se aos artigos em inglês e usando as seguintes palavras-chave: "LLLT", "dental implant" e "osseointegration", combinadas por operadores booleanos. Resultados: das 96 referências analisadas inicialmente, apenas 17 artigos foram incluídos (15 estudos em animais, dois estudos em humanos). Quinze artigos mostraram efeitos positivos da LLLT, enquanto dois foram neutros. Houve grande variabilidade no uso da LLLT, sobretudo no que se refere à dose utilizada, ao número de sessões, à energia de irradiação, e ao comprimento de onda. Conclusão: mais pesquisas sobre este tema são necessárias, utilizando doses padronizadas, assim como comprimento de onda e protocolos de aplicação pré-definidos.

Objective: to perform a systematic review on low-level laser therapy (LLLT) and its effects on the osseointegration of titanium implants. Material and methods: an electronic search was on the PubMed/Medline database and Cochrane Library between 1995 e 2015, restricted to the English literature and using the keywords: "LLLT", "dental implant", and "osseointegration" combined by Boolean operators. Results: from the 96 initially retrieved references, only 17 articles were included (15 animal studies, 2 human studies). Fifteen articles showed positive effects, while only two demonstrated neutral effects of LLLT. A great variability was found on LLLT regarding doses, number of sessions, energy, and wavelengths used. Conclusion: further research on this topic need to be made using standard doses, wavelengths, and pre-defi ned application protocols.

A laser ablation ICP-MS based method for multiplexed immunoblot analysis: applications to manganese-dependent protein dynamics of photosystem II in barley (Hordeum vulgare L.).

Manganese (Mn) constitutes an essential co-factor in the oxygen-evolving complex of photosystem II (PSII). Consequently, Mn deficiency reduces photosynthetic efficiency and leads to changes in PSII composition. In order to study these changes, multiplexed protein assays are advantageous. Here, we developed a multiplexed antibody-based assay and analysed selected PSII subunits in barley (Hordeum vulgare L.). A selection of antibodies were labelled with specific lanthanides and immunoreacted with thylakoids exposed to Mn deficiency after western blotting. Subsequently, western blot membranes were analysed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), which allowed selective and relative quantitative analysis via the different lanthanides. The method was evaluated against established liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) methods, based on data-dependent acquisition (DDA) and selected reaction monitoring (SRM). Manganese deficiency resulted in a general decrease in PSII protein abundances, an effect that was shown to be reversible upon Mn re-supplementation. Specifically, the extrinsic proteins PsbP and PsbQ showed Mn-dependent changes in abundances. Similar trends in the response to Mn deficiency at the protein level were observed when comparing DDA, SRM and LA-ICP-MS results. A biologically important exception to this trend was the loss of PsbO in the SRM analysis, which highlights the necessity of validating protein changes by more than one technique. The developed method enables a higher number of proteins to be multiplexed in comparison to existing immunoassays. Furthermore, multiplexed protein analysis by LA-ICP-MS provides an analytical platform with high throughput appropriate for screening large collections of plants.

Usage of GreenLight HPS 180-W laser vaporisation for treatment of benign prostatic hyperplasia.

INTRODUCTION: Laser therapy has gained increasing acceptance as a relatively less invasive treatment for lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH). From the early procedure of interstitial laser coagulation through to the use of holmium laser enucleation of the prostate, there has been an expanding body of evidence on the efficacy of such procedures. One of the newer lasers is the Green Light HPS 180 W laser. Studies with this GreenLight laser (GLL) (American Medical Systems, Inc, Minnetonka, MN, USA) showing results as good as those of transurethral resection of the prostate (TURP). In this paper, the efficacy of the new GLL 180-W versus the gold standard TURP in patients with LUTS due to BPH was tested in a prospective clinical trial. OBJECTIVE: To compare results of Green light laser (GLL) evaporisation of the prostatae and transurethral resection of the prostate (TURP) for treatment of BPH. MATERIJALS AND METHODS: A total of 62 patients with BPH were randomly assigned to two equal groups: TURP or GLL. RESULTS: Both groups were compared regarding all relevant preoperative, operative, and postoperative parameters. Functional results in terms of improvement of International Prostate Symptom Score (IPSS), maximum flow rate (Qmax), and postvoid residual (PVR) urine were assessed at 1, 3, 6 and 12 mo. A total of 62 patients completed 12 mo of follow-up in the TURP and GLL groups, respectively. Baseline characteristics were comparable. Mean operative time was significantly shorter for TURP. Compared to preoperative values, there was significant reduction in hemoglobin levels at the end of TURP only. A significant difference in favor of GLL was achieved regarding the duration of catheterization and hospital stay. In the GLL, no major intraoperative complications were recorded and none of the patients required blood transfusion. Among TURP patients, 6 required transfusion, 1 developed TUR syndrome, and capsule perforation was observed in 5 patients. There was dramatic improvement in Qmax, IPSS, and GLL compared with preoperative values and the degree of improvement was comparable in both groups at all time points of follow-up. Four TURP patients and one GLL patients developed bladder neck contracture treated by bladder neck incision; none in either group experienced urethral stricture or urinary incontinence. CONCLUSIONS: Compared to transurethral resection of the prostate, GreenLight HPS 180-W laser photoselective vaporization of the prostate is safe and effective in the treatment of patients suffering from lower urinary tract symptoms due to benign prostatic hyperplasia.

Laser em dermatologia: princípios físicos, tipos e indicaçöes / Laser in dermatology: phisycal principles, types and indications

O uso de laser em dermatologia tem se expandido muito nos últimos anos. O avanço tecnológico, em mais de 30 anos, permite que a eficácia e as respostas terapêuticas sejam cada vez melhores. Porém, mais complexos tornam-se a compreensäo do seu uso e suas indicaçöes em dermatologia