RESUMO
It has become a common consensus that resource conservation and intensive recycling for improving resource utilization efficiency is an important way to achieve carbon peak and carbon neutrality(dual carbon). Traditonal Chinese medicine(TCM)resources as national strategic resources are the material basis and fundamental guarantee for the development of TCM industry and health services. However, the rapid growth of China's TCM industry and the continuous expansion and extension of the industrial chain have exposed the low efficiency of TCM resources. Resource waste and environmental pollution caused by the treatment and discharge of TCM waste have emerged as major problems faced by the development of the industry, which has aroused wide concern. Considering the dual carbon goals, this paper expounds the role and potential of TCM resource recycling and circular economy industry development. Taking the typical model of TCM resource recycling as the case of circular economy industry in reducing carbon source and increasing carbon sink, this paper puts forward the suggestions for the TCM circular economy industry serving the double carbon goals. The suggestions mainly include strengthening the policy and strategic leading role of the double carbon goals, building an objective evaluation system of low-carbon emission reduction in the whole industrial chain of TCM resources, building an industrial demonstration park for the recycling of TCM resources, and promoting the establishment of a circular economy system of the whole industrial chain of TCM resources. These measures are expected to guide the green transformation of TCM resource industry from linear economic model to circular economy model, provide support for improving the utilization efficiency and sustainable development of TCM resources, and facilitate the low-carbon and efficient development of TCM resource industry and the achievement of the double carbon goals.
Assuntos
Reutilização de Equipamento , Medicina Tradicional Chinesa , Objetivos , Poluição Ambiental , Desenvolvimento Econômico , Carbono , ChinaRESUMO
BACKGROUND AND AIMS: water is an imperfect agent for lens cleansing during endoscopy due to its incompetence to clean hydrophobic dirt, whereas amphiphilic surfactants have the potential to overcome the limitation of water. The trial was aimed to evaluate the cleansing effectiveness of 2 typical surfactants (simethicone solution and oolong tea) for colonoscopic lens. METHODS: Oolong tea (O-), low concentration simethicone solution (S1-), high concentration simethicone solution (S2-) and distilled water (D-) were used as washing solutions for colonoscopic lens. Study I: The tip of the colonoscope was immersed in lard oil in order to simulate the blur, and photographs were taken toward a standard colonoscopy image in-vitro pre- and post- each cleansing procedure. The blurred areas of each image were quantified and compared. Study II: 395 consecutive patients who were due to colonoscopy examination were enrolled and randomized into O-, S2-, D-group. The volume of washing solution used and cleansing level during the examination procedure, adenoma and polyp detected per colonoscopy, insertion time and withdraw time were analyzed. RESULTS: Study I: There were no differences in 4 groups for the blurred areas on images before lens cleansing. The blurred areas after lens cleansing were significantly smaller in 3 groups (O- 8.47â±â20.91 vs S1- 13.06â±â10.71 vs S2- 6.76â±â8.49 vs D- 38.24â±â29.69, Pâ<â.05) than water. The decline range of blurred areas after lens cleansing in oolong tea, low concentration simethicone solution, high concentration simethicone solution groups were significantly higher than that in distilled water group (O- 87.35â±â20.81 vs S1- 78.12â±â19.24 vs S2- 89.57â±â8.50 vs D- 53.39â±â28.45, Pâ<â.05). Study II: The volume of washing solution used in S2-group was significantly smaller than that in O-group and D-group. The cleansing level of the colonoscopic lens of O-group was significantly superior than that of S2-group and D-group. CONCLUSIONS: The in-vitro test showed oolong tea and simethicone solution can effectively cleans the colonoscopic lens. The clinical trial demonstrated that oolong tea instead of water is effective to provide better visualization during colonoscopy.Registration: Chictr.org.cn No: ChiCTR1900025606.
Assuntos
Colonoscopia/instrumentação , Manutenção/normas , Simeticone/uso terapêutico , Chá , Colonoscopia/métodos , Método Duplo-Cego , Reutilização de Equipamento/normas , Humanos , Manutenção/métodos , Manutenção/estatística & dados numéricos , Simeticone/farmacologia , Água/administração & dosagemRESUMO
Enzyme immobilization can increase enzyme reusability to reduce cost of industrial production. Ginkgo biloba leaf extract is commonly used for medical purposes, but it contains ginkgolic acid, which has negative effects on human health. Here, we report a novel approach to solve the problem by degrading the ginkgolic acid with immobilized-laccase, where core/shell composite nanoparticles prepared by coaxial electrospraying might be first applied to enzyme immobilization. The core/shell Fe3O4/nylon 6,6 composite nanoparticles (FNCNs) were prepared using one-step coaxial electrospraying and can be simply recovered by magnetic force. The glutaraldehyde-treated FNCNs (FNGCNs) were used to immobilize laccase. As a result, thermal stability of the free laccase was significantly improved in the range of 60-90 °C after immobilization. The laccase-immobilized FNGCNs (L-FNGCNs) were applied to degrade the ginkgolic acids, and the rate constants (k) and times (τ50) were ~0.02 min-1 and lower than 39 min, respectively, showing good catalytic performance. Furthermore, the L-FNGCNs exhibited a relative activity higher than 0.5 after being stored for 21 days or reused for 5 cycles, showing good storage stability and reusability. Therefore, the FNGCNs carrier was a promising enzyme immobilization system and its further development and applications were of interest.
Assuntos
Óxido Ferroso-Férrico/química , Proteínas Fúngicas/química , Ginkgo biloba/química , Lacase/química , Nanopartículas de Magnetita/química , Salicilatos/química , Reagentes de Ligações Cruzadas/química , Técnicas Eletroquímicas , Enzimas Imobilizadas/química , Enzimas Imobilizadas/isolamento & purificação , Reutilização de Equipamento , Proteínas Fúngicas/isolamento & purificação , Glutaral/química , Hidrólise , Cinética , Lacase/isolamento & purificação , Nanopartículas de Magnetita/ultraestrutura , Nylons/química , Extratos Vegetais/química , Folhas de Planta/química , Polyporaceae/química , Polyporaceae/enzimologiaAssuntos
Betacoronavirus/patogenicidade , Controle de Doenças Transmissíveis/normas , Infecções por Coronavirus/prevenção & controle , Pandemias/prevenção & controle , Fototerapia/normas , Pneumonia Viral/prevenção & controle , Guias de Prática Clínica como Assunto , Agendamento de Consultas , Betacoronavirus/efeitos da radiação , COVID-19 , Controle de Doenças Transmissíveis/instrumentação , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Descontaminação/métodos , Descontaminação/normas , Reutilização de Equipamento/normas , Humanos , Máscaras/normas , Fototerapia/instrumentação , Pneumonia Viral/epidemiologia , Pneumonia Viral/virologia , Prevalência , SARS-CoV-2 , Fatores de Tempo , Raios Ultravioleta , Inativação de Vírus/efeitos da radiaçãoRESUMO
For the first time, through a fast, eco-friendly and economic method, the aqueous extract of the leaf of Euphorbia corollate was used to the green synthesis of the highly stable CuO@Magnetite@Hen Bone nanocomposites (NCs) as a potent antioxidant and antibacterial agent against Pseudomonas aureus, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae pathogenic bacteria. The biosynthesised NCs were identified using the scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy, elemental mapping, X-ray diffraction (XRD), Fourier transforms infrared spectroscopy and UV-vis analytical techniques. Also, the radical scavenging activity using (2,2-diphenyl-1-picrylhydrazyl) method was used to evaluate the antioxidant activity of the NCs. The stability of nanocatalyst was monitored using the XRD and SEM analyses after 30 days from its synthesis. Furthermore, its excellent catalytic activity, recycling stability, and high substrate applicability were demonstrated to the adsorption of the polycyclic aromatic hydrocarbons of the light crude oil from Shiwashok oil fields and destruction of methylene blue and methyl orange as harmful organic dyes at ambient temperature using UV-vis spectroscopy. Moreover, the green CuO@Magnetite@Hen Bone NCs were recovered and reused several times without considerable loss of its catalytic activity.
Assuntos
Antibacterianos/síntese química , Antioxidantes/síntese química , Corantes/isolamento & purificação , Cobre/química , Nanopartículas de Magnetita/química , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Osso e Ossos/química , Galinhas , Corantes/metabolismo , Reutilização de Equipamento , Euphorbia/química , Feminino , Química Verde/métodos , Oxirredução , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Folhas de Planta/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismoRESUMO
A novel 3D metal-organic framework (MOF){[Tb3(CBA)2(HCOO)(µ3-OH)4(H2O)]·2H2O·0.5DMF} n (S-1) was synthesized by the solvothermal method. The crystal structure indicates that [Tb4O4] cubane clusters self-assemble into an infinite chain by sharing vertex, which is further linked to adjacent chains through 1,1-cyclobutanedicarboxylic acid ligand (H2CBA), resulting in a honeycomb arrayed framework. S-1 possesses excellent water stability and still retains intact structure after exposure to water for 10 weeks or boiling water for 10 weeks. Interestingly, S-1 acts as a luminescence sensor to selectively and sensitively detect quercetin with the limit of detection (LOD) as low as 0.23 ppm (7.6 × 10-7 M). The relationship between relative luminescence intensity and concentration obeys linear in the range of 0-300 ppm (0-993 µM), which allows quantitative detection of quercetin. Importantly, S-1 can be reused at least six times with almost no change in luminescent intensity. Compared with the high performance liquid chromatography-mass spectrometry (HPLC-MS) method, S-1 was used to determine the content of quercetin in onionskin and apple peel samples with satisfactory results. Furthermore, a portable S-1 test paper is also developed and expected to be applied in practice. To our knowledge, S-1 is the first example of MOFs as luminescent sensor for quercetin.
Assuntos
Substâncias Luminescentes/química , Estruturas Metalorgânicas/química , Quercetina/análise , Reutilização de Equipamento , Limite de Detecção , Substâncias Luminescentes/síntese química , Medições Luminescentes/instrumentação , Medições Luminescentes/métodos , Malus/química , Estruturas Metalorgânicas/síntese química , Cebolas/química , Papel , Térbio/químicaRESUMO
Antibiotics have been widely used in clinical applications to treat pathogenic infections at present, but the problem of drug-resistance associated with the abuse of antibiotics has become a large threat to human beings. Herein, we developed an antibacterial nanoagent by coating quaternized chitosan (QCS) on the surface of Fe3O4 nanoparticles-anchored graphene oxide (GO), which enabled QCS and GO to achieve synergistic effects on killing the drug-resistant bacteria. Systematical antibacterial experiments showed that the prepared nanoagent had antibacterial ability, which was significantly enhanced after the introduction of near-infrared (NIR). Importantly, the nanoagent could be easily recycled and reused without the reduction of the antibacterial ability. During the test time, this nanoagent exhibited no obviously toxic side effect to cells. Given the above advantages, we anticipate that the nanoagent has a promising future in various applications such as wound disinfection, water purification, and surface sterilization of medical devices.
Assuntos
Antibacterianos/química , Quitosana/química , Materiais Revestidos Biocompatíveis/química , Óxido Ferroso-Férrico/química , Grafite/química , Nanopartículas de Magnetita/química , Antibacterianos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Reutilização de Equipamento , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Óxido Ferroso-Férrico/farmacologia , Grafite/farmacologia , Células Endoteliais da Veia Umbilical Humana/citologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/fisiologia , Humanos , Raios Infravermelhos , Terapia com Luz de Baixa Intensidade/métodos , Nanopartículas de Magnetita/ultraestrutura , Óxidos , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimentoRESUMO
Lipases are the most widely employed enzymes in commercial industries. The catalytic mechanism of most lipases involves a step called "interfacial activation". As interfacial activation can lead to a significant increase in catalytic activity, it is of profound importance in developing lipase immobilization methods. To obtain a potential biocatalyst for industrial biodiesel production, an effective strategy for enhancement of catalytic activity and stability of immobilized lipase was developed. This was performed through the combination of interfacial activation with hybrid magnetic cross-linked lipase aggregates. This biocatalyst was investigated for the immobilization of lipase from Rhizomucor miehei (RML). Under the optimal conditions, the activity recovery of the surfactant-activated magnetic RML cross-linked enzyme aggregates (CLEAs) was as high as 2058%, with a 20-fold improvement over the free RML. Moreover, the immobilized RML showed excellent catalytic performance for the biodiesel reaction at a yield of 93%, and more importantly, could be easily separated from the reaction mixture by simple magnetic decantation, and retained more than 84% of its initial activities after five instances of reuse. This study provides a new and versatile approach for designing and fabricating immobilized lipase with high activation and stability.
Assuntos
Enzimas Imobilizadas/química , Proteínas Fúngicas/química , Jatropha/química , Lipase/química , Óleos de Plantas/química , Rhizomucor/química , Biocatálise , Biocombustíveis , Ativação Enzimática , Enzimas Imobilizadas/isolamento & purificação , Reutilização de Equipamento , Esterificação , Proteínas Fúngicas/isolamento & purificação , Lipase/isolamento & purificação , Imãs , Agregados Proteicos , Rhizomucor/enzimologia , Tensoativos/químicaRESUMO
BACKGROUND: Numerous published outbreaks, including one from our institution, have described endoscope-associated transmission of multidrug-resistant organisms (MDROs). Individual centers have adopted their own protocols to address this issue, including endoscope culture and sequestration. Endoscope culturing has drawbacks and may allow residual bacteria, including MDROs, to go undetected after high-level disinfection. AIM: To report the outcome of our novel protocol, which does not utilize endoscope culturing, to address our outbreak. METHODS: All patients undergoing procedures with elevator-containing endoscopes were asked to permit performance of a rectal swab. All endoscopes underwent high-level disinfection according to updated manufacturer's guidance. Additionally, ethylene oxide (EtO) sterilization was done in the high-risk settings of (1) positive response to a pre-procedure risk stratification questionnaire, (2) positive or indeterminate CRE polymerase chain reaction (PCR) from rectal swab, (3) refusal to consent for PCR or questionnaire, (4) purulent cholangitis or infected pancreatic fluid collections. Two endoscopes per weekend were sterilized on a rotational basis. RESULTS: From September 1, 2015 to April 30, 2016, 556 endoscopy sessions were performed using elevator-containing endoscopes. Prompted EtO sterilization was done on 46 (8.3%) instances, 3 from positive/indeterminate PCR tests out of 530 samples (0.6%). No CRE transmission was observed during the study period. Damage or altered performance of endoscopes related to EtO was not observed. CONCLUSION: In this pilot study, prompted EtO sterilization in high-risk patients has thus far eliminated endoscope-associated MDRO transmission, although no CRE infections were noted throughout the institution during the study period. Further studies and a larger patient sample will be required to validate these findings.
Assuntos
Carbapenêmicos/uso terapêutico , Colangiopancreatografia Retrógrada Endoscópica/instrumentação , Infecção Hospitalar/prevenção & controle , Surtos de Doenças/prevenção & controle , Farmacorresistência Bacteriana , Duodenoscópios/microbiologia , Endossonografia/instrumentação , Infecções por Enterobacteriaceae/prevenção & controle , Enterobacteriaceae/isolamento & purificação , Contaminação de Equipamentos/prevenção & controle , Reto/microbiologia , Adulto , Idoso , Infecção Hospitalar/diagnóstico , Infecção Hospitalar/microbiologia , Infecção Hospitalar/transmissão , Desinfetantes , Enterobacteriaceae/efeitos dos fármacos , Infecções por Enterobacteriaceae/diagnóstico , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/transmissão , Reutilização de Equipamento , Óxido de Etileno , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Projetos Piloto , Avaliação de Programas e Projetos de Saúde , Fatores de Risco , Esterilização/métodos , WisconsinAssuntos
Cateteres Cardíacos/normas , Técnicas Eletrofisiológicas Cardíacas/instrumentação , Reutilização de Equipamento/normas , Rotulagem de Produtos/normas , Brasil , Reutilização de Equipamento/legislação & jurisprudência , Órgãos Governamentais , Regulamentação Governamental , Humanos , Rotulagem de Produtos/legislação & jurisprudênciaRESUMO
INTRODUCTION & OBJECTIVES: Transurethral enucleation of the prostate for the management of benign prostatic obstruction (BPO) involves two steps: the enucleation and morcellation procedure. The aim of our study was to assess the efficacy of a morcellator device using disposable and reusable blades with different settings of morcellation speed. METHODS: A prospective randomized study was initiated for patients with symptomatic BPO undergoing Thulium laser enucleation of the prostate. Mechanical tissue morcellation was performed using the Piranha™ morcellator (R. Wolf, Knittlingen, Germany) with disposable or reusable blades at 850 (n = 24) or 1500 revolutions per minute (rpm) (n = 24). Patient characteristics, intraoperative complications, and the morcellation rate (g/min) were recorded. Data are expressed as median and interquartile range (IQR). RESULTS: Forty-eight patients were randomized using disposable (n = 24) or reusable blades (n = 24). For reusable blades, the morcellation rate did not increase when changing the morcellation speed from 850 to 1500 rpm (5 vs 4.53 g/min, p = 0.843). The morcellation rate increased significantly when changing the morcellation speed from 850 to 1500 rpm using single-use blades (4.77 vs 10 g/min, p ≤ 0.014). The morcellation rate was not different at 850 rpm between reusable and single-use blades (5 vs 4.77 g/min, p = 0.671). Conversely, the morcellation rate was significantly different at 1500 rpm between reusable and single-use blades (4.53 vs 10 g/min, p ≤ 0.017). The total morcellation rate (at 850 and 1500 rpm) was significantly increased using single-use blades compared to reusable blades (7.67 vs 4.8 g/min, p ≤ 0.026). Interestingly, enucleated weight (g) and the morcellation rate (g/min) correlated inversely using single-use blades at 1500 rpm (r = -0.742, p ≤ 0.004). Only one superficial bladder injury occurred at 1500 rpm, which needed no further interventions. CONCLUSIONS: The Piranha morcellator facilitates efficient tissue removal with single-use and reusable blades. Disposable morcellator blades increase tissue removal significantly at 1500 rpm.
Assuntos
Equipamentos Descartáveis , Reutilização de Equipamento , Terapia a Laser/métodos , Morcelação/instrumentação , Hiperplasia Prostática/cirurgia , Ressecção Transuretral da Próstata/instrumentação , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Estudos ProspectivosRESUMO
Bio-based catalyst support materials with high thermal and structural stability are desired for catalysts systems requiring harsh conditions. In this study, a thermally stable palladium catalyst (up to 440°C) was designed from sporopollenin, which occurs naturally in the outer exine layer of pollens and is widely acknowledged as chemically very stable and inert biological material. Catalyst design procedure included (1) extraction of sporopollenin microcapsules from Betula pendula pollens (â¼25µm), (2) amino-functionalisation of the microcapsules, (3) Schiff base modification and (4) preparation of Pd(II) catalyst. The catalytic activity of the sporopollenin microcapsule supported palladium catalyst was tested in catalysis of biaryls by following a fast, simple and green microwave-assisted method. We recorded outstanding turnover number (TON: 40,000) and frequency (TOF: 400,000) for the catalyst in Suzuki coupling reactions. The catalyst proved to be reusable at least in eight cycles. The catalyst can be suggested for different catalyst systems due to its thermal and structural durability, reusability, inertness to air and its eco-friendly nature.
Assuntos
Anisóis/química , Biopolímeros/química , Ácidos Borônicos/química , Carotenoides/química , Paládio/química , Bases de Schiff/química , Betula/química , Biopolímeros/isolamento & purificação , Cápsulas/química , Carotenoides/isolamento & purificação , Catálise , Técnicas de Química Sintética , Reutilização de Equipamento , Micro-Ondas , Pólen/químicaRESUMO
Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 µmol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K m value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.
Assuntos
Enzimas Imobilizadas/química , Glycine max/química , Pectinas/química , Ácido Periódico/química , Peroxidase/química , Proteínas de Plantas/química , Boroidretos/química , Reutilização de Equipamento , Hidrogéis/química , Peróxido de Hidrogênio/química , Concentração de Íons de Hidrogênio , Cinética , Oxirredução , Glycine max/enzimologia , Tiramina/química , ResíduosRESUMO
For the increasing attention focused on saving endangered environments, there is a growing need for developing membrane materials able to perform complex functions such as removing radioactive pollutants and oil spills from water. A major challenge is the scalable fabrication of membranes with good mechanical and thermal stability, superior resistance to radiation, and excellent recyclability. In this study, we constructed a multifunctional flexible free-standing sodium titanate nanobelt (Na-TNB) membrane that was assembled as advanced radiation-tainted water treatment and oil uptake. We compared the adsorption behavior of (137)Cs(+) and (90)Sr(2+) on Na-TNB membranes under various environmental conditions. The maximum adsorption coefficient value (Kd) for Sr(2+) reaches 10(7) mL g(-1). The structural collapse of the exchange materials were confirmed by XRD, FTIR and XPS spectroscopy as well as Raman analysis. The adsorption mechanism of Na-TNB membrane is clarified by forming a stable solid with the radioactive cations permanently trapped inside. Besides, the engineered multilayer membrane is exceptionally capable in selectively and rapidly adsorbing oils up to 23 times the adsorbent weight when coated with a thin layer of hydrophobic molecules. This multifunctional membrane has exceptional potential as a suitable material for next generation water treatment and separation technologies.
Assuntos
Radioisótopos de Césio/isolamento & purificação , Óxidos/química , Petróleo/análise , Radioisótopos de Estrôncio/isolamento & purificação , Titânio/química , Poluentes Químicos da Água/isolamento & purificação , Poluentes Radioativos da Água/isolamento & purificação , Adsorção , Reutilização de Equipamento , Humanos , Interações Hidrofóbicas e Hidrofílicas , Cinética , Teste de Materiais , Membranas Artificiais , Nanoestruturas/química , Poluição por Petróleo , Liberação Nociva de Radioativos , Água do Mar/química , Purificação da Água/instrumentação , Purificação da Água/métodosRESUMO
BACKGROUND/AIMS: The prevalence of hepatitis C virus (HCV) infection in Busan, Gyeongnam, and Jeonnam Provinces in Korea is more than twice the national average. This study aimed to examine whether demographic and lifestyle characteristics are associated with HCV infection in these areas. METHODS: A case control study was performed at three study hospitals. HCV cases were matched with two controls for sex and age. Patient controls were selected from non-HCV patients at the same hospital. Healthy controls were subjects participating in medical checkups. Conditional logistic regression models were used. RESULTS: A total of 234 matched-case and patient- and healthy-control pairs were analyzed. The significant risk factors for both controls were sharing razors (adjusted odds ratio [aOR], 2.39 and 3.29, respectively) and having more than four lifetime sexual partners (aOR, 2.15 and 6.89, respectively). Contact dockworkers (aOR, 1.91) and tattoos (aOR, 2.20) were significant risk factors for the patient controls. Transfusion (aOR, 5.38), a bloody operation (aOR, 5.02), acupuncture (aOR, 2.08), and piercing (aOR, 5.95) were significant risk factors for the healthy controls. Needle stick injuries and intravenous drug abuse were significant in the univariate analysis. CONCLUSIONS: More education concerning the dangers of sharing razors, tattoos and piercings is required to prevent HCV infection. More attention should be paid to needle stick injuries in hospitals and the community.
Assuntos
Hepacivirus , Hepatite C/epidemiologia , Hepatite C/transmissão , Terapia por Acupuntura/efeitos adversos , Adulto , Idoso , Piercing Corporal/efeitos adversos , Estudos de Casos e Controles , Reutilização de Equipamento , Feminino , Hepatite C/virologia , Humanos , Estilo de Vida , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Ferimentos Penetrantes Produzidos por Agulha/complicações , Ferimentos Penetrantes Produzidos por Agulha/virologia , Razão de Chances , Prevalência , República da Coreia/epidemiologia , Fatores de Risco , Comportamento Sexual , Parceiros Sexuais , Tatuagem/efeitos adversos , Reação Transfusional , Local de Trabalho , Adulto JovemRESUMO
BACKGROUND/AIMS: The prevalence of hepatitis C virus (HCV) infection in Busan, Gyeongnam, and Jeonnam Provinces in Korea is more than twice the national average. This study aimed to examine whether demographic and lifestyle characteristics are associated with HCV infection in these areas. METHODS: A case control study was performed at three study hospitals. HCV cases were matched with two controls for sex and age. Patient controls were selected from non-HCV patients at the same hospital. Healthy controls were subjects participating in medical checkups. Conditional logistic regression models were used. RESULTS: A total of 234 matched-case and patient- and healthy-control pairs were analyzed. The significant risk factors for both controls were sharing razors (adjusted odds ratio [aOR], 2.39 and 3.29, respectively) and having more than four lifetime sexual partners (aOR, 2.15 and 6.89, respectively). Contact dockworkers (aOR, 1.91) and tattoos (aOR, 2.20) were significant risk factors for the patient controls. Transfusion (aOR, 5.38), a bloody operation (aOR, 5.02), acupuncture (aOR, 2.08), and piercing (aOR, 5.95) were significant risk factors for the healthy controls. Needle stick injuries and intravenous drug abuse were significant in the univariate analysis. CONCLUSIONS: More education concerning the dangers of sharing razors, tattoos and piercings is required to prevent HCV infection. More attention should be paid to needle stick injuries in hospitals and the community.
Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Terapia por Acupuntura/efeitos adversos , Transfusão de Sangue/efeitos adversos , Piercing Corporal/efeitos adversos , Estudos de Casos e Controles , Reutilização de Equipamento , Hepacivirus , Hepatite C/epidemiologia , Estilo de Vida , Modelos Logísticos , Ferimentos Penetrantes Produzidos por Agulha/complicações , Razão de Chances , Prevalência , República da Coreia/epidemiologia , Fatores de Risco , Comportamento Sexual , Parceiros Sexuais , Tatuagem/efeitos adversos , Local de TrabalhoRESUMO
PURPOSE: To study a new technique for cleaning microcatheters for reuse after NBCA embolization ("NBCA"), and to evaluate the clinical reusability of microcatheters that were cleaned with gelatin sponge particles after NBCA. MATERIALS AND METHODS: Four cleaning solution flushes for microcatheters after NBCA injection-5 % glucose ("glucose") only, Lipiodol-glucose, gelatin sponge particles ("gelatin")-glucose, and Lipiodol-gelatin-glucose-were examined experimentally. These solutions were evaluated by performing three examinations: a microcatheter resistance test based on the time taken to pass water through the microcatheter, a microcatheter resistance test based on the ease of insertion of a microguidewire, and observations of the inner surfaces of the cylinders after NBCA. Microcatheters that had already been used in NBCA were cleaned using this new technique and then applied in 20 clinical sessions (19 patients). RESULTS: There was no significant difference in water passage time between the controls and the groups that received a gelatin flush. In the resistance test based on the insertion of a microguidewire, groups that received a gelatin flush showed significantly less resistance than the groups that did not receive a gelatin flush. Observations of the inner surfaces of the cylinders indicated that cleaning with gelatin can lead to inner surfaces that are almost indistinguishable from control surfaces in terms of cleanliness. All clinical procedures involving Lipiodol-gelatin-glucose flushes were performed without any technical difficulties or complications. CONCLUSIONS: Applying the new cleaning technique utilizing gelatin sponge particles to microcatheters after NBCA ensures that they are clean enough to be reused.
Assuntos
Catéteres , Detergentes/administração & dosagem , Embolização Terapêutica , Embucrilato , Óleo Etiodado/administração & dosagem , Esponja de Gelatina Absorvível/administração & dosagem , Glucose/administração & dosagem , Adulto , Idoso , Catéteres/normas , Embolização Terapêutica/instrumentação , Embolização Terapêutica/normas , Embucrilato/administração & dosagem , Reutilização de Equipamento/normas , Feminino , Humanos , Japão , Masculino , Microesferas , Pessoa de Meia-Idade , Pressão , Reprodutibilidade dos Testes , SeringasRESUMO
A facile, efficient and environmentally-friendly protocol has been developed for the green synthesis of CuO nanoparticles (NPs) by aqueous extract of Gundelia tournefortii as a mild, renewable and non-toxic reducing agent. CuO NPs were characterized by SEM, TEM, XRD, EDS, FT-IR and UV-vis spectroscopy. More importantly, the green synthesized CuO NPs presented excellent catalytic activity for reduction of 4-nitrophenol and synthesis of N-monosubstituted ureas via hydration of cyanamides with the aid of acetaldoxime as an effective water surrogate in ethanol as a green solvent. The catalyst was easily separated and the recovered catalyst was reused many times without any significant loss of the catalytic activity.
Assuntos
Asteraceae/química , Cobre/química , Química Verde , Nanopartículas/química , Nitrofenóis/química , Ureia/síntese química , Catálise , Cianamida/química , Reutilização de Equipamento , Nanopartículas/ultraestrutura , Oxirredução , Oximas/química , Extratos Vegetais/química , Ureia/análogos & derivadosRESUMO
As a carrier-free immobilization strategy, magnetic cross-linked enzyme aggregates (MCLEAs) showed improved enzyme activity, stability and magnetic response. In this study, MCLEAs of α-amylase (MCLEAs-amylase) was prepared under optimized conditions and characterized with scanning electron microscope and vibrating sample magnetometer. The prepared MCLEAs-amylase showed an amorphous structure and the saturation magnetization was 33.5emu/g, which was sufficient for magnetic separation. Then MCLEAs-amylase coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was utilized to screen and identify α-amylase inhibitors from ethyl acetate extract of corni fructus. The experiment conditions were optimized. At the optimum conditions (incubation time: 10min, pH: 7.0 and temperature: 20°C), querciturone was successfully screened and identified with weak non-specific binding. The screening result was verified by inhibition assays and the IC50 value of querciturone was 22.5µg/mL. This method provided a rapid way to screen active compounds from natural products.
Assuntos
Cornus/química , Inibidores Enzimáticos/análise , Inibidores Enzimáticos/isolamento & purificação , Enzimas Imobilizadas/química , Extratos Vegetais/química , alfa-Amilases/química , Cromatografia Líquida de Alta Pressão/métodos , Inibidores Enzimáticos/química , Inibidores Enzimáticos/metabolismo , Enzimas Imobilizadas/metabolismo , Reutilização de Equipamento , Nanopartículas de Magnetita/química , Espectrometria de Massas/métodos , alfa-Amilases/metabolismoRESUMO
OBJECTIVE: To evaluate in vitro the shear bond strength of brackets recycled by sandblasting with aluminum oxide particles of different sizes or reconditioned industrially after successive rebonding. MATERIALS AND METHODS: Eighty brackets were bonded and debonded sequentially three times. After the first debonding, brackets were divided into four groups: (group 1) sandblasting with aluminum oxide particles of 25 µ, (group 2) 50 µ, and (group 3) 110 µ, and (group 4) industrial recycling. Bond strength and adhesive material remaining on debonded bracket bases were evaluated for each successive debond. RESULTS: No significant differences were detected between the four groups following the first recycle (P > .05). After the second recycle, bond strength was significantly greater for the industrially recycled group than the other groups (P < .016). When shear bond strength was compared within each recycling method, the bond strength of sandblasted brackets decreased with the increase of particle size and with each recycle; for the industrially recycled group, no significant differences were detected between the three sequences (P > .016). In the evaluation of bond material remnant, the industrially recycled group left significantly less bond material after successive recycling than the other groups did (P < .016). Within each recycling method, the adhesive remnant decreased significantly after successive debond (P < .016). CONCLUSIONS: Industrial recycling obtained better results than sandblasting after three successive debondings. The brackets' shear bond strength decreased as the size of the aluminum oxide particle used for sandblasting increased and as recycling was repeated.