RESUMO
Understanding the biogeochemical U redox processes is crucial for controlling U mobility and toxicity under conditions relevant to deep geological repositories (DGRs). In this study, we examined the microbial reduction of aqueous hexavalent uranium U(VI) [U(VI)aq] by indigenous bacteria in U-contaminated groundwater. Three indigenous bacteria obtained from granitic groundwater at depths of 44-60 m (S1), 92-116 m (S2), and 234-244 m (S3) were used in U(VI)aq bioreduction experiments. The concentration of U(VI)aq was monitored to evaluate its removal efficiency for 24 weeks under anaerobic conditions with the addition of 20 mM sodium acetate. During the anaerobic reaction, U(VI)aq was precipitated in the form of U(IV)-silicate with a particle size >100 nm. The final U(VI)aq removal efficiencies were 37.7%, 43.1%, and 57.8% in S1, S2, and S3 sample, respectively. Incomplete U(VI)aq removal was attributed to the presence of a thermodynamically stable calcium uranyl carbonate complex in the U-contaminated groundwater. High-throughput 16S rRNA gene sequencing analysis revealed the differences in indigenous bacterial communities in response to the depth, which affected to the U(VI)aq removal efficiency. Pseudomonas peli was found to be a common bacterium related to U(VI)aq bioreduction in S1 and S2 samples, while two SRB species, Thermodesulfovibrio yellowstonii and Desulfatirhabdium butyrativorans, played key roles in the bioreduction of U(VI)aq in S3 sample. These results indicate that remediation of U(VI)aq is possible by stimulating the activity of indigenous bacteria in the DGR environment.
Assuntos
Biodegradação Ambiental , Urânio , Bactérias/genética , Água Subterrânea/microbiologia , Oxirredução , RNA Ribossômico 16S/genética , Urânio/análise , Urânio/metabolismo , Poluentes Radioativos da Água/análise , Poluentes Radioativos da Água/metabolismoRESUMO
Biosorption-based technologies have been proposed for the removal of radionuclides from radioactive liquid waste containing organic compounds. Nevertheless, pytoremediation potential of uranium (U) by nonliving aquatic macrophytes Lemna sp. and Pistia stratiotes has not been previously addressed. In this study, uranium biosorption capacity by Pistia stratiotes and Lemna sp. was evaluated by equilibrium and kinetics experiments. The biomasses were added to synthetic and real waste solutions. The assays were tested in polypropylene vials containing 10â¯mL of uranium nitrate solution and 0.20â¯g of biomass. Solutions ranging from 0.25 to 84.03â¯mmolâ¯l-1 were employed for the assessment of uranium concentration in each macrophyte. The equilibrium time was 1â¯h for both macrophytes. Lemna sp. achieved the highest sorption capacity with the use of the synthetic solution, which was 0.68â¯mmolâ¯g-1 for the macrophyte. Since Lemna sp. exhibit a much higher adsorption capacity, only this biomass was exposed to the actual waste solution, being able to adsorb 9.24â¯×â¯10-3â¯mmolâ¯g-1 U (total). The results show that these materials are potentially applicable to the treatment of liquid radioactive waste.
Assuntos
Araceae/metabolismo , Biodegradação Ambiental , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , AdsorçãoRESUMO
This study evaluated U(VI) biosorption properties by the resistant bacterium, Bacillus amyloliquefaciens, which was isolated from the soils with residual radionuclides. The effect of biosorption factors (uptake time, pH, ionic concentration, biosorbent dosage and temperature) on U(VI) removal was determined by batch experiments. The uptake processes were characterized by using SEM, FTIR, and XPS. The experimental data of U(VI) biosorption were fitted by the pseudo-second-order. The maximum uptake capacity was 179.5â¯mg/g at pH 6.0 by Langmuir model. The thermodynamic results: ΔGо, ΔHо and ΔSо for uptake processes were calculated as -6.359â¯kJ/mol, 14.20â¯kJ/mol and 67.19â¯J/mol/K, respectively. The results showed that the biosorption of Bacillus amyloliquefaciens will be an ideal method to remove radionuclides.
Assuntos
Bacillus amyloliquefaciens/metabolismo , Biodegradação Ambiental , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Adsorção , Cinética , TermodinâmicaRESUMO
Aspergillus niger (A. niger) and Syngonium podophyllum (S. podophyllum) have been used for wastewater treatment, and have exhibited a promising application in recent years. To determine the effects of A. niger on uranium enrichment and uranium stress antagonism of S. podophyllum, the S. podophyllum-A. niger combined system was established, and hydroponic remediation experiments were carried out with uranium-containing wastewater. The results revealed that the bioaugmentation of A. niger could increase the biomass of S. podophyllum by 5-7%, reverse the process of U(VI) reduction induced by S. podophyllum, and increase the bioconcentration factor (BCF) and translocation factor (TF) of S. podophyllum to uranium by 35-41 and 0.01-0.06, respectively, thereby improving the reduction of uranium in wastewater. Moreover, A. niger could promote the cell wall immobilization and the subcellular compartmentalization of uranium in the root of S. podophyllum, reduce the phytotoxicity of uranium entering root cells, and inhibit the calcium efflux from root cells, thereby withdrawing the stress of uranium on S. podophyllum.
Assuntos
Araceae/crescimento & desenvolvimento , Aspergillus niger/metabolismo , Urânio/análise , Águas Residuárias/química , Poluentes Radioativos da Água/análise , Purificação da Água/métodos , Araceae/metabolismo , Biodegradação Ambiental , Biomassa , Hidroponia , Minerais , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismoRESUMO
The bacterial community of an anaerobic granular sludge associated with uranium depletion was investigated following its exposure to uranium under different initial pH conditions (pH 4.5, 5.5, and 6.5). The highest uranium removal efficiency (98.1%) was obtained for the sample with an initial pH of 6.5, which also supported the highest bacterial community richness and diversity. Venn diagrams visualized the decrease in the number of genera present in both the inoculum and the uranium-exposed biomass as the initial pH decreased from 6.5 to 4.5. Compared with the inoculum, a significant increase in the abundances of the phyla Chloroflexi and Proteobacteria was observed following uranium exposure. At initial pH conditions of 6.5 to 4.5, the proportions of the taxa Anaerolineaceae, Chryseobacterium, Acinetobacter, Pseudomonas, and Sulfurovum increased significantly, likely contributing to the observed uranium removal. Uranium exposure induced a greater level of dynamic diversification of bacterial abundances than did the initial pH difference.
Assuntos
Reatores Biológicos/microbiologia , Urânio/análise , Eliminação de Resíduos Líquidos/métodos , Poluentes Radioativos da Água/análise , Anaerobiose , Bactérias , Bactérias Anaeróbias , Biodegradação Ambiental , Biomassa , Chloroflexi , Concentração de Íons de Hidrogênio , Proteobactérias , Esgotos , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismoRESUMO
Uranium (U) toxicity patterns for fish have been mainly determined under laboratory-controlled waterborne exposure conditions. Because fish can take up metals from water and diet under in situ exposure conditions, a waterborne U exposure experiment (20 µg L-1 , 20 d) was conducted in the laboratory to investigate transfer efficiency and target organ distribution in zebrafish Danio rerio compared with combined waterborne exposure (20 µg L-1 ) and diet-borne exposure (10.7 µg g-1 ). 233 Uranium was used as a specific U isotope tracer for diet-borne exposure. Bioaccumulation was examined in the gills, liver, kidneys, intestine, and gonads of D. rerio. Concentrations in the organs after waterborne exposure were approximately 500 ng g-1 fresh weight, except in the intestine (> 10 µg g-1 fresh wt) and the kidneys (200 ng g-1 fresh wt). No significant difference was observed between waterborne and diet-borne conditions. Trophic U transfer in organs was found but at a low level (< 10 ng g-1 fresh wt). Surprisingly, the intestine appeared to be the main target organ after both tested exposure modalities. The gonads (57% at 20 d) and the liver (41% at 20 d) showed the highest accumulated relative U burdens. Environ Toxicol Chem 2019;38:90-98. © 2018 SETAC.
Assuntos
Dieta , Exposição Ambiental , Especificidade de Órgãos , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Peixe-Zebra/metabolismo , AnimaisRESUMO
This study analyzed the ability of Nymphaea tetragona Georgi (N. tetragona) to accumulate water-borne uranium and any effects this could exert on this plant species. In accumulation experiments, N. tetragona was exposed (21â¯d) to different concentrations of uranium (0-55â¯mgâ¯L-1) and the content of uranium was determined in water and plant tissues (leaves, submerged position and plant) to determine the translocation factor (TF) and bioconcentration factor (BCF). The content of uranium in the plant and plant tissues showed concentration-dependent uptake, leaves were the predominant tissue for uranium accumulation, and TF and BCF values were both affected by the concentration of uranium in the water. In this research, the uranium content and BCF value in the leaves of N. tetragona were upto 3446⯱â¯155â¯mgâ¯kg-1 and 73⯱â¯3, respectively. In physiological experiments, uranium treatment boosted the activity of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in the leaves, and increasing uranium concentrations aggravated damage to the cell membrane system. Uranium contamination significantly inhibited the content of soluble protein, as well as chlorophyll-a, chlorophyll-b and carotene in the leaves, indicating the structure and function of chloroplast were destroyed, reducing the photosynthetic performance of plants. These results indicate that the macrophyte N. tetragona can accumulate uranium while showing a stress response via metabolic mechanisms under uranium exposure, and it may be a suitable bioremediation candidate for aquatic marine contamination.
Assuntos
Nymphaea/toxicidade , Urânio/toxicidade , Poluentes Radioativos da Água/toxicidade , Biodegradação Ambiental , Monitoramento Ambiental , Nymphaea/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismoRESUMO
Overexpression of the enzyme phosphatase (PhoN/PhoK) in the radiation-resistant bacterium Deinococcus radiodurans could be an efficient strategy for uranium remediation. However, the presence of other metals in nuclear wastes often interferes with uranium bioprecipitation. In our study, the uranium-precipitating ability of the PhoN-expressing D. radiodurans strain (Deino-phoN) significantly decreased by 45.4% in 13â¯h in the presence of chromium (VI); however, it was partially recovered after supplementation with chromium (III). Therefore, the reduction of chromium (VI) to chromium (III) was obtained by the co-expression of the YieF protein and PhoN in D. radiodurans (Deino-phoN-yieF). As a result, an increase in the chromium (VI) reduction (25.1%) rate was observed in 24â¯h. Furthermore, uranium precipitation also increased by 28.0%. For the decontamination of groundwater, we immobilized Deino-phoN-yieF cells using Polyvinyl alcohol (PVA)-sodium alginate (SA) beads, followed by incubation in a bioreactor. Approximately 99% of chromium (VI) and uranium (VI) was removed after 4 continuous cycles operated for a period of over 20 days at room temperature (25⯰C). Therefore, Deino-phoN-yieF could be used as a potential biological agent for mixed radioactive nuclear waste remediation.
Assuntos
Biodegradação Ambiental , Cromo/metabolismo , Deinococcus/metabolismo , Urânio/isolamento & purificação , Proteínas de Bactérias/metabolismo , Precipitação Química , Oxirredutases/metabolismo , Monoéster Fosfórico Hidrolases/metabolismo , Resíduos Radioativos , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismoRESUMO
Remediation of former uranium mining sites represents one of the biggest challenges worldwide that have to be solved in this century. During the last years, the search of alternative strategies involving environmentally sustainable treatments has started. Bioremediation, the use of microorganisms to clean up polluted sites in the environment, is considered one the best alternative. By means of culture-dependent methods, we isolated an indigenous yeast strain, KS5 (Rhodosporidium toruloides), directly from the flooding water of a former uranium mining site and investigated its interactions with uranium. Our results highlight distinct adaptive mechanisms towards high uranium concentrations on the one hand, and complex interaction mechanisms on the other. The cells of the strain KS5 exhibit high a uranium tolerance, being able to grow at 6 mM, and also a high ability to accumulate this radionuclide (350 mg uranium/g dry biomass, 48 h). The removal of uranium by KS5 displays a temperature- and cell viability-dependent process, indicating that metabolic activity could be involved. By STEM (scanning transmission electron microscopy) investigations, we observed that uranium was removed by two mechanisms, active bioaccumulation and inactive biosorption. This study highlights the potential of KS5 as a representative of indigenous species within the flooding water of a former uranium mine, which may play a key role in bioremediation of uranium contaminated sites.
Assuntos
Biodegradação Ambiental , Urânio/metabolismo , Ustilaginales/metabolismo , Poluentes Radioativos da Água/metabolismo , Inundações , Mineração , Tolerância a Radiação , Temperatura , Ustilaginales/crescimento & desenvolvimento , Ustilaginales/isolamento & purificação , Ustilaginales/ultraestrutura , Água/metabolismoRESUMO
Paecilomyces catenlannulatus (P. catenlannulatus) as a genus of entomogenous fungus presented a variety of surface reactive groups by batch characterizations. The detoxification of U(VI) by P. catenlannulatus was investigated under different water chemistry (pH, incubation time, foreign anions and U(VI) concentration) by batch techniques. Approximately 75% of U(VI) was reduced to U(IV) (i.e., U(IV)O2(s)) by P. catenlannulatus at pH 5.5 and 7 days under glovebox conditions, therefore the formation of precipitates decreased the toxicity of U(VI) for P. catenlannulatus. In addition, phosphate facilitate the U(VI) reduction, whereas carbonate and sulfate inhibited the U(VI) reduction. The activities of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) level were stimulated exposure to 1-30â¯mg/L U(VI), indicating that CAT, SOD and GSH were antagonized for the oxidant stress derived from U(VI) at low concentrations. According to XPS and XANES analysis, the occurrence of U(IV) revealed the reduction of adsorbed U(VI) to U(IV) by P. catenlannulatus. The results of EXAFS analysis indicated that the fitting of U-O and U-U shell for U-loaded P. catenlannulatus was similar to that of U(IV)O2(s)). The formation of U-bearing precipitates decreased the toxicity of U(VI) for P. catenlannulatus. These findings indicated that P. catenlannulatus is capable to detoxify U(VI) by extracellar/intracellar defense systems. Therefore, P. catenlannulatus can be utilized as a promising bioadsorbents for remediation of uranium-contaminated wastewater in environmental cleanup.
Assuntos
Biodegradação Ambiental , Paecilomyces/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Espectroscopia por Absorção de Raios XRESUMO
A large fraction (47%) of the world's uranium is mined by a technique called "In Situ Recovery" (ISR). This mining technique involves the injection of a leaching fluid (acidic or alkaline) into a uranium-bearing aquifer and the pumping of the resulting solution through cation exchange columns for the recovery of dissolved uranium. The present study reports the in-depth alterations brought to autochthonous microbial communities during acidic ISR activities. Water samples were collected from a uranium roll-front deposit that is part of an ISR mine in operation (Tortkuduk, Kazakhstan). Water samples were obtained at a depth of ca 500â¯m below ground level from several zones of the Uyuk aquifer following the natural redox zonation inherited from the roll front deposit, including the native mineralized orebody and both upstream and downstream adjacent locations. Samples were collected equally from both the entrance and the exit of the uranium concentration plant. Next-generation sequencing data showed that the redox gradient shaped the community structures, within the anaerobic, reduced, and oligotrophic habitats of the native aquifer zones. Acid injection induced drastic changes in the structures of these communities, with a large decrease in both cell numbers and diversity. Communities present in the acidified (pH valuesâ¯<â¯2) mining areas exhibited similarities to those present in acid mine drainage, with the dominance of Sulfobacillus sp., Leptospirillum sp. and Acidithiobacillus sp., as well as the archaean Ferroplasma sp. Communities located up- and downstream of the mineralized zone under ISR and affected by acidic fluids were blended with additional facultative anaerobic and acidophilic microorganisms. These mixed biomes may be suitable communities for the natural attenuation of ISR mining-affected subsurface through the reduction of metals and sulfate. Assessing the effect of acidification on the microbial community is critical to evaluating the potential for natural attenuation or active bioremediation strategies.
Assuntos
Água Subterrânea/microbiologia , Mineração , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Ácidos , Bactérias , Água Subterrânea/químicaRESUMO
This study investigated the interaction of inorganic aqueous Eu(III), Pb(II), and U(VI) with Paramecium sp., a representative single-celled protozoan that lives in freshwater. Living and prekilled Paramecium cells were tested. The prekilled cells were killed with a fixative. After 24â¯h exposure of the cells to inorganic aqueous solutions containing Eu(III) or U(VI), analyses by microparticle-induced X-ray emission with a focused beam (<1⯵m) did not detect Eu and U in the living cells, whereas Eu and U were detected in the prekilled cells. Size exclusion chromatography coupled with on-line ultraviolet-visible detection and elemental detection by inductively coupled plasma mass spectrometry of the aqueous phases collected after the living cell experiments revealed that a fraction of the Eu, Pb, and U in the aqueous phase bound to a large (ca. 250â¯kDa) Paramecium biomolecule and formed a metal-organic complex. The characteristics of the biomolecule were consistent with those of the soluble glycoproteins covering the surfaces of Paramecium cells. These results show that Paramecium cells transform inorganic aqueous Eu, Pb, and U to organic complexes. This paper discusses the relation between this novel complexation and the sorption of these heavy elements on Paramecium cells.
Assuntos
Európio/metabolismo , Glicoproteínas/metabolismo , Chumbo/metabolismo , Paramecium/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Adsorção , Cromatografia em Gel , Complexos de Coordenação/análise , Água Doce/química , Concentração de Íons de HidrogênioRESUMO
The influences of N species and transpiration on the Fe plaque (IP) formation and related aquatic U rhizofiltration had not revealed yet, especially when these factors were co-existed. It was evaluated in a mesocosm experiment in the condition of respective ammonium (NH4+)/nitrate (NO3-) cultivation of Phragmites australis Trin ex Steud. coupled with different transpiration rates (TRs). The results suggested that the enhanced transpiration of P. australis improved the aquatic U rhizofiltration in both NO3- and NH4+ rich milieus. However, the NO3- dependent oxidizing milieu restricted aquatic U uptake by the root of P. australis (up to 47.6 ± 1.8 mg kg-1 under high TR) via IP assisted rhizofiltration. The high aquatic U availability and limited IP formation in NO3- rich milieu benefited the U retention within root tissue. On the contrary, the aquatic U rhizofiltration (up to 62.1 ± 1.0 mg kg-1 under high TR) was enhanced under NH4+ dependent reductive milieu. It was mainly contributed by U retention within IP. The area related U accumulation in different N species cultured roots was enhanced but did not significantly different under higher TR condition. The result suggested that the supplied NH4+ coupled with enhanced transpiration was supposed to be more optimized option for IP assisted aquatic U rhizofiltration via P. australis.
Assuntos
Nitrogênio/metabolismo , Poaceae/fisiologia , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Biodegradação Ambiental , Transporte Biológico , Ferro/química , Raízes de Plantas/metabolismo , Rizoma , Poluentes Radioativos da Água/análise , Áreas AlagadasRESUMO
In order to develop an effective and economical method for removing low concentration radioactive wastewater of uranium, the biomass of 'CMCC(F)-98003' Aspergillus niger was investigated in a batch system. The maximum uranium adsorption capacity of 12.5 mg g-1 was obtained at the initial uranium concentration of 0.75 mg L-1. The biosorption data on a biomass concentration of 0.029 g L-1 fitted well to the Freundlich isotherm with a correlation coefficient (R2) of 0.987. The calculated thermodynamic parameters showed that the biosorption of uranium ions was endothermic (ΔH° < 0). The results of scanning electron microscope and Fourier transform infrared spectrometry analysis revealed that nano-particles of uranium precipitation were formed on the cell surfaces after biosorption, and the functional groups of -CH, N-H, -COOH, P = O and the carbohydrates and alcohols were involved in the biosorption process between A. niger and uranium ions.
Assuntos
Aspergillus niger/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Adsorção , Biodegradação Ambiental , Biomassa , Concentração de Íons de Hidrogênio , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Termodinâmica , Eliminação de Resíduos LíquidosRESUMO
Removal of toxic chemicals from environmental samples with low-cost methods and materials are very useful approach for especially large-scale applications. Green algae are highly abundant biomaterials which are employed as useful biosorbents in many studies. In the present study, an interesting type of green algae, Cladophora hutchinsiae (C. hutchinsiae) was used for removal of highly toxic chemical such as uranium. The pH, biosorbent concentration, contact time and temperature were optimized as 5.0, 12 g/L, 60 min and 20 °C, respectively. For the equilibrium calculations, three well known isotherm models (Langmuir, Freundlich and Dubinin-Radushkevich) were employed. The maximum biosorption capacity of the biosorbent was calculated as about 152 mg/g under the optimum batch conditions. The mean energy of biosorption was calculated as 8.39 kJ/mol from the D-R biosorption isotherm. The thermodynamic and kinetic characteristics of biosorption were also investigated to explain the nature of the process. The kinetic data best fits the pseudo-second-order kinetic model with a regression coefficient of >0.99 for all studied temperatures. The calculated ΔH° and ΔG° values showed that the biosorption process is exothermic and spontaneous for temperatures between 293 and 333 K. Furthermore, after seven cycling process, the sorption and desorption efficiencies of the biosorbent were found to be 70, and 58%, respectively meaning that the biosorbent had sufficiently high reusability performance as a clean-up tool.
Assuntos
Clorófitas/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Adsorção , Biomassa , Cinética , Modelos Teóricos , TermodinâmicaRESUMO
Batch experiments were conducted to investigate the biosorption of U(VI) onto Hottentot Fern (Cyclosorus interruptus). The selective adsorption, the adsorption of different sections of Cyclosorus interruptus (CI), and the adsorption of polluted CI compared with that of unpolluted one were studied in detail. The raw CI and the CI modified by CaCl2, MgCl2, MgCl2/H2O2 were investigated for adsorption of U(VI) from aqueous solution. The results indicate that raw CI showed good adsorption selectivity for U(VI), compared with the adsorption of Cu(II), Co(II) and Ni(II). The stem of CI possesses a prominent adsorption capacity compared to the leaf and root of CI, and the unpolluted CI showed its superiority in adsorption capacity than the polluted CI. Adsorption rate was very fast during the first 30 min in the whole adsorption process. The pseudo-second-order kinetics model was proposed for the adsorption of U(VI) and the equilibrium data fitted well to Langmuir adsorption isotherms. The maximum adsorption capacity of R-CI, Ca-CI, Mg-CI and Mg/H2O2-CI is 41.67, 52.63, 62.50 and 71.43 mg g-1 at 20 °C, respectively.
Assuntos
Gleiquênias/fisiologia , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Adsorção , Biodegradação Ambiental , Cinética , TermodinâmicaRESUMO
The present study highlights the uranium (U) concentrations in water-soil-plant matrices and the efficiency considering a heterogeneous assemblage of terrestrial and aquatic native plant species to act as the biomonitor and phytoremediator for environmental U-contamination in the Sevilha mine (uraniferous region of Beiras, Central Portugal). A total of 53 plant species belonging to 22 families was collected from 24 study sites along with ambient soil and/or water samples. The concentration of U showed wide range of variations in the ambient medium: 7.5 to 557mgkg(-1) for soil and 0.4 to 113µgL(-1) for water. The maximum potential of U accumulation was recorded in roots of the following terrestrial plants: Juncus squarrosus (450mgkg(-1) DW), Carlina corymbosa (181mgkg(-1) DW) and Juncus bufonius (39.9mgkg(-1) DW), followed by the aquatic macrophytes, namely Callitriche stagnalis (55.6mgkg(-1) DW) Lemna minor (53.0mgkg(-1) DW) and Riccia fluitans (50.6mgkg(-1) DW). Accumulation of U in plant tissues exhibited the following decreasing trend: root>leaves>stem>flowers/fruits and this confirms the unique efficiency of roots in accumulating this radionuclide from host soil/sediment (phytostabilization). Overall, the accumulation pattern in the studied aquatic plants (L. minor, R. fluitans, C. stagnalis and Lythrum portula) dominated over most of the terrestrial counterpart. Among terrestrial plants, the higher mean bioconcentration factor (≈1 in roots/rhizomes of C. corymbosa and J. squarrosus) and translocation factor (31 in Andryala integrifolia) were encountered in the representing families Asteraceae and Juncaceae. Hence, these terrestrial plants can be treated as the promising candidates for the development of the phytostabilization or phytoextraction methodologies based on the accumulation, abundance and biomass production.
Assuntos
Plantas/metabolismo , Poluentes Radioativos do Solo/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Biodegradação Ambiental , Monitoramento Ambiental , Recuperação e Remediação Ambiental , Sedimentos Geológicos/análise , Mineração , Portugal , Poluentes Radioativos do Solo/análise , Urânio/análise , Poluentes Radioativos da Água/análiseRESUMO
Phytoremediation potential of uranium (U) was investigated by submerged, free-floating and rooted emergent native aquatic macrophytes inhabiting along the streams of Horta da Vilariça, a uraniferous geochemical region of NE Portugal. The work has been undertaken with the following objectives: (i) to relate the U concentrations in water-sediment-plant system; and (ii) to identify the potentialities of aquatic plants to remediate U-contaminated waters based on accumulation pattern. A total of 25 plant species culminating 233 samples was collected from 15 study points along with surface water and contiguous sediments. Concentrations of U showed wide range of variations both in waters (0.61-5.56 µg L(-1), mean value 1.98 µg L(-1)) and sediments (124-23,910 µg kg(-1), mean value 3929 µg kg(-1)) and this is also reflected in plant species examined. The plant species exhibited the ability to accumulate U several orders of magnitude higher than the surrounding water. Maximum U concentrations was recorded in the bryophyte Scorpiurium deflexifolium (49,639 µg kg(-1)) followed by Fontinalis antipyretica (35,771 µg kg(-1)), shoots of Rorippa sylvestris (33,837 µg kg(-1)), roots of Oenanthe crocata (17,807 µg kg(-1)) as well as in Nasturtium officinale (10,995 µg kg(-1)). Scorpiurium deflexifolium displayed a high bioconcentration factor (BF) of â¼2.5 × 10(4) (mean value). The species Fontinalis antipyretica, Nasturtium officinale (roots) and Rorippa sylvestris (shoots) exhibited the mean BFs of 1.7 × 10(4), 5 × 10(3) and 4.8 × 10(3) respectively. Maximum translocation factor (TF) was very much pronounced in the rooted perennial herb Rorippa sylvestris showing extreme ability to transport U for the shoots and seems to be promising candidate to be used as bioindicator species.
Assuntos
Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Biodegradação Ambiental , Briófitas/metabolismo , Bryopsida/metabolismo , Monitoramento Ambiental , Nasturtium/metabolismo , Oenanthe/metabolismo , Raízes de Plantas/metabolismo , Brotos de Planta/metabolismo , Portugal , Rios/química , Rorippa/metabolismo , Urânio/análise , Poluentes Radioativos da Água/análiseRESUMO
In order to study the bioreduction of U(VI) and stability of immobilized uranium under suboxic conditions, microcosm were amended with ethanol, lactate and glucose, and incubated under suboxic conditions. During the incubation, total dissolved U in amended microcosms decreased from 0.95 mg/L to 0.03 mg/L. Pyrosequencing results showed that, the proportion of anaerobic microorganisms capable of reducing U(VI) under suboxic conditions was small compared with that under anoxic conditions; the proportion of aerobic and facultative anaerobic microorganisms capable of consuming the dissolved oxygen was large; and some of the facultative anaerobic microorganisms could reduce U(VI). These results indicated that different microbial communities were responsible for the bioreduction of U(VI) under suboxic and anoxic conditions. After the electron donors were exhausted, total dissolved U in the amended microcosms remained unchanged, while the U(VI)/U(IV) ratio in the solid phase of sediments increased obviously. This implied that the performance of bioreduction of the U(VI) can be maintained under suboxic condition.
Assuntos
Bactérias/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo , Anaerobiose , Bactérias/genética , Biodegradação Ambiental , DNA Bacteriano/genética , Sedimentos Geológicos/análise , Oxirredução , RNA Ribossômico 16S/genéticaRESUMO
Batch experiments were conducted to investigate the biosorption of U(VI) from aqueous solutions onto the nonliving biomass of an aquatic macrophyte Eichhornia crassipes. The results showed that the adsorption of U(VI) onto E. crassipes was highly pH-dependent and the best pH for U(VI) removal was 5.5. U(VI) adsorption proceeded rapidly with an equilibrium time of 30 min and conformed to pseudo-second-order kinetics. The Langmuir isotherm model was determined to best describe U(VI) biosorption with a maximum monolayer adsorption capacity of 142.85 mg/g. Thermodynamic calculation results indicated that the U(VI) biosorption process was spontaneous and endothermic. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis implied that the functional groups (amino, hydroxyl, and carboxyl) may be responsible for the U(VI) adsorption process, in which the coordination and ion exchange mechanisms could be involved. We conclude that E. crassipes biomass is a promising biosorbent for the removal of uranium pollutants.