Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 18 de 18
Filtrar
Mais filtros

Medicinas Complementares
Base de dados
Tipo de documento
Intervalo de ano de publicação
1.
J Vet Diagn Invest ; 32(6): 912-917, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33000702

RESUMO

A 6-y-old, 3.5-kg, spayed female Toy Poodle was presented with left forelimb lameness of 2-d duration. Two months before the initial presentation, radiography showed osteolysis of the medial epicondyle of the left humerus, and the left forelimb was amputated. Grossly, the articular villi of the elbow joint were markedly thickened, and the articular cartilage surfaces of the distal humerus and proximal radius had partial erosion. Histologically, granulomatous arthritis and osteomyelitis characterized by the presence of abundant macrophages containing numerous fungi were observed. ITS and ß-tubulin sequences amplified from the isolate from the specimen were 100% and 99% identical to type strain UTHSC D16-145T of Talaromyces georgiensis, respectively. Canine osteoarthritis caused by T. georgiensis has not been reported previously, to our knowledge.


Assuntos
Artrite/veterinária , Micoses/veterinária , Osteomielite/veterinária , Talaromyces/isolamento & purificação , Animais , Artrite/diagnóstico , Artrite/microbiologia , Doenças do Cão/patologia , Cães , Feminino , Membro Anterior/patologia , Micoses/microbiologia , Micoses/patologia , Osteomielite/microbiologia , Radiografia
2.
Int J Paleopathol ; 31: 7-13, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32866768

RESUMO

OBJECTIVE: Describe pathological features on internal and external aspects of the skull of an ancient grey wolf. MATERIALS: Wolf remains that were found at the southwestern settlement Area A of Gravettian site Pavlov I. METHODS: Visual observation and description; microcomputed tomography; porosity and fragmentation indices for internal and external skull features; histological section of the fourth upper premolar tooth. RESULTS: Dorsally, the sagittal crest revealed bone healing and remodeling. The sagittal lesion differential diagnosis was blunt trauma with or without fracture. Ventrally, otic region pathology included severe proliferation and lysis (osteomyelitis). The pathology was not resolvable among differential (microbial) causes of osteomyelitis, although other potential etiologies were ruled out. CONCLUSIONS: Probable first report of otic region osteomyelitis in an ancient grey wolf. SIGNIFICANCE: The proximity of the wolf remains to human-related findings, and presence of red ochre and shells, suggest human involvement in the burial. LIMITATIONS: This is a single specimen with differential diagnoses that were not resolvable to a single definitive diagnosis. SUGGESTIONS FOR FURTHER RESEARCH: Further investigation of the possible anthropological significance of the burial circumstances.


Assuntos
Sepultamento/história , Crânio , Lobos , Animais , Arqueologia , República Tcheca , História Antiga , Osteomielite/diagnóstico por imagem , Osteomielite/patologia , Osteomielite/veterinária , Paleopatologia , Crânio/diagnóstico por imagem , Crânio/patologia
4.
Sci Rep ; 6: 39174, 2016 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-27982110

RESUMO

Effective treatment of osteomyelitis remains a formidable clinical challenge. The rapid emergence of multidrug-resistant bacteria has renewed interest in developing antimicrobial biomaterials using antiseptic silver ions to treat osteomyelitis. However, inadequate local retention and severe cytotoxic effects have limited the clinical use of ionic silver for bone grafts. We recently developed novel porous nano-hydroxyapatite/polyamide 66 (nHP66)-based nanoscaffold materials containing varied concentrations of silver ions (Ag+) (TA-nHAPA66) and oxidized titanium (TiO2), which was added as a second binary element to enhance antibacterial activity and biocompatibility. In this study, we establish a large cohort of rabbit model of experimental osteomyelitis and investigate the in vivo antimicrobial and therapeutic effects of TA-nHP66 biomaterials and their in vivo silver release kinetics. We find the TA-nHP66 scaffolds exhibit potent antibacterial activities against E. coli and S. aureus, support cell adhesion and cell proliferation of pre-osteoblasts, and stimulate osteogenic regulator/marker expression. Moreover, the TA2-nHP66 scaffold exerts potent antibacterial/anti-inflammation effects in vivo and promotes bone formation at the lesion site of osteomyelitis. We further demonstrate that TA2-nHP66 exhibits excellent biosafety profile without apparent systemic toxicities. Therefore, the TA-nHP66 scaffold biomaterials may be further explored as an effective adjuvant therapy for infected bone defects and/or osteomyelitis debridement.


Assuntos
Anti-Infecciosos/farmacologia , Materiais Biocompatíveis/farmacologia , Durapatita/química , Nanopartículas/química , Nylons/química , Prata/química , Titânio/química , Animais , Anti-Infecciosos/química , Anti-Infecciosos/uso terapêutico , Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Biomarcadores/metabolismo , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Camundongos , Nanopartículas/uso terapêutico , Nanopartículas/toxicidade , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteogênese/efeitos dos fármacos , Osteomielite/tratamento farmacológico , Osteomielite/metabolismo , Osteomielite/patologia , Osteomielite/veterinária , Coelhos , Staphylococcus aureus/efeitos dos fármacos
5.
BMC Vet Res ; 12(1): 140, 2016 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-27417195

RESUMO

BACKGROUND: Locomotor disorders and infections by Escherichia coli represent major concerns to the poultry industry worldwide. Avian pathogenic E. coli (APEC) is associated with extraintestinal infections leading to respiratory or systemic disease known as colibacillosis. The most common lesions seen in cases of colibacillosis are perihepatitis, airsacculitis, pericarditis, peritonitis/salpingitis and arthritis. These diseases are responsible for significant economic losses in the poultry industry worldwide. E. coli has been recently isolated from vertebral osteomyelitis cases in Brazil and there are no data on molecular and phenotypic characteristics of E. coli strains isolated from lesions in the locomotor system of broilers. This raised the question whether specific E. coli strains could be responsible for bone lesions in broilers. The aim of this study was to assess these characteristics of E. coli strains isolated from broilers presenting vertebral osteomyelitis and arthritis in Brazil. RESULTS: Fifteen E. coli strains from bone lesions were submitted to APEC diagnosis and setting of ECOR phylogenic group, O serogroup, flagella type, virulence genes content, genetic patterns by Pulsed Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). In addition, bacterial isolates were further characterized through a lethality test, serum resistance test and antibiotic resistance profile. E. coli strains harbored different genetic pattern as assessed by PFGE, regardless of flock origin and lesion site. The strains belonged to seven sequence types (STs) previously described (ST117, ST101, ST131, ST 371 and ST3107) or newly described in this study (ST5766 and ST5856). ECOR group D (66.7 %) was the most frequently detected. The strains belonged to diverse serogroups (O88, O25, O12, and O45), some of worldwide importance. The antibiotic resistance profile confirmed strains' diversity and revealed a high proportion of multidrug-resistant strains (73 %), mainly to quinolones and beta-lactams, including third generation cephalosporin. The percentage of resistance to tetracycline was moderate (33 %) but always associated with multidrug resistance. CONCLUSIONS: Our results demonstrated that vertebral osteomyelitis and arthritis in broilers can be associated with highly diverse E. coli based on molecular and phenotypic characteristics. There was no specific virulence patterns of the E. coli strains associated with vertebral osteomyelitis or arthritis. Also, E. coli strains were frequently multidrug resistant and belonged to STs commonly shared by APEC and human ExPEC strains.


Assuntos
Artrite/veterinária , Infecções por Escherichia coli/veterinária , Escherichia coli/genética , Variação Genética , Osteomielite/veterinária , Doenças das Aves Domésticas/microbiologia , Animais , Antibacterianos/farmacologia , Artrite/microbiologia , Brasil , Galinhas , Escherichia coli/classificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Osteomielite/microbiologia , Fatores de Virulência/genética
6.
J Avian Med Surg ; 29(3): 216-23, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26378668

RESUMO

A 6-week-old, parent-reared peregrine falcon ( Falco peregrinus ) was presented with spastic hypertonus of its hind limbs of unknown origin and duration. Radiologic examination revealed smooth periosteal reactions ventrally at thoracic vertebrae 5 to 7. Contrast-enhanced computed tomography identified the swelling as inflammation; antibiotic, antimycotic, anti-inflammatory, and analgesic treatments were initiated, and vitamins and minerals were supplemented. Because the bird's condition did not improve after 10 days, it was euthanatized and submitted for postmortem examination. On histopathologic examination, chronic, active osteomyelitis was diagnosed in thoracic vertebrae 5 to 7, and chronic, active arthritis was present in both the right shoulder and left elbow joints. Staphylococcus hyicus was isolated from these 3 locations, as well as from lungs and liver, indicating a chronic septic staphylococcosis. Although infections with Staphylococcus species are occasional causes of vertebral osteomyelitis in juvenile poultry with active growth plates, it is only sporadically reported in raptors and companion birds. This case report is the first description of the clinical features and diagnostic and pathologic findings in a juvenile peregrine falcon with hematogenous osteomyelitis and arthritis associated with septicemia caused by S hyicus.


Assuntos
Artrite Infecciosa/veterinária , Doenças das Aves/microbiologia , Falconiformes , Osteomielite/veterinária , Coluna Vertebral/patologia , Infecções Estafilocócicas/veterinária , Staphylococcus hyicus/isolamento & purificação , Animais , Antibacterianos/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Artrite Infecciosa/tratamento farmacológico , Artrite Infecciosa/microbiologia , Artrite Infecciosa/patologia , Doenças das Aves/patologia , Fluoroquinolonas/uso terapêutico , Masculino , Meloxicam , Osteomielite/tratamento farmacológico , Osteomielite/microbiologia , Osteomielite/patologia , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/patologia , Tiazinas/uso terapêutico , Tiazóis/uso terapêutico
7.
Poult Sci ; 94(1): 25-36, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25577793

RESUMO

Bacteria entering the bloodstream via translocation from the gastrointestinal tract spread hematogenously and can trigger bacterial chondronecrosis with osteomyelitis (BCO) by infecting osteochondrotic microfractures in the epiphyseal-physeal cartilage of the proximal femora and tibiae. In experiment 1, broilers were fed control feed or the same feed containing BacPack 2X, which includes the prebiotic IMW50 (a mannan oligosaccharide beta-glucan yeast cell wall product) plus the probiotic Calsporin (Bacillus subtilis C-3102). Broilers reared on wire flooring consistently developed higher incidences of BCO than hatchmates reared on wood shavings litter (≥24 vs. ≤4%, respectively; P=0.001). Adding BacPack 2X to the feed on d 1 through 56 delayed the age of onset and reduced the cumulative incidence of BCO on wire flooring when compared with broilers fed the control feed (24.0 vs. 40.7%, respectively; P=0.003). In experiment 2, broilers reared on wire flooring received tap water on d 1 through 62 (control group) or therapeutic levels of the potent fluoroquinolone antimicrobial enrofloxacin in the water on d 35 through 54 (enrofloxacin group). During enrofloxacin administration, half as many birds developed BCO in the enrofloxacin group when compared with the control group (8.1 vs. 19.5%, respectively, on d 35 through 54; P=0.001), whereas both groups had similar BCO incidences subsequent to withdrawing enrofloxacin on d 55 through 62 (14.8 vs. 18.2% for the enrofloxacin vs. control groups; P=0.386). Cumulative lameness incidences for d 1 through 62 were higher for the control group than for the enrofloxacin group (39.0 vs. 25.8%, respectively; P=0.003). These results demonstrate that wire flooring imposes a rigorous challenge that leads to high incidences of BCO that can be difficult to suppress, even with therapeutic doses of enrofloxacin. Prophylactically adding BacPack 2X to the feed reduced the incidence of BCO lameness by a proportion similar to that achieved with enrofloxacin, indicating that probiotics potentially can provide effective alternatives to antibiotics for reducing BCO lameness attributable to bacterial translocation and hematogenous distribution.


Assuntos
Antibacterianos/uso terapêutico , Antibioticoprofilaxia/veterinária , Galinhas , Coxeadura Animal/tratamento farmacológico , Osteomielite/veterinária , Doenças das Aves Domésticas/tratamento farmacológico , Animais , Enrofloxacina , Pisos e Cobertura de Pisos , Fluoroquinolonas/uso terapêutico , Abrigo para Animais , Incidência , Coxeadura Animal/epidemiologia , Coxeadura Animal/microbiologia , Masculino , Necrose/tratamento farmacológico , Necrose/epidemiologia , Necrose/microbiologia , Necrose/veterinária , Osteomielite/tratamento farmacológico , Osteomielite/epidemiologia , Osteomielite/microbiologia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/microbiologia , Prebióticos/análise , Probióticos/uso terapêutico
8.
N Z Vet J ; 59(1): 46-9, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21328157

RESUMO

CASE HISTORY: An 8-week-old, 16-kg alpaca cria was presented with a 2-week history of acute onset of left forelimb lameness. CLINICAL FINDINGS: The cria was weight-bearing but lame at the walk in the left forelimb. There was swelling, heat and pain on palpation of the soft tissues, extending from the mid-shaft of the humerus to the elbow. There was no evidence of a traumatic injury. Radiologically, there was evidence of osteomyelitis and a sequestrum involving the distal metaphysis of the humerus. The sequestrum was removed surgically. No bacteria were cultured from samples taken during surgery. After an initial improvement, the cria became acutely lame, and developed an abscess at the surgical site, which was drained. A pure growth of a strictly anaerobic, Gram-negative, non-spore-forming, pleomorphic, filamentous rod was cultured from fluid from the abscess. Further radiographs suggested a second sequestrum was present, and this was removed surgically. Apart from partial dehiscence of the wound, the cria recovered well, and was sound and without complications 18 months after surgery. DIAGNOSIS: Osteomyelitis and formation of sequestra in the distal metaphysic of the left humerus. CLINICAL RELEVANCE: There has only been one case of osteomyelitis and bone sequestration without evidence of trauma in alpaca crias reported in the literature. However, anecdotal reports by veterinary practitioners described similar cases of osteomyelitis and bone sequestration, usually affecting the long bones. Further investigation of osteomyelitis and bone sequestration in crias is needed, to define the incidence, determine the pathogenesis, and develop specific recommendations for prevention and treatment.


Assuntos
Camelídeos Americanos , Membro Anterior/patologia , Osteomielite/veterinária , Animais , Antibacterianos/uso terapêutico , Feminino , Membro Anterior/diagnóstico por imagem , Membro Anterior/cirurgia , Osteomielite/tratamento farmacológico , Osteomielite/cirurgia , Radiografia
9.
Aust Vet J ; 88(7): 283-5, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20579037

RESUMO

A 12-week-old female alpaca cria weighing 10 kg presented with a 2-month history of left hindlimb lameness associated with osteomyelitis and sequestration of the shaft and wing of the left ilium. The affected segment of the ilium was surgically removed. Bacteriological culture revealed a small number of mixed aerobic and anaerobic bacteria. The cria recovered well, was discharged from the hospital 8 days after surgery and was paddock sound without complications 8 months later. Osteomyelitis and sequestration of long bones has been reported in alpacas, but further investigation is necessary to define the prevalence and pathogenesis and to develop specific recommendations for prevention and treatment.


Assuntos
Camelídeos Americanos , Hemipelvectomia/veterinária , Ílio/cirurgia , Osteomielite/veterinária , Animais , Camelídeos Americanos/cirurgia , Feminino , Osteomielite/cirurgia , Resultado do Tratamento
10.
J Vet Med Sci ; 70(11): 1257-9, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19057147

RESUMO

A six-year-old female Labrador retriever dog was suffering from osteomyelitis in her hindlimb. A puncture wound caused by a rotted bamboo stick was presumed as the source of infection. The dog suffered from pre-existing aortic stenosis, but otherwise exhibited no significant abnormality in her systemic conditions excluding claudication of the left hindlimb. The results of cytology and pathological examinations of biopsy samples revealed the diagnosis of mycotic osteomyelitis in this dog. Mycological and DNA tests showed the pathogen as the mushroom Schizophyllum commune. Antibiotic sensitivity testing also revealed susceptibility to itraconazole, which was used to successfully treat the dog. This is a rare case of canine basidiomycosis with S. commune as the etiologic agent.


Assuntos
Antifúngicos/uso terapêutico , Doenças do Cão/microbiologia , Itraconazol/uso terapêutico , Micoses/veterinária , Osteomielite/veterinária , Schizophyllum/patogenicidade , Animais , Reabsorção Óssea/microbiologia , Reabsorção Óssea/veterinária , Primers do DNA , DNA Fúngico/genética , Doenças do Cão/tratamento farmacológico , Doenças do Cão/fisiopatologia , Cães , Feminino , Micoses/tratamento farmacológico , Micoses/fisiopatologia , Osteomielite/tratamento farmacológico , Osteomielite/fisiopatologia , Reação em Cadeia da Polimerase , Schizophyllum/genética , Schizophyllum/isolamento & purificação
11.
J Am Anim Hosp Assoc ; 44(6): 320-6, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18981197

RESUMO

Medical records for six dogs treated with tobramycin-impregnated calcium sulfate beads were reviewed for indications, duration of disease, number of beads implanted, complications, radiographic appearance of the beads, and outcomes. Beads were no longer visible on radiographs made 5 weeks after implantation. Osteomyelitis resolved in five of five dogs with follow-up. The lack of complications and the resolution of clinical signs associated with tobramycin calcium sulfate bead implantation support their clinical application in treating osteomyelitis.


Assuntos
Antibacterianos/uso terapêutico , Doenças do Cão/tratamento farmacológico , Osteomielite/veterinária , Tobramicina/uso terapêutico , Animais , Sulfato de Cálcio/química , Cães , Sistemas de Liberação de Medicamentos/veterinária , Feminino , Masculino , Microesferas , Osteomielite/tratamento farmacológico , Fatores de Tempo , Resultado do Tratamento
12.
Vet Comp Orthop Traumatol ; 19(2): 106-9, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16810353

RESUMO

This in vitro experimental study investigated the feasibility for marbofloxacin, a veterinary fluoroquinolone antibiotic, to retain its antibacterial activity after its inclusion in polymethylmethacrylate (PMMA) cement. The assays were conducted on gelose cultures of various types of bacteria isolated from canine spontaneous osteomyelitis. The efficiency of the antibiotics was assessed by using an antibiogram method. Resistance of marbofloxacin to the temperature observed during PMMA polymerization, antimicrobial effect of galenic, useful concentrations and comparison with gentamicin (reference antibiotic for this use) were evaluated. Marbofloxacin retained its antimicrobial activity after being heated to high temperatures reached during polymerization. The more effective galenic form to incorporate into the PMMA monomer was the marbofloxacin powder and the appropriate concentration was 1/40 degrees . In this experiment, marbofloxacin included in PMMA showed a good antimicrobial activity; however this activity was lower than gentamicin added to PMMA on Gram + and Pseudomonas bacteria.Therefore, it seems useful to incorporate marbofloxacin to PMMA cement to treat, or to prevent, osteomyelitis associated with marbofloxacin sensitive bacteria. Nevertheless, the development of a marbofloxacin-PMMA cement requires further evaluation, especially pharmacological, biomechanical and clinical.


Assuntos
Antibacterianos/uso terapêutico , Cimentos Ósseos/química , Inibidores Enzimáticos/uso terapêutico , Fluoroquinolonas/uso terapêutico , Osteomielite/veterinária , Polimetil Metacrilato/química , Quinolonas/uso terapêutico , Animais , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Estudos de Viabilidade , Fluoroquinolonas/farmacologia , Técnicas In Vitro , Testes de Sensibilidade Microbiana/veterinária , Osteomielite/tratamento farmacológico , Quinolonas/farmacologia , Resultado do Tratamento
13.
Poult Sci ; 81(7): 958-65, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12162356

RESUMO

Supplementation with vitamin D3 was previously shown to protect Escherichia coli challenged birds that underwent two dexamethasone (DEX) treatments at 5 and 12 wk of age in an experimental model of turkey osteomyelitis complex (TOC). The purpose of the present study was to determine the effects of dietary supplementation with 10 microg of 1,25 dihydroxyvitamin D3 (1,25D)/ kg feed or 99 microg of 25-hydroxyvitamin D3 (25D)/kg feed on disease resistance in the same model. Birds were fed the supplemented diets continuously and ad libitum. Seven hundred twenty turkey poults were placed into 24 floor pens in a 3 x 2 x 2 design (three vitamin D treatments, two DEX treatments, two E. coli treatments, with two replicate pens per treatment). At 5 wk of age, half of the birds were treated with DEX, and half of the DEX-treated birds and half of the nontreated birds were challenged with E. coli. All mortalities and lame birds were necropsied. At 9 wk, all of the DEX- or E. coli-treated birds were given another series of DEX injections; 2 wk later 10 birds per pen were necropsied. At 12 wk, survivors of the previous challenges were given a third DEX treatment, and all birds were necropsied 2 wk later. After the first series of DEX injections, mortality was increased in the 25D-supplemented birds that were given the DEX treatment and the E. coli challenge. After the second series of DEX injections, the main effect mean BW was significantly lower in birds given 1,25D as compared to controls and 25D-supplemented birds. Mortality was higher in 1,25D-supplemented birds that were challenged with E. coli at 5 wk and treated with DEX at 9 wk as compared to 25D-supplemented birds. The 1,25D-treated birds that were treated with DEX at 5 and 9 wk and challenged with E. coli at 5 wk had higher mortality and air sacculitis scores as compared to controls and 25D-treated birds. The main effect mean mortality was significantly higher in birds given 1,25D as compared to controls and 25D-treated birds. The percentage of birds with TOC lesions was decreased from 27% to 0 by 25D and 1,25D in the groups given two DEX treatments and E. coli challenge. After the third DEX treatment, BW of 1,25D-suppplemented birds was decreased, and mortality and air sacculitis scores were increased. Bone strength was generally increased by supplementation with 1,25D, whereas 25D supplementation increased bone strength only in birds challenged at 5 wk and treated with DEX at Weeks 9 and 12. In this study, supplementation with vitamin D metabolites decreased TOC incidence in E. coli-challenged birds given two DEX treatments. However, toxic effects were observed in most supplemented DEX-treated birds and may be attributed to an additive effect of DEX treatment, E. coli septicemia, and vitamin D supplementation.


Assuntos
Calcifediol/administração & dosagem , Calcitriol/administração & dosagem , Osteomielite/veterinária , Doenças das Aves Domésticas/prevenção & controle , Perus , Fenômenos Fisiológicos da Nutrição Animal , Animais , Peso Corporal , Dexametasona/administração & dosagem , Suplementos Nutricionais , Infecções por Escherichia coli , Glucocorticoides/administração & dosagem , Masculino , Osteomielite/etiologia , Osteomielite/prevenção & controle , Doenças das Aves Domésticas/etiologia
14.
Poult Sci ; 79(7): 1050-6, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10901209

RESUMO

Turkey osteomyelitis complex (TOC) is defined by the US Food Safety Inspection Service (FSIS) to include normal-appearing processed turkey carcasses that contain lesions including green discoloration of the liver, arthritis/synovitis, soft-tissue abscesses, and osteomyelitis of the proximal tibia. The lesions are associated with many different opportunistic organisms, mainly Staphylococcus aureus and Escherichia coli, suggesting that TOC incidence may be influenced more by deficiencies in the host immune response rather than by the virulence of any one organism. This syndrome is primarily a disease of adolescent male turkeys, and birds with TOC lesions have decreased indices of cell-mediated immunity, leading to the hypothesis that defects in the immune response of individuals within flocks of male turkeys may be responsible for the occurrence of these opportunistic infections. We have developed an experimental model for this disease in which treatment with dexamethasone (DEX), either with or without air sac inoculation with Escherichia coli, produces all of the lesions associated with TOC. These studies suggest that TOC is a result of stress-induced immunosuppression in a subpopulation of male turkeys that respond to the stressors in modern poultry production in a detrimental manner. Supplemental vitamin D3 treatment protected male turkeys from the immunosuppression induced by multiple treatments with DEX and resulted in decreased incidence of mortality, TOC, green liver, and isolation of bacteria from tissues, lower air sacculitis scores, and lower heterophil to lymphocyte ratios than nonsupplemented controls. Vitamin D3 also protected BW; relative weights of the liver, heart, spleen, and bursa; and clinical chemistry values from the effects of DEX treatment. The ability of vitamin D3 supplementation to protect turkeys from the immunosuppressive effects of severe stress emphasizes its role as a prohormone that affects health and disease resistance in turkeys and suggests that variation in the vitamin D receptor genotype may be involved in this disease process. This model has potential value in the identification of other nutritional and physiological immunomodulators that can decrease TOC incidence and will provide a means for the divergent selection of birds more resistant to the stressors of turkey production. In addition, this model will provide justification for management options designed to minimize stress.


Assuntos
Osteomielite/veterinária , Doenças das Aves Domésticas , Perus , Animais , Colecalciferol/administração & dosagem , Dexametasona , Infecções por Escherichia coli/prevenção & controle , Feminino , Glucocorticoides , Terapia de Imunossupressão , Masculino , Osteomielite/microbiologia , Osteomielite/prevenção & controle , Infecções Estafilocócicas/prevenção & controle , Perus/genética , Perus/imunologia
15.
Poult Sci ; 79(5): 672-9, 2000 May.
Artigo em Inglês | MEDLINE | ID: mdl-10824955

RESUMO

Male turkeys immunosuppressed by injection with dexamethasone (DEX) were given supplemental vitamin D3 in their drinking water in two experiments. In Experiment 1, vitamin D3 was supplemented at a dosage of either 2,064 IU/kg (low level) or 4,128 IU/kg (high level) in drinking water provided ad libitum only from Days 1 through 5 after hatch. In Experiment 2, vitamin D3 was provided at the low dosage for the first 5 d after hatch, followed by treatment with the high dosage for 12 h before and 12 h after each stressful event, which included weekly weighings and two DEX treatments. In both experiments, at 5 wk of age half of the birds were given intramuscular injections of 2 mg/kg DEX on 3 alternating d. In Experiment 1, 100 cfu of Escherichia coli was inoculated into the left thoracic airsac at the time of the third DEX injection. All mortalities were examined, and 10 birds per pen were necropsied 2 wk after treatment and examined for lesions of airsacculitis and turkey osteomyelitis complex (TOC). Four birds per pen were bled before necropsy, and white blood cell total counts, differential white blood cell counts, and clinical chemistry values were determined. In Experiment 2, healthy surviving birds were grown for an additional 5-wk period, after which the DEX-treated birds were given a second series of DEX injections and were bled and necropsied 2 wk later. There were no significant effects of vitamin D3 treatment in combined general linear models analysis of Experiment 1; however, when birds not treated with DEX or E. coli were compared with those treated with both DEX and E. coli, supplementation with the low level of vitamin D3 significantly decreased TOC incidence. There were no significant effects of vitamin D3 treatment in birds treated with DEX at 5 wk of age in Experiment 2. However, when surviving birds were given a second DEX treatment at 12 wk, vitamin D3 treatment resulted in significantly lower incidence of mortality, TOC, green liver, isolation of bacteria from tissues, and lower airsacculitis scores and heterophil-to-lymphocyte ratios than controls. Vitamin D3 also improved BW, relative weights of the liver and heart, and serum levels of glucose and alanine aminotransferase (ALT) of birds receiving two treatments with DEX. The ability of vitamin D3 supplementation to protect turkeys from the immunosuppressive effects of multiple DEX treatments emphasizes the role of vitamin D3 as a prohormone that affects health and disease resistance in turkeys.


Assuntos
Colecalciferol/farmacologia , Osteomielite/veterinária , Doenças das Aves Domésticas/prevenção & controle , Estresse Fisiológico/veterinária , Perus , Alanina Transaminase/sangue , Fosfatase Alcalina/sangue , Animais , Aspartato Aminotransferases/sangue , Glicemia/análise , Peso Corporal , Colecalciferol/administração & dosagem , Colecalciferol/uso terapêutico , Creatina Quinase/sangue , Dexametasona/imunologia , Escherichia coli/patogenicidade , Infecções por Escherichia coli/veterinária , Glucocorticoides/imunologia , Análise dos Mínimos Quadrados , Contagem de Leucócitos/veterinária , Modelos Lineares , Fígado/patologia , Contagem de Linfócitos/veterinária , Subpopulações de Linfócitos , Masculino , Osteomielite/metabolismo , Osteomielite/prevenção & controle , Doenças das Aves Domésticas/metabolismo , Distribuição Aleatória , Estresse Fisiológico/complicações , Ácido Úrico/sangue
16.
Vet Rec ; 145(19): 547-50, 1999 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-10609572

RESUMO

Six rescued grey seal (Halichoerus grypus) pups had traumatic injuries to their hind flippers; three had osteomyelitis without fractures, one had a single fracture and associated osteomyelitis, and two had multiple compound fractures and large open wounds. The medical and surgical methods used to treat these lesions are described. A proprietary waterproof covering appeared to be a better method for dressing the wounds than conventional bandaging.


Assuntos
Antibacterianos/uso terapêutico , Anti-Infecciosos/uso terapêutico , Clindamicina/uso terapêutico , Fluoroquinolonas , Fraturas Ósseas/veterinária , Membro Posterior/lesões , Osteomielite/veterinária , Quinolonas/uso terapêutico , Focas Verdadeiras , Animais , Animais Selvagens , Enrofloxacina , Feminino , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/terapia , Membro Posterior/diagnóstico por imagem , Masculino , Osteomielite/diagnóstico por imagem , Osteomielite/tratamento farmacológico , Radiografia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA