RESUMO
Bacterial intercellular communication mediated by small diffusible molecules, known as quorum sensing (QS), is a common mechanism for regulating bacterial colonisation strategies and survival. Influence on QS by plant-derived molecules is proposed as a strategy for combating phytopathogens by modulating their virulence. This work builds upon other studies that have revealed plant-derived QS inhibitors extracted from oak bark (Quercus sp.). It was found that co-incubation of Pectobacterium carotovorum VKM-B-1247 with oak bark extract (OBE) reduced the production of acyl-HSL. This was accompanied by a dose-dependent decrease in the bacterial cellulolytic and protease activity. At the transcriptomic level, the OBE treatment suppressed the main QS-related genes expR/expI. Potato tubers pre-treated with OBE showed resistance to a manifestation of soft-rot symptoms. Analysis of the component composition of the OBE identified several biologically active molecules, such as n-hexadecanoic acid, 2,6-di-tert-butyl-4-methylphenol, butylated hydroxytoluene (BHT), gamma-sitosterol, lupeol, and others. Molecular docking of the binding energy between identified molecules and homology models of LuxR-LuxI type proteins allow to identify potential inhibitors. Collectively, obtained results figure out great potential of widely distributed oak-derived plant material for bacterial control during storage of potato.
Assuntos
Pectobacterium , Quercus , Solanum tuberosum , Proteínas de Bactérias/metabolismo , Simulação de Acoplamento Molecular , Pectobacterium/genética , Pectobacterium/metabolismo , Pectobacterium carotovorum/metabolismo , Casca de Planta/metabolismo , Percepção de Quorum/genética , Solanum tuberosum/microbiologia , Virulência/genéticaRESUMO
Strains of genus Pectobacterium are major cause of soft rot diseases in fruits and vegetables worldwide. Traditional control methods have not been very successful in combating the pathogenesis. As a result there has been an emerging need for developing an alternative ecofriendly and economical strategy. The pathogenesis of Pectobacterium sp. is mediated by quorum sensing (QS) and approaches based on inhibition of QS system to shut down the virulence genes without affecting growth of the pathogen may serve the purpose. Bacillus sp. OA10 associated with purple sponge Haliclona sp. was found to possess extracellular quorum quenching activity. The OA10 extract inhibited QS dependent virulence of Pectobacterium carotovorum subsp. carotovorum BR1 (PccBR1) at low concentrations (0.2 mg) as evident from 77.56 ± 6.17% reduction in potato maceration with complete inhibition by 0.8 mg. Inhibition of plant cell wall degrading enzymes (PCWDE) and carbapenem production by PccBR1 in presence of OA10 extract indicated disruption of the two QS pathways ExpI/ExpR and CarI/CarR in PccBR1. Bacillus sp. OA10 was not found to degrade acyl homoserine lactone (AHL), instead exhibited QSI activity by probably inhibiting AHL synthesis in PccBR1. Absence of enzymatic principle in quorum sensing inhibitor (QSI) is beneficial as enzymes may get inhibited by various factors during their application. OA10 extract did not affect growth of PccBR1, thereby reducing the chance of developing resistance against the QSI. Thus, Bacillus sp. OA10 can prove to be a good prospective candidate for QSI based novel biocontrol formulations.
Assuntos
Bacillus/metabolismo , Pectobacterium carotovorum/metabolismo , Poríferos/microbiologia , Percepção de Quorum/efeitos dos fármacos , Doenças dos Animais/microbiologia , Animais , Antibacterianos/farmacologia , Biodegradação Ambiental , Técnicas de Cocultura , Meios de Cultura/química , Estudos Prospectivos , Solanum tuberosum , Virulência/efeitos dos fármacosRESUMO
BACKGROUND: N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing. We have previously demonstrated the degradation of AHLs by an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through the production of an AHL lactonase. In this study, we cloned the AHL lactonase gene and characterized the purified novel enzyme. RESULTS: Rapid resolution liquid chromatography analysis indicated that purified AidP possesses high AHL-degrading activity on unsubstituted, and 3-oxo substituted homoserine lactones. Liquid chromatography-mass spectrometry analysis confirmed that AidP functions as an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone ring of AHLs. Multiple sequence alignment analysis and phylogenetic analysis suggested that the aidP gene encodes a novel AHL lactonase enzyme. The amino acid composition analysis of aidP and the homologous genes suggested that it might be a cold-adapted enzyme, however, the optimum temperature is 28 °C, even though the thermal stability is low (reduced drastically above 32 °C). Branch-site analysis of several aidP genes of Planococcus sp. branch on the phylogenetic trees also showed evidence of episodic positive selection of the gene in cold environments. Furthermore, we demonstrated the effects of covalent and ionic bonding, showing that Zn2+ is important for activity of AidP in vivo. The pectinolytic inhibition assay confirmed that this enzyme attenuated the pathogenicity of the plant pathogen Pectobacterium carotovorum in Chinese cabbage. CONCLUSION: We demonstrated that AidP is effective in attenuating the pathogenicity of P. carotovorum, a plant pathogen that causes soft-rot disease. This anti-quorum sensing agent is an enzyme with low thermal stability that degrades the bacterial signalling molecules (AHLs) that are produced by many pathogens. Since the enzyme is most active below human body temperature (below 28 °C), and lose its activity drastically above 32 °C, the results of a pectinolytic inhibition assay using Chinese cabbage indicated the potential of this anti-quorum sensing agent to be safely applied in the field trials.
Assuntos
Proteínas de Bactérias/metabolismo , Hidrolases de Éster Carboxílico/metabolismo , Planococcus (Bactéria)/enzimologia , 4-Butirolactona/análogos & derivados , 4-Butirolactona/metabolismo , Sequência de Aminoácidos , Regiões Antárticas , Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Hidrolases de Éster Carboxílico/química , Hidrolases de Éster Carboxílico/isolamento & purificação , Modelos Moleculares , Pectinas/metabolismo , Pectobacterium carotovorum/metabolismo , Percepção de Quorum , Análise de Sequência de Proteína , Especificidade por SubstratoRESUMO
Iron is an important nutrient for the survival and growth of many organisms. In order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. The ferric uptake regulator protein (Fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. However, to date, the role played by Fur in the biology of Pectobacterium carotovorum subsp. brasiliense (Pcb1692), an important pathogen of potatoes, has not yet been studied. To this end, we used the lambda recombineering method to generate a fur mutant strain of Pcb1692 and assessed the virulence and fitness of the mutant strain. The results showed that production of siderophores in Pcb1692Δfur increased compared to the Pcb1692 wild-type and the complemented strain Pcb1692Δfur-pfur. However, production of N-acyl homoserine lactone (AHLs), biofilm formation, exopolysaccharide (EPS) production, virulence on potato tubers and swimming motility, were all significantly decreased in Pcb1692Δfur compared to the wild-type and complemented Pcb1692Δfur-pfur strains. The Pcb1692Δfur mutant also demonstrated significant sensitivity to oxidative stress when exposed to H2O2. Consistent with phenotypic results, qRT-PCR results demonstrated that Fur down-regulates genes which encode proteins associated with: iron uptake (HasA-extracellular heme-binding protein and Ferrodoxin-AED-0004132), stress response (SodC-superoxide dismutase), plant cell wall degrading enzymes (PrtA and CelV) and motility (FlhC and MotA). We conclude that the ferric uptake regulator protein (Fur) of Pcb1692 regulates traits that are important to host-pathogens interactions.
Assuntos
Proteínas de Bactérias/metabolismo , Pectobacterium carotovorum/genética , Pectobacterium carotovorum/patogenicidade , Proteínas Repressoras/metabolismo , 4-Butirolactona/análogos & derivados , 4-Butirolactona/metabolismo , Proteínas de Bactérias/genética , Biofilmes/crescimento & desenvolvimento , Regulação para Baixo , Interações Hospedeiro-Patógeno , Peróxido de Hidrogênio/toxicidade , Ferro/metabolismo , Mutagênese , Estresse Oxidativo/efeitos dos fármacos , Pectobacterium carotovorum/metabolismo , Proteínas Repressoras/genética , Sideróforos/metabolismo , Solanum tuberosum/microbiologia , Superóxido Dismutase/metabolismo , Virulência/genéticaRESUMO
Soft rot is a commonly occurring potato tuber disease that each year causes substantial losses to the food industry. Here, we explore the possibility of early detection of the disease via gas/vapor analysis, in a laboratory environment, using a recent technology known as FAIMS (Field Asymmetric Ion Mobility Spectrometry). In this work, tubers were inoculated with a bacterium causing the infection, Pectobacterium carotovorum, and stored within set environmental conditions in order to manage disease progression. They were compared with controls stored in the same conditions. Three different inoculation time courses were employed in order to obtain diseased potatoes showing clear signs of advanced infection (for standard detection) and diseased potatoes with no apparent evidence of infection (for early detection). A total of 156 samples were processed by PCA (Principal Component Analysis) and k-means clustering. Results show a clear discrimination between controls and diseased potatoes for all experiments with no difference among observations from standard and early detection. Further analysis was carried out by means of a statistical model based on LDA (Linear Discriminant Analysis) that showed a high classification accuracy of 92.1% on the test set, obtained via a LOOCV (leave-one out cross-validation).
Assuntos
Espectrometria de Massas/métodos , Pectobacterium carotovorum/isolamento & purificação , Pectobacterium carotovorum/metabolismo , Doenças das Plantas/microbiologia , Tubérculos/microbiologia , Solanum tuberosum/microbiologia , Compostos Orgânicos Voláteis/análise , Biomarcadores/análiseRESUMO
Pectobacterium species are enterobacterial plant-pathogens that cause soft rot disease in diverse plant species. Unlike hemi-biotrophic plant pathogenic bacteria, the type III secretion system (T3SS) of Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) appears to secrete only one effector protein, DspE. Previously, we found that the T3SS regulator HrpL and the effector DspE are required for P. carotovorum pathogenesis on leaves. Here, we identified genes up-regulated by HrpL, visualized expression of dspE in leaves, and established that DspE causes host cell death. DspE required its full length and WxxxE-like motifs, which are characteristic of the AvrE-family effectors, for host cell death. We also examined expression in plant leaves and showed that hrpL is required for the expression of dspE and hrpN, and that the loss of a functional T3SS had unexpected effects on expression of other genes during leaf infection. These data support a model where P. carotovorum uses the T3SS early in leaf infection to initiate pathogenesis through elicitation of DspE-mediated host cell death.
Assuntos
Proteínas de Bactérias/genética , Cromossomos Bacterianos/química , Regulação Bacteriana da Expressão Gênica , Interações Hospedeiro-Patógeno , Pectobacterium carotovorum/genética , Solanum tuberosum/microbiologia , Fatores de Virulência/genética , Agrobacterium tumefaciens/genética , Agrobacterium tumefaciens/metabolismo , Motivos de Aminoácidos , Arabidopsis/microbiologia , Proteínas de Bactérias/metabolismo , Morte Celular , Ilhas Genômicas , Dados de Sequência Molecular , Pectobacterium carotovorum/metabolismo , Pectobacterium carotovorum/patogenicidade , Células Vegetais/metabolismo , Células Vegetais/microbiologia , Células Vegetais/patologia , Doenças das Plantas/microbiologia , Folhas de Planta/microbiologia , Alinhamento de Sequência , Fatores de Tempo , Nicotiana/microbiologia , Fatores de Virulência/metabolismoRESUMO
Several Gram-negative pathogenic bacteria employ N-acyl-L-homoserine lactone (HSL) quorum sensing (QS) system to control their virulence traits. Degradation of acyl-HSL signal molecules by quorum quenching enzyme (QQE) results in a loss of pathogenicity in QS-dependent organisms. The QQE activity of actinomycetes in rhizospheric soil and inside plant tissue was explored in order to obtain novel strains with high HSL-degrading activity. Among 344 rhizospheric and 132 endophytic isolates, 127 (36.9%) and 68 (51.5%) of them, respectively, possessed the QQE activity. The highest HSL-degrading activity was at 151.30 ± 3.1 nmole/h/mL from an endophytic actinomycetes isolate, LPC029. The isolate was identified as Streptomyces based on 16S rRNA gene sequence similarity. The QQE from LPC029 revealed HSL-acylase activity that was able to cleave an amide bond of acyl-side chain in HSL substrate as determined by HPLC. LPC029 HSL-acylase showed broad substrate specificity from C6- to C12-HSL in which C10HSL is the most favorable substrate for this enzyme. In an in vitro pathogenicity assay, the partially purified HSL-acylase efficiently suppressed soft rot of potato caused by Pectobacterium carotovorum ssp. carotovorum as demonstrated. To our knowledge, this is the first report of HSL-acylase activity derived from an endophytic Streptomyces.
Assuntos
Acil-Butirolactonas/metabolismo , Amidoidrolases/metabolismo , Proteínas Fúngicas/metabolismo , Raízes de Plantas/microbiologia , Rizosfera , Solanum tuberosum/microbiologia , Streptomyces/enzimologia , Pectobacterium carotovorum/metabolismo , RNA Fúngico/genética , RNA Ribossômico 16S/genética , Microbiologia do Solo , Streptomyces/genética , Streptomyces/isolamento & purificaçãoRESUMO
Bacteria obtained from potato tubers having symptoms of soft rot and grown in different regions of Ukraine are identified as Pectobacterium carotovorum subsp. carotovorum. These bacteria strains are able to produce bacteriocines. Their killer activity in respect of P. carotovorum and Esherichia coli has been studied. The sensitivity to bactericines has been shown. Purified fractions of bacteriocines having high molecular weight (MCTV) have been obtained. The difference in composition of proteins from phage tails as compared to the ones in P. carotovorum J2 has been studied by the method of electrophoresis. It was found that the composition of MCTV major proteins of studied isolates mostly corresponds to P. carotovorum J2. The set of enzyme minor fractions has some different compositions as compared to P. carotovorum J2. It has been hypothesized that this difference is responsible for killer specificity.
Assuntos
Bacteriocinas/biossíntese , Pectobacterium carotovorum/isolamento & purificação , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Bacteriocinas/isolamento & purificação , Bacteriocinas/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Microscopia Eletrônica , Peso Molecular , Pectobacterium carotovorum/crescimento & desenvolvimento , Pectobacterium carotovorum/metabolismo , Pectobacterium carotovorum/ultraestrutura , UcrâniaRESUMO
A total of 91 isolates of probable antagonistic bacteria of potato soft rot bacterium Erwinia carotovora subsp. carotovora (Ecc) were extracted from rhizospheres and endophytes of various crop plants, different soil varieties, and atmospheres in the potato farming areas of Bangladesh. Antibacterial activity of the isolated probable antagonistic bacteria was tested in vitro against the previously identified most common and most virulent soft rot causing bacterial strain Ecc P-138. Only two isolates E-45 and E-65 significantly inhibited the in vitro growth of Ecc P-138. Physiological, biochemical, and carbon source utilization tests identified isolate E-65 as a member of the genus Bacillus and the isolate E-45 as Lactobacillus sp. The stronger antagonistic activity against Ecc P-138 was found in E-65 in vitro screening and storage potatoes. E-65 reduced the soft rot infection to 22-week storage potatoes of different varieties by 32.5-62.5% in model experiment, demonstrating its strong potential to be used as an effective biological control agent for the major pectolytic bacteria Ecc. The highest (62.5%) antagonistic effect of E-65 was observed in the Granola and the lowest (32.7%) of that was found in the Cardinal varieties of the Bangladeshi potatoes. The findings suggest that isolate E-65 could be exploited as a biocontrol agent for potato tubers.
Assuntos
Agentes de Controle Biológico , Pectobacterium carotovorum/metabolismo , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Agricultura/métodos , Bacillus/metabolismo , Bangladesh , Bioquímica/métodos , Doenças das Plantas/terapia , Rizosfera , Solo , Especificidade da EspécieRESUMO
AIMS: The early molecular events underlying the elicitation of plant defence reactions by Gram-positive bacteria are relatively unknown. In plants, calcium and reactive oxygen species are commonly involved as cellular messengers of a wide range of biotic stimuli from pathogenic to symbiotic bacteria. In the present work, we checked whether nonpathogenic Streptomyces sp. strains could induce early signalling events leading to defence responses in BY2 tobacco cell suspensions. METHODS AND RESULTS: We have demonstrated that nonpathogenic Streptomyces sp. OE7 strain induced a cytosolic Ca(2+) increase and a biphasic oxidative burst in the upstream signalling events, leading to defence responses in BY2 tobacco cell suspensions. Streptomyces sp. OE7 also elicited delayed intracellular free scopoletin production and programmed cell death. In agreement with scopoletin production, OE7 induced accumulation of PAL transcripts and increased accumulation of transcripts of EREBP1 and AOX genes that are known to be regulated by the jasmonate/ethylene pathway. Transcript levels of PR1b and NIMIN2α, both salicylic acid pathway-linked genes, were not modified. Moreover, Streptomyces sp. OE7 culture filtrates could reduce Pectobacterium carotovorum- and Pectobacterium atrosepticum-induced death of BY2 cells and soft rot on potato slices. CONCLUSIONS: New insights are thus provided into the interaction mechanisms between Streptomyces sp. and plants; Streptomyces sp. could be sensed by plant cells, and through cytosolic Ca(2+) changes and the generation of reactive oxygen species, defence responses were induced. SIGNIFICANCE AND IMPACT OF THE STUDY: These induced defence responses appeared to participate in attenuating Pectobacterium-induced diseases in plants. Thus, Streptomyces sp. OE7 could be a biocontrol agent against Pectobacterium sp.
Assuntos
Cálcio/metabolismo , Nicotiana/metabolismo , Nicotiana/microbiologia , Espécies Reativas de Oxigênio/metabolismo , Apoptose , Pectobacterium/metabolismo , Pectobacterium carotovorum/metabolismo , Células Vegetais/imunologia , Células Vegetais/metabolismo , Células Vegetais/microbiologia , Escopoletina/metabolismo , Transdução de Sinais , Solanum tuberosum/metabolismo , Streptomyces/metabolismo , Streptomyces/patogenicidade , Nicotiana/citologia , Nicotiana/imunologiaRESUMO
Pectobacterium species are necrotrophic bacterial pathogens that cause soft rot diseases in potatoes and several other crops worldwide. Gene expression data identified Pectobacterium carotovorum subsp. carotovorum budB, which encodes the α-acetolactate synthase enzyme in the 2,3-butanediol pathway, as more highly expressed in potato tubers than potato stems. This pathway is of interest because volatiles produced by the 2,3-butanediol pathway have been shown to act as plant growth promoting molecules, insect attractants, and, in other bacterial species, affect virulence and fitness. Disruption of the 2,3-butanediol pathway reduced virulence of P. c. subsp. carotovorum WPP14 on potato tubers and impaired alkalinization of growth medium and potato tubers under anaerobic conditions. Alkalinization of the milieu via this pathway may aid in plant cell maceration since Pectobacterium pectate lyases are most active at alkaline pH.
Assuntos
Acetoína/metabolismo , Vias Biossintéticas , Pectobacterium carotovorum/metabolismo , Pectobacterium carotovorum/patogenicidade , Acetoína/farmacologia , Álcalis , Vias Biossintéticas/efeitos dos fármacos , Butileno Glicóis/farmacologia , Meios de Cultura/farmacologia , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Genes Bacterianos/genética , Concentração de Íons de Hidrogênio/efeitos dos fármacos , Mutação/genética , Óperon/genética , Pectobacterium carotovorum/genética , Pectobacterium carotovorum/crescimento & desenvolvimento , Caules de Planta/efeitos dos fármacos , Caules de Planta/microbiologia , Tubérculos/efeitos dos fármacos , Tubérculos/microbiologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Solanum tuberosum/efeitos dos fármacos , Solanum tuberosum/microbiologia , Técnicas de Cultura de Tecidos , Virulência/efeitos dos fármacosRESUMO
Integrating microbial physiology and biomass stoichiometry opens far-reaching possibilities for linking microbial dynamics to ecosystem processes. For example, the growth-rate hypothesis (GRH) predicts positive correlations among growth rate, RNA content, and biomass phosphorus (P) content. Such relationships have been used to infer patterns of microbial activity, resource availability, and nutrient recycling in ecosystems. However, for microorganisms it is unclear under which resource conditions the GRH applies. We developed a model to test whether the response of microbial biomass stoichiometry to variable resource stoichiometry can be explained by a trade-off among cellular components that maximizes growth. The results show mechanistically why the GRH is valid under P limitation but not under N limitation. We also show why variability of growth rate-biomass stoichiometry relationships is lower under P limitation than under N or C limitation. These theoretical results are supported by experimental data on macromolecular composition (RNA, DNA, and protein) and biomass stoichiometry from two different bacteria. In addition, compared to a model with strictly homeostatic biomass, the optimization mechanism we suggest results in increased microbial N and P mineralization during organic-matter decomposition. Therefore, this mechanism may also have important implications for our understanding of nutrient cycling in ecosystems.
Assuntos
Ecossistema , Escherichia coli/metabolismo , Modelos Biológicos , Pectobacterium carotovorum/metabolismo , Biomassa , Carbono/metabolismo , Simulação por Computador , Homeostase , Nitrogênio/metabolismo , Fósforo/metabolismoRESUMO
The carbon-use-efficiency (CUE) of microorganisms is an important parameter in determining ecosystem-level carbon (C) cycling; however, little is known about how variance in resources affects microbial CUE. To elucidate how resource quantity and resource stoichiometry affect microbial CUE, we cultured four microorganisms - two fungi (Aspergillus nidulans and Trichoderma harzianum) and two bacteria (Pectobacterium carotovorum and Verrucomicrobium spinosum) - under 12 unique C, nitrogen (N) and phosphorus (P) ratios. Whereas the CUE of A. nidulans was strongly affected by C, bacterial CUE was more strongly affected by mineral nutrients (N and P). Specifically, CUE in P. carotovorum was positively correlated with P, while CUE of V. spinosum primarily depended on N. This resulted in a positive relationship between fungal CUE and resource C : nutrient stoichiometry and a negative relationship between bacterial CUE and resource C : nutrient stoichiometry. The difference in the direction of the relationship between CUE and C : nutrient for fungi vs. bacteria was consistent with differences in biomass stoichiometry and suggested that fungi have a higher C demand than bacteria. These results suggest that the links between biomass stoichiometry, resource demand and CUE may provide a mechanism for commonly observed temporal and spatial patterns in microbial community structure and function in natural habitats.
Assuntos
Aspergillus nidulans/metabolismo , Carbono/metabolismo , Pectobacterium carotovorum/metabolismo , Trichoderma/metabolismo , Aspergillus nidulans/crescimento & desenvolvimento , Biomassa , Ecossistema , Nitrogênio/metabolismo , Pectobacterium carotovorum/crescimento & desenvolvimento , Fósforo/metabolismo , Trichoderma/crescimento & desenvolvimentoRESUMO
We isolated and identified AHL-degrading bacteria from the leaf surface of Solanum tuberosum. The 16 isolates inactivated both short- and long-chain AHLs. Two of these isolates, identified as Microbacterium testaceum, showed putative AHL-lactonase activity. These two strains interrupted quorum-sensing dependent bacterial infection by plant pathogen Pectobacterium carotovorum subsp. carotovorum. M. testaceum strains, which were isolated in this study, might be useful in the biocontrol of plant diseases.
Assuntos
4-Butirolactona/análogos & derivados , Bactérias Gram-Positivas/metabolismo , Pectobacterium carotovorum/metabolismo , Folhas de Planta/microbiologia , Percepção de Quorum , Solanum tuberosum/microbiologia , 4-Butirolactona/metabolismo , Sequência de Bases , Primers do DNA , Bactérias Gram-Positivas/enzimologia , Pectobacterium carotovorum/enzimologiaRESUMO
The exact function of type III secretion system in some phytopathogenes including Pectobacterium carotovorum subsp. atrosepticum (Pca) is not understood and is a matter of debate. The aim of this study were to determine specific effect of type III secretion system on potato tubers and to reveal the connection of this system with potato resistant genes such as PR-3. A Pca hrpW fragment was subcloned into a low-copy-number cloning vector (pZH448). The resulting plasmid (pAS19) was then conjugated into the wild-type and mutant strains of Pca by type III secretion system. The virulence property of different Pca strains was studied and the influence of over expression of hrpW on maceration activity was also investigated. Furthermore, the effect of mentioned mutation on the maceration of carrot-root was evaluated. Finally, using real-time PCR, the copy-number of PR-3 gene in potato tuber tissue was assessed. In conclusion, for type III secretion system mutant strains, in contrast with the wild-type, the maceration amount of potato tuber tissue decreased after over expression of hrpW while inoculation of tubers by mutants, increased this amount. In the case of potato, HrpN and DspE proteins appeared to be avirulent factors. Compared with the wild-type strains, Pca nominated mutants significantly reduced potato PR-3 expression thus, PR-3 expression level in potato tuber tissue in answer to infiltration by Pca, depends on functional type III secretion system in the bacterium.
Assuntos
Regulação da Expressão Gênica de Plantas , Pectobacterium carotovorum/genética , Pectobacterium carotovorum/patogenicidade , Solanum tuberosum/genética , Solanum tuberosum/microbiologia , Daucus carota/genética , Daucus carota/microbiologia , Genes Bacterianos , Genes de Plantas , Mutação , Pectobacterium carotovorum/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/microbiologia , Tubérculos/genética , Tubérculos/microbiologia , Solanum tuberosum/anatomia & histologia , Transformação BacterianaRESUMO
Pectobacterium atrosepticum is a Gram-negative plant-pathogenic bacterium that rots potato stems and tubers. Microarray analysis was used to identify genes that were differentially expressed when host extracts were added to the growth medium. Potato extracts downregulated the expression of ribosomal genes and genes related to uptake and metabolism of nutrients, and upregulated genes needed for nitrate or phosphonate use. Some of the observed changes in gene expression in host-extract-induced cultures are similar to those during attachment of the bacterium to host tissues. Other responses indicated defence against toxic metabolites in the extract. Tuber extract induced a large gene cluster having homology to type VI secretion genes shown to be virulence determinants in many, but not all, animal and human pathogens. Two of the genes in the type VI cluster were found to be expressed during infection in potato tubers and stems, and mutants with knockouts of the corresponding genes had increased virulence on potato. One of the type VI secretion mutants was further characterized and found to grow to higher cell density in culture in the presence of host extract and to produce slightly more extracellular tissue-macerating enzymes than the wild-type strain. Analysis of secreted proteins showed that this type VI mutant was affected in the production of haemolysin-coregulated proteins (Hcps), which have been suggested to be secreted by the type VI pathway in other bacteria. The results suggest that the type VI secretion system of P. atrosepticum is needed for secretion of Hcps but not for virulence on its host plant, potato.
Assuntos
Proteínas de Bactérias/genética , Interações Hospedeiro-Patógeno , Família Multigênica , Pectobacterium carotovorum/genética , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Proteínas de Bactérias/metabolismo , Transporte Biológico , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Pectobacterium carotovorum/metabolismo , FenótipoRESUMO
AHL-lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus thuringiensis, Bacillus cereus and Bacillus anthracis, specifically hydrolyzes N-acyl-homoserine lactones (AHLs) secreted by Gram-negative bacteria and thereby attenuates the symptoms caused by plant pathogens. In this study, an aiiA gene was cloned from Bacillus subtilis BS-1 by PCR with a pair of degenerate primers. The deduced 250 amino acid sequence contained two small conserved regions, 103SHLHFDH109 and 166TPGHTPGH173, which are characteristic of the metallo-beta-lactamase family. Homology comparison revealed that the deduced amino acid sequence had a high degree of similarity with those of the known AiiA proteins in the B. cereus group. Additionally, the aiiA gene was expressed in Escherichia coli BL21 (DE3) pLysS and the expressed AiiA protein could attenuate the soft rot symptoms caused by Erwinia carotovora var. carotovora.
Assuntos
Bacillus subtilis/enzimologia , Proteínas de Bactérias , Regulação Bacteriana da Expressão Gênica , Metaloendopeptidases , Pectobacterium carotovorum/crescimento & desenvolvimento , Controle Biológico de Vetores , Solanum tuberosum/microbiologia , Sequência de Aminoácidos , Bacillus subtilis/genética , Bacillus subtilis/crescimento & desenvolvimento , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Escherichia coli/enzimologia , Escherichia coli/genética , Metaloendopeptidases/química , Metaloendopeptidases/genética , Metaloendopeptidases/metabolismo , Dados de Sequência Molecular , Pectobacterium carotovorum/metabolismo , Doenças das Plantas/microbiologiaRESUMO
Erwinia carotovora subspecies betavasculorum, also known as E. betavasculorum and Pectobacterium betavasculorum, is a soil bacterium that has the capacity to cause root rot necrosis of sugarbeets. The qualitatively different pathogenicity exhibited by the virulent E. carotovora strain and two avirulent strains, a Citrobacter sp. and an Enterobacter cloacae, was examined using digital analysis of photographic evidence of necrosis as well as for carbohydrate, ethane, and ethylene release compared with uninoculated potato tuber slices. Visual scoring of necrosis was superior to digital analysis of photographs. The release of carbohydrates and ethane from potato tuber slices inoculated with the soft rot necrosis-causing Erwinia was significantly greater than that of potato tuber slices that had not been inoculated or that had been inoculated with the nonpathogenic E. cloacae and Citrobacter sp. strains. Interestingly, ethylene production from potato slices left uninoculated or inoculated with the nonpathogenic Citrobacter strain was 5- to 10-fold higher than with potato slices inoculated with the pathogenic Erwinia strain. These findings suggest that (1) carbohydrate release might be a useful measure of the degree of pathogenesis, or relative virulence; and that (2) bacterial suppression of ethylene formation may be a critical step in root rot disease formation.
Assuntos
Metabolismo dos Carboidratos , Etano/metabolismo , Pectobacterium carotovorum/metabolismo , Doenças das Plantas/microbiologia , Bioensaio/métodos , Cromatografia Gasosa/métodos , Citrobacter/metabolismo , Enterobacter cloacae/metabolismo , Etilenos/metabolismo , Pectobacterium carotovorum/patogenicidade , Fotografação , Solanum tuberosum/microbiologia , VirulênciaRESUMO
Many Gram-negative bacteria use N-acyl homoserine lactone signal molecules to monitor their own population density and coordinate gene regulation in a process called quorum sensing (QS). Increasing evidence implies that certain eukaryotes produce QS-inhibitory compounds. In this work, we tested 46 terrestrial plants materials for their ability to inhibit QS-regulated behaviors in different bacterial species. Plant materials were dried and extracted using different solvents. The chloroform-soluble compounds extracted from Scorzonera sandrasica were found to inhibit violacein production, a QS-regulated behavior in Chromobacterium violaceum. In addition, the chloroform extract was also able to inhibit QS-regulated carbapenem antibiotic production in Erwinia carotovora. Because the regulation of many bacterial processes is controlled by QS systems, the finding of natural compounds acting as QS inhibitors suggests an attractive tool to control and handle detrimental infections caused by human, animal, and plant pathogens.