RESUMO
The prevalence of reproductive dysfunction in males has risen in the last few years, and alternative therapies are gradually gaining in popularity. Our in vitro study aimed to evaluate the potential impact of Lepidium sativum L. on mice TM3 Leydig cells, concerning basal parameters such as cell viability, cell membrane integrity, and lysosomal activity, after 24 h and 48 h exposure. Moreover, reactive oxygens species generation, sex-steroid hormone secretion, and intercellular communication were quantified. In the present study, the microgreen extract from Lepidium was rich in ferulic acid, 4-OH benzoic acid, and resveratrol, with a significant antioxidant activity. The results showed that lower experimental doses (62.5-250 µg/mL) could positively affect the observed parameters, with significant differences at 250 µg/mL after 24 h and 48 h, respectively. Potential risks could be associated with higher concentrations, starting at 500 µg/mL, 1000 µg/mL, and 2000 µg/mL of Lepidium. Nevertheless, biochemical quantification indicated a significant antioxidant potential and a rich content of biologically active molecules at the applied doses, and time determined the intracellular response of the cultured model.
Assuntos
Lepidium sativum , Lepidium , Animais , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Comunicação Celular , Sobrevivência Celular , Lepidium/metabolismo , Lepidium sativum/química , Células Intersticiais do Testículo/metabolismo , Masculino , Camundongos , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Testosterona/metabolismoRESUMO
Coronopus didymus (L.) Sm. (CD) has been traditionally used to treat pain, rheumatism, and inflammation. This study was planned to appraise the anti-oxidant, anti-inflammatory and anti-arthritic potentials of CD (whole plant) aqueous ethanolic (CDAEE) and aqueous extracts (CDAE) and chemical characterization by high-performance liquid chromatography-diode array detector. In vivo anti-inflammatory (Carrageenan induced paw edema, and Xylene induced ear edema assays) and anti-arthritic potentials were evaluated in Wistar rats. Both extracts showed significant (p < 0.0001) in vitro free radical scavenging and in vitro anti-arthritic potentials by inhibition of protein denaturation and stabilization of the HRBC membrane and anti-oedematogenic potential, whereas more activity was expressed by CDAEE. In complete Freund's adjuvant-induced arthritic model, the CDAEE at 200, 400, and 800 mg kg-1 and methotrexate (1 mg kg-1) profoundly (p < 0.05) reduced the arthritic score and paw edema, restored body and immune organ weight, and altered blood parameters and oxidative stress biomarkers. The qRT-PCR analysis revealed that CDAEE at 400 mg kg-1 significantly (p < 0.0001) downregulated TNF-α (2.22 ± 0.16 fold), IL-6 (2.29 ± 0.05 fold), IL-1ß (2.10 ± 0.01 fold), COX-2 (2.45 ± 0.02 fold), and NF-Ä¸ß (2.72 ± 0.06 fold) and considerably upregulated IL-10 (58.84 ± 0.67%), IL-4 (76.16 ± 2.79%) and I-kß (75.45 ± 0.17%) in arthritic rats in contrast to disease control and methotrexate as evidenced from the joint histology. These findings suggested the antioxidant, anti-inflammatory and anti-arthritic activities of C. didymus, which might be due to the presence of quercetin, ferulic acid, dihydromyricetin, apigenin, vitexin, and kaempferol in CDAEE.
Assuntos
Artrite Experimental , Lepidium , Animais , Anti-Inflamatórios/uso terapêutico , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Cromatografia Líquida de Alta Pressão , Ciclo-Oxigenase 2/metabolismo , Citocinas/metabolismo , Edema/tratamento farmacológico , Lepidium/metabolismo , Metotrexato/efeitos adversos , Estresse Oxidativo , Extratos Vegetais/uso terapêutico , Ratos , Ratos WistarRESUMO
Misuse and abuse of anabolic androgenic steroids (AAS) such as oxymetholone (OM) cause side effects such as male infertility, cardiovascular disorders, musculoskeletal, and hepato-renal dysfunctions in athletes. The aim of this study was to evaluate the protective effects of Lepidium draba L. (L. draba) extract on OM-induced hepato-renal toxicity. Thirty adult male Wistar rats into six groups (n = 5) were randomly divided as follows: control (normal saline), OM (5 mg/kg/day), L. draba-treated (100, 200, and 400 mg/kg/d) plus 5 mg/kg/day OM, and L. draba (400 mg/kg/d) groups. Normal saline, OM and L. draba extract were orally administered for 30 days. On day 31 of the study, hepatic and renal biochemical parameters were measured. Serum cytokines (IL-1ß, IL-10, IL-6) tumor necrosis factor- α (TNF-α) and nitric oxide, levels alongside catalase, glutathione peroxidase, and superoxide dismutase activity were evaluated. Also, changes in liver and kidney histopathology were evaluated. Finally, the anti-oxidant properties of the extract were determined. The results of this study showed that in the groups treated with the L. draba extract, hepatic-renal biochemical parameters improved and also the level of nitric oxide and inflammatory cytokines decreased and the activity of anti-oxidant enzymes increased compared with the OM group. These findings revealed that L. draba, due to its high anti-oxidant properties and high content of polyphenols (especially flavonoids), can improve OM-induced hepato-renal oxidative damages. PRACTICAL APPLICATIONS: L. draba due to its remarkable anti-oxidant and anti-inflammatory properties can protects the kidney and liver injuries against oxymetholone. These features are attributed to the presence of phenolic and flavonoid components. This fidings would be helpful to desgin new therapeutic agents for treating and preventing liver/kidney injuries.
Assuntos
Lepidium , Oximetolona , Animais , Antioxidantes/metabolismo , Citocinas/metabolismo , Lepidium/metabolismo , Masculino , Óxido Nítrico , Estresse Oxidativo , Oximetolona/farmacologia , Extratos Vegetais/farmacologia , Ratos , Ratos Wistar , Solução Salina/farmacologiaRESUMO
The aim of this study was to evaluate the protective effects of Lepidium draba L. extract on cyclophosphamide (CP)-induced oxidative damages to testes in rats using sex hormones, antioxidant properties, inflammatory, and apoptotic pathways. Six groups of male Wistar rats (n = 6/group) received distilled water (NC), CP (100 mg kg-1 day-1 /intraperitoneal), CP with L. draba extract [100 (LDCP 100), 200 (LDCP 200), and 400 (LDCP 400) mg/kg/day/orally] and also only L. draba extract [400 (LD400) mg/kg/day/orally] in 35 days. On day 36 of the study, sperm parameters, serum levels of sex hormones, antioxidant enzyme activity, nitric oxide levels, and inflammatory cytokines and also testicular tissue (ferric reducing antioxidant power and thiobarbituric acid reactive substances levels and expression of ROS-dependent pro/anti-apoptotic pathways) were evaluated. In L. draba-treated groups, especially doses of 200 and 400, in addition to improving sperm parameters and sex hormones (Increased levels of all three hormones luteinizing hormone, follicle-stimulating hormone, and testosterone), serum antioxidant (catalase, superoxide dismutase, and glutathione peroxidase activity increased and nitric oxide levels decreased), and anti-inflammatory properties (levels of IL-6, TNF-α, and IL-1ß decreased and MIF and TGF-ß increased) also showed modification. By strengthening the anti-apoptotic pathway of Keap1/Nrf2/HO1 and inhibiting the apoptotic pathway of Bax/Bcl2/p53/caspase-3, L. draba maintains the structure and function of testicular tissue so that eventually p53-positive testicular cells are reduced and Bcl-2-positive cells increased. L. draba can help to maintain sexual potency and fertility in patients undergoing chemotherapy by controlling their apoptotic, anti-inflammatory, and antioxidant pathways. PRACTICAL APPLICATIONS: Lepidium draba have considerable antioxidant properties and can help to maintain sexual potency and fertility in patients undergoing chemotherapy by controlling their apoptotic, anti-inflammatory, and antioxidant pathways. The present results are useful to find a suitable supplement for improving the sexual performance of patients treated with chemotherapy drugs.
Assuntos
Lepidium , Testículo , Animais , Caspase 3 , Ciclofosfamida/toxicidade , Humanos , Proteína 1 Associada a ECH Semelhante a Kelch , Lepidium/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Folhas de Planta/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2 , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio , Transdução de Sinais , Testículo/metabolismo , Proteína Supressora de Tumor p53 , Proteína X Associada a bcl-2RESUMO
Meyeniines A-C (1-3), three new lignans, two known neolignans (4-5), and three known lignans (6-8) were isolated from the rhizomes of Lepidium meyenii. Their structures were identified by comprehensive spectroscopic analyses and computational methods. Compound 1 represents a unique lignan featuring an aromatic ring migration. Compoundsâ 2 and 4-6 were analyzed by chiral HPLC column as enantiomers. Biological evaluation revealed that compoundâ 8 could inhibit IL-6 production in lipopolysaccharide (LPS) induced RAW264.7 cells in a dose-dependent manner.
Assuntos
Anti-Inflamatórios/química , Lepidium/metabolismo , Lignanas/química , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Interleucina-6/metabolismo , Lignanas/isolamento & purificação , Lignanas/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Conformação Molecular , Extratos Vegetais/química , Células RAW 264.7RESUMO
BACKGROUND: High-temperature stress (HTS) is one of the main environmental stresses that limit plant growth and crop production in agricultural systems. Maca (Lepidium meyenii) is an important high-altitude herbaceous plant adapted to a wide range of environmental stimuli such as cold, strong wind and UV-B exposure. However, it is an extremely HTS-sensitive plant species. Thus far, there is limited information about gene/protein regulation and signaling pathways related to the heat stress responses in maca. In this study, proteome profiles of maca seedlings exposed to HTS for 12 h were investigated using a tandem mass tag (TMT)-based proteomic approach. RESULTS: In total, 6966 proteins were identified, of which 300 showed significant alterations in expression following HTS. Bioinformatics analyses indicated that protein processing in endoplasmic reticulum was the most significantly up-regulated metabolic pathway following HTS. Quantitative RT-PCR (qRT-PCR) analysis showed that the expression levels of 19 genes encoding proteins mapped to this pathway were significantly up-regulated under HTS. These results show that protein processing in the endoplasmic reticulum may play a crucial role in the responses of maca to HTS. CONCLUSIONS: Our proteomic data can be a good resource for functional proteomics of maca and our results may provide useful insights into the molecular response mechanisms underlying herbal plants to HTS.
Assuntos
Lepidium/fisiologia , Proteoma/fisiologia , Clorofila/metabolismo , Regulação da Expressão Gênica de Plantas , Resposta ao Choque Térmico , Lepidium/genética , Lepidium/metabolismo , Redes e Vias Metabólicas , Proteínas de Plantas/metabolismo , Proteínas de Plantas/fisiologia , Proteoma/genética , Proteoma/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Plântula/metabolismo , Plântula/fisiologiaRESUMO
Maca (Lepidium meyenii Walpers) has emerged as a popular functional plant food due to various claimed health effects. This review details the major (i.e., starch, dietary fiber, and protein) and minor constituents (i.e., minerals, non-starch polysaccharides, polyphenols (flavonolignans), macaenes, macamides, glucosinolates, and alkaloids) of maca (root and aerial parts). Diverse health effects of maca are also summarized. Various bioactivities of maca include enhanced reproductive health, antifatigue, antioxidation, neuroprotection, antimicrobial activity, anticancer, hepatoprotection, immunomodulation, and improving skin health and digestive system's function. Plant genetics, botanical parts, processing, extraction, and experimental protocols represent the major factors affecting the chemical composition, physicochemical attributes, and health effects of maca-based products. However, clinical studies to support the claimed health effects of maca and related mechanisms appear to be lacking. Product innovation and diversification in food and non-food utilization of different parts of maca to maximize the value perceptions are suggested.
Assuntos
Lepidium/química , Extratos Vegetais/química , Animais , Antioxidantes/química , Antioxidantes/metabolismo , Humanos , Lepidium/metabolismo , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacologia , Proteínas de Plantas/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Substâncias Protetoras/química , Substâncias Protetoras/metabolismo , Reprodução/efeitos dos fármacosRESUMO
Alpine plants remain the least studied plant communities in terrestrial ecosystems. However, how they adapt to high-altitude environments is far from clear. Here, we used RNA-seq to investigate a typical alpine plant maca (Lepidium meyenii) to understand its high-altitude adaptation at transcriptional and post-transcriptional level. At transcriptional level, we found that maca root significantly up-regulated plant immunity genes in day-time comparing to night-time, and up-regulated abiotic (cold/osmotic) stress response genes in Nov and Dec comparing to Oct. In addition, 17 positively selected genes were identified, which could be involved in mitochondrion. At post-transcriptional level, we found that maca had species-specific characterized alternative splicing (AS) profile which could be influenced by stress environments. For example, the alternative 3' splice site events (A3SS, 39.62%) were predominate AS events in maca, rather than intron retention (IR, 23.17%). Interestingly, besides serine/arginine-rich (SR) proteins and long non-coding RNAs (lncRNAs), a lot of components in nonsense-mediated mRNA decay (NMD) were identified under differential alternative splicing (DAS), supporting AS coupled to NMD as essential mechanisms for maca's stress responses and high-altitude adaptation. Taken together, we first attempted to unveil maca's high-altitude adaptation mechanisms based on transcriptome and post-transcriptome evidence. Our data provided valuable insights to understand the high-altitude adaptation of alpine plants.
Assuntos
Adaptação Biológica/genética , Lepidium/genética , Degradação do RNAm Mediada por Códon sem Sentido , Processamento Alternativo , Altitude , Meio Ambiente , Genes de Plantas , Lepidium/imunologia , Lepidium/metabolismo , Extratos Vegetais/genética , Imunidade Vegetal/genética , Processamento Pós-Transcricional do RNA , Estresse Fisiológico/genética , Transcriptoma/genéticaRESUMO
Lepidium meyenii Walp. (maca) has been utilized in the Andean region because of its edibleness and medicinal value. The aerial parts of maca (APM) were analyzed for protein, total sugar, vitamins, amino acids, and minerals and its characteristic active ingredients at five different growth stages. The results showed the high protein, total sugar, vitamin C, niacin, potassium, and calcium contents of APM. All 17 amino acids and the characteristic active ingredients, namely, macamide, glucosinolates, adenosine, and total saponins, were detected. We examined the effects of maca plant powders on gastric emptying and intestinal propulsion and the levels of serum motilin and gastrin in atropine-treated mice. Benzyl isothiocyanate (BITC) was investigated to identify the potential active material in APM. The results revealed that both maca plant powders and BITC can promote the gastrointestinal prokinetic efficacy. Thus, APM feature potential as new functional vegetable sources.
Assuntos
Trato Gastrointestinal/metabolismo , Lepidium/metabolismo , Componentes Aéreos da Planta/metabolismo , Extratos Vegetais/metabolismo , Verduras/metabolismo , Aminoácidos/análise , Aminoácidos/metabolismo , Animais , Ácido Ascórbico/análise , Ácido Ascórbico/metabolismo , Cálcio/análise , Cálcio/metabolismo , Feminino , Trato Gastrointestinal/química , Glucosinolatos/análise , Glucosinolatos/metabolismo , Lepidium/química , Masculino , Camundongos , Niacina/análise , Niacina/metabolismo , Valor Nutritivo , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Proteínas de Plantas/análise , Proteínas de Plantas/metabolismo , Verduras/químicaRESUMO
KEY MESSAGE: Simultaneous RNAi silencing of the FAD2 and FAE1 genes in the wild species Lepidium campestre improved the oil quality with 80 % oleic acid content compared to 11 % in wildtype. Field cress (Lepidium campestre) is a wild biennial species within the Brassicaceae family with desirable agronomic traits, thus being a good candidate for domestication into a new oilseed and catch crop. However, it has agronomic traits that need to be improved before it can become an economically viable species. One of such traits is the seed oil composition, which is not desirable either for food use or for industrial applications. In this study, we have, through metabolic engineering, altered the seed oil composition in field cress into a premium oil for food processing, industrial, or chemical industrial applications. Through seed-specific RNAi silencing of the field cress fatty acid desaturase 2 (FAD2) and fatty acid elongase 1 (FAE1) genes, we have obtained transgenic lines with an oleic acid content increased from 11 % in the wildtype to over 80 %. Moreover, the oxidatively unstable linolenic acid was decreased from 40.4 to 2.6 %, and the unhealthy erucic acid was reduced from 20.3 to 0.1 %. The high oleic acid trait has been kept stable for three generations. This shows the possibility to use field cress as a platform for genetic engineering of oil compositions tailor-made for its end uses.
Assuntos
Inativação Gênica , Lepidium/metabolismo , Ácido Oleico/metabolismo , Southern Blotting , Segregação de Cromossomos/genética , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Conformação Molecular , Óleos de Plantas/metabolismo , Plantas Geneticamente Modificadas , Interferência de RNA , Reação em Cadeia da Polimerase em Tempo Real , Especificidade da Espécie , Transformação GenéticaRESUMO
BACKGROUND: Macamides with a benzylalkylamide nucleus are characteristic and major bioactive compounds in the functional food maca (Lepidium meyenii Walp). The aim of this study was to explore variations in macamide content among maca from China and Peru. Twenty-seven batches of maca hypocotyls with different phenotypes, sampled from different geographical origins, were extracted and profiled by liquid chromatography with ultraviolet detection/tandem mass spectrometry (LC-UV/MS/MS). RESULTS: Twelve macamides were identified by MS operated in multiple scanning modes. Similarity analysis showed that maca samples differed significantly in their macamide fingerprinting. Partial least squares discriminant analysis (PLS-DA) was used to differentiate samples according to their geographical origin and to identify the most relevant variables in the classification model. The prediction accuracy for raw maca was 91% and five macamides were selected and considered as chemical markers for sample classification. CONCLUSION: When combined with a PLS-DA model, characteristic fingerprinting based on macamides could be recommended for labelling for the authentication of maca from different geographical origins. The results provided potential evidence for the relationships between environmental or other factors and distribution of macamides. © 2016 Society of Chemical Industry.
Assuntos
Produtos Agrícolas/química , Suplementos Nutricionais/análise , Qualidade dos Alimentos , Alimento Funcional/análise , Hipocótilo/química , Lepidium/química , Alcamidas Poli-Insaturadas/análise , Biomarcadores/análise , China , Cromatografia Líquida de Alta Pressão , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/metabolismo , Análise Discriminante , Inspeção de Alimentos/métodos , Ácidos Heptanoicos/análise , Ácidos Heptanoicos/metabolismo , Hipocótilo/crescimento & desenvolvimento , Hipocótilo/metabolismo , Análise dos Mínimos Quadrados , Lepidium/crescimento & desenvolvimento , Lepidium/metabolismo , Ácidos Palmíticos/análise , Ácidos Palmíticos/metabolismo , Peru , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Alcamidas Poli-Insaturadas/metabolismo , Solventes/química , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Ultravioleta , Ácidos Esteáricos/análise , Ácidos Esteáricos/metabolismo , Espectrometria de Massas em TandemRESUMO
Using maca (Lepidium meyenii) liquor residue as the raw material, dietary fiber (DF) was prepared by chemical (MCDF) and enzymatic (MEDF) methods, respectively, of which the physicochemical and functional properties were comparatively studied. High contents of DF were found in MCDF (55.63%) and MEDF (81.10%). Both fibers showed good functional properties, including swelling capacity, water holding capacity, oil holding capacity, glucose adsorption capacity and glucose retardation index. MEDF showed better functional properties, which could be attributed to its higher content of DF, more irregular surface and more abundant monosaccharide composition. The results herein suggest that maca DF prepared by enzymatic method from liquor residue is a good functional ingredient in food products.
Assuntos
Fibras na Dieta/análise , Lepidium/química , Lepidium/metabolismo , Microscopia Eletrônica de Varredura , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Espectroscopia de Infravermelho com Transformada de Fourier , Água/químicaRESUMO
We have isolated and in silico characterized a cold regulated plastocyanin encoding gene from Lepidium latifolium L designated as LlaDRT. Its cDNA sequence (JN214346) consists of a 504 bp ORF, 48 and 205 bp of 5' and 3' UTR regions, respectively encoding a protein of 17.07 KDa and pI 4.95. In silico and phylogenetic analysis of LlaDRT suggested that the protein has features of a typical plastocyanin family member and of a nearest relative of the predominant isoform of Arabidopsis (PETE2) plastocyanin. Validation of stress response of LlaDRT by qPCR under different abiotic stress regulators viz salicylic acid, jasmonic acid, calcium chloride, ethylene and abscisic acid revealed its possible regulation and crosstalk amongst different pathways.
Assuntos
Lepidium/genética , Sequência de Aminoácidos , Clonagem Molecular , Biologia Computacional , Dano ao DNA/genética , Reparo do DNA/genética , DNA Complementar/genética , Regulação da Expressão Gênica de Plantas , Íntrons , Lepidium/metabolismo , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Sementes/química , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genéticaRESUMO
To gain insights on the effects of color type, cultivation history, and growing site on the composition alterations of maca (Lepidium meyenii Walpers) hypocotyls, NMR profiling combined with chemometric analysis was applied to investigate the metabolite variability in different maca accessions. Maca hypocotyls with different colors (yellow, pink, violet, and lead-colored) cultivated at different geographic sites and different areas were examined for differences in metabolite expression. Differentiations of the maca accessions grown under the different cultivation conditions were determined by principle component analyses (PCAs) which were performed on the datasets derived from their ¹H NMR spectra. A total of 16 metabolites were identified by NMR analysis, and the changes in metabolite levels in relation to the color types and growing conditions of maca hypocotyls were evaluated using univariate statistical analysis. In addition, the changes of the correlation pattern among the metabolites identified in the maca accessions planted at the two different sites were examined. The results from both multivariate and univariate analysis indicated that the planting site was the major determining factor with regards to metabolite variations in maca hypocotyls, while the color of maca accession seems to be of minor importance in this respect.
Assuntos
Agricultura , Hipocótilo/química , Lepidium/química , Cor , Meio Ambiente , Hipocótilo/metabolismo , Lepidium/metabolismo , Espectroscopia de Ressonância Magnética , Metabolômica/métodos , Análise Multivariada , Análise de Componente PrincipalRESUMO
Carcass fat composition of cattle fed a forage-based diet might be inferior with maize silage compared to grass-silage based systems. This was quantified using complete diets with concentrate. To test whether supplements may influence carcass fat properties as well, the maize-silage diet was additionally supplemented either with Acacia mearnsii tannins, garlic, maca or lupines, feeds rich in secondary metabolites. The perirenal fat of 6×6 bulls fed these six diets was analysed for fatty acid profile and shelf life. The n-6/ n-3 ratio was always higher than 11 with the maize silage treatments and 2 with grass silage. The supplements did not affect the occurrence of biohydrogenation intermediates, including rumenic acid. Shelf life, being twice as long with maize compared to grass silage, was either unaffected or tended to be impaired, especially with supplementary garlic. Overall, supplementation was not efficient in improving carcass fat properties of maize-silage fed bulls.
Assuntos
Ácidos Graxos/análise , Carne , Poaceae/química , Silagem/análise , Zea mays/química , Tecido Adiposo/química , Animais , Antioxidantes/farmacologia , Bovinos , Alho/metabolismo , Lepidium/metabolismo , Lupinus/metabolismo , Oxirredução , Taninos/farmacologiaRESUMO
Se realizó una recopilación de información técnica y científica relativa a Lepidium meyenii Chacón (Maca), una planta de la familia Brassicaceae que crece en Los Andes Centrales del Perú, entre los 4.000 y 4.500 msnm. Maca ha sido tradicionalmente empleada en la región altoandina debido a supuestas propiedades afrodisíacas y como estimulante de la actividad reproductiva en el hombre y sus animales. Comparativamente con otros vegetales, el análisis químico de la raíz demuestra un alto contenido de carbohidratos, proteínas, lípidos, vitaminas y minerales. Adicionalmente, aminoácidos esenciales y esteroles de alta especificidad que forman parte de neurotransmisores y hormonas, que a su vez cumplen un importante rol funcional en la actividad reproductiva de los mamíferos, se encuentran en alta concentración. Se concluye, a partir de los datos experimentales logrados por variados autores, que el valor nutricional y el efecto sinérgico de los constituyentes naturales de Lepidium meyenii (Maca) deben ser considerados para explicar su acción preventiva de los efectos deletéreos de la altitud sobre la espermatogénesis y estimulante de la fertilidad en sus variados componentes.
Assuntos
Humanos , Animais , Altitude , Infertilidade/tratamento farmacológico , Lepidium/metabolismo , Lepidium/química , Fitoterapia , Plantas Medicinais , Preparações de Plantas/uso terapêutico , Reprodução , Ecossistema AndinoRESUMO
Ionic and structural hetorogeneity of cells, tissues, and organs of plants are associated with a spectrum of electric characteristics such as bioelectric potentials, electrical conductance, and bioelectric permeability. An important determinant for the plant function is electric properties of the cell membranes and organelles which maintain energy and substance exchange with the environment. Enzymes and other biologically active substances have a powerful charge at the molecular level. Finally, all molecules, including those of water, represent dipoles, and this determines their reactive capacity. A major determinant is the bioelectric polarity of a plant is genetically predetermined and cannot be modified. It is an intrinsic structural feature of the organism whose evolution advent was mediated by gravity. An illustrative presentation of polarity is the downward growth of the roots and upward growth of stems in the Earth's gravitation field. However, gravity is a critical, but not the sole determinant of the plant organism polarization. Potent polarizing effects are exerted by light, the electromagnetic field, moisture, and other factors. It is known that plant cultivation in an upturned position is associated with impairment of water and nutrient uptake, resulting in dyscoordination of physiological processes, growth and developmental retardation. These abnormalities were characteristic when early attempts were made to grow plants in weightlessness conditions.