RESUMO
Salvianolic acids (SA), such as rosmarinic acid (RA), danshensu (DSS), and their derivative salvianolic acid B (SAB), etc. widely existed in Lamiaceae and Boraginaceae families, are of interest due to medicinal properties in the pharmaceutical industries. Hundreds of studies in past decades described that 4-coumaroyl-CoA and 4-hydroxyphenyllactic acid (4-HPL) are common substrates to biosynthesize SA with participation of rosmarinic acid synthase (RAS) and cytochrome P450 98A (CYP98A) subfamily enzymes in different plants. However, in our recent study, several acyl donors and acceptors included DSS as well as their ester-forming products all were determined in SA-rich plants, which indicated that previous recognition to SA biosynthesis is insufficient. Here, we used Salvia miltiorrhiza, a representative important medicinal plant rich in SA, to elucidate the diversity of SA biosynthesis. Various acyl donors as well as acceptors are catalysed by SmRAS to form precursors of RA and two SmCYP98A family members, SmCYP98A14 and SmCYP98A75, are responsible for different positions' meta-hydroxylation of these precursors. SmCYP98A75 preferentially catalyses C-3' hydroxylation, and SmCYP98A14 preferentially catalyses C-3 hydroxylation in RA generation. In addition, relative to C-3' hydroxylation of the acyl acceptor moiety in RA biosynthesis, SmCYP98A75 has been verified as the first enzyme that participates in DSS formation. Furthermore, SmCYP98A enzymes knockout resulted in the decrease and overexpression leaded to dramatic increase of SA accumlation. Our study provides new insights into SA biosynthesis diversity in SA-abundant species and versatility of CYP98A enzymes catalytic preference in meta-hydroxylation reactions. Moreover, CYP98A enzymes are ideal metabolic engineering targets to elevate SA content.
Assuntos
Sistema Enzimático do Citocromo P-450 , Salvia miltiorrhiza , Hidroxilação , Sistema Enzimático do Citocromo P-450/metabolismo , Sistema Enzimático do Citocromo P-450/genética , Salvia miltiorrhiza/metabolismo , Salvia miltiorrhiza/genética , Salvia miltiorrhiza/enzimologia , Polifenóis/metabolismo , Polifenóis/biossíntese , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , AlcenosRESUMO
Selenium (Se) supplement was combined with different LED light qualities to investigate mutual effects on the growth, nutritional quality, contents of glucosinolates and mineral elements in broccoli sprouts. There were five treatments: CK:1R1B1G, 1R1B1G+Se (100 µmol L-1 Na2SeO3), 1R1B+Se, 1R2B+Se, 2R1B+Se, 60 µmol m-2 s-1 PPFD, 12 h/12 h (light/dark). Sprouts under a combination of selenium and LED light quality treatment exhibited no remarkable change fresh weight, but had a shorter hypocotyl length, lower moisture content and heavier dry weight, especially with 1R2B+Se treatment. The contents of carotenoid, soluble protein, soluble sugar, vitamin C, total flavonoids, total polyphenol and contents of total glucosinolates and organic Se were dramatically improved through the combination of Se and LED light quality. Moreover, heat map and principal component analysis showed that broccoli sprouts under 1R2B+Se treatment had higher nutritional quality and health-promoting compound contents than other treatments. This suggests that the Se supplement under suitable LED lights might be beneficial to selenium-biofortified broccoli sprout production.
Assuntos
Brassica/crescimento & desenvolvimento , Proteínas/metabolismo , Plântula/crescimento & desenvolvimento , Selênio/farmacologia , Ácido Ascórbico/biossíntese , Brassica/efeitos dos fármacos , Brassica/metabolismo , Brassica/efeitos da radiação , Carotenoides/metabolismo , Flavonoides/biossíntese , Glucosinolatos/biossíntese , Humanos , Luz , Polifenóis/biossíntese , Plântula/efeitos dos fármacos , Plântula/efeitos da radiação , Selênio/metabolismo , Açúcares/metabolismoRESUMO
This study was to obtain stable transformed roots of Salvia bulleyana using A. rhizogenes strain A4 and then evaluate their phytochemical profile and selected the most productive clone. Our results indicated that the type of explant and medium used for bacterium and explant incubation had an influence on the frequency of hairy root formation. The best response was obtained on leaves infected with bacteria cultivated on YMB medium supplemented with acetosyringone. Of the four selected transformed root clones, after five-week cultivation in Woody Plant (WP) medium, the highest growth indexes were demonstrated for line C1: i.e. 13 for fresh and 15 for dry weight (81.4 and 8.2â¯g/l fresh and dry weight, respectively). The qualitative analysis of hydromethanolic extracts of hairy roots of S. bulleyana using UPLC-PDA-ESI-MS/MS method showed the presence of 10 polyphenolic compounds including predominant rosmarinic acid (RA), its derivatives (hexoside and methyl rosmarinate), caffeic acid, its derivatives and several salvianolic acids: K, E and F. Their production varied among the four root clones studied; the highest RA (39.6â¯mg/g dry weight) and total polyphenol (48.9â¯mg/g dry weight) level were found in the roots of C4 clone. These values were significantly higher than those of the roots of plants grown for several years under field conditions. The transformation of the obtained root cultures was confirmed by polymerase chain reaction using aux1, aux2, rolB, rolC and rolD primers.
Assuntos
Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Polifenóis/biossíntese , Salvia , Agrobacterium/genética , Técnicas de Cultura de Células , Extratos Vegetais/química , Raízes de Plantas/química , Raízes de Plantas/genética , Plantas Geneticamente Modificadas , Polifenóis/química , Transformação GenéticaRESUMO
Dr. Ragai K. Ibrahim, Professor Emeritus at Concordia University, Montréal, Canada, passed away on the November 19, 2017 at the age of 88 years. Dr. Ibrahim dedicated his entire professional life to polyphenols and spent most of his academic career (1967-1997) at the Department of Biology of Concordia University in Montréal. He has been an active member of the Groupe Polyphénols since the beginning. This paper is a tribute to Dr. Ibrahim from some of his former students. An overview of the evolution of polyphenol research since the late 1950s and the outstanding contribution that Dr. Ibrahim had to this topic is given. The input of Dr. Ibrahim's research to the enzymology and genetics of polyphenol biosynthesis is discussed. Furthermore, the links between Dr. Ibrahim's work and some aspects of modern studies on the health benefits of polyphenols are presented.
Assuntos
Extratos Vegetais/biossíntese , Plantas/metabolismo , Polifenóis/biossíntese , Canadá , História do Século XX , História do Século XXI , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Plantas/química , Polifenóis/química , Polifenóis/farmacologiaRESUMO
Leaves are one of the most important organs of plants, and yet, the association between leaf color and consumable traits remains largely unclear. Tea leaves are an ideal study system with which to investigate the mechanism of how leaf coloration affects palatability, since tea is made from the leaves of the crop Camellia sinensis. Our genomic resequencing analysis of a tea cultivar ZiJuan (ZJ) with purple leaves and altered flavor revealed genetic variants when compared with the green-leaf, wild type cultivar YunKang(YK). RNA-Seq based transcriptomic comparisons of the bud and two youngest leaves in ZJ and YK identified 93%, 9% and 5% expressed genes that were shared in YK- and ZJ-specific cultivars, respectively. A comparison of both transcript abundance and particular metabolites revealed that the high expression of gene UFGT for anthocyanin biosynthesis is responsible for purple coloration, which competes with the intermediates for catechin-like flavanol biosynthesis. Genes with differential expression are enriched in response to stress, heat and defense, and are casually correlated with the environmental stress of ZJ plant origin in the Himalayas. In addition, the highly expressed C4H and LDOX genes for synthesizing flavanol precursors, ZJ-specific CLH1 for degrading chlorophyll, alternatively spliced C4H and FDR and low photosynthesis also contributed to the altered color and flavor of ZJ. Thus, our study provides a better molecular understanding of the effect of purple coloration on leaf flavor, and helps to guide future engineering improvement of palatability.
Assuntos
Camellia sinensis/fisiologia , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/genética , Processamento Alternativo , Antocianinas/biossíntese , Bioengenharia , Vias Biossintéticas/genética , Catequina/análogos & derivados , Catequina/biossíntese , Cor , Resposta ao Choque Térmico/genética , Metabolômica , Fotossíntese/genética , Melhoramento Vegetal/métodos , Folhas de Planta/química , Folhas de Planta/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Polimorfismo Genético , Polifenóis/biossíntese , RNA-Seq , Paladar , Chá/química , Transcriptoma/genéticaRESUMO
In this study, the potential of the pyrolysis method to overcome the negative effects of Azolla-filiculoides in infected areas was thoroughly investigated. Non-catalytic pyrolysis experiments were conducted at a temperature range of 400-700⯰C. The highest possible bio-oil yield (35â¯wt%) was attained at 500⯰C. To achieve the best chemical composition of bio-oil and higher amount of synthesis gas the catalytic pyrolysis were conducted in a dual-bed quartz reactor at the optimum temperature (500⯰C). Although, all three catalysts (pyro-char, modified pyro-char (MPC), and Mg-Ni-Mo/MPC) showed almost an impressive performance in promotion of the common reactions, Mg-Ni-Mo/MPC catalyst have illustrated the stunning results by increasing the percentage of furan compounds from 5.25% to 33.07%, and decreasing the acid compounds from 25.56% to 9.09%. Using GC-MS and GC-FID liquid and gaseous products were fully analyzed. The carbon-based catalysts were also evaluated via FTIR, FESEM, EDX, and BET analyses.
Assuntos
Polifenóis/biossíntese , Traqueófitas/metabolismo , Biocatálise , Cromatografia Gasosa-Espectrometria de Massas , Temperatura Alta , Óleos de Plantas , PiróliseRESUMO
Tea plant (Camellia sinensis) is an important leaf-type woody crop used to produce non-alcoholic beverages all over the world. Tea is one of the oldest and most popular non-alcoholic beverages in the world, and long-term tea drinking has numerous healthful for humans due to many of the important secondary metabolites, such as polyphenols and theanine. Theanine and polyphenols are also closely related to tea flavor and tea aroma, which is usually as the standard for judging tea quality. The growth of tea plants and quality of teas are susceptible to adversity abiotic and biotic stresses, such as low temperatures and pests. Consequently, this review focus on the research progress of key genes related to the stress resistance and material metabolism of tea plants in recent years. We aim at comprehensively understanding the growth and metabolism of tea plants and their relationship with the external environment, so as to provide an in-depth and broad theoretical support for the breeding of excellent tea plant varieties.
Assuntos
Camellia sinensis/fisiologia , Proteínas de Plantas/genética , Camellia sinensis/genética , Regulação da Expressão Gênica de Plantas , Polifenóis/biossíntese , Estresse Fisiológico , Chá/química , Chá/genéticaRESUMO
Salvia species find widespread application in food and pharmaceutical products owing to their large polyphenol content. The main polyphenols in Chinese Salvia species are phenolic acids and flavonoids, which exhibit anti-oxygenation, anti-ischemia-reperfusion injury, anti-thrombosis, anti-tumour, and other therapeutic effects. However, there are few peer-reviewed studies on polyphenols in Chinese Salvia species, especially flavonoids. This review is a systematic, comprehensive collation of available information on the biosynthesis, chemistry, and pharmacology of Chinese Salvia species. We believe that our study makes a significant contribution to the literature because this review provides a detailed literary resource on the currently available information on various polyphenolic components of Chinese Salvia species, including their bioactivities and structures. In addition, the study provides information that would encourage further investigation of this plant material as a natural resource with potential for a broad range of applications in various industries, such as the food and pharmaceutical industries.
Assuntos
Extratos Vegetais/química , Polifenóis , Salvia miltiorrhiza/química , Animais , Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Avaliação Pré-Clínica de Medicamentos , Fibrinolíticos/farmacologia , Flavonoides/química , Humanos , Hidroxibenzoatos/química , Isquemia/tratamento farmacológico , Estrutura Molecular , Polifenóis/biossíntese , Polifenóis/isolamento & purificação , Polifenóis/farmacologia , Relação Estrutura-AtividadeRESUMO
In this study, co-liquefaction (HTL) of Prosopis juliflora (PJ) biomass with polyolefin waste (PO) was performed to produce bio-oil. HTL on bio-oil yield was studied at varying PJ to PO ratios (0:1, 1:0, 1:1, 2:1, 3:1, 4:1 and 5:1) and temperatures from 340 to 440⯰C. Bio-oil and HTL by-products were characterized by Mass Spectroscopy (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Bio-oil yield was around 61.23%wt at 420⯰C for 3:1 blends with 3â¯wt% of HCl activated bentonite catalyst at 60â¯min holding time. HHV value was 46â¯MJ/Kg with 88.23% purity (petro-diesel). Additionally gas possessed 26.28% of Hydrogen gas, 45.59% of Carbon dioxide gas, 7.1% of Carbon monoxide gas, 8.12% of Methane gas and other elements. The energy recovery (78%) and carbon recovery (94%) was higher for 3:1 blends bio-oil than PO and PJ processed bio-oils. HTL wastewater possessed higher degree of reusability nature as HTL medium.
Assuntos
Polienos/metabolismo , Prosopis/metabolismo , Biocombustíveis , Biomassa , Cromatografia Gasosa-Espectrometria de Massas , Hidrocarbonetos/metabolismo , Eliminação de Resíduos de Serviços de Saúde , Óleos de Plantas , Polifenóis/biossíntese , Espectroscopia de Infravermelho com Transformada de Fourier , TemperaturaRESUMO
The upsurge in the concerning issues like global warming, environmental pollution and depletion of fossil fuel resources led to the thrust on third generation biofuels. Algal research has gained a lot of importance in the recent years. Effective utilization of algal biomass in a single step is necessary as it can produce Bio-oil (BO), gases and in addition to a variety of valuable products, along with nutrient recovery. Hydrothermal liquefaction technology does not require the energy intensive drying steps and is an attractive approach for the conversion of algae to liquid fuels. This study investigates direct hydrothermal liquefaction (HTL) of microalgae (Algal biomass) to produce bio-oil using a high-pressure batch reactor under subcritical water conditions. Three different micro algae samples namely, Chlorella vulgaris, Botryococcus braunii and Scenedesmus quadricauda have been examined under hydrothermal liquefaction with different water concentrations (1:6, 1:7, 1:8, 1:9 & 1:10 ratio) at certain temperature range (200-320⯰C), pressure (60 bars) and reaction time (30â¯min). Through liquefaction, the highest BO yield achieved with S. quadricauda was 18â¯wt% at 1:9 ratio. The chemical components of the obtained bio-oil were analyzed via gas chromatography and the results indicated that the algal BO was composed of furan, phenol, acid, and ester derivatives. Moreover, it was found that by increasing the temperatures, the BO yields increased. This was due to the polymerization reactions that converted the small biomass components into heavier molecules. FTIR spectra showed high percentage of Aliphatic, Phenolic, alcoholic, Carboxylic and Hydroxyl groups for solid residues.
Assuntos
Microalgas/metabolismo , Óleos de Plantas/química , Polifenóis/biossíntese , Polifenóis/química , Temperatura , Água/química , Biomassa , Chlorella vulgaris/metabolismo , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The aim of this study was the purification process of polyphenols from Aronia melanocarpa (chokeberry), and the purification parameters were optimised by adsorption and desorption tests. By comparing adsorption and desorption ability of polyphenols from chokeberry on six kinds of macroporous resin, XAD-7 resin was selected. Experiments prove that the best purification parameters of static adsorption and desorption were sample pH = 4.0 with 4 h of adsorption; and desorption solvent is 95% ethanol (pH = 7.0) with 2 h of desorption. The best dynamic parameters were 9.3 bed volume (BV) of sample loading amount at a feeding flow rate of 2 BV/h, and washing the column with 5.8 BV of water, followed by subsequent elution with an eluent volume of 5.0 mL at an elution flow rate of 2 BV/h. Next the antioxidant and antiproliferative activity of polyphenols from chokeberry, blueberries, haskap berries was studied on HepG2 human liver cancer cells. The results show that polyphenol from chokeberry has a strong antioxidant effect. Taking into account the content of polyphenols in fruit, polyphenols from chokeberry represent a very valuable natural antioxidant source with antiproliferative products.
Assuntos
Antioxidantes/química , Proliferação de Células/efeitos dos fármacos , Photinia/metabolismo , Extratos Vegetais/química , Polifenóis/isolamento & purificação , Resinas Acrílicas/química , Resinas Acrílicas/farmacologia , Adsorção , Antioxidantes/farmacologia , Mirtilos Azuis (Planta)/química , Mirtilos Azuis (Planta)/metabolismo , Sobrevivência Celular , Frutas/química , Frutas/metabolismo , Células Hep G2 , Humanos , Cinética , Extratos Vegetais/farmacologia , Polifenóis/biossíntese , Poliestirenos/química , Poliestirenos/farmacologiaRESUMO
BACKGROUND: Although Cannabis sativa L. is one of the most versatile plant species with multipurpose use both as medical, alimentary source and as psychoactive abuse, its biomedical relevance focused the attention on major cannabinoids. Phytochemical characterization of cannabis highlights the presence of various non-cannabinoids constituents including flavonoids, spiroindans, dihyrostilbenes, dihydrophenanthrenes, lignanamides, steroids and alkaloids. This review aims to identify polyphenols present in this plant, their biosynthesis, their bioactivities and their synthesis, when this occurred. METHODS: We undertook a systematic research focused on bibliographic databases including all noncannabinoids phenolics in various C. sativa strains from their isolation, structural elucidation, their biological activity to their synthesis. RESULT: Nevertheless, attention has so far been focused only on cannabinoids (more than one hundred isolated), cannabis is a complex plant able to produce more than 480 chemical entities that represent almost all of the different biogenetic classes. Regarding phenolic compounds, the plant biosynthesises a plethora of unique non-cannabinoids second metabolites, such as prenylated flavonoids, stilbenoids derivatives and lignanammides. CONCLUSION: Cannabis is a plant with high pharmacological and nutrition values, its potentialities and applications are not only circumscribed to cannabinoids biological activities, but also defined by noncannabinoid compounds. The combination of other cannabinoids together with noncannabinoid components could enhance the beneficial effects of THC and could reduce undesirable side effects.
Assuntos
Cannabis/química , Extratos Vegetais/química , Polifenóis/química , Polifenóis/farmacologia , Canabinoides/química , Canabinoides/farmacologia , Humanos , Estrutura Molecular , Polifenóis/biossíntese , Relação Estrutura-AtividadeRESUMO
Yarrowia lipolytica is considered as a promising microbial cell factory for bio-oil production due to its ability to accumulate a large amount of lipid. However, the regulation of lipid metabolism in this oleaginous yeast is elusive. In this study, the MHY1 gene was disrupted, and 43.1% (w/w) intracellular oil based on cell dry weight was obtained from the disruptant M-MHY1, while only 30.2% (w/w) lipid based on cell dry weight was obtained from the reference strain. RNA-seq was then performed to analyze transcriptional changes during lipid biosynthesis after MHY1 gene inactivation. The expression of 1597 genes, accounting for 24.7% of annotated Y. lipolytica genes, changed significantly in the disruptant M-MHY1 during lipid biosynthesis. Differential gene expression analysis indicated that Mhy1p performs multiple functions and participates in a wide variety of biological processes, including lipid, amino acid and nitrogen metabolism. Notably, data analysis revealed increased carbon flux through lipid biosynthesis following MHY1 gene inactivation, accompanied by decreased carbon flux through amino acid biosynthesis. Moreover, Mhy1p regulates the cell cycle, and the cell cycle rate was enhanced in the disruptant M-MHY1. These results suggest that Mhy1p plays critical regulatory roles in diverse aspects of various biological processes, especially in lipid biosynthesis, amino acid and nitrogen metabolism and cell cycle. Our dataset appears to elucidate the crucial role of Mhy1p in lipid biosynthesis and serves as a resource for exploring physiological dimorphic growth in Y. lipolytica.
Assuntos
Proteínas de Ligação a DNA/fisiologia , Proteínas Fúngicas/fisiologia , Metabolismo dos Lipídeos/genética , Yarrowia/genética , Yarrowia/metabolismo , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica , Lipídeos/biossíntese , Lipogênese/genética , Redes e Vias Metabólicas/genética , Organismos Geneticamente Modificados , Óleos de Plantas , Polifenóis/biossíntese , TranscriptomaRESUMO
Polyphenols are one of the largest groups of compounds that confer benefits to the health of plants and humans. Flavonol glycosides are a major ingredient of polyphenols in Camellia sinensis. Flavonol-3-O-glycosides are characteristic astringent taste compounds in tea infusion. A polyphenolic glycosyltransferase (CsUGT72AM1) belonging to cluster IIIb was isolated from the tea plant. The full-length cDNA of CsUGT72AM1 is 1416 bp. It encodes 472 amino acids with a calculated molecular mass of 50.92 kDa and an isoelectric point of 5.21. The recombinant CsUGT72AM1 protein was expressed in Escherichia coli and exhibited catalytic activity toward multiple flavonoids and coniferyl aldehyde. The enzyme assay indicated that rCsUGT72AM1 could perform glycosidation of flavonols or coniferyl aldehyde in vitro to form 3-O-glucoside or 4-O-glucoside, respectively. Interestingly, this enzyme also had activities and performed multisite glycosidation toward flavanones. The consistent products were confirmed to be naringenin-7-O-glucoside and -4'-O-glucoside by the nuclear magnetism assay. In addition, in the enzyme assay with cyanidin as the substrate, the results suggested that the glycosylated activity of CsUGT72AM1 was remarkably inhibited by a high concentration of anthocyanins. The above results indicate that CsUGT72AM1 may be involved in the metabolism of flavonol, flavanone, anthocyanin, and lignin.
Assuntos
Camellia sinensis/enzimologia , Glucosídeos/biossíntese , Glicosiltransferases/metabolismo , Proteínas de Plantas/metabolismo , Polifenóis/biossíntese , Camellia sinensis/genética , Camellia sinensis/metabolismo , Regulação da Expressão Gênica de Plantas , Glicosiltransferases/química , Glicosiltransferases/genética , Proteínas de Plantas/química , Proteínas de Plantas/genética , Especificidade por Substrato , Difosfato de Uridina/metabolismoRESUMO
Longan (Dimocarpus longan Lour.), an important subtropical fruit in the family Sapindaceae, is grown in more than 10 countries. Longan is an edible drupe fruit and a source of traditional medicine with polyphenol-rich traits. Tree size, alternate bearing, and witches' broom disease still pose serious problems. To gain insights into the genomic basis of longan traits, a draft genome sequence was assembled. The draft genome (about 471.88 Mb) of a Chinese longan cultivar, "Honghezi," was estimated to contain 31 007 genes and 261.88 Mb of repetitive sequences. No recent whole-genome-wide duplication event was detected in the genome. Whole-genome resequencing and analysis of 13 cultivated D. longan accessions revealed the extent of genetic diversity. Comparative transcriptome studies combined with genome-wide analysis revealed polyphenol-rich and pathogen resistance characteristics. Genes involved in secondary metabolism, especially those from significantly expanded (DHS, SDH, F3ÎH, ANR, and UFGT) and contracted (PAL, CHS, and F3Î5ÎH) gene families with tissue-specific expression, may be important contributors to the high accumulation levels of polyphenolic compounds observed in longan fruit. The high number of genes encoding nucleotide-binding site leucine-rich repeat (NBS-LRR) and leucine-rich repeat receptor-like kinase proteins, as well as the recent expansion and contraction of the NBS-LRR family, suggested a genomic basis for resistance to insects, fungus, and bacteria in this fruit tree. These data provide insights into the evolution and diversity of the longan genome. The comparative genomic and transcriptome analyses provided information about longan-specific traits, particularly genes involved in its polyphenol-rich and pathogen resistance characteristics.
Assuntos
Frutas/genética , Genoma de Planta , Sapindaceae/genética , Processamento Alternativo , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Filogenia , Polimorfismo de Nucleotídeo Único , Polifenóis/biossíntese , Análise de Sequência de RNARESUMO
Although the cause of Alzheimer's disease (AD) is unknown, glial-induced neuroinflammation is an early symptom. Familial AD is caused by increases in amyloid-beta (Aß) peptide, particularly soluble oligomeric (oAß), considered a proximal neurotoxin and neuroinflammatory stimuli. APOE4, a naturally occurring genotype of APOE, is the greatest genetic risk factor for AD; increasing risk up to 12-fold compared to APOE3 and APOE2. oAß-induced neuroinflammation is greater with APOE4 compared to APOE3 and APOE2. As sinapates and flavonoids have anti-inflammatory properties, a protocol was developed for optimizing polyphenol production in seedlings of Arabidopsis thaliana (A. thaliana). Three mutants (cop1, prn1, xpf3) were identified, and the extracts treated with liver microsomes to mimic physiological metabolism, with HPLC and MS performed on the resulting metabolites for peak identification. These extracts were used to treat primary glial cells isolated from human APOE-targeted-replacement (APOE-TR) and APOE-knock-out (KO) mice, with neuroinflammation induced by lipopolysaccharide (LPS) or oAß. The dose-response data for TNFα secretion demonstrate the followed the order: APOE-KO > APOE4 > APOE3 > APOE2, with xpf3 the most effective anti-neuroinflammatory across APOE genotypes. Thus, the plant-based approach described herein may be particularly valuable in treating the APOE4-induced neuroinflammatory component of AD risk.
Assuntos
Doença de Alzheimer/tratamento farmacológico , Apolipoproteínas E/efeitos adversos , Arabidopsis/metabolismo , Inflamação/prevenção & controle , Extratos Vegetais/metabolismo , Polifenóis/biossíntese , Polifenóis/uso terapêutico , Doença de Alzheimer/etiologia , Animais , Apolipoproteínas E/genética , Arabidopsis/efeitos da radiação , Relação Dose-Resposta a Droga , Genótipo , Técnicas In Vitro , Inflamação/etiologia , Lipopolissacarídeos/toxicidade , Camundongos , Camundongos Knockout , Extratos Vegetais/farmacologia , Fator de Crescimento Transformador alfa/metabolismo , Raios UltravioletaRESUMO
To clarify the mechanisms of selenium (Se) tolerance in peanut seedlings, we grew peanut seedlings with sodium selenite (0, 3, and 6 mg/L), and investigated the phenylpropanoids metabolism in seedling roots. The results showed that selenite up-regulated the expression of genes and related enzyme activities involving in the phenylpropanoids biosynthesis cascade, such as phenylalanine ammonia-lyase, trans-cinnamate-4-hydroxylase, chalcone synthase, chalcone isomerase, and cinnamyl-alcohol dehydrogenase. Selenite significantly increased phenolic acids and flavonoids, which contributed to the alleviation of selenite-induced stress. Moreover, selenite enhanced the formation of endodermis in roots, which may be attributed to the up-regulation of lignin biosynthesis mediated by the selenite-induced changes of H2O2 and NO, which probably regulated the selenite uptake from an external medium. Accumulation of polyphenolic compounds via the phenylpropanoid pathway may be one of the mechanisms of the increasing selenite tolerance in plants, by which peanut seedlings survived in seleniferous soil, accompanied by accumulation of Se.
Assuntos
Arachis/metabolismo , Polifenóis/biossíntese , Plântula/crescimento & desenvolvimento , Selênio/metabolismo , Aciltransferases/genética , Aciltransferases/metabolismo , Oxirredutases do Álcool/genética , Oxirredutases do Álcool/metabolismo , Arachis/enzimologia , Arachis/genética , Arachis/crescimento & desenvolvimento , Vias Biossintéticas , Regulação da Expressão Gênica de Plantas , Lignina/metabolismo , Fenilalanina Amônia-Liase/genética , Fenilalanina Amônia-Liase/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plântula/metabolismo , Ácido Selenioso/metabolismo , Transcinamato 4-Mono-Oxigenase/genética , Transcinamato 4-Mono-Oxigenase/metabolismoRESUMO
In recent years, more and more researches focus on endophytic fungi derived from important medicinal plants, which can produce the same bioactive metabolites as their host plants. Salvia miltiorrhiza Bunge is a traditional medicinal plant with versatile pharmacological effects. But the wild plant resource has been in short supply due to the overcollection for bioactive metabolites. Our study was therefore conducted to isolate endophytic fungi from S. miltiorrhiza and get candidate strains that produce the same bioactive compounds as the plant. As a result, an endophyte that produces salvianolic acid C was obtained and identified as Phoma glomerata D14 based on its morphology and internal transcribed spacer analysis. Salvianolic acid C was found present in both the mycelia and fermentation broth. Our study indicates that the endophytic fungus has significant industrial potential to meet the pharmaceutical demands for salvianolic acid C in a cost-effective, easily accessible, and reproducible way.
Assuntos
Ascomicetos/isolamento & purificação , Ascomicetos/metabolismo , Endófitos/isolamento & purificação , Endófitos/metabolismo , Polifenóis/biossíntese , Salvia miltiorrhiza/microbiologia , Alcenos , Ascomicetos/classificação , Ascomicetos/genética , Endófitos/classificação , Endófitos/genética , Micélio/metabolismoRESUMO
BACKGROUND: Effect of chlorocholine chloride (CCC) on phenolic acids composition and polyphenols accumulation in various anatomical parts (stems, leaves and inflorescences) of common buckwheat (Fagopyrum esculentum Moench) in the early stages of vegetation period were surveyed. RESULTS: Treatment of buckwheat seeds with 2% of CCC has been increased content of total phenolics in the stems, leaves and inflorescences. On analyzing the different parts of buckwheat plants, 9 different phenolic acids - vanilic acid, ferulic acid, trans-ferulic acid, chlorogenic acid, salycilic acid, cinamic acid, p-coumaric acid, p-anisic acid, methoxycinamic acid and catechins were identified. The levels of identified phenolic acids varied not only significantly among the plant organs but also between early stages of vegetation period. Same changes as in contents of chlorogenic acid, ferulic acid, trans-ferulic acid were found for content of salycilic acid. The content of these phenolic acids has been significant increased under effect of 2% CCC treatment at the phase I (formation of buds) in the stems and at the phase II (beginning of flowering) in the leaves and then inflorescences respectively. The content of catechins as potential buckwheat antioxidants has been increased at the early stages of vegetation period after treatment with 2% CCC. CONCLUSIONS: The obtained results suggest that influence of CCC on the phenolics composition can be a result of various mechanisms of CCC uptake, transforming and/or its translocation in the buckwheat seedlings.
Assuntos
Clormequat/farmacologia , Fagopyrum/efeitos dos fármacos , Hidroxibenzoatos/metabolismo , Polifenóis/biossíntese , Antioxidantes/análise , Antioxidantes/metabolismo , Catequina/análise , Ácido Clorogênico/análise , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/análise , Fagopyrum/crescimento & desenvolvimento , Fagopyrum/metabolismo , Éteres de Hidroxibenzoatos/análise , Hidroxibenzoatos/química , Inflorescência/efeitos dos fármacos , Inflorescência/metabolismo , Molibdênio , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo , Caules de Planta/efeitos dos fármacos , Caules de Planta/metabolismo , Propionatos , Sementes/efeitos dos fármacos , Sementes/metabolismo , Compostos de TungstênioRESUMO
BACKGROUND: Polyphenols have a favourable antioxidant potential on human health, suggesting that their high content in apple is responsible for the beneficial effects of apple consumption. They are also linked to the quality of apple juices and ciders since they are predominantly responsible for astringency, bitterness and colour. Major phenolic compounds were quantified by liquid chromatography in fruits and juices from a cider apple progeny harvested for 3 years. The total content of procyanidins and their average degree of polymerisation (DPn) were also determined in fruits by phloroglucinolysis. Variability and extraction yield of these compounds were determined. RESULTS: The variability observed in the progeny was representative of the variability observed in many cider apple varieties. Hydroxycinnamic acids were the most extractable group, with an average extraction yield of 67%, whereas flavonols and anthocyanins were the least. CONCLUSION: This study is the first to introduce variability and extraction yields of the main phenolic compounds in both fruits and juices of a cider apple progeny. This dataset will be used for an upcoming QTL mapping study, an original approach that has never been undertaken for cider apple.