RESUMO
BACKGROUND: The study of coffee polysaccharides-degrading enzymes from the coffee berry borer Hypothenemus hampei, has become an important alternative in the identification for enzymatic inhibitors that can be used as an alternative control of this dangerous insect. We report the cloning, expression and biochemical characterization of a mannanase gene that was identified in the midgut of the coffee berry borer and is responsible for the degradation of the most abundant polysaccharide in the coffee bean. METHODS: The amino acid sequence of HhMan was analyzed by multiple sequence alignment comparisons with BLAST (Basic Local Alignment Search Tool) and CLUSTALW. A Pichia pastoris expression system was used to express the recombinant form of the enzyme. The mannanase activity was quantified by the 3,5-dinitrosalicylic (DNS) and the hydrolitic properties were detected by TLC. RESULTS: An endo-1,4-ß-mannanase from the digestive tract of the insect Hypothenemus hampei was cloned and expressed as a recombinant protein in the Pichia pastoris system. This enzyme is 56% identical to the sequence of an endo-ß-mannanase from Bacillus circulans that belongs to the glycosyl hydrolase 5 (GH5) family. The purified recombinant protein (rHhMan) exhibited a single band (35.5 kDa) by SDS-PAGE, and its activity was confirmed by zymography. rHhMan displays optimal activity levels at pH 5.5 and 30°C and can hydrolyze galactomannans of varying mannose:galactose ratios, suggesting that the enzymatic activity is independent of the presence of side chains such as galactose residues. The enzyme cannot hydrolyze manno-oligosaccharides such as mannobiose and mannotriose; however, it can degrade mannotetraose, likely through a transglycosylation reaction. The K(m) and k(cat) values of this enzyme on guar gum were 2.074 mg ml(-1) and 50.87 s(-1), respectively, which is similar to other mannanases. CONCLUSION: This work is the first study of an endo-1,4-ß-mannanase from an insect using this expression system. Due to this enzyme's importance in the digestive processes of the coffee berry borer, this study may enable the design of inhibitors against endo-1,4-ß-mannanase to decrease the economic losses stemming from this insect.
Assuntos
Clonagem Molecular , Café/parasitologia , Proteínas de Insetos/metabolismo , Manosidases/metabolismo , Gorgulhos/enzimologia , Sequência de Aminoácidos , Animais , Cromatografia em Camada Fina , Clonagem Molecular/métodos , Eletroforese em Gel de Poliacrilamida , Frutas , Galactanos/metabolismo , Galactose/análogos & derivados , Interações Hospedeiro-Parasita , Concentração de Íons de Hidrogênio , Hidrólise , Proteínas de Insetos/genética , Proteínas de Insetos/isolamento & purificação , Cinética , Mananas/metabolismo , Manosidases/genética , Manosidases/isolamento & purificação , Peso Molecular , Oligossacarídeos/metabolismo , Pichia/genética , Gomas Vegetais/metabolismo , Proteínas Recombinantes/metabolismo , Análise de Sequência de DNA , Especificidade por Substrato , Gorgulhos/genéticaRESUMO
Microbial mannanases have become biotechnologically important in industry but their application is limited due to high production cost. In presents study, the extraction of mannanase from fermented Palm Kernel Cake (PKC) in the Solid State Fermentation (SSF) was optimized. Local isolate of Aspergillus terreus SUK-1 was grown on PKC in (SSF) using column bioreactor. The optimum condition were achieved after two washes of fermented PKC by adding of 10% glycerol (v/v) soaked for 10 h at the room temperature with solvent to ratio, 1:5 (w/v).
Assuntos
Arecaceae/metabolismo , Aspergillus/enzimologia , Proteínas de Bactérias/isolamento & purificação , Fermentação , Manosidases/isolamento & purificação , Óleos de Plantas/metabolismo , Aspergillus/crescimento & desenvolvimento , Proteínas de Bactérias/biossíntese , Reatores Biológicos , Glicerol/química , Microbiologia Industrial/instrumentação , Manosidases/biossíntese , Óleo de Palmeira , Solventes/química , Temperatura , Fatores de TempoRESUMO
beta-Mannanase and beta-mannosidase from Aspergillus awamori K4 was produced by solid culture with coffee waste and wheat bran. The optimum composition for enzyme production was 40% coffee waste-60% wheat bran. Two enzymes were partially purified. Optimum pH was about 5 for both enzymes, and optimum temperature was around 80 degrees C for beta-mannanase and 60-70 degrees C for beta-mannosidase. These enzymes produced some oligosaccharides from glucomannan and galactomannan by their hydrolyzing and transferring activities. beta-Mannanase hydrolyzed konjak and locust bean gum 39.1% and 15.8%, respectively. Oligosaccharides of various molecular size were released from glucomannan of konjak, but on the addition of cellulase, mannobiose was released selectively. In locust bean gum, tetra-, tri-, and disaccharides (mannobiose) were mainly released by K4 beta-mannanase. Tetra- and trisaccharides were heterooligosaccharides consisting of galactose and mannose residues. K4 beta-mannosidase had a transglycosylation action, transferring mannose residue to alcohols and sugars like fructose.
Assuntos
Aspergillus/enzimologia , Manosidases/biossíntese , Manosidases/isolamento & purificação , Aspergillus/crescimento & desenvolvimento , Biodegradação Ambiental , Café , Fibras na Dieta , Indução Enzimática , Galactanos/metabolismo , Glicosilação , Concentração de Íons de Hidrogênio , Hidrólise , Mananas/metabolismo , Especificidade por Substrato , beta-ManosidaseRESUMO
Information about the specificity of glycosidase enzymes is important since it affects their use for characterization and synthesis of oligosaccharides. Two alpha-mannosidases (EC 3.2.1.24), I and II, were isolated from rice beans (Vigna umbellata). The native molecular weight of both isozymes was estimated to be 329,000, but pIs of form I were 5.03-5.34 and pIs of form II were 5.46-6.20. The two isozymes were characterized in terms of optimal pH and temperature, effects of metal ions, inhibition by swainsonine and 1-deoxymannojirimycin, and kinetic parameters for p-nitrophenyl-alpha-D-mannopyranoside and Man alpha (1-2)Man. Both enzymes were more specific towards Man alpha (1-2)Man in both hydrolysis and synthesis, but their hydrolytic specificities towards Man alpha (1-3)[Man alpha (1-6)]Man were different.
Assuntos
Fabaceae/enzimologia , Isoenzimas/química , Isoenzimas/metabolismo , Manosidases/química , Manosidases/metabolismo , Plantas Medicinais , Cromatografia Líquida de Alta Pressão , Eletroforese em Gel de Poliacrilamida , Inibidores Enzimáticos/farmacologia , Concentração de Íons de Hidrogênio , Hidrólise , Focalização Isoelétrica , Isoenzimas/isolamento & purificação , Cinética , Manosidases/isolamento & purificação , Metais/farmacologia , Peso Molecular , Extratos Vegetais/análise , Proteínas de Plantas/análise , Proteínas de Plantas/isolamento & purificação , Especificidade por Substrato , TemperaturaRESUMO
Seeds of Dolichos lablab var. typicus (Indian lablab beans) contain a glucose/ mannose specific lectin that was affinity purified on Sepharose mannose columns in our laboratory. The unbound fraction from this matrix showed alpha-mannosidase activity. In the present study this has been purified to homogeneity by a combination of ion-exchange, hydrophobic chromatography and gel filtration. Purified alpha-mannosidase had an apparent molecular weight of 195,000 +/- 5,000 with 4.5% carbohydrate. On SDS-PAGE under reducing conditions, the enzyme dissociated into two major bands corresponding to Mr 66,000 and Mr 44,000. An antibody to the well studied jack bean alpha-mannosidase cross-reacts with the enzyme from the lablab beans suggesting antigenic similarity between these two legume mannosidases.
Assuntos
Fabaceae/enzimologia , Manosidases/isolamento & purificação , Proteínas de Plantas/isolamento & purificação , Plantas Medicinais , Western Blotting , Manosidases/metabolismo , Proteínas de Plantas/metabolismo , alfa-ManosidaseRESUMO
Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.
Assuntos
Lisossomos/enzimologia , Manosidases/química , Sequência de Aminoácidos , DNA Complementar/genética , Estabilidade Enzimática , Humanos , Leucemia/enzimologia , Manosidases/efeitos dos fármacos , Manosidases/genética , Manosidases/isolamento & purificação , Dados de Sequência Molecular , Análise de Sequência , Sulfatos/farmacologia , Células Tumorais Cultivadas , Compostos de Zinco/farmacologia , Sulfato de Zinco , alfa-ManosidaseRESUMO
Jack bean alpha-mannosidase had a wide acceptor specificity and could transfer mannosyl residues to various acceptors such as D-fructose, L-arabinose, maltose, lactose, and sucrose. The structures of the transferred products of branched cyclodextrins (CDs) (glucosyl-beta CD, maltosyl-alpha CD, and maltosyl-beta CD) were found to be alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->6)-beta CD, alpha-D-mannosyl- (1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1-->6)-alpha CD and alpha-D-mannosyl-(1-->6)-alpha-D-glucosyl-(1-->4)-alpha-D-glucosyl-(1--> 6)- beta CD, respectively. Almond alpha-mannosidase also produced the same transmannosylated products of branched CDs.
Assuntos
Ciclodextrinas/metabolismo , Fabaceae/enzimologia , Manose/metabolismo , Manosidases/metabolismo , Nozes/enzimologia , Plantas Medicinais , Sequência de Carboidratos , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Espectroscopia de Ressonância Magnética , Manosidases/isolamento & purificação , Manosídeos/metabolismo , Metilmanosídeos , Dados de Sequência Molecular , Especificidade por Substrato , alfa-ManosidaseAssuntos
Glicosídeo Hidrolases/isolamento & purificação , Glicosídeo Hidrolases/metabolismo , Oligossacarídeos/química , Oligossacarídeos/metabolismo , Animais , Aspergillus/enzimologia , Configuração de Carboidratos , Sequência de Carboidratos , Cromatografia em Gel/métodos , Cromatografia por Troca Iônica/métodos , Café/enzimologia , Fabaceae/enzimologia , Fígado/enzimologia , Manosidases/isolamento & purificação , Manosidases/metabolismo , Dados de Sequência Molecular , Neuraminidase/isolamento & purificação , Neuraminidase/metabolismo , Vírus da Doença de Newcastle/enzimologia , Plantas Medicinais , Especificidade por Substrato , Suínos , alfa-Galactosidase/isolamento & purificação , alfa-Galactosidase/metabolismo , alfa-Glucosidases/isolamento & purificação , alfa-Glucosidases/metabolismo , alfa-Manosidase , beta-Galactosidase/isolamento & purificação , beta-Galactosidase/metabolismo , beta-N-Acetil-Hexosaminidases/isolamento & purificação , beta-N-Acetil-Hexosaminidases/metabolismoRESUMO
Two arylmannosidases (signified as A and B) were purified to homogeneity from soluble and microsomal fractions of mung bean seedlings. Arylmannosidase A from the microsomes appeared the same on native gels and on SDS gels as soluble arylmannosidase A, the same was true for arylmannosidase B. Sedimentation velocity studies indicated that both enzymes were homogeneous, and that arylmannosidase A had a molecular mass of 237 kd while B had a molecular mass of 243 kd. Arylmannosidase A showed two major protein bands on SDS gels with molecular masses of 60 and 55 kd, and minor bands of 79, 39 and 35 kd. All of these bands were N-linked since they were susceptible to digestion by endoglucosaminidase H. In addition, at least the major bands could be detected by Western blots with antibody raised against the xylose moiety of N-linked plant oligosaccharides, and they could also be labeled in soybean suspension cells with [2-3H]mannose. Arylmannosidase B showed three major bands with molecular masses of 72, 55 and 45 kd, and minor bands of 42 and 39 kd. With the possible exception of the 45 and 42 kd bands, all of these bands are glycoproteins. Arylmannosidases A and B showed somewhat different kinetics in terms of mannose release from high-mannose oligosaccharides, but they were equally susceptible to inhibition by swainsonine and mannostatin A. Polyclonal antibody raised against the arylmannosidase B cross-reacted equally well with arylmannosidase A from mung bean seedlings and with arylmannosidase from soybean cells. However, monoclonal antibody against mung bean arylmannosidase A was much less effective against arylmannosidase B. Antibody was used to examine the biosynthesis and structure of the carbohydrate chains of arylmannosidase in soybean cells grown in [2-3H]mannose. Treatment of the purified enzyme with Endo H released approximately 50% of the radioactivity, and these labeled oligosaccharides were of the high-mannose type, i.e. mostly Man9GlcNAc. The precipitated protein isolated from the Endo H treatment still contained 50% of the radioactivity, and this was present in modified structures that probably contain xylose residues.
Assuntos
Fabaceae/enzimologia , Glycine max/enzimologia , Glicoproteínas/isolamento & purificação , Isoenzimas/isolamento & purificação , Manosidases/isolamento & purificação , Plantas Medicinais , Western Blotting , Cromatografia em Gel , Cromatografia por Troca Iônica , Eletroforese em Gel de Poliacrilamida , Glicopeptídeos/análise , Glicoproteínas/metabolismo , Isoenzimas/metabolismo , Cinética , Manosidases/metabolismo , Peso Molecular , Oligossacarídeos/análiseRESUMO
Mannosidase II was purified from mung bean seedlings to apparent homogeneity by using a combination of techniques including DEAE-cellulose and hydroxyapatite chromatography, gel filtration, lectin affinity chromatography, and preparative gel electrophoresis. The release of radioactive mannose from GlcNAc[3H]Man5GlcNAc was linear with time and protein concentration with the purified protein, did not show any metal ion requirement, and had a pH optimum of 6.0. The purified enzyme showed a single band on SDS gels that migrated with the Mr 125K standard. The enzyme was very active on GlcNAcMan5GlcNAc but had no activity toward Man5GlcNAc, Man9GlcNAc, Glc3Man9GlcNAc, or other high-mannose oligosaccharides. It did show slight activity toward Man3GlcNAc. The first product of the reaction of enzyme with GlcNAcMan5GlcNAc, i.e., GlcNAcMan4GlcNAc, was isolated by gel filtration and subjected to digestion with endoglucosaminidase H to determine which mannose residue had been removed. This GlcNAcMan4GlcNAc was about 60% susceptible to Endo H indicating that the mannosidase II preferred to remove the alpha 1,6-linked mannose first, but 40% of the time removed the alpha 1,3-linked mannose first. The final product of the reaction, GlcNAcMan3GlcNAc, was characterized by gel filtration and various enzymatic digestions. Mannosidase II was very strongly inhibited by swainsonine and less strongly by 1,4-dideoxy-1,4-imino-D-mannitol. It was not inhibited by deoxymannojirimycin.