RESUMO
BACKGROUND: Mercury is a naturally occurring heavy metal that finds wide application in industrial and household settings. It exists in three chemical forms which include elemental (Hg0 ), inorganic mercurous (Hg+) or mercuric (Hg++) salts, and organic compounds. All forms are highly toxic, particularly to the nervous, gastrointestinal, and renal systems. Common circumstances of exposure include recreational substance use, suicide or homicide attempts, occupational hazards, traditional medicines, and endemic food ingestions as witnessed in the public health disasters in Minamata Bay, Japan and in Iraq. Poisoning can result in death or long-term disabilities. Clinical manifestations vary with chemical form, dose, rate, and route of exposure. AIMS AND OBJECTIVES: To summarize the incidence of mercury poisoning encountered at an Indian Poison Center and use three cases to highlight the marked variations observed in clinical manifestations and long-term outcomes among poisoned patients based on differences in chemical forms and routes of exposure to mercury. MATERIALS AND METHODS: A structured retrospective review of the enquiry-database of the Poison Information Center and medical records of patients admitted between August 2019 and August 2021 in a tertiary care referral center was performed. All patients with reported exposure to mercury were identified. We analyzed clinical data and laboratory investigations which included heavy metal (arsenic, mercury, and lead) estimation in whole blood and urine samples. Additionally, selected patients were screened for serum voltage-gated potassium ion channels (VGKC)- contactin-associated protein-like 2 (CASPR2) antibodies. Three cases with a classical presentation were selected for detailed case description. RESULTS: Twenty-two cases were identified between August 2019 and August 2021. Twenty (91%) were acute exposures while two (9%) were chronic. Of these, three representative cases have been discussed in detail. Case 1 is a 3.5-year-old girl who was ought to the emergency department with suspected elemental-mercury ingestion after biting a thermometer. Clinical examination was unremarkable. Chest and abdominal radiography revealed radiodense material in the stomach. Subsequent serial radiographs documented distal intestinal transit of the radiodense material. The child remained asymptomatic. This case exemplifies the largely nontoxic nature of elemental mercury ingestion as it is usually not absorbed from the gastrointestinal tract. Case 2 is a 27-year-old lady who presented with multiple linear nodules over both upper limbs after receiving a red intravenous injection for anemia. Imaging revealed metallic-density deposits in viscera and bones. Nodular biopsy was suggestive of mercury granulomas. A 24-hour urine mercury levels were elevated. She was advised chelation therapy with oral dimercaptosuccinic acid (DMSA). Case 3 is a 22-year-old lady who presented with acrodynia, neuromyotonia, tremulousness, postural giddiness, tachycardia, and hypertension for 2 months, associated with intractable, diffuse burning pain over the buttocks and both lower limbs, 1 month after completing a 3-week course of traditional medications for polycystic ovarian syndrome. A 24-hour urine normetanephrine levels and mercury levels were markedly elevated. Serum anti-VGKC antibodies were present. She was treated with glucocorticoids and oral DMSA with a favorable clinical response. CONCLUSIONS: The clinical manifestations of mercury toxicity are highly variable depending on the source, form, and route of mercury exposure and are related to its toxicokinetics.
Assuntos
Intoxicação por Mercúrio , Mercúrio , Venenos , Criança , Feminino , Humanos , Pré-Escolar , Adulto , Adulto Jovem , Centros de Controle de Intoxicações , Intoxicação por Mercúrio/diagnóstico , Mercúrio/efeitos adversos , Mercúrio/farmacocinética , Succímero/uso terapêutico , Venenos/uso terapêuticoRESUMO
Although Zhusha Anshen Pill (ZSASP) is a commonly used traditional prescription for insomnia, the safety of cinnabar in the formula has always been controversial since its initial application in medical fields. Here, we developed a new prescription, Tieshuang Anshen Prescription (TSASP), by improving ZSASP with Fe2+ instead of Hg2+. Besides, TSASP was further optimized by establishing and testing the HPLC fingerprint and its sedative-hypnotic effect of formulas with different compatibility ratios and performing correlation spectrum analysis. The safety of TSASP was also evaluated by HE staining of liver and kidney. In addition, a validated and robust UHPLC-MS/MS method was established to demonstrate the pharmacokinetic characteristics of berberine, palmatine, jatrorrhizine, ligustilide, catalpol, loganin, liquiritin and liquiritigenin after oral administration of TSASP. Our study originally provides a new non-toxic prescription, TSASP, with better sedative-hypnotic effect in comparison with ZSASP, revealing that Fe2+ could replace Hg2+ to eliminate its toxicity and play a sedative role. Meanwhile, we believe that our pharmacokinetics results may contribute valuable reference to both TSASP's specific mechanism of action and its further clinical efficacy and effectiveness research.
Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Hipnóticos e Sedativos/farmacocinética , Ferro/farmacocinética , Locomoção/efeitos dos fármacos , Mercúrio/farmacocinética , Sono/efeitos dos fármacos , Animais , Animais não Endogâmicos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos/métodos , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/química , Feminino , Hipnóticos e Sedativos/análise , Hipnóticos e Sedativos/química , Ferro/análise , Ferro/química , Locomoção/fisiologia , Masculino , Mercúrio/análise , Mercúrio/química , Compostos de Mercúrio/análise , Compostos de Mercúrio/química , Compostos de Mercúrio/farmacocinética , Camundongos , Ratos , Ratos Wistar , Sono/fisiologiaRESUMO
The effects of foliar Se biofortification (Se+) of the lettuce on the transfer and toxicity of Hg from soil contaminated with HgCl2 (H) and soil collected near the former Hg smelter in Idrija (I), to terrestrial food chain are explored, with Spanish slug as a primary consumer. Foliar application of Se significantly increased Se content in the lettuce, with no detected toxic effects. Mercury exerted toxic effects on plants, decreasing plant biomass, photochemical efficiency of the photosystem II (Fv/Fm) and the total chlorophyll content. Selenium biofortification (Se+ test group) had no effect on Hg bioaccumulation in plants. In slugs, different responses were observed in H and I groups; the I/Se+ subgroup was the most strongly affected by Hg toxicity, exhibiting lower biomass, feeding and growth rate and a higher hepatopancreas/ muscle Hg translocation, pointing to a higher Hg mobility in comparison to H group. Selenium increased Hg bioavailability for slugs, but with opposite physiological responses: alleviating stress in H/Se+ and inducing it in I/Se+ group, indicating different mechanisms of Hg-Se interactions in the food chain under HgCl2 and Idrija soil exposures that can be mainly attributed to different Hg speciation and ligand environment in the soil.
Assuntos
Biofortificação/métodos , Cadeia Alimentar , Gastrópodes/metabolismo , Lactuca/metabolismo , Mercúrio/toxicidade , Selênio/farmacologia , Animais , Bioacumulação , Disponibilidade Biológica , Poluentes Ambientais/farmacocinética , Poluentes Ambientais/toxicidade , Cloreto de Mercúrio/farmacocinética , Cloreto de Mercúrio/toxicidade , Mercúrio/farmacocinética , Solo/químicaRESUMO
With the aim of assessing health risk to shark consumers, cadmium, mercury, and selenium were measured in muscle of Sphyrna lewini from four coastal states (Baja California Sur, Sinaloa, Nayarit, and Colima) in western Mexico. According to length of specimens, three age modes were found: juveniles and neonates (the majority of the individuals), preadults and adults. Average concentrations (µg g-1 dry weight) in all the studied individuals followed the order cadmium (0.06), selenium (0.94), and mercury (1.56). The mean concentrations of cadmium and mercury increased significantly (pâ¯<â¯0.001) with mean length of specimens. Overall, hazard quotient and hazard index values were below one so there is no health risk to consumers. According to molar ratios of Hg and Se in the edible portion (muscle) of sharks, and depending on the areas of collection, individuals from Baja California Sur might not be beneficial to consumers.
Assuntos
Cádmio/análise , Mercúrio/análise , Músculos/química , Selênio/análise , Tubarões/metabolismo , Animais , Cádmio/farmacocinética , Humanos , Mercúrio/farmacocinética , México , Medição de Risco , Alimentos Marinhos/análise , Selênio/farmacocinética , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/farmacocinéticaRESUMO
Selenium and mercury were evaluated in tissues and stomachs of the dolphinfish Coryphaena hippurus from two sites in the Southeastern Gulf of California. Hg levels were consistently low and exhibited the following patterns: muscle (0.14⯱â¯0.01⯵g/g wet weight) followed by kidney (0.12⯱â¯0.02⯵g/g), liver (0.11⯱â¯0.01⯵g/g), and gonads (0.04⯱â¯0.00⯵g/g). The maximum of Se was found in the kidney (5.60⯱â¯0.40⯵g/g) and the minimum in muscle (0.60⯱â¯0.01⯵g/g). All the Se:Hg molar ratios were between 3.7 and 697.1, with the minimum in muscle and the highest in gonads. The results indicate a contrasting behavior biomagnification of Hg and Se; smaller fish (<80â¯cm FL) did not exhibit biomagnification, in contrast with larger fish (>90â¯cm FL: 100% for Hg; 65% for Se). These results appear to be related to different feeding habits and availability of prey.
Assuntos
Conteúdo Gastrointestinal/química , Mercúrio/análise , Perciformes , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Exposição Dietética/efeitos adversos , Feminino , Produtos Pesqueiros/análise , Contaminação de Alimentos/análise , Humanos , Rim/química , Masculino , Mercúrio/farmacocinética , México , Músculos/química , Oceano Pacífico , Medição de Risco , Selênio/farmacocinética , Distribuição Tecidual , Poluentes Químicos da Água/farmacocinéticaRESUMO
In this study, low-thermal technology was used to treat the mercury contaminated farmland soil from a chemical plant in Guizhou Province, China. A series of field planting experiments were also aimed at determining the content of total and methyl-Hg in crop plants after thermal treatment. The results showed that the mercury concentration in soils was reduced about 70% from 255.74 mg/kg to 80.63 mg/kg when treated at 350 °C for 30 min in engineering-scale experiments, and the treated soil retained most of its original soil. Organic-bound and residual mercury in treated soil were reduced by 64.1 and 56.4% by means of a sequential extraction procedure, respectively. The total and methyl-mercury concentrations in crops decreased significantly, and the degree of soil mercury accumulation to crop roots has been reduced significantly. The total Hg concentrations in potato and corn were lower than the mercury tolerance limits for food in China, and the Hg concentration of radish was close to the limit. The technology provides a more sustainable remediation method for treating mercury-contaminated farmland soil in future engineering applications.
Assuntos
Produtos Agrícolas/metabolismo , Recuperação e Remediação Ambiental/métodos , Mercúrio/análise , Mercúrio/farmacocinética , Poluentes do Solo/farmacocinética , Agricultura , China , Produtos Agrícolas/efeitos dos fármacos , Produtos Agrícolas/crescimento & desenvolvimento , Mercúrio/química , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/química , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Raphanus/química , Raphanus/efeitos dos fármacos , Raphanus/metabolismo , Solo/química , Poluentes do Solo/análise , Poluentes do Solo/química , Solanum tuberosum/efeitos dos fármacos , Solanum tuberosum/metabolismo , Zea mays/efeitos dos fármacos , Zea mays/metabolismoRESUMO
With the aim of knowing the distribution of As, Hg, and Se in skipjack (Katsuwonus pelamis, Linnaeus, 1758) and yellowfin tuna (Thunnus albacares, Bonnaterre, 1788) from the Eastern Pacific, elemental concentrations were determined in the muscle and liver; As species were also analyzed in the stomach content. Additionally, health risk for consumers was assessed. For both tunas, levels of As and Se were significantly higher (p < 0.05) in the liver than in the muscle. In K. pelamis, Hg concentrations in the muscle were significantly higher (p < 0.05) than those in the liver. In T. albacares, As, Hg, and Se showed a trend to increase with fish dimensions. Arsenic extractability was better in the muscle than in the liver of both species; in K. pelamis, As species were better extracted than in T. albacares. In both tuna species, the most extractable arsenic was arsenobetaine (AsB) and a minor part was dimethylarsinic acid (DMA). The liver contained mainly AsB with some DMA and arsenocholine (AsC). Hazard indexes (HI) indicated no risk from Hg and Se intake through these tuna species. Considering the individual contribution to the HI, Hg contributed more (80 to 86%) than Se. In the context of health risk, none of the As and Hg values were above the permissible limits; however, two samples of T. albacares (9%) and three samples of K. pelamis (12%) had Se concentrations over the limits. If Hg and Se in the edible portion of tuna are considered under the approach of the HBVSe, tuna consumption is beneficial.
Assuntos
Arsênio/análise , Mercúrio/análise , Selênio/análise , Atum/metabolismo , Animais , Arsênio/farmacocinética , Fígado/química , Mercúrio/farmacocinética , Músculos/química , Oceano Pacífico , Alimentos Marinhos/análise , Selênio/farmacocinética , Especificidade da Espécie , Distribuição TecidualRESUMO
Environmental mercury (Hg) contamination is an urgent global health threat. The complexity of Hg in the environment can hinder accurate determination of ecological and human health risks, particularly within the context of the rapid global changes that are altering many ecological processes, socioeconomic patterns, and other factors like infectious disease incidence, which can affect Hg exposures and health outcomes. However, the success of global Hg-reduction efforts depends on accurate assessments of their effectiveness in reducing health risks. In this paper, we examine the role that key extrinsic and intrinsic drivers play on several aspects of Hg risk to humans and organisms in the environment. We do so within three key domains of ecological and human health risk. First, we examine how extrinsic global change drivers influence pathways of Hg bioaccumulation and biomagnification through food webs. Next, we describe how extrinsic socioeconomic drivers at a global scale, and intrinsic individual-level drivers, influence human Hg exposure. Finally, we address how the adverse health effects of Hg in humans and wildlife are modulated by a range of extrinsic and intrinsic drivers within the context of rapid global change. Incorporating components of these three domains into research and monitoring will facilitate a more holistic understanding of how ecological and societal drivers interact to influence Hg health risks.
Assuntos
Cadeia Alimentar , Mercúrio/toxicidade , Risco , Poluentes Químicos da Água/toxicidade , Animais , Animais Selvagens , Monitoramento Ambiental , Humanos , Mercúrio/farmacocinética , Poluentes Químicos da Água/farmacocinéticaRESUMO
Mercury in foods, in inorganic form [Hg(II)] or as methylmercury (CH3Hg), can have adverse effects. Its elimination from foods is not technologically viable. To reduce human exposure, possible alternatives might be based on reducing its intestinal absorption. This study evaluates the ability of 23 dietary components to reduce the amount of mercury that is absorbed and reaches the bloodstream (bioavailability). We determined their effect on uptake of mercury in Caco-2 cells, a model of intestinal epithelium, exposed to Hg(II) and CH3Hg standards and to swordfish bioaccessible fractions. Cysteine, homocysteine, glutathione, quercetin, albumin and tannic reduce bioavailability of both mercury species. Fe(II), lipoic acid, pectin, epigallocatechin and thiamine are also effective for Hg(II). Some of these strategies also reduce Hg bioavailability in swordfish (glutathione, cysteine, homocysteine). Moreover, extracts and supplements rich in these compounds are also effective. This knowledge may help to define dietary strategies to reduce in vivo mercury bioavailability.
Assuntos
Mucosa Intestinal/efeitos dos fármacos , Mercúrio/farmacocinética , Animais , Disponibilidade Biológica , Transporte Biológico , Células CACO-2 , Cisteína/farmacologia , Suplementos Nutricionais , Glutationa/farmacologia , Homocisteína/farmacologia , Humanos , Absorção Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Compostos de Metilmercúrio/farmacocinética , Perciformes , Quercetina/farmacologia , Alimentos MarinhosRESUMO
Zebrafish behavioral model is a powerful tool for neuroscience research. Behavioral changes in the zebrafish are studied by administering drugs. With the aid of automated and open-source MATLAB program, high-accuracy tracking of zebrafish can be achieved, and the important behavioral parameters can be calculated. Although mercury is accepted as a potent neurotoxin, used as a key element for preparing certain Ayurvedic medicines. In this work, mercury-based inorganic compounds, including HgCl2, HgS, and Ayurvedic medicines (Rasasindura and Kajjali) were administrated in zebrafish, and the effects on various behavioral parameters and cortisol levels were studied. A significant change in the basic locomotor parameters of fish was observed including speed (43% reduction), meander (150% increment), and a number of freeze points (125% increment), during 5-day treatment of HgCl2 along with a 3-fold increase in cortisol level against the control groups. Abnormal behavior was also recorded in color preference test, and novel tank diving behavior of HgCl2-treated groups, which can be attributed to the neurotoxicity induced by the HgCl2 administration. Contrary to this, the Rasasindura-treated group showed a significant increase in speed by 33%, decrease in meander by 20%, decrease in freeze points by 30%, and insignificant alteration in cortisol levels, which can be related to the rejuvenating nature of the Ayurvedic medicine Rasasindura. Additionally, Kajjali treated group did not show any substantial changes in zebrafish cortisol level and behavioral parameters except one in the diving test that indicates lowering stress. Similarly, HgS group showed normal behaviors except two irregular motor behaviors identical with the HgCl2 group. From these results, it can be concluded that the mercury-based Ayurvedic Rasasindura and Kajjali did not show any adverse effect or toxicity on zebrafish behavior model.
Assuntos
Comportamento Animal/efeitos dos fármacos , Ayurveda , Compostos de Mercúrio/toxicidade , Mercúrio/toxicidade , Atividade Motora/efeitos dos fármacos , Síndromes Neurotóxicas/fisiopatologia , Peixe-Zebra , Animais , Disponibilidade Biológica , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Feminino , Hidrocortisona/análise , Masculino , Mercúrio/farmacocinética , Compostos de Mercúrio/farmacocinética , Síndromes Neurotóxicas/etiologia , Síndromes Neurotóxicas/metabolismoRESUMO
Zuotai, also named as "gTso thal", a known Tibetan medicinal mixture containing insoluble cubic crystal mercuric sulfide (ß-HgS), has been used to treat diseases with long history. The mercury release ratio from Zuotai in gastrointestinal environment is one determinant factor for its bioavailability and biological effect. However, the information is still scarce now. Therefore, the study was designed to investigate the effect of sulfhydryl biomolecules [L-cysteine (Cys) and glutathione (GSH)] and pH on mercury dissociation from Zuotai, ß-HgS, and hexagonal crystal mercuric sulfide (α-HgS) in artificial gastrointestinal juices or pure water with a 1:100 solid-liquid ratio. And, the digestion and peristalsis of gastrointestinal tract were simulated in vitro. The results showed the following trend for the mercury release ratio of Zuotai, artificial gastric juice > artificial intestinal juice > pure water, whereas the trend for ß-HgS and α-HgS was as follows, artificial intestinal fluid > artificial gastric fluid > pure water. The mercury release ratios of Zuotai, ß-HgS, and α-HgS significantly increased in artificial intestinal juice containing L-Cys or GSH compared to those without sulfhydryl biomolecules in the juice. However, in contrast to the results observed for ß-HgS and α-HgS, the mercury release ratio of Zuotai was reduced remarkably in pure water and artificial gastric juice with Cys or GSH. And, we found that strong acidic or strong alkaline environments promoted the dissociation of mercury from Zuotai, ß-HgS, and α-HgS. Taken together, current findings may contribute to other studies regarding clinical safety and bioavailability of the traditional drug Zuotai containing ß-HgS.
Assuntos
Cisteína/farmacologia , Liberação Controlada de Fármacos/efeitos dos fármacos , Glutationa/farmacologia , Compostos de Mercúrio/farmacocinética , Mercúrio/farmacocinética , Disponibilidade Biológica , Suco Gástrico/química , Suco Gástrico/metabolismo , Trato Gastrointestinal/metabolismo , Concentração de Íons de Hidrogênio , Medicina Tradicional Tibetana , Mercúrio/química , Mercúrio/metabolismo , Compostos de Mercúrio/química , Compostos de Mercúrio/metabolismo , Água/química , Água/metabolismoRESUMO
Fufang Niuhuang Xiaoyan capsule was a classical compound prescription with the efficacy of heat-clearing, detoxification, sedation and anti-inflammation, with cinnabaris as one of its active ingredients. The study focuses on the pharmacokinetics of mercury in rats after oral administration of cinnabaris and Fufang Niuhuang Xiaoyan capsule, in order to explore the effect of combined traditional Chinese medicines on mercury metabolism. In this study, the method of nitric-perchloric acid digestion system coupled with cold atomic-atomic fluorescence spectroscopy (CV-AFS) was adopted to accurately determine mercury in whole blood of rats. Fufang Niuhueng Xiaoyan capsule had three dose schemes of oral administration, namely equivalent clinical dose, 3 times of equivalent clinical dose and 10 times of equivalent clinical dose; And the doses of oral administration of cinnabaris was calculated according to that of Fufang Niuhuang Xiaoyan capsule. SPF grade healthy SD rats were fasted overnight before the oral administration with cinnabaris suspension (or Fufang Niuhuang Xiaoyan capsule suspension). After oral administration of different doses of cinnabaris, no obvious changes in tmax and MRT were observed, while Cmax/dose, AUC0-48 h/dose and AUC0-∞/dose decreased with the increase in dose, indicating that total mercury absorption in body was declining. As the dose increased, Ke, CL/F decreased, and t1/2 increased, indicating that the elimination slowed down, and mercury metabolism showed non-linear dynamic characteristics within a certain range of dose (22-220 mgâ¢kg⻹). The total mercury metabolism in the whole blood of rats after oral administration with different doses of Fufang Niuhuang Xiaoyan capsule also showed non-linear dynamic characteristics. The results were correlated with the low solubility of cinnabaris in the body. Compared with cinnabaris, Fufang Niuhuang Xiaoyan capsule showed no obvious changes in V/F and MRT, while Ke, CL/F, tmax decreased, and t1/2, Cmax/dose, AUC0-48 h/dose, AUC0-∞/dose increased significantly. The results showed that Fufang Niuhuang Xiaoyan capsule accelerated absorption, slowed down elimination and improved the total absorption of mercury in the whole blood, indicating that Fufang Niuhuang Xiaoyan capsule may contain components for promoting absorption and alleviating elimination of mercury. Fufang Niuhuang Xiaoyan capsule had an impact on the pharmacokinetics of cinnabaris, and long-term administration of cinnabaris (Fufang Niuhuang Xiaoyan capsule) was possible to cause accumulation of mercury in the body. This study could explain changes in efficacy of Fufang Niuhuang Xiaoyan capsule, evaluate the rationality of compound medicines containing toxic elements and provide scientific basis for the rational and safe use of Fufang Niuhuang Xiaoyan capsule.
Assuntos
Produtos Biológicos/administração & dosagem , Medicamentos de Ervas Chinesas/administração & dosagem , Compostos de Mercúrio/administração & dosagem , Mercúrio/farmacocinética , Administração Oral , Animais , Área Sob a Curva , Ratos , Ratos Sprague-DawleyRESUMO
The concentrations of mercury (Hg), selenium (Se) and metallothioneins (MT) were evaluated in fetuses, calves, juveniles and adults of the endangered coastal Franciscana dolphin (Pontoporia blainvillei) from Argentina. Mercury concentrations varied among analyzed tissues (liver, kidney, muscle and brain), with liver showing the higher concentrations in all specimens. An age-dependent accumulation was found in liver, kidney and brain. No significant relationship between Hg and MT concentrations was found for all tissues analyzed. Hepatic Hg molar concentrations were positively correlated with those of Se, indicating a great affinity between these two elements. Furthermore, dark granules of HgSe were observed in Kupffer cells in the liver by electron microscopy, suggesting the role of this macrophage in the detoxification of Hg. A transfer of Hg through placenta was proved. The presence of Hg in brain in all age classes did not show concentrations associated with neurotoxicity.
Assuntos
Golfinhos/metabolismo , Feto/metabolismo , Mercúrio/farmacocinética , Metalotioneína/metabolismo , Selênio/metabolismo , Poluentes Químicos da Água/farmacocinética , Animais , Animais Recém-Nascidos , Argentina , Encéfalo/metabolismo , Golfinhos/embriologia , Golfinhos/crescimento & desenvolvimento , Feminino , Feto/química , Inativação Metabólica , Rim/química , Rim/metabolismo , Fígado/metabolismo , Troca Materno-Fetal , Mercúrio/análise , Músculos/química , Músculos/metabolismo , Placenta/metabolismo , Gravidez , Poluentes Químicos da Água/análiseRESUMO
Concentrations of Hg, Se, Cd, Cu, Zn, Fe, Mn and As, in kidney, liver, muscle and blubber from 7 specimens of Stenella coeruleoalba, stranded along the Israeli Mediterranean coast (IMC) from 2006 to 2011 (2011-series) were determined and compared to previous data on S. coeruleoalba from the IMC (2001-series). No differences were observed in essential and toxic elements concentrations, between the two series, except for hepatic Mn which was higher in the latter. Hg/Se molar ratios in blubber, kidney and liver increased linearly with log Hg concentrations, while muscle was more heterogenic in this respect. Means (±SD) of hepatic Hg concentrations (134±89 and 181±200mgkg(-1), from the 2011 and 2001 series, respectively) were similar to that found in 2007-2009 specimens from Spain, possibly reflecting the relatively high natural background levels of mercury in the Mediterranean Sea.
Assuntos
Monitoramento Ambiental/métodos , Metais Pesados/análise , Stenella/metabolismo , Oligoelementos/análise , Poluentes Químicos da Água/análise , Tecido Adiposo/química , Animais , Feminino , Rim/química , Fígado/química , Masculino , Mar Mediterrâneo , Mercúrio/análise , Mercúrio/farmacocinética , Metais Pesados/farmacocinética , Selênio/análise , Selênio/farmacocinética , Espanha , Oligoelementos/farmacocinética , Poluentes Químicos da Água/farmacocinéticaRESUMO
In the present study, the concentration of trace elements, total mercury (Hg) and selenium (Se) and mercury forms (MeHg, Hginorg and HgSe) in the vulnerable coastal dolphins Pontoporia blainvillei and Sotalia guianensis were appraised and compared, using metallothioneins (MT) and glutathione (GSH) as biomarkers for trace element exposure. The trace element concentrations varied between muscle and liver tissues, with liver of all dolphin specimens showing higher Hg and Se concentrations than those found in muscle. Hg, MeHg and Hginorg molar concentrations showed a clear increase with Se molar concentrations in the liver of both dolphins, and Se concentrations were higher than those of Hg on a molar basis. Se plays a relevant role in the detoxification of MeHg in the hepatic tissue of both dolphins, forming Hg-Se amorphous crystals in liver. In contrast, MT were involved in the detoxification process of Hginorg in liver. GSH levels in P. blainvillei and S. guianensis muscle tissue suggest that these dolphins have different diving capacities. Muscle Hg concentrations were associated to this tripeptide, which protects dolphin cells against Hg stress.
Assuntos
Mergulho/fisiologia , Golfinhos/metabolismo , Glutationa/metabolismo , Mercúrio/análise , Metalotioneína/metabolismo , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Biomarcadores/metabolismo , Brasil , Feminino , Inativação Metabólica , Fígado/metabolismo , Masculino , Mercúrio/farmacocinética , Compostos de Mercúrio/análise , Compostos de Mercúrio/farmacocinética , Compostos de Metilmercúrio/análise , Compostos de Metilmercúrio/farmacocinética , Músculos/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Selênio/farmacocinética , Compostos de Selênio/análise , Compostos de Selênio/farmacocinética , Oligoelementos/análise , Oligoelementos/farmacocinética , Poluentes Químicos da Água/farmacocinéticaRESUMO
We conducted a detailed assessment of the maternal transfer of mercury and selenium to eggs in three bird species (n = 107 parents and n = 339 eggs), and developed predictive equations linking contaminant concentrations in eggs to those in six tissues of the mother (blood, muscle, liver, kidney, breast feathers, and head feathers). Mercury concentrations in eggs were positively correlated with mercury concentrations in each of the mother's internal tissues (R(2) ≥ 0.95), but generally not with feathers. For each species, the proportion of mercury transferred to eggs decreased as mercury concentrations in the mother increased. At the same maternal mercury concentration, the proportion of mercury transferred to eggs differed among species, such that Forster's tern (Sterna forsteri) and black-necked stilt (Himantopus mexicanus) females transferred more methylmercury to their eggs than American avocet (Recurvirostra americana) females. Selenium concentrations in eggs also were correlated with selenium concentrations in the mother's liver (R(2) = 0.87). Furthermore, mercury and selenium concentrations in tern eggs were positively correlated with those in the father (R(2) = 0.84). Incubating male terns had 21% higher mercury concentrations in blood compared to incubating females at the same egg mercury concentration. We provide equations to predict contaminant concentrations in eggs from each of the commonly sampled bird tissues.
Assuntos
Aves/metabolismo , Mercúrio/farmacocinética , Óvulo/metabolismo , Selênio/farmacocinética , Animais , Poluentes Ambientais/farmacocinética , Plumas/metabolismo , Feminino , Fígado/metabolismo , Masculino , Compostos de Metilmercúrio/farmacocinéticaRESUMO
α-HgS is the main component of traditional Chinese medicine cinnabar, while ß-HgS is the main component of Tibetan medicine Zuotai. However, there was no comparative study on the dissolution and absorption in gastrointestinal tract and bioaccumulation in organs of mercury in Cinnabar, Zuotai, α-HgS and ß-HgS. In this study, the dissolution process of the four compounds in the human gastrointestinal tract was simulated to determine the mercury dissolutions and compare the mercury dissolution of different medicines and the dissolution-promoting capacity of different solutions. To explore the absorption and bioaccumulation of cinnabar and Zuotai in organisms, mice were orally administered with clinical equivalent doses cinnabar and Zuotai. Meanwhile, a group of mice was given α-HgS and ß-HgS with the equivalent mercury with cinnabar, while another group was given ß-HgS and HgCl2 with the equivalent mercury with Zuotai. The mercury absorption and bioaccumulation capacities of different medicines in mice and their mercury bioaccumulation in different tissues and organs were compared. The experimental results showed a high mercury dissolutions of Zuotai in artificial gastrointestinal fluid, which was followed by ß-HgS, cinnabar and α-HgS. As for the mercury absorption and bioaccumulation in mice, HgCl2 was the highest, ß-HgS was the next, and a-HgS was slightly higher than cinnabar. The organs with the mercury bioaccumulation from high to low were kidney, liver and brain. This study is close to clinical practices and can provide reference for the clinical safe medication as well as a study model for the safety evaluation on heavy metal-containing medicines by observing the mercury dissolution, absorption, distribution and accumulation of mercury-containing medicines cinnabar and zuotai.
Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Trato Gastrointestinal/metabolismo , Compostos de Mercúrio/farmacocinética , Animais , Encéfalo/metabolismo , Medicamentos de Ervas Chinesas/química , Rim/metabolismo , Fígado/metabolismo , Masculino , Mercúrio/química , Mercúrio/farmacocinética , Compostos de Mercúrio/química , Camundongos , SolubilidadeRESUMO
Mercury and selenium were assessed in the sailfish, Istiophorus platypterus, from the Eastern Pacific. Sixty-seven individuals were sampled, muscle, liver, kidney, gonads and the prey found in the stomach contents were isolated during fishing 2011-2013 tournaments. Hg exhibited the following pattern (µg g(-1) wet weight): liver (0.57 ± 0.07)>muscle (0.56 ± 0.04)>kidney (0.44 ± 0.08)>gonad (0.14 ± 0.01). The maximum concentration of Se was found in kidneys (14.1 ± 1.9 µg g(-1)), and the minimum in muscles (0.67 ± 0.03 µg g(-1)). High Se:Hg ratios were found for muscle (4.1 ± 0.3), kidney (132.4 ± 12.1), liver (54.0 ± 4.4) and gonads (88.2 ± 7.9); Hg:Se molar ratios were several orders of magnitude lower (muscle<0.4 and liver, kidney and gonad<0.03). Sailfish feed mainly on fishes and cephalopods with low Hg levels (<0.13 µg g(-1)), these results indicate biomagnification of Hg and Se. The muscle of I. platypterus should be consumed (according the provisional tolerable weekly intake) by people cautiously so as not to exceed the recommended intake of 215 g per week.
Assuntos
Conteúdo Gastrointestinal/química , Mercúrio/análise , Perciformes/metabolismo , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Ingestão de Alimentos , Monitoramento Ambiental , Cadeia Alimentar , Humanos , Fígado/química , Mercúrio/farmacocinética , Músculos/química , Oceano Pacífico , Alimentos Marinhos/normas , Selênio/farmacocinética , Distribuição Tecidual , Poluentes Químicos da Água/farmacocinéticaRESUMO
Mercury (Hg) and selenium (Se) concentrations were evaluated in a planktivorous fish and four size classes of organisms (FSCO), collected at an oligotrophic bay in the Southeastern Brazilian coast. No significant spatial differences between Hg and Se were found in the FSCO within the five sampling points in the bay. Hg and Se concentrations increased with successive increases in the size class of the analyzed plankton, i.e. approximately 3-and 2-fold, respectively, from microplankton to macroplankton. Hg and Se biomagnified throughout the planktonic food web. The smallest size class of organism, seston, composed of both biotic and abiotic portions, and fish showed the highest Hg concentrations. This indicates that Hg is not biomagnifying in the base of the bay food web. Selenium concentrations in fish were approximately 5.9 times higher than those in seston. Hg and Se concentrations in fish were approximately 3.5 and 14.6 times higher than those found in the plankton, respectively.
Assuntos
Monitoramento Ambiental/métodos , Peixes/metabolismo , Cadeia Alimentar , Mercúrio/análise , Plâncton/química , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Brasil , Mercúrio/farmacocinética , Selênio/farmacocinética , Clima Tropical , Poluentes Químicos da Água/farmacocinéticaRESUMO
Mercury accumulates at high levels in marine mammal tissues. However, its speciation is poorly understood. The main goal of this investigation was to establish the relationships among mercury species and selenium (Se) concentrations in toothed-whale muscles at different mercury levels. The concentrations of total mercury (T-Hg), methylmercury (MeHg), inorganic mercury (I-Hg) and Se were determined in the muscles of four toothed-whale species: bottlenose dolphins (n=31), Risso's dolphins (n=30), striped dolphins (n=29), and short-finned pilot whales (n=30). In each species, the MeHg concentration increased with increasing T-Hg concentration, tending to reach a plateau. In contrast, the proportion of MeHg in T-Hg decreased from 90-100% to 20-40%. The levels of T-Hg and Se showed strong positive correlations. Se/I-Hg molar ratios rapidly decreased with the increase of I-Hg and reached almost 1 in all species. These results suggested that the demethylated MeHg immediately formed Se/I-Hg equimolar complex of mercury selenide (HgSe) in their muscles. In addition, an X-ray absorption fine structure analysis (XAFS) of a bottlenose dolphin muscle confirmed that the dominant chemical form of the Se/I-Hg equimolar complex was HgSe. HgSe was mainly localized in cells near the endomysium using electron probe microanalysis (EPMA). These results suggested that the demethylated MeHg finally deposits within muscle cells of bottlenose dolphin as an inert HgSe.