RESUMO
The research objective was to study the presence of DNA damages in haddock exposed to petrogenic or pyrogenic polyaromatic hydrocarbons (PAHs) from different sources: 1) extracts of oil produced water (PW), dominated by 2-ring PAHs; 2) distillation fractions of crude oil (representing oil-based drilling mud), dominated by 3-ring PAHs; 3) heavy pyrogenic PAHs, mixture of 4/5/6-ring PAHs. The biological effect of the different PAH sources was studied by feeding juvenile haddock with low doses of PAHs (0.3-0.7 mg PAH/kg fish/day) for two months, followed by a two-months recovery. In addition to the oral exposure, a group of fish was exposed to 12 single compounds of PAHs (4/5/6-ring) via intraperitoneal injection. The main endpoint was the analysis of hepatic and intestinal DNA adducts. In addition, PAH burden in liver, bile metabolites, gene and protein expression of CYP1A, GST activity, lipid peroxidation, skeletal deformities and histopathology of livers were evaluated. Juvenile haddock responded quickly to both intraperitoneal injection and oral exposure of 4/5/6-ring PAHs. High levels of DNA adducts were detected in livers three days after the dose of the single compound exposure. Fish had also high levels of DNA adducts in liver after being fed with extracts dominated by 2-ring PAHs (a PW exposure scenario) and 3-ring PAHs (simulating an oil exposure scenario). Elevated levels of DNA adducts were observed in the liver of all exposed groups after the 2 months of recovery. High levels of DNA adduct were found also in the intestines of individuals exposed to oil or heavy PAHs, but not in the PW or control groups. This suggests that the intestinal barrier is very important for detoxification of orally exposures of PAHs.
Assuntos
Dano ao DNA , Gadiformes/crescimento & desenvolvimento , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Poluentes do Solo/toxicidade , Poluentes Químicos da Água/toxicidade , Administração Oral , Animais , Hidrocarboneto de Aril Hidroxilases/genética , Gadiformes/genética , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Infusões Parenterais , Intestinos/química , Fígado/química , Petróleo , Poluição por Petróleo , Hidrocarbonetos Policíclicos Aromáticos/administração & dosagem , Poluentes do Solo/administração & dosagem , Poluentes Químicos da Água/administração & dosagemRESUMO
The present study exposed adult zebrafish to 0, 10, and 100 µg/L perfluorobutanesulfonate (PFBS) with or without dietary supplement of probiotic Lactobacillus rhamnosus. Interaction between probiotic and PFBS on sex endocrine and reproduction was investigated. It was striking to find that PFBS and probiotic coexposures almost ceased the fecundity, which was accompanied by disturbances in sex hormones and oocyte maturation in females. In contrast, probiotic additive efficiently antagonized the estrogenic activity of PFBS in males. For the first time, this study reported that probiotic heavily depended on sex to modulate the endocrine disruption and reproductive toxicity of aquatic pollutants.
Assuntos
Disruptores Endócrinos/toxicidade , Fluorocarbonos/toxicidade , Probióticos/toxicidade , Reprodução/efeitos dos fármacos , Ácidos Sulfônicos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Suplementos Nutricionais , Disruptores Endócrinos/administração & dosagem , Estrogênios/metabolismo , Feminino , Fluorocarbonos/administração & dosagem , Hormônios Esteroides Gonadais/antagonistas & inibidores , Lacticaseibacillus rhamnosus/química , Masculino , Oócitos/efeitos dos fármacos , Probióticos/administração & dosagem , Ácidos Sulfônicos/administração & dosagem , Poluentes Químicos da Água/administração & dosagem , Peixe-ZebraRESUMO
Fish (embryo) toxicity test guidelines are mostly based on aquatic exposures. However, in some cases, other exposure routes can be more practical and relevant. Micro-injection into the yolk of fish embryos could offer a particular advantage for administering hydrophobic compounds, such as many endocrine disruptors. Single-dose micro-injection was compared with continuous aquatic exposure in terms of compound accumulation and biological responses. 17α-Ethinyl estradiol (EE2) was used as a model compound. First, the optimal solvent and droplet size were optimized, and needle variation was assessed. Next, biological endpoints were evaluated. The accumulated internal dose of EE2 decreased over time in both exposure scenarios. Estrogen receptor activation was concentration/injected dose dependent, increased daily, and was related to esr2b transcription. Transcription of vitellogenin 1 (vtg1) and brain aromatase (cyp19a1b) was induced in both scenarios, but the cyp19a1b transcription pattern differed between routes. Injection caused an increase in cyp19a1b transcripts from 48 hours post fertilization (hpf) onward, whereas after aquatic exposure the main increase occurred between 96 and 120 hpf. Some malformations only occurred after injection, whereas others were present for both scenarios. We conclude that responses can differ between exposure routes and therefore micro-injection is not a direct substitute for, but can be complementary to aquatic exposure. Nevertheless, vtg1and cyp19a1b transcription and estrogen receptor activation are suitable biomarkers for endocrine disruptor screening in both scenarios. Environ Toxicol Chem 2019;38:533-547. © 2018 SETAC.
Assuntos
Disruptores Endócrinos/administração & dosagem , Testes de Toxicidade/métodos , Poluentes Químicos da Água/administração & dosagem , Animais , Aromatase/genética , Aromatase/metabolismo , Embrião não Mamífero/efeitos dos fármacos , Disruptores Endócrinos/toxicidade , Etinilestradiol/administração & dosagem , Etinilestradiol/toxicidade , Masculino , Microinjeções/métodos , Receptores de Estrogênio/metabolismo , Vitelogeninas/genética , Vitelogeninas/metabolismo , Poluentes Químicos da Água/toxicidade , Peixe-Zebra/embriologia , Peixe-Zebra/metabolismo , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismoRESUMO
To demonstrate the effects of weathered crude oil residue on the immune systems of resident fish, we measured the changes in toxic chemical concentrations, apoptosis, phagocytosis, metabolism, immune-related gene expression, and cell cycle arrest in livers or kidneys for up to 96â¯h after the weathered Iranian heavy crude oil (WIHCO) exposure by oral gavage in juvenile rockfish Sebastes schlegeli. Parent polyaromatic hydrocarbons (PAHs) in livers increased up to 5590â¯ng/g after 6â¯h exposure and then declined rapidly within 24â¯h. Hepato-detoxification and immune-related gene expression were also significantly increased (Pâ¯<â¯0.05) after 6â¯h exposure and then declined rapidly within 24â¯h. However, biliary PAH metabolites and EROD activity remained elevated throughout the test period. Flow cytometry analysis also indicated sustained apoptosis and cell cycle arrests with reduced phagocytic activity for 96â¯h. Taken together, these results demonstrate rapid declination of the parent PAHs, whereas PAH metabolites remained much longer in tissues with prolonged suppression of immunity in molecular and cellular level, suggesting that weathered crude oil residue is likely linked to the high incidence of immune dysfunction in residential rockfish in oil spill area.
Assuntos
Bass/imunologia , Petróleo/toxicidade , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/toxicidade , Administração Oral , Animais , Bile/metabolismo , Biotransformação/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Perfilação da Expressão Gênica , Irã (Geográfico) , Rim/efeitos dos fármacos , Rim/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Poluição por Petróleo/efeitos adversos , Hidrocarbonetos Policíclicos Aromáticos/análise , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
Concerns exist as to whether individuals may be at greater risk for neurotoxicity following increased manganese (Mn) oral intake. The goals of this study were to determine the equivalence of 3 methods of oral exposure and the rate (mg Mn/kg/day) of exposure. Adult male rats were allocated to control diet (10 ppm), high manganese diet (200 ppm), manganese-supplemented drinking water, and manganese gavage treatment groups. Animals in the drinking water and gavage groups were given the 10 ppm manganese diet and supplemented with manganese chloride (MnCl(2)) in drinking water or once-daily gavage to provide a daily manganese intake equivalent to that seen in the high-manganese diet group. No statistically significant difference in body weight gain or terminal body weights was seen. Rats were anesthetized following 7 and 61 exposure days, and samples of bile and blood were collected. Rats were then euthanized and striatum, olfactory bulb, frontal cortex, cerebellum, liver, spleen, and femur samples were collected for chemical analysis. Hematocrit was unaffected by manganese exposure. Liver and bile manganese concentrations were elevated in all treatment groups on day 61 (relative to controls). Increased cerebellum manganese concentrations were seen in animals from the high-manganese diet group (day 61, relative to controls). Increased (relative to all treatment groups) femur, striatum, cerebellum, frontal cortex, and olfactory bulb manganese concentrations were also seen following gavage suggesting that dose rate is an important factor in the pharmacokinetics of oral manganese. These data will be used to refine physiologically based pharmacokinetic models, extending their utility for manganese risk assessment by including multiple dietary exposures.
Assuntos
Cloretos/farmacocinética , Dieta , Contaminação de Alimentos , Compostos de Manganês/farmacocinética , Poluentes Químicos da Água/farmacocinética , Administração Oral , Animais , Carga Corporal (Radioterapia) , Cloretos/administração & dosagem , Cloretos/toxicidade , Masculino , Compostos de Manganês/administração & dosagem , Intoxicação por Manganês/etiologia , Ratos Endogâmicos F344 , Medição de Risco , Distribuição Tecidual , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/toxicidadeRESUMO
A multi-compartment physiologically based pharmacokinetic (PBPK) model was developed to describe the behavior of Cr(III) and Cr(VI) in rats and mice following long-term oral exposure. Model compartments were included for GI lumen, oral mucosa, forestomach/stomach, small intestinal mucosa (duodenum, jejunum, ileum), blood, liver, kidney, bone, and a combined compartment for remaining tissues. Data from ex vivo Cr(VI) reduction studies were used to characterize reduction of Cr(VI) in fed rodent stomach fluid as a second-order, pH-dependent process. For model development, tissue time-course data for total chromium were collected from rats and mice exposed to Cr(VI) in drinking water for 90 days at six concentrations ranging from 0.1 to 180 mg Cr(VI)/L. These data were used to supplement the tissue time-course data collected in other studies with oral administration of Cr(III) and Cr(VI), including that from recent NTP chronic bioassays. Clear species differences were identified for chromium delivery to the target tissue (small intestines), with higher concentrations achieved in mice than in rats, consistent with small intestinal tumor formation, which was observed upon chronic exposures in mice but not in rats. Erythrocyte:plasma chromium ratios suggest that Cr(VI) entered portal circulation at drinking water concentrations equal to and greater than 60 mg/L in rodents. Species differences are described for distribution of chromium to the liver and kidney, with liver:kidney ratios higher in mice than in rats. Overall, the PBPK model provides a good description of chromium toxicokinetics, with model predictions for tissue chromium within a factor of 3 for greater than 80% of measurements evaluated. The tissue data and PBPK model predictions indicate a concentration gradient in the small intestines (duodenum > jejunum > ileum), which will be useful for assessing the tumor response gradient observed in mouse small intestines in terms of target tissue dose. The rodent PBPK model presented here, when used in conjunction with a human PBPK model for Cr(VI), should provide a more robust characterization of species differences in toxicokinetic factors for assessing the potential risks associated with low-dose exposures of Cr(VI) in human populations.
Assuntos
Cromo/farmacocinética , Modelos Biológicos , Poluentes Químicos da Água/farmacocinética , Administração Oral , Animais , Cromo/administração & dosagem , Cromo/toxicidade , Água Potável , Feminino , Humanos , Absorção Intestinal/efeitos dos fármacos , Intestino Delgado/efeitos dos fármacos , Intestino Delgado/metabolismo , Masculino , Camundongos , Ratos , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/toxicidadeRESUMO
PURPOSE: We continuously ingest barium as a general element by drinking water and foods in our daily life. Exposure to high-dose barium (>100mg/kg/day) has been shown to cause physiological impairments. Direct administration of barium to inner ears by vascular perfusion has been shown to cause physiological impairments in inner ears. However, the toxic influence of oral exposure to low-dose barium on hearing levels has not been clarified in vivo. We analyzed the toxic influence of oral exposure to low-dose barium on hearing levels and inner ears in mice. EXPERIMENTAL DESIGN: We orally administered barium at low doses of 0.14 and 1.4 mg/kg/day to wild-type ICR mice by drinking water. The doses are equivalent to and 10-fold higher than the limit level (0.7 mg/l) of WHO health-based guidelines for drinking water, respectively. After 2-week exposure, hearing levels were measured by auditory brain stem responses and inner ears were morphologically analyzed. After 2-month exposure, tissue distribution of barium was measured by inductively coupled plasma mass spectrometry. RESULTS: Low-dose barium in drinking water caused severe hearing loss in mice. Inner ears including inner and outer hair cells, stria vascularis and spiral ganglion neurons showed severe degeneration. The Barium-administered group showed significantly higher levels of barium in inner ears than those in the control group, while barium levels in bone did not show a significant difference between the two groups. Barium levels in other tissues including the cerebrum, cerebellum, heart, liver and kidney were undetectably low in both groups. CONCLUSIONS: Our results demonstrate for the first time that low-dose barium administered by drinking water specifically distributes to inner ears resulting in severe ototoxicity with degeneration of inner ears in mice.
Assuntos
Bário/administração & dosagem , Perda Auditiva/induzido quimicamente , Poluentes Químicos da Água/administração & dosagem , Estimulação Acústica , Acústica , Animais , Bário/farmacocinética , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Potenciais Evocados Auditivos do Tronco Encefálico/efeitos dos fármacos , Feminino , Rim/metabolismo , Rim/patologia , Pulmão/metabolismo , Pulmão/patologia , Camundongos , Camundongos Endogâmicos ICR , Microscopia Eletrônica de Transmissão , Miocárdio/metabolismo , Miocárdio/patologia , Neurônios/patologia , Neurônios/ultraestrutura , Espectrofotometria Atômica/métodos , Gânglio Espiral da Cóclea/patologia , Fatores de Tempo , Distribuição Tecidual/efeitos dos fármacos , Poluentes Químicos da Água/farmacocinéticaRESUMO
Ingested nitrate can be endogenously reduced to nitrite, which may form N-nitroso compounds, known potent carcinogens. However, some studies have reported no or inverse associations between dietary nitrate intake and cancer risk. These associations may be confounded by a protective effect of folate, which plays a vital role in DNA repair. We evaluated the interaction of dietary and water nitrate intake with total folate intake on breast cancer risk in the Iowa Women's Health Study. Dietary intake was assessed at study baseline. Nitrate intake from public water was assessed using a historical database on Iowa municipal water supplies. After baseline exclusions, 34,388 postmenopausal women and 2,875 incident breast cancers were included. Overall, neither dietary nor water nitrate was associated with breast cancer risk. Among those with folate intake ≥400 µg/day, breast cancer risk was significantly increased in public water users with the highest nitrate quintile (HR = 1.40, 95% CI = 1.05-1.87) and private well users (HR = 1.38, 95% CI = 1.05-1.82) compared to public water users with the lowest nitrate quintile; in contrast, there was no association among those with lower folate intake. Our findings do not support a previous report of increased risk of breast cancer among individuals with high dietary nitrate but low folate intake.
Assuntos
Neoplasias da Mama/etiologia , Dieta , Água Potável/química , Ácido Fólico/administração & dosagem , Nitratos/administração & dosagem , Idoso , Neoplasias da Mama/induzido quimicamente , Neoplasias da Mama/epidemiologia , Neoplasias da Mama/prevenção & controle , Estudos de Coortes , Dieta/efeitos adversos , Inquéritos sobre Dietas , Suplementos Nutricionais/efeitos adversos , Água Potável/efeitos adversos , Feminino , Ácido Fólico/efeitos adversos , Ácido Fólico/uso terapêutico , Aditivos Alimentares/administração & dosagem , Aditivos Alimentares/efeitos adversos , Aditivos Alimentares/análise , Humanos , Incidência , Iowa/epidemiologia , Pessoa de Meia-Idade , Nitratos/efeitos adversos , Nitratos/análise , Nitratos/toxicidade , Pós-Menopausa , Estudos Prospectivos , Sistema de Registros , Risco , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Ability of ascorbic acid (vitamin C) to attenuate oxidative damage was evaluated in liver and brain tissues of Oreochromis niloticus (O. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (CPF). O. niloticus was exposed to sublethal concentrations of CPF at 12 µg/L (CPF1) and 24 µg/L (CPF2) for 96 h. The fish of vitamin C (Vit C) and CPF2 + Vit C groups were fed with Vit C supplemented diet (200 mg Vit C/100 g feed). A significant increase in thiobarbituric acid-reactive substances (TBARS) level (P < 0.05) was observed in brain of CPF-exposed fish although liver TBARS level was not changed compared to control group. This result showed that lipid peroxidation (LPO) was elevated in brain of fish exposed to CPF. Glutathione peroxidase (GSH-Px) activity in liver and brain tissues was significantly elevated (P < 0.05) by exposure to CPF1 and CPF2. Catalase (CAT) activity was significantly increased (P < 0.05) in liver but decreased in brain of treated fish by CPF2 concentration. Superoxide dismutase (SOD) activity was decreased in liver, but increased in brain by exposure to CPF1 and CPF2 concentrations. Levels of TBARS were increased in brain of CPF-treated animals, but tended to decrease by the effect of Vit C. Vit C treatment for CPF-intoxicated animals normalized the otherwise raised activities of GSH-Px, CAT, and SOD within normal limits. The results clearly indicate that exposure to CPF caused a dose-dependent increase in oxidative stress brain and to a lesser extend in liver of fish and the ability of Vit C to attenuate CPF-induced oxidative damage.
Assuntos
Ácido Ascórbico/farmacologia , Clorpirifos/toxicidade , Ciclídeos/metabolismo , Inseticidas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Animais , Ácido Ascórbico/administração & dosagem , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Catalase/metabolismo , Clorpirifos/administração & dosagem , Relação Dose-Resposta a Droga , Proteínas de Peixes/metabolismo , Glutationa Peroxidase/metabolismo , Inseticidas/administração & dosagem , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/toxicidadeRESUMO
Experiments were performed in the laboratory to determine if excess levels of Co used as dietary supplement (0.0, 0.05, 0.10 and 1.0%) to enhance growth of the fish Cyprinus carpio was safe for aquatic organisms. Lethal concentrations of Co for tadpole of toad Bufo melanostictus (96 h LC(50), 17.2 mg/L), oligochaet worm Branchiura sowerbyi (96 h LC(50), 179 mg/L) and crustacean zooplankton Diaptomus forbesi (96 h LC(50), 1.5 mg/L) were compared with the concentration of Co in the medium leached from the unused diets and faeces. The results indicated that the Co leached from diet containing 1.0% Co was ecotoxicologically unsafe for crustacean zooplankton.
Assuntos
Cobalto/toxicidade , Dieta/efeitos adversos , Suplementos Nutricionais/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Bufonidae , Carpas , Cobalto/administração & dosagem , Diatomáceas , Relação Dose-Resposta a Droga , Ecotoxicologia , Oligoquetos , Poluentes Químicos da Água/administração & dosagemRESUMO
The acute and sub-chronic toxicities of cyanobacterial extract from Taihu Lake (PR China) on mouse (Mus musculus) were investigated in this study via intraperitoneal (i.p.) injection. Increases in liver/body weight ratios and pathological changes in mouse liver showed adverse effects at the organ level. Images from transmission electron microscopy (TEM) indicated that abnormal membrane structure occurred and that the organelles were damaged severely in the cells of liver and testis. The high dose group received i.p. injection of 12 mg lyophilized algae cells/kg body weight. Malondialdehyde (MDA) levels increased significantly in the livers of this group, along with a significant decrease in catalase (CAT) activity. These results revealed the existence of obvious oxidative stress. Comet assay results also suggested a dose-dependent relationship between DNA damage in hepatocytes/testicular cells and the amount of bloom extract administered to the mice. There was a significant increase in DNA damage compared to the control group and the genotoxicity of the cyanobacterial bloom to testicular cells was higher than in hepatocytes.
Assuntos
Toxinas Bacterianas/toxicidade , Cianobactérias/patogenicidade , Água Doce/microbiologia , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Microbiologia da Água , Poluentes Químicos da Água/toxicidade , Animais , Toxinas Bacterianas/administração & dosagem , Peso Corporal/efeitos dos fármacos , Catalase/metabolismo , China , Toxinas de Cianobactérias , Relação Dose-Resposta a Droga , Monitoramento Ambiental , Água Doce/química , Injeções Intraperitoneais , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Toxinas Marinhas/administração & dosagem , Camundongos , Microcistinas/administração & dosagem , Mutagênicos/administração & dosagem , Mutagênicos/toxicidade , Superóxido Dismutase/metabolismo , Poluentes Químicos da Água/administração & dosagemRESUMO
Suppression subtractive hybridization (SSH) cDNA library construction and characterization was used to identify differentially regulated transcripts from oil exposure in liver of male Atlantic herring (Clupea harengus) fed a diet containing 900 mg crude oil/kg for 2 mo. In total, 439 expressed sequence tags (EST) were sequenced, 223 from the forward subtracted library (enriched for genes putatively upregulated by oil exposure) and 216 from the reverse subtracted library (enriched for genes putatively downregulated by oil exposure). Follow-up reverse-transcription (RT) quantitative polymerase chain reaction (qPCR) analyses of gene transcription were conducted on additional herring exposed to food containing 9 (low), 90 (medium), and 900 (high) mg crude oil/kg feed for 2 mo. Chronic exposure of Atlantic herring to an oil-contaminated diet mediated upregulation of transcripts encoding antifreeze proteins, proteins in the classical complement pathway (innate immunity), and iron-metabolism proteins. Gene ontology (GO) analysis showed that "cellular response to stress," "regulation to biological quality," "response to abiotic stimuli," and "temperature homeostasis" were the most affected go at the biological processes level, and "carbohydrate binding," "water binding," and "ion binding" at the molecular function level. Of the genes examined with RT-qPCR, CYP1A, antifreeze protein, retinol binding protein 1, deleted in malignant brain tumor 1, and ovary-specific C1q-like factor demonstrated a significant upregulation. Myeloid protein 1, microfibrillar-associated protein 4, WAP65, and pentraxin were downregulated in liver of fish from the high exposure group. In conclusion, this study suggests that 2 mo of oil exposure affected genes encoding proteins involved in temperature homeostasis and possible membrane stability in addition to immune-responsive proteins in Atlantic herring.
Assuntos
Copépodes , Proteínas de Peixes/genética , Peixes/genética , Petróleo/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Dieta/estatística & dados numéricos , Relação Dose-Resposta a Droga , Monitoramento Ambiental , Feminino , Proteínas de Peixes/metabolismo , Peixes/metabolismo , Expressão Gênica/efeitos dos fármacos , Biblioteca Gênica , Fígado/metabolismo , Masculino , Poluentes Químicos da Água/administração & dosagemAssuntos
Serviços de Saúde da Criança/organização & administração , Desastres , Exposição Ambiental/estatística & dados numéricos , Petróleo , Poluentes Químicos da Água/administração & dosagem , Oceano Atlântico , Criança , Proteção da Criança/estatística & dados numéricos , Planejamento em Desastres/organização & administração , Humanos , Enfermagem Pediátrica/organização & administraçãoRESUMO
Common carp, Cyprinus carpio, was exposed to 1% and 5% dietary heavy oil for 2 weeks. Higher polyaromatic hydrocarbons (PAHs) accumulation induced higher hepatic EROD activity in exposure groups compared with the control group. Significant histological alternations were observed in the liver such as nuclear and cellular hypertrophy, accumulation of eosinophilic granules in cytoplasm, nuclear degeneration and fibrillar inclusion in cytoplasm. Severe damage of the kidney was indicated by nuclear and cellular degeneration, and ultimately necrosis in the tubular epithelium cells. In addition, dilated glomerulus with hemorrhage was found in the renal capsule. The mean assessment value (MAV) and degree of tissue changes (DTC), as semi-quantitative analyses, were significantly increased with concentration and duration of exposure. Likewise, frequencies of pathological lesions in both liver and kidney were also increased with concentration and duration of exposure.
Assuntos
Carpas/metabolismo , Citocromo P-450 CYP1A1/metabolismo , Petróleo/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Biomarcadores/metabolismo , Dieta , Monitoramento Ambiental , Água Doce/química , Rim/efeitos dos fármacos , Rim/metabolismo , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Poluentes Químicos da Água/administração & dosagemRESUMO
Female catfish, Clarias gariepinus, were collected from the Nile River at Assiut region, were divided into 7 groups. The first group was left as control, and the second was treated with mercuric chloride (MC) for 3 weeks following by normal water for 1 week. The third, fourth and fifth groups were provided by MC (150 µg/ l of water). This treatment was continued for 3 weeks. Then, the fish were received CN instead of MC, for 1 week, with 15 and 25 mg CN/100 g wet food. The fifth fish group received diet supplemented with vit E (α-tocopherol) (100 mg/kg wet diet), for 1 week, instead of MC treatment. Vitamin E was used as standard antioxidant drug. Following 3 weeks of normal ambient water, the sixth and seventh aquaria received only CN for 1 week, with 15 and 25 mg CN respectively/100 g wet food, respectively. At the end of the experiment, Samples of liver, kidneys (posterior part), gills (right gills) and ovary were excised. The measurement included the oxidative stress parameters: carbonyl protein and total peroxide and the antioxidant enzyme activities superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) in all selected organs. MC treatment induced harmful effect in fish, probably due to its enhancing effect on reactive oxygen species (ROS) production in fish organs especially the respiratory and osmoregulatory organs namely gills. The result suggests that this gill damage may exert hypoxic case, anoxia for different organs and some Cu excretion resulting in a magnification of ROS overproduction. Also, the observed oxidative stress in ovary tissue of MC-treated fish may affect fish fertility. The addition of CN in fish diets could protect the fish C. gariepinus against MC-induced oxidative damage showing recovery of fish organs. It could suggest that the detoxifying mechanism of action of CN is mainly due to its scavenging activity of free radicals rather than tissue healing.
Assuntos
Peixes-Gato , Cobre/farmacologia , Doenças dos Peixes/induzido quimicamente , Cloreto de Mercúrio/toxicidade , Niacina/farmacologia , Níquel/farmacologia , Animais , Antioxidantes/farmacologia , Biomarcadores , Quelantes/administração & dosagem , Quelantes/farmacologia , Cobre/administração & dosagem , Relação Dose-Resposta a Droga , Feminino , Niacina/administração & dosagem , Níquel/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Vitamina E/farmacologia , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/toxicidadeRESUMO
Populations of villages of eastern India and Bangladesh and many other parts of the world are exposed to arsenic mainly through drinking water. Due to non-availability of safe drinking water they are compelled to depend on arsenic-contaminated water. Generally, poverty level is high in those areas and situation is compounded by the lack of proper nutrition. The hypothesis that the deleterious health effects of arsenic can be prevented by modification of dietary factors with the availability of an affordable and indigenous functional food jaggery (sugarcane juice) has been tested in the present study. Jaggery contains polyphenols, vitamin C, carotene and other biologically active components. Arsenic as sodium-m-arsenite at low (0.05 ppm) and high (5 ppm) doses was orally administered to Swiss male albino mice, alone and in combination with jaggery feeding (250 mg/mice), consecutively for 180 days. The serum levels of total antioxidant, glutathione peroxidase and glutathione reductase were substantially reduced in arsenic-exposed groups, while supplementation of jaggery enhanced their levels in combined treatment groups. The serum levels of interleukin-1beta, interleukin-6 and TNF-alpha were significantly increased in arsenic-exposed groups, while in the arsenic-exposed and jaggery supplemented groups their levels were normal. The comet assay in bone marrow cells showed the genotoxic effects of arsenic, whereas combination with jaggery feeding lessened the DNA damage. Histopathologically, the lung of arsenic-exposed mice showed the necrosis and degenerative changes in bronchiolar epithelium with emphysema and thickening of alveolar septa which was effectively antagonized by jaggery feeding. These results demonstrate that jaggery, a natural functional food, effectively antagonizes many of the adverse effects of arsenic.
Assuntos
Arsênio/toxicidade , Mutagênicos/toxicidade , Extratos Vegetais/uso terapêutico , Poluentes Químicos da Água/toxicidade , Administração Oral , Animais , Antioxidantes/metabolismo , Arsênio/administração & dosagem , Bangladesh , Células da Medula Óssea/metabolismo , Ensaio Cometa , Dano ao DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Glutationa Peroxidase/sangue , Glutationa Peroxidase/efeitos dos fármacos , Glutationa Redutase/sangue , Glutationa Redutase/efeitos dos fármacos , Índia , Pulmão/efeitos dos fármacos , Pulmão/patologia , Masculino , Camundongos , Testes de Mutagenicidade , Mutagênicos/administração & dosagem , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Áreas de Pobreza , Poluentes Químicos da Água/administração & dosagemRESUMO
Halogenated acetic acids (HAAs) produced by chlorine disinfection of municipal drinking water represent a potentially important class of environmental contaminants. Little is known, however, about their potential to adversely impact fish and other aquatic life. In this study we examined the kinetics and effects of dichloroacetic acid (DCA) in rainbow trout. Branchial uptake was measured in fish confined to respirometer-metabolism chambers. Branchial uptake efficiency was <5%, suggesting passive diffusion through aqueous channels in the gill epithelium. DCA concentrations in tissues following prolonged (72, 168, or 336 h) waterborne exposures were expressed as tissue:plasma concentration ratios. Concentration ratios for the kidney and muscle at 168 and 336 h were consistent with the suggestion that DCA distributes primarily to tissue water. Reduced concentration ratios for the liver, particularly at 72 h, indicated that DCA was highly metabolized by this tissue. Routes and rates of elimination were characterized by injecting chambered animals with a high (5.0mg/kg) or low (50 microg/kg) bolus dose. DCA was rapidly cleared by naïve animals resulting in elimination half-lives (t(1/2)) of less than 4h. Waterborne pre-treatment of fish with DCA increased the persistence of a subsequently injected dose. In high dose animals, pre-treatment caused a 4-fold decrease in whole-body clearance (CL(B)) and corresponding increases in the area under the plasma concentration-time curve (extrapolated to infinity; AUC(0-->infinity)) and t(1/2). Qualitatively similar results were obtained in low dose fish, although the magnitude of the pre-treatment effect ( approximately 2.5-fold) was reduced. Renal and branchial clearance contributed little (combined, <3% of CL(B)) to the elimination of DCA. Biliary elimination of DCA was also negligible. The steady-state volume of distribution (V(SS)) did not vary among treatment groups and was consistent with results of the tissue distribution study. DCA had no apparent effects on respiratory physiology or acid-base balance; however, the concentration of blood lactate declined progressively during continuous waterborne exposures. A transient effect on blood lactate was also observed in bolus injection experiments. The results of this study suggest that clearance of DCA is due almost entirely to metabolism. The pathway responsible for this activity exhibits characteristics in common with those of mammalian glutathione S-transferase zeta (GSTzeta), including non-linear kinetics and apparent suicide inactivation by DCA. Observed effects on blood lactate are probably due to the inhibition of pyruvate dehydrogenase kinase in aerobic tissues and may require the participation of a monocarboxylase transport protein to move DCA across cell membranes.
Assuntos
Ácido Dicloroacético/farmacocinética , Ácido Dicloroacético/toxicidade , Oncorhynchus mykiss/metabolismo , Poluentes Químicos da Água/farmacocinética , Poluentes Químicos da Água/toxicidade , Animais , Biotransformação , Ácido Dicloroacético/administração & dosagem , Brânquias/metabolismo , Injeções , Taxa de Depuração Metabólica , Distribuição Tecidual , Testes de Toxicidade , Poluentes Químicos da Água/administração & dosagemRESUMO
In this age of modern biology, aquatic toxicological research has provided potential tools for ecotoxicologic investigations. Heavy metals primarily affect protein structures and induce a stress in the organisms. The present investigation was carried out to assess the effect of nickel chloride on the selected organs of the freshwater fish Cirrhinus mrigala and how CaNa(2) EDTA counters its effects as an antidote. Toxicity experiments were conducted for different exposure periods and also in certain tissues namely gill, liver, kidney and muscle. The total protein content, reduced glutathione, glutathione peroxidase and lipid peroxidation were found to be decreased in the nickel chloride treated tissues and the treatment with CaNa(2) EDTA+nickel chloride returned to near normal levels. Histopathological observations also revealed that after the administration of nickel chloride+CaNa(2) EDTA the chelator induced reduction in nickel toxicity. It has also contributed towards reduction in the pathological damage, thus enabling the organs to attain their near normal histological appearance. The present study shown that CaNa(2) EDTA is an effective chelating agent for the removal of nickel and it has proved efficient in restoring both the biochemical variables and pathological features immediately after a sub lethal exposure of nickel chloride in fish.
Assuntos
Quelantes/farmacologia , Cyprinidae/metabolismo , Ácido Edético/farmacologia , Glutationa Peroxidase/metabolismo , Glutationa/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Níquel/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Quelantes/uso terapêutico , Terapia por Quelação , Cyprinidae/anatomia & histologia , Avaliação Pré-Clínica de Medicamentos/veterinária , Ácido Edético/uso terapêutico , Doenças dos Peixes/induzido quimicamente , Doenças dos Peixes/metabolismo , Doenças dos Peixes/patologia , Brânquias/química , Brânquias/patologia , Rim/química , Rim/patologia , Fígado/química , Fígado/patologia , Músculo Esquelético/química , Músculo Esquelético/patologia , Níquel/administração & dosagem , Oxirredução , Proteínas/análise , Poluentes Químicos da Água/administração & dosagemRESUMO
The release of produced water (PW), a by-product of offshore oil production, has increased in Norwegian waters in recent years. Alkylphenols (AP), a major component of PW, have been shown to have endocrine disrupting effects on several fish species. In the present study, four groups of female Atlantic cod (Gadus morhua) were orally exposed for 20 weeks to two different concentrations of a mixture of C4-C7 APs, PW or 17beta-estradiol. The transcriptional responses in the liver of Atlantic female cod were studied using a custom-made cDNA microarray. The largest transcriptional effects were seen in cod exposed to the lowest dose of APs. Several biological processes such as glycolysis, apoptosis and the general stress response were affected by exposure to APs. In addition, genes coding for the detoxification enzymes CYP1A and sulfotransferase 2 were up-regulated in the low exposure group. Significant reduction in gonadosomatic index (GSI) and the concentration of plasma vitellogenin were seen in both AP and 17beta-estradiol exposed cod. Exposure to PW had little effect on GSI and the regulation of stress responsive genes. The findings indicate that chronic exposure to low levels of APs may cause a stress response and delayed maturation in female cod.
Assuntos
Disruptores Endócrinos/toxicidade , Gadus morhua/genética , Petróleo/toxicidade , Fenóis/toxicidade , Estresse Fisiológico/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Administração Oral , Animais , Relação Dose-Resposta a Droga , Disruptores Endócrinos/administração & dosagem , Estradiol/toxicidade , Feminino , Gadus morhua/metabolismo , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica/efeitos dos fármacos , Gônadas/efeitos dos fármacos , Mar do Norte , Noruega , Análise de Sequência com Séries de Oligonucleotídeos , Fenóis/administração & dosagem , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Água do Mar , Estresse Fisiológico/genética , Ativação Transcricional/efeitos dos fármacos , Vitelogeninas/sangue , Poluentes Químicos da Água/administração & dosagemRESUMO
Quebracho extracts are used in tannery due to their high concentration of phenolics. The Mexican tannery industry uses around 450 kg/m(3) of which, 150 kg/m(3) remains in wastewaters and are discharged in drain pipe systems or rivers. The quebracho phenolics recovered from tannery wastewater (QPTW) was characterized by HPLC. The antimutagenic and antioxidant activities as well as the microbiological quality were evaluated. Total phenolic content of QPTW was 621mg catechin equivalent/g sample. Gallic and protocatechuic acids were the major components characterized by HPLC. QPTW showed an inhibition range on aflatoxin B(1) mutagenicity from 16 to 60% and was dose-dependent. Antioxidant activity (defined as beta-carotene bleaching) of QPTW (64.4%) at a dose of 12.3mg/mL was similar to that of BHT (68.7%) at a dose of 0.33 mg/mL, but lower than Trolox (90.8% at a dose of 2.5mg/mL); meanwhile antiradical activity (measured as reduction of DPPH) (60.8%) was higher than that of BHT (50.8%) and Trolox (34.2%). Quebracho residues were demonstrated to be an outstanding source of phenolic acids and for research and industrial uses.