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1.
Viruses ; 13(11)2021 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-34834977

RESUMO

Yersinia enterocolitica is a food-borne Gram-negative pathogen responsible for several gastrointestinal disorders. Host-specific lytic bacteriophages have been increasingly used recently as an alternative or complementary treatment to combat bacterial infections, especially when antibiotics fail. Here, we describe the proteogenomic characterization and host receptor identification of the siphovirus vB_YenS_ϕR2-01 (in short, ϕR2-01) that infects strains of several Yersinia enterocolitica serotypes. The ϕR2-01 genome contains 154 predicted genes, 117 of which encode products that are homologous to those of Escherichia bacteriophage T5. The ϕR2-01 and T5 genomes are largely syntenic, with the major differences residing in areas encoding hypothetical ϕR2-01 proteins. Label-free mass-spectrometry-based proteomics confirmed the expression of 90 of the ϕR2-01 genes, with 88 of these being either phage particle structural or phage-particle-associated proteins. In vitro transposon-based host mutagenesis and ϕR2-01 adsorption experiments identified the outer membrane vitamin B12 receptor BtuB as the host receptor. This study provides a proteogenomic characterization of a T5-type bacteriophage and identifies specific Y. enterocolitica strains sensitive to infection with possible future applications of ϕR2-01 as a food biocontrol or phage therapy agent.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Bactérias/metabolismo , Proteínas de Membrana Transportadoras/metabolismo , Siphoviridae/fisiologia , Yersinia/virologia , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/isolamento & purificação , Bacteriófagos/fisiologia , Genoma Viral , Proteômica , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/isolamento & purificação , Yersinia/genética , Yersinia enterocolitica/virologia
2.
J Antimicrob Chemother ; 74(4): 854-864, 2019 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30649322

RESUMO

OBJECTIVES: Antimicrobial resistance genes (ARGs) can be transferred by means of mobile genetic elements, which play a critical role in the dissemination of resistance in the bacterial community. ARG transmission within mobile genetic elements has been reported in plasmids and transposons but less frequently in bacteriophages. Here, the bacteriophage fraction of seven human faecal samples was purified and deep-sequenced to detect the presence of ARGs in the phage particles. METHODS: Seven faecal samples (five from healthy individuals and two from a patient before and after receiving ciprofloxacin treatment) were used to extract phage DNA, which was purified and then sequenced in a MiSeq (Illumina). Generated reads were checked for quality and assembled, and then the generated contigs analysed with Kraken, PHASTER, VirSorter and Prokka. Some genes were also validated by quantitative PCR. RESULTS: Analysis of the purified phage DNA by Kraken identified from 4 to 266 viruses in the samples. The viral fraction corresponded mainly to the order Caudovirales, including phages from the Siphoviridae and Myoviridae families. Bacterial genes associated with antimicrobial resistance were detected in the viral DNA, as confirmed by quantitative PCR. Higher densities of ARG-carrying phage particles were observed in the post- versus pre-ciprofloxacin treatment sample. CONCLUSIONS: The finding of ARGs in phage particles supports the description of phages as mobile elements contributing to the dissemination of bacterial antibiotic resistance and suggests ciprofloxacin treatment may play a role in the release of ARG-carrying particles, thereby increasing resistance.


Assuntos
Antibacterianos/administração & dosagem , Bacteriófagos/isolamento & purificação , Ciprofloxacina/administração & dosagem , Farmacorresistência Bacteriana , Fezes/virologia , Genes Bacterianos , Voluntários Saudáveis , Adulto , Idoso , Bacteriófagos/classificação , Bacteriófagos/genética , Biota/efeitos dos fármacos , DNA Viral/química , DNA Viral/genética , DNA Viral/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Pessoa de Meia-Idade , Myoviridae/classificação , Myoviridae/genética , Myoviridae/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/isolamento & purificação
3.
Appl Environ Microbiol ; 83(23)2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28939601

RESUMO

Providencia rettgeri is emerging as a new opportunistic pathogen with high antibiotic resistance. The need to find alternative methods to control antibiotic-resistant bacteria and the recent advances in phage therapy motivate the search for new phages able to infect Providencia spp. This study describes the isolation and characterization of an obligatory lytic phage, vB_PreS_PR1 (PR1), with therapeutic potential against drug-resistant P. rettgeri PR1 is a siphovirus. Its virion DNA size (118,537 bp), transcriptional organization, terminal repeats (10,461 bp), and nicks in the 3'-to-5' strand are similar to those of phage T5. However, sequence similarities of PR1 to phages of the T5virus genus at the DNA and protein levels are limited, suggesting that it belongs to a new species within the Siphoviridae family. PR1 exhibits the ability to kill P. rettgeri antibiotic-resistant strains, is highly specific to the species, and did not present known genomic markers indicating a temperate lifestyle. The lack of homologies between its proteins and proteins of the only other sequenced Providencia prophage, Redjac, suggests that these two phages evolved separately and may target different host proteins.IMPORTANCE The alarming increase in the number of bacteria resistant to antibiotics has been observed worldwide. This is particularly true for Gram-negative bacteria. For certain of their strains, no effective antibiotics are available. Providencia sp. has been a neglected pathogen but is emerging as a multidrug-resistant bacterium. This has revived interest in bacteriophages as alternative therapeutic agents against this bacterium. We describe the morphological, physiological, and genomic characterization of a novel lytic virus, PR1, which is able to kill drug-resistant P. rettgeri clinical isolates. Genomic and phylogenetic analyses indicate that PR1 is a distant relative of T5virus genus representatives. The lack of known virulence- or temperate lifestyle-associated genes in the genome of PR1 makes this phage a potential candidate for therapeutic use. Analysis of its genome also improves our knowledge of the ecology and diversity of T5-like siphoviruses, providing a new link for evolutionary studies of this phage group.


Assuntos
Bacteriófagos/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Providencia/virologia , Siphoviridae/isolamento & purificação , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/fisiologia , Terapia Biológica , Infecções por Enterobacteriaceae/terapia , Genoma Viral , Humanos , Filogenia , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/fisiologia
4.
Braz J Microbiol ; 46(3): 791-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26413062

RESUMO

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.


Assuntos
Bacteriófagos/isolamento & purificação , Agentes de Controle Biológico/isolamento & purificação , Dickeya chrysanthemi/crescimento & desenvolvimento , Dickeya chrysanthemi/virologia , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Bacteriófagos/classificação , Sequência de Bases , Agentes de Controle Biológico/classificação , DNA Bacteriano/genética , Dickeya chrysanthemi/efeitos dos fármacos , Dickeya chrysanthemi/isolamento & purificação , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Myoviridae/classificação , Myoviridae/isolamento & purificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Siphoviridae/classificação , Siphoviridae/isolamento & purificação
5.
Braz. j. microbiol ; 46(3): 791-797, July-Sept. 2015. tab, ilus
Artigo em Inglês | LILACS | ID: lil-755811

RESUMO

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.

.


Assuntos
Bacteriófagos/isolamento & purificação , Agentes de Controle Biológico/isolamento & purificação , Dickeya chrysanthemi/crescimento & desenvolvimento , Dickeya chrysanthemi/virologia , Doenças das Plantas/microbiologia , Solanum tuberosum/microbiologia , Sequência de Bases , Bacteriófagos/classificação , Agentes de Controle Biológico/classificação , DNA Bacteriano/genética , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Myoviridae/classificação , Myoviridae/isolamento & purificação , Dickeya chrysanthemi/efeitos dos fármacos , Dickeya chrysanthemi/isolamento & purificação , /genética , Análise de Sequência de DNA , Siphoviridae/classificação , Siphoviridae/isolamento & purificação
6.
Virology ; 443(2): 187-96, 2013 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-23755967

RESUMO

Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli/Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure.


Assuntos
Bacteriófagos , Terapia Biológica/efeitos adversos , Terapia Biológica/métodos , Infecções por Escherichia coli/terapia , Metagenômica/métodos , Infecções por Proteus/terapia , Administração Oral , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/ultraestrutura , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/virologia , Humanos , Microscopia Eletrônica de Transmissão , Myoviridae/classificação , Myoviridae/genética , Myoviridae/ultraestrutura , Podoviridae/classificação , Podoviridae/genética , Podoviridae/ultraestrutura , Federação Russa , Siphoviridae/classificação , Siphoviridae/genética , Siphoviridae/ultraestrutura , Resultado do Tratamento
7.
Curr Microbiol ; 61(4): 315-21, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20204638

RESUMO

Four phages infectious to Mesorhizobium strains were identified in soil samples taken from local Robinia pseudoacacia stands. Based on their polyhedral heads and short noncontractile tails, three of the phages, Mlo30, Mam12, and Mam20, were assigned to group C of Bradley's classification, the Podoviridae family, while phage Mlo1, with its elongated hexagonal head and a long flexible tail represented subgroup B2 bacteriophages, the Siphoviridae family. The phages were homogeneous in respect of their virulence, as they only lysed Mesorhizobium strains, but did not affect strains of Rhizobium or Bradyrhizobium. On the basis of one-step growth experiments, the average virus yield was calculated as approximately 10-25 phage particles for phages Mlo30, Mam12 and Mam20, and as many as 100-120 for phage Mlo1. The rate of phage adsorption to heat-treated cells showed differences in the nature of their receptors, which seemed to be thermal sensitive, thermal resistant, or a combination of the two. Only the receptor for phage Mlo30 was likely to be an LPS molecule, which was supported by a neutralization test. The smooth LPS with O-antigenic chains of the phage-sensitive M. loti strain completely reduced the bactericidal activity of virions at a concentration of 1 µg/ml. The molecular weights of phage DNAs estimated from restriction endonuclease cleavage patterns were in the range from approximately 39 kb for group C phages to approximately 80 kb for B2.


Assuntos
Alphaproteobacteria/virologia , Bacteriófagos/fisiologia , Bacteriófagos/ultraestrutura , DNA Viral/análise , Rizosfera , Robinia/microbiologia , Adsorção , Alphaproteobacteria/fisiologia , Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Bradyrhizobium/fisiologia , Bradyrhizobium/virologia , Clonagem Molecular , Microscopia Eletrônica , Fixação de Nitrogênio , Podoviridae/classificação , Podoviridae/isolamento & purificação , Podoviridae/fisiologia , Podoviridae/ultraestrutura , Rhizobium/fisiologia , Rhizobium/virologia , Siphoviridae/classificação , Siphoviridae/isolamento & purificação , Siphoviridae/fisiologia , Siphoviridae/ultraestrutura , Solo , Microbiologia do Solo , Simbiose , Vírion/ultraestrutura , Ligação Viral
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