Photobiomodulation with 630-nm LED Inhibits M1 Macrophage Polarization via STAT1 Pathway Against Sepsis-Induced Acute Lung Injury.

Background: Sepsis-induced acute lung injury (ALI) is a clinical syndrome characterized by excessive uncontrolled inflammation. Photobiomodulation such as light-emitting diode (LED) irradiation has been used to attenuate inflammatory disease. Objective: The protective effect of 630 nm LED irradiation on sepsis-induced ALI remains unknown. The purpose of this study was to investigate the role of 630 nm LED irradiation in sepsis-induced ALI and its underlying mechanism. Methods and results: C57BL/6 mice were performed cecal ligation and puncture (CLP) for 12 h to generate experimental sepsis models. Histopathology analysis showed that alveolar injury, inflammatory cells infiltration, and hemorrhage were suppressed in CLP mice after 630 nm LED irradiation. The ratio of wet/dry weigh of lung tissue was significantly inhibited by irradiation. The number of leukocytes was reduced in bronchoalveolar lavage fluid. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) results and enzyme-linked immunosorbent assay showed that 630 nm LED irradiation significantly inhibited the mRNA and protein levels of M1 macrophage-related genes in the lung of CLP-induced septic mice. Meanwhile, LED irradiation significantly inhibited signal transducer and activator of transcription 1 (STAT1) phosphorylation in the lung of septic mice. In vitro experiments showed that 630 nm LED irradiation significantly inhibited M1 genes mRNA and protein expression in THP-1-derived M1 macrophages without affecting the cell viability. LED irradiation also significantly inhibited the level of STAT1 phosphorylation in THP-1-derived M1 macrophages. Conclusions: We concluded that 630 nm LED is promising as a treatment against ALI through inhibiting M1 macrophage polarization, which is associated with the downregulation of STAT1 phosphorylation.

[Rational prophylaxis of purulent-septic complications of the soft tissue in ambulatory-outpatient conditions].

The results of investigation are adduced, concerning impact of ultraviolet irradiation and electric field of ultrahigh frequency in conjunction with local application of 1% ethanol solution of chlorophyllipt, using bandage, on prevention of purulent-septic complications occurrence with the soft tissues affection. There were operated on 107 patients. In 56 patients the proposed scheme of purulent-septic complications prophylaxis was applied. This permits to exclude the systemic application of antibiotics for prophylaxis after "clean" operations performance.

[Laser photodynamic therapy of septic wounds].

Two comparable groups, 60 patients in each, were included in the study. Localization and area of the wounds, as well as basic treatment were equal in these groups. Patients of the main group received the additional photodynamic therapy with chlorine photosensibilizing agent. It led to the shortening of the wound clearing time and stimulation of the epithelisation together with fast decrease of bacterial load. Morphologic analysis showed faster inflammation decrease and granulation as well as lesser microcirculatory disorders in wounds treated with photodynamic therapy. Moreover, cicatrixes were more elastic and gentle in these patients.

[Ultraviolet irradiation of the blood in the complex treatment of suppurative-inflammatory diseases]. / Ultrafioletovoe obluchenie krovi bolnogo v kompleksnom lechenii gnoino-vospalitelnykh zabolevanii.

The experience with the use of ultraviolet irradiation (UVI) of the blood in 98 patients with purulent-inflammatory disease is presented. UVI of the blood has considerably improved the results of treatment of the patients. The highest effectiveness of UVI of the blood is noted in treatment of chromosepsis. The treatment of psoriasis by the mentioned method appeared ineffective.