RESUMO
Fungal diseases cause enormous crop losses, but defining the nutrient conditions encountered by the pathogen remains elusive. Here, we generated a mutant strain of the devastating rice pathogen Magnaporthe oryzae impaired for de novo methionine biosynthesis. The resulting methionine-requiring strain grew strongly on synthetic minimal media supplemented with methionine, aspartate or complex mixtures of partially digested proteins, but could not establish disease in rice leaves. Live-cell-imaging showed the mutant could produce normal appressoria and enter host cells but failed to develop, indicating the availability or accessibility of aspartate and methionine is limited in the plant. This is the first report to demonstrate the utility of combining biochemical genetics, plate growth tests and live-cell-imaging to indicate what nutrients might not be readily available to the fungal pathogen in rice host cells.
Assuntos
Magnaporthe/fisiologia , Fenômenos Fisiológicos da Nutrição/fisiologia , Oryza/microbiologia , Ácido Aspártico/metabolismo , Primers do DNA/genética , Funções Verossimilhança , Magnaporthe/genética , Metionina/genética , Metionina/metabolismo , Mutação/genética , Organismos Geneticamente Modificados , FilogeniaRESUMO
In Magnaporthe oryzae, pyriform conidia are the primary inoculum and the main source for disease dissemination in the field. In this study, we identified and characterized the COM1 gene that was disrupted in three insertional mutants producing slender conidia. COM1 encodes a putative transcription regulator unique to filamentous ascomycetes. The com1 disruption and deletion mutants had similar defects in conidium morphology and were significantly reduced in virulence on rice and barley seedlings. Microscopic examination revealed that the Deltacom1 mutants were defective in appressorium turgor generation, penetration, and infectious growth. COM1 was expressed constitutively in M. oryzae. The Com1 protein had putative helix-loop-helix structures and three predicted nuclear localization signal sequences. In transformants expressing COM1(335-613)-enhanced green fluorescent protein fusion constructs, fluorescence signals were observed in the nucleus. Our data indicated that the COM1 gene may encode a novel transcription regulator that regulates conidial development and invasive growth in M. oryzae.
Assuntos
Proteínas Fúngicas/metabolismo , Magnaporthe/metabolismo , Magnaporthe/patogenicidade , Esporos Fúngicos/metabolismo , Esporos Fúngicos/patogenicidade , Virulência/genética , Núcleo Celular/química , Núcleo Celular/genética , Núcleo Celular/metabolismo , Proteínas Fúngicas/genética , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica , Teste de Complementação Genética , Hordeum/microbiologia , Magnaporthe/genética , Magnaporthe/crescimento & desenvolvimento , Dados de Sequência Molecular , Mutação/genética , Cebolas/microbiologia , Oryza/microbiologia , Doenças das Plantas/microbiologia , Esporos Fúngicos/crescimento & desenvolvimentoRESUMO
The plant pathogenic fungus Magnaporthe grisea excretes siderophores of the coprogen-type for iron acquisition and uses ferricrocin for intracellular iron storage. In the present report we characterize mutants with defects in extracellular siderophore biosynthesis. Deletion of the M. grisea SSM2 gene, which encodes a non-ribosomal peptide synthetase, resulted in a loss of the production of all coprogens. The mutant strains had a reduced growth rate, produced fewer conidia and were more sensitive to oxidative stress. Ferricrocin production was not affected. Upon deletion of M. grisea OMO1, a gene predicted to encode an L-ornithine-N(5)-monooxygenase, no siderophores of any type were detected, the strain was aconidial, growth rate was reduced and sensitivity to oxidative stress was increased. Abundance of several proteins was affected in the mutants. The Deltassm2 and Deltaomo1 mutant phenotypes were complemented by supplementation of the medium with siderophores or reintroduction of the respective genes.
Assuntos
Magnaporthe/fisiologia , Estresse Oxidativo , Sideróforos/biossíntese , Esporos Fúngicos/crescimento & desenvolvimento , Estresse Fisiológico , Proteínas Fúngicas/genética , Deleção de Genes , Teste de Complementação Genética , Magnaporthe/efeitos dos fármacos , Magnaporthe/genética , Magnaporthe/crescimento & desenvolvimento , Oxigenases de Função Mista/genética , Peptídeo Sintases/genéticaRESUMO
Rice blast disease caused by Magnaporthe oryzae is the most important fungal disease of rice. To understand the molecular basis of interaction between the fungus and rice, we constructed a cDNA library from a rice-resistant line inoculated with M. oryzae. One hundred and fifty-three cDNA clones were sequence analyzed, of which 129 exhibited significant nucleotide sequence homology to known genes, 21 were homologous to unknown genes, while three clones did not match to any database. However, these three unmatched clones showed sequence homology at protein level in the protein databases and one of them encoded a disease resistance-related protein kinase and was abundant in the EST collection. Northern analysis showed that this disease resistance-related protein kinase gene was induced by inoculation and only expressed in the rice-resistant, but not susceptible, lines. Southern analysis showed that this gene was present in a single copy in the rice genome and co-segregated with the M. oryzae resistance in the cross of the resistant and susceptible lines. This study illustrates that sequencing of ESTs from inoculated resistant plants can reveal genes responsive to pathogen infection, which could help understand plant defense mechanisms.
Assuntos
Regulação Fúngica da Expressão Gênica , Magnaporthe/genética , Magnaporthe/metabolismo , Oryza/microbiologia , Northern Blotting , Southern Blotting , Cálcio/metabolismo , DNA Complementar/metabolismo , Bases de Dados Genéticas , Etiquetas de Sequências Expressas , Biblioteca Gênica , Predisposição Genética para Doença , Imunidade Inata , Doenças das Plantas/genética , Doenças das Plantas/microbiologiaRESUMO
Isolates of Pyricularia grisea from wheat (Triticum aestivum Lam.) and triticale (x Triticosecale Wittmack) spikes with blast symptoms were analyzed by classical (VCG) and molecular (RAPD) techniques. P. grisea mutants, unable to use sodium nitrate (nit) as nitrogen source, were obtained with potassium chlorate. For vegetative compatibility (VCG) tests, genetically complementary nit mutant pairs were inoculated in a medium with sodium nitrate as a single nitrogen source. P. grisea isolates were divided into two vegetative compatibility groups and two RAPD groups. Since vegetative compatible strains may mutually exchange genetic and cytoplasmatic material, the contribution of the parasexual cycle in the genetic variability of Brazilian P. grisea isolates is discussed.
Assuntos
DNA Fúngico/genética , Grão Comestível/microbiologia , Magnaporthe/isolamento & purificação , Triticum/microbiologia , Brasil , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Genes Fúngicos , Teste de Complementação Genética , Variação Genética , Magnaporthe/genética , Magnaporthe/metabolismo , Magnaporthe/fisiologia , Nitratos/metabolismo , Nitrogênio/metabolismo , Doenças das Plantas/microbiologia , Técnica de Amplificação ao Acaso de DNA Polimórfico , Recombinação GenéticaRESUMO
The rice blast fungus Magnaporthe grisea infects plants by means of specialized infection structures known as appressoria. Turgor generated in the appressorium provides the invasive force that allows the fungus to breach the leaf cuticle with a narrow-penetration hypha gaining entry to the underlying epidermal cell. Appressorium maturation in M. grisea involves mass transfer of lipid bodies to the developing appressorium, coupled to autophagic cell death in the conidium and rapid lipolysis at the onset of appressorial turgor generation. Here, we report identification of the principal components of lipid metabolism in M. grisea based on genome sequence analysis. We show that deletion of any of the eight putative intracellular triacylglycerol lipase-encoding genes from the fungus is insufficient to prevent plant infection, highlighting the complexity and redundancy associated with appressorial lipolysis. In contrast, we demonstrate that a peroxisomally located multifunctional, fatty acid beta-oxidation enzyme is critical to appressorium physiology, and blocking peroxisomal biogenesis prevents plant infection. Taken together, our results indicate that, although triacylglycerol breakdown in the appressorium involves the concerted action of several lipases, fatty acid metabolism and consequent generation of acetyl CoA are necessary for M. grisea to complete its prepenetration phase of development and enter the host plant.
Assuntos
Ácidos Graxos/metabolismo , Metabolismo dos Lipídeos/fisiologia , Magnaporthe/metabolismo , Peroxissomos/metabolismo , Plantas/microbiologia , Parede Celular/metabolismo , Parede Celular/ultraestrutura , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regulação Fúngica da Expressão Gênica , Genoma Fúngico , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Lipase/genética , Lipase/metabolismo , Magnaporthe/genética , Magnaporthe/crescimento & desenvolvimento , Microscopia Eletrônica , Microscopia de Fluorescência , Mutação , Cebolas/microbiologia , Oryza/microbiologia , Oxirredução , Peroxissomos/enzimologia , Doenças das Plantas/microbiologiaRESUMO
The Woronin body is a peroxisome-derived dense-core vesicle that is specific to several genera of filamentous ascomycetes, where it has been shown to seal septal pores in response to cellular damage. The Hexagonal peroxisome (Hex1) protein was recently identified as a major constituent of the Woronin body and shown to be responsible for self-assembly of the dense core of this organelle. Using a mutation in the Magnaporthe grisea HEX1 ortholog, we define a dual and essential function for Woronin bodies during the pathogenic phase of the rice blast fungus. We show that the Woronin body is initially required for proper development and function of appressoria (infection structures) and subsequently necessary for survival of infectious fungal hyphae during invasive growth and host colonization. Fungal mycelia lacking HEX1 function were unable to survive nitrogen starvation in vitro, suggesting that in planta growth defects are a consequence of the mutant's inability to cope with nutritional stress. Thus, Woronin body function provides the blast fungus with an important defense against the antagonistic and nutrient-limiting environment encountered within the host plant.