RESUMO
Phytocystatins constitute a multigene family that regulates the activity of endogenous and/or exogenous cysteine proteinases. Cereal crops like wheat are continuously threatened by a multitude of pathogens, therefore cystatins offer to play a pivotal role in deciding the plant response. In order to study the need of having diverse specificities and activities of various cystatins, we conducted comparative analysis of six wheat cystatins (WCs) with twelve rice, seven barley, one sorghum and ten corn cystatin sequences employing different bioinformatics tools. The obtained results identified highly conserved signature sequences in all the cystatins considered. Several other motifs were also identified, based on which the sequences could be categorized into groups in congruence with the phylogenetic clustering. Homology modeling of WCs revealed 3D structural topology so well shared by other cystatins. Protein-protein interaction of WCs with papain supported the notion that functional diversity is a con-sequence of existing differences in amino acid residues in highly conserved as well as relatively less conserved motifs. Thus there is a significant conservation at the sequential and structural levels; however, concomitant variations maintain the functional diversity in this protein family, which constantly modulates itself to reciprocate the diversity while counteracting the cysteine proteinases.
Assuntos
Cistatinas , Plantas/metabolismo , Motivos de Aminoácidos/genética , Sequência de Bases , Sequência Conservada/genética , Cistatinas/química , Cistatinas/genética , Cistatinas/metabolismo , Cisteína Proteases , Gorduras Insaturadas na Dieta/metabolismo , Grão Comestível/genética , Grão Comestível/metabolismo , Hordeum/genética , Hordeum/metabolismo , Família Multigênica , Oryza/genética , Oryza/metabolismo , Papaína/genética , Papaína/metabolismo , Óleos de Plantas/metabolismo , Plantas/genética , Poaceae/genética , Poaceae/metabolismo , Estrutura Terciária de Proteína/genética , Proteínas/genética , Proteínas/metabolismo , Pesquisa , Triticum/genética , Triticum/metabolismo , Zea mays/genética , Zea mays/metabolismoRESUMO
Families of papain- and legumain-like cysteine proteinases (CPR) were found in Vicia seeds. cDNAs and antibodies were used to follow organ specificity and the developmental course of CPR-specific mRNAs and polypeptides. Four papain-like cysteine proteinases (CPR1, CPR2, proteinase A and CPR4) from vetch seeds (Vicia sativa L.) were analysed. CPR2 and its mRNA were already found in dry embryonic axes. CPR1 was only detected there during early germination. Both CPR1 and CPR2 strongly increased later during germination. In cotyledons, both CPR1 and CPR2 were only observed one to two days later than in the axis. Proteinase A was not found in axes. In cotyledons it could only be detected several days after seeds had germinated. CPR4 mRNA and polypeptide were already present in embryonic axes and cotyledons during seed maturation and decreased in both organs during germination. Purified CPR1, CPR2 and proteinase A exhibited partially different patterns of globulin degradation products in vitro. Although the cDNA-deduced amino acid sequence of the precursor of proteinase A has an N-terminal signal peptide, the enzyme was not found in vacuoles whereas the other papain-like CPRs showed vacuolar localization. Four different legumain-like cysteine proteinases (VsPB2, proteinase B, VnPB1 and VnPB2) of Vicia species were analysed. Proteinase B and VnPB1 mRNAs were detected in cotyledons and seedling organs after seeds had germinated. Proteinase B degraded globulins isolated from mature vetch seeds in vitro. VsPB2 and proteinase B are localized to protein bodies of maturing seeds and seedlings, respectively, of V. sativa. Like VsPB2 from V sativa, also VnPB2 of V. narbonensis corresponds to vacuolar processing enzymes (betaVPE). Based on these results different functions in molecular maturation and mobilization of storage proteins could be attributed to the various members of the CPR families.
Assuntos
Cotilédone/enzimologia , Cisteína Endopeptidases/metabolismo , Fabaceae/enzimologia , Hipocótilo/enzimologia , Proteínas de Plantas , Plantas Medicinais , Sementes/enzimologia , Fracionamento Químico , Cisteína Endopeptidases/genética , Regulação da Expressão Gênica no Desenvolvimento , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Germinação/genética , Globulinas/metabolismo , Isoenzimas/genética , Isoenzimas/metabolismo , Papaína/genética , Papaína/metabolismo , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sementes/crescimento & desenvolvimento , Vacúolos/enzimologia , Vacúolos/ultraestruturaRESUMO
Expansins are a family of proteins that catalyse long-term extension of isolated plant cell walls due to an as yet unknown biochemical mechanism. They are divided into two groups, the alpha-expansins and beta-expansins, the latter group consisting of grass group I allergens and their vegetative homologs. These grass group I allergens, to which more than 95% of patients allergic to grass pollen possess IgE antibodies, are highly immunologically crossreactive glycoproteins exclusively expressed in pollen of all grasses. Alignments of the amino-acid sequences of grass group I allergens derived from diverse grass species reveal up to 95% homology. It is therefore likely that these molecules share a similar biological function. The major grass group I allergen from timothy grass (Phleum pratense), Phl p 1, was chosen as a model glycoprotein and expressed in the methylotrophic yeast Pichia pastoris to obtain a post-translationally modified and functionally active allergen. The recombinant allergen exhibited proteolytic activity when assayed with various test systems and substrates, which was also subsequently demonstrated with the natural protein, nPhl p 1. These observations are confirmed by amino-acid alignments of Phl p 1 with three functionally important sequence motifs surrounding the active-site amino acids of the C1 (papain-like) family of cysteine proteinases. Moreover, the significantly homologous alpha-expansins mostly share the functionally important C1 sequence motifs. This leads us to propose a C1 cysteine proteinase function for grass group I allergens, which may mediate plant cell wall growth and possibly contributes to the allergenicity of the molecule.
Assuntos
Alérgenos/química , Cisteína Endopeptidases/química , Cisteína Endopeptidases/imunologia , Proteínas de Plantas/química , Proteínas de Plantas/imunologia , Pólen/química , Pólen/imunologia , Alérgenos/genética , Sequência de Aminoácidos , Sequência de Bases , Sequência Consenso , Cisteína Endopeptidases/genética , Primers do DNA/genética , Humanos , Dados de Sequência Molecular , Papaína/química , Papaína/genética , Papaína/imunologia , Pichia/genética , Proteínas de Plantas/genética , Poaceae/química , Poaceae/genética , Poaceae/imunologia , Pólen/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Rinite Alérgica Sazonal/imunologia , Homologia de Sequência de AminoácidosRESUMO
cDNA clones encoding cysteine proteinases from cotyledons of germinated seeds of Vicia sativa L. have been obtained by means of PCR. Degenerate oligonucleotide primers were designed according to conserved amino acid regions of known cysteine proteinases. The deduced amino acid sequences of the cDNA clones encoding VSCYSPR1 and VSCYSPR2 display strong homology to cysteine proteinases of the so called papain superfamily. Northern analyses revealed developmentally regulated expression of both the mRNAs in germinating seeds. The transcripts were shown to be products of two distinct single genes, each exhibiting structural polymorphisms as exposed in few nucleotide substitutions.